AU2020349513A1 - Optimized tetrahydrocannabinolic acid (THCA) synthase polypeptides - Google Patents

Optimized tetrahydrocannabinolic acid (THCA) synthase polypeptides Download PDF

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Publication number
AU2020349513A1
AU2020349513A1 AU2020349513A AU2020349513A AU2020349513A1 AU 2020349513 A1 AU2020349513 A1 AU 2020349513A1 AU 2020349513 A AU2020349513 A AU 2020349513A AU 2020349513 A AU2020349513 A AU 2020349513A AU 2020349513 A1 AU2020349513 A1 AU 2020349513A1
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AU
Australia
Prior art keywords
seq
polypeptide
host cell
modified host
amino acid
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Pending
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AU2020349513A
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English (en)
Inventor
Andrew HORWITZ
Darren Platt
Jeff UBERSAX
Jeff Wong
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Demetrix Inc
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Demetrix Inc
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Publication date
Application filed by Demetrix Inc filed Critical Demetrix Inc
Publication of AU2020349513A1 publication Critical patent/AU2020349513A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
    • C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
    • C12Y121/03007—Tetrahydrocannabinolic acid synthase (1.21.3.7)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2020349513A 2019-09-18 2020-09-17 Optimized tetrahydrocannabinolic acid (THCA) synthase polypeptides Pending AU2020349513A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962902300P 2019-09-18 2019-09-18
US62/902,300 2019-09-18
PCT/US2020/051261 WO2021055597A1 (en) 2019-09-18 2020-09-17 Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides

Publications (1)

Publication Number Publication Date
AU2020349513A1 true AU2020349513A1 (en) 2022-05-05

Family

ID=72744852

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020349513A Pending AU2020349513A1 (en) 2019-09-18 2020-09-17 Optimized tetrahydrocannabinolic acid (THCA) synthase polypeptides

Country Status (9)

Country Link
EP (1) EP4031657A1 (he)
JP (1) JP2022548904A (he)
CN (1) CN115038786A (he)
AU (1) AU2020349513A1 (he)
BR (1) BR112022004797A2 (he)
CA (1) CA3152803A1 (he)
IL (1) IL291328A (he)
MX (1) MX2022003203A (he)
WO (1) WO2021055597A1 (he)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020176547A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
WO2023168266A2 (en) * 2022-03-02 2023-09-07 Genomatica, Inc. Flavin-dependent oxidases having cannabinoid synthase activity

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA05003382A (es) 2002-10-04 2005-06-22 Du Pont Proceso para la produccion biologica de 1,3-propanodiol con alto rendimiento.
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
MX2010011706A (es) 2008-04-23 2011-03-15 Danisco Us Inc Variantes de isopreno-sintasa para produccion microbiana mejorada de isopreno.
JP2011526790A (ja) 2008-07-02 2011-10-20 ダニスコ・ユーエス・インク 分離された条件下および／または安全操業条件下における、ｃ５炭化水素を含まないイソプレン組成物及びその製造方法
WO2018200888A1 (en) * 2017-04-27 2018-11-01 Regents Of The University Of California Microorganisms and methods for producing cannabinoids and cannabinoid derivatives
CN110892075A (zh) * 2017-07-12 2020-03-17 生物医学股份有限公司 在酵母中生产大麻素
US11035010B2 (en) * 2017-12-14 2021-06-15 Medicinal Genomics Corporation Methods and kits for classifying cannabinoid production in cannabis plants
WO2020069214A2 (en) * 2018-09-26 2020-04-02 Demetrix, Inc. Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives

Also Published As

Publication number Publication date
WO2021055597A1 (en) 2021-03-25
EP4031657A1 (en) 2022-07-27
BR112022004797A2 (pt) 2022-06-21
CN115038786A (zh) 2022-09-09
MX2022003203A (es) 2022-06-08
CA3152803A1 (en) 2021-03-25
IL291328A (he) 2022-05-01
JP2022548904A (ja) 2022-11-22

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