CN110004187B - Method for improving oil production efficiency and carbon sequestration rate of microalgae - Google Patents
Method for improving oil production efficiency and carbon sequestration rate of microalgae Download PDFInfo
- Publication number
- CN110004187B CN110004187B CN201810933261.7A CN201810933261A CN110004187B CN 110004187 B CN110004187 B CN 110004187B CN 201810933261 A CN201810933261 A CN 201810933261A CN 110004187 B CN110004187 B CN 110004187B
- Authority
- CN
- China
- Prior art keywords
- culture
- stage
- microalgae
- culture medium
- production efficiency
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for improving microalgae oil production efficiency and carbon sequestration rate, which comprises the following steps: (1) inoculating microalgae in a culture medium, and performing first-stage culture; (2) after the culture of the first stage is finished, adding a phosphorus source and an iron source into a culture medium, and carrying out the culture of the second stage, wherein the microalgae is chlorella pyrenoidosa, and the culture conditions of the whole culture process are as follows: introducing 15% by volume of CO into the culture medium2As a carbon source, the gas supply rate is 0.1 v/v/m; the temperature is 27 ℃, the pH value is 6.0-8.0, the illumination is 8500Lx, and the light dark period is 16: 8. The method can improve the oil production efficiency and the oil content of the microalgae, and simultaneously greatly improves the carbon fixation efficiency of the microalgae.
Description
Technical Field
The invention belongs to the fields of microalgae biotechnology and carbon emission reduction, and particularly relates to a method for improving microalgae oil production efficiency and carbon fixation rate.
Background
The emission of a large amount of carbon dioxide is one of the main causes of global greenhouse effect, and the emission reduction of the carbon dioxide becomes the key of the emission reduction of greenhouse gas in various countries at present. To control CO2The emphasis on emissions is on controlling the CO produced during the combustion of fossil fuels2Thereby controlling CO generated in the coal combustion process2Is currently carrying out CO2The key to emission reduction lies. In the capture of CO in a plurality of2In the method, the biological resource carbon fixation technology has become international CO2The method is a front-end research hotspot in the fields of emission reduction and new energy development, and has wide application prospect. Meanwhile, the increasing exhaustion and over-development of fossil energy also bring great pressure to the utilization of energy resources, so that the development of renewable eco-friendly fuels is of great significance to the maintenance of good ecological environment and the alleviation of energy crisis. Microalgae is a unicellular or simple multicellular microorganism widely existing on the earth, and is considered as a new generation of biodiesel raw material even capable of completely replacing traditional petroleum diesel due to the advantages of rapid growth, easy large-scale culture, strong environmental adaptability, no land competition for agriculture and the like.
Although there are many advantages to the production of biodiesel from microalgae, the production cost is high and the production cost is still a huge resistance to the actual realization of industrial production. After the research of the national renewable energy laboratory for 18 years, the key influencing the production cost is finally considered not to be related engineering technology but to be economic investment generated for improving the yield of microalgae biomass and grease.
Normally, microalgae cultured under total nutrient conditions can achieve relatively fast growth rates, but the oil content is not high. Numerous studies have shown that adverse growth environment factors can increase microalgae lipid content, such as nitrogen deficiency, silicon deficiency, phosphorus limitation, heavy metal stress, high light and high temperature stress, hormonal effects, and the like, with nitrogen deficiency being the most significant and considered the most effective method for stimulating lipid content increase. However, researchers have found that nitrogen deficiency stimulates an increase in lipid content, often accompanied by cell growth arrest and a decrease in biomass, which results in an ineffective increase in lipid yield. Thus, high oil content and high oil yield are often difficult to achieve simultaneously.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a method for improving the oil production efficiency and the carbon fixation rate of microalgae. The method can improve the oil production efficiency and the carbon fixation rate of the microalgae.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for improving oil production efficiency and carbon sequestration rate of microalgae comprises the following steps:
(1) inoculating microalgae in a culture medium, and performing first-stage culture;
(2) after the first stage of culture, a phosphorus source and an iron source are added to the medium to perform the second stage of culture.
