AU2021100693A4 - A method to prepare water activity regulator used for minced meat slice products and its application - Google Patents

A method to prepare water activity regulator used for minced meat slice products and its application Download PDF

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AU2021100693A4
AU2021100693A4 AU2021100693A AU2021100693A AU2021100693A4 AU 2021100693 A4 AU2021100693 A4 AU 2021100693A4 AU 2021100693 A AU2021100693 A AU 2021100693A AU 2021100693 A AU2021100693 A AU 2021100693A AU 2021100693 A4 AU2021100693 A4 AU 2021100693A4
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water activity
minced meat
activity regulator
meat slice
slice
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AU2021100693A
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Chuanai Cao
Jiaxin Chen
Qian Chen
Baohua Kong
Qian Liu
Fangda Sun
Hao Wang
Hui Wang
Xiufang Xia
Hongwei Zhang
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Northeast Agricultural University
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Northeast Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/422Addition of natural plant hydrocolloids, e.g. gums of cellulose derivatives or of microbial fermentation gums
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B4/00General methods for preserving meat, sausages, fish or fish products
    • A23B4/14Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/426Addition of proteins, carbohydrates or fibrous material from vegetable origin other than sugars or sugar alcohols
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/269Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of microbial origin, e.g. xanthan or dextran
    • A23L29/271Curdlan; beta-1-3 glucan; Polysaccharides produced by agrobacterium or alcaligenes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/37Sugar alcohols
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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  • Wood Science & Technology (AREA)
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  • Meat, Egg Or Seafood Products (AREA)

Abstract

The invention relates to a method to prepare water activity regulator used for minced meat slice products and its application, belonging to the technical field of food additives. The water activity regulator for minced meat slice of the invention comprises the following ingredients (w/w): 2-6% curdlan gum, 5-15% glycerol and 10-20% corn protein hydrolysate. The invention provides an easy and effective water activity regulator for minced meat slice while improving its the tenderness and taste, it also has a remarkable effect of inhibiting fat oxidation during storage time.

