AU2013326786A1 - Methods and systems for microfluidics imaging and analysis - Google Patents

Methods and systems for microfluidics imaging and analysis Download PDF

Info

Publication number
AU2013326786A1
AU2013326786A1 AU2013326786A AU2013326786A AU2013326786A1 AU 2013326786 A1 AU2013326786 A1 AU 2013326786A1 AU 2013326786 A AU2013326786 A AU 2013326786A AU 2013326786 A AU2013326786 A AU 2013326786A AU 2013326786 A1 AU2013326786 A1 AU 2013326786A1
Authority
AU
Australia
Prior art keywords
sample
data
image
nucleic acid
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2013326786A
Other languages
English (en)
Inventor
Rustem F. Ismagilov
Mikhail KARYMOV
David Selck
Bing Sun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology
Original Assignee
California Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology filed Critical California Institute of Technology
Publication of AU2013326786A1 publication Critical patent/AU2013326786A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional [2D] or three-dimensional [3D] molecular structures, e.g. structural or functional relations or structure alignment
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • AIDS & HIV (AREA)
  • Optics & Photonics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AU2013326786A 2012-10-05 2013-10-04 Methods and systems for microfluidics imaging and analysis Abandoned AU2013326786A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261710454P 2012-10-05 2012-10-05
US61/710,454 2012-10-05
PCT/US2013/063594 WO2014055963A1 (en) 2012-10-05 2013-10-04 Methods and systems for microfluidics imaging and analysis

Publications (1)

Publication Number Publication Date
AU2013326786A1 true AU2013326786A1 (en) 2015-04-30

Family

ID=50435499

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013326786A Abandoned AU2013326786A1 (en) 2012-10-05 2013-10-04 Methods and systems for microfluidics imaging and analysis

Country Status (8)

Country Link
US (1) US20150247190A1 (https=)
EP (1) EP2904116A4 (https=)
JP (1) JP2016500002A (https=)
CN (1) CN104870652A (https=)
AU (1) AU2013326786A1 (https=)
CA (1) CA2887206A1 (https=)
HK (1) HK1211990A1 (https=)
WO (1) WO2014055963A1 (https=)