Preferably, the microalgae is Chlorella pyrenoidosa.
Preferably, the culture in the first stage adopts BG11 culture medium, and BG11 culture medium comprises the following components: NaNO31500mg/L，K2HPO4·3H2O 40mg/L，MgSO4·7H2O 75mg/L，Na2CO3 20mg/L，CaCl227mg/L, 6mg/L citric acid monohydrate, 6mg/L ferric ammonium citrate, Na2EDTA 1mg/L, trace element A51mL of solution, wherein, A5The composition consists of the following components: h3BO3 2.86mg/L，MnCl2·4H2O 1.81mg/L，ZnSO4·7H2O 0.222mg/L，CuSO4·5H2O 0.079mg/L，CoCl2·6H2O 0.050mg/L，Na2MoO4·2H2O 0.39mg/L。
Preferably, the whole culture process is carried out under conditions such that 15% by volume of CO is introduced into the culture medium2As a carbon source, the gas supply rate is 0.1 v/v/m; the temperature is 27 ℃, the pH value is 6.0-8.0, the illumination is 8500Lx, and the light dark period is 16: 8.
Preferably, the concentration of the inoculated microalgae is 400-600 mg/L. The inoculation concentration in the range is beneficial to the propagation of algae, and can reach higher biomass faster, and shorten the time of the first stage. Too high inoculation amount can influence the utilization of light and further influence the growth of the algae in the first stage, too low inoculation concentration can cause the biomass of the microalgae to be low, so that too high or too low inoculation concentration can also indirectly influence carbon fixation and oil production.
Preferably, the phosphorus source is inorganic phosphorus.
Preferably, the phosphorus source is one or a mixture of more of disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium dihydrogen phosphate and potassium dihydrogen phosphate.
Preferably, the phosphorus source is K2HPO4·3H2O, adding into post-culture medium PO at one time4 3-The concentration of-P is 60-90 mg/L.
Preferably, the iron source is FeCl3·6H2O, added in one time and added into the post-culture medium3+The concentration of (A) is 1.2-2.4 mg/L.
Preferably, the culture time in the second stage is 4 to 12 days.
The invention has the beneficial effects that: the method provided by the invention has the advantages that in the first stage of full-nutrient culture, sufficient nitrogen source and phosphorus source are provided to obtain higher biomass, in the second stage, grease is rapidly accumulated in the nitrogen deficiency stage, sufficient phosphorus source and iron source are provided at the moment, so that the cells fully accumulate grease, the oil production efficiency and the oil content of microalgae are further improved, the contradiction between the biomass yield and the oil production efficiency of the microalgae is effectively solved, and the carbon sequestration efficiency of the microalgae is greatly improved.
The two-stage culture method is simple and convenient to operate, avoids two-stage culture after microalgae are inoculated again after centrifugation, and is complicated in steps and increased in investment.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the present invention is further described in detail with reference to the following specific examples, which should be noted that the examples are only specific descriptions of the present invention and should not be construed as limiting the present invention.
The chlorella pyrenoidosa used in the present invention is isolated from chlorella pyrenoidosa pieces or extracted from aquatic organisms in the waters of Jiangsu/nearby. However, the method of the present invention is not limited to the Chlorella pyrenoidosa pieces derived from Chlorella pyrenoidosa tablets exemplified in the present invention or Chlorella pyrenoidosa extracted from aquatic organisms in Jiangsu/nearby waters.
The method of the invention can be applied to other non-processed products60CO-gamma ray mutagenesis and CO2Domesticated chlorella pyrenoidosa, but not limited to chlorella pyrenoidosa.