Description

A method to prepare water activity regulator used for minced meat slice products and its application
TECHNICAL FIELD
The invention relates to a method to prepare water activity regulator used for minced meat slice products and its application, belonging to the technical field of food additives.
BACKGROUND
The minced meat slice is a traditional leisure meat product in China. In recent years, the. minced meat slice industry has become one of the fastest growing sectors in the field of meat processing. The. minced meat slice is made by grinding meat into minced meat slice, adding seasoning, marinating and scraping the minced meat on a sieve, drying and baking. Minced meat slice is a new kind of dried meat, which does not need the intact meat as raw material rather using effectively the minced meat, thus greatly reducing its production cost.
Minced meat slice product is storable, small in size and light in weight, it is convenient to transport and carry and high in protein and rich in nutrition. However, nowadays the minced meat slice products are plateaued with series problems such as rough texture, being too stiff for chewing, poor taste along with quality deterioration caused by microorganisms or oxidation during storage.
The existing technology used to solve the problem mentioned above includes: 1) Using ultrasonic, ultra-high pressure and protease to pre-treat the raw meat for improving the tenderness of minced meat slice products, 2) Adding vegetable and fruit ingredients (such as carrots, apples, celery, tomatoes, etc.) in minced meat slice products to improve color retention and tenderness of the meet, 3) Baking at low, medium and high temperature to promote the quality of minced meat slice products; 4) Adding preservatives and antioxidants to prolong the expiration date of minced meat slice products. However, in order to ensure the quality of minced meat slice products, it is necessary to combine the above methods, the preparation process is complicated.
SUMMARY
The purpose of the invention is to provide an effective and practical water activity regulator for minced meat slice products. With simple operational process required, the water activity regulator provided by the current invention can improve lower water activity, shear force and relaxation time of the minced meat slice product, and increase the moisture content, bacteriostasis and oxidation inhibition of the product at the same time.
This invention provides a water activity regulator comprises the ingredients (w/w): 2-6% curdlan gum, 5-15% glycerol and 10-20% corn protein hydrolysate.
Preferably, it comprises the ingredients (w/w): 4-5% curdlan gum, 8-12% glycerol and 12 16% corn protein hydrolysate.
This invention provides a minced meat slice with described water activity regulator that is characterized in comprising the ingredients (w/w): 90-120% raw meat and 3-5% water activity regulator.
This invention provides a method of preparing the described minced meat slice that comprises the following steps:
Adding the water activity regulator into raw meat, marinating, chopping, scraping the minced meat on a sieve and baking to obtain minced meat slice.
Preferably, the time for marinating is 6-12h.
Preferably, the mass ratio of the water activity regulator to the raw meat is 3.5:100.
This invention provides a water activity regulator comprises the ingredients (w/w): 2-6% curdlan gum, 5-15% glycerol and 10-20% corn protein hydrolysate. According to the invention, The curdlan gum combines with muscle protein to form a certain network structure, which promote the system to better retain the water; glycerol improves the water holding capacity of the system and reduces the water activity; corn protein hydrolysate provides a large number of carboxyl groups that interacts with protein in meat to hold the water. The water activity regulator provided by this invention can both increase the water content and reduce the water activity and improve the water distribution at the same time, ensuring the product own moderate hardness, better flavor, appearance, color retention and quality of the product, it can also effectively inhibit the oxidation of lipid and protein during the storage time.
According to the invention, the water activity regulator is used as a raw material, which is processed with raw meat to obtain the minced meat slice product.
The minced meat slice product provided by this invention has the feature of low water activity, low shear force and relaxation time, high water content, good bacteriostasis and oxidation inhibition capability, and having great tenderness and taste.
DESCRIPTION OF THE INVENTION
This invention provides a water activity regulator comprises the ingredients (w/w): 2-6% curdlan gum, 5-15% glycerol and 10-20% corn protein hydrolysate.
The water activity regulator provided by this invention includes 2-6% curdlan gum, preferably 3-5%, more preferably 3.5%. According to the invention, the curdlan gum combines with muscle protein to form a certain network structure, which promote the system to better retain the water. This invention does not particularly limit the source of curdlan gum, the commercially available curdlan gum which is well known to those skilled in the art can be adopted.
The water activity regulator provided by this invention include, 5-15% glycerol, preferably 8-12%, more preferably 10%. According to this invention, glycerol can improve the water holding capacity of the system and reduce the water activity; This invention does not particularly limit the source of glycerol, the commercially available glycerol which is well known to those skilled in the art can be adopted.
The water activity regulator provided by this invention includes 10-20% corn protein hydrolysate, preferably, 12-16%, more preferably, 15%. According to this invention, corn protein hydrolysate provides a large number of carboxyl groups that interacts with protein in meat to hold the water.
According to this invention, the preparation method for the described corn protein hydrolysate comprises the following steps:
Processing enzymatic hydrolysis of corn protein and alkaline protease at 40-55°C for 4-6 h;
Ultrafiltering the feed liquid obtained by enzymolysis to obtain filtrate with molecular weight less than 3kDa;
Desalting and drying the filtrate to obtain corn protein hydrolysate.
According to the invention, corn protein and alkaline protease are mixed for enzymolysis reaction; In the embodiment of the present invention, alkaline protease can be specifically added to corn protein. In the present invention, the temperature of the enzymolysis reaction is preferably 40-55°C, more preferably 50°C; The enzymolysis reaction time is preferably 4-6h, more preferably 5h.
In the present invention, the corn protein is preferably corn protein powder, and the source of the corn protein powder is not particularly limited in the present invention, and conventional market products of corn protein powder well known to those skilled in the art can be adopted. In the present invention, the alkaline protease is not particularly limited, and the alkaline protease well known to those skilled in the art can be adopted. In the present invention, the mass ratio of the alkaline protease to the corn protein is preferably 1: 45-60, and more preferably 1:50.
According to the invention, the enzymolysis is preferably carried out under the condition that the pH value is 7.8-8.5, and the pH adjustment is preferably carried out by using 1 mol/L NaOH; According to the invention, after the enzymolysis reaction, the pH value of the obtained feed liquid is preferably adjusted to 7.0 preferably by 1 mol/L hydrochloric acid.
After the enzymolysis, the invention preferably carries out enzyme inactivation treatment on the reaction system to obtain enzymolysis products. The method for inactivating enzyme in the present invention is not particularly limited, and conventional inactivating methods well known to those skilled in the art can be adopted, such as boiling water bath sterilization for 10 min.
After obtaining the enzymolysis product, the invention preferably ultrafilters the obtained feed liquid to obtain filtrate with molecular weight less than 3kDa. Before the ultrafiltration, the feed liquid is preferably centrifuged, and the supernatant obtained by centrifugation is ultrafiltered. Preferably, the centrifugal force is 8000-10000 g, more preferably 9000 g; ;The centrifugation time is preferably 15-40 min, more preferably 20-30 min.
