AU2012329091A1 - Engineered lower eukaryotic host strains for recombinant protein expression - Google Patents

Engineered lower eukaryotic host strains for recombinant protein expression Download PDF

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Publication number
AU2012329091A1
AU2012329091A1 AU2012329091A AU2012329091A AU2012329091A1 AU 2012329091 A1 AU2012329091 A1 AU 2012329091A1 AU 2012329091 A AU2012329091 A AU 2012329091A AU 2012329091 A AU2012329091 A AU 2012329091A AU 2012329091 A1 AU2012329091 A1 AU 2012329091A1
Authority
AU
Australia
Prior art keywords
host cell
att1
seq
gene
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2012329091A
Other languages
English (en)
Inventor
Rebecca D. Argyros
Ronghua Chen
Robert C. David
Bo Jiang
Stephanie NELSON
Jun ZHUANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Sharp and Dohme LLC
Original Assignee
Merck Sharp and Dohme Ltd
Merck Sharp and Dohme LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp and Dohme Ltd, Merck Sharp and Dohme LLC filed Critical Merck Sharp and Dohme Ltd
Publication of AU2012329091A1 publication Critical patent/AU2012329091A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2012329091A 2011-10-28 2012-10-23 Engineered lower eukaryotic host strains for recombinant protein expression Abandoned AU2012329091A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161552674P 2011-10-28 2011-10-28
US61/552,674 2011-10-28
PCT/US2012/061432 WO2013062940A1 (en) 2011-10-28 2012-10-23 Engineered lower eukaryotic host strains for recombinant protein expression

Publications (1)

Publication Number Publication Date
AU2012329091A1 true AU2012329091A1 (en) 2014-05-01

Family

ID=48168381

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2012329091A Abandoned AU2012329091A1 (en) 2011-10-28 2012-10-23 Engineered lower eukaryotic host strains for recombinant protein expression

Country Status (9)

Country Link
US (1) US9328367B2 (enExample)
EP (1) EP2771461B1 (enExample)
JP (1) JP2014532406A (enExample)
KR (1) KR20140091018A (enExample)
CN (1) CN104024409A (enExample)
AU (1) AU2012329091A1 (enExample)
CA (1) CA2853341A1 (enExample)
IN (1) IN2014CN02963A (enExample)
WO (1) WO2013062940A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102574924A (zh) 2009-09-03 2012-07-11 先灵公司 抗-gitr抗体
IN2014CN02963A (enExample) 2011-10-28 2015-07-03 Merck Sharp & Dohme
US9416389B2 (en) * 2012-02-16 2016-08-16 Merck Sharp & Dohme Corp. Methods for reducing mannosyltransferase activity in lower eukaryotes
WO2014066134A1 (en) * 2012-10-22 2014-05-01 Merck Sharp & Dohme Corp. Crz1 mutant fungal cells
WO2016139279A1 (en) * 2015-03-03 2016-09-09 Sandoz Ag Constitutive yeast LLP promoter-based expression system
WO2017194699A1 (en) * 2016-05-13 2017-11-16 University Of Copenhagen A cell-based array platform
JP2018038286A (ja) * 2016-09-05 2018-03-15 国立大学法人神戸大学 新規宿主細胞及びそれを用いた目的タンパク質の製造方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060148000A1 (en) * 1999-04-21 2006-07-06 Wyeth Methods for identifying modulators of N-type ion channel inactivation
AU4363900A (en) * 1999-04-21 2000-11-02 Wyeth Methods for identifying modulators of n-type ion channel inactivation
CA2407382A1 (en) * 2000-04-24 2001-11-01 Wyeth Novel cell systems having specific interaction of peptide binding pairs
DE60139467D1 (de) 2000-05-17 2009-09-17 Mitsubishi Tanabe Pharma Corp Verfahren zur herstellung von proteinen unter reduktion von mannosephosphat in der zuckerkette und dadurch hergestelltes glykoprotein
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7029872B2 (en) 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
CA2551484C (en) 2003-12-24 2015-03-31 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
AU2005238308B8 (en) 2004-04-29 2009-10-08 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
CN101606769A (zh) 2009-07-15 2009-12-23 陈德芳 一种宝石连接结构
AR087433A1 (es) * 2011-08-08 2014-03-26 Merck Sharp & Dohme Analogos de insulina n-glicosilados
IN2014CN02963A (enExample) 2011-10-28 2015-07-03 Merck Sharp & Dohme

Also Published As

Publication number Publication date
US9328367B2 (en) 2016-05-03
CN104024409A (zh) 2014-09-03
EP2771461B1 (en) 2019-12-04
IN2014CN02963A (enExample) 2015-07-03
KR20140091018A (ko) 2014-07-18
EP2771461A4 (en) 2015-06-17
US20140302557A1 (en) 2014-10-09
WO2013062940A1 (en) 2013-05-02
JP2014532406A (ja) 2014-12-08
EP2771461A1 (en) 2014-09-03
CA2853341A1 (en) 2013-05-02

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Class et al. Patent application title: ENGINEERED LOWER EUKARYOTIC HOST STRAINS FOR RECOMBINANT PROTEIN EXPRESSION Inventors: Bo Jiang (Norwich, VT, US) Rebecca D. Argyros (Hartford, VT, US) Stephanie Nelson (White River Junction, VT, US) Robert C. Davidson (Enfield, NH, US) Robert C. Davidson (Enfield, NH, US) Ronghua Chen (Needham Heights, MA, US) Jun Zhuang (Wellesley, MA, US)

Legal Events

Date Code Title Description
MK1 Application lapsed section 142(2)(a) - no request for examination in relevant period