AU2013202998B2

AU2013202998B2 - Stable and soluble antibodies inhibiting vegf

Info

Publication number: AU2013202998B2
Application number: AU2013202998A
Authority: AU
Inventors: Leonardo Borras; Tea Gunde; David Urech
Original assignee: Novartis AG
Current assignee: Novartis AG
Priority date: 2008-06-25
Filing date: 2013-04-08
Publication date: 2015-02-26
Also published as: AU2013202998A1

Abstract

The present invention relates to soluble and stable anti-VEGF imunobinders comprising CDRs from rabbit monoclonal antibodies. Said antibodies are designed for the diagnosis and/or treatment of VEGF-mediated disorders. The hybridomas, nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, methods for isolating them and the use of said antibodies in medicine are also disclosed.

Description

I STABLE AND SOLUBLE ANTIBODIES INHIBITING VEGF The present application is a divisional application from Australian patent application number 2009264565, the entire disclosure of which is incorporated herein by reference. Background of the Invention Angiogenesis is implicated in the pathogenesis of a variety of disorders including solid tumors, intraocular neovascular syndromes such as proliferative retinopathies or age related macular degeneration (AMD), rheumatoid arthritis, and psoriasis (Folkman et al. J. Biol. Chem. 267:10931-10934 (1992); Klagsbrun et al. Annu. R.ev. Physiol. 53:217239 (1991); and Garner A, Vascular diseases. In: Pathobiology of ocular disease. A dynamic approach. Garner A, Klintworth G K, Eds. 2nd Edition Marcel Dekker, NY, pp 1625-1710 (1994)). In solid tumors, angiogenesis and growth of new vasculture permits survival of the tumor, and a correlation has been demonstrated between the density of microvessels in tumor sections and patient survival in breast and other cancers (Weidner et al. N EngI J Med 324:1 6(1991); Horak et al. Lancet 340:1120-1124 (1992); and Macchiarini et al. Lancet 340:145 146 (1992)). Vascular endothelial growth factor (VEGF) is a known regulator of angiogenesis and neovascularization, and has been shown to be a key mediator of neovascularization associated with tumors and intraocular disorders (Ferrara et al. Endocr. Rev. 18:4-25 (1997)). The VEGF mnRNA is overexpressed in iany human tumors, and the concentration of VEGF in eye fluids are highly correlated to the presence of active proliferation of blood vessels in patients with diabetic and other ischemia-related retinopathies (Berkman et al., J Clin Invest 91:153-159 (1993); Brown et al. Human Pathol. 26:86-91 (1995); Brown et al. Cancer Res. 53:4727-4735 (1993); Mattem et al. Brit. J. Cancer. 73:931-934 (1996); and Dvorak et al. Am J. Pathol. 146:1029-1039 (1995); Aiello et al. N. Engl. J. Med. 331:1480-1487 (1994)). In addition, recent studies 2 have shown the presence of localized VEGF in choroidal neovascular membranes in patients affected by AMD (Lopez et al. Invest. Ophtalmo. Vis. Sci. 37:855-868 (1996)). Anti-VEGF neutralizing antibodies can be used to suppress the growth of a variety of human tumor cell lines in nude mice and also inhibit intraocular angiogenesis in models of ischemic retinal disorders (Kim et al. Nature 362:841-844 (1993); Warren et al. J. Clin. Invest 95:1789-1797 (1995); Borgstrom et al. Cancer Res. 56:4032-4039 (1996); and Melnyk et al. Cancer Res. 56:921-924 (1996)) (Adamis et al. Arch. Opthalmol. 114:66-71 (1996)). Thus, there is a need for anti-VEGF monoclonal antibodies capable of being used for the treatment of solid tumors and various neovascular intraocular diseases. Summary of the Invention The invention provides a recombinant antibody comprising a variable heavy chain (VH) and a variable light chain (VL), wherein the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 164 and the light chain variable region comprises the amino acid sequence of SEQ ID NO: 87. The invention further provides soluble and stable anti-VEGF immunobinders comprising CDRs from rabbit monoclonal antibodies. Said antibodies are designed for the diagnosis and/or treatment of VEGF-mediated disorders. The hybridomas, nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, methods for isolating them and the use of said antibodies in medicine are also disclosed. Brief Description of the Drawings Figure 1 illustrates the binding kinetics of selected scFvs to hVEGF 165 using Biacore (hVEGF 165). Fig. la shows the data obtained for 511 max: Ka (1/Ms): 6,59E+05; SE (ka): 1,10E+03; kd(1/s):4,40E-05; SE(kd):6,30E-07; KD(M): 6,67E-11. Fig. lb shows the data obtained for 578max: Ka (1/Ms): 7,OOE+05; SE (ka): 1,40E+03; kd(l/s): 3,07E-04; SE(kd): 8,50E-07; KD(M): 4,39E-10. Figure 2 illustrates the species specificity by showing binding kinetics of 578max to human, mouse and rat VEGF. Fig. 2a shows the data obtained for human VEGF 165: Ka (1/Ms): 7,OOE+05; SE (ka): 1,40E+03; kd(1/s): 3,07E-04; SE(kd): 8,50E-07; KD(M): 4,39E-10. Fig. 2b 2a shows the data obtained for mouse VEGF164: Ka (1/Ms): 1,03E+06; SE (ka): 2,30E+03; kd(1/s): 4,40E-04; SE(kd): 9,40E-07; KD(M): 4,29E-10. Fig. 2c shows the data obtained for rat VEGF 164: Ka (1/Ms): 8,83E+05; SE (ka): 2,50E+03; kd(1/s): 5,28E-04; SE(kd): 1,20E-06; KD(M): 5,98E-10.

WO 2009/155724 PCT/CH2009/000220 3 Figure 3 illustrates the binding kinetics of 578max toVEGF isoforms (hVEGF121 and hVEGFI 10). Fig. 3a shows the data obtained for human VEGF 165: Ka (l/Ms): 7,00E+05; SE (ka): 1,4E+03; kd(1/s): 3,07E-04; SE(kd): 8.50E-07; KD(M): 4,39E-10. Fig. 3b shows the data obtained for human VEGF 121: Ka (i/Ms): 5,87E+05; SE (ka): 5 1,20E+03; kd(1/s): 5,58E-04; SE(kd): 9,60E-07; K.D(M): 9,50E-1 1. Fig. 3c shows the data obtained for human VEGFI 10: Ka (I/Ms): 5,23E+05; SE (ka): 1,30E+03; kd(l /s): 7,22E-04; SE(kd): 8,10E-07; KD(M): 1,38E-09. Figure 4 depicts the binding kinetics of 578max, 578minmax and 578wt to hVEGF 165. Fig. 4a shows the data obtained for 578max: Ka (l/Ms): 7,00E+05; SE (ka): 10 1,40E+03; kd(1/s): 3,07E-04; SE(kd): 8,50E-07; KD(M): 4,39E-10. Fig. 4b shows the data obtained for 578minmax: Ka (1/Ms): 8,06E+05; SE (ka): 2,1OE+03; kd(l/s): 5,04E 04; SE(kd): 1,10E-06; KD(M): 6,25E-10. Fig. 4c shows the data obtained for 578wt-His: Ka (1/Ms): 8,45E+05; SE (ka): 1,60E+03; kd(l/s): 1,69E-04; SE(kd): 7,60E-07; KD(M): 2,OOE- 10. I5 Figure 5 illustrates thermal stability of 578max, 578minmax and 578minmaxDHP (unfolding measured by FT-IR). Fig. Sa: 578minmax (E3SBA903): Tm = 71,1*C; Fig. 5b: 578minmaxDHP (#961): Tm=70,2*C; Fig. Sc: 578max (#821): Tm 70,4-C. Figure 6 illustrates denaturation and precipitation of 578 derivatives after thermal 20 stress (Fig 6a: 50*C, Fig 6b: 60*C, Fig 6c: 70'C) for 30 min. Figure 7 illustrates solubility of 578max, 578minmax and 578minmax DHP (determined by ammonium sulfate precipitation). Fig 7a: 578max (#821). The V50 was 27,24 % Fig. 7b: 578minmax (ESBA903). The V50 was 28,13. Fig.: 7c: 578minmaxDIP (#961). The V50was 32,36 %. 25 Figure 8 illustrates VEGFR2 competition ELISA versus HUVEC assay as methods to measure potency. Fig. 8a: Comparison of Lucentis and 511 max (#802) in VEGFR2 competition ELISA. R 2 of Lucentis: 0,9417; R2 of ESBA802: 0,9700. EC50 of Lucentis: 7,137 nM; EC50 of #802: 0,8221 nM. Fig 8b: Comparison of Lucentis and 578max (#821). in VEGFR2 competition ELISA. Fig 8c: Comparison of Lucentis, 30 511 maxC-his and 534max in 1-UVEC assay. R 2 of Lucentis 0,9399; R2 of EP5I IrnaxC his: 0,9313, R 2 of EP534max: 0,7391. EC50 of Lucentis: 0,08825 nM, EC50 of WO 2009/155724 PCT/CH2009/000220 4 511 maxC-his: 0,7646 nM, EC50 of 534max: 63,49 nM, Fig. 8d: Comparison of Lucentis, 578min and 578max in HUVEC assay. R 2 of Lucentis: 0,9419, R 2 of EP578min: 0,8886, R2 of EP578max: 0,9274. EC50 of Lucentis: 0,1529 nM, EC50 of 578min: 1,528 nM, EC50 of 578max: 0,1031 nM. 5 Figure 9 illustrates the effects of 578minmax on HUVEC proliferation induced by hVEGF165. The parameters of the assay were the following: hVEGF165 concentration: 0,08nM (3ng/ml); incubation with VEGF and test item: 96h. The EC50 was 0,08959 nM for Lucentis and 0,05516 nM for 578minmax, whereas the R 2 was 0,9066 for Lucentis and 0,9622 for 578minmax. 10 Figure 10 illustrates the effects of 578minmax on HUVEC Proliferation induced by mouse VEGF 164 and rat VEGF 164. The parameters of the assay were the following: mouse VEGF 164 concentration: 0;08nM-(3ng/ml); rat VEGF 164 concentration: 0,3nM (11,3ng/ml), Both concentrations were selected at EC90 for VEGF induced HUVEC proliferation; incubation with VEGF and test item: 96h. Fig. 10a illustrates the data 15 obtained for mouse VEGF. The EC50 was 0,1196 nM for V1253 and 0,06309 nM for 578minmax, whereas the R2 was 0,02744 for Lucentis, 0,9348 for V1253 and 0,9767 for EP578minmax. Lucentis did not inhibit HIUVEC proliferation induced by mouse VEGF. Fig. l0b illustrates the data obtained for rat VEGF. The EC50 was 1,597 nM for V1253 and 0,06974 nM for 578minmax, whereas the R2 was 00,7664 for V1253 and 0,6635 for 20 578minmax. Figure 11 illustrates efficacy studies using Miles assay in nude guinea pigs (part I). The dye almar blue I was administered intravenously to nude guinea pigs. One hour after dye injection, a premixture 2 of hVEGF (2,61nM) and Lucentis, ESBA903 or #802, respectively, was injected into the skin of the animal 3. One hour after injection of the 25 solutions, the animals 3 were euthanized and the pelts were collected, cleaned and photographed digitally using incident and transmitted light. The area of Evans Blue dye that extravasated into the injection sites was evaluated using Image J and the dose-area retention was plotted. Figure 12 illustrates efficacy studies using Miles assay in nude guinea pigs (part 30 II). Fig. 12a shows the results obtained for #803 (51 Imax). The EC50 was 5,990nM and had a statistical spread between 2,060 and 17,41 nM whereas the R 2 was 0,5800. Fig. 12b WO 2009/155724 PCT/CH2009/000220 5 shows the results obtained for ESBA903 (578minmax). The EC50 was 3,989 and had a statistical spread between 1,456 and 10,93 nM whereas the R 2 was 0,3920. Fig. 12c shows the area of dye leakage for Lucentis. The EC50 could not be calculated for Lucentis due to the poor fit of the curve. 5 Figure 13 illustrates efficacy studies using modified miles assay in rats (premixed hVEGF 165 and 578minmax (ESBA903)). Fig. 13a illustrates the anti-permeability efficacy of Avastin upon VEGF induced retinal vascular leakage in rats - dose response. Avastin inhibits hVEGF-induced retinal vascular permeability. Premixed before injection. Approximately equimolar, 3fold, or 10 fold excess. *p<0,05 (VEGF s. BSA), 10 ** p< 0

,

0 5 (Avastin treated vs. VEGF). Fig l3b shows the anti-permeability efficacy of ESBA903 upon VEGF induced retinal vascular leakage in rats. Dose response (pre mixed, ivt). Complete inhibition of hVEGF-induced-retinal vascular permeability by ESBA903. Premixed before injection. Approximately equimolar, 3fold, or 10 fold excess. *p<0,05 (VEGF s. BSA), ** p<0,05 (ESBA903 treated vs. VEGF). 15 Figure 14 illustrates efficacy studies using modified miles assay in rats (topical administration of 578minmax (ESBA903)). The anti-permeability efficacy of AL-51287 (ESBA903) upon VEGF induced retinal vascular leakage in rats was tested upon topical administration. Five days pretreatment, 4 drops/day with a 10 ng/ml ESBA903 formulation. *p<0,05 (VEGF s. BSA), ** p<0,05 (VEGF vs. AL-51287), ***p::0,060 20 (AL-51287 vs. AL-52667), ****(VEGF vs. AL-39324); p<0,05 (AL-39324 vs. vehicle ref ctrl). AL-51287: ESBA903; AL-52657: topical vehicle reference control; AL-39324: small molecule RTK inhibitor. Figure 15 illustrates the definition of CDRI of VH as used herein. 25 Detailed Description The invention provides soluble and stable anti-VEGF imunobinders comprising CDRs from rabbit monoclonal antibodies. Said imunobinders are designed for the diagnosis and/or treatment of VEGF-mediated disorders. The hybridomas, nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, 30 methods for isolating them and the use of said antibodies in medicine are also disclosed.

WO 2009/155724 PCT/CH2009/000220 6 Definitions In order that the present invention may be more readily understood, certain terms will be defined as follows. Additional definitions are set forth throughout the detailed description. The term "VEGF" refers to the 165-amino acid vascular endothelial cell growth factor, and related 121-, 189-, and 206-amino acid vascular endothelial cell growth factors, as described by Leung et al., Science 246:1306 (1989), and Houck et al., Mol. Endocrin. 5:1806 (1991) together with the naturally occurring allelic and processed forms of those growth factors. 10 The term "VEGF receptor" or "VEGFr" refers to a cellular receptor for VEGF, ordinarily a cell-surface receptor found on vascular endothelial cells, as well as variants thereof which retain the ability to bind hVEGF. One example of a VEGF receptor is the fins-like tyrosine kinase (fit), a transmembrane receptor in the tyrosine kinase family. DeVries et al., Science 255:989 (1992); Shibuya et al., Oncogene 5:519 (1990). The fit 15 receptor comprises an extracellular domain, a transmembrane domain, and an intracellular domain with tyrosine kinase activity. The extracellular domain is involved in the binding of VEGF, whereas the intracellular domain is involved in signal transduction. Another example of a VEGF receptor is the Ilk-I receptor (also referred to as KDR). Matthews et al., Proc. Nat. Acad. Sci. 88:9026 (1991); Terman et al., Oncogene 6:1677 20 (1991); Terman et al., Biochem. Biophys. Res. Commun. 187:1579 (1992). Binding of VEGF to the fit receptor results in the formation of at least two high molecular weight complexes, having an apparent molecular weight of 205,000 and 300,000 Daltons. The 300,000 Dalton complex is believed to be a dimer comprising two receptor molecules bound to a single molecule of VEGF. 25 The term "rabbit" as used herein refers to an animal belonging to the family of the leporidae. The term "antibody" as used herein is a synonym for "immunoglobulin." Antibodies according to the present invention may be whole immunoglobulins or fragments thereof, comprising at least one variable domain of an immunoglobulin, such 30 as single variable domains, Fv (Skerra A. and Pluckthun, A. (1988) Science 240:1038 41), scFv (Bird, R.E. et al. (1988) Science 242:423-26; Huston, J.S. et al. (1988) Proc.

WO 2009/155724 PCT/CH2009/000220 7 Nati A cad. Sci. USA 85:5879-83), Fab, (Fab')2 or other fragments well known to a person skilled in the art. The term " CDR" refers to one of the six hypervariable regions within the variable domains of an antibody that mainly contribute to antigen binding. One of the most 5 commonly used definitions for the six CDRs was provided by Kabat E.A. et aL, (1991) Sequences of proteins of immunological interest. NIH Publication 91-3242). As used herein, Kabat's definition of CDRs only apply for CDR1, CDR2 and CDR3 of the light chain variable domain (CDR L1, CDR L2, CDR L3, or L1, L2, L3), as well as for CDR2 and CDR3 of the heavy chain variable domain (CDR H2, CDR H3, or H2, H3). CDRI of 10 the heavy chain variable domain (CDR H1 or H1), however, as used herein is defined by the following residues (Kabat numbering): It starts with position 26 and ends prior to position 36. Thisis basically a fusion of CDR H1 as differently defined by-Kabat and Chotia (see also Figure 15 for illustration). The term "antibody framework", or sometimes only "framework", as used herein 1 5 refers to the part of the variable domain, either VL or VH, which serves as a scaffold for the antigen binding loops (CDRs) of this variable domain. In essence it is the variable domain without the CDRs. The term "single chain antibody", "single chain Fv" or "scFv" is intended to refer to a molecule comprising an antibody heavy chain variable domain (or region; VH) and 20 an antibody light chain variable domain (or region; VL) connected by a linker. Such scFv molecules can have the general structures: NH 2 -VL-linker-VH-COOH or NH 2 V-linker VL-COOH. As used herein, "identity" refers to the sequence matching between two polypeptides, molecules or between two nucleic acids. When a position in both of the 25 two compared sequences is occupied by the same base or amino acid monomer subunit (for instance, if a position in each of the two DNA molecules is occupied by adenine, or a position in each of two polypeptides is occupied by a lysine), then the respective molecules are identical at that position. The "percentage identity" between two sequences is a function of the number of matching positions shared by the two sequences 30 divided by the number of positions compared x 100. For instance, if 6 of 10 of the positions in two sequences are matched, then the two sequences have 60% identity. By WO 2009/155724 PCT/CH2009/000220 8 way of example, the DNA sequences CTGACT and CAGGTT share 50% identity (3 of the 6 total positions are matched). Generally, a comparison is made when two sequences are aligned to give maximum identity. Such alignment can be provided using, for instance, the method of Needleman et al (1970) J Mo. BioL 48: 443-453, implemented 5 conveniently by computer programs such as the Align program (DNAstar, Inc.). The percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Apple. Biosci., 4:11-17 (1988)) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4. In addition, the percent 10 identity between two amino acid sequences can be determined using the Needleman and Wunsch (J. Mol. Biol. 48:444-453 (1970)) algorithm which has been incorporated into the GAP program in the GCGisoftvare package (available at www.gcg.com), using either,-.. a Blossum 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. 15 "Similar" sequences are those which, when aligned, share identical and similar amino acid residues, where similar residues are conservative substitutions for corresponding amino acid residues in an aligned reference sequence. In this regard, a "conservative substitution" of a residue in a reference sequence is a substitution by a residue that is physically or functionally similar to the corresponding reference residue, 20 e.g., that has a similar size, shape, electric charge, chemical properties, including the ability to form covalent or hydrogen bonds, or the like. Thus, a "conservative substitution modified" sequence is one that differs from a reference sequence or a wild type sequence in that one or more conservative substitutions are present. The "percentage similarity" between two sequences is a function of the number of positions 25 that contain matching residues or conservative substitutions shared by the two sequences divided by the number of positions compared x 100. For instance, if 6 of 10 of the positions in two sequences are matched and 2 of 10 positions contain conservative substitutions, then the two sequences have 80% positive similarity. As used herein, the term "conservative sequence modifications" is intended to 30 refer to amino acid modifications that do not negatively affect or alter the binding characteristics of the antibody containing the amino acid sequence. Such conservative WO 2009/155724 PCT/CH2009/000220 9 sequence modifications include nucleotide and amino acid substitutions, additions and deletions. For example, modifications can be introduced by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis. Conservative amino acid substitutions include ones in which the amino acid residue is 5 replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g., lysine, arginine, histidine), acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polar side chains (e.g., glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan), nonpolar side chains (e.g., 10 alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine), beta-branched side chains (e.g., threonine, valine, isoleucine) and aromatic side chains (e.g., tyrosine, phenylalanine, tryptophan, histidine). Thus a predicted nonessential amino acid residue in a human anti-VEGF antibody is preferably replaced with another amino acid residue from the same side chain family. Methods of identifying nucleotide and amino acid 15 conservative substitutions which do not eliminate antigen binding are well-known in the art (see, e.g., Brummell et al., Biochem. 32:1180-1187 (1993); Kobayashi et al. Protein Eng. 12(10):879-884 (1999); and Burks et al. Proc. Nat. Acad. Sci. USA 94:412-417 (1997)) "Amino acid consensus sequence" as used herein refers to an amino acid sequence 20 that can be generated using a matrix of at least two, and preferably more, aligned amino acid sequences, and allowing for gaps in the alignment, such that it is possible to determine the most frequent amino acid residue at each position. The consensus sequence is that sequence which comprises the amino acids which are most frequently represented at each position. In the event that two or more amino acids are equally 25 represented at a single position, the consensus sequence includes both or all of those amino acids. The amino acid sequence of a protein can be analyzed at various levels. For example, conservation or variability can be exhibited at the single residue level, multiple residue level, multiple residue with gaps etc. Residues can exhibit conservation of the 30 identical residue or can be conserved at the class level. Examples of amino acid classes include polar but uncharged R groups (Serine, Threonine, Asparagine and Glutamine); WO 2009/155724 PCT/CH2009/000220 10 positively charged R groups (Lysine, Arginine, and Histidine); negatively charged R groups (Glutamic acid and Aspartic acid); hydrophobic R groups (Alanine, Isoleucine, Leucine, Methionine, Phenylalanine, Tryptophan, Valine and Tyrosine); and special amino acids (Cysteine, Glycine and Proline). Other classes are known to one of skill in 5 the art and may be defined using structural determinations or other data to assess substitutability. In that sense, a substitutable amino acid can refer to any amino acid which can be substituted and maintain functional conservation at that position. It will be recognized, however, that amino acids of the same class may vary in degree by their biophysical properties. For example, it will be recognized that certain 10 hydrophobic R groups (e.g., Alanine, Serine, or Threonine) are more hydrophilic (i.e., of higher hydrophilicity or lower hydrophobicity) than other hydrophobic R groups (e.g., Valine or Leucine). Relative hydrophilicity or hydrophobicity -can be determined using art-recognized methods (see, e.g., Rose et aL., Science, 229: 834-838 (1985) and Cornette et al., J. Mol. Biol., 195: 659-685 (1987)). 15 As used herein, when one amino acid sequence (e.g., a first VH or VL sequence) is aligned with one or more additional amino acid sequences (e.g., one or more VH or VL sequences in a database), an amino acid position in one sequence (e.g., the first Vs or VL sequence) can be compared to a "corresponding position" in the one or more additional amino acid sequences. As used herein, the "corresponding position" represents the 20 equivalent position in the sequence(s) being compared when the sequences are optimally aligned, i.e., when the sequences are aligned to achieve the highest percent identity or percent similarity. As used herein, the term "antibody database" refers to a collection of two or more antibody amino acid sequences (a "multiplicity" of sequences), and typically refers to a 25 collection of tens, hundreds or even thousands of antibody amino acid sequences. An antibody database can store amino acid sequences of, for example, a collection of antibody VH regions, antibody VL regions or both, or can store a collection of scFv sequences comprised of VH and VL regions. Preferably, the database is stored in a searchable, fixed medium, such as on a computer within a searchable computer program. 30 In one embodiment, the antibody database is a database comprising or consisting of germline antibody sequences. In another embodiment, the antibody database is a WO 2009/155724 PCT/CH2009/000220 11 database comprising or consisting of mature (Le., expressed) antibody sequences (e.g., a Kabat database of mature antibody sequences, e.g., a KBD database). In yet another embodiment, the antibody database comprises or consists of functionally selected sequences (e.g., sequences selected from a QC assay). 5 The term "immunobinder" refers to a molecule that contains all or a part of the antigen binding site of an antibody, e.g., all or part of the heavy and/or light chain variable domain, such that the immunobinder specifically recognizes a target antigen. Non-limiting examples of immunobinders include full-length immunoglobulin molecules and scFvs, as well as antibody fragments, including but not limited to (i) a Fab fragment, 10 a monovalent fragment consisting of the VL, VH, CL and CH I domains; (ii) a F(ab') 2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fab' fragment, which is essentially a Fab with-part of the hinge region (see, Fundamental Immunology (Paul ed., 3.sup.rd ed. 1993); (iv) a Fd fragment consisting of the VH and CHil domains; (v) a Fv fragment consisting of the VL and VH 1 15 domains of a single arm of an antibody, (vi) a single domain antibody such as a Dab fragment (Ward et al., (1989),Nature 341:544-546), which consists of a VH or VL domain, a Camelid (see Hamers-Casterman, et aL, Nature 363:446-448 (1993), and Dumoulin, et aL, Protein Science 11:500-515 (2002)) or a Shark antibody (e.g., shark Ig NARs Nanobodies@); and (vii) a nanobody, a heavy chain region containing the variable 20 domain and two constant domains. As used herein, the term "functional property" is a property of a polypeptide (e.g., an immunobinder) for which an improvement (e.g., relative to a conventional polypeptide) is desirable and/or advantageous to one of skill in the art, e.g., in order to improve the manufacturing properties or therapeutic efficacy of the polypeptide. In one 25 embodiment, the functional property is stability (e.g., thermal stability). In another embodiment, the functional property is solubility (e.g., under cellular conditions). In yet another embodiment, the functional property is non-aggregation. In still another embodiment, the functional property is protein expression (e.g., in a prokaryotic cell). In yet another embodiment the functional property is a refolding efficiency following an 30 inclusion body solubilization in a corresponding purification process. In certain WO 2009/155724 PCT/CH2009/000220 12 embodiments, antigen binding affinity is not a functional property desired for improvement. The term "epitope" or "antigenic determinant" refers to a site on an antigen (e.g., on VEGF) to which an immunoglobulin or antibody specifically binds. An epitope 5 typically includes at least 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 consecutive or non consecutive amino acids in a unique spatial conformation. See, e.g., Epitope Mapping Protocols in Methods in Molecular Biology, Vol. 66, G. E. Morris, Ed. (1996). The terms "specific binding," "selective binding," "selectively binds," and "specifically binds," refer to antibody binding to an epitope on a predetermined antigen. 10 Typically, the antibody binds with an affinity (KD) of approximately less than 107 M, such as approximately less than 10 8 M, 10- M or IWO M or even lower. The term "KD;" f"Kd"-refers to the dissociation equilibrium constant of particular antibody-antigen interaction. Typically, the antibodies of the invention bind to VEGF with a dissociation equilibrium constant (KD) of less than approximately 107 M, 15 such as less than approximately 104 M, 10- M or 10- 0 M or even lower, for example, as determined using surface plasmon resonance (SPR) technology in a BIACORE instrument. The terms "neutralizes VEGF," "inhibits VEGF," and "blocks VEGF" are used interchangeably to refer to the ability of an antibody of the invention to prevent VEGF 20 from interacting with one or more VEGF receptors such as VEGFR-1I and/or VEGFR-2, and, for example, triggering signal transduction. A "recombinant immunobinder" as used herein refers to an immunobinder being produced by expression from recombinant DNA. A "chimeric" immunobinder as used herein has a portion of the heavy and/or light 25 chain identical with or homologous to corresponding sequences in antibodies derived from a particular species or belonging to a particular antibody class or subclass, while the remainder of the chain(s) is identical with or homologous to corresponding sequences in antibodies derived from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies. Humanized antibody as used herein is a 30 subset of chimeric antibodies.

