AU2017385419A1

AU2017385419A1 - Method for eliminating suspended dust originating from particulate tailings generated by means of wind erosion, comprising obtaining a biological composition, applying the biological composition, and stabilising the particulate matter, as well as the resulting biological composition and the application thereof

Info

Publication number: AU2017385419A1
Application number: AU2017385419A
Authority: AU
Inventors: Daniel BARROS VÁSQUEZ; Alejandro MUÑOZ ROJAS; Claudia Andrea ORTIZ CALDERÓN; Jaime PIZARRO KONCZAK; Marcela Andrea WILKENS ANWANDTER
Original assignee: Universidad de Santiago de Chile
Current assignee: Universidad de Santiago de Chile
Priority date: 2016-12-30
Filing date: 2017-12-28
Publication date: 2019-08-15
Anticipated expiration: 2037-12-28
Also published as: WO2018119541A1; AU2017385419B2; PE20191325A1; BR112019013552A2; CL2016003432A1

Abstract

The present invention relates to a method for eliminating suspended dust originating from particulate tailings generated by means of wind erosion, comprising obtaining a biological composition, applying the biological composition, and stabilising the particulate matter. The invention also relates to the resulting biological composition and to the application thereof to particulate tailings.

Description

SPECIFICATION

FIELD OF APPLICATION

In general terms, this invention describes a method to eliminate airborne dust coming from particulate matter, specially from tailings, and biological compounds, comprising blends of cyanobacteria and microalgae obtained from deposits under the numbers KCTC13158BP and KCTC13159BP.

INVENTION BACKGROUND

In the state of the art it is well known that industrial activities, transportation, and natural events such as wind erosion lead to emissions of particulate matter from the surface of mining deposits. The foregoing becomes an environmental problem, as this particulate matter can carry toxic/dangerous materials, such as mining tailings. These substrata are a suspension of fine solids in a liquid, mainly made up of the same material existing in the orebody, from which a portion of material with valuable ore has been extracted. These tailings are mainly made up of particles smaller or equal to 10 microns, with a high/varied content of minerals. As they are spread by the wind, these particles impact on the environment, thus causing high environmental impact.

In the state of the art we may find assorted strategies to control particulate matter coming from various sources, which, in one way or another, use biological components. However, these require multiple applications, with no final solutions achieved, thus increasing maintenance costs.

For instance, document US 2013/0196419 describes a composition aimed to reduce airborne/liquid-contained particulate matter which comprises a source of Exopolysaccharides selected from silt-producing microorganisms strains, a microorganism with ureolytic activity and a culture means, where the silt-producing microorganisms are selected from microalgae and the microorganism with ureolytic activity is a culture of Bacillus pasteurii. Claim CL 0241-2012 from the same Petitioner, describes a method to reduce airborne/water-contained particulate matter comprising agglomeration of airborne/water-contained particulate matter with negatively charged Exopolysaccharides (EPS), where the microorganism producing the negatively charged EPS is a bacteria or a microalgae.

Regarding cyanobacteria, some documents describe their capacity/ relationships to remove metal/organic contamination.

The non Patented document “Exopolysaccharide-producing cyanobacteria in heavy metal removal from water: molecular basis and practical applicability of the biosorption process”, written by R. Philippis, G. Colica, and E. Micheletti, specifically describes that microorganisms as Exopolysaccharides (EPS) on the cell surface of a cyanobacteria are capable to eliminate metals from contaminated environments, by means of various mechanisms, such as metabolic processes or by absorption of metals in ion-charged macro-molecules from the cell surface. In this same line, the document “Characterization and Optimization of Bioflocculant Exopolysaccharide Production by Cyanobacteria Nostoc sp. BTA97 and Anabaena sp. BTA990 in Culture Conditions” written by OR. Tiwari and colleagues, describe proved experiments where production of Exopolysaccharides (EPS) from 40 cyanobacteria strains were used as bioflocculants aimed to absorb or eliminate organic contaminants, and as an alternative to various environmental bio-technological applications. Just like the previous document, the scientific article named “Production of exopolysaccharides by the cyanobacterium Anabaena sp. BTA992 and application as bioflocculants’’, written by R. Khangembam, OR. Tiwari and M. Kalita, describes the effect of Exopolysaccharides (EPS) from 10 cyanobacteria strains during their photoautotrophic culture. The results proved that cyanobacteria selected from Anabaena sp., may become a good candidate for commercial production of EPS and may be used in applications, as an alternative to synthetic flocculants.

