AU2020102196A4 - Method for detecting growth state of nucleated pearls in living pearl oysters - Google Patents

Method for detecting growth state of nucleated pearls in living pearl oysters Download PDF

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AU2020102196A4
AU2020102196A4 AU2020102196A AU2020102196A AU2020102196A4 AU 2020102196 A4 AU2020102196 A4 AU 2020102196A4 AU 2020102196 A AU2020102196 A AU 2020102196A AU 2020102196 A AU2020102196 A AU 2020102196A AU 2020102196 A4 AU2020102196 A4 AU 2020102196A4
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pearl
image
nacre
nucleus
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Zhiyi Bai
Quanzhi Bao
Jiale Li
Minglong Sun
Tianyang Sun
Li Yuan
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Shanghai Ocean University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • A01K61/56Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B15/00Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B15/00Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons
    • G01B15/02Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons for measuring thickness
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B15/00Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons
    • G01B15/04Measuring arrangements characterised by the use of electromagnetic waves or particle radiation, e.g. by the use of microwaves, X-rays, gamma rays or electrons for measuring contours or curvatures
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/02Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
    • G01N23/04Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/02Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
    • G01N23/06Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and measuring the absorption
    • G01N23/083Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and measuring the absorption the radiation being X-rays
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Electromagnetism (AREA)
  • Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
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  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Toxicology (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Image Analysis (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)

Abstract

OF THE DISCLOSURE Disclosed is a method for detecting the growth status of nucleated pearls in living pearl oysters, comprising: irradiating a standard sphere with X rays to obtain an initial image with a radius of ro, then identifying the boundary points of the initial image and fitting to obtain the fitted circle of the standard sphere with a radius of Ro, calculating the scale factor =ro/Ro; performing the same method to obtain the maximum diameter dmax and minimum diameter dmi of the pearl image to be detected, the radius R1 of the nacre fitted circle, and the radius R2 of the nucleus fitted circle, the nucleus-inserted pearl radius ri= Ri and the nucleus radius r2=pR2; and accordingly calculating the nucleus-inserted pearl size d=2ri, the nacre thickness r=ri-r2 and the pearl roundness DDP=(dmax-dmi)/dmaxx100 %. The invention solves the problem that the traditional sampling and detection of pearl oysters cannot detect and eliminate pearl oysters in a poorly-growing state in time, and can significantly improve the quality of pearls under cultivation conditions through timely tracking and detection. 11

