AU2011301856B2 - Ester pro-drugs of [3-(1-(1H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol - Google Patents
Ester pro-drugs of [3-(1-(1H-imidazol-4-yl)ethyl)-2-methylphenyl] methanolInfo
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Description
ESTER PRO-DRUGS OF [3-(1-(1H-IMIDAZOL-4-YL)ETHYL)-2-METHYLPHENYL]
METHANOL
By: Ken Chow, Liming Wang, Michael E. Garst, John E. Donello, Daniel W. Gil, and Mohammed I. Dibas
RELATED APPLICATIONS
This application claims priority to U.S. Provisional Patent Application No. 61/383,370 filed on September 16, 2010, the entire disclosure of which is incorporated herein by this reference.
BACKGROUND OF THE INVENTION
1 . Field of the invention
The invention relates to the field of pharmaceutical compounds in particular ester pro-drugs of [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, and its enantiomers. The invention further concerns processes for preparing these pharmaceutical compounds, compositions containing them and their use for the treatment and prevention of disease.
2. Summary of the related art
Three alpha-1 and three alpha-2 adrenergic receptors have been characterized by molecular and pharmacological methods. Activation of these alpha receptors evokes physiological responses with useful therapeutic applications.
Compound, 4-[1 -(2,3-dimethylphenyl)ethyl]-3H-imidazole, generically known as, medetomidine is an alpha 2 adrenergic agonist, for use in the sedation of animals. The hydrochloride salt of the (S) enantiomer of medetomidine, generically known as dexmedetomidine, (S) 4-[1 -(2,3-dimethylphenyl)ethyl]-3H-imidazole, is also indicated for use as a sedative or analgesic in cats and dogs.
The metabolite of dexmedetomidine is (S) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2- methylphenyl] methanol together with its racemic mixture, compound [3-(1 -(1 H- imidazol-4-yl)ethyl)-2-methylphenyl] methanol, are described in the literature in Journal of Chromatography, (1997), 762(1 + 2), 281 -291 by Hui, Y.-H et al.
Medetomidine Dexmedetomidine
4-(1 -(2,3-dimethylphenyl) (S)-4-(1 -(2,3-dimethylphenyl) (3-(1 -(1 H-imidazol-4-yl)ethyl) ethyl)-1 /-/-imidazole ethyl)- 1 /-/-imidazole -2-methylphenyl)methanol
CAS 86347-14-0 CAS 189255-79-6 CAS 128366-50-7
(R)-(3-(1 -(1 H-imidazol-4-yl)ethyl) (S)-(3-(1 -(1 H-imidazol-4-yl)ethyl)
-2-methylphenyl)methanol -2-methylphenyl)methanol
CAS 1240244-32-9 CAS 189255-79-6
[3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl]methanol is described in
"Synthesis of detomidine and medetomidine metabolites: 1 ,2,3-trisubstituted arenes with 4'(5')-imidazolylmethyl groups" in Journal of Heterocyclic Chemistry (1993), 30(6), (1645-1651 ) by Stoilov et al.
Kavanagh, et al. describe [3-(1 -(1 /-/-imidazol-4-yl)ethyl)-2-methylphenyl]methanol in "Synthesis of Possible Metabolites of Medetomidine {1 -(2,3-dimethylphenyl)-1 - [imidazol-4(5)-yl]ethane" in Journal of Chemical Research, Synopses (1993), (4), 152-3.
[3-(1 -(1 /-/-imidazol-4-yl)ethyl)-2-methylphenyl)methanol] is described by Salonen, et al. in "Biotransformation of Medetomidine in the Rat" in Xenobiotica (1990), 20(5), 471 -80.
PCT Int. Appl. WO 2010093930 A1 discloses [3-(1 -(1 H-imidazol-4-yl)ethyl)-2- methylphenyl]methanol and its (S) and (R) enantiomers
SUMMARY OF THE INVENTION
The present invention provides ester pro-drugs of [3-(1 -(1 H-imidazol-4-yl)ethyl)-2- methylphenyl] methanol, processes for preparing them, pharmaceutical compositions containing them and their use as pharmaceuticals. Upon hydrolytic and/or enzymatic cleavage of the ester functionality the parent compound, [3-(1 -(1 H-imidazol-4- yl)ethyl)-2-methylphenyl] methanol, is released to act as a selective modulator of the alpha 2 adrenergic receptors.
In another aspect, the present invention provides ester pro-drugs of (S) [3-(1 -(1 H- imidazol-4-yl)ethyl)-2-methylphenyl] methanol, processes for preparing them, pharmaceutical compositions containing them and their use as pharmaceuticals. Upon hydrolytic and/or enzymatic cleavage of the ester functionality the parent compound, active metabolite, (S) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, is released to act as a selective modulator of the alpha 2 adrenergic receptors.
In another aspect the present invention provides ester pro-drugs of (R) [3-(1 -(1 H- imidazol-4-yl)ethyl)-2-methylphenyl] methanol, processes for preparing them, pharmaceutical compositions containing them and their use as pharmaceuticals. Upon hydrolytic and/or enzymatic cleavage of the ester functionality the parent compound (R) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, is released to act as a selective modulator of the alpha 2 adrenergic receptors.
These novel compounds are useful for the treatment or prevention of mammals, including humans, in a range of conditions and diseases that are alleviated by alpha 2A, 2B, 2C activation, including but not limited to treating or preventing glaucoma, elevated intraocular pressure, ischemic neuropathies, optic neuropathy, pain, visceral pain, corneal pain, headache pain, migraine, cancer pain, back pain, irritable bowl syndrome pain, muscle pain and pain associated with diabetic neuropathy, the treatment of diabetic retinopathy, other retinal degenerative conditions, stroke, cognitive deficits, neropsychiatric conditions, drug dependence and addiction, withdrawal of symptoms, obsessive-compulsive disorders, obesity, insulin
resistance, stress-related conditions, diarrhea, diuresis, nasal congestion, spasticity, attention deficit disorder, psychoses, anxiety, depression, autoimmune disease, Crohn's disease, gastritis, Alzheimer's, and Parkinson's ALS other
neurodegenerative diseases, dermatological conditions, skin erythema (redness) and inflammation, acne, age related macular degeneration, wet macular
degeneration, dry macular degeneration, geographic atrophy, diabetic macular edema, tumors, wound healing, inflammation and retinal vein occlusion, enhancing vision in patients with vision loss from conditions including glaucoma, retinitis pigmentosa and neuritis secondary to multiple sclerosis, rosacea (dilation of the blood vessels just under the skin), sunburn, chronic sun damage, discreet
erythemas, psoriasis, acne rosacea, menopause-associated hot flashes, hot flashes resulting from orchiectomyatopic dermatitis, photoaging, seborrheic dermatitis, allergic dermatitis, redness of the skin, telangiectasia (dilations of previously existing small blood vessels ) of the face, rhinophymia (hypertrophy of the nose with follicular dilation), red bulbous nose, acne-like skin eruptions (may ooze or crust), burning or stinging sensation of the face, irritated and bloodshot and watery eyes, erythema of the skin, cutenous hyperactivity with dilation of blood vessels of the skin, Lyell's syndrome, Stevens-Johnson syndrome, erythema multiforme minor, erythema multiforme major and other inflammatory skin diseases.
DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to novel compounds which are ester pro-drugs of [3-(1 - (1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol of (S) [3-(1 -(1 H-imidazol-4- yl)ethyl)-2-methylphenyl] methanol and of (R) [3-(1 -(7H-imidazol-4-yl)ethyl)-2- methylphenyl] methanol as alpha-2 agonists with therapeutic utility.
