AU2017100096A4 - A simulated experimental device of vertical migration of algae and its application - Google Patents

A simulated experimental device of vertical migration of algae and its application Download PDF

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AU2017100096A4
AU2017100096A4 AU2017100096A AU2017100096A AU2017100096A4 AU 2017100096 A4 AU2017100096 A4 AU 2017100096A4 AU 2017100096 A AU2017100096 A AU 2017100096A AU 2017100096 A AU2017100096 A AU 2017100096A AU 2017100096 A4 AU2017100096 A4 AU 2017100096A4
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algae
container
control system
migration
illumination control
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AU2017100096A
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Lijuan Cui
Di HUANG
Yinru Lei
Chunyi Li
Wei Li
Xu PAN
Weigang XU
Manyin Zhang
Xinsheng Zhao
Li Zhu
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Research Institute of Forestry New Technology of Chinese Academy of Forestry
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Research Institute of Forestry New Technology of Chinese Academy of Forestry
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Abstract

\\davies.Iocal'meldfs\redirected\sxd\Desktop\Nitro Pro 9 Worldng Folder\innov spei - 35262120.docx-23/01/2017 Abstract The invention discloses a simulated experimental device of vertical migration of algae and its application. The device provided by this invention includes the illumination control system (1), the wind power regulating system(2), the temperature control system and the cylinder container (3) which has an opening at its top and used as the container of migration of algae. The illumination control system (1) mentioned above is set at the top of the container (3) and fixed at the outer surface of the container by ways of connection. The wind power regulating system(2) is set at the opening on the top of the container(3) and can be fixed on the bottom of illumination control system(1). The temperature control system can be set at the inner surface of the container (3) by ways of bonding. Indoors simulated experiment of vertical migration of algae can be conducted by using the simulated experimental device of vertical migration of algae. The device can be used to simulate the temperature, illumination and hydrodynamic effect on the algae vertical migration. \\daoies.Iocaloieldfs\edircred\xd\lDeskrop\Niro Pro 9 Worn 9 E~oldor\inoo spori - 35262120.do-23/OI/2017 2 1 1 t± ts. IallI Fi 1