There is no special restriction on the ultrafiltration in the present invention, and ultrafiltration can be performed by using an ultrafiltration membrane well known to those skilled in the art;
After ultrafiltration, desalting and drying the obtained filtrate with molecular weight less than 3kDa to obtain corn protein hydrolysate. In the present invention, there is no special limitation on the desalination, and the desalination operation well known to those skilled in the art can be adopted. In the present invention, the drying method is not particularly limited, and a drying method well known to those skilled in the art can be used, for example, freeze drying is preferred.
There is no special limitation on the preparation method of the water activity regulator in the present invention, and the preparation method of the composition well known to those skilled in the art can be adopted, for example, the weighed curdlan, glycerol and corn protein hydrolysate are mixed to make the water activity regulator. The application of the water activity regulator is not particularly limited in the present invention, and the use method of food additives well known to those skilled in the art can be adopted. In the embodiment of the present invention, it is preferable to directly add the water activity regulator to raw meat for marinating.
The invention uses water activity regulator described above as one of the raw materials to make a kind of minced meat slice, which comprises the following ingredients (w/w): 90-120% raw meat and 3-5% water activity regulator. The minced meat slice of the present invention preferably comprises 90-120% raw meat, preferably 95-110%, and most preferably 100%. The raw meat is not particularly limited in the invention, and the raw meat that is well known to those skilled in the art can be adopted.
The minced meat slice of the present invention preferably comprises 3-5%, more preferably 3.5% the water activity regulator described in the above technical methods
The minced meat slice of the present invention preferably further comprises seasonings, which are not particularly limited in the present invention, and can be combined with conventional meat seasonings.
The invention also provide a preparation method of that minced meat slice according to the technical scheme described, which comprises the following step: Adding the water activity regulator mentioned in the technical scheme to the raw meat for marinating, chopping, scraping the minced meat on a sieve and baking to obtain the minced meat slice.
According to the invention, the water activity regulator mentioned in the technical scheme is preferably added to the raw meat for pickling. In the present invention, the curing time is preferably 6-12h hours, preferably 8 hours. In the present invention, the mass ratio of the water activity regulator to the raw meat is preferably 3.5:100.
According to the invention, the raw meat is preferably chopped and mixed after marinating, and the conditions for chopping and mixing are not particularly limited in the invention, and the conditions of chopping and mixing methods well known to those skilled in the art can be adopted.
After chopping, the present invention preferably performs scraping the minced meat on a sieve and baking, which are not particularly limited in the present invention, and can be done by smearing and baking methods well known to those skilled in the art.
Herein to explain the invention of a regulator for reducing water activity of minced meat slice, minced meat slice and preparation method thereof in further detail with specific examples. The technical scheme of the present invention includes but is not limited to the following examples.
Embodiment 1
Selecting chicken and pork as raw materials to make minced meat slice.
Taking 1kg of chicken and pork with a mass ratio of 2:1 as main raw materials, cleaning and marinating: adding 300g of seasonings, including 1.5% salt, 0.01% sodium nitrite, 3.5%. light-colored soy sauce, 0.15% monosodium glutamate, 12% white sugar, 0.5% ginger powder, 0.5% white pepper, 2% white wine and 0.03% sodium erythorbate, 2% pig back fat and 8% crushed ice, 38g of water activity regulator ( Use the mass of water activity regulator as datum, the water activity regulator comprises 4% curdlan gum, 8% glycerol and 16% corn protein hydrolysate). After marinating, start the process of chopping, smearing, baking, cooling and vacuum packaging to obtain minced meat.
Embodiment 2
Selecting beef as raw materials to make minced meat slice.
Taking 5kg of beef as main raw materials, cleaning and marinating: adding 1.2kg of seasonings, including 2.2% salt, 0.015% sodium nitrite, 6%. light-colored soy sauce, 0.25% monosodium glutamate, 8% white sugar, 0.4% ginger powder, 0.2%. white pepper, 1.5% white wine and 0.06% sodium erythorbate, 3% pig back fat and 12% crushed ice, 220g of water activity regulator (Use the mass of water activity regulator as datum, the water activity regulator comprises 5% curdlan gum, 6% glycerol and 18% corn protein hydrolysate). After marinating, start the process of chopping, smearing, baking, cooling and vacuum packaging to obtain minced meat.
Embodiment 3
Selecting pork as raw materials to make minced meat slice.
Taking2 kg of lean pork as main raw materials, cleaning and marinating: adding 500g of seasonings, including 2% salt, 0.01% sodium nitrite, 5% light-colored soy sauce, 0.2% monosodium glutamate, 10% white sugar, 0.3% ginger powder, 0.3% white pepper, 1% white wine and 0.05% sodium erythorbate, 5% pig back fat and 10% crushed ice, 70g of water activity regulator (Use the mass of water activity regulator as datum, the water activity regulator comprises 6% curdlan gum, 10% glycerol and 15% corn protein hydrolysate). After marinating, start the process of chopping, smearing, baking, cooling and vacuum packaging to obtain minced meat.
All conditions of the control group were the same as those of embodiment 3, except no adding water activity regulator to dried meat products.
The indexes of minced meat slice added with moisture regulator were compared with those of control group. The comparison results are shown in Table 1 and Table 2. The water activity, shear force and relaxation time T21 decreased significantly (P < 0.05), while the water content and L*- value increased significantly (P< 0.05). After six months of storage, it was found that the sensory score of dried beef products added with moisture regulator was significantly higher than that of the control group (P < 0.05), while the POV value and TBARS value were significantly lower than those of the control group (P< 0.05). Combined with the above results, it was shown that moisture regulator can not only improve the quality of products, but also inhibit lipid oxidation.
Tablet 1 Comparison of various indexes between treatment group and control group on the day 0.
Water Project Content/% Water Activity Shear force/N L* a* b*
Control 25.11±1.3 0.871±0.002a 43.55±0.91a 3 3 .2 8 ±0. 3 6b 16.13±0.14a 15.11±0.41a Group
Treatment 30.54±0.4 0 .8 3 2 ±0.0 0 8b 2 2 .3 5 ±0.7 7b 36.33±0.82a 15.11+0.82a 13.79±0.36a Group 0a
Total number of Colony Overall acceptability/score T21/ms POV/meq-kg-1 TBARS/mg-kg-1 /log Cfu-g-1
Control 8.07±0. la 14.50+0.20a 1.86±0.26a 1.06±0.15a 2.83±0.26a Group
Treatment 8. 12 ±0.0 8b 1.39±0.23a 0.80±0.19a 2.37±0.24a Group 8.32±0.10a
Tablet 2 Comparison of various indexes between treatment group and control group in the 6th
month time.
Water Project Content/% Water Activity Shear force/N L* a* b*
Control 23.39±1. 0.891±0.007a 35.55±1.48a 3 0.4 3 ±0. 3 3 b 12.41±0.43a 12.49±0.56a Group 48 b
Treatment 27.95+0. 0 .8 4 3 ±0.0 0 7 1 8 .9 0 ±0.9 8b 33.57±0.46a 13.60±0.28a 11.47±0.34a Group 17a
Total number of Colony Overall acceptability/score T2 1/ms POV/meqkg-1 TBARS/mg.kg-' /log Cfu-g-1
Control 6. 10 ±0. 3 6 b 11.79g0.09a 19.65±0.84a 8.67±0.24a 7.39±0.42a Group
Treatment ± .08b . 1 0 ±0 .8 4 b 5.70±0.42b 6.43±0.22b Group 7.00±0.20a 5 .7 9 10
The above is only the preferred embodiment of the present invention, and it should be pointed out that for ordinary people in the technical field, without departing from the principle of the present invention, several improvements and embellishments can be made, and these improvements and embellishments should also be regarded as the protection scope of the present invention.