Example 1
(1) Sterilizing BG11 culture medium at 121 deg.C for 30min, cooling to room temperature, packaging into 600mL round bottom glass bottles, inoculating microalgae, and performing first stage culture;
BG11 medium consisted of the following components: NaNO3 1500mg/L，K2HPO4·3H2O 40mg/L，MgSO4·7H2O 75mg/L，Na2CO3 20mg/L，CaCl227mg/L, 6mg/L citric acid monohydrate, 6mg/L ferric ammonium citrate, Na2EDTA 1mg/L, trace element A51mL of solution, wherein, A5The composition consists of the following components: h3BO3 2.86mg/L，MnCl2·4H2O 1.81mg/L，ZnSO4·7H2O 0.222mg/L，CuSO4·5H2O 0.079mg/L，CoCl2·6H2O 0.050mg/L，Na2MoO4·2H2O 0.39mg/L；
In this example, microalgae species are obtained by60CO-gamma ray mutagenesis and tolerance of a volume fraction of 60% CO2The chlorella pyrenoidosa is subjected to high concentration gradient CO2Domesticating, wherein the concentration of inoculated microalgae is 450 mg/L.
The culture conditions of the whole culture process are as follows: introducing 15% by volume of CO2As a carbon source, the gas supply rate was 0.1 v/v/m; the temperature is 27 ℃, the pH is 6.0 to 8.0, the illumination is 8500Lx, and the light-dark period is 16h to 8 h; wherein the gas supply rate is 0.1v/v/m, which means that 0.1 volume of gas is introduced into each volume of culture medium per minute, and the whole culture process comprises a first stage culture and a second stage culture.
After 9 days of culture, the nitrogen source and the phosphorus source in the culture medium are completely consumed, namely the content of the nitrogen source and the phosphorus source can not be detected by the instrument, and the first-stage culture is finished.
(2) In the second stage of culture, the phosphorus source is added into the first stage culture medium once, and added into the post-culture medium PO4 3-The concentration of P is 70mg/L and the addition form is K2HPO4·3H2O, and adding a certain amount of iron source in the form of FeCl3·6H2O, added in one time and added into the post-culture medium3+The concentration of (2) is 1.2-2.4mg/L, the culture process enters a nitrogen-deficiency, phosphorus-enrichment and iron-enrichment stage, and the culture is continued for 12 days.
Example 2
In this example, the microalgae species were not passed through60CO-gamma-ray mutagenesis but with a high concentration gradient of CO2The concentration of the inoculated microalgae of the domesticated chlorella pyrenoidosa is 450 mg/L.
The other steps of this example are the same as example 1.
Comparative example 1
In the second stage of culture, the phosphorus source is added into the culture medium once and added into PO in the post-culture medium4 3-The concentration of P is 70mg/L and the addition form is K2HPO4·3H2O, but no iron source is added, so that the culture process enters nitrogen deficiencyPhosphorus phase, continue culturing for 12 days.
The other steps of this comparative example were the same as example 1.
Comparative example 2
In the second stage of culture, adding phosphorus source into the culture medium, and adding PO into the post-culture medium4 3-The concentration of P is 5mg/L and the addition form is K2HPO4·3H2O, but not adding an iron source, leading the culture process to enter a nitrogen deficiency and phosphorus limitation stage, and continuing to culture for 12 days.
The other steps of this comparative example were the same as example 1.
Comparative example 3
In the second stage of culture, no phosphorus source or iron source is added into the culture medium, so that the culture process enters a nitrogen and phosphorus deficiency stage and continues to culture for 12 days.
The other steps of this comparative example were the same as example 1.
Data determination
The biomass, oil content, oil production efficiency and carbon fixation rate of the microalgae of examples 1-2 and comparative examples 1-3 were measured at total days of culture of 3, 9, 13, 21, respectively.
The biomass measuring method comprises the following steps: measuring biomass by a dry weight method, filtering a certain volume of algae liquid by using a filter membrane with a constant weight and a pore diameter of 0.45 mu m, placing the filter membrane in a drying oven at 105 ℃ until drying, then placing the filter membrane in a dryer to cool to room temperature, and calculating the weight difference before and after weighing to obtain the size of the biomass.
