AU2020104259A4 - Ultrafast dynamics imaging method and system based on single-molecule quantum coherence - Google Patents
Ultrafast dynamics imaging method and system based on single-molecule quantum coherence Download PDFInfo
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Abstract
The present disclosure provides an ultrafast dynamics imaging method and
system based on single-molecule quantum coherence, which belongs to the cross
field of quantum optics and medical science. An ultrafast dynamics imaging
apparatus based on single-molecule quantum coherence includes four parts:
preparation and control of a single molecule coherent state, femtosecond laser
delay lock, excitation of a single molecule and fluorescence detection, and
ultrafast dynamics imaging data extraction and processing. A relative delay of two
laser beams is locked using a differential amplifier and a piezoelectric ceramic by
monitoring an interferometric intensity of a combined laser beam. An electro-optic
modulator is used to periodically change a relative phase of two laser beams to
achieve the modulation of a population probability of a single molecule
excitation state. A coherence factor is calculated to implement ultrafast dynamics
imaging based on single-molecule quantum coherence.
1/4
104
126
128
106 107 129 -- -----
105
112 108--
125
11 1 10915 117 1
1214
118 124
113 +- 2 :122
116 -.-- 120 31 1
101 102 103
- Laser light - Fluorescent emission - ---- Signal cable
FIG. 1
Description
1/4
104
126 128
106 107 129 -- ----- 105 112 108--
125
11 1 10915 117 1 1214 118 124
113 +- 2 :122
116 -.-- 120 1 31
101 102 103 - Laser light - Fluorescent emission - ---- Signal cable
FIG. 1
[0001] The present application relates to an ultrafast dynamics imaging method and system, and in particular, to an ultrafast dynamics imaging method and system based on single-molecule quantum coherence, which belongs to the cross field of quantum optics and medical science.
[0002] Optical Coherence Tomography (OCT) technology has the advantages of non contact, non-damage, and clear imaging, and has been widely used in clinical medical diagnosis. For example, the OCT may be used for the diagnosis of early soft tissue canceration and mediation of brain surgery. Diffuse Optical Tomography has become one of the important methods for breast cancer screening, brain imaging, and soft tissue endoscopy. Compared with these methods, single-molecule fluorescent imaging has characteristics of higher spatial resolution, biocompatibility, operation convenience, etc., and may be used to research life activity processes at a subcellular level, detect ultrafast dynamics behavior of a femtosecond magnitude, and achieve high sensitivity on the order of single molecules. As a result, the single-molecule fluorescent imaging is increasingly used in tumor diagnosis, protein detection, heavy metal ion detection, and new drug development, etc.
[0003] Traditional single-molecule fluorescent imaging is implemented by collecting a number of photons emitted by labeled fluorescent molecules within a certain period of time (usually on the order of milliseconds) from a focus area. Therefore, this method may reflect only the behavior of single-molecule averaged motion dynamics (above milliseconds), impossibly measuring the influence of the life activity processes on the behavior of single-molecule ultrafast dynamics. In particular, recent studies have shown that the life activity processes such as canceration and cell apoptosis will significantly change the behavior of ultrafast decoherence of labeled single molecules. Therefore, a new method is urgently required to detect the behavior of ultrafast decoherence of single molecules so as to understand these life activity processes at a deeper level and provide new methods for canceration diagnosis and health monitoring.
[0004] Any discussion of the prior art throughout the specification should in no way be considered as an admission that such prior art is widely known or forms part of common general knowledge in the field. SUMMARY
[0005] An object of the present invention to provide an improved ultrafast imaging system and method. Preferably this includes resolving the problem that ultrafast dynamics behavior based on single-molecule quantum coherence cannot be implemented by a traditional single-molecule fluorescent imaging, and to propose an ultrafast dynamics imaging method and apparatus based on single-molecule quantum coherence, which utilizes the quantum coherence effect of interaction between femtosecond ultrafast laser and a single molecule to enable ultrafast dynamics imaging at a subcellular level.
[0006] One or more aspects of the present disclosure are achieved through the following technical solutions.
[0007] In one aspect, an ultrafast dynamics imaging apparatus based on single molecule quantum coherence is provided, which includes four parts: preparation and control of a single-molecule coherent state, femtosecond laser delay lock, excitation of a single molecule and fluorescence detection, and ultrafast dynamics imaging data extraction and processing.