In a preferred embodiment the present invention relates to ester pro-drugs of (S)-[3- (1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol as alpha-2 agonists with therapeutic utility. Upon hydrolytic or enzymatic cleavage of the ester functionality the parent compound, active metabolite, (S)-[3-(1 -(1 H-imidazol-4-yl)ethyl)-2- methylphenyl] methanol, is released to act as a selective modulator of the alpha 2 adrenergic receptors.
In one aspect of the invention, relates to a pharmaceutical composition comprising, consisting essentially of, or consisting of a therapeutically effective amount of ester pro-drugs of [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, or the enantiomers, diastereoisomers, hydrates, solvates, crystal forms and individual isomers, tautomers or a pharmaceutically acceptable salts thereof.
In another aspect of the invention, relates to a pharmaceutical composition comprising, consisting essentially of, or consisting of a therapeutically effective amount of ester pro-drugs of (S) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, or the enantiomers, diastereoisomers, hydrates, solvates, crystal forms and individual isomers, tautomers or a pharmaceutically acceptable salts thereof.
In another aspect of the invention, relates to a pharmaceutical composition comprising, consisting essentially of, or consisting of a therapeutically effective amount of ester pro-drugs of (R) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, or the enantiomers, diastereoisomers, hydrates, solvates, crystal forms and individual isomers, tautomers or a pharmaceutically acceptable salts thereof.
"Prodrugs " are frequently referred to by the term " metabolically cleavable
derivatives " which refers to compound forms which are rapidly transformed in vivo to the parent compound according to the invention, for example, by hydrolysis in blood. Thus, prodrugs are compounds bearing groups which are removed by
biotransformation prior to exhibiting their pharmacological action. Such groups include moieties which are readily cleaved in Vo from the compound bearing it, which compound after cleavage remains or becomes pharmacologically active. Such metabolically cleavable groups form a class well known to practitioners of the art. They include, but are not limited to such groups as alkanoyl (i.e. acetyl, propionyl, butyryl, and the like), unsubstituted and substituted carbocyclic aroyl (such as benzoyl, substituted benzoyl and 1 - and 2-naphthoyl), alkoxycarbonyl (such as ethoxycarbonyl), trial klysilyl (such as trimethyl- and triethylsilyl), monoesters formed with dicarboxylic acids (such as succinyl), phosphate, sulfate, sulfonate, sulfonyl, sulfinyl and the like. The compounds bearing the metabolically cleavable groups have the advantage that they may exhibit improved bioavailability as a result of enhanced solubility and/or rate of absorption conferred upon the parent compound
by virtue of the presence of the metabolically cleavable group. (T. Higuchi and V. Stella, "Pro-drugs as Novel Delivery System", Vol. 14 of the A.C.S. Symposium Series; "Bioreversible Carriers in Drug Design", ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987).
In one aspect, the invention therefore provides a compound having Formula I, its individual enantiomers, its individual diastereoisomers, its individual hydrates, its individual solvates, its individual crystal forms, its individual isomers, its individual tautomers or a pharmaceutically acceptable salt thereof,
Formula I
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl.
In a preferred aspect, the invention therefore provides a compound having Formula II, its individual enantiomers, its individual diastereoisomers, its individual hydrates, its individual solvates, its individual crystal forms, its individual isomers, its individual tautomers or a pharmaceutically acceptable salt thereof,
Formula II
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl.
In another aspect, the invention therefore provides a compound having Formula III, its individual enantiomers, its individual diastereoisomers, its individual hydrates, its individual solvates, its individual crystal forms, its individual isomers, its individual tautomers or a pharmaceutically acceptable salt thereof,
Formula III
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl.
The following paragraphs provide definitions of the various chemical moieties that make up the compounds of the invention and are intended to apply uniformly throughout the specification and claims unless expressly stated otherwise.
The term "alkyl" as used herein, is defined as including a saturated monovalent alkane moiety having straight or branched alkane moieties or combinations thereof and containing 1 -10 carbon atoms, preferably 1 -8 carbon atoms and more preferably 1 -4 carbon atoms. Alkyl moieties can optionally be substituted by, but not limited to, amino groups, aryl groups, halogens. One methylene (-CH2-) can be replaced by carbonyl, -NH-, carboxyl, amide, sulfur or by oxygen. Examples include, but are not limited to, methyl, ethyl, propyl, butyl, sec-butyl, pentyl, /'so-pentyl, neo-pentyl, hexyl, /so-hexyl, 3-methyl-butyl, 2-amino-N-isobutyl acetamide, /so-butyl, te/t-butyl, iso- propyl, ethylphenyl, methylphenyl, 2-amino-3-methyl-butanamide-N-2-methyl-1 - propyl, 1 -amino-2-methyl-prop-1 -yl.
The term "heterocycle" as used herein is defined as an aromatic or non aromatic 5 to 10 membered monocyclic or bicyclic ring containing at least one heteroatom selected from O or N or S or combinations thereof, interrupting the carbocyclic ring
structure. Heterocycles can optionally be substituted by, but not limited to, Ci-6 alkyl, amino, halogen, -O(d-6 alkyl), -OC(O)(Ci-6 alkyl), -C(O)O(Ci-6 alkyl), -NHC(O)(Ci-6 alkyl), -C(O)NH(Ci-6 alkyl), -S(Ci-6 alkyl) groups. Examples include, but are not limited to, furyl, pyrryl, pyridyl, pyrimidyl, thienyl, isothiazolyl, imidazolyl, pyrazinyl, benzofuranyl, quinolyl, isoquinolyl, benzothienyl, isobenzofuryl, pyrazolyl, indolyl, isoindolyl, benzimidazolyl, purinyl, carbazolyl, oxazolyl, thiazolyl, isothiazolyl, 1 ,2,5- thiadiazolyl, 1 ,2,4-thiadiazolyl, isooxazolyl, quinazolinyl, pyridazinyl, cinnolinyl, phthalazinyl, quinoxalinyl, xanthinyl, hypoxanthinyl, pteridinyl, 5-azacytidinyl, 5- azauracilyl, triazolopyridinyl, imidazolopyridinyl, pyrrolopyrimidinyl,
pyrazolopyrimidinyl, pyrrolidinyl, piperidinyl and piperazinyl.
The term "aryl" as used herein, is defined as including an organic moiety derived from an aromatic hydrocarbon consisting of a monocyclic or bicyclic ring containing 6-10 carbon atoms by removal of one hydrogen atom, such as phenyl or naphtyl. Aryl groups can optionally be substituted by, but not limited to, Ci-6 alkyl, amino, halogen, -O(Ci-6 alkyl), -OC(O)(Ci-6 alkyl), -C(O)O(Ci-6 alkyl), -NHC(O)(Ci-6 alkyl), - C(O)NH(Ci-6 alkyl), -S(Ci-6 alkyl) groups. Examples include, but are not limited to, phenyl, naphtyl.
The term "H" as used herein refers to a hydrogen atom. The term Ό" as used herein refers to an oxygen atom. The term "S" as used herein refers to a sulfur atom. The term "N" as used herein refers to a nitrogen atom. The term "amino" as used herein refers to a group of formula -NH2. The term "amide" as used herein refers to a group of formula -C(O)NH- or -NHC(O)-
The term "halogen", as used herein refers to an atom of chlorine, bromine, iodine or fluorine.
The term "carbonyl" as used herein refers to a group of formula -C=O.