Families Citing this family (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US20140312247A1 (en) * 2013-04-18 2014-10-23 Bio-Rad Laboratories, Inc. Fluorescence imager on a mobile device
CA2918243A1 (en) * 2013-06-28 2014-12-31 Life Technologies Corporation Methods and systems for visualizing data quality
EP3058100A4 (en) 2013-10-18 2017-04-19 California Institute of Technology Enhanced nucleic acid identification and detection
US10260111B1 (en) 2014-01-20 2019-04-16 Brett Eric Etchebarne Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample
US20180135108A1 (en) * 2014-01-20 2018-05-17 Board Of Trustees Of Michigan State University Method for detecting bacterial and fungal pathogens
EP3594360B1 (en) 2014-04-24 2021-06-23 Lucira Health, Inc. Colorimetric detection of nucleic acid amplification
WO2016064917A1 (en) * 2014-10-21 2016-04-28 The Trustees Of Princeton University Systems and methods for personalized sample analysis
HK1243466A1 (zh) * 2014-11-05 2018-07-13 加州理工学院 生物体对药物的响应的微流体测量
US9953415B2 (en) 2014-12-03 2018-04-24 Tata Consultancy Services Limited System and method for quantification of Escherichia coli bacteria in water
WO2016094459A2 (en) * 2014-12-09 2016-06-16 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
US10998088B2 (en) 2014-12-11 2021-05-04 Hyris Limited Method for managing the protocols used by devices for diagnostic and clinical analysis and results obtained from such devices
EP4029606A1 (en) 2014-12-31 2022-07-20 Visby Medical, Inc. Molecular diagnostic testing
US20180139485A1 (en) * 2015-04-22 2018-05-17 Mohammed Tauqeer Ansari Camera System for Car Security
US10519493B2 (en) 2015-06-22 2019-12-31 Fluxergy, Llc Apparatus and method for image analysis of a fluid sample undergoing a polymerase chain reaction (PCR)
US11371091B2 (en) 2015-06-22 2022-06-28 Fluxergy, Inc. Device for analyzing a fluid sample and use of test card with same
US10214772B2 (en) 2015-06-22 2019-02-26 Fluxergy, Llc Test card for assay and method of manufacturing same
EP4227067A3 (en) * 2015-06-22 2024-01-10 FluxErgy, Inc. Device for analyzing a fluid sample and use of test card with same
WO2017025984A1 (en) * 2015-08-07 2017-02-16 Council Of Scientific And Industrial Research Smartphone integrated real - time molecular diagnostic device
WO2017079696A1 (en) * 2015-11-06 2017-05-11 California Institute Of Technology Devices and methods for direct visual detection and readout of single nucleic acid molecules
US10718003B2 (en) * 2015-12-31 2020-07-21 Roche Molecular Systems, Inc. Detecting an analyte in a flash and glow reaction
WO2017119340A1 (ja) * 2016-01-05 2017-07-13 浜松ホトニクス株式会社 生体観察方法及び生体観察装置
JP7064665B2 (ja) 2016-03-07 2022-05-11 ファーザー フラナガンズ ボーイズ ホーム ドゥーイング ビジネス アズ ボーイズ タウン ナショナル リサーチ ホスピタル 非侵襲的分子対照
EP3429543B1 (en) 2016-03-14 2024-11-20 Pfizer Inc. Selectively vented biological assay devices and associated methods
ES2927062T3 (es) 2016-03-14 2022-11-02 Lucira Health Inc Dispositivos y procedimientos para modificar propiedades ópticas
JP6949864B2 (ja) 2016-03-14 2021-10-13 ルシラ ヘルス インコーポレイテッド 生物学的アッセイ試料調製および送り出しのための装置および方法
AU2017232340B2 (en) 2016-03-14 2022-04-28 Pfizer Inc. Systems and methods for performing biological assays
WO2017197346A1 (en) * 2016-05-13 2017-11-16 Gas Sensing Technology Corp. Gross mineralogy and petrology using raman spectroscopy
CN106053467B (zh) * 2016-06-08 2019-02-19 中国科学院上海微系统与信息技术研究所 一种观测微液滴的装置及方法
FI3469338T3 (fi) * 2016-06-10 2025-05-06 Univ California Kuvapohjaisia solunlajittelujärjestelmiä ja -menetelmiä
US11168347B2 (en) 2016-09-23 2021-11-09 California Institute Of Technology Digital quantification of DNA replication and/or chromosome segregation based determination of antimicrobial susceptibility
US9857372B1 (en) * 2016-10-17 2018-01-02 Reliant Immune Diagnostics, LLC Arbovirus indicative birth defect risk test
CN106754335B (zh) * 2016-11-22 2019-05-17 上海海洋大学 一种等温核酸扩增芯片
US10218900B2 (en) * 2016-11-30 2019-02-26 Ncr Corporation Document reorientation processing
CN110268414B (zh) * 2016-12-01 2023-08-01 伯克利之光生命科技公司 微流体装置中的微物体的自动检测和重新定位
US10324041B2 (en) * 2016-12-21 2019-06-18 Abbott Japan Co., Ltd. Optical imaging system using lateral illumination for digital assays
US11047854B2 (en) 2017-02-06 2021-06-29 Abbott Japan Llc Methods for reducing noise in signal-generating digital assays
EP3615924A1 (en) * 2017-03-13 2020-03-04 Zoetis Services LLC A lateral flow test system