WO 2009/155724 PCT/CH2009/000220 13 "Humanized antibodies" as used herein are immunobinders that have been synthesized using recombinant DNA technology to circumvent immune response to foreign antigens. Humanization is a well-established technique for reducing the immunogenicity of monoclonal antibodies of xenogenic sources. This involves the choice 5 of an acceptor framework, preferably a human acceptor framework, the extent of the CDRs from the donor immunobinder to be inserted into the acceptor framework and the substitution of residues from the donor framework into the acceptor framework. A general method for grafting CDRs into human acceptor frameworks has been disclosed by Winter in US Patent No. 5,225,539, which is hereby incorporated by reference in its 10 entirety. US6,407,213 the teachings of which are incorporated by reference in its entirety, discloses a number of amino acid positions of the framework where a substitution from the donor immunobinder is preferred,- The term "nucleic acid molecule," refers to DNA molecules and RNA molecules. A nucleic acid molecule may be single-stranded or double-stranded, but preferably is 15 double-stranded DNA, A nucleic acid is "operably linked" when it is placed into a functional relationship with another nucleic acid sequence. For instance, a promoter or enhancer is operably linked to a coding sequence if it affects the transcription of the sequence. The term "vector," refers to a nucleic acid molecule capable of transporting 20 another nucleic acid to which it has been linked. One type of vector is a "plasmid," which refers to a circular double stranded DNA loop into which additional DNA segments may be ligated. Another type of vector is a viral vector, wherein additional DNA segments may be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g., bacterial 25 vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) can be integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. The term "host cell" refers to a cell into which an expression vector has been 30 introduced. Host cells can include bacterial, microbial, plant or animal cells. Bacteria, which are susceptible to transformation, include members of the enterobacteriaceae, such WO 2009/155724 PCT/CH2009/000220 14 as strains of Escherichia coli or Salmonella; Bacillaceae, such as Bacillus subtilis; Pneumococcus; Streptococcus, and Haemophilus influenzae. Suitable microbes include Saccharomyces cerevisiae and Pichiapastoris. Suitable animal host cell lines include CHO (Chinese Hamster Ovary lines) and NSO cells. 5 The terms "treat," "treating," and "treatment," refer to therapeutic or preventative measures described herein. The methods of "treatment" employ administration to a subject, in need of such treatment, an antibody of the present invention, for example, a subject having a VEGF-mediated disorder or a subject who ultimately may acquire such a disorder, in order to prevent, cure, delay, reduce the severity of, or ameliorate one or 10 more symptoms of the disorder or recurring disorder, or in order to prolong the survival of a subject beyond that expected in the absence of such treatment. The term "VEGF-mediated disorder" refers to -any disorder, the onset, progression or the persistence of the symptoms or disease states of which requires the participation of VEGF. Exemplary VEGF-mediated disorders include, but are not limited 15 to, age-related macular degeneration, neovascular glaucoma, diabetic retinopathy, retinopathy of prematurity, retrolental fibroplasia, breast carcinomas, lung carcinomas, gastric carcinomas, esophageal carcinomas, colorectal carcinomas, liver carcinomas, ovarian carcinomas, the comas, arrhenoblastomas, cervical carcinomas, endometrial carcinoma, endometrial hyperplasia, endometriosis, fibrosarcomas, choriocarcinoma, 20 head and neck cancer, nasopharyngeal carcinoma, laryngeal carcinomas, hepatoblastoma, Kaposi's sarcoma, melanoma, skin carcinomas, hemangioma, cavernous hemangioma, hemangioblastoma, pancreas carcinomas, retinoblastoma, astrocytoma, glioblastoma, Schwannoma, oligodendroglioma, medulloblastoma, neuroblastomas, rhabdomyosarcoma, osteogenic sarcoma, leiomyosarcomas, urinary tract carcinomas, 25 thyroid carcinomas, Wilm's tumor, renal cell carcinoma, prostate carcinoma, abnormal vascular proliferation associated with phakomatoses, edema (such as that associated with brain tumors), Meigs' syndrome, rheumatoid arthritis, psoriasis and atherosclerosis. The term "effective dose" or "effective dosage" refers to an amount sufficient to achieve or at least partially achieve the desired effect. The term "therapeutically effective 30 dose" is defined as an amount sufficient to cure or at least partially arrest the disease and its complications in a patient already suffering from the disease. Amounts effective for WO 2009/155724 PCT/CH2009/000220 15 this use will depend upon the severity of the disorder being treated and the general state of the patient's own immune system. The term "subject" refers to any human or non-human animal. For example, the methods and compositions of the present invention can be used to treat a subject with a 5 VEGF-mediated disorder. The term "Min-graft" or "min" as used herein refers to a humanized variable domain that was generated by grafting of rabbit CDRs from a rabbit variable domain into a naturally occurring human acceptor framework (FW 1.4, SEQ ID No. 172). No changes in the framework regions are made. The framework itself was preselected for desirable 10 functional properties (solubility and stability). The term "Max-graft" or "max" as used herein refers to a humanized variable domain that was generated by grafting of rabbit CDRs from a rabbitvariable domain into the "rabbitized", human acceptor framework "RabTor" (rFWl.4, SEQ ID No. 173), or into a derivative thereof referred to as rFW 1.4(v2) (SEQ ID No. 174). The "RabTor" 15 framework was prepared by incorporating conserved rabbit residues (otherwisewhich are rather variable in other species) at framework positions generally involved in rabbit variable domain structure and stability, with the aim to generate a universally applicable framework that accepts virtually any set of rabbit CDRs without the need to graft donor framework residues other than at positions that are different in their presumable 20 progenitor sequence, e.g. that were altered during somatic hypermutation and thus, possibly contribute to antigen binding. The presumable progenitor sequence is defined to be the closest rabbit germline counterpart and in case the closest germline counterpart couldcan not be established, the rabbit subgroup consensus or the consensus of rabbit sequences with a high percentage of similarity. 25 The term "Min-Max" or "minmax" as used herein refers to a humanized variable domain comprising of a "Min-graft" variable light chain combined with a "Max-graft" variable heavy chain. The term "Max-Min" or "maxmin" as used herein refers to a humanized variable domain comprising of a "Max-graft" variable light chain combined with a "Min-graft" 30 variable heavy chain.

16 Different nomenclatures were used for the generated immunobinders. These are typically identified by a number (e.g. #578). In those cases where a prefix such as EP or Epi was used (e.g. EP 578 which is identical to Epi 578), the same immunobinder is thereby indicated. Occasionally, an immunobinder received a second designation which is identified by the prefix "ESBA". For example ESBA903 designates the same immunobinder as 578minmax or EP578minmax or Epi578minmax. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting. Various aspects of the invention are described in further detail in the following subsections. It is understood that the various embodiments, preferences and ranges may be combined at will. Further, depending on the specific embodiment, selected definitions, embodiments or ranges may not apply. Where the terms "comprise", "comprises", "comprised" or "comprising" are used in this specification (including the claims) they are to be interpreted as specifying the presence of the stated features, integers, steps or components, but not precluding the presence of one or more other features, integers, steps or components, or group thereof. The discussion of documents, acts, materials, devices, articles and the like is included in this specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all of these matters formed part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application. And- VEGF Immunobinders In one aspect, the present invention provides immunobinders that bind VEGF and thus are suitable to block the function of VEGF in vivo. The CDRs of these immunobinders are 16a derived from rabbit anti-VEGF monoclonal antibodies which were obtained from rabbits that were immunized with human VEGF and/or a fragment thereof (SEQ ID No.1). To our knowledge, this is the first time that monoclonal anti-VEGF antibodies were obtained from rabbits and characterized in detail. Surprisingly, the affinities (Kd) were found to be extraordinarily high. In certain embodiments, the invention provides an immunobinder, which specifically binds VEGF, comprising at least one of a CDRH 1, a CDRH2, a CDRH3, a CDRL1, a CDRL2, or a CDRL3 amino acid sequence. Exemplary CDR amino acid sequences for use in the immunobinders of the invention are set forth in SEQ ID Nos: 272 (Tables 1-6).

WO 2009/155724 PCT/CH2009/000220 17 Table 1. CDR HI amino acid sequences of anti-VEGF immunobinders of the invention. Sequence Identifier CDR-H1 SEQ ID No. 60-11-4 GFPFSSGYWVC 2 3 3 60-11-6 GFSFSSGYWIC 435 GFSLNTNYWMC 453 GFSFSRSYYIY 5 375 GFSFTTTDYMC 6 610 GIDFSGAYYMG 578 GFSLTDYYYMT 8 534 .GFSLSYYYMS 567 GFSLSDYYMC 10 509 GFSLSSYYMC 511 GFSLNTYYMN 12 13 509maxil GFSLSSYYMS ---------~~~~~~~~-- --- -- -- ----- --- --------------------- Consensus GFSLSSGYYMC 14 Table 2. CDR H2 amino acid sequences of antiVEGF immunobinders of the invention. ------------- --- ------ - - _ _ __ ---------- ------------ Sequence Identifier CDR-H2 SEQ ID No. 60 CIYAGSSGSTYYASWAKG 15 435 CMYTGSYNRAYYASWAKG 16 453 CIDAGSSGILVYANWAKG 375 CILAGDGSTYYANWAKG 18 610 YIDYDGDRYYASWAKG 578 FIDPDDDPYYATWAKG 20 534 IIGPGDYTDYASWAKG 21 57LYSDS K22 567 CLDYFGSTDDASWAKG WO 20091155724 PCTICH2009/000220 18 ------- ---- - ------------- __ ------------------------- 1T ------- YAWAK 24 59ai CLDYVGDTDYASWAKG 2 C511su CIDAGDDTYYASWAKG 2 ------

-

-----_ __ --------- 0al GNNYYIYTDGYAWAGF 27 375esu CIASDGDASSWSA 3 -61 ------------- -2------ Cosesu t GNDSSGYTDGGYAYWGIL, 2 435 QASNSSLA 453 QSSQSYVNNNRLA 29 WO 20091155724 PCT1CH2009/000220 19 375______ QASENINIWLS j4 610 QASQSISSWLS 142 578 QASEIIHSWLA 534 1QASQSIN1WLS 567 QADQSIYIWLS '509 QASQNIRIWLS 46 Sl11.a QASQSINIW CS -_-----------_---------___ Consensus i QASQSININNWL.S ________________ -_ ----------------------- ------------------------------ _____________________ TableS5. CDR L2 amino acid sequences of anti -VEGF imrnunobinders of tile invention. Sequence Identifier CDR-L2 SEQ ID No, 60 KASTLAS 5 - ------------- - ------ - --- -------------- 435 TAANI.AS 5 453 YASTLAS 5 375 QASKLAS 53 610 QASTLAS 5 578 LASTLAS 5 534 KESTLAS 56 567 KASTLES 509 KASTLES 5 511 RASTLAS Consensus KASTLAS 5 WO 2009/155724 PCT/CH2009/000220 20 Table 6. CDR L3 amino acid sequences of anti-VEGF immunobinders of the invention. Sequence Identifier CDR-L3 SEQ ID No. 60 QSNYGGSSSDYGNP 6 435 QNFATSDTVT 62 453 AGGYSSTSDNT 63 375 QNNYSYNRYGAP 64 610 QNNYGFRSYGGA 65 578 QNVYLASTNGAN 534 QNNYDSGNNGFP --------------- ------------- 567. QNNAHYSTNGGT 8 509 QNNAHYSTNGGT 69 511 QANYAYSAGYGAA Consensus QNNYHYSSSTNGGT 7 In one embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% 5 identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a consensus sequence of the group consisting of SEQ ID NO: 14, SEQ ID NO: 26, SEQ ID NO: 37, SEQ ID NO: 49, SEQ ID NO: 60 and SEQ ID NO: 71. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 14, SEQ ID NO: 26 and SEQ I) NO: 37 and/or the CDRs of the VL of said 10 immunobinder comprise CDRs of the group consisting of SEQ ID NO: 49, SEQ ID NO: 60 and SEQ ID NO: 71. Preferably, the CDR is selected from the group consisting of SEQ ID NO: 2 to SEQ ID NO: 13, SEQ ID NO: 15 to SEQ ID NO: 25, SEQ ID NO: 27 to SEQ ID NO: 36, SEQ ID NO: 38 to SEQ ID NO: 48, SEQ ID NO: 50 to SEQ ID NO: 59 and SEQ ID NO: 61 to SEQ ID NO: 70. 15 In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a sequence of the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID WO 2009/155724 PCT/CH2009/000220 21 NO: 15, SEQ ID NO: 27, SEQ ID NO: 38, SEQ ID NO: 50 and SEQ ID NO: 61. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 2, SEQ ID NO: 15 and SEQ ID NO: 27 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 38, SEQ ID NO: 5 50 and SEQ ID NO: 61. In another preferred embodiment, the VI-I of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 3, SEQ ID NO: 15 and SEQ I) NO: 27 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 38, SEQ ID NO: 50 and SEQ ID NO: 61. In another embodiment, the invention provides an immunobinder 10 comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a sequence of the group consisting of SEQ ID NO: 4, SEQ ID NO: 16, SEQ ID NO: 28, SEQ ID NO: 39, SEQ ID NO: 51, and SEQ ID NO: 62. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 4, SEQ 15 ID NO: 16, SEQ ID NO: 28 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 39, SEQ ID NO: 51, and SEQ ID NO: 62. In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% 20 identity to a sequence of the group consisting of SEQ ID NO: 5, SEQ ID NO: 17, SEQ ID NO: 29, SEQ ID NO: 40, SEQ ID NO: 52 and SEQ ID NO: 63. Preferably, the VH of said imrnmunobinder comprise the CDRs of the group consisting of SEQ ID NO: 5, SEQ ID NO: 17, SEQ ID NO: 29 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 40, SEQ ID NO: 52 and SEQ ID NO: 63. 25 In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a sequence of the group consisting of SEQ ID NO: 6, SEQ ID NO: 18, SEQ ID NO: 30, SEQ ID NO: 41, SEQ ID NO: 53 and SEQ ID NO: 64. Preferably, the VH of 30 said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 6, SEQ ID NO: 18 and SEQ ID NO: 30 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 41, SEQ ID NO: 53 and SEQ ID NO: 64. In another embodiment, the invention provides an immunobinder 35 comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% WO 2009/155724 PCT/CH2009/000220 22 identity to a sequence of the group consisting of SEQ ID NO: 7, SEQ ID NO: 19, SEQ ID NO: 31, SEQ ID NO: 42, SEQ ID NO: 54 and SEQ ID NO: 65. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 7, SEQ ID NO: 19 and SEQ ID NO: 31 and/or the CDRs of the VL of said immunobinder 5 comprise CDRs of the group consisting of SEQ ID NO: 42, SEQ ID NO: 54 and SEQ ID NO: 65. In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% 10 identity to a sequence of the group consisting of SEQ ID NO: 8, SEQ ID NO: 20; SEQ ID NO: 32, SEQ ID NO: 43, SEQ ID NO: 55 and SEQ I D NO: 66. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 8, SEQ ID NO: 20 and SEQ ID NO: 32 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 43, SEQ ID NO: 55 and SEQ ID 15 NO: 66. In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a sequence of the group consisting of SEQ ID NO: 9, SEQ ID NO: 21, SEQ ID 20 NO: 33, SEQ ID NO: 44, SEQ ID NO: 56 and SEQ ID NO: 67. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 9, SEQ ID NO: 21 and SEQ ID NO: 33 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 44, SEQ ID NO: 56 and SEQ ID NO: 67. 25 In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a sequence of the group consisting of SEQ ID NO: 10, SEQ ID NO: 22, SEQ ID NO: 34, SEQ ID NO: 45, SEQ ID NO: 57 and SEQ ID NO: 68. Preferably, the VH of 30 said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 10, SEQ ID NO: 22 and SEQ iD NO: 34 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 45, SEQ ID NO: 57 and SEQ ID NO: 68 In another embodiment, the invention provides an immunobinder 35 comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% WO 2009/155724 PCT/CH2009/000220 23 identity to a sequence of the group consisting of SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 35, SEQ ID NO: 46, SEQ ID NO: 58 and SEQ ID NO: 69. Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 11, SEQ ID NO: 23 and SEQ ID NO: 35 and/or the CDRs of 5 the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 46, SEQ ID NO: 58 and SEQ ID NO: 69. Alternatively, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 13, SEQ ID NO: 25 and SEQ ID NO: 35 and/or the CDRs of the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 46, SEQ ID NO: 58 and SEQ ID NO: 69. 10 In another embodiment, the invention provides an immunobinder comprising at least one CDR having at least 75% similarity, preferably at least 75% identity, more preferably at least 80%, 85%, 90% 95%, even more preferably 100% identity to a sequence of the group consisting of SEQ ID NO: 12, SEQ ID NO: 24, SEQ ID NO: 36, SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 59, and SEQ ID NO: 70. 15 Preferably, the VH of said immunobinder comprise the CDRs of the group consisting of SEQ ID NO: 12, SEQ ID NO: 24 and SEQ ID NO: 36. Additionally or alternatively, the VL of said immunobinder comprise CDRs of the group consisting of SEQ ID NO: 47, SEQ ID NO: 48, SEQ ID NO: 59, and SEQ ID NO: 70, e.g. SEQ ID NO: 47, SEQ ID NO: 59, and SEQ ID NO: 70; or SEQ ID NO: 48, SEQ ID NO: 59, and SEQ ID NO: 70. 20 In a much preferred embodiment, the immunobinder disclosed herein neutralizes human VEGF and is cross-reactive with rat/mouse VEGF or a portion thereof. The immunobinder can comprise an antibody or any alternative binding scaffold capable of accommodating CDRs. The CDRs set forth in SEQ ID Nos: 2-72 can be 25 grafted onto any suitable binding scaffold using any art recognized methods (see, e.g., Riechmann, L. el al. (1998) Nature 332:323-327; Jones, P. et al. (1986) Nature 321:522 525; Queen, C. et al. (1989) Proc. Nat. Acad. See. US.A. 86:10029-10033; U.S. Patent No. 5,225,539 to Winter, and U.S. Patent Nos. 5,530,101; 5,585,089; 5,693,762 and 6,180,370 to Queen et al.). However, it is preferred that the immunobinders disclosed 30 herein are humanized, and thus suitable for therapeutic applications. In the case of antibodies, the rabbit CDRs set forth in SEQ ID Nos: 2-72 may be grafted into the framework regions of any antibody from any species. However, it has previously been discovered that antibodies or antibody derivatives comprising the frameworks identified in the so called "quality control" screen (WOO148017) are WO 2009/155724 PCT/CH2009/000220 24 characterised by a generally high stability and/or solubility and thus may also be useful in the context of extracellular applications such as neutralizing human VEGF. Moreover, it has further been discovered that one particular combination of these VL (variable light chain) and VH (variable heavy chain) soluble and stable frameworks is particularly suited 5 to accommodating rabbit CDRs. Accordingly, in one embodiment, the CDRs set forth in SEQ ID Nos: 2-72 are grafted into the human antibody frameworks derived by "quality control" screening disclosed in EP1479694. The amino acid sequences of exemplary frameworks for use in the invention are set forth in SEQ ID Nos: 172 to 174. It was surprisingly found that upon grafting into said framework or its derivatives, loop 10 conformation of a large variety of rabbit CDRs could be fully maintained, largely independent of the sequence of the donor framework. Moreover, said framework or its derivatves containing different rabbit CDRs are well expressed and produced contrary to the rabbit wild type single chains and still almost fully retain the affinity of the original donor rabbit antibodies. 15 Thus, in a preferred embodiment, the CDRs and/or CDR motifs disclosed herein are present in a heavy chain variable region framework sequence having at least 80% sequence identity, more preferably at least 85%, 90% 95%, even more preferably 100% identity to the sequence of SEQ ID NO: 169. In a preferred embodiment, the heavy chain variable region framework sequence comprises SEQ ID NO: 170 or SEQ ID NO: 171. 20 In a preferred embodiment, the CDRs and/or CDR motifs disclosed herein are present in a light chain variable region framework sequence having at least 85% sequence identity, more preferably at least 90%, 95%, even more preferably 100% identity to the sequence of SEQ ID NO: 167, more preferably comprising SEQ ID NO: 167 or SEQ ID NO: 168. 25 In rabbit antibodies, CDRs can contain cysteine residues that become disulphide linked to cysteine residues in the antibody framework. Accordingly, it may be necessary, when grafting rabbit CDRs containing cysteine residues into non-rabbit framework regions to introduce cysteine residues in the non-rabbit framework by, for example, mutagenesis to facilitate the stabilization of rabbit CDR through a disulphide linkage. 30 In other embodiments, the invention provides an immunobinder, which specifically binds VEGF, comprising at least one of a VL or a VH amino acid sequence.