The term "carboxyl", as used herein refers to a group of formula -C(O)O- or -OC(O)-
Generally R1 is H or C 1-3 alkyl. Preferred R1 is C 1 -3 alkyl. Most preferred R1 is methyl.
Generally R2 is H or C 1-3 alkyl. Preferred R2 is C 1 -3 alkyl. Most preferred R2 is methyl.
Generally R3 is H, C MO alkyl, heterocycle or aryl. Preferred R3 is H, phenyl or C MO alkyl. Most preferred R3 is H.
Generally R is C MO alkyl, heterocycle or aryl. Preferred R is methyl, /'so-butyl, tert- butyl, /'so-propyl, ethylphenyl, phenyl, 2-amino-1 -phenylethyl, 2-(2-amino-3-methyl- butyrylamino)-2-methyl-prop-1 -yl, 1 -amino-2-methyl-prop-1 -yl, 2-(2-amino- acetylamino)-2-methyl- prop-1 -yl. Most preferred R groups are te/t-butyl, /so-propyl.
As used herein, "tautomer" refers to the migration of protons between adjacent single and double bonds. The tautomerization process is reversible. Compounds described herein can undergo any possible tautomerization that is within the physical characteristics of the compound. The following is a tautomerization example that can occur in compounds described herein:
Compounds of the invention are:
/so-Butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2,2-Dimethyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; Acetic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
Benzoic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
3-Methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
3-Phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; 2-Amino-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2-(2-Amino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 -(1 /-/-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester;
2-(2-Annino-acetylannino)-3-nnethyl-butyric acid 3-[(S)-1 -(1 /-/-imidazol-4-yl)-ethyl]-2- methyl-benzyl ester;
2- Amino-3-phenyl-propionic acid 3-[(S)-1 -(1 /-/-imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester.
Intermediates of the invention are:
/so-Butyric acid 3-[(S)-1 -(1 -/so-butyryl-1 /-/-imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester; 2,2-Dimethyl-propionic acid 3-{(S)-1 -[1 -(2,2-dimethyl-propionyl)-1 /-/-imidazol-4-yl]- ethyl}-2-methyl-benzyl ester;
Acetic acid 3-[(S)-1 -(1 -acetyl-1 /-/-imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester;
Benzoic acid 3-[(S)-1 -(1 -benzoyl-1 /-/-imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester;
3- Methyl-butyric acid 2-methyl-3-{(S)-1 -[1 -(3-methyl-butyryl)-1 H-imidazol-4-yl]-ethyl}- benzyl ester;
Phenyl-propionic acid 2-methyl-3-{(S)-1 -[1 -(3-phenyl-propionyl)-1 /-/-imidazol-4-yl]- ethyl}-benzyl ester;
2-te/t-Butoxycarbonylamino-3-nnethyl-butyric acid 3-{(S)-1 -[1 -(2-ie/t-butoxy
carbonylamino-3-methyl-butyryl)-1 /-/-imidazol-4-yl]-ethyl}-2-methyl-benzyl ester;
2-terf-Butoxycarbonylamino-3-methyl-butyric acid 3-[(S)-1 -(1 /-/-imidazol-4-yl)-ethyl]- 2-methyl-benzyl ester;
2-(2-feri-Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-{(S)-1 - [1 -(2-teri-butoxycarbonylamino-3-methyl-butyryl)-1 H-imidazol-4-yl]-ethyl}-2-methyl- benzyl ester;
2-(2-feri-Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 - (1 /-/-imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester;
2-(2-fe/t-Butoxycarbonylannino-acetylannino)-3-nnethyl-butyric acid 3-[(S)-1 -(1 H- imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester;
2-te/t-Butoxycarbonylamino-3-phenyl-propionic acid 3-[(S)-1 -(1 /-/-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester.
Some compounds of Formula I, Formula II and Formula III and some of their intermediates have at least one stereogenic center in their structure. This
stereogenic center may be present in an (R) or (S) configuration, said (R) and (S) notation is used in correspondence with the rules described in Pure Appli. Chem. (1976), 45, 1 1 -13.
Compounds according to the present invention may exist in different polymorphic forms. Although not explicitly indicated in the above formula, such forms are intended to be included within the scope of the present invention.
Compounds of Formula I, Formula II or Formula III and their salts can be in the form of a solvate, which is included within the scope of the present invention. Such solvates include for example hydrates, alcoholates and the like.
The term "pharmaceutically acceptable salts" refers to salts or complexes that retain the desired biological activity of the above identified compounds and exhibit minimal or no undesired toxicological effects. The "pharmaceutically acceptable salts" according to the invention include therapeutically active, non-toxic base or acid salt forms, which the compounds of Formula I, Formula II or Formula III are able to form.
The acid addition salt form of a compound of Formula I, Formula II or Formula III that occurs in its free form as a base can be obtained by treating the free base with an appropriate acid such as an inorganic acid, for example but not limited to,
hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid and the like; or an organic acid such as for example but not limited to, as citric acid, acetic acid, oxalic acid, tartaric acid, succinic acid, malic acid, fumaric acid, ascorbic acid, benzoic acid, tannic acid, palmoic acid, alginic acid, polyglutamic acid, naphthalene- sulfonic acid, napthalenedisulfonic, and polygalacturonic acid as well as base addition salts such as those formed with alkali- and alkaline earth metals such as sodium, potassium and calcium and the like (Handbook of Pharmaceutical Salts, P.Heinrich Stahal& Camille G. Wermuth (Eds), Verlag Helvetica Chemica Acta- Zurich, 2002, 329-345).
The compounds can also be administered as pharmaceutically acceptable
quaternary salts known by those skilled in the art, which specifically include, but not limiting to the quaternary ammonium salt of the formula -NY+Z", wherein Y is hydrogen, alkyl, or benzyl, and Z is a counterion, including but not limited to,
chloride, bromide, iodide, -O-alkyl, toluenesulfonate, methylsulfonate, sulfonate, phosphate, or carboxylate (such as fumarate, benzoate, succinate, acetate, glycolate, maleate, malate, fumarate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate).
In another embodiment of the invention, there are provided pharmaceutical compositions including at least one compound of the invention in a pharmaceutically acceptable carrier thereof. The phrase "pharmaceutically acceptable" means the carrier, diluent or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
Pharmaceutical compositions of the present invention can be used in the form of a solid, a solution, an emulsion, a dispersion, a patch, a micelle, a liposome, and the like, wherein the resulting composition contains one or more compounds of the present invention, as an active ingredient, in admixture with an organic or inorganic carrier or excipient suitable for enteral or parenteral applications. Invention compounds may be combined, for example, with the usual non-toxic,
pharmaceutically acceptable carriers for tablets, pellets, capsules, suppositories, solutions, emulsions, suspensions, and any other form suitable for use. The carriers which can be used include but are not limited to, glucose, lactose, gum acacia, gelatin, mannitol, starch paste, magnesium trisilicate, talc, corn starch, keratin, colloidal silica, potato starch, urea, medium chain length triglycerides, dextrans, and other carriers suitable for use in manufacturing preparations, in solid, semisolid, or liquid form. In addition auxiliary, stabilizing, thickening and coloring agents and perfumes may be used. Invention compounds are included in the pharmaceutical composition in an amount sufficient to produce the desired effect upon the process or disease condition.