Description

\\davies.local\meldfs\redirected\sxd\Desktop\Nitro Pro 9 Working Folder\innov sped - 35262120.docx-23/01/2017 2017100096 25 Jan 2017 - 1 - A Simulated Experimental Device of Vertical Migration of Algae and Its
Application
Technical field [0001] The invention relates to the field of ecological engineering technology, especially involves a simulated experimental device of vertical migration of algae and its application.
Background technology [0002] In the late spring and early summer, the physical and chemical properties of the water-sediment interface and the environmental conditions of the lake changed significantly with the increase of temperature, the enhancement of light and wind speed and other climatic conditions. A large number of algae cells began to recover from the bottom of the lake and in the favorable conditions of light, temperature and hydrodynamic, floating to the water.
[0003] The migration of algae is restricted by the site, and the current research has not been used for simulating the algae horizontal-vertical migration, especially the simulated experimental device of vertical migration of algae.
Summary of the invention [0004] In order to compensate for the deficiencies in the above fields, the present invention provides a simulated experimental device of vertical migration of algae.
[0005] The simulated experimental device of vertical migration of algae provided in this invention includes the illumination control system(l), the wind power regulating system(2), the temperature control system and the cylinder container 0) which has an opening at its top and used as the container of migration of algae. The illumination control system (1) mentioned above is set at the top of the container(3) and fixed at the outer surface of the container by \\da vies.local\meldfs\redirected\sxd\Desktop\Nitro Pro 9 Working Folder\innov sped - 35262120.docx.-23/01 /2017 2017100096 25 Jan 2017 -2- ways of connection. The illumination control system is set at the place 8-12cm above the container made of polymethyl methacrylate for light compensation of algae, which is flexibly dismantled so that the experimental water can be trickled into the cylinder container made of polymethyl methacrylate.
[0006] The wind power regulating system (2) is set at the opening on the top of the container (3) and can be fixed on the bottom of illumination control system (1). It can move up and down to simulate the effect of natural wind on the algae vertical migration.
[0007] The temperature control system can be set at the inner surface of the container (3) by ways of bonding. It can be flexibly dismantled.
[0008] There are closable holes on the side wall of the container (3) of algae migration, which are used for sampling from the container (3) of algae migration .
[0009] The illumination control system (1) consists of incandescent bulbs and conducting wire (4). The light is supplied by the conducting wire (4), and/or the wind power regulating system(2) is a fan. The fan (2) is fixed on the bottom of the illumination control system (1) by screws with nuts at both two ends, and/or the temperature control system consists of heating rods (11) and switch (10). The heat is produced by electricity transmitted by the conducting wire. The temperature regulation of different water layers in the cylinder container which made of polymethyl methacrylate was realized by switching the heating rod switch, which was used to study the effect of water temperature on the vertical migration of algae.
[0010] The material of the container (3) of migration of algae is polymethyl methacrylate.
[0011] The illumination control system (1) is fixed on the outer surface of the container of algae migration through a bracket (12) with two nuts to replenish illumination for algae. \\davies.local\meldfs\redirected\sxd\Desktop\Nitro Pro 9 Working Folder\innov sped - 35262120.docx-23/01/2017 2017100096 25 Jan 2017 -3- [0012] The fan is at the opening on the top of the container (3). It can move up and down to simulate the effect of natural wind on the algae vertical migration.
[0013] A temperature control system carrier (7) may be set on the inner wall of algae migration container (3) which is used for placement of temperature control system.
[0014] It also consists of tubular sampling components(6) which are composed of a tubular body with both ends open and a sealing cap.
[0015] The tubular body may be embedded in a side wall of algae migration container (3) through the hole and extend into algae migration container (3) to take samples. The tubular body is matched with the hole to achieve a sealed connection. The sealing cap closes the hole by closing the tubular body.
[0016] The container of migration of algae (3) has a plurality of holes which are equidistantly spaced from the top to the bottom and inlaid the corresponding number of tubular sampling components (6).
[0017] The tubular body is made of polymethyl methacrylate.
[0018] The cylinder container which is made of polymethyl methacrylate has a height of 1.0-2.0m and a diameter of 1.0-1.5m; 11 sample tubes are arranged on the cylinder container made of polymethyl methacrylate, the sampling tube is composed of tubes which is made of polymethyl methacrylate and a sealing cap. The distance between the sampling tube (3) at the top and the top edge of the cylinder container is 15 to 20 cm. The sampling tube at the bottom is close to the bottom surface of the cylinder container. The average distance between the sampling tubes are 10 to 25cm.
[0019] The cylinder container (3) made of polymethyl methacrylate is located on the simulator load-bearing platform (8), the simulator loading platform (8) is supported by the simulator bearing strut(9). \\davies.local\meldfs\redirected\sxd\Desktop\NitroPro 9 Working Folder\iimov sped - 35262120.docx-23/0l /2017 2017100096 25 Jan 2017 -4- [0020] The application of the simulated experimental device of vertical migration of algae in indoor simulation of natural vertical migration of algae is also within the scope of the present invention.