WO 2009/155724 PCT/CH2009/000220 25 Exemplary VH or VL amino acid sequences for use in the immunobinders of the invention are set forth in SEQ ID Nos: 72-106 and 107-166, respectively. In a preferred embodiment, the invention provides an immunobinder comprising a 5 VH having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 118, SEQ ID NO: 119, SEQ ID NO: 130 and SEQ ID NO: 131 (VH 60-11-4, VH 60-11-6, V-I 60-11-4min, VH 60-11-6min, VII 60-11-4max and VH 60-11-6max, respectively); 10 and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 72, SEQ ID NO:82 and SEQ ID NO: 93 (VL 60, VL 60min, VL 60max, respectively). 15 In another preferred embodiment, the invention provides an immunobinder comprising a V-I having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 109, SEQ ID NO: 120 and SEQ ID NO: 132 (VH 435, VH 435min and VH 435max, respectively); 20 and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 73, SEQ ID NO: 83 and SEQ ID NO:94 (VL 435, VL 435min and VL 435max, respectively). Preferably, said immunobinder has at least 80%, more preferably at least 85%, 25 90%, 95%, most preferably 100% identity to SEQ ID NO: 175 (435max). In another preferred embodiment, the invention provides an immunobinder comprising a VH1 having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID 30 NO: 110, SEQ ID NO: 121 and SEQ ID NO: 133 (VH 453, VH 453min and VH 453max, respectively); WO 2009/155724 PCT/CH2009/000220 26 and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ I) NO: 74, SEQ ID NO:84 and SEQ ID NO: 95(VL 453, VL 453min and VL 453max, respectively). 5 In another preferred embodiment, the invention provides an immunobinder comprising a VH having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: I 11, SEQ ID NO: 122 and SEQ ID NO: 134 (VH 375, VH 375min and VH 375max, 10 respectively); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 75, SEQ ID NO: 85 and SEQ ID NO:96 (VL 375, VL 375min and VL 375max, respectively). 15 In another preferred embodiment, the invention provides an immunobinder comprising a VH having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 112, SEQ ID NO: 123 and 135 (VII 610, VH 610min and VH 61Omax, 20 respectively); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 76, SEQ ID NO: 86 and SEQ ID NO: 97 (VL 610, VL 610min and VL 61Omax, respectively). 25 In another preferred embodiment, the invention provides an immunobinder comprising a VII having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 113, SEQ ID NO: 124, SEQ ID NO: 129, SEQ ID NO: 136, SEQ ID NO: 142, SEQ 30 ID NO: 144, SEQ ID NO: 146, SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID WO 2009/155724 PCT/CH2009/000220 27 NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:157, SEQ ID NO: 158, SEQ ID NO: 159, SEQ ID NO: 160, SEQ ID NO: 161, SEQ ID NO: 162, SEQ ID NO: 163, SEQ ID NO: 164, SEQ ID NO: 165 and SEQ ID NO: 166 (VII 578 and variants thereof); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most 5 preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 77, SEQ ID NO: 87, SEQ ID NO: 92, SEQ ID NO: 98, SEQ ID NO: 103, SEQ ID NO: 104 and SEQ ID NO: 105 (VL 578 and variants thereof). Preferably, said immunobinder has at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to SEQ ID NO: 178 (578min), SEQ ID NO: 10 179 (578max) or SEQ ID NO: 180 (578minmax). In another preferred embodiment, the inv&ntion-provides an immunobinder comprising a VHi having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID 15 NO: 114, SEQ ID NO: 125 and SEQ ID NO: 137 (VH 534, VH 534min and VH 534max, respectively); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 78, SEQ ID NO: 88 and SEQ ID NO: 99 (VL 534, VL 534min and VL 534max, 20 respectively). In another preferred embodiment, the invention provides an immunobinder comprising a VH having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID 25 NO: 115, SEQ ID NO: 126, SEQ ID NO:138 and SEQ ID NO: 143 (Vii 567, VH 567min and Vii 567max, respectively); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to-a sequence selected from the group consisting of SEQ 11) NO:. 79, SEQ ID NO:89 and SEQ ID NO: 100 (VL 567, VL 567min and VL 567max, 30 respectively).

WO 2009/155724 PCT/CH2009/000220 28 Preferably, said immunobinder has at least 80%, more preferably at least 85%, 90%, 95%. most preferably 100% identity to SEQ ID NO: 177 (567min). In another preferred embodiment, the invention provides an immunobinder 5 comprising a V-I having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 116, SEQ ID NO: 127. SEQ ID NO:139 and SEQ ID NO: 140 (VH 509, VH 509min, VII 509max and VH 509maxII, respectively); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most 10 preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 80, SEQ I) NO: 90 and SEQ ID NO: 101 (VL 509, VL 509min and VL 509max, respegtively). In another preferred embodiment, the invention provides an immunobinder 15 comprising a VH having at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 117, SEQ ID NO: 128, SEQ ID NO: 141 and SEQ ID NO: 145 (VH 511, VH 51 1min, VH 51 ]max and VH 511 maxDHP, respectively); and/or a VL having at least 80%, more preferably at least 85%, 90%, 95%, most 20 preferably 100% identity to a sequence selected from the group consisting of SEQ ID NO: 81, SEQ ID NO: 91, SEQ ID NO: 102 and SEQ ID NO: 106 (VL 511, VL 511min, VL 511max and VL 51 IminC41L, respectively). Preferably, said immunobinder has at least 80%, more preferably at least 85%, 90%, 95%, most preferably 100% identity to SEQ ID NO: 176 (51 Imax). 25 In certain embodiments, the invention further provides an immunobinder, which specifically binds VEGF, comprising an amino acid sequence with substantial similarity to an amino acid sequence set forth in SEQ ID Nos: 2-166 and in SEQ ID Nos: 175-180, and wherein the immunobinder essentially retains or improves the desired functional 30 properties of the anti-VEGF immunobinder of the invention. Preferred percentage WO 2009/155724 PCT/CH2009/000220 29 similarities include, but are not limited to, at least 50%, 60%, 70%, 75%, 80%, 85%, 90% or 95% identity. In certain embodiments, the invention further provides an immunobinder, which specifically binds VEGF, comprising an amino acid sequence with substantial identity to 5 an amino acid sequence set forth in SEQ ID Nos: 2-166 and in SEQ ID Nos. 175-180, and wherein the immunobinder retains or improves the desired functional properties of the anti-VEGF immunobinder of the invention. Preferred percentage identities include, but are not limited to, at least 50%, 60%, 70%, 75%, 80%, 85%, 90% or 95% identity. In certain embodiments, the invention further provides an immunobinder, which 10 specifically binds VEGF, comprising an amino acid sequence with conservative substitutions relative to an amino acid sequence set forth in SEQ ID Nos: 2-166 and in SEQ ID Nos. 475480, and wherein the immunobinder retains or improves-the-desired functional properties of the anti-VEGF immunobinder of the invention. In some embodiments, the invention provides immunobinders that bind 15 specifically to human VEGF and cross react with VEGF molecules of other species, for example, mouse VEGF, rat VEGF, rabbit VEGF or guinea pig VEGF. In a particular embodiment the anti-VEGF immunobinder can bind specifically to human and rat/mouse VEGF. In some embodiments, the invention provides immunobinders that bind 20 specifically to human VEGF and do not cross react with VEGF molecules of other species, for example, mouse VEGF, rat VEGF, rabbit VEGF or guinea pig VEGF. In some embodiments, the invention provides immunobinders that bind specifically to human VEGF and wherein the immunobinders are affinity matured. 25 In one embodiment, antibodies and antibody fragments of the present invention are single chain antibodies (scFv) or Fab fragments. In the case of scFv antibodies, a selected VL domain can be linked to a selected VH domain in either orientation by a - flexible linker. A suitable state of the art linker consists of repeated GGGGS amino acid sequences or variants thereof. In a preferred embodiment of the present invention a 30 (GGGGS) 4 linker of the amino acid sequence set forth in SEQ ID NO: 181, but variants of 1-3 repeats are also possible (Holliger et aL (1993), Proc. Nat. Acad. Sci. USA WO 2009/155724 PCT/CH2009/000220 30 90:6444-6448). Other linkers that can be used for the present invention are described by Alfthan et al. (1995), Protein Eng. 8:725-731, Choi et al. (200 1). Eur. J. Immunol. 31:94 106, Hu et al. (1996), Cancer Res. 56:3055-3061, Kipriyanov et al. (1999), J. Mol. Biol. 293:41-56 and Roovers et al. (200 1), Cancer Immunol. Immunother. 50:51-59. The 5 arrangement can be either VL-linker-VH or VH-linker-VL, with the former orientation being the preferred one. However, single VH or VL domain antibodies are also contemplated. In the case of Fab fragments, selected light chain variable domains VL are fused to the constant region of a human Ig kappa chain, while the suitable heavy chain variable domains VH are fused to the first (N-terminal) constant domain CHI of a human 10 IgG. At the C-terminus of the constant domain or at other sites of the variable or constant domain, an inter-chain disulfide bridge may be formed. Alternatively, the two chains may also be linked by a flexible linker resulting in a single chain Fab antibody. b. The antibodies or antibody derivatives of the present invention can have affinities to human VEGF with dissociation constants Kd in a range of 10- 4 M to 10~ 5 M. In a 15 preferred embodiment of the present invention the Kd is <1 nM. The affinity of an antibody for an antigen can be determined experimentally using a suitable method (Berzofsky et al. "Antibody-Antigen Interactions", in Fundamental Immunology, Paul, W.E., Ed, Raven Press: New York, NY (1992); Kuby, J. Immunology, W.H. Freeman and Company: New York, NY ) and methods described therein. 20 The company Epitomics sells an anti-VEGF antibody which is a rabbit monoclonal antibody (VEGF (C-term) Rabbit Antibody, Cat.no. 1909-1). Said antibody is directed against residues on the C-terminus of human VEGF and therefore not able to neutralize VEGF. Hence, said antibody is not suitable for therapeutic applications. Moreover, said monoclonal IgG is not a humanized antibody but is a natural rabbit full 25 length immunoglobulin. In addition, it was shown that this antibody does not recognize the native form of VEGF. Immunobinders that bind the same epitopes on VEGF In another aspect, the invention provides antibodies that bind to an epitope on 30 VEGF recognized by an antibody comprising any one of the amino acid sequences set forth in SEQ ID No 2-211. Such antibodies can be identified based upon their ability to WO 2009/155724 PCT/CH2009/000220 31 cross-compete with an antibody comprising any one or more of the amino acid sequences set forth in SEQ ID No 2-211 in standard VEGF-binding assays including, but not limited, to ELISA. The ability of a test antibody to inhibit the binding to human VEGF of an antibody comprising any one or more of the amino acid sequences set forth in SEQ 5 ID No 2-211 demonstrates that the test antibody can cross-compete thus interact with an overlapping epitope on human VEGF as an antibody comprising any one or more of the amino acid sequences set forth in SEQ ID No 2-211. Additionaly or alternatively, such antibodies can be also identified using standard epitope mapping techniques to determine if they bind to the same peptide immunogens. 10 Structural modelling techniques may also be employed to further define the precise molecular determinants to the antibody/VEGF interaction, including, but not limited to, NMR, X-ray crystallography, computer -based modeling, or protein tomography (Banyay et al., 2004 ASSAY and Drug Development Technologies (2), 5, Page 516-567). Indeed, the crystal structure of VEGF has been solved and the surface amino acid 15 residues involved in VEGFr binding are known (Fuh, et aL, 2006, . Biol. Chem., 281, 6625-6631). Accordingly, given the amino acid sequence of the peptide immunogen and the structural knowledge of VEGF available in the art, it is well within the skill in art to identify antibodies that bind to an epitope on VEGF recognized by the antibodies comprising any one or more of the amino acid sequences set forth in SEQ ID No 2-21 1. 20 In some embodiments, antibodies that bind to an epitope on VEGF recognized by an antibody comprising any one or more of the amino acid sequences set forth in SEQ ID No 2-211 bind to VEGF with an affinity of at least 10' M-', for example, at least 10" M', at least 108 M', at least 10 9 M", at least I0' MW , at least 10" M', at least 102 M- or at least 10" M. 25 In some embodiments, antibodies that bind to an epitope on VEGF recognized by an antibody comprising any one one or more of the amino acid sequences set forth in SEQ ID No 2-211 bind specifically to human VEGF and do not cross react with VEGF molecules of other species, for example, mouse VEGF, rat VGEF, rabbit VEGF, or guinea pig VEGF. 30 In some embodiments, antibodies that bind to an epitope on VEGF recognized by an antibody comprising any one or more of the amino acid sequences set forth in SEQ ID WO 2009/155724 PCT/CH2009/000220 32 No 2-2 I cross react with VEGF molecules of other species, for example, mouse VEGF, rat VEGF, or rabbit VEGF. Optimized Variants 5 The antibodies of the invention may be further optimized for enhanced functional properties, e.g., for enhanced solubility and/or stability. In certain embodiments, the antibodies of the invention are optimized according to the "functional consensus" methodology disclosed in PCT Application Serial No. PCT/EP2008/001958, entitled "Sequence Based Engineering and Optimization of Single 10 Chain Antibodies", filed on March 12, 2008, which is incorporated herein by reference. For example, the VEGF immunobinders of the invention can be compared with a database of functionally-selected scFvs to identify amino acid residue positions that are either more or less tolerant of variability than the corresponding position(s) in the VEGF immunobinder, thereby indicating that such identified residue position(s) may be suitable 15 for engineering to improve functionality such as stability and/or solubility. Exemplary framework positions for substitution are described in PCT Application No. PCT/CH2008/000285. entitled "Methods of Modifying Antibodies, and Modified Antibodies with Improved Functional Properties", filed on June 25, 2008, and PCT Application No. PCT/CH2008/000284, entitled "Sequence Based Engineering and 20 Optimization of Single Chain Antibodies", filed on June 25, 2008. For example, one or more of the following substitutions may be introduced at an amino acid position (AHo numbering is referenced for each of the amino acid position listed below) in the heavy chain variable region of an immunobinder of the invention: (a) Q or E at amino acid position 1; 25 (b) Q or E at amino acid position 6; (c) T, S or A at amino acid position 7, more preferably T or A, even more preferably T; (d) A, T, P, V or D, more preferably T, P, V or D, at amino acid position 10, 30 (e) L or V, more preferably L, at amino acid position 12, WO 2009/155724 PCT/CH2009/000220 33 (f V, R, Q, M or K. more preferably V, R, Q or M at amino acid position 13; (g) R, M, E, Q or K, more preferably R, M, E or Q, even more preferably R or E, at amino acid position 14; 5 (h) L or V, more preferably L, at amino acid position 19; (i) R, T, K or N, more preferably R, T or N, even more preferably N, at amino acid position 20; (j) 1, F, L or V, more preferably 1, F or L, even more preferably I or L, at amino acid position 21; 10 (k) R or K, more preferably K, at amino acid position 45; (1) T, P, V, A or R, more preferably T, P, V or R, even more preferably R, at-amino acid position 47; (m) K, Q, H or E, more preferably K, 1-1 or E, even more preferably K, at amino acid position 50; 15 (n) M or 1, more preferably I, at amino acid position 55; (o) K or R, more preferably K, at amino acid position 77; (p) A, V, L or 1, more preferably A, L or 1, even more preferably A, at amino acid position 78; (q) E, R, T or A, more preferably E, T or A, even more preferably E, at 20 amino acid position 82; (r) T, S, I or L, more preferably T, S or L, even more preferably T, at amino acid position 86; (s) D, S, N or (, more preferably D, N or G, even more preferably N, at amino acid position 87; 25 (t) A. V, L or F, more preferably A, V or F, even more preferably V, at amino acid position 89; (u) F, S, H, D or Y, more preferably F, S, H or D, at amino acid position 90; (v) D, Q or E, more preferably D or Q, even more preferably D, at amino 30 acid position 92; WO 2009/155724 PCT/CH2009/000220 34 (w) G, N, T or S, more preferably G, N or T, even more preferably G, at amino acid position 95; (x) T, A, P, F or S, more preferably T, A, P or F, even more preferably F, at amino acid position 98; 5 (y) R, Q, V, 1, M, F, or L, more preferably R, Q, 1, M, F or L, even more preferably Y, even more preferably L, at amino acid position 103; and (z) N, S or A, more preferably N or S, even more preferably N, at amino acid position 107. Additionally or alternatively, one or more of the following substitutions can be 10 introduced into the light chain variable region of an immunobinder of the invention: (aa) Q, D, L, E, S, or 1, more preferably L, E, S or 1, even more preferably L or E, at amino acid-position 1; (bb) S, A, Y, I, P or T, more preferably A, Y, I, P or T, even more preferably P or T at amino acid position 2; 15 (cc) Q, V, T or I, more preferably V, T or 1, even more preferably V or T, at amino acid position 3; (dd) V, L, I or M, more preferably V or L, at amino acid position 4; (ee) S, E or P, more preferably S or E, even more preferably S, at amino acid position 7; 20 (ff) T or I, more preferably I, at amino acid position 10; (gg) A or V, more preferably A, at amino acid position 11; (hh) S or Y, more preferably Y, at amino acid position 12; (ii) T, S or A, more preferably T or S, even more preferably T, at amino acid position 14; 25 (jj) S or R, more preferably S, at amino acid position 18; (kk) T or R, more preferably R, at amino acid position 20; (11) R or Q, more preferably Q, at amino acid position 24; (mm) H or Q, more preferably H, at amino acid position 46; (nn) K, R or I, more preferably R or 1, even more preferably R, at amino 30 acid position 47; WO 2009/155724 PCT/CH2009/000220 35 (oo) R, Q. K, E, T, or M, more preferably Q, K, E, T or M, at amino acid position 50; (pp) K, T, S, N, Q or P, more preferably T, S, N, Q or P, at amino acid position 53; 5 (qq) I or M, more preferably M, at amino acid position 56; (rr) H, S, F or Y, more preferably H, S or F, at amino acid position 57; (ss) 1, V or T, more preferably V or T, R, even more preferably T, at amino acid position 74; (tt) R, Q or K, more preferably R or Q, even more preferably R, at amino 1 acid position 82; (uu) L or F, more preferably F, at amino acid position 91; (vv) G, D, T or A, more preferably G, ) or T, even more-preferably T, at amino acid position 92; (xx) S or N, more preferably N, at amino acid position 94; 15 (yy) F, Y or S, more preferably Y or S, even more preferably S, at amino acid position 101; and (zz) D, F, -1, E, L, A, T, V, S, G or 1, more preferably H, E, L, A, T ,V, S, G or I, even more preferably A or V, at amino acid position 103. The AHo numbering system is described further in Honegger, A. and Pluckthun, 20 A. (200 1) J Mol. Biol. 309:657-670). Alternatively, the Kabat numbering system as described further in Kabat et al. (Kabat, E. A., et al. (1991) Sequences of Proteins of Immunological Interest, Fifth Edition, U.S. Department of Health and Human Services, NI-I Publication No. 91-3242) may be used. Conversion tables for the two different numbering systems used to identify amino acid residue positions in antibody heavy and 25 light chain variable regions are provided in A. Honegger, J.Mol.Biol. 309 (2001) 657 670. In other embodiments, the immunobinders of the invention comprise one or more of the solubility and/or stability enhancing mutations described in U.S. Provisional Application Serial No. 61/075,692, entitled "Solubility Optimization of Immunobinders," 30 filed on June 25, 2008. In certain preferred embodiments, the immunobinder comprises a solubility enhancing mutation at an amino acid position selected from the group of heavy WO 2009/155724 PCT/CH2009/000220 36 chain amino acid positions consisting of 12, 103 and 144 (AHo Numbering convention). In one preferred embodiment, the immunobinder comprises one or more substitutions selected from the group consisting of: (a) Serine (S) at heavy chain amino acid position 12; (b) Serine (S) or Threonine (T) at heavy chain amino acid position 103; and (c) Serine 5 (S) or Threonine (T) at heavy chain amino acid position 144. In another embodiment, the immunobinder comprises the following substitutions: (a) Serine (S) at heavy chain amino acid position 12; (b) Serine (S) or Threonine (T) at heavy chain amino acid position 103; and (c) Serine (S) or Threonine (T) at heavy chain amino acid position 144. 10 Hybridomas Expressing Rabbit Anti-VEGF Antibodies In another aspect, the invention provides a hybridoma expressing a monoclonal antibody comprising any one or more-of-the amino acid sequences set forth in SEQ ID Nos 72-81 and SEQ ID Nos 107-117. Methods for generating hybridomas from Rabbit B-cells are well known in the art and are disclosed, for example, in U.S. patent 15 application 2005/0033031. Production of Anti-VEGF Immunobinders The antibodies or antibody derivatives of the present invention may be generated using routine techniques in the field of recombinant genetics. Knowing the sequences of 20 the polypeptides, the cDNAs encoding them can be generated by gene synthesis (www.genscript.com). These cDNAs can be cloned into suitable vector plasmids. Once the DNA encoding a VL and/or a VH domain are obtained, site directed mutagenesis, for example by PCR using mutagenic primers, can be performed to obtain various derivatives. The best "starting" sequence can be chosen depending on the number of 25 alterations desired in the VL and/or VH sequences. Methods for incorporating or grafting CDRs into framework regions include those set forth in, e.g., Riechmann, L. et al. (1998) Aature 332:323-327; Jones, P. et al. (1986) Nature 321:522-525; Queen, C. et al. (1989) Proc. Natl. A cad. See. US.A. 86:10029 10033; U.S. Patent No. 5,225,539 to Winter, and U.S. Patent Nos. 5,530,101; 5,585,089; 30 5,693,762 and 6,180,370 to Queen et al, as well as those disclosed in U.S. Provisional WO 2009/155724 PCT/CH2009/000220 37 Application Serial No. 61/075,697, entitled "Humanization of Rabbit Antibodies Using Universal Antibody Frameworks," filed on June 25, 2008. Standard cloning and mutagenesis techniques well known to the person skilled in the art can be used to attach linkers, shuffle domains or construct fusions for the 5 production of Fab fragments. Basic protocols disclosing the general methods of this invention are described in Molecular Cloning, A Laboratory Manual (Sambrook & Russell, 3'd ed. 2001) and in Current Protocols in Molecular Biology (Ausubel et al., 1999). The DNA sequence harboring a gene encoding a scFv polypeptide, or in the case 10 of Fab fragments, encoding either two separate genes or a bi-cistronic operon comprising the two genes for the VL-CK and the VH-CHI fusions are cloned in a suitable expression vector, preferably one with-aninducible promoter. Care must be taken that in front of each gene an appropriate ribosome binding site is present that ensures translation. It is to be understood that the antibodies of the present invention comprise the disclosed 15 sequences rather than they consist of them. For example, cloning strategies may require that a construct is made from which an antibody with one or a few additional residues at the N-terminal end are present. Specifically, the methionine derived from the start codon may be present in the final protein in cases where it has not been cleaved posttranslationally. Most of the constructs for scFv antibodies give rise to an additional 20 alanine at the N-terminal end. In a preferred embodiment of the present invention, an expression vector for periplasmic expression in E. coli is chosen (Krebber, 1997). Said vector comprises a promoter in front of a cleavable signal sequence. The coding sequence for the antibody peptide is then fused in frame to the cleavable signal sequence. This allows the targeting of the expressed polypeptide to the bacterial periplasm where the 25 signal sequence is cleaved. The antibody is then folded. In the case of the Fab fragments, both the VL-CK and the V-I-CHI fusions peptides must be linked to an export signal. The covalent S-S bond is formed at the C-terminal cysteines after the peptides have reached the periplasm. If cytoplasmic expression of antibodies is preferred, said antibodies usually can be obtained at high yields from inclusion bodies, which can be easily 30 separated from other cellular fragments and protein. In this case the inclusion bodies are WO 2009/155724 PCT/CH2009/000220 38 solubilized in a denaturing agent such as, e.g. guanidine hydrochloride (GndHCl) and then refolded by renaturation procedures well known to those skilled in the art. Plasmids expressing the scFv or Fab polypeptides are introduced into a suitable host, preferably a bacterial, yeast or mammalian cell, most preferably a suitable E. coli 5 strain as for example JM83 for periplasmic expression or BL21 for expression in inclusion bodies. The polypeptide can be harvested either from the periplasm or form inclusion bodies and purified using standard techniques such as ion exchange chromatography, reversed phase chromatography, affinity chromatography and/or gel filtration known to the person skilled in the art. 10 The antibodies or antibody derivatives of the present invention can be characterized with respect to yield, solubility and stability in vitro. Binding capacities towards VEGF, preferably towards humanVEGF, can be tested in vitro by ELISA or surface plasmon resonance (BIACore), using recombinant human VEGF as described in W09729131, the latter method also allowing to determine the kg. rate constant, which 15 should preferably be less than 1 0 . Kd values of 10 nM are preferred. Aside from antibodies with strong binding affinity for human VEGF, it is also desirable to select anti-VEGF antibodies which have other beneficial properties from a therapeutic perspective. For example, the antibody may be one which inhibits HUVEC cell growth in response to VEGF (see Example 3). In one embodiment, the antibody may 20 be able to inhibit HUVEC cell proliferation in response to a near maximally effective concentration of VEGF (0.08 nM). Preferably, the antibody has an effective dose 50 (ED50) value of no more than about 5 nM, preferably no more than about I nM, preferably no more than about I nM, preferably no more than about 0.5 nM and most preferably no more than about 0.06 nM, for inhibiting VEGF-induced proliferation of 25 endothelial cells in this "endothelial cell growth assay", i.e., at these concentrations the antibody is able to inhibit VEGF-induced endothelial cell growth in vitro by, e.g., 50% or more. Bispecific Molecules 30 In another aspect, the present invention features bispecific molecules comprising an anti-VEGF antibody, or a fragment thereof, of the invention. An antibody of the WO 2009/155724 PCT/CH2009/000220 39 invention, or antigen-binding portions thereof, can be derivatized or linked to another functional molecule, e.g., another peptide or protein (e.g., another antibody or ligand for a receptor) to generate a bispecific molecule that binds to at least two different binding sites or target molecules. The antibody of the invention may be derivatized or linked to 5 more than one other functional molecule to generate multispecific molecules that bind to more than two different binding sites and/or target molecules; such multispecific molecules are also intended to be encompassed by the term "bispecific molecule" as used herein. To create a bispecific molecule of the invention, an antibody of the invention can be functionally linked (e.g., by chemical coupling, genetic fusion, noncovalent 10 association or otherwise) to one or more other binding molecules, such as another antibody, antibody fragment, tumor specific or pathogen specific antigens, peptide or binding mimetic, such that a bispecific molecule results. Accordingly, the present invention includes bispecific molecules comprising at least one first binding molecule having specificity for VEGF and a second binding molecule having specificity for one or 15 more additional target epitope. In one embodiment, the bispecific molecules of the invention comprise a binding specificity at least one antibody, or an antibody fragment thereof, including, e.g,, an Fab, Fab', F(ab') 2 , Fv, or a single chain Fv. The antibody may also be a light chain or heavy chain dimer, or any minimal fragment thereof such as a Fv or a single chain construct as 20 described in Ladner et al. U.S. Patent No. 4,946,778, the contents of which is expressly incorporated by reference. While human monoclonal antibodies are preferred, other antibodies which can be employed in the bispecific molecules of the invention are murine, chimeric and humanized monoclonal antibodies. 25 The bispecific molecules of the present invention can be prepared by conjugating the constituent binding specificities using methods known in the art. For example, each binding specificity of the bispecific molecule can be generated separately and then conjugated to one another. When the binding specificities are proteins or peptides, a variety of coupling or cross-linking agents can be used for covalent conjugation. 30 Examples of cross-linking agents include protein A, carbodiimide, N-succinimidy-S acetyl-thioacetate (SATA), 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB), o- WO 2009/155724 PCT/CH2009/000220 40 phenylenedirnaleimide (oPDM), N-succinimidyl-3-(2-pyridyldith io)propionate (SPDP), and sulfosuccinirnidyl 4-(N-maleimidomethyl) cyclohaxane-1-carboxylate (sulfo-SMCC) (see e.g, Karpovsky et al. (1984) J. Exp. Med. 160:1686; Liu, MA et at (1985) Proc. Natl. A cad Sci. USA .82:8648). Other methods include those described in Paulus (1985) 5 Behring Ins. Mitt. No. 78, 118-132; Brennan et al. (1985) Science 229:81-83), and Glennie et al. (1987) J. Immunol. 139: 2367-2375). Preferred conjugating agents are SATA and sulfo-SMCC, both available from Pierce Chemical Co. (Rockford, IL). When the binding specificities are antibodies, they can be conjugated via sulfhydryl bonding, for example, via the C-terminus hinge regions of the two heavy 10 chains or other sites, whether naturally occurring or introduced artificially. In a particularly preferred embodiment, the hinge region is modified to contain an odd numbersof sulfhydryl residues, preferably one, prior to conjugation Alternatively, both binding specificities can be encoded in the same vector and expressed and assembled in the same host cell. This method is particularly useful 15 where the bispecific molecule is a mAb x mAb, mAb x Fab, Fab x F(ab') 2 or ligand x Fab fusion protein. A bispecific molecule of the invention can be a single chain molecule comprising one single chain antibody and a binding determinant, or a single chain bispecific molecule comprising two binding determinants. Bispecific molecules may comprise at least two single chain molecules. Further, a bispecfic molecule may be a 20 scFv that specifically binds to first target, wherein theVH and VL of said scFv are linked with a flexible linker comprising a domain providing specific binding to a second target. Suitable linkers are described in U.S. Provisional Application No. 60/937,820. Methods for preparing bispecific molecules are described for example in U.S. Patent Number 5,260,203; U.S. Patent Number 5,455,030; U.S. Patent Number 4,881,175; U.S. Patent 25 Number 5,132,405; U.S. Patent Number 5,091,513; U.S. Patent Number 5,476,786; U.S. Patent Number 5,013,653; U.S. Patent Number 5,258,498; and U.S. Patent Number 5,482,858. Binding of the bispecific molecules to their specific targets can be confirmed by, for example, enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), 30 FACS analysis, bioassay (e.g., growth inhibition), or by immunoblot assay. Each of these assays generally detects the presence of protein-antibody complexes of particular WO 2009/155724 PCT/CH2009/000220 41 interest by employing a labeled reagent (e.g., an antibody) specific for the complex of interest. For example, the VEGF-antibody complexes can be detected using e.g., an enzyme-linked antibody or antibody fragment which recognizes and specifically binds to the antibody-VEGF complexes. Alternatively, the complexes can be detected using any 5 of a variety of other immunoassays. For example, the antibody can be radioactively labeled and used in a radioimrmunoassay (RIA) (see, for example, Weintraub, B., Principles of Radioimmunoassays, Seventh Training Course on Radioligand Assay Techniques, The Endocrine Society, March, 1986, which is incorporated by reference herein). The radioactive isotope can be detected by such means as the use of a y counter 10 or a scintillation counter or by autoradiography. Immunoconjugafes In another aspect, the present invention features an anti-VEGF antibody, or a fragment thereof, conjugated to a therapeutic moiety, such as a cytotoxin, a drug (e.g., an 15 immunosuppressant) or a radiotoxin. Such conjugates are referred to herein as "immunoconjugates". Immunoconjugates that include one or more cytotoxins are referred to as "immunotoxins." A cytotoxin or cytotoxic agent includes any agent that is detrimental to (e.g., kills) cells. Examples include taxol, cytochalasin B, gramicidin D, ethidium bromide, emetine, mitomycin, etoposide, tenoposide, vincristine, vinblastine, 20 colchicin, doxorubicin, daunorubicin, dihydroxy anthracin dione, mitoxantrone, mithramycin, actinomycin D, I -dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, and puromycin and analogs or homologs thereof. Therapeutic agents also include, for example, antimetabolites (e.g., methotrexate, 6 mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouracil decarbazine), alkylating agents 25 (e.g., mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU) and lomustine (CCNU), cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cis-dichlorodiamine platinum (II) (DDP) cisplatin), anthracyclines (e.g., daunorubicin (formerly daunomycin) and doxorubicin), antibiotics (e.g., dactinomycin (formerly actinomycin), bleomycin, mithramycin, and anthramycin 30 (AMC)), and anti-mitotic agents (e.g., vincristine and vinblastine).