All those quoted documents use Exopolysaccharides as bio-flocculants and are used for removing heavy metals in contaminated liquids, but none of them has solved the technical problem of eliminating airborne dust caused by wind erosion, coming from particulate matter tailings. In order to solve this problem, this invention provides the application of a new biological compound which includes blends of cyanobacteria and microalgae. This invention has the advantage to stabilize airborne dust coming from tailings particulate matter, which minimizes water infiltration and erosion caused by the wind in mining tailings.

DESCRIPTION OF THE INVENTION

This invention deals with a method aimed to eliminate airborne dust coming from tailings particulate matter, caused by the wind erosion. Such method includes to obtain biological compounds from a proper liquid culture means; to apply a certain volume of biological compound to the tailings or substratum to be treated; and to stabilize particulate matter.

Besides, biological compounds are claimed to be made from microorganisms blends obtained from deposits KCTC13158BP and KCTC13159BP, suspended in a proper culture means.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 describes a photo collection on typical performance of the non treated test tubes, during the execution of the tests in a wind tunnel, at various times: 0 minutes (Fig. 1a), 5 minutes (Fig. 1b) and 20 minutes (Fig. 1c).

Figure 2 describes a photo collection on typical performance of the test tubes with Ph1 treatment, during the execution of the tests in a wind tunnel, at various times: 0 minutes (Fig. 2a), 10 minutes (Fig. 2b), 20 minutes (Fig. 2c), 60 minutes (Fig. 2d), 120 minutes (Fig. 2e) and 180 minutes (Fig. 2f).

Figure 3 describes a photo collection about the typical performance of the test tubes treated with the blend 1 of cyanobacteria, during the execution of the wind tunnel tests, at various times: 0 minutes (Fig. 3a), 10 minutes (Fig. 3b), 20 minutes (Fig. 3c), 60 minutes (Fig. 3d), 180 minutes (Fig. 3e) and 360 minutes (Fig. 3f).

Figure 4 describes the weights (Fig. 4A) and tailings loss percentage (Fig. 4B) because of wind effects, recorded in the wind tunnel tests, for non treated tailings samples. The black bars describe the initial dry weight; the white bars describe the final dry weight.

Figure 5 describes the weights (Fig. 5A) and tailings loss percentage (Fig. 5B) because of wind effects, recorded during the wind tunnel tests for tailings samples with Ph1 treatment. The black bars describe initial dry weight; the white bars describe the final dry weight.

Figure 6 describes the weights (Fig. 6A) and tailings loss percentage (Fig. 6B) because of wind effects, recorded in the wind tunnel tests for tailings samples treated with the blend 1 of cyanobacteria. The black bars describe the initial dry weight; the white bars describe the final dry weight.

Figure 7 describes the weights (Fig. 6A) and tailings loss percentage (Fig. 6B) because of wind effects, recorded in the wind tunnel tests for tailings samples treated with the blend 2 of cyanobacteria and microalgae. Black bars indicate the initial dry weight; the white bars indicate the final dry weight.

DETAILED DESCRIPTION OF THE INVENTION

This invention describes a method and a composition aimed to eliminate airborne dust coming from tailings particulate matter, where such method comprises the following stages:

a. Getting biological compounds in a proper liquid culture means, made up of a blend of microorganisms, which is obtained from deposits KCTC13158BP and KCTC13159BP;

b. To apply sprinkler irrigation once, between 100 to 200 (cm³) of the biological compound every 500 to 1.000 (cm²) of tailings surface or substratum to be treated, with an irrigation rate between 2 and 2,5 (L/m²);

c. To stabilize particulate matter in such a way to avoid wind erosion or spread of such particulate matter from the surface of the substratum.

Biological compounds were obtained by growing soil samples crusts in proper sterile liquid means. The whole protocol comprises the following:

• Tubes incubation, performed by applying 12-hour light/12-hour darkness photoperiods on the samples, at 28°C;

• Sowing and cultivation in proper solid sterile medium • Incubation by applying 12-hour light/12-hour darkness photoperiods, at 28°C thus obtaining isolated colonies;

• Sequencing the isolated colonies obtained;

• Generation of liquid cultures from the featured isolated colonies;

• Cryopreservation, by centrifuging 2 mL of culture from each isolated colony, featured with cyanobacteria and microalgae at 4.000 RPMs, during 10 minutes;

• Discarding the supernatant and resuming suspension of the precipitate in proper liquid means;

• Cooling the suspension during 5 minutes, at 3°C; later, during 30 minutes at -20°C and so on, freezing at -86°C;

• Unfreezing the cryo-preserved samples, performing an inoculation at 10% v/v from the mother culture in proper nutrient medium, incubating during 14 days, at 3.000 lux, with a 14/12-hour light cycle and orbital stirring, at a constant speed of 120 RPMs.