[0021] The simulated experimental device of vertical migration of algae provided by the invention can be applied in indoor simulation experiment of natural vertical migration of algae. It can simulate the effects of temperature, light and hydrodynamics on the vertical migration of algae.
Brief description of the drawings [0022] Fig 1 is a simulated indoor experimental device of vertical migration of algae.
[0023] Fig 2 is the component of adjustable temperature system.
[0024] Fig 3 is the algae density under different illumination conditions [0025] Fig 4 is the algae density under different temperature conditions [0026] 1-Illumination control system, 2-Wind power regulating system,3-Algae migration container, 4-Conducting wire, 5-Adjustable temperature control system, 6-Tubular sampling components, 7-Adjustable temperature control system carrier, 8-Simulator load-bearing platform, 9-Simulator bearing strut, 10-Heating rods switch, 11-Heating rods, 12-A bracket with two nuts.
Detailed description of the invention [0027] Example 1: A simulated indoor experimental device of vertical migration of algae [0028] As shown in Fig 1 and Fig 2, the simulated indoor experimental device of vertical migration of algae provided by this invention includes the illumination control system 1, the \\da vies.local\nieldfs\redirected\sxd\Desktop\Nitrc> Pro 9 Working Foldertinnov sped - 35262120.docx.-23/01/2017 2017100096 25 Jan 2017 -5- wind power regulating system 2, the temperature control system and the cylinder container 3 which has an opening at its top and used as the container of migration of algae. The illumination control system 1 mentioned above is set at the top of the container 3 and fixed at the outer surface of the container by ways of connection. The wind power regulating system 2is set at the opening on the top of the container 3 and can be fixed on the bottom of illumination control system 1 .The temperature control system can be set at the inner surface of the container 3 by ways of bonding. There are closable holes on the side wall of the container of algae migration 3, which are used for sampling.
[0029] The illumination control system 1 consists of incandescent bulbs and conducting wire 4). The light is supplied by the conducting wire 4. The wind power regulating system 2 is a fan. The fan is fixed on the bottom of the illumination control system 1 by screws with nuts at both two ends. The fan is located between the illumination system 1 and algae migration container 3. The temperature control system consists of heating rods 11 and switch 10. The heat is produced by electricity transmitted by the conducting wire 4. The materials of the container 3 of migration of algae is polymethyl methacrylate. The illumination control system 1 is fixed on the outer surface of the container of algae migration through a bracket 12 with two nuts to replenish illumination for algae. The fan is at the opening on the top of the container 3. It can move up and down to simulate the effect of natural wind on the algae vertical migration.
[0030] In a further embodiment, a temperature control system carrier 7 can be provided on the inner wall of the algae migration container 3 for placing the temperature control system. The adjustable temperature control system carrier 7 is embedded in the inner wall of the organic glass cylinder container 3 by adhesive way, and is arranged in a vertical stripe distribution, and can be flexibly disassembled, and an adjustable temperature control system 5 is arranged on it. The adjustable temperature control system 5 is composed of a heating rod switch 10 and a heating rod 11 which generates heat by supplying electric energy through a \\davies.local\meldfsVedirectedVsxd\Desktop\Nitro Pro 9 Working Folder\innov sped - 35262120.docx.-23/01 /2017 2017100096 25 Jan 2017 -6- conductive wire 4. The algae migration container 3 is located on the simulator loading platform 8 and the simulator loading platform 8 is supported by a simulator supporting column 9.
[0031] In a further embodiment, a tubular sampling assembly 6 is also provided, comprising a tubular body and a closure cap open at both ends. The tubular body can be embedded in a side wall of algae migration container 3 through the hole and extend into algae migration container 3 to take samples. The tubular body is matched with the hole to achieve a sealed connection. The sealing cap closes the hole by closing the tubular body.
[0032] The algae migration container 3 has a plurality of the through holes on the side walls of the algae migration container 3 which are equidistantly spaced from the top to the bottom and are fitted with a corresponding number of the tubular sampling units 6 therein. The tubular body is made of polymethyl methacrylate. The tubular sampling assembly 6 is embedded on the algae transport container 3 at equal distances from the top to the bottom.
