AU2020223370B2 - Enzymes with RuvC domains - Google Patents

Enzymes with RuvC domains Download PDF

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Publication number
AU2020223370B2
AU2020223370B2 AU2020223370A AU2020223370A AU2020223370B2 AU 2020223370 B2 AU2020223370 B2 AU 2020223370B2 AU 2020223370 A AU2020223370 A AU 2020223370A AU 2020223370 A AU2020223370 A AU 2020223370A AU 2020223370 B2 AU2020223370 B2 AU 2020223370B2
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Australia
Prior art keywords
seq
sequence
endonuclease
nos
nuclease system
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AU2020223370A
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AU2020223370A1 (en
Inventor
Lisa ALEXANDER
Christopher Brown
Cristina Butterfield
Audra DEVOTO
Daniela S. A. GOLTSMAN
Rose KANTOR
Jason Liu
Brian Thomas
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Metagenomi Inc
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Metagenomi Inc
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Priority to AU2023206079A priority Critical patent/AU2023206079A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/21Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • CCHEMISTRY; METALLURGY
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2020223370A 2019-02-14 2020-02-14 Enzymes with RuvC domains Active AU2020223370B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023206079A AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962805899P 2019-02-14 2019-02-14
US201962805868P 2019-02-14 2019-02-14
US201962805878P 2019-02-14 2019-02-14
US62/805,899 2019-02-14
US62/805,878 2019-02-14
US62/805,868 2019-02-14
US201962874414P 2019-07-15 2019-07-15
US62/874,414 2019-07-15
PCT/US2020/018432 WO2020168291A1 (en) 2019-02-14 2020-02-14 Enzymes with ruvc domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2023206079A Division AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains

Publications (2)

Publication Number Publication Date
AU2020223370A1 AU2020223370A1 (en) 2021-10-07
AU2020223370B2 true AU2020223370B2 (en) 2023-04-20

Family

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Application Number Title Priority Date Filing Date
AU2020223370A Active AU2020223370B2 (en) 2019-02-14 2020-02-14 Enzymes with RuvC domains
AU2023206079A Pending AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2023206079A Pending AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains

Country Status (9)

Country Link
US (1) US20240117330A1 (ko)
EP (1) EP3924482A4 (ko)
JP (2) JP2022520428A (ko)
KR (2) KR20240007322A (ko)
CN (2) CN113728098A (ko)
AU (2) AU2020223370B2 (ko)
CA (2) CA3241703A1 (ko)
MX (2) MX2021009886A (ko)
WO (1) WO2020168291A1 (ko)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3241703A1 (en) 2019-02-14 2020-08-20 Metagenomi, Inc. Enzymes with ruvc domains
US10982200B2 (en) 2019-02-14 2021-04-20 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
GB2594339B (en) * 2020-02-14 2023-02-08 Metagenomi Inc Enzymes with RUVC domains
KR20240036729A (ko) 2020-03-06 2024-03-20 메타지노미, 인크. 클래스 ii, 타입 v crispr 시스템
AU2021248800A1 (en) 2020-03-31 2022-10-13 Metagenomi, Inc. Class II, Type II CRISPR systems
WO2022056301A1 (en) * 2020-09-11 2022-03-17 Metagenomi Ip Technologies, Llc Base editing enzymes
WO2022098681A2 (en) * 2020-11-03 2022-05-12 Caspr Biotech Corporation Novel class 2 crispr-cas rna-guided endonucleases
BR112023014719A2 (pt) * 2021-01-22 2023-12-05 Metagenomi Inc Novas nucleases quiméricas e manipuladas
WO2022159742A1 (en) * 2021-01-22 2022-07-28 Metagenomi, Inc Novel engineered and chimeric nucleases
CA3214222A1 (en) * 2021-04-30 2022-11-03 Brian Thomas Enzymes with ruvc domains
WO2023122663A2 (en) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Effector proteins and methods of use
WO2023194359A1 (en) * 2022-04-04 2023-10-12 Alia Therapeutics Srl Compositions and methods for treatment of usher syndrome type 2a
WO2023245141A2 (en) * 2022-06-15 2023-12-21 Beam Therapeutics Inc. Compositions and methods for reducing complement activation
WO2024056880A2 (en) * 2022-09-16 2024-03-21 Alia Therapeutics Srl Enqp type ii cas proteins and applications thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013359212B2 (en) * 2012-12-12 2017-01-19 Massachusetts Institute Of Technology Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
EP3303634B1 (en) * 2015-06-03 2023-08-30 The Regents of The University of California Cas9 variants and methods of use thereof
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
WO2018035250A1 (en) * 2016-08-17 2018-02-22 The Broad Institute, Inc. Methods for identifying class 2 crispr-cas systems
WO2019165168A1 (en) * 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
CA3241703A1 (en) 2019-02-14 2020-08-20 Metagenomi, Inc. Enzymes with ruvc domains

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JINEK, M. et al, "A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity", Science, 2012, Vol. 337, pp 816-821 *
SCHMAKOV, S. et al., "Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems", Molecular Cell, 2015, Vol. 60, pp 385-397 *

Also Published As

Publication number Publication date
JP2023179468A (ja) 2023-12-19
JP2022520428A (ja) 2022-03-30
CN116515797A (zh) 2023-08-01
US20240117330A1 (en) 2024-04-11
CA3130135A1 (en) 2020-08-20
AU2020223370A1 (en) 2021-10-07
JP7502537B2 (ja) 2024-06-18
AU2023206079A1 (en) 2023-08-10
EP3924482A4 (en) 2023-04-05
WO2020168291A1 (en) 2020-08-20
MX2021009886A (es) 2021-10-13
MX2023006575A (es) 2023-06-16
CA3241703A1 (en) 2020-08-20
KR102623312B1 (ko) 2024-01-09
EP3924482A1 (en) 2021-12-22
KR20240007322A (ko) 2024-01-16
KR20210139254A (ko) 2021-11-22
CN113728098A (zh) 2021-11-30

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