Pharmaceutical compositions containing invention compounds may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups or elixirs. Compositions intended for oral use may be prepared according to any method known in the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from the group
consisting of a sweetening agent such as sucrose, lactose, or saccharin, flavoring agents such as peppermint, oil of wintergreen or cherry, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets containing invention compounds in admixture with non-toxic pharmaceutically acceptable excipients may also be manufactured by known methods. The excipients used may be, for example, (1 ) inert diluents such as calcium carbonate, lactose, calcium phosphate or sodium phosphate; (2) granulating and disintegrating agents such as corn starch, potato starch or alginic acid; (3) binding agents such as gum tragacanth, corn starch, gelatin or acacia, and (4) lubricating agents such as magnesium stearate, stearic acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate may be employed. In some cases, formulations for oral use may be in the form of hard gelatin capsules wherein the invention compounds are mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin. They may also be in the form of soft gelatin capsules wherein the invention compounds are mixed with water or an oil medium, for example, peanut oil, liquid paraffin or olive oil.
The pharmaceutical compositions may be in the form of a sterile injectable suspension. This suspension may be formulated according to known methods using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally-acceptable diluent or solvent, for example, as a solution in 1 ,3- butanediol. Sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides, fatty acids (including oleic acid), naturally occurring vegetable oils like sesame oil, coconut oil, peanut oil, cottonseed oil, etc., or synthetic fatty vehicles like ethyl oleate or the like. Buffers, preservatives, antioxidants, and the like can be incorporated as required.
The present invention concerns also the use of a compound of Formula I, Formula II or Formula III, or a pharmaceutically acceptable salt thereof, for the manufacture of a
medicament for the therapeutic application. The present invention concerns also the a method for manufacturing a medicament intended for therapeutic application wherein a compound having general Formula I, Formula II or Formula III, or a pharmaceutically active derivative or salt thereof is used.
Since individual subjects may present a wide variation in severity of symptoms and each drug has its unique therapeutic characteristics, the precise mode of
administration and dosage employed for each subject is left to the discretion of the practitioner. The patient will be administered the compound orally in any acceptable form, such as a tablet, liquid, capsule, powder and the like, or other routes may be desirable or necessary, particularly if the patient suffers from nausea. Such other routes may include, without exception, transdermal, parenteral, subcutaneous, intranasal, via an implant stent, intrathecal, intravitreal, topical to the eye, back to the eye, intramuscular, intravenous, and intrarectal modes of delivery. The actual amount of the compound to be administered in any given case will be determined by a physician taking into account the relevant circumstances, such as the severity of the condition, the age and weight of the patient, the patient's general physical condition, the cause of the condition, and the route of administration. Additionally, the formulations may be designed to delay release of the active compound over a given period of time, or to carefully control the amount of drug released at a given time during the course of therapy.
Ester pro-drugs of [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, of (S) [3- (1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol or of (R) [3-(1 -(1 H-imidazol-4- yl)ethyl)-2-methylphenyl] methanol and their pharmaceutically-acceptable salts may be administered through different routes, including but not limited to topical eye drops, direct injection, application at the back of the eye or formulations that may further enhance the long duration of actions such as a slow releasing pellet, suspension, gel, or sustained delivery devices such as any suitable drug delivery system (DDS) known in the art. While topical administration is preferred, this compound may also be used in an intraocular implant as described in U.S. Patent 7,931 ,909 which is hereby incorporated by reference. Such biocompatible intraocular implants include ester pro-drugs of [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl]
methanol, of (S) [3-(1 -(1 /-/-imidazol-4-yl)ethyl)-2-methylphenyl] methanol or of (R) [3-(1 -(1 /-/-imidazol-4-yl)ethyl)-2-methylphenyl] methanol and a polymer associated with ester pro-drugs of [3-(1 -(7H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, of (S) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol or of (R) [3-(1 -(1 H- imidazol-4-yl)ethyl)-2-methylphenyl] methanol to facilitate release thereof into an eye for an extended period of time.
Ophthalmic formulations of drug products are well known in the art and described in, for example, U.S. Patent Application Publication No. 20050059583; No.
20050277584; U.S. Patent No. 7,297,679; and No. 20070015691 ; and U.S. Patent Nos. 5,474,979 and 6,582,718, the disclosures of all which are incorporated herein by reference. The ester pro-drugs of [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol, of (S) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol or of (R) [3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol may be formulated with efficacy enhancing components as disclosed in U.S. Patent Number 7,491 ,383 B2, which is hereby incorporated by reference in its entirety.
With respect to the present invention reference to a compound or compounds, is intended to encompass that compound in each of its possible isomeric forms and mixtures thereof unless the particular isomeric form is referred to specifically.
The present invention also concerns a process for preparing the compounds having general Formula I, Formula II or Formula III.
The synthetic scheme set forth below, illustrates how compounds according to the invention can be made. Those skilled in the art will be able to routinely modify and/or adapt the following scheme to synthesize any compounds of the invention covered by Formula I, Formula II or Formula III.
General scheme for synthesizing ester prodrugs of (S)-[3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol
nterme ate
(S)-(3-(1 -(1 H-imidazol-4-yl)ethyl)- 2-methylphenyl)methanol
R6 is H
In a first step (S)-[3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol (CAS 189255-79-6) can react with the desired acyl chloride, in the presence of N,N- dimethyl formamide (DMF), tertahydrofuran (THF), triethylamine (TEA) and 4- dimethyl aminopyridine (DMAP). After a typical work-up by extraction, the residue can be purified by medium pressure liquid chromatography (MPLC) (0% to 40% ethyl acetate in hexanes) to yield the intermediate compound as a solid.
In a second step, the intermediate obtained in the first reaction, can react with methanol (MeOH). The residue can be purified by MPLC (50% ethyl acetate in hexanes then 5% 7N ammonia/ methanol /dichloromethane) to yield the desired compound as a solid.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention claimed. As used herein, the use of the singular includes the plural unless specifically stated otherwise.
The present invention includes all pharmaceutically acceptable isotopically enriched compounds. Any compound of the invention may contain one or more isotopic atoms enriched or different than the natural ratio such as deuterium 2H (or D) in place of protium 1 H (or H) or use of 13 C enriched material in place of 12C and the like.
Similar substitutions can be employed for N, O and S. The use of isotopes may assist in analytical as well as therapeutic aspects of the invention. For example, use of deuterium may increase the in vivo half-life by altering the metabolism (rate) of the compounds of the invention. These compounds can be prepared in accord with the preparations described by use of isotopically enriched reagents.
The following examples are for illustrative purposes only and are not intended, nor should they be construed as limiting the invention in any manner. Those skilled in the art will appreciate that variations and modifications of the following examples can be made without exceeding the spirit or scope of the invention.
The lUPAC names of the compounds mentioned in the examples were generated with ACD version 8.
Unless specified otherwise in the examples, characterization of the compounds is performed according to the following methods:
NMR spectra are recorded on 300 MHz Varian and acquired at room temperature. Chemical shifts are given in ppm referenced either to internal TMS or to the residual solvent signal.
All the reagents, solvents, catalysts for which the synthesis is not described are purchased from chemical vendors such as Sigma Aldrich, Fluka, Lancaster, however some known reaction intermediates, for which the CAS registry number is
mentioned, were prepared in-house following known procedures.
Usually the compounds of the invention were purified by flash column
chromatography.