WO 2009/155724 PCT/CH2009/000220 42 Other preferred examples of therapeutic cytotoxins that can be conjugated to an antibody of the invention include duocarmycins, calicheamicins, maytansines and auristatins, and derivatives thereof. An example of a calicheamicin antibody conjugate is commercially available (MylotargTM; Wyeth-Ayerst). 5 Cytotoxins can be conjugated to antibodies of the invention using linker technology available in the art. Examples of linker types that have been used to conjugate a cytotoxin to an antibody include, but are not limited to, hydrazones, thioethers, esters, disulfides and peptide-containing linkers. A linker can be chosen that is, for example, susceptible to cleavage by low p-I within the lysosomal compartment or 10 susceptible to cleavage by proteases, such as proteases preferentially expressed in tumor tissue such as cathepsins (e.g., cathepsins B, C, D). For further discussion oftypes-of cytotoxins, linkers and methods for conjugating therapeutic agents to antibodies, see also Saito, G. el al. (2003) Adv. Drug Deliv. Rev. 55:199-215; Trail, PA. et al. (2003) Cancer Immunol. Immunother. 52:328-337; Payne, 15 G. (2003) Cancer Cell 3:207-212; Allen, T.M. (2002) Nat. Rev. Cancer 2:750-763; Pastan, 1. and Kreitman, R. J. (2002) Curr. Opin. Investig. Drugs 3:1089-1091; Senter, P.D. and Springer, C.J. (2001) Adv. Drug Deliv. Rev. 53:247-264. Antibodies of the present invention also can be conjugated to a radioactive isotope to generate cytotoxic radiopharmaceuticals, also referred to as radioimmunoconjugates. 20 Examples of radioactive isotopes that can be conjugated to antibodies for being used diagnostically or therapeutically include, but are not limited to, iodine'3, indium ', yttrium and lutetium 7 . Methods for preparing radioimmunconjugates are established in the art. Examples of radioimmunoconjugates are commercially available, including ZevalinTM (IDEC Pharmaceuticals) and BexxarTM (Corixa Pharmaceuticals), and similar 25 methods can be used to prepare radioimmunoconjugates using the antibodies of the invention. The antibody conjugates of the invention can be used to modify a given biological response, and the drug moiety is not to be construed as limited to classical chemical therapeutic agents. For example, the drug moiety may be a protein or polypeptide 30 possessing a desired biological activity. Such proteins may include, for example, an enzymatically active toxin, or active fragment thereof, such as abrin, ricin A, WO 2009/155724 PCTiCH2009/000220 43 pseudomonas exotoxin, or diphtheria toxin; a protein such as tumor necrosis factor or interferon-y; or, biological response modifiers such as, for example, lymphokines, interleukin-1 ("IL-I"), interleukin-2 ("IL-2"), interleukin-6 ("IL-6"), granulocyte macrophage colony stimulating factor ("GM-CSF"), granulocyte colony stimulating 5 factor ("G-CSF"), or other growth factors. Techniques for conjugating such therapeutic moiety to antibodies are well known, see, e.g., Arnon et al., "Monoclonal Antibodies For Immunotargeting Of Drugs In Cancer Therapy", in MonoclonalAntibodies And Cancer Therapy, Reisfeld et al. (eds.), pp. 243 56 (Alan R. Liss, Inc. 1985); Hellstrom et al., "Antibodies For Drug Delivery", in 10 Controlled Drug Delivery (2nd Ed.), Robinson et al. (eds.), pp. 623-53 (Marcel Dekker, Inc. 1987); Thorpe, "Antibody Carriers Of Cytotoxic Agents In Cancer Therapy: A Review", in Monoclonal Antibodies '84: Biological And Clinical Applications, Pinchera et al. (eds.), pp. 475-506 (1985); "Analysis, Results, And Future Prospective Of The Therapeutic Use Of Radiolabeled Antibody In Cancer Therapy", in Monoclonal 1 5 Antibodies For Cancer Detection And Therapy, Baldwin et al. (eds.), pp. 303-16 (Academic Press 1985), and Thorpe et al., "The Preparation And Cytotoxic Properties Of Antibody-Toxin Conjugates", Immunol. Rev., 62:1 19-58 (1982). Ues ofAnti-VEGF anibodies 20 For therapeutic applications, the anti-VEGF antibodies of the invention are administered to a mammal, preferably a human, in a pharmaceutically acceptable dosage form such as those discussed herein, including those that may be administered to a human intravenously, as a bolus or by continuous infusion over a period of time, by topical, intraocular, intramuscular, intraperitoneal, intra-cerebrospinal, subcutaneous, intra 25 articular, intrasynovial, intrathecal, oral, or inhalation routes. The antibodies also are suitably administered by intra tumoral, peritumoral, intralesional, or perilesional routes, to exert local as well as systemic therapeutic effects. The intraperitoneal route is expected to be particularly useful, for example, in the treatment of ovarian tumors. For the prevention or treatment of disease, the appropriate dosage of antibody will 30 depend on the type of disease to be treated, as defined above, the severity and course of the disease, whether the antibody is administered for preventive or therapeutic purposes, WO 2009/155724 PCT/CH2009/000220 44 previous therapy, the patient's clinical history and response to the antibody, and the discretion of the attending physician. The antibody is suitably administered to the patient at one time or over a series of treatments. The anti-VEGF antibodies are useful in the treatment of VEGF-mediated diseases 5 as described herein. For example, age-related macular degeneration (AMD) is a leading cause of severe visual loss in the elderly population. The exudative form of AMD is characterized by choroidal neovascularization and retinal pigment epithelial cell detachment. Because choroidal neovascularization is associated with a dramatic worsening in prognosis, the VEGF antibodies of the present invention are especially 10 useful in reducing the severity of AMD. The progress of this therapy is easily monitored by conventional techniques including opthalmoscopy, ocular fundus microscopy, and ocular computer tomography. All FDA approved doses and regimes suitable for use with Lucentis are considered. Other doses and regimes are described in U.S. Provisional Application Serial 15 No. 61/075,641, entitled "Improved Immunobinder Formulations And Methods For Adminstration", filed June 25, 2008, and U.S. Provisional Application No. 61/058,504, which are expressly incorporated herein. According to another embodiment of the invention, the effectiveness of the antibody in preventing or treating disease may be improved by administering the 20 antibody serially or in combination with another agent that is effective for those purposes, such as tumor necrosis factor (TNF), an antibody capable of inhibiting or neutralizing the angiogenic activity of acidic or basic fibroblast growth factor (FGF) or hepatocyte growth factor (HGF), an antibody capable of inhibiting or neutralizing the coagulant activities of tissue factor, protein C, or protein S (see Esmon et al., PCT Patent Publication No. WO 25 91/01753, published 21 Feb, 1991), an antibody capable of binding to HER2 receptor (see Hudziak et al., PCT Patent Publication No. WO 89/06692, published 27 Jul. 1989), or one or more conventional therapeutic agents such as, for example, alkylating agents, photocoagulants (such as verteporfin), folic acid antagonists, anti-metabolites of nucleic acid metabolism, antibiotics, pyrimidine analogs, 5-fluorouracil, cisplatin, purine 30 nucleosides, amines, amino acids, triazol nucleosides, or corticosteroids. Such other agents may be present in the composition being administered or may be administered WO 2009/155724 PCT/CH2009/000220 45 separately. Also, the antibody is suitably administered serially or in combination with radiological treatments, whether involving irradiation or administration of radioactive substances. The antibodies of the invention may be used as affinity purification agents. In this 5 process, the antibodies are immobilized on a solid phase such a Sephadex resin or filter paper, using methods well known in the art. The immobilized antibody is contacted with a sample containing the VEGF protein (or fragment thereof) to be purified, and thereafter the support is washed with a suitable solvent that will remove substantially all the material in the sample except the VEGF protein, which is bound to the immobilized 10 antibody. Finally, the support is washed with another suitable solvent, such as glycine buffer, pH 5.0, that will release the VEGF protein from the antibody. Anti-VEGF antibodies may also be usefullin diagnostic assays for VEGF protein, e.g., detecting its expression in specific cells, tissues, or serum. Such diagnostic methods may be useful in cancer diagnosis. 15 For diagnostic applications, the antibody typically will be labeled with a detectable moiety. Numerous labels are available which can be generally grouped into the following categories: (a) Radioisotopes, such as '' In, 9 Tc, " C, 131 I 1251, 3 H, 3 2 P or 3 5 S. The antibody can be labeled with the radioisotope using the techniques described in Current 20 Protocols in Immunology, Volumes I and 2, Coligen et al., Ed. Wiley-Interscience, New York, N.Y., Pubs. (1991), for example, and radioactivity can be measured using scintillation counting. (b) Fluorescent labels such as rare earth chelates (europium chelates) or fluorescein and its derivatives, rhodamine and its derivatives, dansyl, Lissamine, 25 phycoerythrin and Texas Red are available. The fluorescent labels can be conjugated to the antibody using the techniques disclosed in Current Protocols in Immunology, supra, for example. Fluorescence can be quantified using a fluorimeter. (c) Various enzyme-substrate labels are available and U.S. Pat. No. 4,275,149 provides a review of some of these. The enzyme generally catalyzes a chemical alteration 30 of the chromogenic substrate which can be measured using various techniques. For example, the enzyme may catalyze a color change in a substrate, which can be measured WO 2009/155724 PCT/CH2009/000220 46 spectrophotometrically. Alternatively, the enzyme may alter the fluorescence or chemiluminescence of the substrate. Techniques for quantifying a change in fluorescence are described above. The chemiluminescent substrate becomes electronically excited by a chemical reaction and may then emit light which can be measured (using a 5 chemiluminometer, for example) or donates energy to a fluorescent acceptor. Examples of enzymatic labels include luciferases (e.g., firefly luciferase and bacterial luciferase; U.S. Pat. No. 4,737,456), luciferin, 2,3-dihydrophthalazinediones, malate dehydrogenase, urease, peroxidase such as horseradish peroxidase (HRPO), alkaline phosphatase, .beta.

galactosidase, glucoamylase, lysozyme, saccharide oxidases (e.g., glucose oxidase, 10 galactose oxidase, and glucose-6-phosphate dehydrogenase), heterocyclic oxidases (such as uricase and xanthine oxidase), lactoperoxidase, microperoxidase, and the like. Techniques for.conjugating enzymes to antibodies are described in O'Sullivanet aL, Methods for the Preparation of Enzyme-Antibody Conjugates for use in Enzyme Immunoassay, in Methods in Enzym. (ed J. Langone & H. Van Vunakis), Academic 15 press, New York, 73:147-166 (198 1). Examples of enzyme-substrate combinations include, for example: (i) Horseradish peroxidase (HRPO) with hydrogen peroxidase as a substrate, wherein the hydrogen peroxidase oxidizes a dye precursor (e.g., orthophenylene diamine (OPD) or 3,3',5,5'-tetramethyl benzidine hydrochloride (TMB)); 20 (ii) alkaline phosphatase (AP) with para-Nitrophenyl phosphate as chromogenic substrate; and (iii) .beta.-D-galactosidase (.beta.-D-Gal) with a chromogenic substrate (e.g., P nitrophenyl-.beta. -D-galactosidase) or fluorogenic substrate 4-methyl umbell iferyl-.beta.