[0008] The part of preparation and control of a single molecule coherent state includes a femtosecond laser, a polarizer, an equal ratio beam splitter, an electro-optic modulator, an electro-optic modulator controller, a corner cube retroreflector 1, and a corner cube retroreflector 2.
[0009] The femtosecond laser is used to prepare a single molecule coherent state, excite, and obtain single-molecule fluorescent emission.
[0010] The polarizer is used to generate horizontally polarized laser light.
[0011] The equal ratio beam splitter is used to generate two laser beams with equal light intensity. A first laser beam, after being reflected by the corner cube retroreflector 1, returns to the equal ratio beam splitter. A second laser beam, after being reflected by the corner cube retroreflector 2 through the electro-optic modulator, returns to the equal ratio beam splitter. The two laser beams are recombined at the equal ratio beam splitter.
[0012] The electro-optic modulator controller is used to apply a voltage with a specific waveform and period to the electro-optic modulator, and change a relative phase Aq of two femtosecond laser beams.
[0013] The corner cube retroreflector 1 is located in a transmission direction of the equal ratio beam splitter. The electro-optic modulator and the corner cube retroreflector 2 are located in a reflection direction of the equal ratio beam splitter.
[0014] Two femtosecond laser beams are used to prepare a single molecule coherent state. Periodic phase modulation is used to modulate the population probability of the excited state of a single molecule, to realize periodic modulation of the single molecule coherent state and its fluorescent emission intensity, suppress background noises and extract single-molecule ultrafast decoherence parameters.
[0015] The part of the femtosecond laser delay lock includes a piezoelectric ceramics, an unequal ratio beam splitter, a high-speed photoelectric detector, a differential amplifier, and a piezoelectric ceramic controller.
[0016] The piezoelectric ceramic is fixed on a back face of the corner cube retroreflector 1 and used to change the optical path of the first laser beam, then change the relative delay AT of the two laser beams. A specific value of the relative delay is determined by a voltage output from the piezoelectric ceramic controller.
[0017] The unequal ratio beam splitter is used to split the combined laser beam, where a weaker part is reflected into the high-speed photoelectric detector, and a stronger part is transmitted into a dichroic mirror.
[0018] In order to lock the relative delay AT, the combined laser beam is reflected by the unequal ratio beam splitter and enters the high-speed photoelectric detector to obtain a laser interferometric light intensity. When the relative delay AT changes, the laser interferometric light intensity will change correspondingly. A light intensity signal is input to the differential amplifier and compared with a reference voltage. A comparison signal is input to the piezoelectric ceramic controller. The piezoelectric ceramic controller changes the position of the piezoelectric ceramic according to positive and negative values of an input voltage to achieve the locking of the delay AT.
[0019] The part of excitation of a single molecule and fluorescence detection includes a dichroic mirror, an objective lens, a to-be-tested single-molecule sample, a color filter combination, a polarization beam splitter, a time-resolved camera 1 and a time resolved camera 2.
[0020] The combined laser beam, after being transmitted by the unequal ratio beam splitter, is reflected by the dichroic mirror and focused by the objective lens to excite the single-molecule sample.
[0021] The fluorescent light emitted by a single molecule, after passing through the objective lens and the dichroic mirror, is filtered by the color filter combination to remove laser light and background noise photons. The color filter combination is used to filter out the influence of laser light and a background photon. The filtered fluorescent light, after being split by the polarization beam splitter, becomes horizontal and vertical fluorescent light, which are detected by the time-resolved camera 1 and the time-resolved camera 2, respectively.
[0022] The part of ultrafast dynamics imaging data extraction and processing is composed of a computer and corresponding data processing programs.
[0023] The principle of the technical solutions of the present invention is as follows:
[0024] According to the quantum effect of interaction between the femtosecond ultrafast laser and a single molecule, the population probability of a single molecule excitation state will oscillate as a relative delay AT of a first femtosecond laser beam and a second femtosecond laser beam changes. Due to the vibrational relaxation of the single molecule and the interaction between an ambient environment and the single molecule, the oscillation exhibits obvious damping behavior. That is, the longer the delay time AT is, the smaller the change in population probability is. The process of detecting the oscillation may characterize the behavior of single-molecule ultrafast decoherence, and obtain parameters such as coherence time. Limited by the weak fluorescent light emission ability of the single molecule, an oscillation damping signal is often submerged with noises. In order to suppress the noises, according to the present disclosure, while the piezoelectric ceramic is used to change the relative delay AT of the first femtosecond laser beam and the second femtosecond laser beam, the electro-optic modulator is used to periodically change the relative phase Ap of two femtosecond pulses to achieve the periodic modulation of the population probability of the single-molecule excitation state. After modulation and demodulation processes, modulation magnitude is extracted, but the background noise is not modulated, so that the influence of the background noise may be significantly suppressed. The coherence factor obtained from data processing may characterize the population probability of the single molecule under the delay and the ultrafast decoherence process. By changing the delay time AT, ultrafast dynamics imaging under different delays may be obtained. By analyzing the ultrafast dynamics imaging, the ultrafast decoherence behavior of an entire research system may be obtained. By calculating the association characteristic of the ultrafast decoherence behavior in local areas, the influence of an environment on the behavior of single-molecule ultrafast decoherence may be further clarified.