[0033] In a further embodiment, the algae migration container 3 has a height of 1.0 to 2.0 m and a diameter of 1.0 to 1.5 m. A total of 11 sampling tubes 6 are provided in the algal migration container 3. The sampling tube 6 is composed of a tube and a sealing cap, wherein the uppermost sampling tube 6 is 15.0 to 20.0 cm away from the top of the cylinder container 3 which is made of polymethyl methacrylate and the lowermost sampling tube 6 is in close contact with the lower edge of the algae transport container 3, and the average distance between adjacent sampling tubes is 10.0-25.0 cm. The lighting control system 1 is composed of incandescent bulbs and a conductive wire 4, and the height of the cylinder container which made of polymethyl methacrylate is 8.0 to 12.0 cm for compensating the light for the algae, which can be flexibly disassembled to enable the algae migration container 3 injecting the test water. The fan is at the opening on the top of the container 3. It can move up and down to simulate the effect of natural wind on the algae vertical migration. The temperature control \\davies.local\nieldfs\redirected\sx.d\Desktop\Nitrc> Pro 9 Working Foldertiimov sped - 35262120.docx.-23/01/2017 2017100096 25 Jan 2017 -7- system 5 consists of adjustable temperature control system carrier 7, heating rods 11 and switch 10. The heat is produced by electricity transmitted by the conducting wire. The temperature regulation of different water layers in the cylinder container 3 which made of polymethyl methacrylate was realized by switching the heating rod switch 10, which was used to study the effect of water temperature on the vertical migration of algae.
[0034] Example 2: Indoor simulated experiment of the migration of algae conducted by using the device provided by this invention [0035] The simulated experiment was divided into two groups, each set 25 algae vertical migration indoor simulation experimental devices (the device design is exactly the same as in Example 1). During the experiment, the light source was adjusted so that the light-dark ratio was 12:12, In the range of 0-6300 lx, the algae culture medium was prepared according to the following table 1 and 2. In the process of preparation, the algae culture medium was first quantitatively arranged according to Table 1, and then the solution (Bactrian culture liquid (B) 1 solution [Euglenose culture solution (A)] was mixed to complete the algal culture preparation. At the beginning of the experiment, the TN, TP and algal densities of the original water samples were measured (TN test refer to HJ636-2012, TP test refer to GB711893-89, algal density test refer to Yu Haiyan, Zhou Bin, Hu Zunying, etc.) 2009(06): 40-43. "), Followed by regular sampling and continuous observation at regular intervals. (2): 11-17.) As the experimental species, the algae blooms were used in the algae blooms, such as Euglena angustifolia (Zhao Xin-sheng, Cui Li-juan, Li Wei, etc.)
Form 1 Culture Solution of Euglena (A)
Item Stock Solution(g/100ml) Culture Solution(ml) Sodium Acetate 10 10 Beef extract 10 10 \\davies.local\meldfs\redirected\sxd\Desktop\Nitro Pro 9 Working Folder\innov sped - 35262120.docx-23/0l /2017 -8-
Tryptose 10 20 Yeast Extract 10 20 soil extraction * / 30 Deionized Distilled Water / 910 2017100096 25 Jan 2017 * Preparation of soil extract: Take a small amount of research area of the shore of the soil, add 2 to 3 multiples the deionized water, boil for 1 hour. It will be used after cooled and filtered through the filter paper.
Form 2 Culture Solution of Euglena ¢)
Item Stock Solution(g/ 100ml) Culture Solution(ml) kno3 1.0 20 (NH4)2HP04 0.2 10 MgS04.7H20 0.1 10 CaS04 Saturated solution 20 (1) Algae vertical migration simulation experiment under different illumination conditions 25 tasks are set up at equal intervals in the light intensity of 0-6300 lx. Meanwhile five different light transmittances (that is, different light intensity) are set up. A light intensity control switch can be installed in the light intensity control device, in the course of the experiment by adjusting light intensity control switch. The light intensity set in the first device is 6300 lx, the light intensity set in the second device is 50401x, the light intensity set in the third device is 40951x, the light intensity set in the fourth device is 25201x, the light intensity set in the fifth device is 15751x. Specific light intensity control is based on the light intensity which light quantum monitors. The light control system which in light algae vertical migration indoor simulation experiment device and the external lighting system around supply the light source. The temperature was maintained at (25 + 1) °C. The light and dark cycle is 12L: 12D. The TN, TP and density of algae at different water levels were sampled at regular intervals. At 2017100096 25 Jan 2017 \\davjes.Iocal\meldfs\redirected\sxd\Desktop\Nitro Pro 9 Working Foldertiimov sped - 35262120.docx -23/01 /2017 -9- the same time, a proper amount of nitrogen or phosphorus nutrients are added to maintain the concentration of nitrogen and phosphorus in original water . The results are shown in Fig 3. (2)Simulated experiment of vertical migration of algae under different temperature conditions The water temperature is set at five grades, 15°C, 20°C, 25°C, 30°C, 35°C.(the grade intervals can be subdivided by practical situation). The water temperature is controlled by heating rods (10). Illumination intensity is controlled by the above way in five different illumination intensities, and the light and dark cycle is 12L: 12D.The TN, TP and different water level algal densities was measured by sampling at regular intervals. At the same time, a proper amount of nitrogen or phosphorus nutrients are added to maintain the concentration of nitrogen and phosphorus in original water. The results are shown in Fig 4.
Results: [0036] The results of algae vertical migration simulation under different temperature showed that the algae were enriched more quickly at 30 °C, and the density of algae was higher than that of the bottom. The simulation results of algae vertical migration under different light show that the concentration of algae to the surface is the highest under 63001x light intensity. The results of the two experiments show that the device is of practical values to simulate the effects of temperature and light on the vertical migration of algae in natural environment.