The method for measuring the oil content and the oil production efficiency comprises the following steps:
the calculated Fatty Acid Methyl Ester (FAMEs) content is taken as the oil content, and the FMAEs yield is taken as the oil production efficiency. Simple and rapid methods for FAMEs analysis were used. First, lipids are methyl esterified to fatty acid methyl esters. 20mg of dry algae powder is added into a glass tube with a cover and a volume of 10mL, 2mL of freshly prepared methyl esterification reagent (acetyl chloride/methanol, volume ratio of acetyl chloride to methanol is 1:9) is added, the cover is added and screwed, and then the mixture is placed in a water bath at 80 ℃ for reaction for 2.5 hours. After the reaction was complete, it was cooled to room temperature and 1mL of 1% aqueous NaCl solution and 2mL of n-hexane containing methyl benzoate (approximately 0.36mg/mL n-hexane) were added, with methyl benzoate as an internal standard. After shaking and centrifuging, layering, recovering the upper layer containing FAMEs to a GC sample tube, drying by anhydrous sodium sulfate, and then determining.
The total amount and composition of FAMEs was determined using a gas chromatograph Agilent 6890 using a flame ionization detector and a DB-FFAP model capillary column (30 m.times.0.25 mm.times.0.25 μm). The gas chromatography was run under the following conditions: the sample volume is 1 mu L; the split ratio is 1: 10; the air flow rate is 450mL/min, the H is 240 mL/min, and the carrier gas (N2) is 45 mL/min; the temperature of a sample inlet is 250 ℃; the temperature of the detector is 300 ℃; the initial furnace temperature was 140 ℃ and held for 2min, followed by a 10 ℃/min ramp to 240 ℃ and a 2min hold at 240 ℃. The FAMEs content was calculated from the peak area of each fatty acid methyl ester in the sample and the peak area of the internal standard. The yield of FAMEs was calculated as follows:
where t0 represents the initial time at the time of inoculation and t1 represents the nth day of culture.
CO2The fixed rate calculation method comprises the following steps: analyzing the content of carbon in the algae by using an element analyzer, and calculating CO according to the following formula2Fixed rate:
where t0 represents the initial time at the time of inoculation and t1 represents the nth day of culture.
The biomass, oil content, oil production efficiency and carbon fixation rate of microalgae under the two-stage culture conditions are shown in table 1. The biomass, oil content, oil production efficiency and carbon fixation rate of microalgae under the second stage culture conditions in examples 1-2 and comparative example 3 are shown in table 2.
TABLE 1 Biomass, oil content, oil production efficiency and carbon fixation rate of microalgae in example 1 and comparative examples 1-3 under two-stage culture conditions
TABLE 2 Biomass, oil content, oil production efficiency and carbon fixation rate of microalgae in examples 1-2 and comparative example 3 under the second stage culture conditions
In Table 1, N-P + represents: the culture medium type is nitrogen-deficient and phosphorus-rich, namely, after the first stage is finished, adding rich phosphorus source but not adding iron source into the culture medium, entering the stage of nitrogen deficiency and phosphorus-rich, corresponding to the culture of the second stage of the comparative example 1; N-Plim denotes: the culture medium type is nitrogen deficiency and phosphorus limitation, namely, after the first stage is finished, a small amount of phosphorus source is added, but no iron source is added, and the stage enters a nitrogen deficiency and phosphorus limitation stage, which corresponds to the second stage culture of the comparative example 2; N-P-represents: the type of the medium was nitrogen and phosphorus deficient, which means that after the first stage was completed, the medium was fed to the stage of nitrogen and phosphorus deficiency without adding a phosphorus source and an iron source, corresponding to the second stage of the culture of comparative example 3. N-P + Fe + represents: the culture medium type is nitrogen-deficient, phosphorus-enriched and iron-enriched, which means that after the first stage is finished, a phosphorus source and an iron source are added into the culture medium, and the culture enters a nitrogen-deficient, phosphorus-enriched and iron-enriched stage, corresponding to the culture of the second stage of the example 1; ND means no detection.
The nitrogen and phosphorus deficiency stage is an extension of the first stage in practice because no phosphorus source or iron source is added in the second stage.