US10146909B2 (en) * 2017-04-06 2018-12-04 Diassess Inc. Image-based disease diagnostics using a mobile device
US11080848B2 (en) 2017-04-06 2021-08-03 Lucira Health, Inc. Image-based disease diagnostics using a mobile device
CN113777011B (zh) 2017-09-14 2024-11-19 辉瑞公司 具有电子读出的复用生物测定装置
US10549275B2 (en) 2017-09-14 2020-02-04 Lucira Health, Inc. Multiplexed biological assay device with electronic readout
US11953479B2 (en) 2017-10-06 2024-04-09 The Research Foundation For The State University Of New York Selective optical aqueous and non-aqueous detection of free sulfites
WO2019075264A1 (en) 2017-10-11 2019-04-18 California Institute Of Technology ANTIBIOSENSITIVITY OF MICROORGANISMS AND COMPOSITIONS, METHODS AND ASSOCIATED SYSTEMS
US12448655B2 (en) 2017-10-11 2025-10-21 California Institute Of Technology Antibiotic susceptibility of microorganisms and related methods and systems
SG11202002931VA (en) 2017-11-09 2020-04-29 Visby Medical Inc Portable molecular diagnostic device and methods for the detection of target viruses
WO2019169287A1 (en) * 2018-03-02 2019-09-06 Teleflex Medical Incorporated Infection detection systems and methods
EP3762708A4 (en) * 2018-03-05 2022-01-05 Mao Foodtech Ltd. SYSTEM, DEVICE AND METHOD FOR IDENTIFYING AND MONITORING A BREAST MILK COMPOSITION
US11060968B2 (en) 2018-03-30 2021-07-13 International Business Machines Corporation Mobile chemical analysis
WO2019221676A1 (en) * 2018-05-15 2019-11-21 Vivosens Bi̇yoteknoloji̇ Ar-Ge Sanayi̇ & Ti̇caret Ltd. Şti̇. Analysis of urine test strips with mobile camera analysys and providing recommendation by customising data
CN108977558A (zh) * 2018-08-24 2018-12-11 暨南大学 基于数字lamp技术检测金黄色葡萄球菌的引物及其试剂盒与方法
CN112823276A (zh) * 2018-10-09 2021-05-18 卢西拉健康公司 基于消费者的疾病诊断
USD907232S1 (en) 2018-12-21 2021-01-05 Lucira Health, Inc. Medical testing device
JP2022530323A (ja) 2019-04-28 2022-06-29 ビスビュー メディカル，インコーポレイテッド デジタル検出能力および無線接続性を有する分子診断デバイス
EP3983811A4 (en) * 2019-07-01 2022-08-17 Mantiscope Tibbi Cihazlar Arastirma ve Gelistirme Ltd. Sti. SYSTEM AND METHODS FOR DIGITALIZATION, ANALYSIS AND STORAGE OF BIOLOGICAL SAMPLES
CN110643483B (zh) * 2019-09-06 2023-04-14 上海交通大学 一种在微流控芯片上生成液滴阵列的方法
CN111197003B (zh) * 2019-12-26 2023-08-25 中国科学院合肥物质科学研究院 一种基于智能手机的集成核酸提取扩增检测的分析装置及方法
CA3170420A1 (en) * 2020-02-10 2021-08-19 Emerging Viral Diagnostics (Hk) Limited Point-of-care microfluidic in vitro diagnostic system
KR102298771B1 (ko) * 2020-02-21 2021-09-07 (주)오상헬스케어 형광염료의 종류에 따라 촬영방식을 선택하는 크로마토그래피 검사장치 및 그 제어방법
US10968493B1 (en) * 2020-03-12 2021-04-06 New England Biolabs, Inc. Rapid diagnostic test using colorimetric LAMP
US11732315B2 (en) 2020-03-12 2023-08-22 New England Biolabs, Inc. Rapid diagnostic test for lamp
US11345970B2 (en) * 2020-03-12 2022-05-31 New England Biolabs, Inc. Rapid diagnostic test for LAMP
USD953561S1 (en) 2020-05-05 2022-05-31 Lucira Health, Inc. Diagnostic device with LED display
USD962470S1 (en) 2020-06-03 2022-08-30 Lucira Health, Inc. Assay device with LCD display
US11709819B2 (en) 2020-09-30 2023-07-25 International Business Machines Corporation Validating test results using a blockchain network
US20220283088A1 (en) * 2021-02-03 2022-09-08 Joshua David Silver Viral load tester and applications thereof
CN113372559B (zh) * 2021-06-21 2023-09-15 福建立亚化学有限公司 一种液态聚碳硅烷先驱体材料的生产方法及其装置
US12275066B2 (en) 2021-10-05 2025-04-15 Nikon Corporation Systems and methods for improved melting in three-dimensional printing processes
CN114047187B (zh) * 2021-11-04 2023-10-10 温州医科大学 一种使用raw图像测量有色溶液物质浓度的方法
CN113932948B (zh) * 2021-11-15 2023-07-25 江西省永盛园艺股份有限公司 一种多肉植物种植用水分温度监测装置
US12555691B2 (en) 2022-02-22 2026-02-17 Hyperspectral Corp. Systems and methods for detecting pathogens using spectrometer scans
US12276600B2 (en) 2022-02-22 2025-04-15 Hyperspectral Corp. Systems and methods for detecting foodborne pathogens using spectral analysis
US12442768B2 (en) * 2022-09-14 2025-10-14 The Trustees Of The University Of Pennsylvania Photostimulation device and methods of using the same
TR2022017709A1 (tr) * 2022-11-23 2024-06-21 Izmir Biyotip Ve Genom Merkezi FMF (Ailevi Akdeniz Ateşi) Hastalığı Tanısı İçin Biyosensör Sistemi
CN116179321B (zh) * 2023-04-27 2023-06-23 北京慧荣和科技有限公司 气溶胶采样器以及报警器的评测装置
US11914504B1 (en) * 2023-06-27 2024-02-27 Starbucks Corporation Performing physical experiments based on automatically-generated testing scripts

