AU2014270410B2 - Pyrophosphorolytic sequencing - Google Patents

Pyrophosphorolytic sequencing Download PDF

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Publication number
AU2014270410B2
AU2014270410B2 AU2014270410A AU2014270410A AU2014270410B2 AU 2014270410 B2 AU2014270410 B2 AU 2014270410B2 AU 2014270410 A AU2014270410 A AU 2014270410A AU 2014270410 A AU2014270410 A AU 2014270410A AU 2014270410 B2 AU2014270410 B2 AU 2014270410B2
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AU
Australia
Prior art keywords
nucleic acid
nanopore
polymerase
target nucleic
pyrophosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2014270410A
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English (en)
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AU2014270410A1 (en
Inventor
Wouter Meuleman
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of AU2014270410A1 publication Critical patent/AU2014270410A1/en
Application granted granted Critical
Publication of AU2014270410B2 publication Critical patent/AU2014270410B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Chemical Kinetics & Catalysis (AREA)
AU2014270410A 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing Ceased AU2014270410B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361827175P 2013-05-24 2013-05-24
US61/827,175 2013-05-24
PCT/EP2014/060588 WO2014187924A1 (en) 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing

Publications (2)

Publication Number Publication Date
AU2014270410A1 AU2014270410A1 (en) 2015-10-08
AU2014270410B2 true AU2014270410B2 (en) 2020-07-16

Family

ID=50771301

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2014270410A Ceased AU2014270410B2 (en) 2013-05-24 2014-05-22 Pyrophosphorolytic sequencing

Country Status (10)

Country Link
US (4) US20140349300A1 (https=)
EP (3) EP3825415B1 (https=)
JP (1) JP6329624B2 (https=)
CN (1) CN105264093B (https=)
AU (1) AU2014270410B2 (https=)
CA (1) CA2907423A1 (https=)
DK (2) DK3004378T3 (https=)
ES (3) ES2660671T3 (https=)
PT (1) PT3004378T (https=)
WO (1) WO2014187924A1 (https=)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
JP6226869B2 (ja) 2011-10-21 2017-11-08 オックスフォード ナノポール テクノロジーズ リミテッド 酵素法
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
ES2660671T3 (es) * 2013-05-24 2018-03-23 Illumina Cambridge Limited Secuenciado pirofosforolítico usando nanoporos
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド 1つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
EP3612647B1 (en) 2017-04-19 2022-06-29 Electronic Biosciences Inc. Nanopore/nanowell electrode enabled exonuclease sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
WO2019002893A1 (en) 2017-06-30 2019-01-03 Vib Vzw NEW PROTEIN PORES
CA3118808A1 (en) 2018-11-08 2020-05-14 Oxford Nanopore Technologies Limited Pore
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
JP2022529623A (ja) 2019-04-09 2022-06-23 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
AU2020411035A1 (en) * 2019-12-23 2022-07-07 Biofidelity Ltd Kits and devices
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
US20230295712A1 (en) 2020-06-18 2023-09-21 Oxford Nanopore Technologies Plc A method of selectively characterising a polynucleotide using a detector
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
GB202118939D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Plc Pore
GB202205617D0 (en) 2022-04-14 2022-06-01 Oxford Nanopore Tech Plc Novel modified protein pores and enzymes
GB202211602D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
EP4569331A1 (en) 2022-08-09 2025-06-18 Oxford Nanopore Technologies PLC De novo pores
GB202211607D0 (en) 2022-08-09 2022-09-21 Oxford Nanopore Tech Plc Novel pore monomers and pores
WO2024033443A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
KR20250096716A (ko) 2022-10-28 2025-06-27 옥스포드 나노포어 테크놀로지즈 피엘씨 포어 단량체 및 포어
GB202216905D0 (en) 2022-11-11 2022-12-28 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202305524D0 (en) 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores
GB202307141D0 (en) 2023-05-12 2023-06-28 Oxford Nanopore Tech Plc Modified helicases
WO2025006749A1 (en) * 2023-06-30 2025-01-02 Illumina, Inc. Using apertures to capture polynucleotides on particles
GB202315935D0 (en) 2023-10-18 2023-11-29 Oxford Nanopore Tech Ltd Novel enzymes
GB202405960D0 (en) 2024-04-29 2024-06-12 Oxford Nanopore Tech Plc Photo-active conjugates and their uses
GB202413506D0 (en) 2024-09-13 2024-10-30 Oxford Nanopore Tech Plc Pore monomers and their uses

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US6107023A (en) 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
US5498523A (en) 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
EP0467953A4 (en) * 1989-04-12 1992-06-17 The President And Fellows Of Harvard College Improved primer extension reactions
US6268146B1 (en) * 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6159693A (en) * 1998-03-13 2000-12-12 Promega Corporation Nucleic acid detection
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
JP2003502166A (ja) 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
US7582490B2 (en) 1999-06-22 2009-09-01 President And Fellows Of Harvard College Controlled fabrication of gaps in electrically conducting structures
WO2001059453A2 (en) 2000-02-11 2001-08-16 The Texas A & M University System Biosensor compositions and methods of use
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US7444053B2 (en) 2003-06-16 2008-10-28 The Regents Of The University Of California Integrated electrical and optical sensor for biomolecule analysis with single molecule sensitivity
NZ549643A (en) * 2004-02-26 2009-07-31 Layerlab Aktiebolag Methods of forming lipid membrane attached linkers comprising contacting membrane with an oligonucleotide having two or more hydrophobic anchoring moieties
US7238485B2 (en) 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US7867716B2 (en) 2004-12-21 2011-01-11 The Texas A&M University System High temperature ion channels and pores
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
JP5063616B2 (ja) * 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド マイクロ流体デバイス
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB0717150D0 (en) 2007-09-04 2007-10-17 Univ Warwick Apparatus and method
ES2553893T3 (es) 2007-11-28 2015-12-14 F. Hoffmann-La Roche Ag ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CA3092369C (en) 2008-09-22 2025-09-23 University Of Washington MSP NANOPORES AND ASSOCIATED PROCESSES
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
EP2675913B1 (en) * 2011-02-15 2016-12-14 Bio-Rad Laboratories, Inc. Detecting methylation in a subpopulation of genomic dna
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
EP3385992B1 (en) * 2011-07-27 2024-09-04 The Board of Trustees of the University of Illinois Nanopore sensors for biomolecular characterization
WO2013096692A1 (en) * 2011-12-21 2013-06-27 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
GB201300957D0 (en) 2013-01-18 2013-03-06 Base4 Innovation Ltd Sequencing method
ES2660671T3 (es) * 2013-05-24 2018-03-23 Illumina Cambridge Limited Secuenciado pirofosforolítico usando nanoporos

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003080861A1 (en) * 2002-03-22 2003-10-02 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Single molecule sequencing using phosphate labeled nucleotides

Also Published As

Publication number Publication date
EP3004378A1 (en) 2016-04-13
US20200157619A1 (en) 2020-05-21
EP3351645B1 (en) 2020-07-15
ES2994071T3 (en) 2025-01-16
US20220177960A1 (en) 2022-06-09
DK3004378T3 (en) 2018-03-26
PT3004378T (pt) 2018-03-06
CN105264093A (zh) 2016-01-20
WO2014187924A1 (en) 2014-11-27
DK3351645T3 (da) 2020-09-21
HK1261919A1 (en) 2020-01-10
AU2014270410A1 (en) 2015-10-08
US20180105874A1 (en) 2018-04-19
ES2660671T3 (es) 2018-03-23
ES2817425T3 (es) 2021-04-07
US11286521B2 (en) 2022-03-29
EP3825415A1 (en) 2021-05-26
EP3351645A1 (en) 2018-07-25
CN105264093B (zh) 2018-04-10
EP3825415B1 (en) 2024-08-28
JP6329624B2 (ja) 2018-05-23
US10450605B2 (en) 2019-10-22
US20140349300A1 (en) 2014-11-27
JP2016526876A (ja) 2016-09-08
CA2907423A1 (en) 2014-11-27
EP3004378B1 (en) 2017-12-20

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NA Applications received for extensions of time, section 223

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