D-galactosidase. 25 In another embodiment of the invention, the anti-VEGF antibody need not be labeled, and the presence thereof can be detected using a labeled antibody which binds to the VEGF antibody. The antibodies of the present invention may be employed in any known assay method, such as competitive binding assays, direct and indirect sandwich assays, and 30 immunoprecipitation assays. Zola, Monoclonal Antibodies: A Manual of Techniques, pp.147-158 (CRC Press, Inc. 1987).

WO 2009/155724 PCT/CH2009/000220 47 Competitive binding assays rely on the ability of a labeled standard to compete with the test sample analyte for binding with a limited amount of antibody. The amount of VEGF protein in the test sample is inversely proportional to the amount of standard that becomes bound to the antibodies. To facilitate determining the amount of standard 5 that becomes bound, the antibodies generally are insolubilized before or after the competition, so that the standard and analyte that are bound to the antibodies may conveniently be separated from the standard and analyte which remain unbound. Sandwich assays involve the use of two antibodies, each capable of binding to a different immunogenic portion, or epitope, of the protein to be detected. In a sandwich 10 assay, the test sample analyte is bound by a first antibody which is immobilized on a solid support, and thereafter a second antibody binds to the analyte, thus forming an insoluble three-part complex. See, e.g., U.S. Pat. No. 4,376;J1 0 The second antibody may itself be labeled with a detectable moiety (direct sandwich assays) or may be measured using an anti-immunoglobulin antibody that is labeled with a detectable moiety 15 (indirect sandwich assay). For example, one type of sandwich assay is an ELISA assay, in which case the detectable moiety is an enzyme. For immunohistochemistry, the tumor sample may be fresh or frozen or may be embedded in paraffin and fixed with a preservative such as formalin, for example. The antibodies may also be used for in vivo diagnostic assays. Generally, the 20 antibody is labeled with a radio nuclide (such as m In, 9 Te, 4 C, 13 1, 12.5 3 H, 32 P or 3s S) so that the tumor can be localized using immunoscintiography. The antibody of the present invention can be provided in a kit, a packaged combination of reagents in predetermined amounts with instructions for performing the diagnostic assay. Where the antibody is labeled with an enzyme, the kit will include 25 substrates and cofactors required by the enzyme (e.g., a substrate precursor which provides the detectable chromophore or fluorophore). In addition, other additives may be included such as stabilizers, buffers (e.g., a block buffer or lysis buffer) and the like. The relative amounts of the various reagents- may be varied widely to provide for concentrations in solution of the reagents which substantially optimize the sensitivity of 30 the assay. Particularly, the reagents may be provided as dry powders, usually lyophilized, WO 2009/155724 PCT/CH2009/000220 48 including excipients which on dissolution will provide a reagent solution having the appropriate concentration. Pharmaceutical Preparations 5 In one aspect the invention provides pharmaceutical formulations comprising anti-VEGF antibodies for the treatment of VEGF-mediated diseases. The term "pharmaceutical formulation" refers to preparations which are in such form as to permit the biological actvity of the antibody or antibody derivative to be unequivocally effective, and which contain no additional components which are toxic to the subjects to which the 10 formulation would be administered. "Pharmaceutically acceptable" excipients (vehicles, additives) are those which can reasonably be administered to a subject mammal to provide an effective dose of the active ingredient employed. A "stable" formulation is one in which the antibody or antibody derivative therein essentially retains its physical stability and/or chemical stability and/or biological activity 15 upon storage. Various analytical techniques for measuring protein stability are available in the art and are reviewed in Peptide and Protein Drug Delivery, 247-301, Vincent Lee Ed., Marcel Dekker, Inc., New York, N.Y., Pubs. (1991) and Jones, A. Adv. Drug Delivery Rev. 10: 29-90 (1993), for example. Stability can be measured at a selected temperature for a selected time period. Preferably, the formulation is stable at room 20 temperature (about 30" C) or at 400 C for at least I week and/or stable at about 2-8' C for at least 3 months to 2 years. Furthermore, the formulation is preferably stable following freezing (to, e.g., -70" C) and thawing of the formulation. An antibody or antibody derivative "retains its physical stability" in a pharmaceutical formulation if it meets the defined release specifications for aggregation, 25 degradation, precipitation and/or denaturation upon visual examination of color and/or clarity, or as measured by UV light scattering or by size exclusion chromatography, or other suitable art recognized methods. An antibody or antibody derivative "retains its chemical stability" in a pharmaceutical formulation, if the chemical stability at a given time is such that the 30 protein is considered to still retain its biological activity as defined below. Chemical stability can be assessed by detecting and quantifying chemically altered forms of the WO 2009/155724 PCT/CH2009/000220 49 protein. Chemical alteration may involve size modification (e.g. clipping) which can be evaluated using size exclusion chromatography, SDS-PAGE and/or matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI/TOF MS), for example. Other types of chemical alteration include charge alteration (e.g. occurring as a result of 5 deamidation) which can be evaluated by ion-exchange chromatography, for example. An antibody or antibody derivative "retains its biological activity" in a pharmaceutical formulation, if the biological activity of the antibody at a given time is within about 10% (within the errors of the assay) of the biological activity exhibited at the time the pharmaceutical formulation was prepared as determined in an antigen 10 binding assay, for example. Other "biological activity" assays for antibodies are elaborated herein below. By "isotonic" is meant-that-the formulation of interest has essentially the same: osmotic pressure as human blood. Isotonic formulations will generally have an osmotic pressure from about 250 to 350 mOsm. Isotonicity can be measured using a vapor 15 pressure or ice-freezing type osmometer, for example. A "polyol" is a substance with multiple hydroxyl groups, and includes sugars (reducing and non-reducing sugars), sugar alcohols and sugar acids. Preferred polyols herein have a molecular weight which is less than about 600 kD (e.g. in the range from about 120 to about 400 kD). A "reducing sugar" is one which contains a hemiacetal group 20 that can reduce metal ions or react covalently with lysine and other amino groups in proteins and a "non-reducing sugar" is one which does not have these properties of a reducing sugar. Examples of reducing sugars are fructose, mannose, maltose, lactose, arabinose, xylose, ribose, rhamnose, galactose and glucose. Non-reducing sugars include sucrose, trehalose, sorbose, melezitose and raffinose. Mannitol, xylitol, erythritol, 25 threitol, sorbitol and glycerol are examples of sugar alcohols. As to sugar acids, these include L-gluconate and metallic salts thereof. Where it is desired that the formulation is freeze-thaw stable, the polyol is preferably one which does not crystallize at freezing temperatures (e.g. -20" C) such that it destabilizes the antibody in the formulation. Non reducing sugars such as sucrose and trehalose are the preferred polyols herein, with 30 trehalose being preferred over sucrose, because of the superior solution stability of trehalose.

WO 2009/155724 PCT/CH2009/000220 50 As used herein, "buffer" refers to a buffered solution that resists changes in pH by the action of its acid-base conjugate components. The buffer of this invention has a pH in the range from about 4.5 to about 8.0; preferably from about 5.5 to about 7. Examples of buffers that will control the pH in this range include acetate (e.g. sodium acetate), 5 succinate (such as sodium succinate), gluconate, histidine, citrate and other organic acid buffers. Where a freeze-thaw stable formulation is desired, the buffer is preferably not phosphate. In a pharmacological sense, in the context of the present invention, a "therapeutically effective amount" of an antibody or antibody derivative refers to an 10 amount effective in the prevention or treatment of a disorder for the treatment of which the antibody or antibody derivative is effective. A "disease/disorder" is any condition that would benefit from treatment with the antibody or antibody derivative. This includes chronic and acute disorders or diseases including those pathological conditions which predispose the mammal to the disorder in question. 15 A "preservative" is a compound which can be included in the formulation to essentially reduce bacterial action therein, thus facilitating the production of a multi-use formulation, for example. Examples of potential preservatives include octadecyldimethylbenzyl ammonium chloride, hexamethonium chloride, benzalkonium chloride (a mixture of alkylbenzyldimethylammonium chlorides in which the alkyl 20 groups are long-chain compounds), and benzethonium chloride. Other types of preservatives include aromatic alcohols such as phenol, butyl and benzyl alcohol, alkyl parabens such as methyl or propel paraben, catechol, resorcinol, cyclohexanol, 3 pentanol, and m-cresol. The most preferred preservative herein is benzyl alcohol. The present invention also provides pharmaceutical compositions comprising one 25 or more antibodies or antibody derivative compounds, together with at least one physiologically acceptable carrier or excipient. Pharmaceutical compositions may comprise, for example, one or more of water, buffers (e.g., neutral buffered saline or phosphate buffered saline), ethanol, mineral oil, vegetable oil, dimethylsulfoxide, carbohydrates (e.g., glucose, mannose, sucrose or dextrans), mannitol, proteins, 30 adjuvants, polypeptides or amino acids such as glycine, antioxidants, chelating agents such as EDTA or glutathione and/or preservatives. As noted above, other active WO 2009/155724 PCT/CH2009/000220 51 ingredients may (but need not) be included in the pharmaceutical compositions provided herein. A carrier is a substance that may be associated with an antibody or antibody derivative prior to administration to a patient, often for the purpose of controlling stability 5 or bioavailability of the compound. Carriers for use within such formulations are generally biocompatible, and may also be biodegradable. Carriers include, for example, monovalent or multivalent molecules such as serum albumin (e.g., human or bovine), egg albumin, peptides, polylysine and polysaccharides such as aminodextran and polyamidoamines. Carriers also include solid support materials such as beads and 10 microparticles comprising, for example, polylactate polyglycolate, poly(lactide-co glycolide), polyacrylate, latex, starch, cellulose or dextran. A carrier may bear the compounds in a variety of ways, including covalent bonding (either directly or via a linker group), noncovalent interaction or admixture. Pharmaceutical compositions may be formulated for any appropriate manner of 15 administration, including, for example, topical, intraocular, oral, nasal, rectal or parenteral administration. In certain embodiments, compositions in a form suitable for topical use, for example, as eye drops, are preferred. Other forms include, for example, pills, tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs. Within yet other 20 embodiments, compositions provided herein may be formulated as a lyophilizate. The term parenteral as used herein includes subcutaneous, intradermal, intravascular (e.g,, intravenous), intramuscular, spinal, intracranial, intrathecal and intraperitoneal injection, as well as any similar injection or infusion technique. The pharmaceutical composition may be prepared as a sterile injectible aqueous 25 or oleaginous suspension in which the modulator, depending on the vehicle and concentration used, is either suspended or dissolved in the vehicle. Such a composition may be formulated according to the known art using suitable dispersing, wetting agents and/or suspending agents such as those mentioned above. Among the acceptable vehicles and solvents that may be employed are water, 1,3-butanediol, Ringer's solution and 30 isotonic sodium chloride solution. In addition, sterile, fixed oils may be employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed, WO 2009/155724 PCT/CH2009/000220 52 including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid may be used in the preparation of injectible compositions, and adjuvants such as local anesthetics, preservatives and/or buffering agents can be dissolved in the vehicle. Pharmaceutical compositions may be formulated as sustained release formulations 5 (i.e., a formulation such as a capsule that effects a slow release of modulator following administration). Such formulations may generally be prepared using well known technology and administered by, for example, oral, rectal, or subcutaneous implantation, or by implantation at the desired target site. Carriers for use within such formulations are biocompatible, and may also be biodegradable; preferably the formulation provides a 10 relatively constant level of modulator release. The amount of an antibody or antibody derivative contained within a sustained release formulation depends upon, for example, the site;ofrimplantation, the rate and expected duration of release and'the nature of the disease/disorder to be treated or prevented. Antibody or antibody derivatives provided herein are generally administered in an 15 amount that achieves a concentration in a body fluid (e.g., blood, plasma, serum, CSF, synovial fluid, lymph, cellular interstitial fluid, tears or urine) that is sufficient to detectably bind to VEGF and prevent or inhibit VEGF-mediated diseases/disorders. A dose is considered to be effective if it results in a discernible patient benefit as described herein. Preferred systemic doses range from about 0.1 mg to about 140 mg per kilogram 20 of body weight per day (about 0.5 mg to about 7 g per patient per day), with oral doses generally being about 5-20 fold higher than intravenous doses. The amount of antibody or antibody derivative that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. Dosage unit forms will generally contain between from about I mg to 25 about 500 mg of an active ingredient. Pharmaceutical compositions may be packaged for treating conditions responsive to an antibody or antibody derivative directed to VEGF. Packaged pharmaceutical compositions may include a container holding a effective amount of at least one antibody or antibody derivative as described herein and instructions (e.g., labeling) indicating that 30 the contained composition is to be used for treating a disease/disorder responsive to one antibody or antibody derivative following administration in the patient.

WO 2009/155724 PCT/CH2009/000220 53 The antibodies or antibody derivatives of the present invention can also be chemically modified. Preferred modifying groups are polymers, for example an optionally substituted straight or branched chain polyalkene, polyalkenylene, or polyoxyalkylene polymer or a branched or unbranched polysaccharide. Such effector 5 group may increase the half-live of the antibody in vivo. Particular examples of synthetic polymers include optionally substituted straight or branched chain poly(ethyleneglycol) (PEG), poly(propyleneglycol), poly(vinvlalcohol) or derivatives thereof. Particular naturally occurring polymers include lactose, amylose, dextran, glycogen or derivatives thereof. The size of the polymer may be varied as desired, but will generally be in an 10 average molecular weight range from 50ODa to 50000Da. For local application where the antibody is designed to penetrate tissue, a preferred molecular weight of the polymer is around 500ODa. Thepolymer molecule can be attached to the antibody, in particular-to' the C-terminal end of the Fab fragment heavy chain via a covalently linked hinge peptide as described in WOO 194585. Regarding the attachment of PEG moieties, reference is 15 made to "Poly(ethyleneglycol) Chemistry, Biotechnological and Biomedical Applications", 1992, J. Milton Harris (ed), Plenum Press, New York and "Bioconjugation Protein Coupling Techniques for the Biomedical Sciences", 1998, M. Aslam and A. Dent, Grove Publishers, New York. After preparation of the antibody or antibody derivative of interest as described 20 above, the pharmaceutical formulation comprising it is prepared. The antibody to be formulated has not been subjected to prior lyophilization and the formulation of interest herein is an aqueous formulation. Preferably the antibody or antibody derivative in the formulation is an antibody fragment, such as an scFv. The therapeutically effective amount of antibody present in the formulation is determined by taking into account the 25 desired dose volumes and mode(s) of administration, for example. From about 0.1 mg/ml to about 50 mg/ml, preferably from about 0.5 mg/ml to about 40 mg/ml and most preferably from about 10 mg/ml to about 20 mg/ml is an exemplary antibody concentration in the formulation. An aqueous formulation is prepared comprising the antibody or antibody 30 derivative in a pH-buffered solution The buffer of this invention has a pH in the range from about 4.5 to about 8.0, preferably from about 5.5 to about 7. Examples of buffers WO 2009/155724 PCT/CH2009/000220 54 that will control the pH within this range include acetate (e.g. sodium acetate), succinate (such as sodium succinate), gluconate, histidine, citrate and other organic acid buffers. The buffer concentration can be from about I mM to about 50 mM, preferably from about 5 mM to about 30 mM, depending, for example, on the buffer and the desired 5 isotonicity of the formulation. A polyol, which acts as a tonicifier and may stabilize the antibody, is included in the formulation. In preferred embodiments, the formulation does not contain a tonicifying amount of a salt such as sodium chloride, as this may cause the antibody or antibody derivative to precipitate and/or may result in oxidation at low pH. In preferred 10 embodiments, the polyol is a non-reducing sugar, such as sucrose or trehalose. The polyol is added to the formulation in an amount which may vary with respect to the desired isotonicity of the formulation. Preferablythe aqueous formulation is isotonic, in which case suitable concentrations of the polyol in the formulation are in the range from about 1% to about 15% w/v, preferably in the range from about 2% to about 10% why, for 15 example. However, hypertonic or hypotonic formulations may also be suitable. The amount of polyol added may also alter with respect to the molecular weight of the polyol. For example, a lower amount of a monosaccharide (e.g. mannitol) may be added, compared to a disaccharide (such as trehalose). A surfactant is also added to the antibody or antibody derivative formulation. 20 Exemplary surfactants include nonionic surfactants such as polysorbates (e.g. polysorbates 20, 80 etc) or poloxamers (e.g. poloxamer 188). The amount of surfactant added is such that it reduces aggregation of the formulated antibody/antibody derivative and/or minimizes the formation of particulates in the formulation and/or reduces adsorption. For example, the surfactant may be present in the formulation in an amount 25 from about 0.00 1% to about 0.5%, preferably from about 0.005% to about 0.2% and most preferably from about 0.01% to about 0.1%. In one embodiment, the formulation contains the above-identified agents (i.e. antibody or antibody derivative, buffer, polyol and surfactant) and is essentially free of one or more preservatives, such as benzyl alcohol, phenol, m-cresol, chlorobutanol and 30 benzethonium Cl. In another embodiment, a preservative may be included in the formulation, particularly where the formulation is a multidose formulation. The WO 2009/155724 PCT/CH2009/000220 55 concentration of preservative may be in the range from about 0.1% to about 2%, most preferably from about 0.5% to about 1%. One or more other pharmaceutically acceptable carriers, excipients or stabilizers such as those described in Remington's Pharmaceutical Sciences 21st edition, Osol, A. Ed. (2006) may be included in the formulation provided 5 that they do not adversely affect the desired characteristics of the formulation. Acceptable carriers, excipients or stabilizers are non-toxic to recipients at the dosages and concentrations employed and include: additional buffering agents, co-solvents, antioxidants including ascorbic acid and methionine, chelating agents such as EDTA, metal complexes (e.g. Zn-protein complexes), biodegradable polymers such as 10 polyesters, and/or salt-forming counterions such as sodium. The formulations to be used for in vivo administration must be sterile. This is readily accomplished by filtration through sterile filtration membranes, prior toor following, preparation of the formulation. The formulation is administered to a mammal in need of treatment with the 15 antibody, preferably a human, in accord with known methods, such as intravenous administration as a bolus or by continuous infusion over a period of time, by intramuscular, intraperitoneal, intracerobrospinal, subcutaneous, intra-articular, intrasynovial, intrathecal, oral, topical, or inhalation routes. In preferred embodiments, the formulation is administered to the mammal by topical application of eye drops to the 20 ocular surface. For such purposes, the formulation may applied using an eye drop applicator, for example. The appropriate dosage ("therapeutically effective amount") of the antibody will depend, for example, on the condition to be treated, the severity and course of the condition, whether the antibody is administered for preventive or therapeutic purposes, 25 previous therapy, the patient's clinical history and response to the antibody, the type of antibody used, and the discretion of the attending physician. The antibody or antibody derivative is suitably administered to the patent at one time or over a series of treatments and may be administered to the patent at any time from diagnosis onwards. The antibody or antibody derivative may be administered as the sole treatment or in conjunction with 30 other drugs or therapies useful in treating the condition in question.

WO 2009/155724 PCT/CH2009/000220 56 As a general proposition, the therapeutically effective amount of the antibody or antibody derivative administered will be in the range of about 0.1 to about 50 mg/kg of patent body weight whether by one or more administrations, with the typical range of antibody used being about 0.3 to about 20 mg/kg, more preferably about 0.3 to about 15 5 mg/kg, administered daily, for example. However, other dosage regimens may be useful. The progress of this therapy is easily monitored by conventional techniques. FDA approved doeses and regimes suitable for use with Lucentis are considered. Other doses and regimes are described in U.S. Provisional Application Serial No. 61/075,64 1, entitled "Improved Immunobinder Formulations And Methods For 10 Adminstration". filed June 25, 2008, which is expressly incorporated herein. Articles of Manufacture In another embodiment of the invention, an article of manufacture is provided comprising a container which holds the aqueous pharmaceutical formulation of the 15 present invention and optionally provides instructions for its use. Suitable containers include, for example, bottles, vials, eye drop applicators and syringes. The container may be formed from a variety of materials such as glass or plastic. An exemplary container is a 3-20 cc single use glass or plastic vial. Alternatively, for a multidose formulation, the container may be 3-100 cc glass vial. The container holds the formulation and the label 20 on, or associated with, the container may indicate directions for use. The article of manufacture may further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, needles, syringes, and package inserts with instructions for use. 25 Exemplification The present disclosure is further illustrated by the following examples, which should not be construed as further limiting. The contents of all figures and all references, patents and published patent applications cited throughout this application are expressly incorporated herein by reference in their entireties. 30 Throughout the examples, the following materials and methods were used unless otherwise stated.