Description

METHOD FOR DETECTING GROWTH STATE OF NUCLEATED PEARLS IN LIVING PEARL OYSTERS BACKGROUND OF THE INVENTION
[0001] 1. Technical Field
[0002] The invention belongs to the technical field of aquaculture detection, and
specifically relates to a method for detecting the growth state of nucleated pearls in living
pearl oysters, where an X-ray radiation source is used to track and detect the growth of
nucleated pearls in the living pearl oysters.
[0003] 2. Description of Related Art
[0004] At present, the growth status of pearls is generally obtained by sampling a
batch of cultured pearl oysters, and then dissecting the pearl oysters to detect the
growth status of the pearls. Such a detection method can assess the culture status of pearl
oysters in the same batch of cultured groups and guide the management of aquaculture
production, but it cannot detect and eliminate the pearl oysters in a poorly-growing state in
time. Due to the long cultivation cycle of pearls, the cultivation of pearl oysters in a
poorly-growing state will increase the cultivation cost and decrease the cultivation
efficiency.
[0005] X-ray detection is convenient and practical. Under certain dose conditions,
it can achieve unique detection effects that other detection methods cannot achieve,
without damaging a detected object. The images can be visually analyzed or archived for
future reference. In clinic, it is often used for bone imaging. As a simple and commonly
used inspection method, it can observe the characteristics of bone surface morphology
from different angles. The penetrating power of X rays is related to the density of the
substance. The property of differential absorption can be used to distinguish substances
with different densities, so that the surface thickness of the detected object can be clearly detected.
BRIEF SUMMARY OF THE INVENTION
[0006] In order to overcome the problem that the traditional pearl oyster sampling
test cannot detect and eliminate pearl oysters in a poorly-growing state in time, the
objective of the invention is to provide a method for detecting the growth state of
nucleated pearls in living pearl oysters, which can track and detect the growth state and
grade of the nucleus-inserted pearls in the living pearl oysters, such as the size, the nacre
thickness, and the roundness.
[0007] In order to solve the above technical problems, the technical solution
adopted by the invention is as follows:
[0008] The invention provides a method for detecting the growth state of nucleated
pearls in living pearl oysters. The detection of nucleated pearls in the living pearl oysters
using X rays includes the following steps:
[0009] (1) adjusting the target current of an X-ray tube to 0.5mA, placing a
standard sphere between an X-ray radiation source and an imaging plane, keeping the
center of the standard sphere on a reference straight line, and enabling the X-ray radiation
source to irradiate the standard sphere to obtain an initial image of the standard sphere on
the imaging plane, and measuring the radius of the initial image as ro;
[0010] (2) identifying the boundary points of the initial image of the standard
sphere in Step (1), fitting to obtain a fitted circle of the standard sphere, measuring the
radius Ro of the fitted circle, and calculating the scale coefficient =ro/Ro;
[0011] (3) placing a nucleus-inserted pearl of a living pearl oyster between the
X-ray radiation source and the imaging device, keeping the center of the nucleus wrapped
in the annular nacre on the reference straight line, and enabling the X-ray radiation source
to irradiate the nacre and pearl nucleus to obtain a nacre image and a nucleus image on the imaging plane, and measuring the maximum diameter dmax and minimum diameter dmin of the obtained pearl image;
[0012] (4) identifying the boundary points of the nacre image and the nucleus
image in Step (3) respectively, and fitting to obtain an outermost nacre fitted circle and a
central nucleus fitted circle, and measuring the radii of the nacre fitted circle and the
nucleus fitted circle as R1 and R2 , respectively, and calculating the radius of the
nucleus-inserted pearl as ri= Ri and the radius of the nucleus as r2=pR2;
[0013] (5) based on the dmax, dmin, ri and r2, and according to the following
calculation formulas, calculating the size d, the nacre thickness r and the pearl roundness
DDP of the nucleated pearl in living pearl oyster, wherein:
[0014] the pearl size d=2ri;
[0015] the nacre thickness r=r-r2;
[0016] the pearl roundness DDP=(dmax-dmin)/dmaxX100%.
[0017] According to some embodiments of the invention, the standard sphere is a
lead ball with a diameter of lcm, which can completely block the transmission of X-rays
and can be used as a standard sphere for measurement.
[0018] It should be noted that the pearl roundness DDP adopts the diameter
difference percentage, and the measured maximum diameter dmax and minimum diameter
dmin of the pearl image do not need to be calibrated by the scale factor u.
[0019] According to some embodiments of the invention, the method of
identifying the boundary points of the image of the object to be detected is to distinguish
the pixels of the image of the object to be detected according to the gray value of each
pixel: when the gray value of a pixel is higher than a gray threshold, the pixel is a pixel
that constitutes the image of the object to be detected; when the gray value of a pixel is
lower than the gray threshold, the pixel is a pixel that constitutes the background; and in
two adjacent pixels, when a first pixel is a pixel that constitutes the image of the object to be detected and a second pixel is a pixel that constitutes the background, taking the first pixel as a boundary point of the image of the object to be detected, all the pixels are detected one by one to obtain all the boundary points of the image of the object to be detected. According to the above method, the boundary points of the initial image of the standard sphere, the nacre image and the nucleus image can be identified, respectively.
[0020] Compared with the prior art, the invention has the following beneficial
effects:
[0021] 1. The invention uses X-ray imaging technology to detect nucleus-inserted
pearls in the living pearl oysters, and can accurately and nondestructively detect the
growth status (such as the size, nacre thickness, and roundness) of nucleated pearls in the
living pearl oyster without killing the pearl oysters; thus, it does not affect the continued
growth and development of the pearl oysters.
[0022] 2. According to the invention, the error in the calculation process is
controllable, and the measurement results are accurate. It realizes real-time tracking of
breeding conditions of individual pearl oysters during the cultivation process, which can
significantly increase the pearl quality rate of cultivated pearls and is beneficial to pearl
quality evaluation and culture management decision-making under culture conditions.
BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS
[0023] Fig. 1 is a flow chart of the method for detecting the growth of nucleated