The following abbreviations are used in the examples:
DCM dichloromethane
MeOH methanol
CD3OD deuterated methanol
NH3 ammonia
Na2SO4 sodium sulfate
DMF N,N-dimethylformamide
MgSO4 magnesium sulfate
EtOAc ethyl acetate
/-PrOH /'so-propanol
CDCI3 deuterated chloroform
MPLC medium pressure liquid chromatography
DMF dimethylformamide
TEA triethylamine
THF tertahydrofuran
DMAP 4-dimethylaminopyridine
RT room temperature
Boc-L-Valine N-(terf-Butoxycarbonyl)-L-valine
Boc-Glycine N-(terf-Butoxycarbonyl)glycine
Boc-L-Phenylalanine N-(terf-Butoxycarbonyl)-L-phenylalanine
HCI hydrochloric acid
H2O water
EDCI 1 -ethyl-3-(3-dimethylaminopropyl) carbodiimide
NaHCOs sodium bicarbonate
Example 1
Intermediate 1
/so-Butyric acid 3-[(S)-1 -(1 -isobutyryl-1H-imidazol-4-yl)-ethyl]
-2-methyl-benzyl ester
To a solution of (S)-[3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol (1 .34g,
6.2mmol) in DMF (8ml) and THF (50ml), were added TEA (3.5ml, 24.8mmol), DMAP (780mg, 6.2mmol) and /so-butyryl chloride (2.18g, 20.5mmol). The resulting mixture was stirred at RT for 16 h, quenched with H2O and extracted with ethyl acetate. The combined organic layers were washed with brine, and dried over Na2SO4, and concentrated under reduced pressure. The residue was purified by MPLC (0% to 40% ethyl acetate in hexanes) to yield Intermediate 1 as a solid.
1 H-NMR (CD3OD, δ ppm): 1 .15 (d, J=7.03Hz, 6H), 1 .26 (d, 6H, J=6.74Hz), 1 .56 (d, J=7.03Hz, 3H), 2.34 (s, 3H), 2.58 (hept, J=7.03Hz, 1 H), 3.34(hept, J=7.74Hz, 1 H), 4.42(q, J=7.03Hz, 1 H), 5.15(s, 2H), 7.07-7.10 (m, 2H), 7.12-7.15 (m, 1 H), 7.31 (s, 1 H), 8.35 (s, 1 H).
Intermediates 2-6 were prepared in a similar manner to the method described in Example 1 starting with (S)-[3-(1 -(1 H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol. The acyl chloride used in each case and the results are tabulated below in Table 1.
Table 1
(s, 3H), 2.81 (d,
J=7.03Hz, 3H), 4.42(q, J=7.03Hz, 1 H), 5.14(s, 2H), 7.07-7.19 (m, 3H), 7.28 (s, 1 H), 8.32 (s, 1 H).
6 3-Phenyl-propionic acid 2- Phenylpropanoyl (CD3OD): 1 .52 (d,
methyl-3-{(S)-1 -[1-(3- chloride J=7.03Hz, 3H), 2.24 pheny l-propionyl)-1 H- (s, 3H), 2.64 (t, imidazol-4-yl]-ethyl}-benzyl J=7.61 Hz, 2H), 2.90
ester (t, J=7.61 Hz, 2H),
3.04 (t, J=7.61 Hz, 2H),3.24 (t,
J=7.61 Hz, 2H), 4.34 (q, J=7.03Hz, 1 H), 5.13 (s, 2H), 7.08- 7.248 (m, 14H), 8.25 (s, 1 H).
Example 2
Compound 1
/so-Butyric acid 3-[(S)-1 -(1H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester
Intermediate 1 was dissolved in MeOH (50ml) and the mixture was stirred at RT for 24 h and then concentrated under reduced pressure. The residue was purified by MPLC (50% ethyl acetate in hexanes then 5% 7N NH3/ MeOH /DCM ) to yield
Compound 1 as a solid.
1 H-NMR (CD3OD; δ ppm): 1 .15 (d, J=7.03Hz, 6H), 1 .54 (d, J=7.03Hz, 3H), 2.33 (s, 3H), 2.56 (hept, J=7.03Hz, 1 H), 4.42(q, J=7.03Hz, 1 H), 5.15(s, 2H), 6.70 (s, 1 H), 7.07-7.10 (m, 2H), 7.12-7.15 (m, 1 H), 7.55 (s, 1 H).
Compounds 2-6 and of the invention were prepared according to the procedure described in Example 2, by reacting the corresponding intermediate with methanol. The results are tabulated below in Table 2.
Example 3
Intermediate 7
2-ferf-Butoxycarbonylamino-3-methyl-butyric acid 3-{(S)-1 -[1 -(2-ferf-butoxy carbonylamino-3-methyl-butyryl)-1H-imidazol-4-yl]-ethyl}-2-methyl-benzyl ester
To a solution of (S)-[3-(1 -(1 /-/-imidazol-4-yl)ethyl)-2-nnethylphenyl] methanol (216mg, 1 .Ommol) in DMF (2ml) and THF (12ml) were added EDCI (671 mg, 3.5mmol), DMAP (427mg, 3.5mmol) and Boc-L-Valine (651 mg, 3. Ommol) . The mixture was stirred at RT for 16 h, quenched with H2O and extracted with ethyl acetate. The combined organic layers were washed with H2O, brine, and dried over Na2SO4, and
concentrated under reduced pressure. The residue was purified by a column chromatography (30% ethyl acetate in hexanes) to yield Intermediate 7 as white solid.
1 H-NMR (CD3OD; 5 ppm): 0.85-1 .01 (m, 12H), 1 .20-1 .48 (m, 18H), 1 .56 (d,
J=7.03Hz, 3H), 2.01 -2.20(m, 2H), 2.35 (s, 3H), 4.03(m, 1 H), 4.42 (q, J=7.03Hz, 1 H), 4.60-4.65 (m, 1 H), 5.15-5.29 (m, 2H), 7.10-7.20 (m, 2H), 7.20-7.25 (m, 1 H), 7.33 (s, 1 H), 8.44 (s, 1 H).
Example 4
Intermediate 8
2-ferf-Butoxycarbonylamino-3-methyl-butyric acid 3-[(S)-1 -(1H-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester
The title compound was prepared from Intermediate 7 (600mg, 0.98mmol) in 30ml of MeOH according to the procedure described in Example 2.
1 H-NMR (CD3OD; δ ppm ): 0.85-0.95 (m, 6H), 1 .42 (m, 9H), 1 .54 (d, J=7.03Hz, 3H), 2.05 (m, 1 H), 2.33 (s, 3H), 4.00 (d, J=6.15Hz, 1 H), 4.40 (q, J=7.03Hz, 1 H), 5.15- 5.28 (m, 2H), 6.67 (s, 1 H), 7.10-7.20 (m, 2H), 7.20-7.25 (m, 1 H), 7.55 (s, 1 H).
Example 5
Compound 7
2-Amino-3-methyl-butyric acid 3-[(S)-1-(1H-imidazol-4-yl)-ethyl]-
2-methyl-benzyl ester
To Intermediate 8 (390mg, 0.94mnnol) was added 4N HCI in dioxane (8ml). The resulting solution was stirred at RT for 4 hrs, then quenched with H2O, neutralized with aqueous saturated NaHCO3 and extracted with 25% isopropyl alcohol in chloroform. The combined organic layers were dried over Na2SO , and concentrated under reduced pressure. The residue was purified by a column chromatography (5% 7N NH3/MeOH in DCM) to yield Compound 7 as white solid.
1 H-NMR (CD3OD; δ ppm): 0.85 (d, J=6.74Hz, 3H), 0.91 (d, J=6.74Hz, 3H), 1 .54 (d, J=7.03Hz, 3H), 1 .96 (hept, J=6.74Hz, 1 H), 2.33 (s, 3H), 3.28 (d, J=6.74Hz, 2H), 4.42 (q, J=7.03Hz, 1 H), 5.20-5.25 (m, 2H), 6.67 (s, 1 H), 7.10-7.12 (m, 2H), 7.13- 7.20 (m, 1 H), 7.55 (s, 1 H).