As can be seen from Table 1, in the second stage of 4 days of culture, the nitrogen and phosphorus deficiency stage of comparative example 1 has biomass, oil content, oil production efficiency and CO in comparison with the nitrogen and phosphorus deficiency stage of comparative example 3 when the total number of days of culture is 13 days2The fixed rates are 1.2, 2.3, and 1.9 times the latter, respectively; this indicates that the nitrogen-deficient and phosphorus-rich stage can improve the oil production efficiency and CO of the chlorella pyrenoidosa2The rate is fixed.
Second stage culture for 4 daysThe total days of nutrient supply was 13 days, the nitrogen and phosphorus deficient iron rich phase of example 1 compared to the nitrogen and phosphorus deficient phase of comparative example 3, the biomass, oil content, oil production efficiency and CO of example 12The fixed rates were biomass, oil content, oil production efficiency and CO of comparative example 3, respectively21.5, 1.2, 3.3, and 1.9 times the fixed rate. This indicates that the nitrogen-deficient, phosphorus-rich and iron-rich stage can improve the oil production efficiency and CO of the chlorella pyrenoidosa2The rate is fixed.
Example 1 compared to comparative example 1, the biomass, oil content, oil production efficiency and CO of example 12Fixed rate average ratio to biomass, oil content, oil production efficiency and CO of comparative example 12The high fixing rate shows that the phosphorus element and the iron element can synergistically improve the biomass, the oil content, the oil production efficiency and the CO of the chlorella pyrenoidosa2The rate is fixed.
The second stage culture is carried out for 12 days, namely the total days of the culture is 21 days, the biomass in the stage of nitrogen deficiency, phosphorus and iron deficiency is 6100.00mg/L, the oil content is 44.21 percent, and CO is2The carbon fixing rate is 481.85mg/L/day, and the oil production efficiency is 177.30 mg/L/day. The chlorella pyrenoidosa cultured by the method disclosed by the invention has high oil content, oil production efficiency and carbon fixation rate.
In the second stage of culture for 4 days or 12 days, that is, 13 days or 21 days, the biomass, oil content, oil production efficiency and CO of example 1 are compared with those of comparative examples 1 to 32The fixed rates are all compared with the biomass, oil content, oil production efficiency and CO of the comparative examples 1-32The high fixing rate shows that the phosphorus element and the iron element can synergistically improve the biomass, the oil content, the oil production efficiency and the CO of the chlorella pyrenoidosa2The rate is fixed.
As can be seen from Table 2, in the second stage of culture for 4 days or 12 days, i.e., 13 days or 21 days in total, the nitrogen-deficient, phosphorus-enriched and iron-enriched stage of example 1 was60The chlorella pyrenoidosa induced by CO-gamma ray is subjected to a 2-stage of nitrogen deficiency, phosphorus and iron enrichment without being subjected to60The chlorella pyrenoidosa induced by CO-gamma ray is compared with the comparative example 3 at the stage of nitrogen deficiency and phosphorus deficiency60CO-gamma ray mutagenized protein core globulesCompared with the algae, the biomass, the oil content, the carbon fixing rate and the oil production efficiency of the chlorella pyrenoidosa are higher in the example 1 and the example 2, which shows that the phosphorus element and the iron element can synergistically act to improve the biomass, the oil content, the carbon fixing rate and the oil production efficiency of the chlorella pyrenoidosa no matter whether the chlorella pyrenoidosa passes through or not60And (3) performing CO-gamma ray mutagenesis.