pearls in living pearl oysters of the invention.
[0024] Fig. 2 is a schematic diagram of X-ray imaging of a nucleus-inserted pearl
in a Hyriopsis cumingii in an embodiment, dmax and dmin represent the maximum
diameter and minimum diameter of the image of the pearl to be detected, respectively.
[0025] Fig. 3 is a schematic diagram of a fitted image of a nucleus-inserted pearl
in a Hyriopsis cumingii in the embodiment; where: the radius of the initial image is ro which is the radius of the initial image of the standard sphere, Ro is the radius of thefitted image of the standard sphere, and R1 and R2 are the radii of the outermost nacre fitted circle and the central nucleus fitted circle of the pearl to be detected.
DETAILED DESCRIPTION OF THE INVENTION
[0026] The invention will be further described below with reference to the
accompanying drawings and specific embodiments, so that those skilled in the art can
better understand the invention and implement it, but the embodiments are not intended to
limit the invention.
[0027] In the following embodiments, the method for detecting the growth state of
nucleus-inserted pearls in living pearl oysters to be detected is shown in Fig. 1; the method
specifically comprises:
[0028] Si: adjusting the target current of an X-ray tube to 0.5mA, placing a
standard sphere between an X-ray radiation source and an imaging plane, keeping the
center of the standard sphere on a reference straight line, and enabling the X-ray radiation
source to irradiate the standard sphere to obtain an initial image of the standard sphere on
the imaging plane, and measuring the radius of the initial image as ro;
[0029] S2: identifying the boundary points of the initial image of the standard
sphere in Si, fitting to obtain afitted circle of the standard sphere, measuring the radius Ro
of the fitted circle, and calculating the scale coefficient =ro/Ro;
[0030] S3: placing a nucleus-inserted pearl of a living pearl oyster to be detected
between the X-ray radiation source and the imaging device, keeping the center of the
nucleus wrapped in the annular nacre on the reference straight line, and enabling the X-ray
radiation source to irradiate the nacre and pearl nucleus to obtain a nacre image and a
nucleus image on the imaging plane, and measuring the maximum diameter dmax and
minimum diameter dmin of the obtained pearl image;
[0031] S4: identifying the boundary points of the nacre image and the nucleus
image in S3 respectively, and fitting to obtain an outermost nacre fitted circle and a central
nucleus fitted circle, and measuring the radii of the nacre fitted circle and the nucleus
fitted circle as R and R2 , respectively, and calculating the radius of the nucleus-inserted
pearl as ri= Ri and the radius of the nucleus as r2=pR2; and
[0032] S5: based on the dmax, dmin, ri and r2, and according to the following
calculation formulas, calculating the size d, the nacre thickness r and the pearl roundness
DDP of the living pearl oyster to be detected, wherein: the pearl size d=2ri, the nacre
thickness r=ri-r2, and the pearl roundness DDP=(dmax-dmin)/dmaxx100%.
[0033] According to some embodiments of the invention, the method of
identifying the boundary points of the image of the object to be detected is to distinguish
the pixels of the image of the object to be detected according to the gray value of each
pixel: when the gray value of a pixel is higher than a gray threshold, the pixel is a pixel
that constitutes the image of the object to be detected; when the gray value of a pixel is
lower than the gray threshold, the pixel is a pixel that constitutes the background; and in
two adjacent pixels, when a first pixel is a pixel that constitutes the image of the object to
be detected and a second pixel is a pixel that constitutes the background, taking the first
pixel as a boundary point of the image of the object to be detected, all the pixels are
detected one by one to obtain all the boundary points of the image of the object to be
detected; according to the above method, the boundary points of the initial image of the
standard sphere, the nacre image and the nucleus image can be identified, respectively.
[0034] Example 1
[0035] Six mussels of Hyriopsis cumingii with nucleated pearls were randomly
selected. First, the above-mentioned detection method was used to nondestructively detect
the pearl properties in vivo, and then the mussels were dissected to take pearls and the
traditional method was used for destructively testing the pearls. Specific steps were as follows:
[0036] An X-ray radiation source is connected to the electronic system imaging
technology to detect the growth of the nucleus-inserted pearls in the living Hyriopsis
cumingii , including the pearl size, the nacre thickness and the roundness; the method
was the same as above.
[0037] As shown in Fig. 2, the X-ray imaging of the nucleus-inserted pearls in the
living Hyriopsis cumingii measured the maximum and minimum diameters of the pearl
image to be detected as dmax and dmin, respectively.
[0038] As shown in Fig. 3, according to the radius ro of the initial image of the
standard sphere and the radius Ro of the fitted image of the standard sphere, the radii R
and R2 of the outermost nacre fitted circle and the central nucleus fitted circle of the of the
nucleus-inserted pearl in the living Hyriopsis cumingii were obtained, and the
parameters were then calculated according to the formulas.
[0039] The traditional method refers to the GB/T18781-2008 national standard
"Pearl Classification" measurement method. The pearls were taken from the living
mussels, and then the maximum diameter (dmax) and minimum diameter (dmin) of the
pearls were measured with a vernier caliper, and then the size and roundness of the pearls
were calculated. The thickness of the nacre was detected in the same way as this method.
The detection results of the two methods are shown in Table 1. The consistency of the
three indicators is more than 95%, indicating that the detection method of the invention is
reliable for in vivo detection.
[0040] Table 1
The method for detecting the growth state Traditional detection method by of nucleus-inserted pearls in living pearl dissecting mussels to take pearls Pearl oysters of the invention Nacre Pearl Nacre Pearl thickness Roundness size(mm) thickness Roundness size(mm) (mm) (mm) 1 12.56 1.61 85.1% 13.01 1.69 86%
2 13.42 1.66 75% 12.95 1.61 78.9% 3 11.43 1.24 80% 11.71 1.3 82.1% 4 12.11 1.58 81% 11.96 1.61 78.8% 5 10.86 1.11 75.9% 11.42 1.07 74% 6 12.01 1.29 71.9% 12.42 1.32 74.2%
[0041] The foregoing description of the embodiments is to facilitate those of
ordinary skill in the art to understand and use the invention. Those skilled in the art can
obviously make various modifications to these embodiments and apply the general
principles described here to other embodiments without creative work. Therefore, the
invention is not limited to the above-mentioned embodiment. Improvements and
modifications made by those skilled in the art based on the principles of the invention
without departing from the scope of the invention should fall within the protection scope
of the invention.