Example 6
Intermediate 9
2-(2-fef -Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3- {(S)-1 -[1 -(2-ferf-butoxycarbonylamino-3-methyl-butyryl)-1 H-imidazol-4-yl]- ethyl}-2-methyl-benzyl ester
The title compound was prepared from Compound 7 (490mg, 1 .55mmol), Boc-L- Valine (1 .01 g, 4.67mmol), EDCI (1 .04g, 5.42mmol) and DMAP (671 mg, 5.5mmol) according to the procedure described in Example 3.
1 H-NMR (CD3OD; δ ppm): 0.85-0.92 (m, 12H), 1 .43 (s, 9H), 1 .55 (d, J=7.03Hz, 3H), 1 .97 (m, 1 H), 2.14 (hept, J=6.60Hz, 1 H), 2.35 (s, 3H), 3.88 (d, J=7.30Hz„ 1 H), 4.35 (d, J=6.90Hz, 1 H), 4.42(, d, J=7.03Hz, 1 H), 5.18-5.25 (m, 2H), 6.67 (s, 1 H), 7.10- 7.15 (m, 2H),7/17-7.20 (m, 1 H), 7.55 (s, 1 H).
Example 7
Intermediate 10
2-(2-fef -Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3- [(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester
The title compound was prepared from Intermediate 9 (750mg, 1 .05mmol) in 30ml of MeOH according to the procedure described in Example 2.
1 H-NMR (CD3OD; δ ppm): 0.89 (d,d , J=7.03Hz, 6H), 1 .44 (s, 9H), 1 .54 (d, J=7.33Hz, 3H), 2.14 (hept, J=6.74Hz, 1 H), 2.33 (s, 3H), 3.74 (s, 2H), 4.35-4.55 (m, 2H), 5.20 (s, 2H), 6.67 (s, 1 H), 7.10-7.17 (m, 2H), 7.19-7.23 (m, 1 H), 7.56 (s, 1 H).
Example 8
Compound 8
2-(2-Amino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 -(1H-imidazol-
4-yl)-ethyl]-2-methyl-benzyl ester
The title compound was prepared from Intermediate 10 (450mg, 0.87mmol) in 8ml of 4N HCI/Dioxane according to the procedure described in Example 5.
1 H-NMR (CD3OD; δ ppm): 0.85 (d, J=7.03Hz, 3H), 0.91 (d, J=6.74Hz, 3H), 0.92 (d, J=7.3Hz. 3H), 1 .14 (d, J=6.2Hz, 3H), 1 .54 (d, J=7.03Hz, 3H), 1 .94 (hept, J=5.2Hz, 1 H), 2.14 (hept, J=6.2Hz, 1 H), 2.33 (s, 3H), 3.18 (d, J=5.2Hz, 1 H), 4.34 (d, J=6.2Hz, 1 H), 4.42(q, J=7.03Hz, 1 H), 5.21 -5.26 (m, 2H), 6.67 (s, 1 H), 7.10-7.15 (m, 2H), 7.18-7.20 (m, 1 H), 7.55 (s, 1 H).
Example 9
Intermediate 11
2-(2-ferf-Butoxycarbonylamino-acetylamino)-3-methyl-butyric acid 3-[(S)-1-(1H- imidazol-4-yl)-ethyl]-2-methyl-benzyl ester
The title compound was prepared from Compound 8 (405mg, 1 .28mmol), Boc- Glycine(675mg, 3.86mmol), EDCI(859mg, 4.48mmol) and DMAP(547mg, 4.48mmol) according to the procedure described in Example 3. The title compound was purified by column chromatography using 5% 7N NH3/MeOH in DCM .
1 H-NMR (CD3OD; δ ppm): 0.89 (d, J=6.74Hz, 3H), 0.91 (d, J=6.74Hz, 3H), 1 .55 (d, J=7.30Hz, 3H), 2.14 (hept, J=6.74Hz, 1 H), 2.33 (s, 3H), 4.37 (d, J=5.90Hz, 1 H), 4.42(q, J=7.03Hz, 1 H), 5.20-5.25 (m, 2H), 6.67 (s, 1 H), 7.10-7.12 (m, 2H), 7.13- 7.20 (m, 1 H), 7.55 (s, 1 H).
Example 10
Compound 9
2-(2-Amino-acetylamino)-3-methyl-butyric acid 3-[(S)-1 -(1H-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester
\
H
The title compound was prepared from Intermediate 11 (320mg, 0.68mmol) with 10ml of 4N HCI/Dioxane according the procedure described in Example 5.
1 H-NMR (CD3OD; δ ppm): 0.89 (d, J=6.74Hz, 3H), 0.91 (d, J=6.74Hz, 3H), 2.14 (hept, J=6.74Hz, 1 H), 2.33 (s, 3H), 4.37 (d, J=5.90Hz, 1 H), 4.42(q, J=7.03Hz, 1 H), 5.20-5.25 (m, 2H), 6.67 (s, 1 H), 7.10-7.12 (m, 2H), 7.13-7.20 (m, 1 H), 7.55 (s, 1 H).
Example 11
Intermediate 12
2-ferf-Butoxycarbonylamino-3-phenyl-propionic acid 3-[(S)-1-(1H-imidazol-4- yl)-ethylj-2-methyl-benzyl ester
The title compound was prepared from (S)-[3-(1 -(1 H-imidazol-4-yl)ethyl)-2- methylphenyl] methanol (216mg, LOmmol), Boc-L-Phenylalanine(795mg, 3.0mmol), EDCI(671 mg, 3.5mmol) and DMAP(427mg, 3.5mmol) according to the procedure described in Example 3. Intermediate 12 was purified by a column chromatography using 35-100% ethyl acetate in hexane.
1 H-NMR (CD3OD; δ ppm): 1 .36 (s, 9H), 1 .55 (d, J=7.03Hz, 3H), 2.28 (s, 3H), 2.85- 2.95 (m, 1 H), 3.05-3.1 1 (m, 1 H), 4.38(m, 1 H), 4.40(q, J=7.03Hz, 1 H), 5.17(s, 2H), 6.69 (s, 1 H), 7.08-7.24 (m, 8H), 7.55 (s, 1 H).
Example 12
Compound 10
2-Amino-3-phenyl-propionic acid 3-[(S)-1 -(1H-imidazol-4-yl)-ethyl]-2-methyl-
The title compound was prepared from Intermediate 12 (240mg, 0.52mmol) with 8ml of 4N HCI/Dioxane according to the procedure described in Example 5.
1 H-NMR (CD3OD; δ ppm): 1 .54 (d, J=7.03Hz, 3H), 2.26 (s, 3H), 2.90-3.00 (m, 2H), 3.73 (t, J=6.40Hz, 1 H), 4.40(q, J=7.03Hz, 1 H), 5.13-5.18(m, 2H), 6.68 (s, 1 H), 7.08- 7.12 (m, 5H), 7.13-7.22 (m, 3H), 7.55 (s, 1 H).
The following assay was used to demonstrate the potency and selectivity of the compounds according to the invention.