[0025] FIG. 1 is a schematic diagram illustrating an ultrafast dynamics imaging apparatus based on single-molecule quantum coherence.
[0026] FIG. 2 is a schematic diagram illustrating the principle of an ultrafast dynamics imaging method based on single-molecule quantum coherence.
[0027] FIG. 3 is a schematic diagram illustrating a time-resolved camera.
[0028] FIG. 4 shows the change of femtosecond interferometric intensity over time before and after the relative delay of two laser beams is locked.
[0029] FIG. 5 shows the ultrafast dynamics imaging of Lung cancer cell line A549.
[0030] Preferred embodiments of present invention will be further described below in conjunction with the drawings and embodiments.
[0031] As shown in FIG. 1, an ultrafast dynamics imaging apparatus based on single molecule quantum coherence includes four parts: preparation and control of a single molecule coherent state (101), femtosecond laser delay lock (102), excitation of a single molecule and fluorescence detection (103) and ultrafast dynamics imaging data extraction and processing (104).
[0032] The part of preparation and control of a single molecule coherent state (101) includes a femtosecond laser (105), a polarizer (106), a reflector (107), an equal ratio beam splitter (108), an electro-optic modulator (112), an electro-optic modulator controller (113), a corner cube retroreflector 1 (110) and a corner cube retroreflector 2 (114).
[0033] The femtosecond laser (105) is used to prepare a single molecule coherent state, excite a single molecule and obtain fluorescent light. A center wavelength of the femtosecond laser needs to be adjusted according to the excited single molecule. A pulse width of the femtosecond laser should be adjusted according to an interaction intensity of laser light and a single molecule. In an example, a femtosecond laser with a center wavelength of 625 nm, a pulse width of 150 fs, and a repetition frequency of 80 MHz is used in the embodiments.
[0034] The reflector (107) is used to change laser directions.
[0035] The equal ratio beam splitter (108) is used to generate two laser beams with completely equal light intensity. A first laser beam (109), after being reflected by the corner cube retroreflector 1 (110), returns to the equal ratio beam splitter (108). A second laser beam (111), after being reflected by the corner cube retroreflector 2 (114) through the electro-optic modulator (112), returns to the equal ratio beam splitter (108). The two laser beams are combined at an equal ratio beam splitter (108) to form a combined laser beam (115).
[0036] The electro-optic modulator controller (113), after applying a voltage with a specific waveform and period to the electro-optic modulator (112), may change a relative phase Ap (205) of the two laser beams. In some examples, a sawtooth waveform is used, a phase adjustment range is 0-22, and a modulation frequency is set to 1 kHz.
[0037] Two femtosecond laser beams are used to prepare a single molecule coherent state. Periodic phase modulation is used to modulate a population probability of a single molecule laser state to realize periodic modulation of the single-molecule coherent state and an emitted fluorescent emission intensity, suppress background noises, and extract single-molecule ultrafast decoherence parameters.
[0038] The part of femtosecond laser delay lock (102) includes a piezoelectric ceramics (116), an unequal ratio beam splitter (117), a high-speed photoelectric detector (118), a differential amplifier (119), and a piezoelectric ceramic controller (120).
[0039] The piezoelectric ceramic (116) is fixed on the back face of the corner cube retroreflector 1 (110). The piezoelectric ceramic (116) is a precise displacement apparatus for changing an optical path of the first laser beam (109), and then changing the relative delay AT (204) of the two laser beams. A specific value of the relative delay AT (204) is determined by a voltage output from the piezoelectric ceramic controller (120).