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996031522A1 (en) * 1995-04-03 1996-10-10 New York University Methods for measuring physical characteristics of nucleic acids by microscopic imaging
US20020050518A1 (en) * 1997-12-08 2002-05-02 Roustaei Alexander R. Sensor array
US7640083B2 (en) * 2002-11-22 2009-12-29 Monroe David A Record and playback system for aircraft
US6522775B2 (en) * 2001-03-28 2003-02-18 Alan C. Nelson Apparatus and method for imaging small objects in a flow stream using optical tomography
WO2003100422A1 (en) * 2002-05-28 2003-12-04 Japan Genome Solutions, Inc. Dna microarray data processing method, dna microarray data processing system, dna microarray data processor, program and recording medium
EP1608952B1 (en) * 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
JP4771521B2 (ja) * 2005-08-09 2011-09-14 キヤノン株式会社 標識された核酸プライマー結合物体、その製造方法、ならびにその用途
TR201910868T4 (tr) * 2006-02-02 2019-08-21 Univ Leland Stanford Junior Dijital analizle invazif olmayan fetal genetik tarama.
EP2080025A4 (en) * 2006-10-27 2011-12-07 Montecito Bio Sciences Ltd PORTABLE DEVICE FOR IMPROVED SAMPLE ANALYSIS
CN100465990C (zh) * 2006-11-17 2009-03-04 东华大学 一种面向微流控芯片的智能定位方法
DK2291640T3 (en) * 2008-05-20 2019-03-11 Univ Health Network Device and method for fluorescence-based imaging and monitoring
WO2010053980A2 (en) * 2008-11-04 2010-05-14 The Johns Hopkins University Dna integrity assay (dia) for cancer diagnostics, using confocal fluorescence spectroscopy
US9347947B2 (en) * 2009-03-12 2016-05-24 Siemens Healthcare Diagnostics Inc. Immunoassays employing non-particulate chemiluminescent reagent
US9415392B2 (en) * 2009-03-24 2016-08-16 The University Of Chicago Slip chip device and methods
WO2010148252A1 (en) * 2009-06-17 2010-12-23 Jody Vykoukal Method and apparatus for quantitative microimaging
TW201209158A (en) * 2010-06-17 2012-03-01 Geneasys Pty Ltd LOC device for genetic analysis with dialysis, chemical lysis and tandem nucleic acid amplification
CN102004161B (zh) * 2010-11-09 2012-08-15 华中科技大学 一种微阵列反应装置
GB201105474D0 (en) * 2011-03-31 2011-05-18 Albagaia Ltd Testing apparatus
CN104630373B (zh) * 2015-02-13 2017-11-21 博奥生物集团有限公司 一种微流控芯片核酸快速并行检测方法及系统