WO 2009/155724 PCT/CH2009/000220 57 General Materials and Methods In general, the practice of the present invention employs, unless otherwise indicated, conventional techniques of chemistry, molecular biology, recombinant DNA 5 technology, immunology (especially, e.g., antibody technology), and standard techniques of polypeptide preparation. See, e.g., Sambrook, Fritsch and Maniatis, Molecular Cloning: Cold Spring Harbor Laboratory Press (1989); Antibody Engineering Protocols (Methods in Molecular Biology), 510, Paul, S., Humana Pr (1996); Antibody Engineering: A Practical Approach (Practical Approach Series. 169), McCafferty, Ed., Irl 10 Pr (1996); Antibodies: A Laboratory Manual, Harlow et al., C.S.H.L. Press, Pub. (1999); and Current Protocols in Molecular Biology, eds. Ausubel et al, John Wiley & Sons (1992). ThermostabilitV measurements 15 Attenuated total reflectance Fourier transform IR (FTIR-ATR) spectra were obtained for various single chains and derivative molecules using the FT-IR Bio-ATR cell in a Tensor Bruker. The molecules were concentrated up to 3mg/ml and dialyzed overnight at 4'C against PBS, pH 6.5 and the buffer flow through was collected as blank. The denaturation profiles were obtained by thermo challenging the molecules with a 20 broad range of temperatures in 5"C steps (25 to 95'C). All spectra manipulations were performed using OPUS software. The main buffer and transient atmospheric (CO 2 and 1-120) background were substracted from the protein spectrum. The resulting protein spectrum was then baseline corrected and the protein amide I spectra was determined from the width of the widest resolvable peak in the expected region. Second derivative 25 spectra were obtained for the aide I band spectra using a third degree polynomial function with a smoothing function. Changes in protein structure were estimated by amide I second derivative analysis using a linear calibration curve for the initial curve-fit calculations assuming 0% denaturation for the 3 lower measurements and 100% denaturation for the 3 higher measurements. The denaturation profiles were used to 30 approximate midpoints of the thermal unfolding transitions (TM) for every variant applying the Boltzmann sigmoidal model.

WO 2009/155724 PCT/CH2009/000220 58 Solubility measurements Relative solubility of various scFv molecules was measured after enhancing protein aggregation and precipitation in presence of ammonium sulfate. Ammonium 5 sulfate was added to the protein in aqueous solutions to yield increments of 5% of saturation in the final mixture salt-protein. The precipitation in the dynamic range was determined empirically and the saturation intervals reduced in this range to 2.5% intervals saturation in the final mixture. After ammonium sulfate addition, samples were gently mixed and centrifuged 30 minutes at 6000rpm. The remaining protein in 10 supernatants was recovered for each ammonium sulfate percentage of saturation. Solubility curves were determined by measuring the protein concentration in the supernatant by UV-VIS measurements using NanoDropTM4 000 Spectrophotometer. Measurements of remaining soluble protein in supernatants were normalized and used to estimate midpoints of relative solubility for every variant applying the Boltzmann 15 sigmoidal model. Short Term Stability test The scFv molecules were examined after two weeks incubation at 40*C for the presence of soluble aggregates and degradation products. Proteins with a concentration of 20 10 mg/ml were dialyzed overnight at 4C against PBS with a broad range of pHs (3.5, 4.5, 5.5, 6.5, 7.0, 7.5 and 8.5). Control molecules with the same concentration in standard buffer PBS (pH 6.5) were stored at -80*C during the 2 weeks period. Determination of degradation bands by SDS-PAGE was done at t=0 and t=14d time points and soluble aggregates were assessed in the SEC-HPLC. Determination of remaining activity after 2 25 weeks at 40'C was done using Biacore, EXAMPLE 1 MMNIZATION STRATEGY FOR GENERATING ANTI-VEGF ANTIBODIES. In this example, an immunization strategy is described which used a novel 30 antigenic VEGF-derived peptide, to generate antibodies capable of recognizing human, mouse and rabbit VEGFA.

WO 2009/155724 PCT/CH2009/000220 59 From alanine-scanning mutagenesis studies performed at Genentech the residues of VEGFA that are crucial for high affinity interaction with VEGFr are known (Fuh, G. et al, (2006) J. BioL Chem. 281, 6625-6631). Although the receptor-binding site probably represents a conformational epitope, most of the crucial residues lie on an alpha helix, on 5 the first 10 amino acids of mature VEGFA. Rabbit VEGFA contains three amino acids changes in this alpha helix, when compared to the human sequence; in contrast, mouse VEGFA is identical to human in this region. Thus, for the generation of mouse-human cross-reactive antibodies, rabbit presents a suitable species for immunization. In addition, rabbit immunization can lead to 10 Abs with higher affinity than mouse immunization. As outlined above, interaction with residues on the N-terminal alpha helix of VEGFA-seems to be most crucial for binding to VEGFRI. Thereforethis 10 amino acid long stretch can be used as an epitope for immunization. Alternatively, full length VEGFA can be injected, however, other peptide stretches on VEGFA are more 15 immunogenic, thus lowering the chance to raise neutralizing antibodies. This hypothesis is supported by the fact that two different peptides, both lying close to the C-terminus of VEGFA are potentially immunogenic as predicted by the method of Johnson and Wolf. This method predicts only minor immunogenic potential for the N-terminal alpha helix. Therefore, immunization with the peptide constituting the alpha helix only, can be more 20 straightforward than immunization with full-length VEGFA. The probability to elicit a strong immune response can be further increased by fusion or chemical coupling of the peptide to Keyhole Limpet Hemocyanin (KLH). Four immunization strategies were performed as follows A. Pre-Immunization of rabbits with full-length human VEGFA 1 6 5 to enhance the 25 probability to obtain conformational binders. Second boost with peptide from aa stretch 16-KFMDVYQRsYCHP-28 (underline: receptor interaction; double underline, divergent in rabbit, Cys is involved in disulfide bond according to crystal structure). The Cys contained in the peptided sequence could be used for coupling to KLH and would therefore not be exposed as free Cys. The final peptide would look as follows: 30 KFMDVYQRSY-Cys-KLH.

WO 2009/155724 PCT/CH2009/000220 60 B. Pre-Immunization of mice with full-length VEGFA1 65 to enhance the probability to obtain conformational binders. Second boost with peptide from aa stretch 16 KFMvYQRSYCHP -28 (Cys is involved in disulfide bond according to crystal structure). The Cys contained in the peptided sequence can be used for coupling to KLH and would 5 therefore not be exposed as free Cys. The final peptide would look as follows: KFMDVYQRSY-Cys-KLH. C. Pre-immunization of rabbits/mice with peptide from aa stretch 16 KFMDvYQRSYCHP -28 (final peptide: KFMDVYQRSY-Cys-KLH). Second boost with full-length VEGFA 165 to enhance the probability to obtain conformational binders. 10 1). Immunization with full length VEGFA 1 6 s in rabbits. EXAMPLE 2 CDR Grafting and Functional Humanization of monoclonal rabbit anti-VEGF antibodies. 15 jGraftingof Rabbit CDRs Unlike traditional humanization methods which employ the human antibody acceptor framework that shares the greatest sequence homology with the non-human donor antibody, the rabbit CDRs were grafted into either framework FW 1.4 (SEQ ID No. 172) to generate a Min-graft or into the "rabbitized" framework rFW1.4 (SEQ ID No. 20 173) or its variant rFW 1 .4(v2) (SEQ ID No. 174) to generate a Max-graft. Both frameworks were selected primarily for desirable functional properties (solubility and stability), structural suitability to accommodate a large variety of rabbit CDRs and reasonable homology to the rabbit variable domain consensus sequence. Framework rFW 1.4 is a derivative of FWI1.4 that was further engineered with the aim to serve as 25 universal acceptor framework for virtually any set of rabbit CDRs. Although the stable and soluble framework sequence FW1.4 exhibits high homology to rabbit antibodies, it is not the most homologous sequence available. Identification o residues potentially involved in binding For each rabbit variable domain sequence, the nearest rabbit germline counterpart 30 was identified, If the closest germline could not be established, the sequence was compared against the subgroup consensus or the consensus of rabbit sequences with a WO 2009/155724 PCT/CH2009/000220 61 high percentage of similarity. Rare framework residues were considered as possible result of somatic hypermutation and therefore playing a role in antigen binding. Consequently, such residues were considered for grafting onto the acceptor framework rFW1.4 or rFW1.4(v2) to generate Max-grafts. Particularly, residues potentially implicated in direct 5 antigen contact or influencing disposition of VL and VH were grafted. Further residues described to influence CDR structure were substituted if required. No framework substitutions were made when CDRs were grafted onto FW 1.4 (Min-grafts). For example to generate 578minmax residue VH 94 (H94) of rFW1.4 was mutated to corresponding residue in the donor sequence. The rabbit antibody 578 contains Gly at H94 whereas 10 both, the most homologous germline and the rabbit consensus contain Arg at position H94. Gly has an exceptional flexibility (positive phi angles) that is not found for other amino acids. This suggests a role in:mainchain torsion angle and a possible strong influence of the loop conformation with implications on activity. Further examples of framework positions that were grafted to obtain the Max-grafts as disclosed herein can be 15 identified by making a sequence alignment of the framework regions of rFW 1.4, rFW 1.4(v2) and the scFv sequences of interest provided herein. Webtools as known in the art may for example be used for said purpose (e.g. ClustalW as available on June 23, 2009 at http://www.ebi.ac.uk/Tools/clustalw2/index.html or MultiAlin as avialable on June 23, 2009 at http:/Ibioinfo.genotoul.fr/mnultalin). All framework positions at which 20 rFW 1.4 and rFW1.4(v2) contain the same residue and at which the scFv of interest reveals a different residue, are framework positions that were grafted to obtain the Max grafts. Domain shuffling Variable light chains of Min-grafts were combined with variable heavy chain 25 Max-grafts to identify optimal combinations in terms of biophysical properties (solubility and stability) and activity. Cloning and expressionofscFvs The scFvs described and characterized herein were produced as follows. The humanized VL sequences (SEQ ID NOs:82-106) were connected to humanized VH 30 sequences (SEQ ID NOs: 118-166) via the linker of SEQ ID NO: 181 to yield an scFv of the following orientation: NH 2 -VL-linker-VH-COOH. In many cases DNA sequences WO 2009/155724 PCT/CH2009/000220 62 encoding for the various scFvs were de novo synthesized at the service provider Entelechon GmbH (www.entelechon.com). The resulting DNA inserts were cloned into the bacterial expression vector pGMP002 via Neol and HindIII restriction sites introduced at the 5' and 3' end of the scFv DNA sequence, respectively. Between the 5 DNA sequence of theVL domain and the V-J domain, a BamHI restriction site is located. In some cases the scFv encoding DNA was not de novo synthesized, but the scFv expressing constructs were cloned by domain shuffling. Accordingly, the VL domains were excised and introduced into the new constructs via Ncol and BamHI restriction sites, the VII domains via BamHI and HindiII restriction sites. In other cases, point 10 mutations were introduced into the VH and/or VL domain using state of the art assembling PCR methods. The cloning of GMP002 is described in Example I of W02008006235. The production of the scFvs was done analogue as for ESBA 105 as described in Examplel of W02008006235. 15 EXAMPLE 3 BIACORE BINDING ANALYSIS OF ANTI-VEGF SCFVS In this example, the Biacore-binding ability of scFvs was tested and the binding affinity was measured using the exemplary surface plasmon resonance method with 20 BlAcore M-TI00. The VEGF proteins, tested for binding by these scFv candidates, in this example and later examples include purified Escherichia coli-expressed recombinant human VEGF 165 (PeproTech EC Ltd.), recombinant human VEGF 121 (PeproTech EC Ltd.), recombinant human VEGF no (ESBATech AG), recombinant murine VEGF 1 6 4 (PeproTech EC Ltd.), recombinant rat VEGFI64 (Biovision), recombinant rabbit VEGF o 25 (ESBATech AG), and recombinant human PLGF (PeproTech EC Ltd.). For the surface plasmon resonance experiment, carboxymethylated dextran biosensor chips (CM4, GE Flealthcare) were activated with N-ethyl-N'-(3-dimethylam inopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide according to the supplier's instructions. Each of the 6 different VEGF forms, as exemplified above, was coupled to I of the 4 different 30 flow cells on a CM4 sensor chip using a standard amine-coupling procedure. The range of responses obtained with these immobilized VEGF molecules after coupling and WO 2009/155724 PCT/CH2009/000220 63 blocking were ~250-500 response units (RU) for hVEGF 6 , ~200 RU for hVEGFJ t, hVEGF 2 m, murine VEGF 1 64, rat VEGFi4 and rabbit VEGF oo and ~400 RU for PLGF. The 4th flow cell of each chip was treated similarly except no proteins were immobilized prior to blocking, and the flow cell was used as in-line reference. Various concentrations 5 of anti-VEGF scFvs (e.g., 90 nM, 30 nM, 10 nM, 3.33 nM, 1.11 nM, 0.37 nM, 0.12 nM and 0.04 nM) in HBS-EP buffer (0.01 M HEPES, pl 7.4 or 5, 0.15 M NaCl, 3 mM EDTA. 0.005% surfactant P20) were injected into the flow cells at a flow rate of 30 pA/min for 5 min. Dissociation of the anti-VEGF scFv from the VEGF on the CM4 chip was allowed to proceed for 10 min at 25 'C. Sensorgrams were generated for each anti 10 VEGF scFv sample after in-line reference cell correction followed by buffer sample subtraction. The apparent dissociation rate constant (kd), the apparent association rate o&htant (ka) and the apparent dissociation equilibrium constarnif(Kb) were calculated using one-to-one Langmuir binding model with BlAcore T100 evaluation Software version 1.1 15 As one exemplary result, some lead anti-VEGF scFv candidates are listed in Table 7 showing their binding affinity to hVEGF 165 . Their potency as VEGF inhibitors, which is measured using VEGFR competition ELISA and/or HUVEC assay and described in latter examples, is also shown in Table 7. The kinetics curves of some exemplary lead candidates, e.g., 511 max and 578max, for their binding to hVEGF 165 are 20 illustrated in Figure 1. Their affinity constants (kd, ka and K1) were also determined. Some lead candidates also display species specificity in their binding to various VEGF proteins of different sources. For example, some affinity data measured at pH5 using mouse and rat VEGF164 as binding partner are shown in Tables 8 a and b. An exemplary lead scFv candidate, 578minmax, has a K 0 of 5.76E-10 M and 7.48E-10 M in its binding 25 to mouse and rat VEGF164, respectively at a pH- of 5 (Tables 8 a and b) and 2,73E-1 I and 2,19E-1 I at a pH of 7,4 (data not shown). This species specificity is further illustrated in Figure 4 in the kinetics curves and affinity data for the binding between 578minmax and human, mouse or rat VEGF proteins. Besides the species specificity in their binding to VEGFs from different 30 organisms, many lead scFv candidates also display differentiated binding affinities towards various VEGF isoforms. For example, the affinity data measured at pH 5,0 for WO 2009/155724 PCT/CH2009/000220 64 some scFv candidates binding to human VEGFi 65 , VEGF 21 and VEGF 110 are compared in Table 9. In the same experiments, PIGF protein was also used as a negative control without binding capacity to those scFv candidates. Also, the differentiated kinetics curves and affinity data for the binding between 578Max and VEGF isoforms, as an example, 5 are illustrated in Figure 3. The present invention also discloses derivatives originating from the lead anti VEGF scFv candidates, which are mentioned above. Some lead derivatives of candidate 578 and 511, as listed in Table 10, are exemplified for their affinity and potency (measured at pH 5,0). In this experiment, Biacore measurement was used for the affinity 10 of these derivatives towards hVEGF 1 6 5, while hVEGFR2 competition ELISA and/or HUVEC assay were used to define their potency to inhibit VEGFs (Table 10). Three derivatives, 578max, 57.8minmax and 578 wt-His, are further exemplified in theirkinetics curves and affinity data for binding to hVEGF 165 in Figure 4. For derivatives of lead candidates, their biophysical characterizations were 15 determined and exemplified in Figures 5-7 and table 11. These characteristics include, as exemplified in table I1, Tm determined by FTIR, the percentage of p-sheet or protein loss after incubation at 60*C for 30 min, solubility determined by ammonium sulfate precipitation, refolding yield during the production process and expression levels in E. coli. Three derivatives, 578max, 578minmax and 578minmaxDHP, were characterized 20 for their thermal stability in their unfolding curves against different temperatures measured by FT-IR (Figure 5).

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As in Figure 7, the 5 percentage of soluble proteins of these derivatives under various concentrations of ammonium sulfate were compared. Table 12a : anti-VEGF binders after incubation for 30 min at 50*C Sample name Beta sheet % Nanodrop (mg/ml) 950 100,8 81,2 978 100,9 85,1 980 99.9 1003 991 99,4 99,2 -----.--.--.---------. . --------------- - -- ------------------------ __________-__-_-__----.. . 802 100,4 96,7 821 100,6 93,5 903 99,5 99,4 961 98,7 101,7 997 99,9 76,39 10 Table 12a : anti-VEGF binders after incubation for 30 min at 60*C Sample name Beta sheet % Nanodrop (mg/ml) 950 45,9 2 S102,3 .52 980 100,8 61 991 99,9 80 -_------------ -- ---- - _ _-------------------_ _ 802 101,5 96 821 101,9 84 903 100,5 89 961 100,1 85 997 49,1 2 WO 2009/155724 PCT/CH2009/000220 76 Table 12a : anti-VEGF binders after incubation for 30 min at 70 0 C Sample name Beta sheet % -Nanodrop (mg/ml) 950 43,1 1,0 978 13,4 2,7 980 4,5 0,2 991 21,5 1,4 802 100,4 80,8 82 ------------------------------ -- _ _ _ _ _ _ _ _ 821 58,41 3,3 903 81,9 0,7 961 46,3 1,1 997 0,0 0,3 EXAMPLE 4 5 VEGF RECEPTOR BLOCKING ASSAYS For anti-VEGF scFv candidates or their derivatives disclosed in the present invention, their potency as VEGF inhibitors was also measured besides their binding affinity to VEGFs in Example 3. The methods to measure their potency include, for example, the VEGFR competition ELISA, as exemplified in this example, and HUVEC 10 assays (Figure 8). The VEGFR competition ELISA assays include, for example, VEGFR2 Receptor blocking assays and VEGFRI Receptor blocking assays. For VEGFR2 Receptor blocking assay, human VEGF1 65 was coated on a 96-well Maxisorp ELISA plate (Nunc) at 0.05 ig/ml in PBS and blocked using PBS with 0.1% BSA and 0.2% Tween 20 15 (PBST). 500 ng/ml recombinant human VEGFR2/Fc chimera (R&D Systems Inc.), consisting of amino acid residues 1-764 of the extracellular domain of human VEGFR2 fused to a 6x histidine tagged Fe of human IgG 1 , was first incubated with 3-fold serially diluted anti-VEGF scFvs in PBST. After 30-60 min of incubation at room temperature, the mixtures were transferred to the human VEGF16 5 immobilized plate and incubated for 20 90 min. Binding of the VEGFR2/Fc chimera to the immobilized VEGFs 65 was detected with goat (Fab 2 ) anti-human IgG Fcy coupled to horseradish peroxidase (Jackson WO 2009/155724 PCT/CH2009/000220 ImmunoResearch) followed by substrate (BM Blue POD substrate, Roche Diagnostics). Optical density at 450 nm (OD 450 nm) was measured using a Sunrise rnicroplate reader (Tecan). Data were analyzed using a 4-parameter logistic curve fit, and ECso values were calculated from the dose-response curves of the scFvs. The exemplary potency of lead 5 candidates or their derivatives, measured by VEGFR2 Receptor blocking assay, is listed in Table 7 and 9. For VEGFRI Receptor blocking assay, human VEGF 165 was coated on a 96-well Maxisorp ELISA plate (Nune) at 0.0125 ptg/ml in PBS and blocked using PBS with 0.4% BSA and 0.1% Tween 20. 100 ng/ml of recombinant human VEGFRI/Fe chimera (R&D 10 Systems Inc.), consisting of amino acid residues 1-687 of the extracellular domain of human VEGFRI fused to a 6x histidine tagged Fe of human IgG 1 , was first incubated with 3-fold serially diluted anti-VEGF scFvs in PBST. After 30-60 min of incubation at room temperature, the mixtures were transferred to the human VEGF165 immobilized plate and incubated for 90 min. Binding of the VEGFRI/Fc chimera to the immobilized 15 VEGF 165 was detected with goat (Fab 2 ) anti-human IgG Fcy coupled to horseradish peroxidase (Jackson ImmunoResearch) followed by substrate (BM Blue POD substrate, Roche Diagnostics). Optical density at 450 nm (OD 450 nm) was measured using a Sunrise microplate reader (Tecan). Data were analyzed as above, and EC 50 values were calculated from the dose-response curves of the scFvs. The exemplary potency of lead 20 candidates, measured by VEGFRI Receptor blocking assay, is listed in Table 7. EXAMPLE 5 HUVEC ASSAY OF VEGF INHIBITION This example exemplifies HUVEC assays as another method to measure the 25 potency of the disclosed anti-VEGF scFv candidates, or their derivatives, as VEGF inhibitors. Human umbilical vein endothelial cells (HUVECs) (Promocell), pooled from several donors, were used at passage 2 to passage 14. Cells were seeded at 1000 cells/well in 50 p complete endothelial cell growth medium (ECGM) (Promocell), that 30 contained 0.4% ECGS/H, 2% Fetal Calf Serum, 0.1 ng/ml Epidermal Growth Factor, I pg/ml Hydrocortison, I ng/ml basic Fibroblast Factor and 1% penicillin/streptomycin WO 2009/155724 PCT/CH2009/000220 78 (Gibco). 7 to 8 h later, 50 il starving medium (ECGN4 without supplements containing 0.5% heat inactivated FCS and I% penicillin/streptomycin) was added to the cells and the cells were starved for 15 to 16 hours. 3 fold Serial dilutions of anti-VEGF scFvs (0.023 150 nM) and one of the following -recombinant human VEGF 165 (0.08 nM), recombinant 5 mouse VEGFis4 (0.08 nM), or recombinant rat VEGF 64(0.3 nM)-were prepared in starving medium and preincubated for 30-60 min at room temperature. The different concentrations of VEGFs were used to compensate for their different relative biological activities. Concentrations that stimulate submaximal VEGF induced proliferation (EC 90 ) were used. 100 p1 of the mixtures were added to the 96-well tissue-culture plates 10 containing the HUVEC suspension and incubated for 4 days in a 37 *C/5% CO 2 humified incubator. Proliferation of HUVECs was assessed by measuring absorbance at 450 nm (620 nm used"as-reference wavelength) after addition of 20 pl/well WSTIl dell proliferation reagent (Roche) using a Sunrise microplate reader (Tecan). Data were analyzed using a 4-parameter logistic curve-fit, and the concentration of anti-VEGF 15 scFvs required to inhibit HUVEC proliferation by 50% (ECso) was derived from inhibition curves. The exemplary potency of lead candidates or their derivatives, measured by HUVEC assays, is listed in Table 7. Further, the inhibition of hVEGFi 65 -induced HUVEC proliferation by one derivative of lead candidates, 578minmax, is exemplified in 20 Figure 9. EC50 of 578minmax for inhibition of hVEGF 165 -induced cell-proliferation is determined to be 0.06 nM (Figure 9). The potency of 578minmax as a VEGF inhibitor is about 1.6 times better compared to Lucentis. The inhibition of mouse or rat VEGF 1 64 induced HUVEC proliferation by 578minmax is also exemplified in Figure 10. EC50 of 578minmax for inhibition of mouse and rat VEGFis4 induced cell-proliferation is 0.06 25 nM and 0.07 nM, respectively (Figure 10). Thus, mouse and rat VEGF are equipotent to human VEGF for being inhibited by the exemplary derivative (578minmax). Also in this experiment, Lucentis does not inhibit proliferation induced by rodent VEGF. EXAMPLE 6 30 EFFECTS OF ANTI-VEGF SCFVS ON HVEGF 165 INDUCED VASCULAR PERMEABILITY IN HAIRLESS GUINEA PIGS WO 2009/155724 PCT/CH2009/000220 79 In this example, the effect of anti-VEGF scFvs on human VEGF1 65 induced vascular permeability was assessed in guinea pigs using the Miles assay. Thirty application sites per animal were marked on the dorsum of hairless male guinea pigs using a permanent marker. On the treatment day each animal was administered 5 intravenously with I ml of a 1% Evans blue dye solution under general anesthesia. One hour after dye injection, 0.1 ml of test solution containing 2.61 nM recombinant human