[0040] The unequal ratio beam splitter (117) is used to split the combined laser beam (115), where a weaker part is reflected into the high-speed photoelectric detector (118), and a stronger part is transmitted into a dichroic mirror (121). In some examples, the splitting ratio of the unequal ratio beam splitter (117) is 1:9, in which 1/10 of laser light is reflected, and 9/10 of laser light is transmitted.
[0041] Due to factors such as mechanical peristalsis and vibration, the relative delay AT (204) of the two laser beams will jitter regularly or randomly, leading to a decrease in an imaging signal-to-noise ratio and even a failure to obtain ultrafast dynamics imaging. In order to lock the relative delay AT (204), the combined laser beam (115) is reflected by the 1:9 beam splitter (117). 1/10 of light intensity enters the high-speed photoelectric detector (118), which converts laser interferometric light intensity into a voltage value. When the relative delay AT (204) changes, the laser interferometric light intensity will change correspondingly (FIG. 4a), and thereby a photovoltage value will change therewith. The obtained photovoltage is input into the differential amplifier (119) and compared with a reference voltage. When the photovoltage is greater than the reference voltage, the piezoelectric ceramic controller (120) outputs a positive voltage, and the piezoelectric ceramic (116) increases the optical path of the first laser beam (109). When the photovoltage is less than the reference voltage, the piezoelectric ceramic controller (120) outputs a negative voltage, and the piezoelectric ceramic (116) reduces the optical path of the first laser beam (109).
[0042] FIG. 4 shows a photovoltage signal obtained by the high-speed photoelectric detector (118) with the relative delay AT (204) of the laser beams being unlocked. The photovoltage signal changes irregularly over time, indicating that the relative delay AT (204) changes irregularly. FIG. 4 also shows a photovoltage signal obtained by the high-speed photoelectric detector (118) with the relative delay AT (204) of the laser beams being locked. The photovoltage signal is stable, which indicates that the relative delay AT (204) is very stable.
[0043] The part of single molecule excitation and fluorescence detection (103) includes a dichroic mirror (121), an objective lens (122), a to-be-tested single molecule sample (123), a color filter combination (125), a polarization beam splitter (126), a time-resolved camera 1 (127) and a time-resolved camera 2 (128).
[0044] The dichroic mirror (121) is used to reflect a combined laser beam (115) and transmit single-molecule fluorescent light (124). The type of the dichroic mirror (121) may vary according to a center wavelength of femtosecond laser light and a wavelength of single-molecule fluorescent light. In some examples, a model of the dichroic mirror used is ET6551p, of which the transmittance for laser light above 655 nm is more than 99%, and the transmittance for laser light below 648 nm is less than 0.001%.
[0045] The combined laser beam (115) is reflected by the dichroic mirror (121) and focused by the objective lens (122) to excite the single molecule sample (123). The objective lens is used to focus laser light, and a wide field mode is adopted. In some examples, the objective lens used is a Nikon commercial objective lens with a numerical aperture of 1.3. The single molecule sample may be either a naked single molecule spin-coated on a glass slide or a fluorescent molecule labeled in a biological body such as a cell. In some examples, the sample used is a squaraine-derived rotaxane molecule (referred to as SR molecule for short) labeled in a lung cancer cell A549. A maximum absorption wavelength of the molecule is at 620 nm, and a maximum fluorescent light emission peak is at 670 nm.
[0046] The fluorescent light (124) emitted by a single molecule, after passing through the objective lens (122) and the dichroic mirror (121), is filtered by the color filter combination (125). The color filter combination is used to filter out the influence of laser light and a background photon pair. In an example, a notch filter and a long-pass fluorescent light filter are selected in the embodiments. The filtered fluorescent light, after being split by the polarization beam splitter (126), has horizontal and vertical fluorescent light intensities, which are detected by the time-resolved camera 1 (127) and the time-resolved camera 2 (128) respectively.
[0047] The time-resolved cameras are used to detect fluorescent light intensities, and have a time resolving capability, so that an absolute arrival time of each fluorescent photon may be given. In an example, a PF32 time-resolved camera produced by Photon Force Ltd. is used in the embodiments, and a time resolution thereof is 55 picoseconds.