Claims (3)
1. A method for improving oil production efficiency and carbon sequestration rate of microalgae is characterized by comprising the following steps:
(1) inoculating microalgae in a culture medium, and performing first-stage culture; the first phase of culture is terminated when the depletion of the nitrogen and phosphorus sources in the medium is determined;
(2) after the culture of the first stage is finished, adding a phosphorus source and an iron source into a culture medium at the same time, and carrying out culture of the second stage;
the microalgae is Chlorella pyrenoidosa;
the culture in the first stage adopts BG11 culture medium, and BG11 culture medium comprises the following components: NaNO3 1500mg/L，K2HPO4·3H2O 40mg/L，MgSO4·7H2O 75mg/L，Na2CO320mg/L，CaCl227mg/L, 6mg/L citric acid monohydrate, 6mg/L ferric ammonium citrate, Na2EDTA 1mg/L, trace element A51mL of solution, wherein, A5The composition consists of the following components: h3BO3 2.86mg/L，MnCl2·4H2O 1.81mg/L，ZnSO4·7H2O 0.222mg/L，CuSO4·5H2O 0.079mg/L，CoCl2·6H2O 0.050mg/L，Na2MoO4·2H2O 0.39mg/L；
Introducing 15% by volume of CO into the culture medium2As a carbon source, the gas supply rate is 0.1 v/v/m; the temperature is 27 ℃, the pH value is 6.0-8.0, the illumination is 8500Lx, and the light dark period is 16: 8;
the phosphorus source is K2HPO4·3H2O, adding into post-culture medium PO at one time4 3-The concentration of P is 60-90 mg/L;
the iron source being FeCl3·6H2O, added in one time and added into the post-culture medium3+The concentration of (A) is 1.2-2.4 mg/L;
the culture time of the second stage is 4-12 days.
2. The method of claim 1, wherein the inoculated microalgae concentration is 400-600 mg/L.
3. The method of claim 1, wherein K is2HPO4·3H2O can be replaced by any one or a mixture of several of disodium hydrogen phosphate, sodium dihydrogen phosphate and potassium dihydrogen phosphate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810933261.7A CN110004187B (en) | 2018-08-16 | 2018-08-16 | Method for improving oil production efficiency and carbon sequestration rate of microalgae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810933261.7A CN110004187B (en) | 2018-08-16 | 2018-08-16 | Method for improving oil production efficiency and carbon sequestration rate of microalgae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110004187A CN110004187A (en) | 2019-07-12 |
| CN110004187B true CN110004187B (en) | 2021-12-28 |
Family
ID=67164822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810933261.7A Active CN110004187B (en) | 2018-08-16 | 2018-08-16 | Method for improving oil production efficiency and carbon sequestration rate of microalgae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110004187B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112175836A (en) * | 2020-10-15 | 2021-01-05 | 中国计量大学 | Method for promoting microalgae growth and oil production by using iron element in flue gas of coal-fired power plant |
| CN115029248A (en) * | 2022-06-21 | 2022-09-09 | 昆明理工大学 | Method for improving microalgae lipid yield by utilizing recycled wastewater |
| CN117736874A (en) * | 2024-01-02 | 2024-03-22 | 华北理工大学 | Chromium-containing wastewater treatment method and culture medium based on same |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268377A (en) * | 2011-07-11 | 2011-12-07 | 江南大学 | Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture |
| CN104480151A (en) * | 2014-11-20 | 2015-04-01 | 中国科学技术大学 | Method for improving yield of heterotrophic microalgal fatty acid with sodium acetate |
| CN106434778A (en) * | 2016-12-05 | 2017-02-22 | 新奥科技发展有限公司 | Method for producing grease from microalgae |
| CN106754383A (en) * | 2016-11-14 | 2017-05-31 | 华南理工大学 | A kind of method for improving microbes biomass and grease yield |
| CN107201314A (en) * | 2017-07-08 | 2017-09-26 | 东北师范大学 | It is a kind of while improving the microalgae Heterotrophic culture method of biomass and fat content |
| CN107384802A (en) * | 2017-08-23 | 2017-11-24 | 山东大学 | A kind of method for promoting microalgae grease to accumulate and keep microalgae high-biomass |
-
2018
- 2018-08-16 CN CN201810933261.7A patent/CN110004187B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102268377A (en) * | 2011-07-11 | 2011-12-07 | 江南大学 | Method for improving lipid producing microalga biomass and lipid accumulation with two stage culture strategy of mixotrophic and nitrogen-rich-nitrogen-deficient culture |