Also Published As

Publication number Publication date
HK1211990A1 (en) 2016-06-03
US20150247190A1 (en) 2015-09-03
JP2016500002A (ja) 2016-01-07
CN104870652A (zh) 2015-08-26
EP2904116A1 (en) 2015-08-12
WO2014055963A1 (en) 2014-04-10
CA2887206A1 (en) 2014-04-10
EP2904116A4 (en) 2016-05-11

Similar Documents

Publication Publication Date Title
US20150247190A1 (en) Methods and systems for microfluidics imaging and analysis
Selck et al. Increased robustness of single-molecule counting with microfluidics, digital isothermal amplification, and a mobile phone versus real-time kinetic measurements
US12400733B2 (en) In situ code design methods for minimizing optical crowding
Trieu et al. based all-in-one origami microdevice for nucleic acid amplification testing for rapid colorimetric identification of live cells for point-of-care testing
Rodriguez-Manzano et al. Reading out single-molecule digital RNA and DNA isothermal amplification in nanoliter volumes with unmodified camera phones
CN109563462B (zh) 检测特定核酸序列的完全集成的手持式设备
US10866184B2 (en) Devices and methods for direct visual detection and readout of single nucleic acid molecules
EP4189108A1 (en) Multiplexed covid-19 padlock assay
US20190234978A1 (en) System for nucleic acid preparation
US20140211204A1 (en) Hand-held wireless platform and optics for measurement of dna, rna, micrornas, and other markers of pathogens, genetic diseases, and cancer
US20160319354A1 (en) Systems, compositions and methods for detecting and analyzing micro-rna profiles from a biological sample
TW201211540A (en) LOC device for pathogen detection and genetic analysis with dialysis and nucleic acid amplification
Yee et al. Polymerization-based amplification for target-specific colorimetric detection of amplified Mycobacterium tuberculosis DNA on cellulose
Robin et al. A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus
Wang et al. Amine-functionalized quantum dots as a universal fluorescent nanoprobe for a one-step loop-mediated isothermal amplification assay with single-copy sensitivity
Dong et al. A Portable Nucleic Acid Testing Platform with Photosensitization, a Three-Dimensionally Printed Multipiece Chip, and Digital Color Sensing
KR20220167379A (ko) 핵산 검출을 위한 하이-플렉스 가이드 풀링
WO2016004155A1 (en) Devices and methods for monitoring and quantifying nucleic acid amplification
WO2018156906A1 (en) System and method for purifying and amplifying nucleic acids
WO2025014758A9 (en) Systems and methods for tissue bounds detection
US12435357B2 (en) Diagnostic device for detecting a target nucleic acid molecule in a biological sample
EP4646594A1 (en) Camouflaging on chip, methods, and systems and its use for testing biological and non-biological analytes
WO2025122890A1 (en) Graphical user interface and method of estimating an instrument run completion time
Stedtfeld Development and validation of a multiplex hand-held gene analyzer
Chen Smartphone-based systems for mobile infectious disease detection and epidemiology

Legal Events

Date Code Title Description
MK1 Application lapsed section 142(2)(a) - no request for examination in relevant period