[0048] The part of ultrafast dynamics imaging data extraction and processing (104) is composed of a computer (127) and corresponding data processing programs. The specific process includes:
[0049] 1) selecting a delay time AT and an integration time T;
[0050] 2) calculating sums of fluorescent photons collected by each pixel (301) on the time-resolved camera 1 (125) and the time-resolved camera 2 (126) within the
integration time T to obtain fluorescent light intensities, which are I and I2 respectively;
[0051]3) performing, by the time-resolved camera 1 (125) and the time-resolved camera 2 (126), fast Fourier transform on an arrival time of each fluorescent photon
within the integration time T to obtain modulation intensities M1X1'I and Mx2y2 at a modulation frequency;
[0052] 4) calculating a coherence factor 7 of each pixel by the following formula: y=_ +xy2X M ' xly+ Mx2y( 2y2 x1Y1 xly1 x2y2 12 1 I, 1 2
[0053] 5) wherein subscripts 1, 2 respectively represent the time-resolved camera 1 (125) and the time-resolved camera 2 (126); xl, yl, and x2, y2 respectively represent serial numbers (302, 303) of pixels on the time-resolved camera 1 (125) and the time resolved camera 2 (126) in x and y directions; x and y are serial numbers of final ultrafast dynamics imaging pixels.
[0054] FIG. 2 is a schematic diagram illustrating the principle of ultrafast dynamics imaging based on single-molecule quantum coherence. Steps of obtaining the ultrafast dynamics imaging based on single-molecule quantum coherence is described below in conjunction with this drawing.
[0055] 1) calibrating relative delay of laser beams to zero: turning on a laser (105), outputting a sawtooth voltage by a piezoelectric ceramic controller (120), continuously changing an optical path of a first laser beam (109), monitoring an interferometric light intensity through a high-speed photoelectric detector (118), wherein when the interferometric light intensity reaches a maximum value, relative delay AT (204) of two laser beams is zero, and fixing a zero point voltage of the piezoelectric ceramic controller (120);
[0056] 2) changing the voltage output from the piezoelectric ceramic controller (120), fixing the relative delay of the two laser beams to be ATI, and locking the delay through a laser delay locking system (102);
[0057] 3) outputting the sawtooth voltage to an electro-optic modulator (112) by an electro-optic modulator controller (113), periodically changing a relative phase Ap (205) of the two laser beams, and periodically modulating a population probability of a single molecule excitation state;
[0058] 4) fixing a lung cancer cell A549 with an SR molecule labeled under an objective lens, and adjusting the position of the objective lens to focus;
[0059]5) using a combined laser beam (115) to excite single molecules and obtain fluorescent light (124), and collecting single-molecule fluorescent light through a time-resolved camera 1 (126) and a time-resolved camera 2 (127), wherein the collection time is an integration time, which may be Is in some examples;
[0060]6) calculating a coherence factor for each pixel according to the above data processing to implement ultrafast dynamics imaging (206) under the relative delay ATI;
[0061] 7) changing the voltage output from the piezoelectric ceramic controller (120), fixing the relative delay of the two laser beams to be AT2, and repeating the steps 3-6 to implement ultrafast dynamics imaging (207) under this delay;
[0062] 8) repeating the above steps to implement ultrafast dynamics imaging (206 208) based on single-molecule quantum coherence.
[0063] FIG. 5 shows ultrafast dynamics imaging of a lung cancer cell A549 with relative delays being 0 fs (a), 50 fs (b), 100 fs (c), and 150 fs (d).
[0064] Other embodiments of the present disclosure will be readily apparent to those skilled in the art after considering the specification and practicing the contents disclosed herein. The present application is intended to cover any variations, uses, or adaptations of the present disclosure, which follow the general principle of the present disclosure and include common knowledge or conventional technical means in the art that are not disclosed in the present disclosure. The specification and examples are to be regarded as illustrative only. The true scope and spirit of the present disclosure are pointed out by the following claims.
[0065] The above are only examples of the present application and not intended to limit this application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the application.
[0066] Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is to say, in the sense of "including, but not limited to".
Claims (6)
1. An ultrafast dynamics imaging system based on single-molecule quantum coherence, comprising four parts: preparation and control of a single molecule coherent state (101), femtosecond laser delay lock (102), excitation of a single molecule and fluorescence detection (103), and ultrafast dynamics imaging data extraction and processing (104).
2. The ultrafast dynamics imaging system based on single-molecule quantum coherence according to claim 1, wherein relative delay AT (204) of two laser beams is locked using a differential amplifier (119), a piezoelectric ceramic controller (120), and a piezoelectric ceramic (116) by monitoring a interferometric intensity of a combined laser beam (115).
3. The ultrafast dynamics imaging system based on single-molecule quantum coherence according to claim 1, wherein a piezoelectric ceramic (116) is fixed on a back face of a corner cube retroreflector 1 (110).