VEGF

1 65 (PeproTech EC Ltd.) and various concentrations of anti-VEGF scFvs (0 nM, 0.085 nM, 0.256 nM, 0.767 nM, 2.3 nM, 6.9 nM, 20.7 nM, 62.1 nM; n = 7 animals per test item) was injected in triplicate into the marks on the dorsum (3 injections per 10 concentration of test item). Injections of PBS served as a negative control in all animals. As an additional control, 6.9 nM Lucentis (Novartis) was injected in all animals. One hour after injection of the test solutions, the animals were euthanized, and the pelts were collected, cleaned, and photographed digitally using incident and transmitted light. The area of Evans Blue dye that extravasated into the injection sites was evaluated 15 using ImageJ. For each animal, anti-VEGF scFv concentration versus area of dye leakage was analyzed using a 4-parameter logistic curve fit. The concentration of anti-VEGF scFvs required to inhibit vascular leakage by 50% (ECso) was derived from inhibition curves. The experiment protocol is exemplified in Figure 11. Also, the efficacy of scFv 20 candidates, ESBA903 (578minmax) and 802 (51 imax), in inhibiting the hVEGF was illustrated in Figure 11, represented by different sizes of areas containing the Evans Blue dye leaked from vascular system into skin. The efficacy data for 903 and 802 are shown in Figure 12. At 6.9 nM, 903 and 802 showed stronger inhibition of VEGF induced vascular leakage into the skin compared to Lucentis in all animals tested (Figure 12). 25 EXAMPLE 7 EFFECTS OF TOPICAL ANTI-VEGF SCFVS TREATMENT ON HVEGF 65 INDUCED RETINAL VASCULAR LEAKAGE IN RATS In this example, topical efficacy of 578minmax is demonstarted using a modified 30 Miles assay. These modifications include, for example, premixed study with intravitreal injections and topical application of scFvs.

WO 2009/155724 PCT/CH2009/000220 80 Premixed different concentrations of anti-VEGF scFv (10, 3, and I fold molar excess over VEGF) and VEGF (500 ng) were applied via a single intravitreal injection. Avastin (Roche) (10, 3, and I fold molar excess over VEGF) was used as a positive control. Vehicle for 578minmax (Citrate Buffer, 20 mM Na-Citrate, 125 mM NaCl, p-I 5 7) was used as negative control. As illustrated in Figure 13, premixing with hVEGF 165 faciliated 578minmax (ESBA903) to completely inhibit hVEGF-induced retinal vascular permeability. In this experiment, the inhibitory effect of 578minmax (ESBA903) was more significant compared to Avastin. For topical application, five days before VEGF stimulation, adult Sprague 10 Dawley rats received 578minmax (1% = 10 mg/ml) via bilateral topical dosing qid (4 drops/day) till perfusion day (Day 6). Vehicle for 578minmax (topical dosing) and Alcon RTKi (10 mg/kg/d, oral gavage) were-used as negative and positive controls. On Day 5, rats are anesthetized and their pupils are dilated. All animals receive intravitreal injections of 500 ng hrVEGF (10 pl) in both eyes. Following 24 hours post 15 injection of VEGF, intravenous infusion of 3% Evans blue dye is performed on all animals during general anesthesia. After the dye has circulated for 90 minutes, the rats are euthanized. Blood samples are taken, then the rats are perfused with sterile saline solution, then both eyes of each rat are immediately enucleated and the retinas harvested using a surgical microscope. For both retina and plasma samples, 60 p.L of supernatant is 20 used to measure the Evans blue dye absorbance (ABS) with a spectrophotometer at 620/740 nm. The blood-retinal barrier breakdown and subsequent retinal vascular permeability as measured by dye absorbance are calculated as means ± s.e.m. of net ABS/wet weight/plasma ABS. One way ANOVA is used to determine an overall difference between treatment means, where P 5 0.05 is considered significant. As 25 exemplified in Figure 14, the topical administration (5 days of pretreatment, 4 drops per day) of 578minmax (903) significantly inhibited hVEGF-induced retinal vascular permeability. This is the first demonstration of a topically effective antibody useful for the treatment of intraocular disease.

WO 2009/155724 PCT/CH2009/000220 81 .EQUIVALENTS Numerous modifications and alternative embodiments of the present invention will be apparent to those skilled in the art in view of the foregoing description. Accordingly, this description is to be construed as illustrative only and is for the purpose 5 of teaching those skilled in the art the best mode for carrying out the present invention. Details of the structure may vary substantially without departing from the spirit of the invention, and exclusive use of all modifications that come within the scope of the appended claims is reserved. It is intended that the present invention be limited only to the extent required by the appended claims and the applicable rules of law. 10 All literature and similar material cited in this application, including, patents, patent applications, articles, books, treatises, dissertations, web pages, figures and/or appendices, regardless of the format of such literature-and similar materials, are expressly incorporated by reference in their entirety. In the event that one or more of the incorporated literature and similar materials differs from or contradicts this specification, 15 including defined terms, term usage, described techniques, or the like, this specification controls. The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described in any way. While the present inventions have been described in conjunction with various 20 embodiments and examples, it is not intended that the present teachings be limited to such embodiments or examples. On the contrary, the present inventions encompass various alternatives, modifications, and equivalents, as will be appreciated by those of skill in the art. The claims should not be read as limited to the described order or elements unless 25 stated to that effect. It should be understood that various changes in form and detail may be made without departing from the scope of the appended claims. Therefore, all embodiments that come within the scope and spirit of the following claims and equivalents thereto are claimed.

WO 2009/155724 PCT/CH2009/000220 82 SEQUENCE LISTING SEQ ID NO:1 Peptide Immunogen 5 KFMDVYQRSYC VL sequences: SEQ ID NO. 72: 60 10 EVVMAQTPASVEAAVGGTVTIKCQASQSISSYLSWYQQKPGQPPKLLYKASTLASGVPSRFKGSRS GTEYTLTISDLECADAATYYCQSNYGGSSSDYGNPFGGGTEAVVK SEQ ID NO. 73: 435 AFELTQTPSSVEAAVGGTVTIKCQASQSIGSSLAWYQQKPGQRPKLLIYTAANLASGVPSRFRGSRSG 15 AAFTLTISDLECADAATYYCQN FATSDTVTFGGGTEVVVT SEQ ID NO. 74: 453 AVVLTQTPSPVSAAVGGTVSISCQSSQSVWNNNRLAWFQQKSGQPPKLLIYYASTLASGVPSR FKG SGSGTEFTLTISDVQCDDAATYYCAGGYSSTSDNTFGGGTEVVVK 20 SEQ ID NO. 75: 375 DIVMTQTPASVEATVGGTITINCQASENINIWLSWYQQKPGQPPKLLIYQASKLASGVPSRFKGSGS GTQFTLTISDLECADAAIYYCQNNYSYNRYGAPFGGGTEVVVK 25 SEQ ID NO. 76: 610 DVVMTQTPASVSE PVGGTVTI KCQASQSISSWLSWYQQKPGQP PKLLYQASTLASGVPPRSSGSG SGTEYTLTISDLECADAATYFCQNNYGFRSYGGAFGGGTEVVVK SEQ ID NO.77: 578 30 DVVMTQTPSSVSAAVGDTVTINCQASEIIHSWLAWYQQKPGQPPKLLIYLASTLASGVPSRFKGSGS GTQFTLTISDLECADAAIYYCQNVYLASTNGANFGGGTEVVVK SEQ ID NO. 78: 534 DVVMTQTPSSVSAAVGDTVTIKCQASQSINIWLSWYQQKSGQPPKLLVYKESTLASGVPSR FRGSG 35 SGTQFTLTISDLECADAATYYCQNNYDSGNNGFPFGGGTEVVVK SEQ ID NO. 79: 567 DVVMTQTPSSVSAAVGDTVTINCQADQSIYIWLSWYQQKPGQPPKLLYKASTLESGVPSRFKGSGS GTQFTLTISDLECADAATYYCQNNAHYSTNGGTFGGGTEVVVK 40 SEQ ID NO. 80: 509 DVVMTQTPSSVSAAVGDTVTIKCQASQNIRIWLSWYQQKPGQPPKLLYKASTLESGVPSRFKGSGS

GTEFTLTISDLECADAATYYCQNNAHYSTNGGTFGGGTEVVVK

WO 2009/155724 PCT/CH2009/000220 83 SEQ ID NO. 81: 511 EVVMTQTPASVEAAVGGTVTIKCQASQSINIWCSWYQQKPGHPPKLIYRASTLASGVSSRFKGSGS GTE FTLTIS DLECADAATYYCQANYAYSAGYGAAFGGGTEVVVK 5 SEQ ID NO. 82: 60min EIVMTQSPSTLSASVGDRVIITCQASQSISSYLSWYQQKPGKAPKLLIYKASTLASGVPSRFSGSGSG~A EFTLTISSLQPDDFATYYCQSNYGGSSSDYGNPFGQGTKLTVLG SEQ ID NO. 83: 435min 10 EIVMTQSPSTLSASVGDRVIITCQASQSIGSSLAWYQQKPGKAPKLLYTAANLASGVPSRFSGSGSG A EFTLTISSLQPDD FATYYCQN FATSDTVTFGQGTKLTVIG SEQ ID NO. 84: 453min EIVMTQSPSTLSASVGDRVIITCQSSQSVWNNNRLAWYQQKPGKAPKLL.IYYASTLASGVPSRFSGS 15 GSGAEFTLTISSLQPDDFATYYCAGGYSSTSDNTFGQGTKLTVLG SEQ ID NO. 85: 375nIr EIVMTQSPSTLSASVGDRVI1TCQASENINIWLSWYQQKPGKAPKLLIYQASKLASGVPSRFSGSGSG AEFTLTISSLQPDDFATYYCQNNYSYNRYGAPFGQGTKLTVLG 20 SEQ ID NO. 86: 610min EIVMTQSPSTLSASVGDRVIITCQASQSISSWLSWYQQKPGKAPKLLIYQASTLASGVPSRFSGSGSG AEFTLTISSLQPDDFATYYCQNNYGFRSYGGAFGQGTKLTVLG 25 SEQ ID NO. 87: 578min EIVMTQSPSTLSASVGDRVIITCQASEIIHSWLAWYQQKPGKAPKLLIYLASTLASGVPSRFSGSGSGA EFTLTISSLQPDDFATYYCQNVYLASTNGANFGQGTKLTVLG SEQ ID NO. 88: 534min 30 EIVMTQSPSTLSASVGDRVIITCQASQSINIWLSWYQQKPGKAPKLLIYKESTLASGVPSRFSGSGSGA EFTLTISSLQPDDFATYYCQNNYDSGNNGFPFGQGTKLTVLG SEQ ID NO. 89: 567min EIVMTQSPSTLSASVGDRVIITCQADQSIYIWLSWYQQKPGKAPKL LIYKASTLESGVPSRFSGSGSGA 35 EFTLTISSLQPDDFATYYCQNNAHYSTNGGTFGQGTKLTVLG SEQ ID NO. 90: 509min EIVMTQSPSTLSASVGDRVIITCQASQNIRIWLSWYQQKPGKAPKLLIYKASTLESGVPSRFSGSGSG AEFTLTISSLQPDDFATYYCQNNAHYSTNGGTFGQGTKLTVLG 40 SEQ ID NO, 91: S11min EIVMTQSPSTLSASVGDRVIITCQASQSINIWCSWYQQKPGKAPKLLIYRASTLASGVPSRFSGSGSG AE FTLTISSLQPDDFATYYCQANYAYSAGYGAAFGQGTKLTVLG WO 2009/155724 PCT/CH2009/000220 84 SEQ ID NO. 92: 578min Pref subst EIVLTQSPSSLSASVGDRTITCQASEIIHSWLAWYQQRPGKAPKLLISLASTLASGVPSRFSGSGSGT DFTFTISSLQPEDFAVYYCQNVYLASTNGANFGQGTKVEIKR 5 SEQ ID NO. 93: 60max EIVMTQSPSTLSASVGDRVIITCQASQSISSYLSWYQQKPGKAPKLLIYKASTLASGVPSRFSGSGSGT EFTLTISSLQPDDFATYYCQSNYGGSSSDYGNPFGQGTKLTVLG SEQ ID NO. 94: 435max 10 EIVMTQSPSTLSASVGDRVIIKCQASQSIGSSLAWYQQKPGKAPKLLIYTAANLASGVPSRFSGSGSG AE FTLTISS LQPD DFATYYCQN FATSDTVTFGQGTKLTVLG SEQ ID NO. 95: 453max EIVMTQSPSTLSASVGDRVIITCQSSQSVWNNNRLAWYQQKPGKAPKLLIYYASTLASGVPSRFSGS 15 GSGTEFTLTISSLQPDDFATYYCAGGYSSTSDNTFGQGTKLTVLG SEQ ID NO. 96: 375max EIVMTQSPSTLSASVGDRVIITCQASENINIWLSWYQQKPGKAPKLLIYQASKLASGVPSRFSGSGSG TQFTLTISSLQPDDFATYYCQNNYSYNRYGAPFGQGTKLTVLG 20 SEQ ID NO. 97: 610max EIVMTQSPSTLSASVGDRVIITCQASQSISSWLSWYQQKPGKAPKLLIYQASTLASGVPSRFSGSGSG TE FTLTISSLQPDDFATYYCQNNYGFRSYGGAFGQGTKLTVLG 25 SEQ ID NO, 98: 578max EIVMTQSPSTLSASVGDRVIITCQASEIIHSWLAWYQQKPGKAPKLLlYLASTLASGVPSRFSGSGSGT QFTLTISSLQPDDFATYYCQNVYLASTNGANFGQGTKLTVLG SEQ ID NO. 99: 534max 30 EIVMTQSPSTLSASVGDRVIlTCQASQSINIWLSWYQQKPGKAPKt-LYKESTLASGVPSRFSGSGSGT EFTLTISSLQPDDFATYYCQNNYDSGNNGFPFGQGTKLTVLG SEQ ID NO. 100: 567max EIVMTQSPSTLSASVGDRVIITCQADQSIYIWLSWYQQKPGKAPKLIYKASTLESGVPSRFSGSGSGT 35 QFTLTISSLQPDDFATYYCQNNAHYSTNGGTFGQGTKLTVLG SEQ ID NO. 101: 509max EIVMTQSPSTLSASVGDRVIITCQASQNIRIWLSWYQQKPGKAPKLLIYKASTLESGVPSRFSGSGSGT EFTLTISSLQPDDFATYYCQNNAHYSTNGGTFGQGTKLTVLG 40 SEQ ID NO. 102: 511max EIVMTQSPSTLSASVGDRVIITCQASQSINIWLSWYQQKPGKAPKLLIYRASTLASGVPSRFSGSGSGT