Claims (10)

  1. Claims
    1. A simulated experimental device of vertical migration of algae is characterized in that it includes the illumination control system(l), the wind power regulating system(2), the temperature control system and the cylinder container (3) which has an opening at its top and used as the container of migration of algae, the illumination control system(l) mentioned above is set at the top of the container(3) and fixed at the outer surface of the container by ways of connection, the wind power regulating system(2) is set at the opening on the top of the container(3) and can be fixed on the bottom of the illumination control system(3), the temperature control system can be set at the inner surface of the container(3) by ways of bonding, there are closable holes on the side wall of the container of algae migration, which are used for sampling.
  2. 2. According to claim 1, the device mentioned above is characterized in that the illumination control system(l) consists of incandescent bulbs and conducting wire(4), the light is supplied by the conducting wire(4), and/or the wind power regulating system(2) is a fan, the fan(2) is fixed on the bottom of the illumination control system(l) by screws with nuts at both two ends, and/or the temperature control system consists of heating rods and switch. The heat is produced by electricity transmitted by the conducting wire.
  3. 3. According to claim 1, the device mentioned is characterized in that: the material of the container (3) of migration of algae is polymethyl methacrylate.
  4. 4. According to claim 1, the device mentioned is characterized in that: the illumination control system (1) is fixed on the outer surface of the container of algae migration through a bracket (12) with two nuts to replenish illumination for algae.
  5. 5. According to claim 2, the device mentioned is characterized in that: the fan is at the opening on the top of the container(3), and it can move up and down to simulate the effect of natural wind on the algae vertical migration.
  6. 6. According to claim 1, the device is characterized in that: a temperature control system carrier (7) can be set on the inner wall of the migration of algae container(3) which is used for placement of temperature control system.
  7. 7. According to claim 1 to claim6, the device is characterized in that: it also consists of tubular sampling components(6) which is composed of a tubular body with both ends open and a sealing cap, the tubular body can be embedded in a side wall of the migration of algae container through the hole and extend into algae migration container to take samples, and the tubular body is matched with the hole to achieve a sealed connection, and the sealing cap closes the hole by closing the tubular body.
  8. 8. According to claim 7, the device is characterized in that: the container of migration of algae(3) has a plurality of holes which are equidistantly spaced from the top to the bottom and inlaid the corresponding number of tubular sampling components(6).
  9. 9. According to claim 7 and claim 8, the device is characterized in that: the tubular body is made of polymethyl methacrylate.
  10. 10. According to claim 1 to claim 9, the application of the device used in indoor simulation of natural vertical migration of algae.
AU2017100096A 2016-07-28 2017-01-25 A simulated experimental device of vertical migration of algae and its application Ceased AU2017100096A4 (en)

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CN201610607919.6 2016-07-28
CN201610607919.6A CN106124708A (en) 2016-07-28 2016-07-28 Algae vertical transfer analogue experiment installation and application thereof

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CN112029652B (en) * 2020-08-13 2024-03-22 宁波大学科学技术学院 Device and method for researching algae phototactic rule
CN116297027B (en) * 2023-05-10 2023-07-25 长江三峡集团实业发展(北京)有限公司 Method and device for measuring migration characteristics of microcystis population

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US8569050B1 (en) * 2009-05-04 2013-10-29 John D. Ericsson Enclosed bioreactor system and methods associated therewith
CN101638620B (en) * 2009-08-28 2012-09-05 南京大学 Method and device for simulating water bloom floatation of blue algae
CN201517107U (en) * 2009-08-31 2010-06-30 环境保护部南京环境科学研究所 Alga experiment analog device for controlling temperature, light and flow rate
CN202583160U (en) * 2012-05-22 2012-12-05 三峡大学 Water bloom alga vertical migration simulation experiment device
CN203187688U (en) * 2013-04-24 2013-09-11 重庆大学 Algae cultivation box
CN205844307U (en) * 2016-07-28 2016-12-28 中国林业科学研究院林业新技术研究所 Algae vertical transfer analogue experiment installation

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