The proper media for cultures are selected among the following:

• Culture of soil samples crusts: BG11 or MDM;

• Solid sterile medium: BG11;

• Media to cryopreserve the samples: medium BG11 with dimethylsulfoxide at 3% v/v and methanol at 5% v/v or in medium BG11 with glycerol at 10% v/v;

• Medium to unfreeze the phial glasses: nutrient medium containing N:P:K and trace elements Ca, Fe, Mg, Mn and S.

Besides, in this invention biological compounds are claimed, including:

• Microorganisms blends obtained from deposits KCTC13158BP and

KCTC13159BP with a concentration between 10⁶ to 10⁸ cells per mL; and • Culture means.

Microorganisms of the biological compound may be selected, but are not limited to the genders of the cyanobacteria Leptolyngbya and Trichocoleus. These may be selected, but are not limited to the species Leptolyngbya badia, Trichocoleus sociatus, Trichocoleus desertorum Leptolyngbya boryana, Leptolyngbya sp. The microalgae may be selected, but are not limited to species of the gender Chlorella. Besides, it is possible to use combinations of such cyanobacteria and microalgae.

It is well known that cyanobacteria and microalgae are capable to grow on soil whose grain size has a range between 1 to 125 pm, by forming biocrusts facilitating recovery of depleted land, as they favor the growth of plants and vegetables on fine grain size soil. However, the method and the biological compound described in this invention are aimed to stabilize airborne dust coming from the tailings particulate matter, which minimizes water infiltration and wind erosion in mining tailings. There is no background saying that cyanobacteria and microalgae are used with the purpose described in this invention.

EXAMPLES

Example 1: obtainment of the cultures used during stabilization tests

1. Initial samples were obtained from soil located in the Fourth Region of Coquimbo, located in a semi arid zone, located at the west area of South America, in the southern corner of the Big Atacama Desert (29°00’S; 32°10’S). In 6 different areas from the Fourth Region of Coquimbo, 34 soil samples were obtained. The sampling areas were chosen, based on apparent color and texture. For obtaining the samples, the first layer of soil was carefully removed (about 1 cm deep) collecting between 8 to 15 grams of soil to be stored in sterile Falcon tubes. Once all samples were collected they were kept at 4°C.

2. At the same time, in order to isolate the microorganisms, about 1 gram was taken from each sample of soil and 10 mL of sterile culture means were added (BG11 or MDM) using sterile Falcon tubes of 15 mL. With smaller quantities the proportion of 1/10, was always respected; i.e., per each gram of soil 10 mL of medium was added. The blend was later agitated in an orbital shaker, during 30 minutes, at 34°C. Particulate matter was settled and a 100-pL aliquote was obtained. This was rake sowed in a sterile environment in a BG11 solid medium, covering all the plate. Each plate was tagged with the sample information, inoculated volume and date. The tubes containing liquid means with a proportion of 1/10 and the sowed plates were incubated in a culture chamber, with a 12-hour light/12-hour darkness photoperiod, at 28°C. The liquid cultures were left in the culture chamber until some dark green filaments or clear green stains appeared. An optical microscopy analysis was performed, with a Zeiss Axiostarplus® microscope, at 100X with 10X, in order to confirm presence of microorganisms. A portion of the BG11 liquid sterile medium was transferred. In case of the solid medium plates with evidence of growing pigmented colonies, an optical microscopy analysis was performed and they were sowed in solid BG11 plates solid in order to separate the colonies. They were also inoculated in liquid means in order to obtain biomass.

3. The samples were identified by obtaining genomic DNA for which 200 pl_ of the culture were taken, taking care to include filaments when collecting the material. In case of more compact microorganisms, these were removed from the culture means and a portion was obtained by carefully removing it with to sterile scalpel. For solid medium cultures, some sections were cut with a sterile scalpel. All samples were introduced in the collection tubes and if necessary, they were filled evening the top up to 200 μΙ_ with sterile water. For extracting the Genomic DNA the FavorPrep kit, Soil DNA isolation minikit of Favorgen Biotech corp® were used. DNA was counted and an amplification of DNA fragments was made, aimed to determine presence of microorganisms in the samples. 25 μΙ_ of PCR products from the amplification of each sample were submitted to Macrogen Company for its sequencing. Once the results of the sequencing were known each sequence was processed with the software Geneious in order to detect presence of primers and assess quality of the sequencing provided by electropherograms of the sequencing performed. After that, each sequence was aligned with the tool BLASTn for each gender of microorganism. After that, the list of significant results and the results of the distance tree, delivered by the same tool were analyzed, thus identifying the most likely gender those microorganisms could belong to. These analysis stated that microorganisms present in the samples extracted from soil crusts were cyanobacteria, specifically belonging to the species Leptolyngbya badia, Trichocoleus sociatus, Trichocoleus desertorum Leptolyngbya boryana, Leptolyngbya sp. Besides, the presence of the gender of microalgae Chlorella was identified.

4. In order to obtain the cultures used in the stability analysis, an inoculation at 10% v/v of the cryopreserved culture of the species of cyanobacteria and isolated microalgae was performed. The samples were grown during two weeks in a nutrient medium containing a proportion of N:P:K and trace elements: Ca, Fe, Mg, Mn and S. Growth light conditions had 3,000 lux coming from fluorescent light (40,5 pmol m-2 s1), orbital stirring at constant 120 RPMs, 14/12-hour light cycle, humidity between 40% and 60%.

Example 2: Cryopreservation of the samples ml of culture of each isolated sample was centrifuged at 4,000 RPMs, at ambient temperature during 10 minutes. Next, the supernatant was removed and the precipitate was re-suspended in a cryopreservation medium. Two cryopreservation media were used for preservation at -86°C:

a) Medium AT: BG11 (ATCC Medium 616), dimethylsulfoxide (DMSO in a concentration at 3% v/v and methanol with a concentration at 5% v/v).

b) Medium B: BG11 and glycerol in a concentration at 10% v/v.

Once the cyanobacteria and microalgae biomass was resuspended, the new suspension was cooled during 5 minutes, at 4°C; 30 minutes, at -20°C; and the culture was finally frozen at -86°C.

The samples obtained from soil crusts have been stored under the specifications provided by the Budapest Treaty in the International Authority of Deposits “Korean Collection for Type Cultures’’, under the numbers KCTC 13158BP and KCTC 13159BP. Such stored samples contain the cyanobacterial species Leptolyngbya badia, Trichocoleus sociatus, Trichocoleus desertorum Leptolyngbya boryana, Leptolyngbya sp. and microalgae of the gender Chlorella.

Example 3: Cell Feasibility Assessment of the Cryopreserved Samples

Prior to the feasibility assessment the cultures kept at -86°C were unfrozen, keeping each sample at 4°C. After this they were centrifuged at 4,000 RPMs, during 5 minutes at 4°C. After this the supernatant was removed and all the precipitated biomass was inoculated in liquid/solid means (BG11), at a concentration of 10% v/v.

In order to assess the feasibility of the isolated cryopreserved samples on solid nutrient culture means (BG11 with 1% agar) were inoculated and were kept during 7 days at 3,000 lux coming from fluorescent light (40,5 pmol nr² s^-1), 14/12hour light cycle, humidity between 40% and 60%. In this case feasibility was checked by watching growth in the plate.

In order to assess feasibility in liquid means, the biomass of cryopreserved cyanobacteria and microalgae on nutrient culture means (BG11) was inoculated and the culture was kept, during 30 days, under the following conditions of growth: 3,000 lux coming from fluorescent light (40,5 pmol nr² s^_1), 14/12-hour light cycle, humidity between 40% and 60%, and orbital stirring at a constant speed of 120 RPMs. In this case the feasibility is checked by growth, by observation of the grown biomass, after one and two weeks.

Example 4: Geotechnical Featuring of the tailings material

Lab tests were performed in order to assess the geotechnical composition of the tailings material by measuring the grain size parameters, using a mechanical method (Table # 1) and grain size, using hydrometer method, as per ASTM Standard (Table # 2).

Table 1: Grain size by mechanical method

Screen	#4	#10	#40	#100	#200
Passing %	100	100	91	39	14

Table 2: Grain size by method of the hydrometer (ASTM Standard)

Diameter (mm)	0.06407	0.04686	0.03445	0.02499	0.01325	0.00953	0.00677	0.00340	0.00242
Passing %	8.3	6.9	5.1	3.9	2.7	2.1	1.8	1.3	0.98

Besides, the specific gravity parameters were measured (Table # 3), maximum compacted density (Table # 4) and loose apparent density (Table # 5) for the tailings samples.