| CN104480151A (en) * | 2014-11-20 | 2015-04-01 | 中国科学技术大学 | Method for improving yield of heterotrophic microalgal fatty acid with sodium acetate |
| CN106754383A (en) * | 2016-11-14 | 2017-05-31 | 华南理工大学 | A kind of method for improving microbes biomass and grease yield |
| CN106434778A (en) * | 2016-12-05 | 2017-02-22 | 新奥科技发展有限公司 | Method for producing grease from microalgae |
| CN107201314A (en) * | 2017-07-08 | 2017-09-26 | 东北师范大学 | It is a kind of while improving the microalgae Heterotrophic culture method of biomass and fat content |
| CN107384802A (en) * | 2017-08-23 | 2017-11-24 | 山东大学 | A kind of method for promoting microalgae grease to accumulate and keep microalgae high-biomass |
Non-Patent Citations (2)
| Title |
|---|
| Effect of light, nutrient, cultivation time and salinity on lipid production of newly isolated strain of the green microalga, Botryococcus braunii KMITL 2;Suneerat Ruangsomboon et al;《Bioresour Technol》;20121231;109:261-5 * |
| 氮、磷、铁对三角褐指藻诱变株生长、总脂及脂肪酸的影响;梁晶晶等;《生态学杂志》;20161231;第35卷(第1期);189-198 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110004187A (en) | 2019-07-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yang et al. | Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii | |
| Zou et al. | Effect of light-path length in outdoor fiat plate reactors on output rate of cell mass and of EPA in Nannochloropsis sp. | |
| Feng et al. | Effects of different nitrogen sources and light paths of flat plate photobioreactors on the growth and lipid accumulation of Chlorella sp. GN1 outdoors | |
| Zhang et al. | Synergistic effects of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for enhancement of biomass and lipid yields | |
| CN110004187B (en) | Method for improving oil production efficiency and carbon sequestration rate of microalgae | |
| Chang et al. | Cultivation of Spirulina platensis for biomass production and nutrient removal from synthetic human urine | |
| Ho et al. | Engineering strategies for improving the CO2 fixation and carbohydrate productivity of Scenedesmus obliquus CNW-N used for bioethanol fermentation | |
| Münkel et al. | Optimization of outdoor cultivation in flat panel airlift reactors for lipid production by Chlorella vulgaris | |
| Zhou et al. | Evaluation of oil-producing algae as potential biodiesel feedstock | |
| CN103805514B (en) | A kind ofly photosynthetic the holding concurrently of inorganic nitrogen-sourced micro-algae is utilized to support high density fermentation cultural method and application | |
| CN109609382B (en) | Method for promoting growth of chlorella and oil accumulation by algal-bacteria co-culture | |
| CN104745513B (en) | One plant production PQQ Hyphomicrobium and its application | |
| CN109576158B (en) | Oil-rich chlorella and culture application thereof | |
| Guo et al. | Control of CO2 input conditions during outdoor culture of Chlorella vulgaris in bubble column photobioreactors | |
| CN110684667B (en) | Microalgae biofilm culture method capable of simultaneously improving biomass and grease yield | |
| CN106399111B (en) | A kind of method of the synchronous lutein and carbohydrate production for improving autotrophy microalgae | |
| Tiwari et al. | Optimization of process parameters on lipid biosynthesis for sustainable biodiesel production and evaluation of its fuel characteristics | |
| Xie et al. | Optimization of Chlorella sorokiniana cultivation condition for simultaneous enhanced biomass and lipid production via CO2 fixation | |
| CN106929550A (en) | Method for producing biodiesel raw material by using Botryococcus braunii | |
| CN114763516B (en) | Method for promoting microalgae to fix carbon and producing fatty acid by using plant hormone under mercury stress of flue gas | |
| Haixing et al. | Improvement of microalgae lipid productivity and quality in an ion-exchange-membrane photobioreactor using real municipal wastewater | |
| CN103881923A (en) | Method for culturing microalgae by using coking wastewater | |
| US20120270303A1 (en) | Manufacturing method of high content of starch from microalgae | |
| Li et al. | Numerical and experimental analysis of optimized conical flask photobioreactor structures to improve liquid–gas two-phase distribution and microalgae carbon sequestration | |
| Li et al. | Effects of simulated flue gas on components of Scenedesmus raciborskii WZKMT |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |