AU2013202948B2 - A process for concentration of a polypeptide - Google Patents

Abstract

The present invention comprises a method of concentrating a composition comprising a polypeptide of interest and the use of such a concentrated composition for the treatment of diseases in mammals, in particular by subcutaneous injection.

Description

1 AUSTRALIA Patents Act 1990 ZYMENEX A/S COMPLETE SPECIFICATION STANDARD PATENT Invention Title: A process for concentration of a polypeptide The following statement is a full description of this invention including the best method of performing it known to us:- A PROCESS FOR CONCENTRATION OF A POLYPEPTIDE FIELD OF THE INVENTION j' The present invention relates to a method for concentrating a polypeptide of interest, to the use of a composition comprising a concentrated polypeptide of interest as a medicament for subcutaneous injection and to a composition comprising at least 10 mg/mI polypeptide of interest, 10 BACKGROUND OF THE INVENTION Some polypeptides are useful as a medicament for the prevention and/or treatment of certain diseases. The ability to inject a medicament subcutaneously is an advantage as it makes it easy for the patients to administer the medication to themselves. 15 As there are physiological restrains on how large a volume it is possible to inject subcutaneously, Thus it is an advantage for medicaments which are to be administered subcutaneously that they are available in a high concentration so as to ensure that the patient recieves an adequate amount of the medicament and/or to avoid multiple subcutaneous injections, 20 WO 99/37325 discloses methods of treating and preventing disease caused by absence or deficiency of the activity of enzymes belonging to the heme biosynthetic pathway. WO 03/002731 discloses a process for purification of recombinant porphobilinogen deaminase on an industrial scale and to the use of the purified product for the preparation of a medicament, Similarly, WO 25 02/099092 and WO 2005/094874 provides lysosomal alpha-mannosidase and therapeutic use hereof. Finally, WO 2005/073367 provides a process for purification of aryl sulfatase A and use of the enzyme in the treatment of metachromatic leukodystrophy. The present invention relates to a method for concentrating a polypeptide of 30 interest and to the use of a compositon comprising a concentrated polypeptide of interest for the manufacture of a medicament for subcutaneous injection into mam mal .

Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is not to be taken as an admission that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present disclosure as it 5 existed before the priority date of each claim of this application. Throughout this specification the word comprisedn or variations such as "comprises" or comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or If steps.

SUMMARY OF THE INVENTION In a first aspect the invention provides a method of concentrating a composition comprising porphobilinogen dearninase and/or a functionally equivalent part and analogue hereof, said method comprisinq: 5 a) centrifugation followed by filtration of a composition comprising said porphobilinogen deaminase; b) concentrating the retentate, obtained from step a); an d c) obtaining from the concentrated retenate of step b) an isotonic solution comprising porphobilinogen deaminase, I0 wherein the amount of porphobilinogen deaminase present as aggregates is less than 5 w/w% of the total amount of porphobilinogen deaminase, and wherein the concentration is at least 50 mg/mI of porphobilinogen deaminase and functionally equivalent parts and analogues hereof, 17 In a second aspect the invention provides a composition comprising at least 75 mg/mi of porphobilinogen deaminase and functionally equivalent parts and analogues hereof, wherein less than 5% of the total amount of the porphobilinogen deaminase in said composition is present in the form of aggregates, 20 In a third aspect the invention provides use of a composition comprising 50-300 mg/mI of porphobilinogen deaminase and functionally equivalent parts and analogues hereof in the manufacture of a medicament for subcutaneous injection into a mammal In a fourth aspect the invention provides a method of treating a mammal for 25 acute intermittent porphyria comprising subcutaneous injection of a composition of 50-300 mg/ni porphobilinogen deaminase. DEFINTIONS For purposes of the present invention, alignments of sequences and calculation of homology scores may be done using a full Smith-Waterman alignment, useful for 30 both protein and DNA alignments. The default scoring matrices BLOSUMS0 and the identity matrix are used for protein and DNA alignments respectively. The penalty for the first residue in a gap is - 12 for proteins and -16 for DNA. while the penalty for additional residues in a gap is -2 for proteins and -4 for DNA, 4 Alignment may be made with the FASTA package version v20u6 (W. R. Pearson and D. J. Lipman (1988), "Improved Tools for Biological Sequence Analysis", PNAS 85:2444-2448, and W. R. Pearson (1990) "Rapid and Sensitive Sequence Comparison with FASTP and FASTA", Methods in Enzymology, 183:63-98). 5 Multiple alignments of protein sequences may be made using "ClustalW" (Thompson, J. D., Higgins, D. G. and Gibson, TJ. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680). Multiple alignment of DNA sequences may be done 10 using the protein alignment as a template, replacing the amino acids with the corresponding codon from the DNA sequence. In the context of the present invention, the term "E. C." (Enzyme Class) refers to the internationally recognized enzyme classification system, Recommendations of the Nomenclature Committee of the International Union of Biochemistry and 15 Molecular Biology, Academic Press, Inc. The term "origin" used in the context of amino acid sequences, e.g. proteins, or nucleic acid sequences is to be understood as referring to the organism from which it derives. Said sequence may be expressed by another organism using gene technology methods well known to a person skilled in the art. This also 20 encompasses sequences which have been chemically synthesized. Furthermore, said sequences may comprise minor changes such as codon optimization, i.e. changes in the nucleic acid sequences which do not affect the amino acid sequence. 25 DETAILED DESCRIPTION OF THE INVENTION Polypeptide of interest The polypeptide of the present invention may in particular be a hormone or hormone variant, an enzyme, a receptor or portion thereof, an antibody or portion thereof, an allergen or a reporter. The polypeptide of interest may in particular be 30 an enzyme seleted from one of six major enzyme groups, such as an oxidoreductase (E.C. 1), a transferase (E.C. 2), a hydrolase (E.C. 3), a lyase (E.C. 4), an isomerase (E.C. 5), or a ligase (E.C. 6). In a more particular aspect, the polypeptide of interest may be an aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, cellobiohydrolase, chitinase, cutinase, 35 cyclodextrin glycosyltransferase, deoxyribonuclease, endoglucanase, esterase, 5 alpha-galactosidase, beta-galactosidase, glucoamylase, alpha-glucosidase, beta glucosidase, invertase, laccase, lipase, mannosidase, mutanase, oxidase, pectinolytic enzyme, peroxidase, phospholipase, phytase, polyphenoloxidase, proteolytic enzyme, ribonuclease, transglutaminase, xylanase, or beta-xylosidase. 5 The polypeptide of interest may in particular be a polypeptide which is useful as a medicament. Examples of a suitable polypeptide of interest include but is not limited to one selected from the group consisting of a phorphobilinogen deaminase, an aryl sulfatase, an alpha-mannosidase and a galactocerebrosidase. 10 In principle a polypeptide of interest derivable from any source may be treated according to the methods of the present invention. In a particular embodiment the polypeptide of interest may be of human origin. Especially in the context of using a polypeptide of interest for the manufacture of a medicament which is to be administered to humans may the polypeptide be of 15 human origin as this may minimize the risk of unwanted allergic reactions. Natural variations of human polypeptide due to e.g. polymorphism are in the context of the present invention included in the term "human origin". The polypeptide of interest may in particular be produced as a recombinant protein, i.e. a nucleotide sequence encoding the polypeptide of interest may be 20 introduced into a cell for expression of the polypeptide of interest. The recombinant expression may be homologous or heterologous, i.e. the polypeptide of interest may be expressed in cell which it is naturally expressed by (homologous expression) or it may be expressed by a cell which it is not naturally expressed by (heterologous expression). 25 The recombinant polypeptide of interest may be expressed by any cell suitable for recombinant production of the particular polypeptide of interest. Examples of suitable cells include but are not limited to prokaryotic cells, such as an E.coli cell or a Bacillus cell. Examples of suitable eukaryotic cells include but are not limited to a yeast cell or a mammalian cell such as a Chinese Hamster Ovary (CHO). 30 Alternatively, it may be a human cell. Suitable host cells for the expression of glycosylated polypeptide are derived from multicellular organisms. Examples of invertebrate cells include plant and insect cells. However, the host cell may also be a vertebrate cell, and propagation of vertebrate cells in culture (tissue culture) has become a routine procedure 6 The term "recombinant polypeptide" or "recombinant polypeptide of interest" denotes herein a recombinant produced polypeptide. Reference to a particular polypeptide of interest includes in the context of the present invention also functionally equivalent parts or analogues of the 5 polypeptide of interest. For example, if the polypeptide of interest is an enzyme a functionally equivalent part of the enzyme could be a domain or subsequence of the enzyme which includes the necessary catalytic site to enable the domain or subsequence to exert substantially the same enzymatic activity as the full-length enzyme or alternatively a gene coding for the catalyst. The term "substantially the 10 same enzymatic activity" refers to an equivalent part or analogue having at least 50%, preferably at least 60%, more preferably at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95% and most preferably at least 97%, at least 98% or at least 99% of the activity of the natural enzyme. An 15 example of an enzymatically equivalent analogue of the enzyme could be a fusion protein which includes the catalytic site of the enzyme in a functional form, but it can also be a homologous variant of the enzyme derived from another species. Also, completely synthetic molecules that mimic the specific enzymatic activity of the relevant enzyme would also constitute "enzymatic equivalent analogues". 20 Generally, the skilled person will be able to readily devise appropriate assays for the determination of enzymatic acitivity. For PBGD, however, a suitable assay is described in WO 03/002731, in example 2, as well as in the experimental sections of the present applications. Aryl sulfhatase, in addition to its natural substrates, is also able to catalyze the hydrolysis of the synthetic, chromogenic substrate, para 25 Nitrocatechol sulfate (pNCS). The product, para-Nitrocatechol (pNC), absorbs light at 515 nm. An assay for determination of aryl sulfatase activity is described in details in WO 2005/073367 and in Fluharty et al. 1978, Meth. Enzymol. 50:537 47. For LAMAN, an appropriate enzyme activity assay is disclosed in WO 02/099092. 30 Porphobilinoqen deaminase In one embodiment the polypeptide of interest of the invention may be porphobilinogen deaminase, (also known as porphobilinogen ammonia-lyase (polymerizing)), E.C. 4.3.1.8. (Waldenstr6m 1937, J. Acta.Med. Scand. Suppl.8). 35 Porphobilinogen deaminase is the third enzyme in the heme biosynthetic pathway. E.C. 4.3.1.8 has been transferred to E.C. 2.5.1.61, so porphobilinogen deaminase (PBGD) is now placed under this E.C. number.

7 Porphobilinogen deaminase catalyzes the reaction of 4 porphobilinogen + H 2 0 = hydroxymethylbilane + 4 NH 3 . PBDG is important in relation to Acute intermittent porphyria (AIP), which is an autosomal dominant disorder in man caused by a defect (50% reduction of 5 activity) of PBDG (see W001/07065 for further details in relation to this). Porphobilinogen deaminase is in short known as PBGD and in the context of the present invention these two terms may be used inter-changeably with one another. For recombinant expression of PBGD a host cell may in particular be a yeast cell 10 or an E.coli cell. For a detailed example of construction of a recombinant E.coli cell reference is made to example 1 of W001/07065 and for construction of recombinant HeLa cells and NIH 3T3 cells capable of expressing mouse PBGD reference is made to example 6 of WO01/07065. 15 The term "recombinant porphobilinogen deaminase (rPBGD)" denotes herein a recombinant produced PBGD. In the following, this enzyme and the recombinant human form will be termed "PBGD" and "rhPBGD", respectively. Within this term is also included an enzymatically equivalent part or analogue of PBGD. One example of an enzymatically equivalent part of the enzyme could be a domain or 20 subsequence of the enzyme which includes the necessary catalytic site to enable the domain or subsequence to exert substantially the same enzymatic activity as the full-length enzyme or alternatively a gene coding for the catalyst. The term "substantially the same enzymatic activity" refers to an equivalent part or analogues enzyme having at least 50%, preferably at least 60%, more preferably 25 at least 70%, more preferably at least 75%, more preferably at least 80%, more preferably at least 85%, more preferably at least 90%, more preferably at least 95% and most preferably at least 97%, at least 98% or at least 99% of the activity of natural human rhPBGD measured in the rhPBGD activity assay described in example 2 of WO 03/002731. An example of an enzymatically 30 equivalent analogue of the enzyme could be a fusion protein which includes the catalytic site of the enzyme in a functional form, but it can also be a homologous variant of the enzyme derived from another species. Also, completely synthetic molecules that mimic the specific enzymatic activity of the relevant enzyme would also constitute "enzymatic equivalent analogues".

8 An example of PBGD which may be used in the present invention includes any of those shown in Sequence 1-10 of the present application, or in Genebank no. X04217, X04808 or M95623. Aryl sulfatase 5 In another embodiment of the present invention the polypeptide of interest may be an arylsulfatase A. Arylsulfatase A catalyzes the reaction of a cerebroside 3-sulfate + H20 = a cerebroside + sulphate. ASA has been purified from a variety of sources including human liver, placenta, 10 and urine. It is an acidic glucoprotein with a low isoelectric point. Above pH 6.5, the enzyme exists as a dimer with a molecular weight of approximately 110 kDa. ASA undergoes a pH-dependent polymerisation forming an octamer at pH 4.5. In human urine, the enzyme consists of two nonidentical subunits of 63 and 54 kDa. ASA purified from human liver, placenta, and fibroblasts also consist of two 15 subunits of slightly different sizes varying between 55 and 64 kDa. As in the case of other lysosomal enzymes, ASA is synthesised on membrane-bound ribosomes as a glycosylated precursor. It then passes through the endoplasmic reticulum and Golgi, where its N-linked oligosaccharides are processed with the formation of phosphorylated and sulfated oligosaccharide of the complex type (Waheed A et al. 20 Biochim Biophys Acta. 1985, 847, 53-61, Braulke T et al. Biochem Biophys Res Commun. 1987, 143, 178-185). In normal cultured fibroblasts, a precursor polypeptide of 62 kDa is produced, which translocates via mannose-6-phosphate receptor binding (Braulke T et al. J Biol Chem. 1990, 265, 6650-6655) to an acidic prelysosomal endosome (Kelly BM et al. Eur J Cell Biol. 1989, 48, 71-78). 25 The arylsulfatase A may in particular be of human origin. The length (18 amino acids) of the human ASA signal peptide is based on the consensus sequence and a specific processing site for a signal sequence. Hence, from the deduced human ASA cDNA (EMBL GenBank accession numbers J04593 and X521151) the cleavage of the signal peptide should be done in all cells after residue number 18 (Ala), 30 resulting in the mature form of the human ASA. In the following, recombinant arylsulfatase A will be abbreviated rASA, the mature form of arylsulfatase A including the mature form of human ASA will be termed "mASA" and the mature recombinant human ASA will be termed "mrhASA". A protein modification has been identified in two eukaryotic sulfatases (ASA and 35 arylsulfatase B (ASB)) and for one from the green alga Volvox carteri (Schmidt B et al. Cell. 1995, 82, 271-278, Selmer T et al. Eur J Biochem. 1996, 238, 341- 9 345). This modification leads to the conversion of a cysteine residue, which is conserved among the known sulfatases, into a 2-amino-3-oxopropionic acid residue (Schmidt B et al. Cell. 1995, 82, 271-278). The novel amino acid derivative is also recognised as C*-formylglycin (FGly). In ASA and ASB derived 5 from MSD cells, the Cys-69 residue is retained. Consequently, it is proposed that the conversion of the Cys-69 to FGly-69 is required for generating catalytically active ASA and ASB, and that deficiency of this protein modification is the cause of MSD. Cys-69 is referred to the precursor ASA which has an 18 residue signal peptide. In the mASA the mentioned cysteine residue is Cys-51. Further 10 investigations have shown that a linear sequence of 16 residues surrounding the Cys-51 in the mASA is sufficient to direct the conversion and that the protein modification occurs after or at a late stage of co-translational protein translocation into the endoplasmic reticulum when the polypeptide is not yet folded to its native structure (Dierks T et al. Proc Natl Acad Sci. 1997, 94, 11963-1196, Wittke, D. et 15 al. (2004), Acta Neuropathol. (Berl.), 108, 261-271). Multiple forms of ASA have been demonstrated on electrophoresis and isoelectric focusing of enzyme preparations from human urine, leukocytes, platelets, cultured fibroblasts and liver. Treatment with endoglycosidase H, sialidase, and alkaline phosphatase reduces the molecular size and complexity of the electrophoretic 20 pattern, which suggests that much of the charge heterogeneity of ASA is due to variations in the carbohydrate content of the enzyme. The arylsulfatase A may in particular be a form of arylsulfatase A, which is capable of crossing the blood brain barrier and/or a form of rASA, which possesses specific tags for entry into target cells within the brain. In particular, it may be a rASA, 25 which is efficiently endocytosed in vivo via the mannose-6-phosphate pathway. Thus the ASA may in particular be covalently bound to a so-called tag, peptides or proteins as vehicles or toxins as vehicles which are capable of increasing and/or facilitating transport of ASA over the blood-brain barrier and/or across cellular membranes in general (Schwarze et al.,Trends Cell Biol. 2000; 10(7): 290-295; 30 Lindgren et al., Trends Pharmacol. Sci. 2000; 21(3): 99-103). An ASA molecule containing such peptide sequences can be produced by expression techniques. The protein transduction process is not cell type specific and the mechanism by which it occurs is not fully elucidated, however, it is believed that it takes place by some sort of membrane perturbation and penetration process that is receptor 35 independent. A partially unfolded state of the molecule may facilitate the process but is not essential. An example of a suitable tag includes but is not limited to the mannose-6 phosphate tag.

10 Examples of peptides or proteins as vehicle include but are not limited to so-called protein-transducing domains. Examples of suitable protein-transducing domains include but are not limited to those mentioned in WO 2005/073367, which is incorporated herein by reference. Hence the protein-transducing domain may be 5 the 11 residue basic peptide from the HIV TAT protein -YGRKKRRQRRR (Schwarze et al.,Trends Cell Biol. 2000; 10(7): 290-295), a synthetic version of TAT YARAAARQARA that confers more alpha-helicity and amphipathic nature to the sequence (Ho et al., Cancer Res. 2001; 61(2):474-477), a synthetic leader peptide composed of poly -R or a mixture of basic -R and -K residues in 10 combination with other amino acids and peptides based on hydrophobic signal sequence moieties from either beta-3 integrin or Kaposi's sarcoma FGF (Dunican et al. Biopolymers 2001; 60(1): 45-60). Examples of suitable toxins as vehicles include but are not limited to those described in WO 2005/073367, which is incorporated herein by reference. 15 The ASA may in particular comprise a nucleic acid sequence, which encodes: (a) the amino acid sequence of SEQ ID NO:2 in WO 2005/073367; (b) a portion of the sequence in (a), which is enzymatically equivalent to recombinant human arylsulfatase A (c) an amino acid sequence analogue having at least 75% sequence identity to 20 any one of the sequences in (a) or (b) and at the same time comprising an amino acid sequence, which is enzymatically equivalent to recombinant human arylsulfatase A. In the present context, an amino acid sequence or a portion of an amino acid sequence which is a polypeptide capable of hydrolysing an amount of the 25 arylsulfatase A substrate pNCS at 37 0 C a rate corresponding to a specific activity of at least 20 U/mg polypeptide (preferably 50 U/mg polypeptide) when determined in an assay for measuring arylsulfatase A activity as described in example 1 of WO 2005/073367, and/or a polypeptide, which is capable of hydrolysing at least 40% of labelled arylsulfatase A substrate, fx. 14C palmitoyl 30 sulfatide, loaded into MLD fibroblasts, when assayed by incubation at a dose level of 25 mU/ml in an assay as described in example 2 of WO 2005/073367. The ASA may in another embodiment in particular comprise: (a) the nucleic acid sequence of SEQ ID NO: 1 in WO 2005/073367 (b) a portion of the sequence in (a), which encodes an amino acid sequence, 35 which is enzymatically equivalent to recombinant human arylsulfatase A (c) a nucleic acid acid sequence analogue having at least 75% sequence identity to any one of the sequences in (a) or (b) and at the same time 11 encoding an amino acid sequence, which is enzymatically equivalent to recombinant human arylsulfatase A It may be preferred that the degree of sequence identity between the above mentioned nucleic acid sequence and SEQ ID NO: 1 of WO 2005/073367 is at 5 least 80%, such as at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, or at least 99%. It may be equally preferred that the degree of sequence identity between the amino acid sequence encoded by the above mentioned nucleic acid sequence and SEQ ID NO: 2 WO 2005/073367 is at least 80%, such as at least 85%, at least 90%, at least 95%, at least 97%, at least 10 98%, or at least 99%. For the purpose of the present invention it is preferred that the arylsulfatase A is a recombinant enzyme, particularly preferred is recombinant human arylsulfatase A (rhASA). It is preferred that rASA is produced in a mammalian cell or cell line and that said 15 mammalian cell or cell line produces a glycoform of rASA, which is efficiently endocytosed in vivo via the mannose-6-phosphate receptor pathway. Specifically, the preferred glycoform of rASA comprises an amount of exposed mannose-6 phosphate, which allows efficient endocytosis of rASA in vivo via the mannose-6 phosphate pathway. 20 In a particular embodiment at least one of the produced glycoforms of rASA is similar to a glycoform produced in CHO cells. The post translational modification of the cysteine residue in position 51 in the mature human arylsulfatase A is relevant for the activity of the enzyme. Accordingly, in a preferred embodiment of the present invention production of the 25 arylsulfatase A or its equivalent occurs at a rate and under conditions, which result in a product comprising an isoform of the enzyme in which the amino acid corresponding to Cys-69 in SEQ ID NO: 2 of WO 2005/073367 is converted to Formylglycine, corresponding to Fgly-51 in SEQ ID NO: 3 of WO 2005/073367. SEQ ID NO: 4 of WO 2005/073367 represents mature human arylsulfatase A after 30 cleavage of the 18 amino acid signal peptide but prior to modification of C-51. Thus in another embodiment of the present invention the ASA or its enzymatical equivalent may be selected from the group consisting of (a) the amino acid sequence of SEQ ID NO:3 of WO 2005/073367; (b) a portion of the sequence in (a), which is enzymatically equivalent to 35 recombinant human arylsulfatase A 12 (c) an amino acid sequence analogue having at least 75% sequence identity to any one of the sequences in (a) or (b) and at the same time being enzymatically equivalent to recombinant human arylsulfatase A. It may be preferred that the degree of sequence identity between the enzyme 5 produced according to the invention and SEQ ID NO: 3 of WO 2005/073367 or SEQ ID NO: 4 of WO 2005/073367 is at least 80%, such as at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, or at least 99%. For the biological activity and the effects of the enzyme in vivo requires to be optimal it is an advantage if an adequate amount of the enzyme has acquired a 10 glycosylation pattern as described above and has been modified post translationally at position 51. Thus at least 50%, 60%, 70%, 80%, 90%, 95% or 98% of the ASA of the present invention may be in the above described glycoform/isoform. The ASA of the present invention may in terms of its structure be different from 15 the rASA according to SEQ ID NO: 3 of 2005/073367. It may be an advantage that the sequence of amino acid residues surrounding the Cys-51 is identical or has a high degree of sequence identity to the corresponding sequence in SEQ ID NO: 3. Thus, it may be preferred that a linear sequence of 20 amino acids, such as 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 6, 5 or 4 amino acid residues 20 surrounding the Cys-51 in the arylsulfatase A is identical or at least 90% identical, such as 95%, 96%, 97%, 98%, or 99% identical to the corresponding sequence in SEQ ID NO: 3 of 2005/073367. As the active form of rASA within the lysosymes is an octamer the ASA of the present invention may in particular be a rASA which is an octamer or assembles into an octamer under physiological conditions. 25 The enzyme activity of ASA, which is to be understood as the catalytic activity of the rASA, may be measured in an enzyme assay based on the rASA mediated hydrolysis of either a detectable substrate or a substrate, which leads to a detectable end product. In a preferred aspect the assay is based on hydrolysis of the synthetic, chromogenic substrate, para-Nitrocatechol sulphate (pNCS) which 30 has an end product, para-Nitrocatechol (pNC) that absorbs light at 515 nm. Lysosomal alpha-mannosidase In yet another embodiment the polypeptide of interest may be a lysosomal alpha mannosidase (LAMAN). Lysomal alpha-mannosidase belongs to EC 3.2.1.24 and is an exoglycosidase which hydrolyses the terminal, non-reducing alpha-D-mannose 35 residues in alpha-D-mannosides from the non-reducing end during the ordered degradation of N-linked glycoproteins (Aronson and Kuranda FASEB J 3:2615- 13 2622. 1989). In the context of the present invention the term lysosomal alpha mannosidase may be used interchangeably with the short term LAMAN. The LAMAN of the present invention may in particular be of human origin. The human enzyme is synthesised as a single polypeptide of 1011 amino acids with a 5 putative signal peptide of 49 residues that is processed into three main glycopeptides of 15, 42, and 70 kD (Nilssen et al. Hum.Mol.Genet. 6, 717-726. 1997). The gene coding for LAMAN (MANB) is located at chromosome 19 (19cen-q12), (Kaneda et al. Chromosoma 95:8-12. 1987). MANB consists of 24 exons, 10 spanning 21.5 kb (GenBank accession numbers U60885-U60899; Riise et al. Genomics 42:200-207 . 1997). The LAMAN transcript is 3,500 nucleotides (nts) and contains an open reading frame encoding 1,011 amino acids (GenBank U60266.1). The cloning and sequencing of the human cDNA encoding LAMAN has been 15 published in three papers (Nilssen et al. Hum.Mol.Genet. 6, 717-726. 1997; Liao et al. J.Biol.Chem. 271, 28348-28358. 1996; Nebes et al. Biochem.Biophys.Res.Commun. 200, 239-245. 1994). Curiously, the three sequences are not identical. When compared to the sequence of Nilssen et al (accession # U60266.1) a TA to AT change at positions 1670 and 1671 resulting 20 in a valine to aspartic acid substitution was found by Liao et al. and Nebes et al. In a most preferred embodiment, the lysosomal alpha mannosidase comprises the amino acid sequence of SEQ ID NO.: 1 of WO 2005/094874. For practical and economical reasons it is preferred that the LAMAN of the present invention is produced recombinant. By recombinant production it may also be 25 possible to obtain a preparation of the enzyme wherein a large fraction contains mannose-6-phosphate. Recombinant production may be achieved after transfection of a cell using a nucleic acid sequence comprising the sequence of SEQ ID NO: 2 of WO 2005/094874. The alpha-mannosidase is preferably made in a mammalian cell system as this 30 will result in a glycosylation profile, which ensures efficient receptor mediated uptake in cells of for instance visceral organs of the body. In particular, it has been found that production of the enzyme in CHO, COS or BHK cells ensures adequate post-translational modification of the enzyme by addition of mannose-6 phosphate residues. In addition a correct sialylation profile is obtained. Correct 35 sialylation is known to be important in order to prevent uptake by the liver, because of exposed galactose residues.

14 In even more preferred embodiments the mammalian cell system is therefore selected from the group comprising CHO, COS cells or BHK cells (Stein et al. J Biol Chem.1989, 264, 1252-1259). It may further be preferred that the mammalian cell system is a human fibroblast cell line. 5 In a most preferred embodiment, the mammalian cell system is a CHO cell line. In another embodiment the lysosomal alpha-mannosidase may be a preparation of lysosomal alpha-mannosidase wherein a fraction of said preparation consists of lysosomal alpha mannosidase having one or more N-linked oligosaccharides carrying mannose 6-phosphate groups. 10 It is further preferred that a fraction of a preparation of said lysosomal alpha mannosidase is capable of binding to mannose 6-phosphate receptors. The ability of the enzyme to bind to mannose-6-phosphate receptors may be determined in an in vitro assay as described in example 1 of WO 2005/094874. Here, binding of the enzyme to a MPR affinity 300 Matrix provides a measure of 15 its ability to bind to mannose-6-phosphate receptors. In a preferred embodiment of the invention binding of the enzyme to mannose-6-phosphate receptors occurs in vitro. In more preferred embodiments of the invention this fraction corresponds to from 1 to 75% of the activity of a preparation of lysosomal alpha-mannosidase, such as 20 from 2 to 70%, such as from 5 to 60%, such as from 10 to 50% such as from 15 to 45%, such as from 20 to 40%, such as from 30 to 35%. Accordingly, it is preferred that the lysosomal alpha-mannosidase has a content of mannose 6-phosphate residues allowing mannose 6-phosphate dependent binding of from 2 to 100%, 5 to 95%, 10 to 90%, 20 to 80%, 30 to 70% or 40 to 60% of 25 the amount of enzyme to a Man-6-P-receptor matrix. At present, the degree of phosphorylation has been analysed in several batches of enzyme and, typically, from 30 to 45% of the enzyme is phosphorylated and binds the affinity matrix. It is further preferred that a fraction constituting from 2 - 1 0 0 %, 5 - 90%, 10 80%, 20 - 75%, 30 - 70%, 35 - 65% or 40 - 60% of the amount of said 30 lysosomal alpha-mannosidase binds to the Man-6-P-receptor with high affinity. Theoretically, two mannose 6-phosphate groups must be positioned close to each other in order for the enzyme to bind a Man-6-P-receptor with high affinity. Recent observations suggest that the distance between the phosphorylated mannose residues must be 40 A or less in order to obtain high affinity binding. In 35 the human lysosomal alpha-mannosidase according to SEQ ID NO: 1 of WO 15 2005/094874 the two mannose 6-phosphate residues may be situated at the asparagines residues in positions 367 and 766. Accordingly, it is preferred that the medicament according to the present invention comprises lysosomal alpha mannosidase, a fraction of which carries mannose 6-phosphate groups at both of 5 these asparagine residues. Preferably, the alpha-mannosidase is made by recombinant techniques. In a further embodiment, the alpha-mannosidase is of human origin (hLAMAN) and still more preferred a mature human alpha-mannosidase (mhLAMAN) or a fragment thereof. The fragment may be modified, however the active sites of the enzyme 10 should be preserved. It is to be expected that, in preparations of alpha-mannosidase according to the present invention, one fraction of the enzyme is represented by its precursor form, while other fractions represent the proteolytically processed forms of approximately 55 and 70 kDa. 15 Galactocerebrosidase In another embodiment the polypeptide of interest may be a galactocerebrosidase, which may be shortended to GALC. Galactocerebrosidase belongs to E.C. 3.1.6.46 and are enzymes capable of catalysing the reaction of D galactosyl-N-acylsphingosine + H20 = D-galactose + N-acylsphingosine, thus 20 GALC catalyzes the degradation of galactolipids in for example myelin. The GALC enzyme derived from humans is a glycosylated lysosomal enzyme comprising 643 amino acids and with a molecular weight of 72.8 kDa. The GALC of the present invention may in particular be of human origin. In a further embodiment the GALC may be expressed recombinant in one of the previously 25 mentioned host cells. The host cell for recombinant expression of GALC may in particular be a CHO cell. In the description and in the claims reference is made to the following amino acid and nucleic acid sequences: Sequence description Sequence identifier PBGD coding sequence 1 SEQ ID NO.: 1 PBGD coding sequence 2 SEQ ID NO.: 2 PBGD coding sequence 3 SEQ ID NO.: 3 PBGD coding sequence 4 SEQ ID NO.: 4 PBGD coding sequence 5 SEQ ID NO.: 5 PBGD coding sequence 6 SEQ ID NO.: 6 16 PBGD coding sequence 7 SEQ ID NO.: 7 PBGD coding sequence 8 SEQ ID NO.: 8 PBGD coding sequence 9 SEQ ID NO.: 9 PBGD coding sequence 10 SEQ ID NO.: 10 PBGD coding sequence, GenBank Acc. No. X04217 SEQ ID NO.: 11 PBGD coding sequence, GenBank Acc. No. X04808 SEQ ID NO.: 12 PBGD coding sequence, GenBank Acc. No. M95623 SEQ ID NO.: 13 PBGD aa sequence from coding sequence, GenBank SEQ ID NO.: 14 Acc. No. M95623, Constitutive form PBGD aa sequence from coding sequence, GenBank SEQ ID NO.: 15 Acc. No. M95623, Erythropoietic form ASA coding sequence Genbank Acc. No. J04593 SEQ ID NO.: 16 ASA coding sequence SEQ ID NO.: 1 of WO SEQ ID NO.: 17 2005/073367 ASA aa sequence SEQ ID NO.: 2 of WO 2005/073367 SEQ ID NO.: 18 ASA aa sequence SEQ ID NO.: 3 of WO 2005/073367 SEQ ID NO.: 19 ASA aa sequence SEQ ID NO.: 4 of WO 2005/073367 SEQ ID NO.: 20 LAMAN aa sequence SEQ ID NO.: 1 of WO SEQ ID NO.: 21 2005/094874 LAMAN coding sequence SEQ ID NO.: 1 of WO SEQ ID NO.: 22 2005/094874 Galactocerebrosidase coding sequence SEQ ID NO.: 23 Galactocerebrosidase aa sequence SEQ ID NO.: 24 With reference to these sequences the polypeptide of interest, according to preferred embodiments of the invention, comprises an amino acid selected from the group consisting of: i) an amino acid sequence as defined by any of SEQ ID NO.s: 14, 15, 18, 19, 5 20, 21 and 24; ii) a functionally equivalent part of an amino acid sequence as defined in i); and iii) a functionally equivalent analogue of an amino acid sequence as defined in i) or ii), the amino acid sequence of said analogue being at least 75% identical to an amino acid sequence as defined in i) or ii). 10 In particular embodiments the analogue in iii) is at least 80% identical to a sequence as defined in i) or ii), such as at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or such as at least 99.5% identical to a sequence as defined in i) or ii).

17 Furhter, the polypeptide of interest may be obtained by recombinant expression using a nucleic acid sequence comprising a sequence selected from the group consisting of: i) a nucleic acid sequence as defined by any of SEQ ID NO.s: 1-13, 16, 17, 22 5 and 23; ii) a nucleic acid sequence which is at least 75% identical to a nucleic acid sequence as defined in i). For recombinant production of the polypeptide it may further be preferred that the acid sequence in ii) is at least 80% identical to a sequence as defined in i), such 10 as at least 85%, at least 90%, at least 95%, at least 98%, at least 99%, or such as at least 99.5% identical to a sequence as defined in i). Composition comprising a polypeptide of interest The following description of a composition comprising a polypeptide of interest 15 relates both to a composition comprising a polypeptide which is concentrated according to a method of the present invention and it also relates to a composition of the present invention comprising at least 10 mg/ml polypeptide of interest. The present invention also relates to a composition comprising at least 10 mg/ml polypeptide of interest, wherein the polypeptide of interest may be any 20 polypeptide according to the present invention, such as in particular rhPBGD, aryl sulfatase, alpha-mannosidase or galactocerebrosidase. Said composition may in particular comprise at least 25 mg/ml polypeptide of interest, such as at least 50 mg/ml or at least 75 mg/ml or at least 100 mg/ml polypeptide of interest. Thus said composition may in particular comprise between 10-1000 mg/ml polypeptide 25 of interest, such as between 10-500 mg/ml or between 10-300 mg/ml or between 10-200 mg/ml or between 25-500 mg/ml or between 25-400 mg/ml or between 40-400 mg/ml or between 40-300 mg/ml or between 50-400 mg/ml or between 50-300 mg/ml or between 75-400 mg/ml or between 75-300 mg/ml or between 100-200 mg/ml or between 100-150 mg/ml polypeptide of interest. 30 The composition comprising a polypeptide of interest may in particular be an aqueous solution. Besides comprising a high concentration of polypeptide of interest said composition may in particular further comprise no aggregates of the polypeptide of interest or at least only very few aggregates. Hence the amount of polypeptide 35 of interest present as aggregates may in particular constitute less than 5 w/w% of the total amount of polypeptide of interest in the composition. In particular said 18 aggregates may constitute less than 4 w/w%, such as less than 3 w/w%, or less than 2 w/w%, or less than 1 w/w%, or less than 0.5 w/w%, or less than 0.1 w/w% of the total amount of polypeptide of interest. In the present context the term "aggregates" means any form of the polypeptide of interest which is not 5 monomeric. Thus the term encompasses any dimer or multimer of the polypeptide of interest. Furthermore, it is an advantage if said composition comprises only the polypeptide of interest or at least only minor traces of other proteins, i.e. proteins different from polypeptide of interest. Hence in a particular embodiment said 10 composition comprises less than 1 w/w%, such as less than 0.5 w/w%, or less than 0.1 w/w%, or less than 0.05 w/w%, or less than 0.01 w/w% other proteins than the polypeptide of interest. A range of factors affect the stability and activity of polypeptides and the composition comprising a polypeptide of interest may therefore in particular be 15 optimized to keep the polypeptide of interest as stable as possible. The pH generally affects the stability of a polypeptide of interest, thus the pH of a composition comprising a polypeptide of interest may in particular be in the range of 7.5-8.5, such as in particular between pH 7.7-8.2, more particularly between pH 7.8-8.0 or between pH 7.85-7.95, such as pH 7.8 or pH 7.9. This may in 20 particular be the case if the polypeptide of interest is PBGD. Thus the composition comprising a polypeptide of interest may in particular comprise a buffer capable of keeping the composition within the described pH range. Examples of such buffers include but are not limited to TRIS-HCL, Na Citrate and Na 2

HPO

4 . The concentration of such a buffer may depend on the 25 choice of the particular buffer and the presence of other components in the composition. If the buffer is Na 2

HPO

4 the concentration of Na 2

HPO

4 may be in the range of 0.5-15 mM, such as in the range of 1-10 mM, or in the range of 1.5-7.5 mM, such as in the range of 1.83-7.4 mM, or in the range of 1.5-3 mM, such as in the range of 1.83-3.7 mM, or in the range of 1.83-2.45 mM, or in the range of 30 3.5-7.5 mM, such as in the range of 3.6-7.4 mM, or in the range of 5.4-7.4 mM, such as 1.84 mM, or 2.45 mM, or 3.67 mM or 5.51 mM or 7.34 mM. If the buffer is TRIS-HCL the concentration of TRIS-HCL may in particular be in the range of 2-50 mM, such as 2-40 mM, or 2-30 mM, or 2-20 mM, or 2-10 mM, or 5-25 mM, or 5-20 mM, or 8-12 mM, or 9-11 mM, e.g. 10 mM. 35 Examples of other compounds which the composition comprising a polypeptide of interest may comprise include but are not limited to amino acids, sugars, alcohols 19 and detergents. Examples of such suitable compounds include but are not limited to glycine, mannitol, sucrose, L-serine, Tween 80 or a combination of one or more of said compounds. The concentration of these compounds depend on the particular compound, but for glycine the concentration may in particular be in the 5 range of 1-200 mM, such as in the range of 5-190 mM, or in the range of 10-180 mM, or in the range of 10-170 mM, or in the range of 20-160 mM, or in the range of 20-150 mM, or in the range of 25-125 mM, or in the range of 5-100 mM, or in the range of 5-90 mM, or in the range of 5-80 mM, or in the range of 5-70 mM, or in the range of 5-60 mM, or in the range of 10-100 mM, or in the range of 10-90 10 mM, or in the range of 10-80 mM, or in the range of 10-70 mM, or in the range of 10-60 mM, or in the range of 12-60 mM, or in the range of 12-55 mM, or in the range of 13.5-54 mM, or in the range of 10-30 mM, such as in the range of 13.5 27 mM, or in the range of 13.5-18 mM, or in the range of 25-55 mM, such as in the range of 27-54 mM, or in the range of 40-55, such as in the range of 40.5-54 15 mM, such as 12.5, 13, 13.5, 14, 14.5, 17, 17.5, 18, 18.5, 19, 25, 26, 27, 28, 29, 30, 39.5, 40, 40.5, 41, 41.5, or 53, 53.5, 53, 54.5 or 55 mM. The concentration of mannitol may in particular be in the range of 50-1000 mM, such as in the range of 50-900 mM, or in the range of 50-800 mM, or in the range of 50-700 mM, or in the range of 50-600 mM, or in the range of 100-900 mM, or 20 in the range of 100-800 mM, or in the range of 100-700 mM, or in the range of 100-600 mM, or in the range of 100-500 mM, or in the range of 120-525 mM, or in the range of 125-500 mM, or in the range of 100-300 mM, such as in the range of 120-275 mM, or in the range of 120-170 mM, or in the range of 200-600 mM, such as in the range of 225-550 mM, or in the range of 240-510 mM, or in the 25 range of 370-525 mM, such as 120, 125, 130, 160, 165, 166.7, 170, 175, 200, 221, 225, 250, 275,300, 365, 370, 375, 380, 385, 490, 495, 500, 505 or 510 mM. The concentration of sucrose may in particular be in the range ofl-200 mM, such as in the range of 5-190 mM, or in the range of 10-180 mM, or in the range of 10 30 170 mM, or in the range of 20-160 mM, or in the range of 20-150 mM, or in the range of 25-125 mM, or in the range of 5-100 mM, or in the range of 5-90 mM, or in the range of 5-80 mM, or in the range of 5-70 mM, or in the range of 5-60 mM, or in the range of 10-100 mM, or in the range of 10-90 mM, or in the range of 10 80 mM, or in the range of 10-70 mM, or in the range of 10-60 mM, or in the range 35 of 12-60 mM, or in the range of 12-55 mM, or in the range of 13.5-54 mM, or in the range of 10-30 mM, such as in the range of 13.5-27 mM, or in the range of 13.5-18 mM, or in the range of 25-55 mM, such as in the range of 27-54 mM, or in the range of 40-55, such as in the range of 40.5-54 mM, , such as 12.5, 13, 13.5, 14, 14.5, 17, 17.5, 18, 18.5, 19, 25, 26, 27, 28, 29, 30, 39.5, 40, 40.5, 41, 20 41.5, or 53, 53.5, 53, 54.5 or 55 mM. If sucrose is included in a composition which also comprises mannitol the concentration of mannitol may in particular be lowered corresponding to the concentration of sucrose; i.e. the concentration of mannitol and sucrose together may in particular be the same as the concentration 5 of mannitol if this was to be used alone. The concentration of Tween 80 may in particular be in the range of 0.001-1 w/v%, such as in the range of 0.005-1 w/v%, or in the range of 0.01-1 w/v%, or in the range of 0.001-0.5 w/v%, or in the range of 0.005-0.5 w/v%, or in the range of 0.01-0.5 w/v%, or in the range of 0.05-0.4 w/v%, or in the range of 10 0.05-0.3 w/v%, or in the range of 0.05-0.2 w/v%, or in the range of 0.075-0.4 w/v%, or in the range of 0.075-0.3 w/v%, or in the range of 0.075-0.2 w/v%, or in the range of 0.09-0.2 w/v%, such as 0.075, 0.08, 0.09, 0.1, 0.125, 0.15, 0.175 or 0.2 w/v%. The composition comprising a polyeptide of interest, wherein the polypeptide in 15 particular may be a PBGD, an aryl sulfatase, a lysosomal alpha-mannosidase or a galactocerebrosidase, may in particular comprise a combination of one or more of the above-mentioned compounds. A suitable example of such a composition may be one which besides the polypeptide of interest comprises Na 2

HPO

4 , glycine and mannitol. The pH of the composition and the concentration of the different 20 compounds may be as described above. Hence said composition may in one embodiment comprise 0.5-15 mM Na 2

HPO

4 , 1-200 mM glycine, 50-1000 mM mannitol and a pH in the range of 7.5-8.5. Any combination of the above mentioned concentrations of compounds and pH are encompassed by the present invention. A specific example of a suitable combination of other compounds and 25 pH in the composition comprising a polypeptide of interest is one which comprises 3.67 mM Na 2

HPO

4 , 27 mM glycine, 250 mM mannitol and has a pH in the range of 7.7 to 7.9. Other examples of suitable compositions include, but are not limited to any of the following: 30 - 1.84 mM Na 2

HPO

4 , 13.5 mM glycine, 125 mM mannitol and pH in the range of 7.7 to 7.9. * 2.45 mM Na 2

HPO

4 , 18 mM glycine, 167 mM mannitol and pH in the range of 7.7 to 7.9. * 5.51 mM Na 2

HPO

4 , 40.5 mM glycine, 375 mM mannitol and pH in the range 35 of 7.7 to 7.9. * 7.34 mM Na 2

HPO

4 , 54 mM glycine, 500 mM mannitol and pH in the range of 7.7 to 7.9. * 3.67 mM Na 2

HPO

4 , 27 mM glycine, 220 mM mannitol, 30 mM sucrose and pH in the range of 7.7 to 7.9.

21 * 3.67 mM Na 2

HPO

4 , 245 mM mannitol, 32 mM sucrose and pH in the range of 7.7 to 7.9. * 3.67 mM Na 2

HPO

4 , 27 mM L-serine, 250 mM mannitol and pH in the range of 7.7 to 7.9. 5 0 10 mM TRIS-HCI, 27 mM glycine, 250 mM mannitol and pH in the range of 7.7 to 7.9. * 3.67 mM NaCitrat, 27 mM glycine, 250 mM mannitol and pH in the range of 7.7 to 7.9. * 3.67 mM Na 2

HPO

4 , 27 mM glycine, 220 mM mannitol, 29 mM sucrose, 10 0.1%(w/v) Tween 80 and pH in the range of 7.7 to 7.9. * 3.67 mM Na 2

HPO

4 , 27 mM glycine, 220 mM mannitol, 29 mM sucrose, 0.1%(w/v) Tween 80 and pH in the range of 7.7 to 7.9. The composition comprising a polypeptide of interest may in particular be used for therapeutic applications in mammals. Thus the composition comprising a 15 polypeptide of interest may in particular be isotonic with regard to the tissue of mammals, e.g. it may in particular have an osmolality in the range of 200-400 mOsm/kg, such as in the range of 250-350 mOsm/kg or in the range of 275-325 mOsm/kg or in the range of 295-305 mOsm/kg, such as 295 mOsm/kg or 300 mOsm/kg or 305 mOsm/kg. 20 Method of concentrating a polypeptide of interest The method of the present invention comprises the steps of a) centrifugation and/or filtration of a composition comprising a polypeptide of interest and b) concentrating the composition from step a). The inventors of the present 25 invention have found that by centrifugation and/or filtrating a composition comprising a polypeptide of interest prior to concentrating said composition it is possible to obtain a composition comprising a highly concentrated polypeptide of interest without any or with at least only few aggregates of the polypeptide of interest. Furthermore, it is generally an advantage for therapeutic applications of 30 a polypeptide that the amount of polypeptide aggregates is reduced, e.g. as they may increase the risk of eliciting an immune response towards the polypeptide. For administration of a polypeptide subcutaneously it is an advantage that the polypeptide composition has a high activity in a small volume as only small volumes can be injected subcutaneously. 35 Proteins or polypeptides may in general form aggregates when they are concentrated. Thus it is an advantage that when the method of the present invention is used to concentrate a polypeptide of interest it does not cause a high 22 rate of polypeptide aggregate formation. As shown in the examples the amount of PBGD aggregates in the composition obtained by the concentration method of the present invention is similar to that of a non-concentrated PBGD composition. In a particular embodiment step a) of the method is performed prior to step b). 5 Step a) centrifugation and/or filtration The inventors of the present invention have found that prior to concentrating a composition comprising a polypeptide of interest it is an advantage to pre-treat the composition by centrifugation and/or filtration of the composition as by this 10 pre-treatment many or most of the polypeptide aggregates are removed. When the concentration of the composition in step b) is performed by a method which relies on the use of a filter or membrane, such as ultrafiltration, the presence of aggregates may block the filter or membrane so that small molecules and liquid are not able to cross the filter or membrane. This may decrease the 15 speed by which the composition is concentrated and/or completely block any further concentration. Hence for this type of concentration the pre-treatment according to step a) is an advantage as removal of the aggregates makes it possible to obtain compositions of a polypeptide of interest which are more concentrated than if said composition 20 were not been pre-treated. When the concentration of the composition in step b) is performed by a method which is based on the removal of water, such as freeze-drying or evaporation, the pre-treatment in step a) has the advantage that it reduces the amount of aggregates present in the concentrated composition. 25 Step a) may be performed by one of the following three alternatives: * Centrifugation, * Filtration, or * Centrifugation and filtration. If step a) comprises both centrifugation and filtration it is an advantage to 30 perform the centrifugation prior to the filtration as the inventors of the present invention have found that the centrifugation removes most of large aggregates and the filtration subsequently removes the remaining smaller aggregates.

23 Centrifugation To be able to remove the aggregates the composition comprising a polypeptide of interest may be centrifuged at a force in the range of 1500 - 3000 g, such as in the range of 1800-2500 g, or in the range of 2000-2300 g. 5 Typically the composition may be centrifuged for 10-60 minutes, such as for 15 50 minutes or for 20-40 minutes. As the temperature may affect the stability of the polypeptide of interest the centrifugation may be performed at a temperature in the range of 2-20 0 C, such as from 3-15 0 C or in the range of 3-10 0 C, or in the range of 3-8 0 C, such as at 4 0 C or 10 5oC or 6 0 C. The centrifugation results in that the polypeptide of interest aggregates sediment, i.e. they form a pellet, while the individual polypeptide of interest molecules stays in the solution. So it is the supernatant of the centrifuged composition which is subsequently used in the method of the present invention. 15 Filtration The composition comprising a polypeptide of interest may be filtered through a filter having a pore-size in the range of 0.20-5 pm, such as in the range of 0.2-2.5 Pm. Besides the pore-size of the filter also the material of which the filter is made of 20 may affect filtration of polypeptide of interest. Examples of suitable membrane filters include but are not limited to polyethersulfone (PES), cellulose acetate, regenerated cellulose and polyvinylidene flouride (PVDF). When molecules such as proteins are filtered it is usually the small molecules which are removed thus after filtration the polypeptide of interest may generally 25 be present in the retentate. Hence it is generally the retentate from the filtration which is used in the subsequent steps of the present invention. Step b) concentrating In principle any method of concentrating the polypeptide of interest composition 30 may be used in step b) of the present invention.

24 Examples of such suitable methods include but are not limited to ultrafiltration and concentration by removal of water. Ultrafiltration Ultrafiltration is a separation method in which hydraulic pressure is used to force 5 molecules and solvent across a membrane comprising pores of a particular size, also known as the cut-off size of value. Only molecules which have a molecular weight smaller than the cut-off value of the membrane are able to cross the membrane while those with a larger molecular weight do not cross the membrane and form the so called retentate. The molecules present in the retentate are 10 thereby concentrated as the solvent flows across the membrane. In a particular embodiment the concentration of the solution or composition comprising a polypeptide of interest may be performed by Tangential flow filtration (TFF). This method is in particular useful for large-scale concentration, i.e. for concentration of solutions with a volume from one litre to several hundreds 15 of litres. Thus this method is in particular useful for production of concentrated solutions of a polypeptide of interests on an industrial scale. The TFF technique is based on the use of a particular apparatus which causes the solution which is to be filtrated to flow across a semi-permeable membrane; only molecules which are smaller than the membrane pores will pass through the 20 membrane, forming the filtrate, leaving larger matter to be collected (retentate). With the TFF method two different pressures are applied; one to pump the solution into the system and to circulate it in the system (inlet pressure), and another pressure is applied over the membrane (membrane pressure) to force the small molecules and the solvent across the membrane. The inlet pressure may 25 typically be in the range of 1-3 bar, such as between 1.5-2 bar. The membrane pressure may typically be larger than 1 bar. The concentrated composition of a polypeptide of interest may be collected as the retentate when TFF is used to concentrate the composition. Membranes useful for TFF may typically be made of regenerated cellulose or 30 polyethersolufone (PES). The pore-size of the membrane may typically have a molecular weight cut-off which is smaller than 10.000 Mw, such as in the range of 10-10.000 Mw. In another embodiment the concentration of the composition comprising a polypeptide of interest may be performed by the use of a centrifugal device. The 35 principle of this method is that the solution is filtrated over a membrane by the 25 application of a centrifugal force over the membrane. Such membranes are often characterized by a molecular weight (Mw) cut-off, i.e. this is the maximum molecular size of compounds which are able to cross the membrane and compound with a molecular size larger than this will not cross the membrane. The 5 Mw cut-off of the membranes used in the present invention may in particular be smaller than 30.000 Mw, such as between 10-30.000 Mw. The membrane may in particular be made of polyethersulfone (PES) or regenerated cellulose. Examples of such suitable commercial filter devices may be Centricon Plus-80 or 10 Centricon Plus-15. The concentration may typically be performed by centrifugation at 2000-4500g, such as between 2500-4000g, or between 2750-3500g, or between 3000-3500g, such as at 3000g or 3100g or 3200g or 3300g or 3400g or 3500g. Typically the centrifugation may be run for several hours, e.g. for more than one 15 hour, such as for 1-10 hours. To minimize any negative effects on the stability of the polypeptide of interest the centrifugation may in particular be performed at a temperature in the range of 2 20 0 C, such as in the range of 3-15 0 C or in the range of 3-10 0 C or in the range of 3-6 0 C. 20 Concentrating by removal of water The principle of concentration by removal of water is usually that all, or most, of the water is removed to obtain a solid, and then subsequently diluting or dissolving this solid in a volume of water which is less than what it was previously diluted or dissolved in. However, it may in principle be performed by just 25 removing the necessary amount of water to obtain the desired concentration without subsequently re-diluting or re-dissolving the compound. Examples of suitable methods of concentrating by removal of water include freeze-drying and evaporation. Both for freeze-drying and evaporation the three most relevant parameters is the 30 temperature, pressure and the time. The method of freeze-drying may be comprise the following three or four steps; a freezing-phase, a primary drying phase and a secondary drying phase and optionally a step of annealing after the freezing phase. Freeze-drying may in 26 particular be performed as described with regard to freeze-drying included as a further step of the method of the present invention. Further steps 5 The polypeptide of interest may derive from a natural source, i.e. from cells naturally expressing the polypeptide of interest, or it may in particular be expressed recombinant. Independent of where the polypeptide of interest derives from it may have been purified before being subjected to a method of the present invention. 10 Such "purification" may in particular include but is not limited to removal of cell debris, removal of other proteins than polypeptide of interest and removal of other components which may be present in the source from which the polypeptide of interest is derived. Thus in a particular embodiment of the present invention the composition comprising a polypeptide of interest comprises less than 5 w/w%, 15 or less than 1 w/w% or less 0.5 w/w% or less than 0.1 w/w% or less than 0.05 w/w% or less than 0.01 w/w% other proteins than the polypeptide of interest. Thus other proteins which are expressed by e.g. a host cell may be removed from the composition comprising a polypeptide of interest before it is used in a method of the present invention. 20 Thus in a particular embodiment the method of the present invention may comprise one or more of following steps prior to step a): i) recombinant expression of a polypeptide of interest ii) purification of polypeptide of interest composition by one or more steps of chromatography 25 iii) exchange of the formulation buffer Recombinant expression of a polypeptide of interest may in particular be performed as described previously with regard to the polypeptide of interest. If the polypeptide of interest is PBGD examples of suitable types of chromatography include but are not limited to affinity chromatography, Ion 30 Exchange Chromatography (IEC) and chromatography on a hydroxyapatite column. In principle any combination of these chromatography methods may be used. The inventors of the present invention have previously found for PBGD that it is an advantage to perform at least the step of affinity chromatography and if this is combined with any of the other methods of chromatography it is an 27 advantage to perform the step of affinity chromatography prior to the other chromatography steps (see e.g. WO 03/002731). For the embodiment where the polypeptide of interest is PBGD examples of commercially available affinity chromatography columns include affinity coupling, 5 group specific affinity, and metal chelate affinity columns. The product catalogue 2001 of the company Amersham Pharmacia Biotech gives examples of affinity coupling columns such as columns comprising immobilising ligands containing -NH 2 and columns comprising ligands containing primary amino groups. 10 Metal chelate affinity columns are specially preferred for purifying proteins via metal ion complex formation with exposed histidine groups. Example 3 of W001/07065 describes construction of a recombinant human Porphobilinogen deaminase with a "His-Tag" (rhPBGD-His). In order to purify rhPBGD-His it is preferred to use a metal chelate affinity column, such as a column having a cobalt 15 metal affinity resin. Examples of other suitable methods of affinity chromatography include but are not limited to columns having porcine heparin as ligand or columns having 1-Amino-4 [[4-[[4-chloro-6-[[3 (or 4)-sulfophenyl]amino]-1,3,5-triazin-2-yl]amino]-3 sulfophenyl]amino]-9,10-dihydro-9,10-dioxo-2-anthracenesulfonic acid, also 20 known as Cibracon Blue 3G, as ligand and using Triazine coupling as the ligand coupling method. A commercially available example of the latter is Blue Sepharose 6 Fast Flow (FF) from Amersham Pharmacia Biotech. Accordingly, a preferred embodiment of the invention relates to the process, as described herein, wherein the affinity chromatography column of step (i) is a column using a 25 triazine coupling as ligand coupling method, and more preferably wherein the ligand is Cibacron Blue 3G. The term "Ion Exchange Chromatography (IEC)" should herein be understood according to the art as a column separating molecules such as proteins on the basis of their net charge at a certain pH by electrostatic binding to a charged 30 group on the column. Ion exchange denotes the absorption of ions of one type onto a column in exchange for others which are lost into solution. Examples of suitable IEC columns are columns such as a Q Sepharose column, a Q SP Sepharose column, or a CM Sepharose column, it may in particular be a DEAE Sepharose column.

28 An example of a suitable hydroxyapatite column is a ceramic hydroxyapatite column. Hydroxyapatite (Ca 5

(PO

4 )30H) 2 is a form of calcium phosphate that can be used for the separation and purification of proteins, enzymes, nucleic acids, viruses, and other macromolecules. Ceramic hydroxyapatite is a spherical, 5 macroporous form of hydroxyapatite. CHT Type I (Bio-Rad) is an example of a suitable commercially available ceramic hydroxyapatite chromatography column. In one embodiment the method of the present invention may comprise the following steps prior to step a): i) recombinant expression of PBGD 10 ii) subjecting the PBGD composition from step i) to affinity chromatography iii) subjecting the PBGD composition of step ii) to ion exchange chromatography In a further embodiment the method of the present invention may comprise the 15 following steps prior to step a): i) recombinant expression of PBGD ii) subjecting the PBGD composition from step i) to affinity chromatography iii) subjecting the PBGD composition from step ii) to ion exchange 20 chromatography iv) subjecting the PBGD composition from step iii) to a hydroxyapatite column Both of these methods may optionally include a further step of dilution of diafiltration of the PBGD composition obtained from step ii). Thus said step should 25 be after step ii) and before iii), i.e. a step iia). Step iia) has the purpose of reducing the concentration of salts to suitable conductivity, e.g. < 10 mS/cm. This may in particular be relevant if DEAE Sepharose is used as resin in the ion exchange chromatography step, i.e. step iii), as this may facilitate binding of the captured PBGD to the DEAE Sepharose resin. Dilution may be obtained by addition 30 of purified water directly or by ultrafiltration against purified water. The recombinant expression of PBGD, step i) may be performed by any of the methods described above. Examples of suitable affinity chromatography columns in step ii) may be any of the above mentioned. 35 Examples of suitable methods of performing ion exchange chromatography in step iii) may be any of the above mentioned.

29 Examples of suitable hydroxyapatite chromatography columns in step iv) may be any of the above mentioned. In a particular embodiment the affinity chromatography column may be a column using a triazine coupling as ligand coupling method, and in particular such a 5 method wherein the ligand is Cibracon Blue 3G. This may in particular be a Blue Sepharose 6 Fast Flow column, and the ion exchange chromatography column may be DEAE Sepharose column, and in the embodiment wherein the method also comprises a step iv) this column may in particular be a ceramic hydroxyapatite column. 10 The method of the present invention may also comprise further steps after step b) of the method. Such steps include but are not limited to one or more of the following: * freeze-drying the composition comprising a concentrated polypeptide of interest, 15 0 changing the buffer of the composition comprising a concentrated polypeptide of interest, * sterile filtration of the composition comprising a concentrated polypeptide of interest * evaporation 20 Different freeze-driers, volume of solutions to be freeze-dried and other parameters may be used in the method of the present invention. An example of a suitable freeze-dryer includes but is not limited to a Lyostar (FTM-systems) freeze-drier as used the examples of the present invention, where the solutions comprising a concentrated polypeptide of interest, i.e. in this case PBGD, were 25 filled in 2 and 6 ml injection glass vials (type 1) and stoppered with rubber stoppers (chlorobutyl). The freeze-drying may be performed by the following three steps; i) freezing, ii) primary drying, and 30 iii) secondary drying . Step i) freezing may in particular be performed by first loading a sample in ambient temperature and cooling it to 0 0 C and keeping it at 0 0 C for 30 minutes, before lowering the temperature by 1 0 C per minute to -40 0 C and keeping it at 40 0 C for 30 minutes. 35 Step ii) primary drying may in particular be performed by drawing the vacuum pressure 126 mTorr, raising the temperature by 1 0 C per minute to 0 0 C and keeping the sample at 0 0 C for 360 minutes 30 Step iii) secondary drying may in particular be performed by drawing the full vacuum simultaneously with raising the temperature by 0.5 0 C per minute to +30 0 C and keeping the sample at +30 0 C for 360 minutes. After the secondary drying the sample may further be closed under vacuum or 5 closed after filling with nitrogen. An example of a suitable freeze-drying method includes the one described in the examples of the present invention. The freeze-drying may in further embodiment comprise an annealing step prior to the primary drying phase. The inventors of the present invention have found that 10 inclusion of an annealing step in the freeze-drying method improves the visual appearance, as visualised by fewer cracks, and/or results in a shorter reconstitution time of the freeze-dried product compared to when the same method of freeze-drying is used but without the annealing step. It is an advantage that the time for reconstitution of a freeze-dried product is reduced, especially if it 15 is to be used as a pharmaceutical which is administered as a solution. An improved visual appearance is usually also regarded as an advantage for most products. Thus the freeze-drying may comprise the following steps: i) freezing 20 ii) annealing iii) primary drying iv) secondary drying. The freezing, primary drying and secondary drying steps may in particular be performed as described above. The annealing step, i.e. step ii) may in particular 25 be performed by after 30 minutes of freezing, raising the temperature at e.g. a rate of 2 0 C per minute to -10 0 C or -20 0 C and keeping this temperature for 120 or 420 minutes and then lowering the temperature e.g. a rate of 2 0 C per minute to 40 0 C at which temperature the sample may be kept at 60-90 minutes before start of the step of primary drying. 30 Changing the buffer of the composition comprising a concentrated polypeptide of interest may in particular be performed by a) diluting, e.g. 5-15 times, the composition comprising a concentrated polypeptide of interest in a buffer or formulation, b) concentrating the diluted composition again and performing the steps a) and b) a sufficient number of times so that amount of the excipients in 35 the buffer or formulation present in the composition before these steps constitute 31 less than e.g 5 v/v% or less than 1 v/v% of excipients in the the buffer or formulation present in said composition after said steps were performed. In particular the composition comprising a polypeptide of interest obtained from step b) of the present invention may in particular further comprise a step of sterile 5 filtration of said composition and/or a step of freeze-drying the composition. Sterile filtration is generally performed by filtration of the composition through a filter with a pore-size of 0.22 pm or 0.20 pm. Freeze-drying may in particular be performed as described above. The present invention also relates to a freeze-dried composition obtained by a 10 method of the present invention. Subcutaneous injection The present invention also relates to the use of a composition comprising in the range of 50-300 mg/ml polypeptide of interest for the manufacture of a 15 medicament for subcutaneous injection into a mammal. The polypeptide of interest may be any polypeptide of interest according to the present invention, including but not limited to PBGD, aryl sulfatase A, lysosomal alpha-mannosidase and galactocerebrosidase. The term subcutaneous is often shortened to s.c. and the two terms may be used 20 interchangeably in the context of the present invention. When injection is performed subcutaneously it is usually not possible to inject more than 1.5 mL due to physiologically restraints. As the patient usually needs a certain amount of the particular polypeptide of interest there is a correlation between the volume of the composition comprising a 25 polypeptide of interest which needs to be administered to the patient and of the concentration of polypeptide of interest in said composition. It is therefore an advantage of the present invention that the composition comprising a polypeptide of interest comprises a high concentration of the polypeptide of interest and that this high concentration of the polypeptide of 30 interest can be obtained without the formation of large amounts of polypeptide aggregates. The use of such concentrated polypeptide of interest compositions makes it possible to inject a smaller volume of said composition and at the same time ensure that the patient receives an adequate amount of the polypeptide of 32 interest; thus making it easier to administer the polypeptide of interest subcutaneously. The above-mentioned composition comprising a polypeptide of interest may in particular comprise between 75-250 mg/ml, such as between 75-200 mg/ml or 5 between 75-150 mg/ml or between 100-150 mg/ml or between 100-125 mg/ml or between 125-150 mg/ml of polypeptide of interest. As described above the volume of composition comprising a polypeptide of interest which it is necessary to inject into the patient to ensure that the patient recieves an adquate amount of the polypeptide of interest correlates with the 10 concentration of the polyeptide of interest in said composition. Thus the volume of such a composition will generally be adjusted according to the concentration of the polypeptide of interest in the composition. However, the volume may generally be in the range of 0.1-1.5 ml, such as in the range of 0.1 1.5 ml or in the range of 0.5-1.5 ml or in the range of 0.5-1.5 ml or in the range 15 of 0.75-1.5 ml or in the range of 0.75-1.5 ml or in the range of 1-1.5 ml or in the range of 1-1.5 ml. The amount of polypeptide of interest which it is relevant to administer to a patient generally depends on the weight of the individual and the particular polypeptide of interest. 20 In one embodiment the present invention relates to a method of treating a mammal for Acute Intermittent Porphyria comprising subcutaneous injection of a composition of 50-300 mg/ml PBGD. Administration of PBGD may in particular be useful for the treatment of Acute Intermittent Porphyria. However, it is contemplated that administration of PBGD 25 also may be useful for the treatment of other porphyrias, such as Hereditary coproporphyria or Variegata porphyria. Porphyria is a term used to collectively describe a number of diseases caused by different deficiencies in the heme biosynthetic pathway. Hence it is contemplated that administration of PBGD, e.g. in combination with other therapeutics, to a patient suffering from any type of 30 porphyria may help to increase the overall turnover of the different intermediates in the pathway. For example Meissner PN et al., 1986, European Journal of Clinical Investigation, vol. 16, 257-261; Hift RJ et al., 1997, S. Afr. Med. J., vol. 87, 718 27 and Meissner P et al., 1993, J. Clin. Invest., vol. 91, 1436-44 describe accumulation of ALA and PBG in Hereditary coproporhyria and Variegata 35 porphyria. In theses diseases the accumulation of ALA and PBG results from enzymatic defects that are located four and five steps downstream form the 33 conversion of ALA to PBG, respectively. In the two most recent papers it is described how the porphyrinogen which accumulates in patients with Variegata porphyria is capable of inhibiting PBG-deaminase. In a further embodiment the present invention relates to a method of treating a 5 mammal for metachromatic leukodystrophy comprising subcutaneous injection of a composition of 50-300 mg/ml aryl sulfatase A. Metachromatic leukodystrophy (MLD) is caused by an autosomal recessive genetic defect in the lysosomal enzyme Arylsulfatase A (ASA), resulting in a progressive breakdown of membranes of the myelin sheath (demyelination) and accumulation 10 of galactosyl sulphatide (cerebroside sulphate) in the white matter of both the central nervous system (CNS) and the peripheral nervous system. In histologic preparations, galactosyl sulphatide forms spherical granular masses that stain metachromatically. Galactosyl sulphatide also accumulates within the kidney, gallbladder, and certain other visceral organs and is excreted in excessive 15 amounts in the urine. Galactosyl sulfatide is normally metabolised by the hydrolysis of 3-0-sulphate linkage to form galactocerebroside through the combined action of the lysosomal enzyme arylsulfatase A (EC 3.1.6.8) (Austin et al. Biochem J. 1964, 93, 15C-17C) and a sphingolipid activator protein called saposin B. A profound deficiency of 20 arylsulfatase A occurs in all tissues from patients with the late infantile, juvenile, and adult forms of MLD (see below). In the following, the arylsulfatase A protein will be termed "ASA". A profound deficiency of ASA occurs in all tissues from patients with MLD. In yet another embodiment the present invention relates to a method of treating a 25 mammal for the lysosomal storage disorder alpha-mannosidosis comprising subcutaneous injection of a composition of 50-300 mg/ml lysosomal alpha mannosidase. Alpha-mannosidosis is a recessive, autosomal disease that occurs world wide with a frequency of between 1/1.000.000 and 1/500.000. Mannosidosis is found in all 30 ethnic groups in Europe, America, Africa and also Asia. It is detected in all countries with a good diagnostic service for lysosomal storage disorders, at a similar frequency. They are born apparently healthy; however the symptoms of the diseases are progressive. Alpha-mannosidosis displays clinical heterogeneity, ranging from very serious to very mild forms. Typical clinical symptoms are: 35 mental retardation, skeletal changes, impaired immune system resulting in recurrent infections, hearing impairment and often the disease is associated with a typical facial characteristics such as a coarse face, a prominent forehead, a 34 flattened nasal bridge, a small nose, and a broad mouth. In the most severe cases (mannosidosis type I) the children suffer from hepatosplenomegaly, and they die during the first years of life. Possibly this early death is caused by severe infections due to the immunodeficiency caused by the disease. In milder cases 5 (mannosidosis type 2) the patients usually reach adult age. The skeletal weaknesses of the patients result in the needs of wheeling chairs at age 20 to 40. The disease causes a diffuse dysfunction of the brain often resulting in weak mental performances that excludes anything but the most basic skills of simple reading and writing. These problems associated with hearing inabilities and other 10 clinical manifestations preclude the patient from an independent life, the consequence being that life long caretaking is needed. In yet another embodiment the present invention relates to a method of treating a mammal for Krabbe disease comprising subcutaneous injection of a composition of 50-300 mg/ml galactosylcerebrosidase. 15 In humans a deficiency in the GALC enzyme results in an autosomal inherited genetic Lysosomal Storage disease known as Krabbe disease or Globoid Cell Leukodystrophy. The enzyme is generally expressed in the testis, kidneys, placenta, liver and brain of human beings and a deficiency in the GALC enzyme generally results in a disorder in the myelin metabolism and in the central and 20 peripheral nervous systems (the CNS and PNS, respectively). Krabbe disease has been observed in humans of any age, nationality and sex. It should be noted that embodiments and features described in the context of one of the aspects of the present invention also apply to the other aspects of the invention. In particular, all of the embodiments described for the composition 25 comprising a polypeptide of interest, such as the presence of further compounds, buffers and pH also apply to the composition comprising a polypeptide of interest used in the present applications. When an object according to the present invention or one of its features or 30 characteristics is referred to in singular this also refers to the object or its features or characteristics in plural. As an example, when referring to "a polypeptide" it is to be understood as referring to one or more polypeptides. Throughout the present specification the word "comprise", or variations such as 35 "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the 35 exclusion of any other element, integer or step, or group of elements, integers or steps. All patent and non-patent references cited in the present application, are hereby 5 incorporated by reference in their entirety. The invention will now be described in further details in the following non-limiting Experimental sections. 10 EXPERIMENTAL Materials rhPBGD The rhPBGD used in the following experiments were obtained according to process 2 in example 1 of WO 03/002731, where process 2 is the process which includes 15 step IV, i.e. the ceramic hydroxyapatite chromatography step. Formulation buffer The recombinant and purified rhPBGD was present in the following aqueous formulation buffer: 3.67 mM Na 2

HPO

4 20 27 mM Glycine 250 mM Mannitol and a pH of 7.9 The formulation buffer was then sterile-filtered trough a 0.22 pm filter. Methods 25 Freeze-drying The freeze-drying of the purified rhPBGD solutions were performed in a Lyostar (FTM-systems) freeze-drier according to the following schedule: Freezing phase OC 30 min 760 Torr 0 0 C to -40 0 C 1 0 C/min 760 Torr -40 0 C 30 min 760 Torr Primary drying -40 0 C to OC 1 0 C/min 169 mTorr 0 0 C 240 min 169 mTorr 36 Secondary drying 0 0 C to 30 0 C 10 0 C/60 min, 180 20 mTorr min 30 0 C 720 min 20 mTorr Visual observation (Clarity and colour) The liquid was visually studied with respect to colour, clarity and precipitates according to the scheme below. 5 Colour: 1: No colour; 2: Slightly yellow; 3: Yellow Clarity: 1: Clear; 2: Slightly turbid; 3: Turbid Other remarks: Other observations from the operator were in some instances included here (e.g. precipitates, undissolved material etc) rH-measurement 10 The pH-meter (Metrohm 691 pH Meter) and electrode (combined LL pH electrode) were calibrated with 3 standard reference solutions (Merck) in the range 4.00 to 9.00. The liquid was finally analysed. Protein concentration Protein concentration in extract, in-process samples, bulk drug substance and 15 final product was determined by a method that utilizes principles of the reduction of Cu2+ to Cu+ by protein in an alkaline medium (the Biuret reaction). The Cu+ ions were then reacted with a reagent containing bicinchoninic acid resulting in a highly sensitive and selective colorimetric detection. Purity 20 Recombinant human Porphobilinogen Deaminase (rhPBGD) and rhPBGD variants were separated according to their ability to adsorb and desorb to silica based stationary media depending on the percentage of organic modifier (acetonitrile) in the mobile phase. rhPBGD activity 25 Porphobilinogen deaminase (PBGD) catalyzes the addition of 4 molecules of porphobilinogen (PBG) to form a linear tetramer, preuroporphyrinogen, which is released from the enzyme and in vivo circularized to uroporphyrinogen III by the action of Uroporphyrinogen III synthase. Preuroporphyrinogen can be chemically oxidized with benzoquinone to form uroporphyrin, which absorbs light at 405 nm.

37 The analyses were performed on one single vial on each test occasion. For the determination of rhPBGD activity and protein concentration the tests were performed in duplicate and triplicate respectively, for each vial. Osmolality 5 One vial of freeze-dried rhPBGD was resuspended in 1.00 ml MilliQ-water. The vial of frozen aqueous solution of rhPBGD was thawed. The osmometer (Vapro osmometer) was calibrated with 3 standard solutions in the range 100-1000 mOsm/kg (100, 290, 1000 mOsm/kg). The liquid was then analyzed. 10 Example 1 Concentrating with centrifugal filter devices Frozen PBGD-bulk solution (7 mg/mL rhPBGD, 3.67 mM Na 2

HPO

4 , 27 mM glycine, 250 mM Mannitol, pH 7.9) was thawed in a water-bath at 20 OC, centrifuged at 3200g for 10 min and thereafter sterile-filtrated by 0.20 pm-PES filters (Nalgene 15 Polyethersulfone filters). The PBGD-bulk solution was concentrated to 100 mg/ml by running the Centrifugal Filter Devices Centricon Plus-80 (Mw cut-off 30000) and Centricon Plus-15 (Mw cut-off 30000) at 3200 g for several hours. The concentrated solution, i.e. the retentate, was sterile-filtrated by 0.22 pm-filters (Millex GV) and finally a part of this solution was diluted with sterile formulation 20 buffer to get 50 mg/ml. The 5 mg/ml-solution was prepared by directly diluting the recombinant and purified hPBGD with sterile formulation buffer. The 5 mg/mL, 50 mg/mL and 100 mg/mL rhPBGD were then freeze-dried as described above. Several vials of each the above-mentioned freeze-dried rhPBGD solutions with 5, 50 and 100 mg/mL rhPBGD and of the aqueous 5 mg/mL 25 rhPBGD solution were stored at 40 0 C 2 0 C, 75% ± 5% relative humidity (RH). The vials were stored protected from light in a well sealed secondary package (paper box). At the indicated time points (i.e. time of storage) a vial of each freeze-dried samples were resuspended in 1.00 mL Millipore water. 30 Each of the resuspended vials and the aqueous vial of rhPBGd were then visually observed with regard to colour, clarity and precipitates, and the pH, protein concentration, purity and rhPBGD activity were measured as described above. The results are given in the following tables 1-4: 38 Table 1: Freeze-dried product, 5 mg/mL Time-point Activity Concentration Specific Purity (%) Visual (month) (U/ml) (mg/ML) activity observation (U/mg) 0 93.2 4.3 21.5 99.6 Colour: 1, clarity: 1 0.5 81.0 5.2 15.6 ND Colour: 1, clarity: 1 1 76.6 5.9 13.1 99.9 Colour: 1, clarity: 1 1.5 87.0 5.5 15.9 99.7 Colour: 1, clarity: 1 2 53.3 4.7 11.4 99.6 Colour: 1, clarity: 1 3 50.8 4.8 10.7 99.6 Colour: 1, clarity: 1 6 34.3 5.3 6.5 99.6 Colour: 1, clarity: 1 5 Table 2; freeze-dried product; 50 mg/ml Time-point Activity Concentration Specific Purity (%) Visual (month) (U/ml) (mg/ML) activity observation (U/mg) 0 888 41.4 21.5 99.1 Colour: 2, clarity: 1 0.5 842 50.6 16.6 ND Colour: 2, clarity: 1 1 746 50,6 14.8 100 Colour: 2, clarity: 1 2 640 52.9 12.1 100 Colour: 2, clarity: 1 3 634 49.0 12.9 100 Colour: 2, clarity: 1 6 422 43.0 9.8 100 Colour: 2, clarity: 1 Table 3: Freeze-dried product; 100 mg/ml 39 Time-point Activity Concentration Specific Purity (%) Visual (month) (U/ml) (mg/ML) activity observation (U/mg) 0 1944 83.7 23.2 99.1 Colour: 3, clarity: 1 1 1470 98.7 14.9 100 Colour: 3, clarity: 1 2 1282 94.8 13.5 100 Colour: 3, clarity: 1 3 1253 82.6 15.2 100 Colour: 3, clarity: 1 6 739 75.5 9.8 100 Colour: 3, clarity: 1 Table 4: Aqueous product; 5 mg/ml Time-point Activity Concentration Specific Purity (%) Visual (month) (U/ml) (mg/ML) activity observation (U/mg) 0 95.6 4.0 23.7 99.1 Colour: 1, clarity: 1 0.5 48.1 5.4 8.9 ND Colour: 1, clarity: 1 1 28.6 5.9 4.8 96.1 Colour: 1, clarity: 1 1.5 12.3 5.6 2.2 91.4 Colour: 1, clarity: 1 2 4.5 4.4 1.0 90.7 Colour: 1, clarity: 1 3 7.1 3.1 2.3 87.3 Colour: 2, clarity: 2 6 4.4 2.1 2.1 58.1 Colour: 2, clarity: 2 5 Example 2 Concentrating a rhPBGD composition by centrifugal filter devices 40 Frozen PBGD-bulk solution (7 mg/mL rhPBGD, 3.67 mM Na 2

HPO

4 , 27 mM glycine, 250 mM Mannitol, pH 7.9) was thawed in a water-bath at 20 4C, centrifuged at 3200g for 10 min and thereafter sterile-filtrated by 0.20 pm-PES filters (Nalgene Polyethersulfone filters). The PBGD-bulk solution was concentrated to 100 mg/ml 5 by running the Centrifugal Filter Devices Centricon Plus-80 (Mw cut-off 30000) and Centricon Plus-15 (Mw cut-off 30000) at 3200 g for several hours. The concentrated solution, i.e. the retentate, was sterile-filtered by 0.22 pm-filters (Millex GV) and diluted with sterile filtered formulation buffer (see above) to get solutions of lower concentrations. A fraction 10 in volume of each concentration was freeze-dried as described above. The different concentrations of freeze-dried rhPBGD and aqueous solution of rhPBGD were stored at 54C ± 3 0 C or at -20 0 C ± 5 0 C (ambient relative humidity (RH)). All vials were stored protected from light in a well-sealed secondary package (paper box). 15 At the indicated time points (i.e. time of storage) a vial of each freeze-dried samples were resuspended in 1.00 mL Millipore water and then tested together with the aqueous solution of rhPBGD by visually observing the colour, clarity and precipitates, and by measuring pH, protein concentration, purity, osmolality and rhPBGD activity. 20 The results are given in the following tables 5-19: Table 5: Aqueous product; 11 mg/ml; Storage temp.:+5 0 C Time- Protein Activity Specific Purity PH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 10.9 255.0 23.4 100.0 7.80 290 Colour: 2 Clarity: 1 Clear None/few 1 9.5 216.8 22.8 100.0 7.81 305 Colour: 2 Clarity: 1 Clear None/few 41 2 10.9 230.2 21.1 98.0 7.80 300 Colour: 2 Clarity: 1 Clear None/few 3 11.2 226.6 20.2 100.0 7.76 290 Colour: 2 Clarity: 1 Clear Few 6 14.7 271.1 18.4 100.0 7.77 300 Colour:2 Clarity: 1 Clear Several Table 6: Aqueous product: 11 mg/ml; Storage temp: -20 0 C Time- Protein Activity Specific Purity PH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 10.4 236.1 22.6 100 7.80 290 Colour: 2 Clarity: 1 Clear None 1 11.7 270.3 23.1 100 7.81 302 Colour: 2 Clarity: 1 Clear None 2 ND ND ND ND ND ND ND 3 12.4 247.7 20.0 100 7.77 288 Colour: 2 Clarity: 1 Clear None 6 13.4 291.5 21.8 100 7.77 301 Colour: 2 Clarity: 1 Clear None 42 Table 7: Freeze-dried product, 11 mg/ml; Storage temp.: +5 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3; Clarity 1-3; Solution; Aggregates 0 10.9 230.0 21.2 100.0 7.80 290 Colour: 2 Clarity: 1 Clear None 1 ND ND ND ND ND ND ND 2 ND ND ND ND ND ND ND 3 13.3 269.3 20.2 100.0 7.74 282 Colour: 2 Clarity: 1 Clear None 6 14.7 237.9 16.2 100.0 7.76 290 Colour: 2 Clarity: 1 Clear None 5 Table 8: Aqueous product, 17 mg/ml; Storage temp.: +5 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 18.0 471.0 26.1 100.0 7.80 298 Colour: 2 Clarity: 1 Clear None/few 1 17.5 360.4 20.6 100.0 7.81 311 Colour: 2 Clarity: 1 Clear 43 None/few 2 18.3 397.0 21.7 100.0 7.83 302 Colour: 2 Clarity: 1 Clear None/few 3 16.6 376.5 22.7 100.0 7.77 294 Colour: 2 Clarity: 1 Clear Few 6 16.0 257.3 16.1 100.0 7.76 305 Colour: 2 Clarity: 1 Clear Several Table 9: Aqueous product, 17 mg/ml; Storage temp.: -20 0 C Time- Protein Activity Specific Purity PH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 17.9 411.6 23.0 100.0 7.80 298 Colour: 2 Clarity: 1 Clear None 1 17.4 439.5 25.3 100.0 7.80 310 Colour: 2 Clarity: 1 Clear None 2 ND ND ND ND ND ND ND 3 16.4 389.4 23.7 100.0 7.77 292 Colour: 2 Clarity: 1 Clear None 6 18.0 373.8 20.8 100.0 7.76 305 Colour: 2 Clarity: 1 Clear 44 None Table 10: Freeze-dried product, 17 mg/ml; Storage temp.: 5 0 C Time- Protein Activity Specific Purity PH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 16.9 380.1 22.5 100.0 7.80 298 Colour: 2 Clarity: 1 Clear None 1 ND ND ND ND ND ND ND 2 ND ND ND ND ND ND ND 3 15.6 391.9 25.1 100.0 7.76 285 Colour: 2 Clarity: 1 Clear None 6 16.6 341.3 20.6 100.0 7.75 297 Colour: 2 Clarity: 1 Clear None 5 Table 11: Aqueous product; 36 mg/ml; Storage temp.:+5 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 36.0 844.4 23.4 100.0 7.81 305 Colour: 2 Clarity: 1 Clear None/few 1 35.5 778.1 21.9 100.0 7.82 314 Colour: 2 Clarity: 1 45 Clear None/few 2 35.4 798.5 22.6 100.0 7.81 310 Colour: 2 Clarity: 1 Clear None/few 3 28.9 687.9 23.8 100.0 7.77 303 Colour: 2 Clarity: 1 Clear Few 6 37.2 537.3 14.4 100.0 7.77 312 Colour: 2 Clarity: 1 Clear Several Table 12: Aqueous product, 36 mg/ml; Storage temp.: -20 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 34.0 853.4 25.1 100.0 7.81 305 Colour: 2 Clarity: 1 Clear None 1 38.0 853.6 22.5 100.0 7.83 321 Colour: 2 Clarity: 1 Clear None 2 ND ND ND ND ND ND ND 3 31.6 776.3 24.6 100.0 7.76 299 Colour: 2 Clarity: 1 Clear None 6 30.6 543.8 17.8 100.0 7.75 311 Colour: 2 Clarity: 1 Clear 46 None Table 13: Freeze-dried product, 36 mg/ml; Storage temp.: 5 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 29.5 657.0 22.3 100.0 7.81 305 Colour: 2 Clarity: 1 Clear None 1 ND ND ND ND ND ND ND 2 ND ND ND ND ND ND ND 3 28.7 747.6 26.0 100.0 7.75 290 Colour: 2 Clarity: 1 Clear None 6 29.8 579.3 19.4 100.0 7.76 300 Colour: 2 Clarity: 1 Clear None 5 Table 14: Aqueous product, 50 mg/ml; Storage temp.: 5 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 46.2 780.9 16.9 96.3 7.59 317 Colour: 3 Clarity: 1 Slightly opalescent None 1 47.9 915 19.1 90 7.58 305 Colour: 3 47 Clarity: 1 Slightly opalescent None 2 47.2 898.3 19.0 100 7.60 318 Colour: 3 Clarity: 1 Slightly opalescent None 3 60.8 1102.6 18.1 100 7.72 314 Colour: 3 Clarity: 1 Clear None 6 62.5 902.8 14.4 100 7.60 331 Colour: 3 Clarity: 2 Clear None 9 41.7 618.5 14.8 100 7.60 336 Colour: 3 Clarity: 2 Clear None 12 50.2 540.8 10.8 97.5 7.60 329 Colour: 3 Clarity: 2 Clear None Table 15: Aqueous product, 50 mg/ml; Storage temp.: -20'C Time- Protein Activity Specific Purity PH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 46.2 780.9 16.9 96.3 7.59 317 Colour: 3 Clarity: 1 Slightly opalescent None 48 1 47.2 899.1 19.0 93.7 7.58 313 Colour: 3 Clarity: 1 Slightly opalescent None 2 53 1222.7 23.1 100.0 7.60 315 Colour: 3 Clarity: 1 Slightly opalescent None 3 61.2 1336.2 21.8 100.0 7.75 320 Colour: 3 Clarity: 1 Slightly opalescent None 6 52.2 1001.3 19.2 100.0 7.60 321 Colour: 3 Clarity: 1 Slightly opalescent None 12 50.4 887.9 17.6 100.0 7.60 320 Colour: 3 Clarity: 1 Slightly opalescent None Table 16: Freeze-dried product, 50 mg/ml; Storage temp.: 5 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Cake/solution Aggregates 0 42.7 759.4 17.8 100.0 7.58 292 Colour: 3 Clarity: 1 49 Cake: yellow, some cracks Solution: Clear None 1 42.6 840.4 19.7 63.1 7.58 293 Colour: 3 Clarity: 1 Cake: yellow, some cracks Solution: Clear None 2 42.1 937.0 22.3 100.0 7.60 292 Colour: 3 Clarity: 1 Cake: yellow, some cracks Solution: Clear None 3 47.4 1014.7 21.4 100.0 7.75 291 Colour: 3 Clarity: 1 Cake: yellow, some cracks Solution: Clear None 6 49.0 876.5 17.9 100.0 7.60 304 Colour: 3 Clarity: 1 Cake: yellow, some cracks Solution: Clear None 12 51.3 945.0 18.4 100.0 7.60 308 Colour: 3 Clarity: 1 Cake: yellow, some cracks Solution: Clear None 50 Table 17: Aqueous product, 100 mg/ml; Storage temp.: 5 0 C Time- Protein Activity Specific Purity PH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 81.8 1705.7 20.9 99.9 7.60 350 Colour: 3 Clarity: 1 Slightly opalescent None 1 85.9 1942.4 22.6 96.9 7.55 352 Colour: 3 Clarity: 1 Slightly opalescent None 2 95.7 1690.8 17.7 96.9 7.65 357 Colour: 3 Clarity: 1 Slightly opalescent None 3 104.3 1671.2 16.0 100.0 7.65 350 Colour: 3 Clarity: 1 Slightly opalescent None 6 96.0 1642.6 17.1 100.0 7.62 360 Colour: 3 Clarity: 1 Slightly opalescent None 9 102.8 1270.8 12.4 100.0 7.63 352 Colour: 3 Clarity: 2 Slightly opalescent None 51 11 86.2 1140.2 13.2 100.0 7.60 353 Colour: 3 Clarity: 2 Slightly opalescent None 12 113.9 1550.6 13.6 100.0 7.58 350 Colour: 3 Clarity: 2 Slightly opalescent None 15 114.7 1160.6 10.1 98.3 7.61 350 Colour: 3 Clarity: 2 Slightly opalescent None 18 86.2 907.4 10.5 100.0 7.67 340 Colour: 3 Clarity: 2 Slightly opalescent None Table 18: Aqueous product, 100 mg/ml; Storage temp.: -20 0 C Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Solution Aggregates 0 81.8 1705.7 20.9 99.9 7.60 316 Colour: 3 Clarity: 1 Slightly opalescent None 1 89.3 2108.8 23.6 100.0 7.56 350 Colour: 3 Clarity: 1 Slightly opalescent None 52 2 112.0 2066.5 18.5 100.0 7.65 353 Colour: 3 Clarity: 1 Slightly opalescent None 3 100.2 2172.4 21.7 96.7 7.65 352 Colour: 3 Clarity: 1 Clear None 6 87.5 2672.3 30.6 100.0 7.62 352 Colour: 3 Clarity: 1 Clear None 9 97.1 2040.3 21.0 100.0 7.62 353 Colour: 3 Clarity: 1 Clear None 11 104.6 2234.0 21.4 100.0 7.60 353 Colour: 3 Clarity: 1 Clear None 12 94.5 1608.8 17.0 100.0 7.57 350 Colour: 3 Clarity: 1 Slightly opalescent None 15 118.0 2015.9 17.1 100.0 7.62 351 Colour: 3 Clarity: 1 Slightly opalescent None 18 90.6 1736.4 19.2 100.0 7.69 338 Colour: 3 Clarity: 1 Slightly opalescent None Table 19: Freeze-dried product, 100 mg/ml; Storage temp.: 5 0

C

53 Time- Protein Activity Specific Purity pH Osmolality Visual point conc. (U/ml) activity (%) (mOsm/kg) observation (month) (mg/ml) (U/mg) Colour 1-3 Clarity 1-3 Cake/solution Aggregates 0 76.0 1638.3 21.5 100.0 7.60 316 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear None 1 71.6 1747.6 24.4 100.0 7.55 318 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear None 2 81.6 1769.9 21.7 100.0 7.63 313 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear None 3 84.1 1616.6 19.2 98.2 7.65 320 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear None 6 96.7 2197.6 22.7 100.0 7.60 324 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear 54 None 9 ND ND ND ND ND ND ND 12 96.0 1978.4 20.6 100.0 7.57 322 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear None 15 ND ND ND ND ND ND ND 18 80.6 1602.6 19.9 100.0 7.75 310 Colour: 3 Clarity: 1 Cake: Yellow, some cracks Solution: Clear None Example 3 Concentrating a rhPBGD composition by tangential flow filtration (TFF) 5 The bulk solution of rhPBGD was then thawed for a minimum of three days at 5 0 C and in darkness. The thawed solution was then centrifuged with 200 mL conical centrifuge tubes for approximately 10 minutes at 2200g. The solution was then filtered through a series of filters with the following pore 10 sizes: 5.0 pm; 0.65 pm; 0.45 pm and 0.20 pm before it was concentrated by tangential flow filtration (TFF). The concentration by TFF was performed with a Millipore Labscale TFF System and Millipore Pellicon@ XL Filter with a pump inlet pressure of approximately 20-25 psi and a pressure over the Pellicon@ XL Filter of approximately 4-6 psi. The rhPBGD 15 was protected from light during the procedure by covering the sample container of the TFF System by sheets of aluminium foil. The concentrated rhPBGD solution obtained from the TFF procedure was then buffer-changed against a formulation buffer containing 3.67 mM Na 2

HPO

4 x 2H 2 0, 55 27 mM glycin and 220 mM Mannitol prepared in sterile water. This was performed by continuously adding said buffer to the TFF-system and pressing it across the membrane until said buffer has replaced the previous buffer. The concentrated and buffer-changed rhPBGD solution was then sterile filtered by 5 passing it through a filter with a pore-size of 0.22 pm. This sterile filtration was performed twice with a new filter each time. The sterile concentrated rhPBGD solution was then placed in vials before it was freeze-dried as described in the method section. 10 Example 4 The effect of different modes of freeze-drying and/or the amount of excipients on the reconstitution time PBGD was concentrated as described in example 3 and after the exchange of the buffer was the concentration of PBGD determined. 15 The concentrated PBGD solution was then freeze-dried in a Lyostar (FTM-systems) freeze-dryer. The solutions were filled in 2 and 6 ml injection glass vials (type 1) and stoppered with rubber stoppers (chlorobutyl). Original freeze-drying cycle: The samples were loaded in ambient temperature and the shelves were cooled 20 down to 04C for 30 minutes. The temperature were lowered to -40 0 C (1 0 C per minute) and held there for 30 minutes and then the vacuum pressure was drawn to 126 mTorr and the primary drying began by raising the temperature to 04C (1 0 C per minute). After 360 minutes of primary drying the temperature was raised to +30 0 C (0.5 4C per minute) and full vacuum was drawn simultaneously 25 (start of secondary drying). The temperature was held at +30 0 C for 360 minutes and the vials were then stoppered under vacuum. Freeze-drying with inclusion of an annealing step: After 30 minutes at -40 0 C the temperature was raised with a rate of 2 0 C per minute to -10 0 C or -20 0 C at which temperature they were kept for 120 or 420 30 minutes before the temperature was lowered again with 2 0 C per minute to -40 0 C were the samples were kept for 60-90 minutes before start of primary drying.

56 The results are shown in Table 20 where the short terms used with regard to the excipients and the freeze-drying cycle mean the following: 1x amount of excipients refers to that the PBGD solution comprises 3.67 mM Na 2

HPO

4 x 2H 2 0, 27 mM glycin and 220 mM Mannitol prepared in sterile water. 5 1.5x amount excipients refers to that the PBGD solution comprises 5.51 mM Na 2

HPO

4 x 2H 2 0, 40.5 mM glycin and 375 mM Mannitol prepared in sterile water, i.e. 1.5x of each of the components present in the 1x buffer. 2x excipients refers to that the PBGD solution comprises 7.34 mM Na 2

HPO

4 x 2H 2 0, 54 mM glycin and 500 mM Mannitol prepared in sterile water, , i.e. 2x of 10 each of the components present in the 1x buffer. The original freeze-drying cycle is as described above. The annealing freeze-drying cycle is as described above where the annealing step comprises raising the temperature to -10 0 C at keeping the sample at this temperature for 120 minutes before lowering it to -40 0 C again. 15 The extended annealing freeze-drying cycle is as described above where the annealing step comprises raising the temperature to -20 0 C at keeping the sample at this temperature for 420 minutes before lowering it to -40 0 C again. Table 20: Amount Protein Reconstitution time for of concentration different free-drying cycles excipients (mg/ml) Original Annealing Extended annealing 1x 198 600 550 480 1x 175 540 500 450 1x 150 450 480 180 1x 125 330 100 10 1x 100 40 10 10 1x 80 25 10 10 1.5x 200 480 40 60 1.5x 175 220 10 10 1.5x 150 60 10 10 1.5x 125 15 10 10 1.5x 100 10 10 10 2x 200 120 20 2x 175 40 20 57 2x 150 20 10 2x 100 10 10 Example 5 The effect of different modes of freeze-drying and/or the amount of excipients on 5 the appearance of the freeze-dried product Concentrated and freeze-dried solutions of PBGD were prepared as described in example 4 and references to the amount of excipients and the type of freeze drying cycle has the same meaning as in example 4. The following results were obtained by visual inspection of the freeze-dried 10 products: A: Comparison of three products prepared from solutions comprising respectively, 4.6 mg/ml 66.6 mg/ml and 109.4 mg/ml rhPBGD showed that the number of cracks in the freeze-dried product increased as concentration of rhPBGD increased. 15 B: Comparison of two products, prepared from a solution comprising 150 mg/ml rhPBGD, and comprising 1x and 1.5x amount of excipients showed that the number of cracks in the freeze-dried product was lower for the product which comprised 1.5x amount of excipients than the product comprising 1x amount of excipients. 20 C: Comparison of two freeze-dried products prepared from a 150 mg/ml rhPBGD solution, comprising 1x and 2x amount of excipients showed that the number of cracks in the freeze-dried product with 2x amount of excipients was lower than the product comprising the 1x amount of excipients. D: Comparison of three freeze-dried products prepared from a 150 mg/ml rhPBGD 25 solution by using the original, the annealing and the extended annealing freeze drying cycle showed that the number of cracks in the freeze-dried product was lower in the product which was prepared according to the annealing freeze-drying cycle than in the product prepared according to the original freeze-drying cycle. Furthermore, the number of cracks in the product prepared according to the 30 extended annealing freeze-drying cycle was lower than in the product prepared according to the annealing freeze-drying cycle.

58 E: Three freeze-dried products were prepared from a 150, 175 and 200 mg/ml, respectively, rhPBGD solution. The freeze-dried products each comprised 1.5x amount of excipients and they were freeze-dried with the annealing cycle. None of the freeze-dried products comprised any cracks. 5 F: Two freeze-dried rhPBGD products were prepared from a 150 mg/ml rhPBGD solution. One of them comprised 1x amount of excipients and was prepared according to the original freeze-drying cycle, while the other comprised 1.5x amount of excipients and was prepared according to the extended annealing free drying cycle. The product comprising 1.5x amount of excipients and prepared 10 according to the extended annealing freeze-drying cycle comprised fewer cracks than the product comprising 1x amount of excipients and prepared according to the original freeze-drying cycle. G: Two freeze-dried rhPBGD products were prepared from a 150 mg/ml rhPBGD solution. One of them comprised 1x amount of excipients and was prepared 15 according to the original freeze-drying cycle, while the other comprised 0.1% Tween 80 in combination with the 1x amount of excipients and was prepared according to the extended annealing freeze-drying cycle. The product comprising the 0.1% Tween 80 in combination with the 1x amount of excipients and which was prepared according to the extended annealing freeze-drying cycle comprised 20 fewer cracks than the product which comprised 1x amount of excipients and which was prepared according to the original freeze-drying cycle. Example 6 The effect of recovery volume, the amount of excipients and the mode of freeze 25 drying on the stability of freeze-dried rhPBGD Concentrated rhPBGD solutions freeze-dried samples were prepared as described in example 4. The "bulk solution" is a concentrated solution of PBGD before freeze-drying. Table 21 shows the results of rhPBGD solutions having the following 30 characteristics with regard to the concentration of rhPBGD, amount of excipients (were the same definitions as in example 4 are used), the mode of freeze-drying (were the same definitions as in example 4 are used) and the ratio of the filling volume (fill. Vol which is the volume of the composition before it is freeze-dried) versus the recovery volume (Rec. vol which is the volume in which the freeze 35 dried product is resuspended): 59 Solution 1: * Approximately 5 mg/ml rhPBGD 1 1x amount of excipient * Original freeze-drying cycle 5 * Fill.vol = Rec. vol Solution 2: * Approximately 70 mg/ml rhPBGD 1 1x amount of excipient * Original freeze-drying cycle 10 * Fill.vol = 2x Rec. vol Solution 3: * Approximately 110 mg/ml rhPBGD 1 1x amount of excipient * Original freeze-drying cycle 15 * Fill.vol = Rec. vol Solution 4: * Approximately 70 mg/ml rhPBGD 1 1x amount of excipient * Original freeze-drying cycle 20 * Fill.vol = 1.5x Rec. vol Solution 5: * Approximately 90 mg/ml rhPBGD * 2/3x amount of excipient * Original freeze-drying cycle 25 * Fill.vol = 1.5x Rec. vol Solution 6: * Approximately 60 mg/ml rhPBGD * 1/2x amount of excipient * Original freeze-drying cycle 30 * Fill.vol = 2x Rec. vol Solution 7: * Approximately 110 mg/ml rhPBGD 1 1x amount of excipient * Annealing freeze-drying cycle 35 * Fill.vol = Rec. vol 60 Solution 8: * Approximately 60 mg/ml rhPBGD 1 1x amount of excipient * Annealing freeze-drying cycle 5 * Fill.vol = 2x Rec. vol Solution 9: * Approximately 150 mg/ml rhPBGD 1 1x amount of excipient * Annealing freeze-drying cycle 10 * Fill.vol = Rec. vol Solution 10: * Approximately 150 mg/ml rhPBGD 1 1x amount of excipient * Original freeze-drying cycle 15 * Fill.vol = Rec. vol Although not shown in Table 21 the purity was also tested for each time point as was found to 100% in all cases. For solution 2 at the week 4 and 9 time point and for solution 4 the week 9 time point a wrong recovery volume was used. 20 61 Table 21: Solution Measurin Fill. Vol Rec. Vol pH Osmolalit Protein Activity Specific g point (ml) (ml) y concentr (U/ml) activity (week) (mosmol/ ation (U/mg) kg) (mg/ml) 1 bulk 4.6 78 17.1 0 0.67 0.67 7.54 274 4.8 85 17.8 2 0.67 0.67 7.22 274 4.6 87 19.4 4 0.67 0.67 7.78 279 5.1 75 14.5 7 0.67 0.67 7.87 284 5.1 68 13.3 9 0.67 0.67 7.67 403 7.0 93 13.2 2 bulk 66.6 1129 16.9 0 0.67 0.335 7.64 525 113 1915 16.9 2 0.67 0.335 7.63 459 93.6 1593 17.0 4 0.67 0.67 7.75 264 64.6 1104 17.1 7 0.67 0.335 7.95 451 106.4 2106 19.8 9 0.67 0.67 7.59 247 51.4 859 16.7 3 bulk 109.4 1491 13.6 0 0.67 0.67 7.75 274 99.9 1598 16.0 2 0.67 0.67 7.64 269 91.4 1543 16.9 4 0.67 0.67 7.68 274 101.2 1825 18.0 7 0.67 0.67 7.71 278 103.4 2045 19.8 9 0.67 0.67 7.67 274 88.3 1656 18.8 4 bulk 71.5 1244 17.4 0 0.67 0.45 7.64 448 113.8 1748 15.4 62 2 0.67 0.45 7.63 411 86.4 1806 20.9 4 0.67 0.45 7.77 362 109.9 1897 17.3 7 0.67 0.45 7.90 379 95.2 686 (7.2) 9 0.67 0.67 7.63 273 59.7 1090 18.3 5 bulk 91.0 1610 17.7 0 0.67 0.45 7.65 296 119.4 2014 16.9 2 0.67 0.45 7.61 285 112.3 2093 18.6 4 0.67 0.45 7.90 292 125.1 2409 19.3 7 0.67 0.45 7.88 297 116.4 1928 16.6 9 0.67 0.45 7.34 278 102.5 1490 14.5 6 bulk 60.7 992 16.3 0 0.67 0.335 7.63 295 112.6 1753 15.6 2 0.67 0.335 7.60 288 86.9 1787 20.6 4 0.67 0.335 7.83 287 116.4 2106 18.1 7 0.67 0.335 8.20 299 109.7 695 (6.3) 9 0.67 0.335 7.44 287 95.2 1636 17.2 7 bulk 116.4 1926 16.5 0 0.67 0.67 7.56 275 101.1 1750 17.3 2 0.67 0.67 7.51 276 93.4 1831 19.6 4 0.67 0.67 7.60 270 101.6 1774 17.5 7 0.67 0.67 7.53 283 102.2 1639 16.0 9 0.67 0.67 7.46 274 89.9 960 10.7 8 bulk 64.5 1119 17.4 0 0.67 0.335 7.52 511 100.7 1718 17.1 2 0.67 0.335 7.51 459 99.3 1900 19.1 63 4 0.67 0.335 7.70 482 114.5 1913 16.7 9 0.67 0.335 7.29 425 102.3 1650 16.1 9 bulk 165 3587 21.7 0 0.60 0.60 7.71 309 121.4 2819 23.2 4 0.60 0.60 7.74 140.3 2014 14.4 7.5 0.60 0.60 7.61 292 135.9 1640 12.1 10 bulk 165 3587 21.7 0 0.60 0.60 7.86 276 142.1 2397 16.9 3 0.40 0.40 8.20 314 141.9 2381 16.8 5 0.60 0.60 7.60 302 131.8 2304 17.5 64 Example 7 Effect of different excipients on the stability of rhPBGD rhPBGD was concentrated as described in example 4 and then the buffer was 5 changed as to one of the four buffers described below. The products were then freeze-dried as described in example 4 with an original annealing step included and the stability of the samples were tested as described in example 6. The effect of the following four formulations on the stability of rhPBGD was tested: Formulation A (corresponds to solution 9 in example 6): 250 mM mannitol, 27 mM 10 glycine and 3.67 mM Na 2

HPO

4 . Formulation B: 250 mM mannitol, 27 mM glycine and 10 mM TRIS-HCL. Formulation C: 250 mM mannitol, 27 mM glycine, 3.67 mM Na 2

HPO

4 and 0.1% Tween 80. Formulation D: 221 mM mannitol, 29 mM sucrose, 27 mM glycine, 3.67 mM 15 Na 2

HPO

4 and 0.1% Tween 80. The results are shown in Table 22.

65 Table 22 Measurin Fill. Vol Rec. Vol pH Osmolalit Protein Activity Formulatio g point (ml) (ml) y concentr (U/ml) n (week) (mosmol/ ation kg) (mg/ml) A Bulk 7.69 366 165 3587 0 0.60 0.60 7.71 309 121.4 2819 4 0.60 0.60 7.74 140.3 2014 7.5 0.60 0.60 7.61 292 135.9 1640 B Bulk 7.54 320 173 3595 0 0.60 0.60 7.58 284 148.1 3726 3 0.60 0.60 7.57 280 165.4 2947 4 0.60 0.60 7.69 167.5 2367 7.5 0.60 0.60 7.60 283 150.4 2235 C Bulk 7.40 338 178 3606 0 0.60 0.60 7.76 290 142.9 2662 3 0.60 0.60 7.43 285 181.7 2332 4 0.60 0.60 7.42 173.1 1436 6 0.60 0.60 7.55 274 156.6 1254 7.5 0.60 0.60 7.34 274 141.5 1252 D Bulk 7.41 337 175 3869 0 0.60 0.60 7.80 292 127.5 2355 3 0.60 0.60 7.35 288 143.9 1988 4 0.60 0.60 7.26 159.3 1644 6 0.60 0.60 7.30 281 135.7 1236 7.5 0.60 0.60 7.28 282 125.7 1146

Claims (11)

1. A method of concentrating a composition comprising porphobilinogen deaminase and/or a functionally equivalent part and analogue hereof, said 5 method comprising: a) centrifugation followed by filtration of a composition comprising said porphobilinogen deaminase; b) concentrating the retentate, obtained from step a); and c) obtaining from the concentrated retenate of step b) an isotonic solution 10 comprising porphobilinogen deaminase, wherein the amount of porphobilinogen deaminase present as aggregates is less than 5 w/w% of the total amount of porphobilinogen deaminase, and wherein the concentration is latest 50 mg/ml of porphobilinogen 15 deaminase andfunctonally equialent parts adanalogues hereof. he method of dain 1, wherein sad porphobiliogen deaminase comprises an amino acid selected from the group consisting of an amno acid sequence as defined by any one of SEQ I NOs: 14 and 20 5; ii. a functionally equialent partof an amino acid sequence as defined Wi ); and WIi a functionally equivalent analogue of an amino acid sequence as defined in i) or li), the amino acid sequence of said analogue being at least 75% 25 identical to an amino acid sequence as defined in i) or ii)

3. The method according to claim 1 or 2, wherein step b) is performed by freeze-drying or evaporation. 30 4 The method according to any one of the preceding cimswrein step b) is performed by ulrafiltration. 5, The method according to claim 4, wherein step b) is performed by tangential flow filtration, 3 f 67

6. The method according to claim 4, wherein step b) is performed with a centrifugal device,

7. The method according to any one of the preceding claims, wherein the o composition comprising porphobilinogen deaminase further comprises one or more of the components selected from the group consisting of: glycine, L serne, sucrose and marnitoi.

8. The method according to any one of the eeng aims, herein the 10 composition comprising porphobi irogen deaminase further comprises one or mor buffers selected from the group consistng of: TRIS-CNa-citrate and PaPIP0 4 , 9 The method according to any one of the preceding clims wherein said 15 method further comprises a step of sterlisation of te concentated composition comprising porphobilinogen deaminase obtained from step b), 10, The method according to any one of the preceding claims, wherein said method further comprises a step of freeze-drying the concentrated composition 20 comprising porphobilinogen deaminase obtained from step b), W. The method according to an one of the preceding ams herein the centrifugation in step a) is performed at 1800-2500 q 25 12, The method according to any one of the preceding aims where the filter used for the filtration in step a has a poresize in the range of 0-.0 to 5,0 micrometer,

13. The method according to any one of the preceding claims, wherein said 30 method further comprises one or more of the following steps prior to step a): i) recombinant expression of porphobilinogen deaminase in a prokaryotic cell or in vertebrate cells in culture ii) purification of porphobilinogen deaminase by one or more steps of chromatography band 35 iii)exchange of he formulation buffer.

14. The method according to clain 3i wherein thecromatography in step ii) is selected from the group consulting of: affinity chromatography, ion exchangecromatography and hydroxyapatite chromatography 5 5 The method according to dairn 13,herein said method comprises the following steps piort step a): ) recombinant expression of pophobilinogen deaminase 5i) subjecting the composition comprising porphobilinogen deaminase from step i) to affinity chromatography; and 10 ii) subjecting the composition comprising porphobilinogen deaminase of step H) to ion exchange chromatography.

16. The method according to claim 13, wherein said method comprises the following steps prior to step a): 15 i) recombinant expression of porphobilinogen deaminase i) subjecting the composition comprising porphobilinogen deaminase from step i) to affinity chromatography; iii) subjecting the composition comprising porphobilinogen deaminase from step li) to ion exchange chromatography; and 20 iv) subjecting the composition comprising porphobilinogen deaminase from step lii) to a hydroxyapatite column.

17. The method according to claim 13, wherein said method comprises recombinant expression using a nucleic acid sequence comprising a sequence 25 selected from the group consisting of: i) a nucleic acid sequence as defined by any one of SEQ ID NOs: 1-13; i) a nucleic acid sequence which is at least 75% identical to a nucleic acid sequence as defined in i), 30 18. The method according to any one of claims 15 or 16, wherein said method further comprises a step of dilution or diafiltration of the composiion comprising porphobilinogen deaminase obtained fom step fi)

19. A position comprising at least 5 mg/mi of porphobilinogen 35 deaminase and functionary equivalent parts and analogues hereof, wherein 69 less than 5% of the total amount of the porphobilinogen deaminase in said composition is present in the form of aggregates,

203. The composition according to claim 19, wherein the porphobilinogen 5 deaminase compares an amino acid selected from the group consisting of: i) an amino acid sequence as defined by anyone of SEQ ID NCs: 14 and 15; ii) a functionally equivalent part of an amino acid sequence as defined in i); and 10 iii) a functional equivalent analogue of an amino acid sequence as defined in I) or ii), the amno acid sequence of said analogue being at least 75% identical to an amino acid sequence as defined in i) or ii). 21. Use of a composition comprising 50-300 mg/ni of porphobilinogen 15 deaminase and functionally equivalent parts and analogues hereof in the manufacture of a medicament for subcutaneous injection into a mammal 22. The use according to claim 21, wherein the porphobilinogen deaminase comprises an amino acid selected from the group consisting of: 20 i) an amino acid sequence as defined by any of SEQ ID NOs: 14 and 15; ii) a functionally equivalent part of an amino acid sequence as defined in i); and iii) a functionally equivalent analogue of an amino acid sequence as defined in i) or ii), the amino acid sequence of said analogue being at least 75% 25 identical to an amino acid sequence as defined in i) or ii). 23. The use according to claim 21 or 22, wherein said polypeptide is porphobilinogen deaminase and wherein the medicament is for the treatment of acute intermittent porphyria. 30 24. A method of treating a mammal for acute intermittent porphyria comprising subcutaneous injection of a composition of 50-300 mg/ml porphobilinogen deaminase, 25. The method according to any one of claims 1-18 substantially as hereinbefore described with reference to any one of the Examples and/or drawings. 26. The composition according to any one of claims 19-20 substantially as hereinbefore described with reference to any one of the Examples and/or drawings. 27, The use according to any one of claims 21-23 substantially as 10 hereinbefore described with reference to any one of the Examples and/or drawings. 28. The method according to claim 24 substantially as hereinbefore described with reference to any one of the Examples and/or drawings. P 39840pc01 1 SEQUENCE LISTING <110> Zymenex A/S Stefan Nilsson 5 <120> A process for concentration of polypeptides <130> 39840pc01 10 <150> PA 2006 00488 <151> 2006-04-04 <150> PA 2006 00922 15 <151> 2006-07-05 <160> 24 <170> FastSEQ for Windows Version 4.0 20 <210> 1 <211> 1035 <212> DNA <213> Homo Sapiens 25 <400> 1 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 30 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaaag 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 35 gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggttgggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 40 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggct 840 accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc acggcaattg 1020 aacgatgccc attaa 1035 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 2 <210> 2 <211> 1035 <212> DNA <213> Homo Sapiens 5 <400> 2 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 10 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaaag 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 15 gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggtggggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 20 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggct 840 accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc acggcaattg 1020 aacgatgccc attaa 1035 25 <210> 3 <211> 1035 <212> DNA <213> Homo Sapiens 30 <400> 3 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 35 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaaag 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 40 gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggtggggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 3 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggct 840 accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc acggcaattg 1020 aacgatgccc attaa 1035 5 <210> 4 <211> 1034 <212> DNA <213> Homo Sapiens 10 <400> 4 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 15 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt cttcacccaa aatttgttgg gaagacccta gaaaccctgc 360 cagagaagag tgtggtggga accagctccc tgcgaagagc agcccagctg cagagaaagt 420 tcccgcatct ggagttcagg agtattcggg gaaacctcaa cacccggctt cggaagctgg 480 20 acgagcagca ggagttcagt gccatcatcc tggcaacagc tggcctgcag cgcatgggct 540 ggcacaaccg ggtggggcag atcctgcacc ctgaggaatg catgtatgct gtgggccagg 600 gggccttggg cgtggaagtg cgagccaagg accaggacat cttggatctg gtgggtgtgc 660 tgcacgatcc cgagactctg cttcgctgca tcgctgaaag ggccttcctg aggcacctgg 720 aaggaggctg cagtgtgcca gtagccgtgc atacagctat gaaggatggg caactgtacc 780 25 tgactggagg agtctggagt ctagacggct cagatagcat acaagagacc atgcaggcta 840 ccatccatgt ccctgcccag catgaagatg gccctgagga tgacccacag ttggtaggca 900 tcactgctcg taacattcca cgagggcccc agttggctgc ccagaacttg ggcatcagcc 960 tggccaactt gttgctgagc aaaggagcca aaaacatcct ggatgttgca cggcaattga 1020 acgatgccca ttaa 1034 30 <210> 5 <211> 1035 <212> DNA <213> Hoo Sapiens 35 <400> 5 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacgggcagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 40 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaagg 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 4 gacgagcagc aggagttcag tgtcatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggttgggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 5 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggct 840 accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caagggagcc aaaaacatcc tggatgttgc acggcaattg 1020 aacgatgccc attaa 1035 10 <210> 6 <211> 1035 <212> DNA <213> Homo Sapiens 15 <400> 6 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 20 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaaag 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 25 gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggtggggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggtt gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 30 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggct 840 accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc acggcaattg 1020 aacgatgccc attaa 1035 35 <210> 7 <211> 1034 <212> DNA <213> Homo Sapiens 40 <400> 7 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 5 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaaag 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 5 gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggtggggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 10 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggct 840 accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc acggcaatta 1020 acgatgccca ttaa 1034 15 <210> 8 <211> 1035 <212> DNA <213> Homo Sapiens 20 <400> 8 atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca gacggacagt 60 gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat tgctatgtcc 120 accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa aagcctgttt 180 25 accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt tcactccttg 240 aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg caagcgggaa 300 aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct agaaaccctg 360 ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct gcagagaaag 420 ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct tcggaagctg 480 30 gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca gcgcatgggc 540 tggcacaacc gggtggggca gatcctgcac cctgaggaat gcatgtatgc tgtgggccag 600 ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct ggtgggtgtg 660 ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct gaggcacctg 720 gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg gcaactgtac 780 35 ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac catgcaggcc 840 accatccatg tccctaccca gcatgaagat ggccctgagg atgacccaca gttggtaggc 900 atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt gggcatcagc 960 ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc acggcaattg 1020 aacgatgccc attaa 1035 40 <210> 9 <211> 1260 <212> DNA <213> Homo Sapiens 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 6 <400> 9 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga 60 acaaaagctg gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct 120 gcaggaattc atgagagtga ttcgcgtggg tacccgcaag agccagcttg ctcgcataca 180 5 gacggacagt gtggtggcaa cattgaaagc ctcgtaccct ggcctgcagt ttgaaatcat 240 tgctatgtcc accacagggg acaagattct tgatactgca ctctctaaga ttggagagaa 300 aagcctgttt accaaggagc ttgaacatgc cctggagaag aatgaagtgg acctggttgt 360 tcactccttg aaggacctgc ccactgtgct tcctcctggc ttcaccatcg gagccatctg 420 caagcgggaa aaccctcatg atgctgttgt ctttcaccca aaatttgttg ggaagaccct 480 10 agaaaccctg ccagagaaga gtgtggtggg aaccagctcc ctgcgaagag cagcccagct 540 gcagagaaag ttcccgcatc tggagttcag gagtattcgg ggaaacctca acacccggct 600 tcggaagctg gacgagcagc aggagttcag tgccatcatc ctggcaacag ctggcctgca 660 gcgcatgggc tggcacaacc gggttgggca gatcctgcac cctgaggaat gcatgtatgc 720 tgtgggccag ggggccttgg gcgtggaagt gcgagccaag gaccaggaca tcttggatct 780 15 ggtgggtgtg ctgcacgatc ccgagactct gcttcgctgc atcgctgaaa gggccttcct 840 gaggcacctg gaaggaggct gcagtgtgcc agtagccgtg catacagcta tgaaggatgg 900 gcaactgtac ctgactggag gagtctggag tctagacggc tcagatagca tacaagagac 960 catgcaggct accatccatg tccctgccca gcatgaagat ggccctgagg atgacccaca 1020 gttggtaggc atcactgctc gtaacattcc acgagggccc cagttggctg cccagaactt 1080 20 gggcatcagc ctggccaact tgttgctgag caaaggagcc aaaaacatcc tggatgttgc 1140 acggcaattg aacgatgccc attaataagc ttatcgatac cgtcgacctc gagggggggc 1200 ccggtaccca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa 1260 25 <210> 10 <211> 1113 <212> DNA <213> Homo Sapiens 30 <400> 10 cacacagcct actttccaag cggagccatg tctggtaacg gcaatgcggc tgcaacggcg 60 gaagaaaaca gcccaaagat gagagtgatt cgcgtgggta cccgcaagag ccagcttgct 120 cgcatacaga cggacagtgt ggtggcaaca ttgaaagcct cgtaccctgg cctgcagttt 180 gaaatcattg ctatgtccac cacaggggac aagattcttg atactgcact ctctaagatt 240 35 ggagagaaaa gcctgtttac caaggagctt gaacatgccc tggagaagaa tgaagtggac 300 ctggttgttc actccttgaa ggacctgccc actgtgcttc ctcctggctt caccatcgga 360 gccatctgca agcgggaaaa ccctcatgat gctgttgtct ttcacccaaa atttgttggg 420 aagaccctag aaaccctgcc agagaagagt gtggtgggaa ccagctccct gcgaagagca 480 gcccagctgc agagaaagtt cccgcatctg gagttcagga gtattcgggg aaacctcaac 540 40 acccggcttc ggaagctgga cgagcagcag gagttcagtg ccatcatcct ggcaacagct 600 ggcctgcagc gcatgggctg gcacaaccgg gttgggcaga tcctgcaccc tgaggaatgc 660 atgtatgctg tgggccaggg ggccttgggc gtggaagtgc gagccaagga ccaggacatc 720 ttggatctgg tgggtgtgct gcacgatccc gagactctgc ttcgctgcat cgctgaaagg 780 gccttcctga ggcacctgga aggaggctgc agtgtgccag tagccgtgca tacagctatg 840 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 7 aaggatgggc aactgtacct gactggagga gtctggagtc tagacggctc agatagcata 900 caagagacca tgcaggctac catccatgtc cctgcccagc atgaagatgg ccctgaggat 960 gacccacagt tggtaggcat cactgctcgt aacattccac gagggcccca gttggctgcc 1020 cagaacttgg gcatcagcct ggccaacttg ttgctgagca aaggagccaa aaacatcctg 1080 gatgttgcac ggcaattgaa cgatgcccat taa 1113 5 <210> 11 <211> 1380 <212> DNA <213> Homo Sapiens 10 <400> 11 agcaggtcct actatcgcct ccctctagtc tctgcttctt tggatccctg aggagggcag 60 aaggaagaaa acagcccaaa gatgagagtg attcgcgtgg gtacccgcaa gagccagctt 120 gctcgcatac agacggacag tgtggtggca acattgaaag cctcgtaccc tggcctgcag 180 15 tttgaaatca ttgctatgtc caccacaggg gacaagattc ttgatactgc actctctaag 240 attggagaga aaagcctgtt taccaaggag cttgaacatg ccctggagaa gaatgaagtg 300 gacctggttg ttcactcctt gaaggacctg cccactgtgc ttcctcctgg cttcaccatc 360 ggagccatct gcaagcggga aaaccctcat gatgctgttg tctttcaccc aaaatttgtt 420 gggaagaccc tagaaaccct gccagagaag agtgtggtgg gaaccagctc cctgcgaaga 480 20 gcagcccagc tgcagagaaa gttcccgcat ctggagttca ggagtattcg gggaaacctc 540 aacacccggc ttcggaagct ggacgagcag caggagttca gtgccatcat cctagcaaca 600 gctggcctgc agcgcatggg ctggcacaac cgggttgggc agatcctgca ccctgaggaa 660 tgcatgtatg ctgtgggcca gggggccttg ggcgtggaag tgcgagccaa ggaccaggac 720 atcttggatc tggtgggtgt gctgcacgat cccgagactc tgcttcgctg catcgctgaa 780 25 agggccttcc tgaggcacct ggaaggaggc tgcagtgtgc cagtagccgt gcatacagct 840 atgaaggatg ggcaactgta cctgactgga ggagtctgga gtctagacgg ctcagatagc 900 atacaagaga ccatgcaggc taccatccat gtccctgccc agcatgaaga tggccctgag 960 gatgacccac agttggtagg catcactgct cgtaacattc cacgagggcc ccagttggct 1020 gcccagaact tgggcatcag cctggccaac ttgttgctga gcaaaggagc caaaaccatc 1080 30 ctggatgttg cacggcagct taacgatgcc cattaactgg tttgtggggc acagatgcct 1140 gggttgctgc tgtccagtgc ctacatcccg ggcctcagtg ccccattctc actgctatct 1200 ggggagtgat taccccggga gactgaactg cagggttcaa gccttccagg gatttgcctc 1260 accttggggc cttgatgact gccttgcctc ctcagtatgt gggggcttca tctctttaga 1320 gaagtccaag caacagcctt tgaatgtaac caatcctact aataaaccag ttctgaaggt 1380 35 <210> 12 <211> 1377 <212> DNA 40 <213> Homo Sapiens <400> 12 cacacagcct actttccaag cggagccatg tctggtaacg gcaatgcggc tgcaacggcg 60 gaagaaaaca gcccaaagat gagagtgatt cgcgtgggta cccgcaagag ccagcttgct 120 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 8 cgcatacaga cggacagtgt ggtggcaaca ttgaaagcct cgtaccctgg cctgcagttt 180 gaaatcattg ctatgtccac cacaggggac aagattcttg atactgcact ctctaagatt 240 ggagagaaaa gcctgtttac caaggagctt gaacatgccc tggagaagaa tgaagtggac 300 ctggttgttc actccttgaa ggacctgccc actgtgcttc ctcctggctt caccatcgga 360 gccatctgca agcgggaaaa ccctcatgat gctgttgtct ttcacccaaa atttgttggg 420 5 aagaccctag aaaccctgcc agagaagagt gtggtgggaa ccagctccct gcgaagagca 480 gcccagctgc agagaaagtt cccgcatctg gagttcagga gtattcgggg aaacctcaac 540 acccggcttc ggaagctgga cgagcagcag gagttcagtg ccatcatcct agcaacagct 600 ggcctgcagc gcatgggctg gcacaaccgg gtggggcaga tcctgcaccc tgagaaatgc 660 atgtatgctg tgggccaggg ggccttgggc gtggaagtgc gagccaagga ccaggacatc 720 10 ttggatctgg tgggtgtgct gcacgatccc gagactctgc ttcgctgcat cgctgaaagg 780 gccttcctga ggcacctgga aggaggctgc agtgtgccag tagccgtgca tacagctatg 840 aaggatgggc aactgtacct gactggagga gtctggagtc tagacggctc agatagcata 900 caagagacca tgcaggctac catccatgtc cctgcccagc atgaagatgg ccctgaggat 960 gacccacagt tggtaggcat cactgctcgt aacattccac gagggcccca gttggctgcc 1020 15 cagaacttgg gcatcagcct ggccaacttg ttgctgagca aaggagccaa aaacatcctg 1080 gatgttgcac ggcagcttaa cgatgcccat taactggttt gtggggcaca gatgcctggg 1140 ttgctgctgt ccagtgccta catcccgggc ctcagtgccc cattctcact gctatctggg 1200 gagtgattac cccgggagac tgaactgcag ggttcaagcc ttccagggat ttgcctcacc 1260 ttggggcctt gatgactgcc ttgcctcctc agtatgtggg ggcttcatct ctttagagaa 1320 20 gtccaagcaa cagcctttga atgtaaccaa tcctactaat aaaccagttc tgaaggt 1377 <210> 13 <211> 10024 <212> DNA 25 <213> Homo Sapiens <400> 13 aatcatgatt gttaattatg ttcatgatta caggcgcggt ggctcacgcc tgtactccca 60 gcactttggg aggccgaggt gggcgaatca cctgaggtca ggagttcaag acctgcctga 120 30 ctaacatgga gaaacctcat ctctaccaaa aatacaaaat tagccgggtg tggtggtgcg 180 tgcctgtaat cccagctact cggggggctg aggcaggaga attgcttgaa cccgggaggc 240 ggaggttgca gtgagctgag atcgtgccat tgcattccag cctgggcaac aagagcgaaa 300 ctccgtctca aaaaaaaaaa aaaattatgt tcatgggaaa gcacttttcc taacaagccc 360 ttttctcact acatgtaggt ttgtgctccc acttcagtta cttgtcttta ggcatgacct 420 35 ttaatctctc tgaaccagtt tcctcatttt aagaattgaa atgctggctg ggccagtcgt 480 cacgcctgta atcccagcac tttgggaggc caaggcgaga tgactgcttg agtccaggag 540 ttcgagacta gcctgggcaa catagtgagg ccacctcccc gctgtctcta taaaaaaatc 600 tagaaattag tcccacgtgg tgatgtgcgc ctgtagtccc agctgcttgg gaggctgagg 660 tggggggatc gctgaagccg ggaggtcaag gctgcagtga cccgtggtca tgccgctgca 720 40 ctctagtctg gggacacagt gagaccccgt atcaaaaaga aaaatgctgc ctatttcaag 780 gttgtagcaa agctaagttt gaacagagca aaggaagcgc catagaagct gcactacttg 840 ctcatgtcac agctggggaa tggggaggtc gaatggggag gtccactgtc gcaatgttcc 900 aattcccgcc cagagggagg gacctcccct tcgagggagg gcgccggaag tgacgcgagg 960 ctctgcggag accaggagtc agactgtagg acgacctcgg gtcccacgtg tccccggtac 1020 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 9 tcgccggccg gagcctccgg cttcccgggg ccgggggacc ttagcggcac ccacacacag 1080 cctactttcc aagcggagcc atgtctggta acggcaatgc ggctgcaacg gcggtgagtg 1140 ctgagccggt gaccagcaca ctttgggctt ctggacgagc cgtgcagcga ttggccccag 1200 gttgccatcc tcagtcgtct attggtcaga acggctatct tttttttttt tttttttttt 1260 tttttggtcc gagtagcttt taaagggcca gtagctcggt tgccctccgg aaggaatggg 1320 5 gaaatcagag agcggtgata ctgggttaag agtggaagga ttgtttggaa cggaactccg 1380 gtccctgcgg gcatctgggt gggattccca tcaggcctgg gatgcacggc tctagattta 1440 gtgacccaga ccaagaacgt tcgtctacac agacggggtc ctttcattcg aggctgggct 1500 gaggcggatg cagatacggc ccctttggga agacacgttc cacttttgat tcataggaga 1560 gagtatcagc caagcctccg aactgcacac aaacgtctta gaagtgcgcc ttctttttgt 1620 10 gttatagtgg tctcccagcc acagccaacg ctccaagtcc ccagctgtga cacacctact 1680 gaattactac cgtgggtggg aggccgccgt gggcctttcc attacgagcc tgcttgccga 1740 gccctgggct tgtgcacaga caaactgcag agctggtgga ggccactgcc aggccgagat 1800 aagaaagaga tggggagctg ctaatctccc cctgtccagc ctgttggtga gggctgggat 1860 ctttgctctt gcagtcattc cagagccctg gactaggagt aggaagatct gaattgtggc 1920 15 cccaactctc tttcggttat tagctctgtg accctaggca agtcacctca tcccttgatg 1980 ccacccgttg cttctgtaac atggtcccaa aggtgcctgt cttgtccacc tgataggatt 2040 tttgagacga caacaatatg caaaagcaat agcttcaaca tagaagtgct cagtgtttta 2100 ttttttaatg aaacggtttg acttggatat gctgtgcaca ttcaatgaac ttaaggaatt 2160 gtttgaacct agtagttctg ggaccttaga gtcctttctg tgggctccct gtggcccaga 2220 20 tttttggtgg ccacgtttaa tatcaagcct agcctaattt gcaaagggtc tcccagggtt 2280 aatttattgg agtgatcaca tggagtagac cagagtctga gggcagaaag ctgtcacctg 2340 cttcggcaat agaggcccca gatgtctggg tgcaaaagaa ctccatagca ccccgaccaa 2400 catggtgaaa ccccgtctct actaaaaata taaaaattag gccgagcaca gtggctcatg 2460 cctgtaatcc tagcactttg ggaggccgag gcaggtggat tgcctgagct caggagttcg 2520 25 agaccagcct agggaacaca gtgaaacccc gtttctacta aaaatacaaa aaattagccg 2580 acgtggtggc atgcgcctgc agtcccagct acttgggagg ctaagacagg agaatcgctt 2640 gaacctggga ggtggaggtt gcactgagcc gagaccgcgc cattgcactc cagcctgggt 2700 gacagagcgc aactccccct caaaaaaaga aaaaaatata tatatatata tatatatata 2760 tacacacata ttttagctgg gcatggtggt gtgcgtctgt agtagtccca gctacttggg 2820 30 aggctgagtc aggagaatcg cttgaacctg gaaggcagtg gttgtagtta gctgagaaca 2880 tgccactgca ctccagcctg ggcaacagag ggagactctg tctcaaaaaa aaaaaaaaaa 2940 aggaactaca taggatgaac atcccagatc agggaatgtt gactgtcgac agtatcagta 3000 tctacagtgg ctactgtctg atgtagaaag aaatgggatc aggctaggcg tggtggctca 3060 cgcctgtaat cccagctctt tgggaggctg gggcaggagg atcacaagtt cgagaccagc 3120 35 ctggccaaca cagtgaaacc ccgtctctac taaaaatgtg aaaattagct gggcatggtg 3180 gaacatgctg tagttccagc ttgaacccag gggtggaggt tgtagtgagc ctagatcacg 3240 ccactgcact ccagcctgag caaaacagtg agactctgtc taaaaaaaaa aaaaaaaaaa 3300 agagaaatgg gacctccgtc ttagactgaa gaattcagtt ctacgtgctt agcagtgaat 3360 acttttgtcc aaggtactct ggcaggagga agaggcgtgt cctcttgagt tcttgacttg 3420 40 ggctctggcc tgttaatatt tccatgttgg tgaaaccaga ggcagcactc taggtgaacg 3480 aactttaggc agcgcagcct cctagtctta tggaacatct gaggcagaag aaacctgagt 3540 ccaacctttt cattttatag atgaacaaac agatcctgat gggacagtgt acccaaggtc 3600 acccagccaa gaggctgagc aggactgtac gtcagatccg tttacctcag tccttaatgc 3660 atgcagtcca gccagattaa gggaccctta atactgtcag ctttccccac tgtgggatct 3720 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 10 tcatcctctt gacttctttt gtagccagac atctgggcct cttgctggag aaggtggcag 3780 cttgctgctc ttagactcta gtctactcca tgtggcatct ggatggcact gaaattttct 3840 caagtgcctt gtctgttgta gataatgaat ctatcctcca gtgactcagc acaggttccc 3900 cagtgtggtc ctggctgccc tgcccctgcc agctgcaggc cccacccttc ctgtggccag 3960 gctgatgggc cttatctctt tacccacctg gctgtgcaca gcactcccac tgacaactgc 4020 5 cttggtcaag gtgggcttca gggctcagtg tcctggttac tgcagcggca gcaacagcag 4080 gtcctactat cgcctccctc tagtctctgc ttctctggat ccctgaggag ggcagaaggt 4140 actgaggaag gttaaaggga ccagccttgg agtatttccc cactctgaga ctcagctggc 4200 cacaggccag gttctgaatt tcctttcttc caagccagtg attctggttc ttggacaagg 4260 tgttgaggaa cactagaaac agaggggact gtgacctggg gactttttct gcaggaagaa 4320 10 aacagcccaa agatgagagt gattcgcgtg ggtacccgca agagccaggt gggtgcagga 4380 gccggggtgg aggaggtttg tcagaacagt tatgatgctc acagcatcac aaattggggg 4440 actcagaggg ttagttccta gtatgaagga gatggggtgg ctgggcgtta agttccccgg 4500 gaaatggcag attacattct atggcaagat catccctagg ctgggaaaat tgttggagtg 4560 cagagggctc ccaagcccct tctcatgccc agatggaaat tccagtccct tcaggatctg 4620 15 cctaacctgt gacagtctaa agagtctgag ccgtggctgg gaagggcagg actaatccaa 4680 atctctaccc gcagcttgct cgcatacaga cggacagtgt ggtggcaaca ttgaaagcct 4740 cgtaccctgg cctgcagttt gaaatcagtg agttttctgg aaaggagtgg aagctaatgg 4800 gaagcccagt accccgagag gagagaacac aacatttctg gctttgccta tagctaaagc 4860 ccgtcccgct gccccgagat tccttctggg ctgctcccag ttctgaaggt gctttcctct 4920 20 gaatacctcc agctctgact acctggatta gcctggcatt taacatcttg agctttgggt 4980 ctttttatga gtgtttctgg tcttcctgct cgattgtata tactcagagg gcaggaacca 5040 gggattatgt gcctctgtcc ccatcatgaa tcgtagcaca gtgctaggct cagtaaatgc 5100 tgatcaataa tgagcacctg attgattgac tctctcctca gttgctatgt ccaccacagg 5160 ggacaagatt cttgatactg cactctctaa ggtaacaaca tcttcctccc cagttcttgt 5220 25 ccccactctt ctttccttcc ctgaagggat tcactcaggc tctttctgtc cggcagattg 5280 gagagaaaag cctgtttacc aaggagcttg aacatgccct ggagaagaat gagtaagtaa 5340 agataggaga gtgtggtgcc ctcccagtct cttgctggga ccctagtatg ctaggtctct 5400 tgctgggacc cggggtgtca gataggctgc tgggcttaaa ccctcagaga ggctgaaggc 5460 agctcatagg tgggtttttt caggcttcag aaaaggagag tgtctggttc tgagccatct 5520 30 ggctgcctgg actgcaagaa tggctggggg agggagggta ggagggagag taggagggag 5580 agtgagagga gagcagtttt catgctcctg agatcttgag aaggtgtgct tcctgaactg 5640 ccctaggctc caccactgaa gtagaggcag gggtgggtgg agaaggggtg aaggctggct 5700 gctcataccc tttctctttg cccccctctc ccatctctat agagtggacc tggttgttca 5760 ctccttgaag gacctgccca ctgtgcttcc tcctggcttc accatcggag ccatctgcaa 5820 35 gtaagagtct tgcaagtaag gggcttgggc aggggtaggc atcatgtgaa cctttgcctt 5880 tccctttggg gcctgaccct ctgcttcagg gttatctcct ctgccctgag gagtgttgac 5940 tggtggcaga aaactcaaga aataccagtg agttggcaat cgagagagaa tagaggtgat 6000 ctgaacttaa atctcttccc tcattctgtg cccttccctc ctcccccagg cgggaaaacc 6060 ctcatgatgc tgttgtcttt cacccaaaat ttgttgggaa gaccctagaa accctgccag 6120 40 agaagaggta agtggggcct ggataggcag cttggtggga tgtgcccaga agatgcaggg 6180 atgggaggag gaggaaagga acagtgactg cctagtgtta aaatctcatt gtaacttctc 6240 tctgggcagt gtggtgggaa ccagctccct gcgaagagca gcccagctgc agagaaagtt 6300 cccgcatctg gagttcagga gtattgtatc cttttagaag agtgacggat ccttttggaa 6360 gagtgacgga gacagcagcc aaggaaaaag acaaggtcta gagggctctg ggagtccgga 6420 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 11 gagtggaagg ggcttccagc aagcagcccg tggggtcagt ggcctgtctg tctttccatg 6480 cactcatccg tccactcatt tacagtctaa tgttttctta gccccagaca agtgttcaga 6540 gtgcaaggca ttggggataa tggtgagcaa gataaacatt cccctgcata tgtagagttt 6600 acgtcttact tagggataat gcagttatac tgaactgaat agtgactact tctggaggga 6660 tagggagtac ttcctttttt tttttttttt tttctgagac ggagtctcgc tctgttgccc 6720 5 aggttggagt gcagtggcgc aatctaggct cactgcaact tctgcctcct gagttcaagc 6780 aatcttcctg cctcagcctc ctaagtagtt gggattacag gtgccaccac acctggctaa 6840 tttttgtatt tttagtagag actgggtttc accatgttag tcaggctggt ctcaaactcc 6900 tgacctcagg tgatccacca gcctcggcct cccaaagggc tgggattaca ggcttgagcc 6960 ccgcacccgg tcagtacttc catttttata tgctactata ttgtcttgac ttttacaatg 7020 10 aatatgtagt acatttcata aaactaaatt taaaaatagt atgtgctaag tgctccaata 7080 agtgaagttg ggaattttct ggaaacttct agttggaaca tctaaacaca gaagtctggg 7140 gtgtcaggga aggtttctca gaggtcttgt aaccttggca agttatttag cctccctatg 7200 tcattttcct tatctgtaaa gtggggataa taatactacc ttcctcacag ggttgttgtg 7260 aagatgaaat gagctgacat atggaaagta cttttagagc agtgtctggc atgtagtaag 7320 15 tatgatgtaa ctgttagctg ttaacattaa gctgagagct ggaagatgac tgaaagtcag 7380 ccagctagag agggaaagac agactcaggc agagggaacc gcacgaggcc ccagattgcc 7440 cgacactgtg gtccttagca actctccaca gcggggaaac ctcaacaccc ggcttcggaa 7500 gatggacgag cagcaggagt tcagtgccat catcctggca acagctggcc tgcagcgcat 7560 gggctggcac aaccgggttg ggcaggtagg gcctgcccct atcctctccc cagctcatct 7620 20 gcatctcctt tctgccttac agtcatcccc aatttaggat ttttagactt tatgattgtg 7680 tgaaagcgat atacgttcag tagaaactgt acttagtacc catacagcca ttctgttttt 7740 tactttcagt acagtattca ttacatgaga tattcacttt attgtaaaac aggcttggtg 7800 tcagatgatt ttgtccaact ataataggct aatcttaagt gttctgagca catgtaaggt 7860 aggctaggtg tattaaatgc attttcagct tgttttcaac ttaacaatgg gtttatcagg 7920 25 atgtaaccct attgtaagtc aaggaccatc tgtcttcact tcttgaccac cccacctcta 7980 acaccgtagg ctgggaagat tgtgaatcag aggccagact ctaggctttc atggagaaaa 8040 tttacaaaaa aaaaaaaaag aggccagact cacacttagg cctacccagg ctttctagat 8100 gatagggaac tcccatctca ctgccaggtg cttttagaca cccccgtgtc cacccttttg 8160 actccctgtt ccgcctccac agatcctgca ccctgaggaa tgcatgtatg ctgtgggcca 8220 30 ggtacacttg accagggaag ccacatggtg acatatgcct tccctttgtt ctcaaccaag 8280 aagcttgtct cacaaccttc tgcatctgct tccccagaat agcattctca gggaggggca 8340 gaccttggga tgctaccggt ccaaaaggcg ctggggagca agtagataga ggtggtccca 8400 tgctttgcgc cattggttgg ggaaagatca ggcctgatgt cctaggatgt ttttccatca 8460 gggggccttg ggcgtggaag tgcgagccaa ggaccaggac atcttggatc tggtgggtgt 8520 35 gctgcacgat cccgagactc tgcttcgctg catcgctgaa agggccttcc tgaggcacct 8580 ggtagggcct gtgctccacc tgtggagggc tggggacttg gagagctggg aaaggtggca 8640 gggaagattt cttacatgaa tgctctgtat acagtgctaa ctcattcttg ttgaatgttg 8700 tgtatggata ggaccaggtc tgggcccaca gttgcctttt cagtgatgtc ctcaggtctg 8760 tggtcacagg gtggtgttaa gagcccttgc agctcacaag aacttcttgt tacaggaagg 8820 40 aggctgcagt gtgccagtag ccgtgcatac agctatgaag gatgggcaag taagtggggg 8880 gaaatgggcg ggaagccagg gaaaggagga ctgtggcatt tcttcctgtg catcccaggt 8940 ttctaggtag tcccctctca gactgtgctg aggcaactgt tttcttcccc agctgtacct 9000 gactggagga gtctggagtc tagacggctc agatagcata caagagacca tgcaggctac 9060 catccatgtc cctgcccagg taccaaagct ggagggcgag ggggtaataa acaagagtgc 9120 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 12 atataatctc ttgttctcac caaatcccac ctccttccct catacagcat gaagatggcc 9180 ctgaggatga cccacagttg gtaggcatca ctgctcgtaa cattccacga gggccccagt 9240 tggctgccca gaacttgggc atcagcctgg ccaacttgtt gctgagcaaa ggagccaaaa 9300 acatcctgga tgttgcacgg cagcttaacg atgcccatta actggtttgt ggggcacaga 9360 tgcctgggtt gctgctgtcc agtgcctaca tcccgggcct cagtgcccca ttctcactgc 9420 5 tatctgggga gtgattaccc cgggagactg aactgcaggg ttcaagcctt ccagggattt 9480 gcctcacctt ggggccttga tgactgcctt gcctcctcag tatgtggggg cttcatctct 9540 ttagagaagt ccaagcaaca gcctttgaat gtaaccaatc ctactaataa accagttctg 9600 aaggtgttgt gtgtgcgcgt gtggagttgg cgggaagata ggaacaaaca caaagccctt 9660 tcatccttac ctcagaggct gggacttttg cccagagttc tcctggtacg tcctttctgc 9720 10 ttctgcctca atagttttca tttcacacag aataaattgt ctcccaggaa caccaagaaa 9780 cagagccaca atcttaaatt cctatggttt gccccttcag ttaacagtag agcctgttta 9840 tattgcatgg cccctcccac ccctattatc aggaaagtat agaaagtcac taattctaca 9900 actctcttgc aaaatgaaaa caaatgctcc atttaaaaaa aaaacaatcc tttaataaaa 9960 ttagtccatc taaaactccc caatgcctaa ggttctagtc gtggaagggt tagctgcaga 10020 15 attc 10024 <210> 14 20 <211> 361 <212> PRT <213> Homo Sapiens <400> 14 25 Met Ser Gly Asn Gly Asn Ala Ala Ala Thr Ala Glu Glu Asn Ser Pro 1 5 10 15 Lys Met Arg Val Ile Arg Val Gly Thr Arg Lys Ser Gln Leu Ala Arg 20 25 30 Ile Gln Thr Asp Ser Val Val Ala Thr Leu Lys Ala Ser Tyr Pro Gly 30 35 40 45 Leu Gln Phe Glu Ile Ile Ala Met Ser Thr Thr Gly Asp Lys Ile Leu 50 55 60 Asp Thr Ala Leu Ser Lys Ile Gly Glu Lys Ser Leu Phe Thr Lys Glu 65 70 75 80 35 Leu Glu His Ala Leu Glu Lys Asn Glu Val Asp Leu Val Val His Ser 85 90 95 Leu Lys Asp Leu Pro Thr Val Leu Pro Pro Gly Phe Thr Ile Gly Ala 100 105 110 Ile Cys Lys Arg Glu Asn Pro His Asp Ala Val Val Phe His Pro Lys 40 115 120 125 Phe Val Gly Lys Thr Leu Glu Thr Leu Pro Glu Lys Ser Val Val Gly 130 135 140 Thr Ser Ser Leu Arg Arg Ala Ala Gln Leu Gln Arg Lys Phe Pro His 145 150 155 160 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 13 Leu Glu Phe Arg Ser Ile Arg Gly Asn Leu Asn Thr Arg Leu Arg Lys 165 170 175 Met Asp Glu Gln Gln Glu Phe Ser Ala Ile Ile Leu Ala Thr Ala Gly 180 185 190 Leu Gln Arg Met Gly Trp His Asn Arg Val Gly Gln Ile Leu His Pro 5 195 200 205 Glu Glu Cys Met Tyr Ala Val Gly Gln Gly Ala Leu Gly Val Glu Val 210 215 220 Arg Ala Lys Asp Gln Asp Ile Leu Asp Leu Val Gly Val Leu His Asp 225 230 235 240 10 Pro Glu Thr Leu Leu Arg Cys Ile Ala Glu Arg Ala Phe Leu Arg His 245 250 255 Leu Glu Gly Gly Cys Ser Val Pro Val Ala Val His Thr Ala Met Lys 260 265 270 Asp Gly Gln Leu Tyr Leu Thr Gly Gly Val Trp Ser Leu Asp Gly Ser 15 275 280 285 Asp Ser Ile Gln Glu Thr Met Gln Ala Thr Ile His Val Pro Ala Gln 290 295 300 His Glu Asp Gly Pro Glu Asp Asp Pro Gln Leu Val Gly Ile Thr Ala 305 310 315 320 20 Arg Asn Ile Pro Arg Gly Pro Gln Leu Ala Ala Gln Asn Leu Gly Ile 325 330 335 Ser Leu Ala Asn Leu Leu Leu Ser Lys Gly Ala Lys Asn Ile Leu Asp 340 345 350 Val Ala Arg Gln Leu Asn Asp Ala His 25 355 360 <210> 15 <211> 344 30 <212> PRT <213> Homo Sapiens <400> 15 Met Arg Val Ile Arg Val Gly Thr Arg Lys Ser Gln Leu Ala Arg Ile 35 1 5 10 15 Gln Thr Asp Ser Val Val Ala Thr Leu Lys Ala Ser Tyr Pro Gly Leu 20 25 30 Gln Phe Glu Ile Ile Ala Met Ser Thr Thr Gly Asp Lys Ile Leu Asp 35 40 45 40 Thr Ala Leu Ser Lys Ile Gly Glu Lys Ser Leu Phe Thr Lys Glu Leu 50 55 60 Glu His Ala Leu Glu Lys Asn Glu Val Asp Leu Val Val His Ser Leu 65 70 75 80 Lys Asp Leu Pro Thr Val Leu Pro Pro Gly Phe Thr Ile Gly Ala Ile 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 14 85 90 95 Cys Lys Arg Glu Asn Pro His Asp Ala Val Val Phe His Pro Lys Phe 100 105 110 Val Gly Lys Thr Leu Glu Thr Leu Pro Glu Lys Ser Val Val Gly Thr 115 120 125 5 Ser Ser Leu Arg Arg Ala Ala Gln Leu Gln Arg Lys Phe Pro His Leu 130 135 140 Glu Phe Arg Ser Ile Arg Gly Asn Leu Asn Thr Arg Leu Arg Lys Met 145 150 155 160 Asp Glu Gln Gln Glu Phe Ser Ala Ile Ile Leu Ala Thr Ala Gly Leu 10 165 170 175 Gln Arg Met Gly Trp His Asn Arg Val Gly Gln Ile Leu His Pro Glu 180 185 190 Glu Cys Met Tyr Ala Val Gly Gln Gly Ala Leu Gly Val Glu Val Arg 195 200 205 15 Ala Lys Asp Gln Asp Ile Leu Asp Leu Val Gly Val Leu His Asp Pro 210 215 220 Glu Thr Leu Leu Arg Cys Ile Ala Glu Arg Ala Phe Leu Arg His Leu 225 230 235 240 Glu Gly Gly Cys Ser Val Pro Val Ala Val His Thr Ala Met Lys Asp 20 245 250 255 Gly Gln Leu Tyr Leu Thr Gly Gly Val Trp Ser Leu Asp Gly Ser Asp 260 265 270 Ser Ile Gln Glu Thr Met Gln Ala Thr Ile His Val Pro Ala Gln His 275 280 285 25 Glu Asp Gly Pro Glu Asp Asp Pro Gln Leu Val Gly Ile Thr Ala Arg 290 295 300 Asn Ile Pro Arg Gly Pro Gln Leu Ala Ala Gln Asn Leu Gly Ile Ser 305 310 315 320 Leu Ala Asn Leu Leu Leu Ser Lys Gly Ala Lys Asn Ile Leu Asp Val 30 325 330 335 Ala Arg Gln Leu Asn Asp Ala His 340 35 <210> 16 <211> 2022 <212> DNA <213> Homo Sapiens 40 <400> 16 ccggtaccgg ctcctcctgg gctccctcta gcgccttccc cccggcccga ctgcctggtc 60 agcgccaagt gacttacgcc cccgaccctg agcccggacc gctaggcgag gaggatcaga 120 tctccgctcg agaatctgaa ggtgccctgg tcctggagga gttccgtccc agccctgcgg 180 tctcccggta ctgctcgccc cggccctctg gagcttcagg aggcggccgt cagggtcggg 240 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 15 gagtatttgg gtccggggtc tcagggaagg gcggcgcctg ggtctgcggt atcggaaaga 300 gcctgctgga gccaagtagc cctccctctc ttgggacaga cccctcggtc ccatgtccat 360 gggggcaccg cggtccctcc tcctggccct ggctgctggc ctggccgttg cccgtccgcc 420 caacatcgtg ctgatctttg ccgacgacct cggctatggg gacctgggct gctatgggca 480 ccccagctct accactccca acctggacca gctggcggcg ggagggctgc ggttcacaga 540 5 cttctacgtg cctgtgtctc tgtgcacacc ctctagggcc gccctcctga ccggccggct 600 cccggttcgg atgggcatgt accctggcgt cctggtgccc agctcccggg ggggcctgcc 660 cctggaggag gtgaccgtgg ccgaagtcct ggctgcccga ggctacctca caggaatggc 720 cggcaagtgg caccttgggg tggggcctga gggggccttc ctgccccccc atcagggctt 780 ccatcgattt ctaggcatcc cgtactccca cgaccagggc ccctgccaga acctgacctg 840 10 cttcccgccg gccactcctt gcgacggtgg ctgtgaccag ggcctggtcc ccatcccact 900 gttggccaac ctgtccgtgg aggcgcagcc cccctggctg cccggactag aggcccgcta 960 catggctttc gcccatgacc tcatggccga cgcccagcgc caggatcgcc ccttcttcct 1020 gtactatgcc tctcaccaca cccactaccc tcagttcagt gggcagagct ttgcagagcg 1080 ttcaggccgc gggccatttg gggactccct gatggagctg gatgcagctg tggggaccct 1140 15 gatgacagcc ataggggacc tggggctgct tgaagagacg ctggtcatct tcactgcaga 1200 caatggacct gagaccatgc gtatgtcccg aggcggctgc tccggtctct tgcggtgtgg 1260 aaagggaacg acctacgagg gcggtgtccg agagcctgcc ttggccttct ggccaggtca 1320 tatcgctccc ggcgtgaccc acgagctggc cagctccctg gacctgctgc ctaccctggc 1380 agccctggct ggggccccac tgcccaatgt caccttggat ggctttgacc tcagccccct 1440 20 gctgctgggc acaggcaaga gccctcggca gtctctcttc ttctacccgt cctacccaga 1500 cgaggtccgt ggggtttttg ctgtgcggac tggaaagtac aaggctcact tcttcaccca 1560 gggctctgcc cacagtgata ccactgcaga ccctgcctgc cacgcctcca gctctctgac 1620 tgctcatgag cccccgctgc tctatgacct gtccaaggac cctggtgaga actacaacct 1680 gctggggggt gtggccgggg ccaccccaga ggtgctgcaa gccctgaaac agcttcagct 1740 25 gctcaaggcc cagttagacg cagctgtgac cttcggcccc agccaggtgg cccggggcga 1800 ggaccccgcc ctgcagatct gctgtcatcc tggctgcacc ccccgcccag cttgctgcca 1860 ttgcccagat ccccatgcct gagggcccct cggctggcct gggcatgtga tggctcctca 1920 ctgggagcct gtgggggagg ctcaggtgtc tggagggggt ttgtgcctga taacgtaata 1980 acaccagtgg agacttgcac atctgaaaaa aaaaaaaaaa aa 2022 30 <210> 17 <211> 1524 <212> DNA <213> Homo Sapiens 35 <400> 17 atgggggcac cgcggtccct cctcctggcc ctggctgctg gcctggccgt tgcacgtccg 60 cccaacatcg tgctgatctt tgccgacgac ctcggctatg gggacctggg ctgctatggg 120 caccccagct ctaccactcc caacctggac cagctggcgg cgggagggct gcggttcaca 180 40 gacttctacg tgcctgtgtc tctgtgcaca ccctctaggg ccgccctcct gaccggccgg 240 ctcccggttc ggatgggcat gtaccctggc gtcctggtgc ccagctcccg ggggggcctg 300 cccctggagg aggtgaccgt ggccgaagtc ctggctgccc gaggctacct cacaggaatg 360 gccggcaagt ggcaccttgg ggtggggcct gagggggcct tcctgccccc ccatcagggc 420 ttccatcgat ttctaggcat cccgtactcc cacgaccagg gcccctgcca gaacctgacc 480 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 16 tgcttcccgc cggccactcc ttgcgacggt ggctgtgacc agggcctggt ccccatccca 540 ctgttggcca acctgtccgt ggaggcgcag cccccctggc tgcccggact agaggcccgc 600 tacatggctt tcgcccatga cctcatggcc gacgcccagc gccaggatcg ccccttcttc 660 ctgtactatg cctctcacca cacccactac cctcagttca gtgggcagag ctttgcagag 720 cgttcaggcc gcgggccatt tggggactcc ctgatggagc tggatgcagc tgtggggacc 780 5 ctgatgacag ccatagggga cctggggctg cttgaagaga cgctggtcat cttcactgca 840 gacaatggac ctgagaccat gcgtatgtcc cgaggcggct gctccggtct cttgcggtgt 900 ggaaagggaa cgacctacga gggcggtgtc cgagagcctg ccttggcctt ctggccaggt 960 catatcgctc ccggcgtgac ccacgagctg gccagctccc tggacctgct gcctaccctg 1020 gcagccctgg ctggggcccc actgcccaat gtcaccttgg atggctttga cctcagcccc 1080 10 ctgctgctgg gcacaggcaa gagccctcgg cagtctctct tcttctaccc gtcctaccca 1140 gacgaggtcc gtggggtttt tgctgtgcgg actggaaagt acaaggctca cttcttcacc 1200 cagggctctg cccacagtga taccactgca gaccctgcct gccacgcctc cagctctctg 1260 actgctcatg agcccccgct gctctatgac ctgtccaagg accctggtga gaactacaac 1320 ctgctggggg gtgtggccgg ggccacccca gaggtgctgc aagccctgaa acagcttcag 1380 15 ctgctcaagg cccagttaga cgcagctgtg accttcggcc ccagccaggt ggcccggggc 1440 gaggaccccg ccctgcagat ctgctgtcat cctggctgca ccccccgccc agcttgctgc 1500 cattgcccag atccccatgc ctga 1524 <210> 18 20 <211> 507 <212> PRT <213> homo Sapiens <400> 18 25 Met Gly Ala Pro Arg Ser Leu Leu Leu Ala Leu Ala Ala Gly Leu Ala 1 5 10 15 Val Ala Arg Pro Pro Asn Ile Val Leu Ile Phe Ala Asp Asp Leu Gly 20 25 30 Tyr Gly Asp Leu Gly Cys Tyr Gly His Pro Ser Ser Thr Thr Pro Asn 30 35 40 45 Leu Asp Gln Leu Ala Ala Gly Gly Leu Arg Phe Thr Asp Phe Tyr Val 50 55 60 Pro Val Ser Leu Cys Thr Pro Ser Arg Ala Ala Leu Leu Thr Gly Arg 65 70 75 80 35 Leu Pro Val Arg Met Gly Met Tyr Pro Gly Val Leu Val Pro Ser Ser 85 90 95 Arg Gly Gly Leu Pro Leu Glu Glu Val Thr Val Ala Glu Val Leu Ala 100 105 110 Ala Arg Gly Tyr Leu Thr Gly Met Ala Gly Lys Trp His Leu Gly Val 40 115 120 125 Gly Pro Glu Gly Ala Phe Leu Pro Pro His Gln Gly Phe His Arg Phe 130 135 140 Leu Gly Ile Pro Tyr Ser His Asp Gln Gly Pro Cys Gln Asn Leu Thr 145 150 155 160 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 17 Cys Phe Pro Pro Ala Thr Pro Cys Asp Gly Gly Cys Asp Gln Gly Leu 165 170 175 Val Pro Ile Pro Leu Leu Ala Asn Leu Ser Val Glu Ala Gln Pro Pro 180 185 190 Trp Leu Pro Gly Leu Glu Ala Arg Tyr Met Ala Phe Ala His Asp Leu 5 195 200 205 Met Ala Asp Ala Gln Arg Gln Asp Arg Pro Phe Phe Leu Tyr Tyr Ala 210 215 220 Ser His His Thr His Tyr Pro Gln Phe Ser Gly Gln Ser Phe Ala Glu 225 230 235 240 10 Arg Ser Gly Arg Gly Pro Phe Gly Asp Ser Leu Met Glu Leu Asp Ala 245 250 255 Ala Val Gly Thr Leu Met Thr Ala Ile Gly Asp Leu Gly Leu Leu Glu 260 265 270 Glu Thr Leu Val Ile Phe Thr Ala Asp Asn Gly Pro Glu Thr Met Arg 15 275 280 285 Met Ser Arg Gly Gly Cys Ser Gly Leu Leu Arg Cys Gly Lys Gly Thr 290 295 300 Thr Tyr Glu Gly Gly Val Arg Glu Pro Ala Leu Ala Phe Trp Pro Gly 305 310 315 320 20 His Ile Ala Pro Gly Val Thr His Glu Leu Ala Ser Ser Leu Asp Leu 325 330 335 Leu Pro Thr Leu Ala Ala Leu Ala Gly Ala Pro Leu Pro Asn Val Thr 340 345 350 Leu Asp Gly Phe Asp Leu Ser Pro Leu Leu Leu Gly Thr Gly Lys Ser 25 355 360 365 Pro Arg Gln Ser Leu Phe Phe Tyr Pro Ser Tyr Pro Asp Glu Val Arg 370 375 380 Gly Val Phe Ala Val Arg Thr Gly Lys Tyr Lys Ala His Phe Phe Thr 385 390 395 400 30 Gln Gly Ser Ala His Ser Asp Thr Thr Ala Asp Pro Ala Cys His Ala 405 410 415 Ser Ser Ser Leu Thr Ala His Glu Pro Pro Leu Leu Tyr Asp Leu Ser 420 425 430 Lys Asp Pro Gly Glu Asn Tyr Asn Leu Leu Gly Gly Val Ala Gly Ala 35 435 440 445 Thr Pro Glu Val Leu Gln Ala Leu Lys Gln Leu Gln Leu Leu Lys Ala 450 455 460 Gln Leu Asp Ala Ala Val Thr Phe Gly Pro Ser Gln Val Ala Arg Gly 465 470 475 480 40 Glu Asp Pro Ala Leu Gln Ile Cys Cys His Pro Gly Cys Thr Pro Arg 485 490 495 Pro Ala Cys Cys His Cys Pro Asp Pro His Ala 500 505 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 18 <210> 19 <211> 489 <212> PRT <213> Homo Sapiens 5 <220> <221> FORMYLATION <222> 51 <223> C-alpha Formylglycine 10 <400> 19 Arg Pro Pro Asn Ile Val Leu Ile Phe Ala Asp Asp Leu Gly Tyr Gly 1 5 10 15 Asp Leu Gly Cys Tyr Gly His Pro Ser Ser Thr Thr Pro Asn Leu Asp 15 20 25 30 Gln Leu Ala Ala Gly Gly Leu Arg Phe Thr Asp Phe Tyr Val Pro Val 35 40 45 Ser Leu Xaa Thr Pro Ser Arg Ala Ala Leu Leu Thr Gly Arg Leu Pro 50 55 60 20 Val Arg Met Gly Met Tyr Pro Gly Val Leu Val Pro Ser Ser Arg Gly 65 70 75 80 Gly Leu Pro Leu Glu Glu Val Thr Val Ala Glu Val Leu Ala Ala Arg 85 90 95 Gly Tyr Leu Thr Gly Met Ala Gly Lys Trp His Leu Gly Val Gly Pro 25 100 105 110 Glu Gly Ala Phe Leu Pro Pro His Gln Gly Phe His Arg Phe Leu Gly 115 120 125 Ile Pro Tyr Ser His Asp Gln Gly Pro Cys Gln Asn Leu Thr Cys Phe 130 135 140 30 Pro Pro Ala Thr Pro Cys Asp Gly Gly Cys Asp Gln Gly Leu Val Pro 145 150 155 160 Ile Pro Leu Leu Ala Asn Leu Ser Val Glu Ala Gln Pro Pro Trp Leu 165 170 175 Pro Gly Leu Glu Ala Arg Tyr Met Ala Phe Ala His Asp Leu Met Ala 35 180 185 190 Asp Ala Gln Arg Gln Asp Arg Pro Phe Phe Leu Tyr Tyr Ala Ser His 195 200 205 His Thr His Tyr Pro Gln Phe Ser Gly Gln Ser Phe Ala Glu Arg Ser 210 215 220 40 Gly Arg Gly Pro Phe Gly Asp Ser Leu Met Glu Leu Asp Ala Ala Val 225 230 235 240 Gly Thr Leu Met Thr Ala Ile Gly Asp Leu Gly Leu Leu Glu Glu Thr 245 250 255 Leu Val Ile Phe Thr Ala Asp Asn Gly Pro Glu Thr Met Arg Met Ser 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 19 260 265 270 Arg Gly Gly Cys Ser Gly Leu Leu Arg Cys Gly Lys Gly Thr Thr Tyr 275 280 285 Glu Gly Gly Val Arg Glu Pro Ala Leu Ala Phe Trp Pro Gly His Ile 290 295 300 5 Ala Pro Gly Val Thr His Glu Leu Ala Ser Ser Leu Asp Leu Leu Pro 305 310 315 320 Thr Leu Ala Ala Leu Ala Gly Ala Pro Leu Pro Asn Val Thr Leu Asp 325 330 335 Gly Phe Asp Leu Ser Pro Leu Leu Leu Gly Thr Gly Lys Ser Pro Arg 10 340 345 350 Gln Ser Leu Phe Phe Tyr Pro Ser Tyr Pro Asp Glu Val Arg Gly Val 355 360 365 Phe Ala Val Arg Thr Gly Lys Tyr Lys Ala His Phe Phe Thr Gln Gly 370 375 380 15 Ser Ala His Ser Asp Thr Thr Ala Asp Pro Ala Cys His Ala Ser Ser 385 390 395 400 Ser Leu Thr Ala His Glu Pro Pro Leu Leu Tyr Asp Leu Ser Lys Asp 405 410 415 Pro Gly Glu Asn Tyr Asn Leu Leu Gly Gly Val Ala Gly Ala Thr Pro 20 420 425 430 Glu Val Leu Gln Ala Leu Lys Gln Leu Gln Leu Leu Lys Ala Gln Leu 435 440 445 Asp Ala Ala Val Thr Phe Gly Pro Ser Gln Val Ala Arg Gly Glu Asp 450 455 460 25 Pro Ala Leu Gln Ile Cys Cys His Pro Gly Cys Thr Pro Arg Pro Ala 465 470 475 480 Cys Cys His Cys Pro Asp Pro His Ala 485 30 <210> 20 <211> 489 <212> PRT <213> Homo Sapiens 35 <400> 20 Arg Pro Pro Asn Ile Val Leu Ile Phe Ala Asp Asp Leu Gly Tyr Gly 1 5 10 15 Asp Leu Gly Cys Tyr Gly His Pro Ser Ser Thr Thr Pro Asn Leu Asp 40 20 25 30 Gln Leu Ala Ala Gly Gly Leu Arg Phe Thr Asp Phe Tyr Val Pro Val 35 40 45 Ser Leu Cys Thr Pro Ser Arg Ala Ala Leu Leu Thr Gly Arg Leu Pro 50 55 60 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 20 Val Arg Met Gly Met Tyr Pro Gly Val Leu Val Pro Ser Ser Arg Gly 65 70 75 80 Gly Leu Pro Leu Glu Glu Val Thr Val Ala Glu Val Leu Ala Ala Arg 85 90 95 Gly Tyr Leu Thr Gly Met Ala Gly Lys Trp His Leu Gly Val Gly Pro 5 100 105 110 Glu Gly Ala Phe Leu Pro Pro His Gln Gly Phe His Arg Phe Leu Gly 115 120 125 Ile Pro Tyr Ser His Asp Gln Gly Pro Cys Gln Asn Leu Thr Cys Phe 130 135 140 10 Pro Pro Ala Thr Pro Cys Asp Gly Gly Cys Asp Gln Gly Leu Val Pro 145 150 155 160 Ile Pro Leu Leu Ala Asn Leu Ser Val Glu Ala Gln Pro Pro Trp Leu 165 170 175 Pro Gly Leu Glu Ala Arg Tyr Met Ala Phe Ala His Asp Leu Met Ala 15 180 185 190 Asp Ala Gln Arg Gln Asp Arg Pro Phe Phe Leu Tyr Tyr Ala Ser His 195 200 205 His Thr His Tyr Pro Gln Phe Ser Gly Gln Ser Phe Ala Glu Arg Ser 210 215 220 20 Gly Arg Gly Pro Phe Gly Asp Ser Leu Met Glu Leu Asp Ala Ala Val 225 230 235 240 Gly Thr Leu Met Thr Ala Ile Gly Asp Leu Gly Leu Leu Glu Glu Thr 245 250 255 Leu Val Ile Phe Thr Ala Asp Asn Gly Pro Glu Thr Met Arg Met Ser 25 260 265 270 Arg Gly Gly Cys Ser Gly Leu Leu Arg Cys Gly Lys Gly Thr Thr Tyr 275 280 285 Glu Gly Gly Val Arg Glu Pro Ala Leu Ala Phe Trp Pro Gly His Ile 290 295 300 30 Ala Pro Gly Val Thr His Glu Leu Ala Ser Ser Leu Asp Leu Leu Pro 305 310 315 320 Thr Leu Ala Ala Leu Ala Gly Ala Pro Leu Pro Asn Val Thr Leu Asp 325 330 335 Gly Phe Asp Leu Ser Pro Leu Leu Leu Gly Thr Gly Lys Ser Pro Arg 35 340 345 350 Gln Ser Leu Phe Phe Tyr Pro Ser Tyr Pro Asp Glu Val Arg Gly Val 355 360 365 Phe Ala Val Arg Thr Gly Lys Tyr Lys Ala His Phe Phe Thr Gln Gly 370 375 380 40 Ser Ala His Ser Asp Thr Thr Ala Asp Pro Ala Cys His Ala Ser Ser 385 390 395 400 Ser Leu Thr Ala His Glu Pro Pro Leu Leu Tyr Asp Leu Ser Lys Asp 405 410 415 Pro Gly Glu Asn Tyr Asn Leu Leu Gly Gly Val Ala Gly Ala Thr Pro 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 21 420 425 430 Glu Val Leu Gln Ala Leu Lys Gln Leu Gln Leu Leu Lys Ala Gln Leu 435 440 445 Asp Ala Ala Val Thr Phe Gly Pro Ser Gln Val Ala Arg Gly Glu Asp 450 455 460 5 Pro Ala Leu Gln Ile Cys Cys His Pro Gly Cys Thr Pro Arg Pro Ala 465 470 475 480 Cys Cys His Cys Pro Asp Pro His Ala 485 10 <210> 21 <211> 1011 <212> PRT <213> Homo Sapiens 15 <400> 21 Met Gly Ala Tyr Ala Arg Ala Ser Gly Val Cys Ala Arg Gly Cys Leu 1 5 10 15 Asp Ser Ala Gly Pro Trp Thr Met Ser Arg Ala Leu Arg Pro Pro Leu 20 20 25 30 Pro Pro Leu Cys Phe Phe Leu Leu Leu Leu Ala Ala Ala Gly Ala Arg 35 40 45 Ala Gly Gly Tyr Glu Thr Cys Pro Thr Val Gln Pro Asn Met Leu Asn 50 55 60 25 Val His Leu Leu Pro His Thr His Asp Asp Val Gly Trp Leu Lys Thr 65 70 75 80 Val Asp Gln Tyr Phe Tyr Gly Ile Lys Asn Asp Ile Gln His Ala Gly 85 90 95 Val Gln Tyr Ile Leu Asp Ser Val Ile Ser Ala Leu Leu Ala Asp Pro 30 100 105 110 Thr Arg Arg Phe Ile Tyr Val Glu Ile Ala Phe Phe Ser Arg Trp Trp 115 120 125 His Gln Gln Thr Asn Ala Thr Gln Glu Val Val Arg Asp Leu Val Arg 130 135 140 35 Gln Gly Arg Leu Glu Phe Ala Asn Gly Gly Trp Val Met Asn Asp Glu 145 150 155 160 Ala Ala Thr His Tyr Gly Ala Ile Val Asp Gln Met Thr Leu Gly Leu 165 170 175 Arg Phe Leu Glu Asp Thr Phe Gly Asn Asp Gly Arg Pro Arg Val Ala 40 180 185 190 Trp His Ile Asp Pro Phe Gly His Ser Arg Glu Gln Ala Ser Leu Phe 195 200 205 Ala Gln Met Gly Phe Asp Gly Phe Phe Phe Gly Arg Leu Asp Tyr Gln 210 215 220 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 22 Asp Lys Trp Val Arg Met Gln Lys Leu Glu Met Glu Gln Val Trp Arg 225 230 235 240 Ala Ser Thr Ser Leu Lys Pro Pro Thr Ala Asp Leu Phe Thr Gly Val 245 250 255 Leu Pro Asn Gly Tyr Asn Pro Pro Arg Asn Leu Cys Trp Asp Val Leu 5 260 265 270 Cys Val Asp Gln Pro Leu Val Glu Asp Pro Arg Ser Pro Glu Tyr Asn 275 280 285 Ala Lys Glu Leu Val Asp Tyr Phe Leu Asn Val Ala Thr Ala Gln Gly 290 295 300 10 Arg Tyr Tyr Arg Thr Asn His Thr Val Met Thr Met Gly Ser Asp Phe 305 310 315 320 Gln Tyr Glu Asn Ala Asn Met Trp Phe Lys Asn Leu Asp Lys Leu Ile 325 330 335 Arg Leu Val Asn Ala Gln Gln Ala Lys Gly Ser Ser Val His Val Leu 15 340 345 350 Tyr Ser Thr Pro Ala Cys Tyr Leu Trp Glu Leu Asn Lys Ala Asn Leu 355 360 365 Thr Trp Ser Val Lys His Asp Asp Phe Phe Pro Tyr Ala Asp Gly Pro 370 375 380 20 His Gln Phe Trp Thr Gly Tyr Phe Ser Ser Arg Pro Ala Leu Lys Arg 385 390 395 400 Tyr Glu Arg Leu Ser Tyr Asn Phe Leu Gln Val Cys Asn Gln Leu Glu 405 410 415 Ala Leu Val Gly Leu Ala Ala Asn Val Gly Pro Tyr Gly Ser Gly Asp 25 420 425 430 Ser Ala Pro Leu Asn Glu Ala Met Ala Val Leu Gln His His Asp Ala 435 440 445 Val Ser Gly Thr Ser Arg Gln His Val Ala Asn Asp Tyr Ala Arg Gln 450 455 460 30 Leu Ala Ala Gly Trp Gly Pro Cys Glu Val Leu Leu Ser Asn Ala Leu 465 470 475 480 Ala Arg Leu Arg Gly Phe Lys Asp His Phe Thr Phe Cys Gln Gln Leu 485 490 495 Asn Ile Ser Ile Cys Pro Leu Ser Gln Thr Ala Ala Arg Phe Gln Val 35 500 505 510 Ile Val Tyr Asn Pro Leu Gly Arg Lys Val Asn Trp Met Val Arg Leu 515 520 525 Pro Val Ser Glu Gly Val Phe Val Val Lys Asp Pro Asn Gly Arg Thr 530 535 540 40 Val Pro Ser Asp Val Val Ile Phe Pro Ser Ser Asp Ser Gln Ala His 545 550 555 560 Pro Pro Glu Leu Leu Phe Ser Ala Ser Leu Pro Ala Leu Gly Phe Ser 565 570 575 Thr Tyr Ser Val Ala Gln Val Pro Arg Trp Lys Pro Gln Ala Arg Ala 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 23 580 585 590 Pro Gln Pro Ile Pro Arg Arg Ser Trp Ser Pro Ala Leu Thr Ile Glu 595 600 605 Asn Glu His Ile Arg Ala Thr Phe Asp Pro Asp Thr Gly Leu Leu Met 610 615 620 5 Glu Ile Met Asn Met Asn Gln Gln Leu Leu Leu Pro Val Arg Gln Thr 625 630 635 640 Phe Phe Trp Tyr Asn Ala Ser Ile Gly Asp Asn Glu Ser Asp Gln Ala 645 650 655 Ser Gly Ala Tyr Ile Phe Arg Pro Asn Gln Gln Lys Pro Leu Pro Val 10 660 665 670 Ser Arg Trp Ala Gln Ile His Leu Val Lys Thr Pro Leu Val Gln Glu 675 680 685 Val His Gln Asn Phe Ser Ala Trp Cys Ser Gln Val Val Arg Leu Tyr 690 695 700 15 Pro Gly Gln Arg His Leu Glu Leu Glu Trp Ser Val Gly Pro Ile Pro 705 710 715 720 Val Gly Asp Thr Trp Gly Lys Glu Val Ile Ser Arg Phe Asp Thr Pro 725 730 735 Leu Glu Thr Lys Gly Arg Phe Tyr Thr Asp Ser Asn Gly Arg Glu Ile 20 740 745 750 Leu Glu Arg Arg Arg Asp Tyr Arg Pro Thr Trp Lys Leu Asn Gln Thr 755 760 765 Glu Pro Val Ala Gly Asn Tyr Tyr Pro Val Asn Thr Arg Ile Tyr Ile 770 775 780 25 Thr Asp Gly Asn Met Gln Leu Thr Val Leu Thr Asp Arg Ser Gln Gly 785 790 795 800 Gly Ser Ser Leu Arg Asp Gly Ser Leu Glu Leu Met Val His Arg Arg 805 810 815 Leu Leu Lys Asp Asp Gly Arg Gly Val Ser Glu Pro Leu Met Glu Asn 30 820 825 830 Gly Ser Gly Ala Trp Val Arg Gly Arg His Leu Val Leu Leu Asp Thr 835 840 845 Ala Gln Ala Ala Ala Ala Gly His Arg Leu Leu Ala Glu Gln Glu Val 850 855 860 35 Leu Ala Pro Gln Val Val Leu Ala Pro Gly Gly Gly Ala Ala Tyr Asn 865 870 875 880 Leu Gly Ala Pro Pro Arg Thr Gln Phe Ser Gly Leu Arg Arg Asp Leu 885 890 895 Pro Pro Ser Val His Leu Leu Thr Leu Ala Ser Trp Gly Pro Glu Met 40 900 905 910 Val Leu Leu Arg Leu Glu His Gln Phe Ala Val Gly Glu Asp Ser Gly 915 920 925 Arg Asn Leu Ser Ala Pro Val Thr Leu Asn Leu Arg Asp Leu Phe Ser 930 935 940 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 24 Thr Phe Thr Ile Thr Arg Leu Gln Glu Thr Thr Leu Val Ala Asn Gln 945 950 955 960 Leu Arg Glu Ala Ala Ser Arg Leu Lys Trp Thr Thr Asn Thr Gly Pro 965 970 975 Thr Pro His Gln Thr Pro Tyr Gln Leu Asp Pro Ala Asn Ile Thr Leu 5 980 985 990 Glu Pro Met Glu Ile Arg Thr Phe Leu Ala Ser Val Gln Trp Lys Glu 995 1000 1005 Val Asp Gly 1010 10 <210> 22 <211> 8079 <212> DNA 15 <213> Artificial Sequence <220> <223> Expression plasmid pLamanExp1 20 <400> 22 agatcttcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat 60 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc 120 atgtccaata tgaccgccat gttggcattg attattgact agttattaat agtaatcaat 180 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa 240 25 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt 300 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta 360 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt 420 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc 480 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca 540 30 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat 600 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa 660 caactgcgat cgcccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc 720 tatataagca gagctcgttt agtgaaccgt cagatcacta gaagctttat tgcggtagtt 780 tatcacagtt aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc 840 35 tgcagtgact ctcttaaggt agccttgcag aagttggtcg tgaggcactg ggcaggtaag 900 tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag 960 aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct 1020 ctccacaggt gtccactccc agttcaatta cagctcttaa ggctagagta cttaatacga 1080 ctcactatag gctagcctcg agaattcgcc gccatgggcg cctacgcgcg ggcttcgggg 1140 40 gtctgcgctc gaggctgcct ggactcagca ggcccctgga ccatgtcccg cgccctgcgg 1200 ccaccgctcc cgcctctctg ctttttcctt ttgttgctgg cggctgccgg tgctcgggcc 1260 gggggatacg agacatgccc cacagtgcag ccgaacatgc tgaacgtgca cctgctgcct 1320 cacacacatg atgacgtggg ctggctcaaa accgtggacc agtactttta tggaatcaag 1380 aatgacatcc agcacgccgg tgtgcagtac atcctggact cggtcatctc tgccttgctg 1440 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 25 gcagatccca cccgtcgctt catttacgtg gagattgcct tcttctcccg ttggtggcac 1500 cagcagacaa atgccacaca ggaagtcgtg cgagaccttg tgcgccaggg gcgcctggag 1560 ttcgccaatg gtggctgggt gatgaacgat gaggcagcca cccactacgg tgccatcgtg 1620 gaccagatga cacttgggct gcgctttctg gaggacacat ttggcaatga tgggcgaccc 1680 cgtgtggcct ggcacattga ccccttcggc cactctcggg agcaggcctc gctgtttgcg 1740 5 cagatgggct tcgacggctt cttctttggg cgccttgatt atcaagataa gtgggtacgg 1800 atgcagaagc tggagatgga gcaggtgtgg cgggccagca ccagcctgaa gcccccgacc 1860 gcggacctct tcactggtgt gcttcccaat ggttacaacc cgccaaggaa tctgtgctgg 1920 gatgtgctgt gtgtcgatca gccgctggtg gaggaccctc gcagccccga gtacaacgcc 1980 aaggagctgg tcgattactt cctaaatgtg gccactgccc agggccggta ttaccgcacc 2040 10 aaccacactg tgatgaccat gggctcggac ttccaatatg agaatgccaa catgtggttc 2100 aagaaccttg acaagctcat ccggctggta aatgcgcagc aggcaaaagg aagcagtgtc 2160 catgttctct actccacccc cgcttgttac ctctgggagc tgaacaaggc caacctcacc 2220 tggtcagtga aacatgacga cttcttccct tacgcggatg gcccccacca gttctggacc 2280 ggttactttt ccagtcggcc ggccctcaaa cgctacgagc gcctcagcta caacttcctg 2340 15 caggtgtgca accagctgga ggcgctggtg ggcctggcgg ccaacgtggg accctatggc 2400 tccggagaca gtgcacccct caatgaggcg atggctgtgc tccagcatca cgacgccgtc 2460 agcggcacct cccgccagca cgtggccaac gactacgcgc gccagcttgc ggcaggctgg 2520 gggccttgcg aggttcttct gagcaacgcg ctggcgcggc tcagaggctt caaagatcac 2580 ttcacctttt gccaacagct aaacatcagc atctgcccgc tcagccagac ggcggcgcgc 2640 20 ttccaggtca tcgtttataa tcccctgggg cggaaggtga attggatggt acggctgccg 2700 gtcagcgaag gcgttttcgt tgtgaaggac cccaatggca ggacagtgcc cagcgatgtg 2760 gtaatatttc ccagctcaga cagccaggcg caccctccgg agctgctgtt ctcagcctca 2820 ctgcccgccc tgggcttcag cacctattca gtagcccagg tgcctcgctg gaagccccag 2880 gcccgcgcac cacagcccat ccccagaaga tcctggtccc ctgctttaac catcgaaaat 2940 25 gagcacatcc gggcaacgtt tgatcctgac acagggctgt tgatggagat tatgaacatg 3000 aatcagcaac tcctgctgcc tgttcgccag accttcttct ggtacaacgc cagtataggt 3060 gacaacgaaa gtgaccaggc ctcaggtgcc tacatcttca gacccaacca acagaaaccg 3120 ctgcctgtga gccgctgggc tcagatccac ctggtgaaga cacccttggt gcaggaggtg 3180 caccagaact tctcagcttg gtgttcccag gtggttcgcc tgtacccagg acagcggcac 3240 30 ctggagctag agtggtcggt ggggccgata cctgtgggcg acacctgggg gaaggaggtc 3300 atcagccgtt ttgacacacc gctggagaca aagggacgct tctacacaga cagcaatggc 3360 cgggagatcc tggagaggag gcgggattat cgacccacct ggaaactgaa ccagacggag 3420 cccgtggcag gaaactacta tccagtcaac acccggattt acatcacgga tggaaacatg 3480 cagctgactg tgctgactga ccgctcccag gggggcagca gcctgagaga tggctcgctg 3540 35 gagctcatgg tgcaccgaag gctgctgaag gacgatggac gcggagtatc ggagccacta 3600 atggagaacg ggtcgggggc gtgggtgcga gggcgccacc tggtgctgct ggacacagcc 3660 caggctgcag ccgccggaca ccggctcctg gcggagcagg aggtcctggc ccctcaggtg 3720 gtgctggccc cgggtggcgg cgccgcctac aatctcgggg ctcctccgcg cacgcagttc 3780 tcagggctgc gcagggacct gccgccctcg gtgcacctgc tcacgctggc cagctggggc 3840 40 cccgaaatgg tgctgctgcg cttggagcac cagtttgccg taggagagga ttccggacgt 3900 aacctgagcg cccccgttac cttgaacttg agggacctgt tctccacctt caccatcacc 3960 cgcctgcagg agaccacgct ggtggccaac cagctccgcg aggcagcctc caggctcaag 4020 tggacaacaa acacaggccc cacaccccac caaactccgt accagctgga cccggccaac 4080 atcacgctgg aacccatgga aatccgcact ttcctggcct cagttcaatg gaaggaggtg 4140 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 26 gatggttagg tctgctggga tgggccctct agagtcgacc cgggcggccg cttcccttta 4200 gtgagggtta atgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac 4260 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 4320 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt 4380 tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg 4440 5 taaaatccga taaggatcga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc 4500 ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa 4560 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4620 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4680 ctctaaatcg ggggctccct ttagggttcc gatttagagc tttacggcac ctcgaccgca 4740 10 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 4800 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 4860 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct 4920 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt 4980 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca 5040 15 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 5100 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 5160 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5220 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag 5280 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg atggttcgac cattgaactg 5340 20 catcgtcgcc gtgtcccaaa atatggggat tggcaagaac ggagacctac cctggcctcc 5400 gctcaggaac gagttcaagt acttccaaag aatgaccaca acctcttcag tggaaggtaa 5460 acagaatctg gtgattatgg gtaggaaaac ctggttctcc attcctgaga agaatcgacc 5520 tttaaaggac agaattaata tagttctcag tagagaactc aaagaaccac cacgaggagc 5580 tcattttctt gccaaaagtt tggatgatgc cttaagactt attgaacaac cggaattggc 5640 25 aagtaaagta gacatggttt ggatagtcgg aggcagttct gtttaccagg aagccatgaa 5700 tcaaccaggc caccttagac tctttgtgac aaggatcatg caggaatttg aaagtgacac 5760 gtttttccca gaaattgatt tggggaaata taaacttctc ccagaatacc caggcgtcct 5820 ctctgaggtc caggaggaaa aaggcatcaa gtataagttt gaagtctacg agaagaaaga 5880 ctaattcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa 5940 30 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga 6000 tccgcgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 6060 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 6120 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 6180 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 6240 35 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 6300 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 6360 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 6420 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 6480 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 6540 40 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 6600 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 6660 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 6720 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 6780 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 6840 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 27 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 6900 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 6960 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 7020 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 7080 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 7140 5 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 7200 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 7260 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 7320 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 7380 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 7440 10 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 7500 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 7560 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 7620 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 7680 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 7740 15 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 7800 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 7860 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 7920 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 7980 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 8040 20 tcctggcctt ttgctggcct tttgctcaca tggctcgac 8079 <210> 23 25 <211> 3761 <212> DNA <213> Homo Sapiens <400> 23 30 ggctactctc ggcttcctgg caacgccgag cgaaagctat gactgcggcc gcgggttcgg 60 cgggccgcgc cgcggtgccc ttgctgctgt gtgcgctgct ggcgcccggc ggcgcgtacg 120 tgctcgacga ctccgacggg ctgggccggg agttcgacgg catcggcgcg gtcagcggcg 180 gcggggcaac ctcccgactt ctagtaaatt acccagagcc ctatcgttct cagatattgg 240 attatctctt taagccgaat tttggtgcct ctttgcatat tttaaaagtg gaaataggtg 300 35 gtgatgggca gacaacagac ggcactgagc cctcccacat gcattatgca ctagatgaga 360 attatttccg aggatacgag tggtggttga tgaaagaagc taagaagagg aatcccaata 420 ttacactcat tgggttgcca tggtcattcc ctggatggct gggaaaaggt ttcgactggc 480 cttatgtcaa tcttcagctg actgcctatt atgtcgtgac ctggattgtg ggcgccaagc 540 gttaccatga tttggacatt gattatattg gaatttggaa tgagaggtca tataatgcca 600 40 attatattaa gatattaaga aaaatgctga attatcaagg tctccagcga gtgaaaatca 660 tagcaagtga taatctctgg gagtccatct ctgcatccat gctccttgat gccgaactct 720 tcaaggtggt tgatgttata ggggctcatt atcctggaac ccattcagca aaagatgcaa 780 agttgactgg gaagaagctt tggtcttctg aagactttag cactttaaat agtgacatgg 840 gtgcaggctg ctggggtcgc attttaaatc agaattatat caatggctat atgacttcca 900 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 28 caatcgcatg gaatttagtg gctagttact atgaacagtt gccttatggg agatgcgggt 960 tgatgacggc ccaagagcca tggagtgggc actacgtggt agaatctcct gtctgggtat 1020 cagctcatac cactcagttt actcaacctg gctggtatta cctgaagaca gttggccatt 1080 tagagaaagg aggaagctac gtagctctga ctgatggctt agggaacctc accatcatca 1140 ttgaaaccat gagtcataaa cattctaagt gcatacggcc atttcttcct tatttcaatg 1200 5 tgtcacaaca atttgccacc tttgttctta agggatcttt tagtgaaata ccagagctac 1260 aggtatggta taccaaactt ggaaaaacat ccgaaagatt tctttttaag cagctggatt 1320 ctctatggct ccttgacagc gatggcagtt tcacactgag cctgcatgaa gatgagctgt 1380 tcacactcac cactctcacc actggtcgca aaggcagcta cccgcttcct ccaaaatccc 1440 agcccttccc aagtacctat aaggatgatt tcaatgttga ttacccattt tttagtgaag 1500 10 ctccaaactt tgctgatcaa actggtgtat ttgaatattt tacaaatatt gaagaccctg 1560 gcgagcatca cttcacgcta cgccaagttc tcaaccagag acccattacg tgggctgccg 1620 atgcatccaa cacaatcagt attataggag actacaactg gaccaatctg actataaagt 1680 gtgatgttta catagagacc cctgacacag gaggtgtgtt cattgcagga agagtaaata 1740 aaggtggtat tttgattaga agtgccagag gaattttctt ctggattttt gcaaatggat 1800 15 cttacagggt tacaggtgat ttagctggat ggattatata tgctttagga cgtgttgaag 1860 ttacagcaaa aaaatggtat acactcacgt taactattaa gggtcatttc gcctctggca 1920 tgctgaatga caagtctctg tggacagaca tccctgtgaa ttttccaaag aatggctggg 1980 ctgcaattgg aactcactcc tttgaatttg cacagtttga caactttctt gtggaagcca 2040 cacgctaata cttaacaggg catcatagaa tactctggat tttcttccct tctttttggt 2100 20 tttggttcag agccaattct tgtttcattg gaacagtata tgaggctttt gagactaaaa 2160 ataatgaaga gtaaaagggg agagaaattt atttttaatt taccctgtgg aagattttat 2220 tagaattaat tccaagggga aaactggtga atctttaaca ttacctggtg tgttccctaa 2280 cattcaaact gtgcattggc cataccctta ggagtggttt gagtagtaca gacctcgaag 2340 ccttgctgct aacactgagg tagctctctt catcttattt gcaagcggtc ctgtagatgg 2400 25 cagtaacttg atcatcactg agatgtattt atgcatgctg accgtgtgtc caagtgagcc 2460 agtgtcttca tcacaagatg atgctgccat aatagaaagc tgaagaacac tagaagtagc 2520 tttttgaaaa ccacttcaac ctgttatgct ttatgctcta aaaagtattt ttttattttc 2580 ctttttaaga tgatactttt gaaatgcagg atatgatgag tgggatgatt ttaaaaacgc 2640 ctctttaata aactacctct aacactattt ctgcggtaat agatattagc agattaattg 2700 30 ggttatttgc attatttaat ttttttgatt ccaagttttg gtcttgtaac cactataact 2760 ctctgtgaac gtttttccag gtggctggaa gaaggaagaa aacctgatat agccaatgct 2820 gttgtagtcg tttcctcagc ctcatctcac tgtgctgtgg tctgtcctca catgtgcact 2880 ggtaacagac tcacacagct gatgaatgct tttctctcct tatgtgtgga aggaggggag 2940 cacttagaca tttgctaact cccagaattg gatcatctcc taagatgtac ttacttttta 3000 35 aagtccaaat atgtttatat ttaaatatac gtgagcatgt tcatcatgtt gtatgattta 3060 tactaagcat taatgtggct ctatgtagca aatcagttat tcatgtaggt aaagtaaatc 3120 tagaattatt tataagaatt actcattgaa ctaattctac tatttaggaa tttataagag 3180 tctaacatag gcttagctac agtgaagttt tgcattgctt ttgaagacaa gaaaagtgct 3240 agaataaata agattacaga gaaaattttt tgttaaaacc aagtgatttc cagctgatgt 3300 40 atctaatatt ttttaaaaca aacattatag aggtgtaatt tatttacaat aaaatgttcc 3360 tactttaaat atacaattca gtgagttttg ataaattgat atacccatgt aaccaacact 3420 ccagtcaagc ttcagaatat ttccatcacc ccagaaggtt ctcttgtata cctgctcagt 3480 cagttccttt cactcccaat tgttggcagc cattgatagg aattctatca ctataggtta 3540 gttttctttg ttccagaaca tcatgaaagc ggcgtcatgt actgtgtatt cttatgaatg 3600 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 29 gtttctttcc atcagcataa tgatttgaga ttggtccatg ttgtgtgatt cagtggtttg 3660 ttccttctta tttctgaaga gttttccatt gtatgaatat accacaattt gtttcctccc 3720 caccagtttc tgatactaca attaaaactg tctacattta c 3761 <210> 24 5 <211> 669 <212> PRT <213> Homo Sapiens <400> 24 10 Met Thr Ala Ala Ala Gly Ser Ala Gly Arg Ala Ala Val Pro Leu Leu 1 5 10 15 Leu Cys Ala Leu Leu Ala Pro Gly Gly Ala Tyr Val Leu Asp Asp Ser 20 25 30 Asp Gly Leu Gly Arg Glu Phe Asp Gly Ile Gly Ala Val Ser Gly Gly 15 35 40 45 Gly Ala Thr Ser Arg Leu Leu Val Asn Tyr Pro Glu Pro Tyr Arg Ser 50 55 60 Gln Ile Leu Asp Tyr Leu Phe Lys Pro Asn Phe Gly Ala Ser Leu His 65 70 75 80 20 Ile Leu Lys Val Glu Ile Gly Gly Asp Gly Gln Thr Thr Asp Gly Thr 85 90 95 Glu Pro Ser His Met His Tyr Ala Leu Asp Glu Asn Tyr Phe Arg Gly 100 105 110 Tyr Glu Trp Trp Leu Met Lys Glu Ala Lys Lys Arg Asn Pro Asn Ile 25 115 120 125 Thr Leu Ile Gly Leu Pro Trp Ser Phe Pro Gly Trp Leu Gly Lys Gly 130 135 140 Phe Asp Trp Pro Tyr Val Asn Leu Gln Leu Thr Ala Tyr Tyr Val Val 145 150 155 160 30 Thr Trp Ile Val Gly Ala Lys Arg Tyr His Asp Leu Asp Ile Asp Tyr 165 170 175 Ile Gly Ile Trp Asn Glu Arg Ser Tyr Asn Ala Asn Tyr Ile Lys Ile 180 185 190 Leu Arg Lys Met Leu Asn Tyr Gln Gly Leu Gln Arg Val Lys Ile Ile 35 195 200 205 Ala Ser Asp Asn Leu Trp Glu Ser Ile Ser Ala Ser Met Leu Leu Asp 210 215 220 Ala Glu Leu Phe Lys Val Val Asp Val Ile Gly Ala His Tyr Pro Gly 225 230 235 240 40 Thr His Ser Ala Lys Asp Ala Lys Leu Thr Gly Lys Lys Leu Trp Ser 245 250 255 Ser Glu Asp Phe Ser Thr Leu Asn Ser Asp Met Gly Ala Gly Cys Trp 260 265 270 Gly Arg Ile Leu Asn Gln Asn Tyr Ile Asn Gly Tyr Met Thr Ser Thr 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 30 275 280 285 Ile Ala Trp Asn Leu Val Ala Ser Tyr Tyr Glu Gln Leu Pro Tyr Gly 290 295 300 Arg Cys Gly Leu Met Thr Ala Gln Glu Pro Trp Ser Gly His Tyr Val 305 310 315 320 5 Val Glu Ser Pro Val Trp Val Ser Ala His Thr Thr Gln Phe Thr Gln 325 330 335 Pro Gly Trp Tyr Tyr Leu Lys Thr Val Gly His Leu Glu Lys Gly Gly 340 345 350 Ser Tyr Val Ala Leu Thr Asp Gly Leu Gly Asn Leu Thr Ile Ile Ile 10 355 360 365 Glu Thr Met Ser His Lys His Ser Lys Cys Ile Arg Pro Phe Leu Pro 370 375 380 Tyr Phe Asn Val Ser Gln Gln Phe Ala Thr Phe Val Leu Lys Gly Ser 385 390 395 400 15 Phe Ser Glu Ile Pro Glu Leu Gln Val Trp Tyr Thr Lys Leu Gly Lys 405 410 415 Thr Ser Glu Arg Phe Leu Phe Lys Gln Leu Asp Ser Leu Trp Leu Leu 420 425 430 Asp Ser Asp Gly Ser Phe Thr Leu Ser Leu His Glu Asp Glu Leu Phe 20 435 440 445 Thr Leu Thr Thr Leu Thr Thr Gly Arg Lys Gly Ser Tyr Pro Leu Pro 450 455 460 Pro Lys Ser Gln Pro Phe Pro Ser Thr Tyr Lys Asp Asp Phe Asn Val 465 470 475 480 25 Asp Tyr Pro Phe Phe Ser Glu Ala Pro Asn Phe Ala Asp Gln Thr Gly 485 490 495 Val Phe Glu Tyr Phe Thr Asn Ile Glu Asp Pro Gly Glu His His Phe 500 505 510 Thr Leu Arg Gln Val Leu Asn Gln Arg Pro Ile Thr Trp Ala Ala Asp 30 515 520 525 Ala Ser Asn Thr Ile Ser Ile Ile Gly Asp Tyr Asn Trp Thr Asn Leu 530 535 540 Thr Ile Lys Cys Asp Val Tyr Ile Glu Thr Pro Asp Thr Gly Gly Val 545 550 555 560 35 Phe Ile Ala Gly Arg Val Asn Lys Gly Gly Ile Leu Ile Arg Ser Ala 565 570 575 Arg Gly Ile Phe Phe Trp Ile Phe Ala Asn Gly Ser Tyr Arg Val Thr 580 585 590 Gly Asp Leu Ala Gly Trp Ile Ile Tyr Ala Leu Gly Arg Val Glu Val 40 595 600 605 Thr Ala Lys Lys Trp Tyr Thr Leu Thr Leu Thr Ile Lys Gly His Phe 610 615 620 Ala Ser Gly Met Leu Asn Asp Lys Ser Leu Trp Thr Asp Ile Pro Val 625 630 635 640 45 2013202948 08 Apr 2013 2013202948 08 Apr 2013 P 39840pc01 31 Asn Phe Pro Lys Asn Gly Trp Ala Ala Ile Gly Thr His Ser Phe Glu 645 650 655 Phe Ala Gln Phe Asp Asn Phe Leu Val Glu Ala Thr Arg 660 665 5 <210> 25 <211> 11 <212> PRT <213> Artificial Sequence 10 <220> <223> 11 residue basic peptide from HIV TAT protein <400> 25 Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 15 1 5 10 <210> 26 <211> 11 20 <212> PRT <213> Artificial Sequence <220> <223> synthetic TAT peptide 25 <400> 26 Tyr Ala Arg Ala Ala Ala Arg Gln Ala Arg Ala 1 5 10 30 2013202948 08 Apr 2013 2013202948 08 Apr 2013