Example 13
FLIPR Ca+2 Influx Assay
HEK 293 cells stably expressing the bovine CHA receptor, human alpha 2A receptor and the chimeric G protein Gqi5, are plated in poly-D-lysine coated 384-well plates at 20,000 - 40,000 cells per well and grown overnight in DMEM supplemented with 10% fetal bovine serum. For FLIPR (fluorometric image plate reader) evaluation, cells are washed twice with HBSS/HEPES Buffer (1 X Hanks Buffered Salt Solution, 20 mM HEPES, pH 7.4) prior to the addition of Fluo-4-AM (4 uM Fluo-4-AM, 0.04% pluronic acid in HBSS/HEPES Buffer), a calcium-sensitive dye. Cells are loaded with dye for 40 minutes at 37°C, then washed 4 times with HBSS/HEPES Buffer. For both the agonist and antagonist assay, the test compounds are tested between 0.64 nM - 10,000 nM.
For an agonist assay, the reaction is initiated by the addition of the appropriate dilutions of compounds and the transient calcium signal captured. The peak height of the calcium curve is determined and utilized for calculation of EC50 and efficacy using ActivityBase. Norepinephrine is the standard full agonist used for evaluating alpha-1 and alpha-2 receptor activity.
For an antagonist assay, the addition of the drug does not elicit a transient calcium signal. However, the antagonist blocks the transient calcium signal of the standard agonist norepinephrine in a dose-dependent manner. The residual norepinephrine peak height is compared to the non-antagonized norepinephrine peak height for the determination of % antagonism.
Table 3
In Vitro Pharmacology of (S)-[3-(1-(1H-imidazol-4-yl)ethyl)-2-methylph
methanol and its prodrugs at adrenergic receptor subtypes
EC50 (eff) nM. n/a: Not active
Claims (12)
1 . A compound of Formula I, its individual enantiomers, its individual
diastereoisomers, its hydrates, its solvates, its crystal forms, its individual isomers, its individual tautomers or a pharmaceutically acceptable salt thereof,
Formula I
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl.
2. A compound according to claim 1 of Formula II, its individual
diastereoisomers, its hydrates, its solvates, its crystal forms, its individual its individual tautomers or a pharmaceutically acceptable salt thereof,
Formula II
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl.
3. A compound according to claim 1 of Formula III, its individual enantiomers, its individual diastereoisomers, its hydrates, its solvates, its crystal forms, its individual isomers, its individual tautomers or a pharmaceutically acceptable salt thereof,
Formula III
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl.
4. A compound according to claim 2, wherein R1 is C 1-3 alkyl, R2 is C 1 -3 alkyl R3 is
H and R is C MO alkyl.
5. A compound according to claim 2, wherein R1 is methyl, R2 is methyl, R3 is H and R is Ci-4 alkyl.
6. A compound according to claim 2 selected from:
/so-Butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2,2-Dimethyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
Acetic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
Benzoic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
3-Methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; 3-Phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2-Amino-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl- benzyl ester; 2-(2-Amino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 -(1 H- imidazol-4-yl)-ethyl]-2-nnethyl-benzyl ester;
2-(2-Annino-acetylannino)-3-nnethyl-butyric acid 3-[(S)-1 -(1 /-/-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester; and
2- Amino-3-phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl- benzyl ester.
7. A compound according to claim 2 selected from:
/so-Butyric acid 3-[(S)-1 -(1 -/'so-butyryl-1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2,2-Dimethyl-propionic acid 3-{(S)-1 -[1 -(2,2-dimethyl-propionyl)-1 H-imidazol- 4-yl]-ethyl}-2-methyl-benzyl ester;
Acetic acid 3-[(S)-1 -(1 -acetyl-1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; Benzoic acid 3-[(S)-1 -(1 -benzoyl-1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
3- Methyl-butyric acid 2-methyl-3-{(S)-1 -[1 -(3-methyl-butyryl)-1 H-imidazol-4-yl]- ethyl}-benzyl ester;
Phenyl-propionic acid 2-methyl-3-{(S)-1 -[1 -(3-phenyl-propionyl)-1 H-imidazol- 4-yl]-ethyl}-benzyl ester;
2-terf-Butoxycarbonylamino-3-methyl-butyric acid 3-{(S)-1 -[1 -(2-terf-butoxy carbonylamino-3-methyl-butyryl)-1 /-/-imidazol-4-yl]-ethyl}-2-methyl-benzyl ester;
2-te/t-Butoxycarbonylamino-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester;
2-(2-fe/t-Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-{(S)-1 -[1 -(2-te/t-butoxycarbonylamino-3-methyl-butyryl)-1 H-imidazol-4-yl]-ethyl}-2- methyl-benzyl ester;
2-(2-fe/t-Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2-(2-fe/t-Butoxycarbonylamino-acetylamino)-3-methyl-butyric acid 3-[(S)-1 - (1 /-/-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; and
2-terf-Butoxycarbonylamino-3-phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4- yl)-ethyl]-2-methyl-benzyl ester.
8. A pharmaceutical composition comprising as active ingredient therapeutically effective amount of a compound of Formula I
Formula I
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl;
or a pharmaceutically acceptable salt, together with a pharmaceutically acceptable diluent or carrier.
9. A pharmaceutical composition according to claim 8, wherein the compound is of Formula II
Formula II
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl;
or a pharmaceutically acceptable salt, together with a pharmaceutically acceptable diluent or carrier.
10. A pharmaceutical composition according to claim 8 wherein the compound is of Formula III
Formula III
wherein
R1 is H or C i-3 alkyl;
R2 is H or C i-3 alkyl;
R3 is H, C M O alkyl, heterocycle or aryl; and
R is C MO alkyl, heterocycle or aryl;
or a pharmaceutically acceptable salt, together with a pharmaceutically acceptable diluent or carrier.
1 1 . A pharmaceutical composition according to claim 9 wherein the compound is selected from:
/so-Butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2,2-Dimethyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
Acetic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
Benzoic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
3-Methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; 3-Phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2-Amino-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl- benzyl ester;
2-(2-Amino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 -(1 H- imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2-(2-Amino-acetylamino)-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester; and
2-Amino-3-phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]-2-methyl- benzyl ester.
12. A pharmaceutical composition according to claim 9 wherein the compound is selected from:
/so-Butyric acid 3-[(S)-1 -(1 -isobutyryl-1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; 2,2-Dimethyl-propionic acid 3-{(S)-1 -[1 -(2,2-dimethyl-propionyl)-1 H-imidazol-4-yl]- ethyl}-2-methyl-benzyl ester;
Acetic acid 3-[(S)-1 -(1 -acetyl-1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
Benzoic acid 3-[(S)-1 -(1 -benzoyl-1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; 3-Methyl-butyric acid 2-methyl-3-{(S)-1 -[1 -(3-methyl-butyryl)-1 H-imidazol-4-yl]-ethyl}- benzyl ester;
Phenyl-propionic acid 2-methyl-3-{(S)-1 -[1 -(3-phenyl-propionyl)-1 H-imidazol-4-yl]- ethyl}-benzyl ester;
2-te/t-Butoxycarbonylamino-3-methyl-butyric acid 3-{(S)-1 -[1 -(2-terf-butoxy
carbonylamino-3-methyl-butyryl)-1 /-/-imidazol-4-yl]-ethyl}-2-methyl-benzyl ester;
2-terf-Butoxycarbonylamino-3-methyl-butyric acid 3-[(S)-1 -(1 H-imidazol-4-yl)-ethyl]- 2-methyl-benzyl ester;
2-(2-fe/t-Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-{(S)-1 - [1 -(2-te/t-butoxycarbonylamino-3-methyl-butyryl)-1 /-/-imidazol-4-yl]-ethyl}-2-methyl- benzyl ester;
2-(2-fe/t-Butoxycarbonylamino-3-methyl-butyrylamino)-3-methyl-butyric acid 3-[(S)-1 - (1 H-imidazol-4-yl)-ethyl]-2-methyl-benzyl ester;
2-(2-fe/t-Butoxycarbonylamino-acetylamino)-3-methyl-butyric acid 3-[(S)-1 -(1 H- imidazol-4-yl)-ethyl]-2-methyl-benzyl ester; and
2-te/t-Butoxycarbonylamino-3-phenyl-propionic acid 3-[(S)-1 -(1 H-imidazol-4-yl)- ethyl]-2-methyl-benzyl ester.