Claims (4)

What is claimed is:
1. A method for detecting the growth state of nucleated pearls in living pearl oysters,
wherein the method adopts X rays to detect nucleated pearls in the living pearl oyster, and
the method comprises the following steps:
(1) adjusting the target current of an X-ray tube to 0.5mA, placing a standard sphere
between an X-ray radiation source and an imaging plane, keeping the center of the
standard sphere on a reference straight line, and enabling the X-ray radiation source to
irradiate the standard sphere to obtain an initial image of the standard sphere on the
imaging plane, and measuring the radius of the initial image as ro;
(2) identifying boundary points of the initial image of the standard sphere in Step (1),
fitting to obtain a fitted circle of the standard sphere, measuring the radius Ro of the fitted
circle, and calculating the scale coefficient [=ro/Ro;
(3) placing a nucleus-inserted pearl of a living pearl oyster between the X-ray radiation
source and the imaging device, keeping the center of the nucleus wrapped in the annular
nacre on the reference straight line, and enabling the X-ray radiation source to irradiate the
nacre and the pearl nucleus to obtain a nacre image and a nucleus image on the imaging
plane, and measuring the maximum diameter dmax and minimum diameter dmin of the
obtained pearl image;
(4) identifying the boundary points of the nacre image and the nucleus image in Step
(3) respectively, and fitting to obtain an outermost nacre fitted circle and a central nucleus
fitted circle, and measuring the radii of the nacre fitted circle and the nucleus fitted circle
as R1 and R2 , respectively, and calculating the radius of the nucleus-inserted pearl as
ri= Ri and the radius of the nucleus as r2=pR2; and
(5) based on the dmax, dmin, ri and r2, and according to the following calculation
formulas, calculating the size d, the nacre thickness r and the pearl roundness DDP of the
nucleated pearl in living pearl oyster, wherein: the pearl size d=2ri; the nacre thickness r=ri-r2; the pearl roundness DDP=(dmax-dmin)/dmaxx100%.
2. The method for detecting the growth state of nucleated pearls in living pearl oysters
according to Claim 1, wherein the standard sphere is a lead ball with a diameter of 1 cm.
3. The method for detecting the growth state of nucleated pearls in living pearl oysters
according to Claim 1, wherein in Step (2), the method of identifying the boundary points
of the initial image of the standard sphere is to distinguish the pixels of the initial image
according to the gray value of each pixel: when the gray value of a pixel is higher than a
gray threshold, the pixel is a pixel that constitutes the initial image; when the gray value of
a pixel is lower than the gray threshold, the pixelisapixel that constitutes the background;
and in two adjacent pixels, when a first pixel is a pixel that constitutes the initial image
and a second pixel is a pixel that constitutes the background, taking the first pixel as a
boundary point of the initial image, all the pixels are detected one by one to obtain all the
boundary points of the initial image.
4. The method for detecting the growth of nucleated pearls in living pearl oysters
according to Claim 1, wherein in Step (4), the method of identifying the boundary points
of the nacre image is to distinguish the pixels of the nacre image according to the gray
value of each pixel: when the gray value of a pixel is higher than a gray threshold, the
pixel is a pixel that constitutes the nacre image; when the gray value of a pixel is lower
than the gray threshold, the pixel is a pixel that constitutes the background; and in two
adjacent pixels, when a first pixel is a pixel that constitutes the nacre image and a second
pixel is a pixel that constitutes the background, taking the first pixel as a boundary point of
the nacre image, all the pixels are detected one by one to obtain all the boundary points of
the nacre image; and the method of identifying the boundary points of the nucleus image
is the same as above.
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CN113030130A (en) * 2021-02-24 2021-06-25 中国水产科学研究院渔业机械仪器研究所 Shellfish fullness degree judging method and system

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