Table 3: Specific gravity

Sample #	Specific gravity (kgm/Ns²)
1	2.72

Table 4: Maximum compacted density

Sample #	Max, Humid Density (t/m³)	Max, Dry Density (t/m³)	Optimum Humidity (%)
1	1.9	1.7	15.5

Table 5: Loose Apparent Density

Sample #	Loose Density (t/m³)
1	1.3

Example 5: Wind Tunnel Tests

Wind tunnel tests were performed according to the Chilean Standard NCh 3266 - 2012 on tailings deposits, simulating speed conditions and wind conditions existing at mining sites impacted by wind erosion, so that results are extrapolable to the field conditions.

a) Preparation of the test tubes: Preparation of the test tubes included filling metal molds with tailings with loose apparent density. In order to do so a funnel was used which allows free/constant fall of the tailings until filling its capacity, and finally evening the surface. The average volume of the test tubes is 3,804 cm³. The test tube was prepared and left alone for about 12 hours, before it was subject to wind tunnel tests. The samples treated with the mitigation agent (Ph1) and with the microorganisms were subject to surface irrigation. For a surface of 726 cm² volumes and irrigation rates used are described in Table # 6. Based on the cryopreserved samples two microorganisms blends were prepared: Blend # 1 contains Leptolyngbya badia, Trichocoleus sociatus. Blend # 2 contains Trichocoleus desertorum, Leptolyngbya boryana and Chlorella.sp.

Table 6: Irrigation Rate and Volume applied on Samples

Sample #

Treatment

Watering Rate

Applied Volume

		(L/m²)	(cm³)
1	Ph1	2.06	150
2	Blend # 1	2.06	150
3	Blend #2	2.06	150

b) Installation of the test tube and test execution: The test tube was set on a configurable stand at the wind tunnel. The inclination for this series of tests was between 9° and 10° regarding the horizontal plane. Once the test tube was placed on the stand the test was started by applying wind at a speed of 12 m/s, with time exposure of six hours in a row. In every test initial weight/humidity for each test tube was taken. After finishing the test the leftover material was weighed in the mold and final humidity was measured. Each of the tests performed was photographed. In case of non-treated test tubes a sample was taken every 5 minutes. In case of treated test tubes, samples were taken every 5 minutes until reaching 20 minutes. After 60 exposure and later, after one hour till completing six-hour tests. Figures #1 to #3 show pictures on typical performance of the non treated test tubes (Figure #1), treatment with the mitigation agent Ph1 (Figure 2), and treatment with the blend # 1 (Figure 3), at different times during the execution of the tests subject to wind conditions in the wind tunnel. For the test with blend # 2, there are no pictures.

c) Results from Wind Tunnel Tests: Tests were performed on twelve test tubes, according to the Tailings Deposits Chilean Standard NCh 3266 - 2012, from which three of them were not treated; three of them were Ph1 treated;

three of them were treated with blend #1; and three others were treated with blend #2. Weights and tailings loss percentage, as per wind conditions recorded in the tests performed, are described in Table #7 and in Figures #4 (No treatment), #5 (Ph1 Treated), #6 (Treatment with the blend #1) and #7 (Treatment with blend #2). It is important to highlight that in case of nontreated test tubes, loss of material was reached after 20 minutes, just as described in Figures #1 and #4. In case of the Ph1-treated test tubes, loss of material was recorded after three and six hours of exposure of the test tube into the wind tunnel, as described in Figures # 2 and #5. For test tubes treated with blend #1, there was no loss of particulate matter, just as described in Figures #3 and #6. This result was also observed when treating particulate matter coming from tailings with the blend # 2, just as described in Figure #7.

Table 7: Results of Weights and Tailings Loss Percentage

Test tube #	Test	Dry Density (t/m³)	% DSMC P.N.	Wind Tunnel Tests	Tailings Loss caused by Wind Conditions
Initial Dry Weight	Final Dry Weight	Final Dry Weight
(gr)	(%)	(gr)	(%)	(gr)	(%)
1	s.t.	1.37	81	5125	100	230	4.5	4895	95.5
2	s.t.	1.34	80	5167	100	392	7.6	4775	92.4
3	s.t.	1.34	80	5110	100	344	6.7	4766	93.3
1	Ph1	1.31	78	4976	100	1	0.0	4975	100.0
2	Ph1	1.31	78	5138	100	1	0.0	5137	100.0
3	Ph1	1.32	78	5118	100	6	0.1	5112	99.9
1	Blend # 1	1.34	80	5130	100	5093	99.3	37	0.7
2	Blend # 1	1.31	78	5107	100	5077	99.4	30	0.6

3	Blend # 1	1.31	78	5086	100	5056	99.4	30	0.6
1	Blend #2	1.32	79	5054	100	4893	96.8	161	3.2
2	Blend #2	1.32	78	4899	100	4743	96.8	156	3.2
3	Blend #2	1.32	78	4976	100	4823	96,9	153	3,1

s.t.: No Treatment Tests

As this invention has been described under the foregoing modalities, it may seem evident that other alternatives, modifications or changes could provide the same results; however, we have figured out that proportions of the cyanobacteria and algae are fundamental for the success of the methodology. Therefore, the modalities of this invention must be considered as an illustration, but not as a limitation. Several changes may be made without losing the spirit and scope of this invention, just as defined in the following claims.

All Patents, Patents requests, Scientific Articles and other public documents that, according to the Petitioner are deemed as state of the art, have been properly quoted in this request.

Claims

1. A method to eliminate airborne dust coming from tailings particulate matter, wherein said method comprises the following steps:

a. Getting biological compounds in proper liquid culture means, made up of microorganisms blends obtained from the deposits KCTC13158BP and KCTC13159BP;

b. Apply once, between 100 to 200 cm³ of a biological compound every 500 to 1,000 cm² of surface of tailings or substratum to be treated, with an irrigation rate between 2 and 2.5 L/m²;

c. Stabilize particulate matter.

2. The method of the Claim 1, wherein the application of the biological compound is made by sprinkler irrigation.

3. The method of the Claim 1, wherein the biological compounds are obtained when growing soil samples crusts in proper sterile liquid means; tubes incubation with samples, applying 12-hour light/12-hour darkness photoperiods at a temperature of 28°C; growing in proper solid sterile medium and incubation applying 12-hour light/12-hour darkness photoperiods at a temperature of 28°C in order to obtain isolated colonies; sequencing of the isolated colonies obtained; cryopreservation by centrifuging 2 mL of culture for each isolated sample and centrifuged at 4,000 RPMs during 10 minutes; removing the supernatant and re-suspend the precipitate in proper liquid means; cooling the suspension during 5 minutes at 3°C, later 3 during 30 minutes at -20°C and freezing at -86°C; unfreeze the cryopreserved samples by inoculating at 10% v/v from the mother culture in proper nutrient medium, incubating during 14 days at 3,000 lux with 14/12-hour light cycle and orbital stirring with constant speed at 120 RPMs; mixing the species of cyanobacteria and/or microalgae; growing till getting the necessary volumes and cell density of 10⁶ to 10⁸cells per mL.

4. The method of the claims 1 to 3, wherein the proper sterile liquid means used to grow the soil samples crusts may be selected between medium BG11 or MDM.

5. The method of the Claims 1 to 3, wherein the proper solid sterile medium is solid BG11 medium.

6. The method of the Claims 1 to 3, wherein the proper medium used for cryopreservation is selected between medium BG11 with dimethylsulfoxide at 3% v/v and methanol at 5% v/v or in medium BG11 with glycerol at 10% v/v.

7. The method of the Claims 1 to 3, wherein the proper medium for unfreezing the phial glasses of the samples is a nutrient medium containing N:P:K and trace elements of Ca, Fe, Mg, Mn and S.

8. A biological compound acting as dust suppressor coming from tailings particulate matter, wherein comprises:

• A blend of microorganisms obtained from the deposits KCTC13158BP and KCTC13159BP, in a concentration between 10⁶ to 10⁸ cells per mL; and • Culture means.

9. The biological compound of Claim 8, wherein the microorganisms are selected from cyanobacteria, more specifically are selected between the species

Leptolyngbya badia, Trichocoleus sociatus, Trichocoleus desertorum Leptolyngbya boryana, Leptolyngbya sp., or combinations thereof.

10. The biological compound of Claim 8, wherein the microorganisms are selected from microalgae ofthe gender Chlorella sp.

11. The biological compound of the Claim 8, wherein the culture means is selected between liquid sterile means BG11, MDM, or nutrient medium containing N:P:K and trace elements of Ca, Fe, Mg, Mn and S.

12. The use of the biological compound, wherein it is used to eliminate airborne dust coming from mining tailings particulate matter, produced in general in industrial activities, transportation businesses, and natural events, such as wind erosion.