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Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2880578T3 (en) * | 2009-02-13 | 2021-11-24 | Allergan Inc | Pharmaceutical compositions comprising (3- (1- (1H-imidazol-4-yl) ethyl) -2-methylphenyl) methanol |
US9248123B2 (en) | 2010-01-11 | 2016-02-02 | Orexigen Therapeutics, Inc. | Methods of providing weight loss therapy in patients with major depression |
US8492557B2 (en) * | 2010-09-16 | 2013-07-23 | Allergan, Inc. | Ester pro-drugs of [3-(1-(1H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol |
EP2734200A1 (en) * | 2011-07-22 | 2014-05-28 | Allergan, Inc. | Alpha-2 adrenergic modulators for treating visual disorders mediated by central visual projections from the eye |
EP2782569A1 (en) | 2011-11-21 | 2014-10-01 | Allergan, Inc. | Pharmaceutical compositions comprising 4-[1-(2,3-dimethylphenyl)ethyl]-3h-imidazole derivatives for treating retinal diseases |
USD794650S1 (en) | 2014-11-28 | 2017-08-15 | Samsung Electronics Co., Ltd. | Display screen or portion thereof with a graphical user interface |
CN108310442B (en) * | 2017-01-17 | 2021-08-27 | 台北科技大学 | Ophthalmic composition |
KR102050506B1 (en) | 2017-06-20 | 2019-11-29 | 한국식품연구원 | Composition for preventing, improving, alleviating or treating macular disease |
CN108872431B (en) * | 2018-07-09 | 2021-03-23 | 成都倍特药业股份有限公司 | Method for detecting 4- (1- (2, 5-dimethylphenyl) ethyl) -1H-imidazole or/and hydrochloride thereof |
KR102583738B1 (en) * | 2018-08-24 | 2023-09-26 | 애들레이 노르티 바이오파마 컴퍼니 리미티드 | Highly active STING protein agonist |
MX2023012132A (en) * | 2021-04-16 | 2023-10-25 | Ads Therapeutics Llc | Alpha2 adrenergic agonist codrugs conjugated with muscarinic agonist drugs. |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG45449A1 (en) | 1992-05-13 | 1998-01-16 | Sandoz Ltd | Ophthalmic compositions |
US5459133A (en) * | 1992-06-05 | 1995-10-17 | Telor Ophthalmic Pharmaceuticals, Inc. | Methods and products for treating presbyopia |
WO1995014007A1 (en) | 1993-11-15 | 1995-05-26 | Schering Corporation | Phenyl-alkyl imidazoles as h3-receptor antagonists |
US5474979A (en) | 1994-05-17 | 1995-12-12 | Allergan, Inc. | Nonirritating emulsions for sensitive tissue |
EP0957073A1 (en) * | 1998-05-12 | 1999-11-17 | Schwarz Pharma Ag | Novel derivatives of 3,3-diphenylpropylamines |
US20040214829A1 (en) * | 2000-07-14 | 2004-10-28 | Allergan, Inc. | Compositions containing alpha-2-adrenergic agonist components |
US20020198209A1 (en) | 2001-05-03 | 2002-12-26 | Allergan Sales Inc. | Compositions having enhanced pharmacokinetic characteristics |
US7439241B2 (en) * | 2003-05-27 | 2008-10-21 | Galderma Laboratories, Inc. | Compounds, formulations, and methods for treating or preventing rosacea |
US7534805B2 (en) * | 2003-05-27 | 2009-05-19 | Societe De Conseils De Recherches Et D'applications Scientifiques (S.C.R.A.S.) | Imidazole derivatives, their preparation and their use as medicaments |
JP2007528020A (en) * | 2003-07-14 | 2007-10-04 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | Projection device |
CN102657664A (en) * | 2003-09-12 | 2012-09-12 | 阿勒根公司 | Methods and compositions for the treatment of pain and other alpha 2 adrenergic-mediated conditions |
US20050059583A1 (en) | 2003-09-15 | 2005-03-17 | Allergan, Inc. | Methods of providing therapeutic effects using cyclosporin components |
US20050244463A1 (en) * | 2004-04-30 | 2005-11-03 | Allergan, Inc. | Sustained release intraocular implants and methods for treating ocular vasculopathies |
NZ552325A (en) | 2004-05-25 | 2010-11-26 | Sansrosa Pharmaceutical Dev Inc | The use of alpha 2 andrenoceptor agonists such as brimonidine for treating inflammatory skin disorders |
US20050277584A1 (en) | 2004-06-09 | 2005-12-15 | Allergan, Inc. | Pharmaceutical compositions comprising cyclosporins |
NZ553341A (en) | 2004-09-24 | 2010-11-26 | Allergan Inc | 4-(phenylmethyl and substituted phenylmethyl)-imidazole-2-thiones acting as specific alpha2 adrenergic agonists |
US7931909B2 (en) | 2005-05-10 | 2011-04-26 | Allergan, Inc. | Ocular therapy using alpha-2 adrenergic receptor compounds having enhanced anterior clearance rates |
US20070015691A1 (en) | 2005-07-13 | 2007-01-18 | Allergan, Inc. | Cyclosporin compositions |
US7297679B2 (en) | 2005-07-13 | 2007-11-20 | Allergan, Inc. | Cyclosporin compositions |
US20070082070A1 (en) * | 2005-10-11 | 2007-04-12 | Stookey Evangeline L | Treating skin disorders |
US7902247B2 (en) | 2008-01-09 | 2011-03-08 | Allergan, Inc. | Substituted-aryl-2-phenylethyl-1H-imidazole compounds as subtype selective modulators of alpha 2B and/or alpha 2C adrenergic receptors |
CA2751651A1 (en) | 2009-02-06 | 2010-08-12 | Allergan, Inc. | Pyridine compounds as subtype selective modulators of alpha2b and/or alpha 2c adrenergic receptors |
ES2880578T3 (en) | 2009-02-13 | 2021-11-24 | Allergan Inc | Pharmaceutical compositions comprising (3- (1- (1H-imidazol-4-yl) ethyl) -2-methylphenyl) methanol |
US8394800B2 (en) * | 2009-11-19 | 2013-03-12 | Galderma Laboratories, L.P. | Method for treating psoriasis |
US8492557B2 (en) * | 2010-09-16 | 2013-07-23 | Allergan, Inc. | Ester pro-drugs of [3-(1-(1H-imidazol-4-yl)ethyl)-2-methylphenyl] methanol |
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2016
- 2016-08-18 US US15/240,219 patent/US20160354346A1/en not_active Abandoned
-
2018
- 2018-08-13 CY CY181100852T patent/CY1121024T1/en unknown
- 2018-12-27 HK HK18116626.8A patent/HK1257416A1/en unknown
-
2020
- 2020-03-20 CY CY20201100265T patent/CY1122958T1/en unknown
- 2020-04-21 CY CY20201100365T patent/CY1122809T1/en unknown
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