Claims (6)

THE CLAIMS DEFINING THE INVENTION ARE AS FOLLOWS:
1. A water activity regulator for minced meat slice is characterized in comprising the following ingredients (w/w): 2-6% curdlan gum, 5-15% glycerol and 10-20% corn protein hydrolysate.
2. According to claim 1, the water activity regulator is characterized in comprising the following ingredients (w/w): 4-5% curdlan gum, 8-12% glycerol and 12-16% corn protein hydrolysate.
3. According to any one of claims 1 to 2, a minced meat slice product with the water activity regulator is characterized in that the minced meat slice product comprises the following ingredients (w/w): 90-120% raw meat and 3-5% water activity regulator.
4. According to claim 3, the method for preparing minced meat slice comprises the following steps: adding the water activity regulator according to either one of claims 1 to 2 into raw meat, marinating, chopping, scraping the minced meat on a sieve and baking to obtain minced meat slice.
5. According to claim 4, the preparation method is characterized in that the marinating time is 6-12 h.
6. According to claim 4, the preparation method is characterized in that the mass ratio of the water activity regulator to the raw meat is 3.5:100.
AU2021100693A 2021-02-04 2021-02-04 A method to prepare water activity regulator used for minced meat slice products and its application Ceased AU2021100693A4 (en)

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