EFTLTISSLQPDDFATYYCQANYAYSAGYGAAFGQGTKLTVLG

WO 2009/155724 PCT/CH2009/000220 85 SEQ ID NO. 103: 578max Pref subst EIVMTQSPSSLSASVGDRVTITCQASEIIHSWLAWYQQRPGKAPKLLISLASTLASGVPSRFSGSGSGT QFTFTISSLQPEDFAVYYCQNVYLASTNGANFGQGTKVEIKR 5 SEQ ID NO. 104: 578min VL: E1D DIVMTQSPSTLSASVGDRVI ITCQASEIIHSWLAWYQQKPGKAPKLLIYLASTLASGVPSRFSGSGSGA EFTLTISSLQPDDFATYYCQNVYLASTNGANFGQGTKLTVLG SEQ ID NO. 105: 578min VL: 12V 10 EVVMTQSPSTLSASVGDRVIITCQASEIIHSWLAWYQQKPGKAPKLLYLASTLASGVPSRFSGSGSG AEFTLTISSLQPDDFATYYCQNVYLASTNGANFGQGTKLTVLG SEQ ID NO. 106: 511min VL: C41L EIVMTQSPSTLSASVGDRVIITCQASQSINIWLSWYQQKPGKAPKLLIYRASTLASGVPSRFSGSGSG 15 AEFTLTISSLQPDDFATYYCQANYAYSAGYGAAFGQGTKLTVLG VH sequences: 20 SEQ ID NO. 107: 60-11-4 QSLEESGGDLVKPGASLTLTCTASGFPFSSGYWVCWVRQA PG KGLEWIACIYAGSSGSTYYASWAK GRFTISKTSSTTVTLQMTSLTAADTATYFCARG N NYYIYTDGGYAYAG LELWG PG I LVTVSS SEQ ID NO. 108: 60-11-6 25 QSLEESGGDLVKPGASLTITCTASGFSFSSGYWICWVRQAPGKGLEWIACIYAGSSGSTYYASWAKG RFTISKTSSTTVTLQMTSLTAADTATYFCARGNNYYIYTDGGYAYAGLELWGPGILVTVSS SEQ ID NO. 109: 435 QSLEESGGDLVQPGASLTLTCKVSGFSLNTNYWMCWVRQAPGKG LEWIGCMYTGSYN RAYYASW 30 AKGRFTSSKTSSTTVTLEMTSLTAADTATYFCAKGSNWYSDLWGPGTLVTVSS SEQ ID NO, 110: 453 QERLVESGGGLVQPEGSLTLTCKASGFSFSRSYYIYWVRQAPGKGLE WIACIDAGSSGILVYANWAK GRFTISKTSSTTVTLQMTSLTAADTATYFCARGDASYGVDSFMLPLWGPGTLVTVSS 35 SEQ ID NO. 111: 375 QSLEESGGGLVQPEGSLTLTCKASG FSFTTTDYMCWVRQAPG KGLEWIGCILAG DGSTYYANWAK GR FTGSKTSSTTVDLKMTGLTAADTATYFCARSDPASSWSFALWGPGTLVTVSS 40 SEQ ID NO. 112: 610 QSLE ESGGRLVTPGTPLTLTCTASGIDFSGAYYMGWVRQAPGKGLEWIGYIDYDGDRYYASWAKG RFTISKTSTTVDLKITSPTTEDTATYFCA RSDYSSGWGTDIWGPGTLVTVSL SEQ ID NO. 113: 578 WO 2009/155724 PCT/CH2009/000220 86 QSVEESGGRLVTPGTPLTLTCTASGFSLTDYYYMTWVRLAPGKGLEYIGFIDPDDDPYYATWAKGRF TISRTSTTVNLKMTSPTTEDTATYFCAGGDHNSGWGLDIWGPGTLVTVSL SEQ ID NO. 114: 534 5 QSLEESGGRLVTPGTPLTLTCTASGFSLSYYYMSWVRQAPGKGLEWIGIIGPGDYTDYASWAKGRFT ISKTSTTVDLKITSPTTEDTATYFCGRGDDNSGWGEDIWGPGTLVTVSL SEQ ID NO. 115: 567 QSVEESGGRLVTPGAPLTLTCSVSGFSLSDYYMCWVRQAPGKGLQWIGCLDYFGSTDDASWAKGR 10 FTISKTSTAVDLKITSPTTEDTATYFCARTDDSRGWGLNIWGPGTLVTVSL SEQ ID NO. 116: 509 QSLEESGGRLVTPGTPLTLTCTASG FSLSSYYMCWVRQA PGKG LEWIGCLDYVG DTDYASWAKG RF TISKASTTVDLKITSLTTEDTATYFCARTDDSRGWG LNIWGPGTLVTVSL 15 SEQ ID NO. 117: 511 QSVEESGGRLVTPGTPLTLTCTVSGFSLNTYYMNWVRdAPGKGLEWIGIIAPDDTTYYASWAKSRST ITRDTN ENTVTLKMTSLTTEDTATYFCARSGDTTAWGADIWGPGTLVTVSL 20 SEQ ID NO. 118: 60-11-4min EVQLVESGGGLVQPGGSLRLSCAASGFPFSSGYWVCWVRQAPGKG LEWVSCIYAGSSGSTYYASW AKGRFTISRDNSKNTLYLQM NSLRAE DTAVYYCAKG N NYYIYTDGGYAYAG LELWGQGTLVTVSS SEQ ID NO. 119: 60-11-6min 25 EVQLVESGGGLVQPGGSLRLSCAASGFSFSSGYWICWVRQAPGKGLEWVSCIYAGSSGSTYYASW AKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGNNYYIYTDGGYAYAGLELWGQGTLVTVSS SEQ ID NO. 120: 435min EVQLVESGGGLVQPGGSLRLSCAASGFSLNTNYWMCWVRQAPGKGLEWVSCMYTGSYNRAYYA 30 SWAKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGSNWYSDLWGQGTLVTVSS SEQ ID NO. 121: 453min EVQLVESGGGLVQPGGSLRLSCAASGFSFSRSYYIYWVRQA PG KGLEWVSCIDAGSSGILVYANWA KGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGDASYGVDSFMLPLWGQGTLVTVSS 35 SEQ ID NO. 122: 375min EVQLVESGGG LVQPGGSLRLSCAASG FSFTTTDYMCWVRQAPGKGLEWVSCILAGDGSTYYANW AKGR FTISR DNSKNTLYLQMNSLRAEDTAVYYCAKSDPASSWSFALWGQGTLVTVSS 40 SEQ ID NO. 123: 610min EVQLVESGGGLVQPGGSLRLSCAASGIDFSGAYYMGWVRQAPGKGLEWVSYIDYDGDRYYASWA KGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKSDYSSGWGTDIWGQGTLVTVSS SEQ ID NO. 124: 578min WO 2009/155724 PCT/CH2009/000220 87 EVQLVESGGGLVQPGGSLRLSCAASGFSLTDYYYMTWVRQAPGKGLEWVSFIDPDDDPYYATWAK GRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGDH NSGWGLDIWGQGTLVTVSS SEQ ID NO. 125: 534min 5 EVQLVESGGGLVQPGGSLRLSCAASGFSLSYYYMSWVRQAPGKGLEWVSIIGPGDYTDYASWAKG RFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKGDDNSGWGEDIWGQGTLVTVSS SEQ ID NO. 126: 567min EVQLVESGGGL.VQPGGSLRLSCAASGFSLSDYYMCWVRQAPGKGLEWVSCLDYFGSTDDASWAK 10 GRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKTDDSRGWGLNIWGQGTLVTVSS SEQ ID NO. 127: 509min EVQLVESGGGLVQPGGSLRL.SCAASGFSLSSYYMCWVRQAPGKGLEWVSCLDYVG DTDYASWAK G RFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKTDDSRGWGLNIWGQGTLVTVSS 15 SEQ ID NO. 128: 511min EVQLVESGGGLVQPGGSLRLSCAASGFSLNTYYMNWVRQAPGKGLEWVSIIAPDDTTYYASWAKS R FTISRDNSKNTLYLQMNSLRAEDTAVYYCAKSGDTTAWGADIWGQGTLVTVSS 20 SEQ ID NO. 129: 578min Pref subst substQVQLVQTGGGLVQPGGSLRLSCAASGFSLTDYYYMTWVRQAPGKGLEWVSFIDPDDDPYY ATWAKGRFTISRDNSKNTVYLQMNSLRAEDTALYYCAKGDH NSGWGLDIWGQGTLVTVSS SEQ ID NO. 130: 60-11-4max 25 EVQLVESGGGLVQPGGSLRLSCTASGFPFSSGYWVCWVRQAPGKGLEWVGCIYAGSSGSTYYASW AKGRFTISKDTSKNTVYLQMNSLRAEDTAVYYCARGNNYYIYTDGGYAYAGLELWGQGTLVTVSS SEQ ID NO. 131: 60-11-6max EVQLVESGGGLVQPGGSLRLSCTASGFSFSSGYWICWVRQAPGKGLEWVGCIYAGSSGSTYYASW 30 AKGRFTISKDTSKNTVYLQMNSLRAEDTAVYYCARGNNYYIYTDGGYAYAGLELWGQGTLVTVSS SEQ ID NO. 132: 435max EVQLVESGGGLVQPGGSLRLSCKVSGFSLNTNYWMCWVRQAPGKGLEWVGCMYTGSYN RAYYA SWA KGRFTSSKDTSKNTVYLQMNSLRAEDTAVYYCAKGSNWYSDLWGQGTLVTVSS 35 SEQ ID NO. 133: 453max EVQLVESGGGLVQPGGSLRLSCKASGFSFSRSYYIYWVRQAPGKGLEWVGCIDAGSSGILVYANWA KGRFTISKDTSKNTVYLQMNSLRAEDTAVYYCARGDASYGVDSFMLPLWGQGTLVTVSS 40 SEQ ID NO. 134: 375max EVQLVESGGG LVQPGGSLRLSCKASG FSFTTTDYMCWVRQAPG KGLEWVGCI LAG DGSTYYANW AKGRFTGSKDTSKNTVYLQMNSLRAEDTAVYYCARSDPASSWSFALWGQGTLVTVSS SEQ ID NO. 135: 610max WO 2009/155724 PCT/CH2009/000220 88 EVQLVESGGGLVQPGGSLR LSCTASGIDFSGAYYMGWVRQA PGKGLEWVGYIDYDGDRYYASWA KG RFTISKDTSKNTVYLQMNSLRAEDTAVYYCARSDYSSGWGTDIWGQGTLVTVSS SEQ ID NO. 136: 578max 5 EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGL DIWGQGTLVTVSS SEQ ID NO. 137: 534max EVQLVESGGGLVQPGGSLRLSCTASGFSLSYYYMSWVRQAPGKGLEWVGIIGPGDYTDYASWAKG 10 RFTISKDTSKNTVYLQMNSLRAEDTAVYYCARGDDNSGWGEDIWGQGTLVTVSS SEQ ID NO. 138: 567max EVQLVESGGGLVQPGGSLRLSCSVSGFSLSDYYMCWVRQAPGKGLEWVGCLDYFGSTDDASWAK GRFTISKDTSKNTVYLQMNSLRAEDTAVYYCARTDDSRGWGLNIWGQGTLVTVSS 15 SEQ ID NO. 139: 509max EVQLVESGGGLVQPGGSLRLSCTASGFSLSSYYMCWVRQAPGKGLEWVGCLDYVGDTDYASWAK GRFTISKDASKNTVYLQMNSLRAEDTAVYYCARTDDSRGWGLNIWGQGTLVTVSS 20 SEQ ID NO. 140: 509maxIl EVQLVESGGGLVQPGGSLRLSCTASGFSLSSYYMSWVR QAPGKGLEWVGILDYVGDTDYASWAKG RFTISKDASKNTVYLQMNSLRAEDTAVYYCARTDDSRGWGLNIWGQGTLVTVSS SEQ ID NO. 141: 511max 25 EVQLVESGGGLVQPGGSLRLSCTVSGFSLNTYYMNWVRQAPGKGLEWVGIIAPDDTTYYASWAKS RSTISRDTSKNTVYLQMNSLRAEDTAVYYCARSGDTTAWGADIWGQGTLVTVSS SEQ ID NO. 142: 578maxPref subst substQVQLVQTGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYY 30 ATWAKGRFTISRDTSKNTVYLQM NSLRAEDTALYYCAGGDH NSGWGLDIWGQGTLVTVSS SEQ ID NO. 143: 567minDHP EVQLVESGGGSVQPGGSLRLSCAASGFSLSDYYMCWVRQAPGKGLEWVSCLDYFGSTDDASWAK GRFTISRDNSKNTLYLQMNSLRAEDTATYYCAKTDDSRGWGLNIWGQGTTVTVSS 35 SEQ ID NO. 144: 578maxDHP EVQLVESGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDTSKNTVYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTTVTVSS 40 SEQ ID NO. 145: 511maxDHP EVQLVESGGGSVQPGGSLRLSCTVSGFSLNTYYMNWVRQAPGKGLEWVGIIAPDDTTYYASWAKS RSTISRDTSKNTVYLQMNSLRAEDTATYYCARSGDTTAWGADIWGQGTTVTVSS SEQ ID NO. 146: 578maxPref substDHP WO 2009/155724 PCT/CH2009/000220 89 QVQLVQTGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDTSKNTVYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTTVTVSS SEQ ID NO. 147: 578max VH: El 5 VQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWAK GRFTISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDH NSGWGLDIWGQGTLVTVSS SEQ ID NO. 148: 578max VH: V2Q EQQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA 10 KGRFTISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 149: 578max VH: Q46L EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRLAPGKGLEWVGFIDPDDDPYYATWAK GRFTISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS 15 SEQ ID NO. 150: 578max VH: W54Y EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEYVGFIDPDDDPYYATWAK GRFTISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS 20 SEQ ID NO. 151: 578max VH: V551 EVQLVESGGG LVQPGGSLR LSCTASGFSLTDYYYMTWVRQAPG KG LEWIGFI DPDDDPYYATWAK GRFTISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 152: 578max VH: D83A 25 EVQLVESGGGLVQPGGSLR LSCTASGFSLTDYYYMTWVRQAPGKG LEWVGFIDPDDDPYYATWA KGRFTISRATSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 153: 578max VH: N87A EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDD PYYATWA 30 KGRFTISRDTSKATVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 154: 578max VH: Y105F EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMIWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDTSKNTVYLQMNSLRAEDTAVYFCAGGDHNSGWGLDIWGQGTLVTVSS 35 SEQ ID NO. 155: 578max VH: D83_ EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDD PYYATWA KGRFTISRTSKNTVYLQMNSLRAE DTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS 40 SEQ ID NO. 156: 578max VH: N87 EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDTSKTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO, 157: 578max VH: T84N WO 2009/155724 PCT/CH2009/000220 90 EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDNSKNTVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 158: 578max VH: V89L 5 EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDTSKNTLYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 159: 578max VH: V89A EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA 10 KGRFTISRDTSKNTAYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 160: 578maxDHP VH: T84N EVQLVESGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDNSKNTVYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTIVTVSS 15 SEQ ID NO. 161: 578maxDHP VH: V89L EVQLVESGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPUODPYYATWA KGRFTISRDTSKNTLYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTTVTVSS 20 SEQ ID NO. 162: 578maxDHP VH: V89A EVQLVESGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGILEWVGFIDPDDDPYYATWA KGRFTISRDTSKNTAYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTTVTVSS SEQ ID NO. 163: 578max VH:T84N, V89A 25 EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTlWVRQAPGKGLEWVGFIDPDDDPYYATWA KGRFTISRDNSKNTAYLQMNSLRAE DTAVYYCAGGDH NSGWGLDIWGQGTLVTVSS SEQ ID NO. 164: 578max VH:T84N, V89L EVQLVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWA 30 KGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS SEQ ID NO. 165: 578maxDHP VH: T84N, V89A EVQLVESGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQA PGKGLEWVGFIDPDDDPYYATWA KGRFTISRDNSKNTAYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTTVTVSS 35 SEQ ID NO. 166: 578maxDHP VH: T84N, V89L EVQLVESGGGSVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKG LEWVGFIDPDDDPYYATWA KGRFTISRDNSKNTLYLQMNSLRAEDTATYYCAGGDHNSGWGLDIWGQGTTVTVSS 40 framework sequences (X residues are CDR insertion sites and may be any naturally occurring amino acid. At least 3 and up to SO amino acids can be present): SEQ ID NO. 167: Variable light chain FWI.4 and rFW1.4 WO 2009/155724 PCT/CH2009/000220 91 EIVMTQSPSTLSASVGDRVIITC(X)n=3-so WYQQKPGKAPKLLIY(X)n=3- 50 GVPSRFSGSGSGAEFTLTISSLQPDDFATYYC(X)n=3-so FGQGTKLT VLG SEQ ID NO. 168: Variable light chain rFW1.4 variant 2 (v2) 5 EIVMTQSPSTLSASVGDRVIITC(X)n=3- 5 o WYQQKPGKAPKLLIY(X)n=3-so GVPSRFSGSGSGAEFTLTISSLQPDDFATYYC(X)n=3-so FGQGTKLT VLG SEQ ID NO. 169: Variable heavy chain FWI.4 EVQLVESGGGLVQPGGSLRLSCAAS(X)n=3-so WVRQAPGKGLEWVS (X)n=3- 50 10 R FTISRDNSKNTLYLQM NSLRAEDTAVYYCAK(X)n=3- 50 WGQGTL VTVSS SEQ ID NO. 170: Variable heavy chain rFW1.4 EVQLVESGGGLVQPGGSLRLSCTAS(X)n=3- 50 WVRQAPGKGLEWVG(X)n=3- 50 RFTISRDTSKNTVYLQMNSLRAEDTAVYYCAR(X)n=3-so WGQGTLV TVS 15 SEQ ID NO. 171: Variable heavy chain rFWI.4 variant 2 (v2) EVQLVESGGGLVQPGGSLRLSCTVS(X)n=3- 50 WVRQAPGKGLEWVG(X)n=3- 50 RFTISKDTSKNTVYLQMNSLRAEDTAVYYCAR(X)n=3-so WGQGTLVTVSS 20 ScFv framework sequences: SEQ ID NO. 172: FWI.4 EIVMTQSPSTLSASVGDRVIITC(X)n=3-50 WYQQKPGKAPKLLlY(X)n-so 25 GVPSRFSGSGSGAEFTLTISSLQPDDFATYYC(X)n=3- 5 o FGQGTKLTVLG GGGGSGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCAAS(X)n=3- 50 WVRQAPGKGLEWVS(X)n=3-so RFTISRDNSKNTLYLQMNSLRAEDTA

VYYCAK(X)

5 =3-s 0 WGQGTLVTVSS 30 SEQ ID NO. 173: rFWI.4 EIVMTQSPSTLSASVGDRVIITC(X)n=3- 5 WYQQKPGKAPKLLIY(X)n=3-so GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC(X)=3.so FGQGTKLTVLG GGGGSGGGGSGGGGSGGGGSEVQLVESGGGLVQPGGSLRLSCTAS(X)n=3-so WVRQAPGKGLEWVG(X)n=3.sO RFTISRDTSKNTVYLQMNS LRAEDTAVYYCAR(X)n=3-s 5 35 WGQGTLVTVSS SEQ ID NO, 174: rFW1.4 variant 2 (v2) EIVMTQSPSTLSASVGDRVIITC(X)n=3-so WYQQKPGKAPKLLIY(X)n=3-o 5 0 GVPSRFSGSGSGTEFTLTISSLQPDDFATYYC(X).=3-s 0 FGQGTKLTVLG 40 GGGGSGGGGSGGGGSGGGGS EVQLVESGGGLVQPGGSLRLSCTVS(X)n=3- 50 WVRQAPGKGLEWVG(X)n=3-so RFTISKDTSKNTVYLQMNSLR AEDTAVYYCAR(X)n=3-s 0

WGQGTLVTVSS

WO 2009/155724 PCT/CH2009/000220 92 ScFv anti-VEGF sequences: SEQ ID NO. 175: 435_max 5 EIVMTQSPSTLSASVGDRVIITCQASQSIGSSLAWYQQKPGKAPKLLYTAANLASGVPSRFSGSRSG AEFTLTISSLQPDDFATYYCQNFATSDTVTFGQGTKLVLGGGGGSGGGGSGGGGSGGGGSEVQL VESGGGLVQPGGSLRLSCKASGFSLNTNYWMCWVRQAPGKGLEWVGCMYTGSYNRAYYASWA KG R FTSSKDTSKNTVYLQMNSLRAEDTAVYYCAKGSNWYSDLWGQGTLVTVSS 10 SEQ ID NO. 176: 511_max EIVMTQSPSTLSASVGDRVIITCQASQSINIWLSWYQQKPGKAPKLLIYRASTLASGVPSRFSGSGSGT EFTLTISSLQPDDFATYYCQANYAYSAGYGAAFGQGTKLWLGGGGGSGGGGSGGGGSGGGGSEV QLVESGGGLVQPGGSLRLSCTVSGFSLNTYYMNWVRQAPGKGLEWVGIIAPDDTTYYASWAKSRS TISRDTSKNTVYLQMNSLRAEDTAVYYCARSGDTTAWGADIWGQGTLVTVSS 15 SEQ ID NO. 177: 567_min EIVMTQSPSTLSASVGDRVIITCQADQSIYIWLSWYQQKPGKAPKLLIYKASTLESGVPSRFSGSGSGA EFTLTISSLQPDDFATYYCQNNAHYSTNGGTFGQGTKLTVLGGGGGSGGGGSGGGGSGGGGSEVQ LVESGGGLVQPGGSLRLSCAASGFSLSDYYMCWVRQAPGKGLEWVSCLDYFGSTDDASWAKGRFT 20 ISRDNSKNTLYLQMNSLRAEDTAVYYCAKTDDSRGWGLNIWGQGTLVTVSS SEQ ID NO. 178: 578min EIVMTQSPSTLSASVGDRVIITCQASEIIHSWLAWYQQKPGKAPKLLIYLASTLASGVPSRFSGSGSGA 25 EFTLTISSLQPDDFATYYCQNVYIASTNGANFGQGTKLTVLGGGGGSGGGGSGGGGSGGGGSEVQ LVESGGGLVQPGGSLRLSCAASGFSLTDYYYMTWVRQAPGKGLEWVSFIDPDDDPYYATWAKGRF TISR DNSKNTLYLQMNSLRAEDTAVYYCAKGD HNSGWGLDIWGQGTLVTVSS SEQ ID NO. 179: 578max 30 EIVMTQSPSTLSASVGDRVIITCQASEIIHSWLAWYQQKPGKAPKLLIYLASTLASGVPSRFSGSGSGT QFTLTISSLQPDDFATYYCQNVYLASTNGANFGQGTKLTVLGGGGGSGGGGSGGGGSGGGGSEVQ LVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLEWVGFIDPDDDPYYATWAKGRF TISRDTSKNVYLQMNSLRAEDTAVYYCAGGDHNSGWGLDIWGQGTLVTVSS 35 SEQ ID NO. 180: 578minmax (ESBA903) EIVMTQSPSTLSASVGDRVIITCQASEIHSWLAWYQQKPGKAPKLLIYLASTLASGVPSRFSGSGSGA EFTLTISSLQPDDFATYYCQNVYLASTNGANFGQGTKLTVLGGGGGSGGGGSGGGGSGGGGSEVQ LVESGGGLVQPGGSLRLSCTASGFSLTDYYYMTWVRQAPGKGLE WVGFIDPDDDPYYATWAKGRF TISRDTSKNTVYLQMNSLRAEDTAVYYCAGGDH NSGWGLDIWGQGTLVTVSS 40 SEQ ID No. 181 linker

GGGGSGGGGSGGGGSGGGGS

Claims

1. A recombinant antibody comprising a variable heavy chain (VH) and a variable light chain (VL), wherein the heavy chain variable region comprises the amino acid sequence of SEQ ID NO: 164 and the light chain variable region comprises the amino acid sequence of SEQ ID NO: 87.

2. An antigen-binding fragment of the recombinant antibody of claim 1, wherein the fragment is a Fab, a F(ab') 2 or a Fab'.

3. An antigen-binding fragment of the recombinant antibody of claim 1, wherein the fragment is an scFv.

4. The antigen-binding fragment of claim 3, wherein the heavy chain variable region and the light chain variable region are linked by the sequence of SEQ ID NO: 181.

5. A pharmaceutical composition comprising the recombinant antibody of claim 1.

6. A pharmaceutical composition comprising the antigen-binding fragment of claim 2 or 3.

7. A pharmaceutical composition comprising the antigen-binding fragment of claim 4.

8. The pharmaceutical composition of any one of claims 5 to 7, formulated for topical, intraocular, oral, nasal, rectal or parenteral administration.

9. An isolated nucleic acid molecule encoding the antibody of claim 1 or the antigen binding fragment of any one of claims 2, 3 or 4.

10. An expression vector comprising the nucleic acid molecule of claim 9.

11. A host cell comprising the expression vector of claim 10. 94

12. Use of the antibody of claim 1 or the antigen-binding fragment of claims 2, 3 or 4 in the production of a medicament for the treatment or prevention of a human VEGF-mediated disease.

13. A method of treating a human VEGF-mediated disease in a subject, comprising administering to a subject a therapeutically effective amount of the antibody of claim 1, such that the subject is treated for a VEGF-mediated disease.

14. The use of claim 12 or the method of claim 13, wherein the VEGF-mediated disease is selected from the group consisting of age-related macular degeneration, neovascular glaucoma, diabetic retinopathy, retinopathy of prematurity, retrolental fibroplasia, breast carcinomas, lung carcinomas, gastric carcinomas, esophageal carcinomas, colorectal carcinomas, liver carcinomas, ovarian carcinomas, the comas, arrhenoblastomas, cervical carcinomas, endometrial carcinoma, endometrial hyperplasia, endometriosis, fibrosarcomas, choriocarcinoma, head and neck cancer, nasopharyngeal carcinoma, laryngeal carcinomas, hepatoblastoma, Kaposi's sarcoma, melanoma, skin carcinomas, hemangioma, cavernous hemangioma, hemangioblastoma, pancreas carcinomas, retinoblastoma, astrocytoma, glioblastoma, Schwannoma, oligodendroglioma, medulloblastoma, neuroblastomas, rhabdomyosarcoma, osteogenic sarcoma, leiomyosarcomas, urinary tract carcinomas, thyroid carcinomas, Wilm's tumor, renal cell carcinoma, prostate carcinoma, abnormal vascular proliferation associated with phakomatoses, edema (such as that associated with brain tumors), Meigs' syndrome, rheumatoid arthritis, psoriasis and atherosclerosis.

15. A method of treating a human VEGF-mediated disease in a subject, comprising administering to a subject a therapeutically effective amount of an antigen-binding fragment of claims 2, 3 or 4, such that the subject is treated for a VEGF-mediated disease.

16. The method of claim 15, wherein the VEGF-mediated disease is selected from the group consisting of age-related macular degeneration, neovascular glaucoma, diabetic retinopathy, retinopathy of prematurity, retrolental fibroplasia, breast carcinomas, lung 95 carcinomas, gastric carcinomas, esophageal carcinomas, colorectal carcinomas, liver carcinomas, ovarian carcinomas, the comas, arrhenoblastomas, cervical carcinomas, endometrial carcinoma, endometrial hyperplasia, endometriosis, fibrosarcomas, choriocarcinoma, head and neck cancer, nasopharyngeal carcinoma, laryngeal carcinomas, hepatoblastoma, Kaposi's sarcoma, melanoma, skin carcinomas, hemangioma, cavernous hemangioma, hemangioblastoma, pancreas carcinomas, retinoblastoma, astrocytoma, glioblastoma, Schwannoma, oligodendroglioma, medulloblastoma, neuroblastomas, rhabdomyosarcoma, osteogenic sarcoma, leiomyosarcomas, urinary tract carcinomas, thyroid carcinomas, Wilm's tumor, renal cell carcinoma, prostate carcinoma, abnormal vascular proliferation associated with phakomatoses, edema (such as that associated with brain tumors), Meigs' syndrome, rheumatoid arthritis, psoriasis and atherosclerosis.

17. A bispecific molecule comprising the antibody of claims 1 or the antigen-binding fragment of claims 2, 3 and 4.

18. The bispecific molecule of claim 17, being multispecific.

19. The recombinant antibody according to claim 1, substantially as hereinbefore described with reference to any of the Examples and/or Figures.