4. The ultrafast dynamics imaging system based on single-molecule quantum coherence according to claim 1, wherein single-molecule fluorescent light is collected using a time-resolved camera 1 (127) and a time-resolved camera 2 (128); the time resolved camera 1 (127) and the time-resolved camera 2 (128) detect a horizontal component and a vertical component of the single-molecule fluorescent light (124) respectively.
5. The ultrafast dynamics imaging system based on single-molecule quantum coherence according to claim 1, wherein ultrafast dynamics imaging based on a coherence factor (x is implemented; the coherence factor (' is calculated by the following formula: xly1 x2y2 M lyl +MxN'
~~ 1x2y2 1'1 +jxlyl xy1 X xx1Y1 + 2 1 2y Xx2y2 j(1 /
= 1)Y 2 1(M wherein I' and I2 are sums of fluorescent photons collected by each pixel on a
time-resolved camera 1 (125) and a time-resolved camera 2 (126) within an
integration time;M and M2y2are modulation intensities obtained by the time
resolved camera 1 (125) and the time-resolved camera 2 (126) performing fast Fourier transform on arrival times of the fluorescent photons within an integration time T; x and y are serial numbers of imaging pixels.
6. An ultrafast dynamics imaging method based on single-molecule quantum coherence, comprising steps of: 1) calibrating relative delay of laser beams to zero, comprising: turning on a laser (105), outputting a sawtooth voltage by a piezoelectric ceramic controller (120), continuously changing an optical path of a first laser beam (109), monitoring an interferometric light intensity through a high-speed photoelectric detector (118), wherein when the interferometric light intensity reaches a maximum value, relative delay AT (204) of two laser beams is zero, and fixing a zero point voltage of the piezoelectric ceramic controller (120); 2) changing the voltage output from the piezoelectric ceramic controller (120), fixing the relative delay of the two laser beams to be AT1, and locking the delay through a laser delay locking system (102); 3) outputting the sawtooth voltage to an electro-optic modulator (112) by an electro optic modulator controller (113), periodically changing a relative phase Ap (205) of the two laser beams, and periodically modulating a population probability of a single molecule excitation state; 4) fixing a sample with a fluorescent molecule labeled under an objective lens, and adjusting a position of the objective lens to focus; ) using a combined laser beam (115) to excite single molecules and obtain fluorescent light (124), and collecting single-molecule fluorescent light through a time-resolved camera 1 (126) and a time-resolved camera 2 (127); 6) calculating a coherence factor for each pixel to implement ultrafast dynamics imaging (206) under the relative delay AT1; 7) changing the voltage output from the piezoelectric ceramic controller (120), fixing the relative delay of the two laser beams to be AT2, and repeating the steps 3) to 6) to implement ultrafast dynamics imaging (207) under this delay; 8) repeating the above steps to implement ultrafast dynamics imaging (206-208) based on single-molecule quantum coherence.
PAR2027739AU 1/4 23 Dec 2020 2020104259
FIG. 1
PAR2027739AU 2/4 23 Dec 2020 2020104259
FIG. 2
PAR2027739AU 3/4 23 Dec 2020 2020104259
FIG. 3
PAR2027739AU 4/4 23 Dec 2020 2020104259
FIG. 4
FIG. 5
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| CN107478630B (en) * | 2017-09-12 | 2020-04-17 | 山西大学 | Device and method for improving single-molecule optical imaging contrast |
| CN109632726B (en) * | 2018-12-13 | 2020-10-23 | 中山大学 | Molecular dynamics measurement method and device based on quantum coherent control |
| CN111307756A (en) * | 2019-11-20 | 2020-06-19 | 南京航空航天大学 | Frequency-adjustable ultrafast time resolution transient reflection spectrometer |
| CN111308489B (en) * | 2020-03-16 | 2022-02-11 | 杭州爱莱达科技有限公司 | Optical domain analysis focusing method and device of pseudo-random code phase modulation laser radar |
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2020
- 2020-12-07 CN CN202011417542.0A patent/CN112595699B/en active Active
- 2020-12-23 AU AU2020104259A patent/AU2020104259A4/en active Active
Cited By (2)
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| CN117503203A (en) * | 2024-01-03 | 2024-02-06 | 之江实验室 | Phase aberration correction method and system for ultrasonic ring array imaging |
| CN117503203B (en) * | 2024-01-03 | 2024-03-22 | 之江实验室 | Phase aberration correction method and system for ultrasonic ring array imaging |
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