AU2010206035A1 - A Method for Treating Olives to be Utilised for Olive Oil Production - Google Patents

Abstract

A method for treating freshly harvested olives to substantially retain the quality of oil contained therewithin for enabling production of high quality olive oil from said treated olives at a prolonged time after harvest, the method comprising: a) washing the freshly harvested olives in a washing liquid at room temperature; b) heating the olives to a temperature sufficient to achieve enzymatic inactivation; c) rapidly cooling the heated olives to a temperature in the range of 0 to -10 OC; d) vacuum packaging a predetermined quantity of olives; and, e) storing the packed olives at a refrigerating temperature. The oil extracted therefrom after a prolonged storage time meets the standards of the IOC for Virgin Olive Oils. WASHING FRESHLY HARVESTED OLIVES IN WATER/ETHANOL HEATING TO DEACTIVATE ENZYMES 20 RAPIDLY COOLING 30 REMOVING EXCESS LIQUID FROM 40 OLIVES' SKIN (DRYING) ADDING SALTS 50 VACUUM PACKAGING 60 STORING AT REFRIGERATING TEMP. 70

Description

P/00/011 Regulation 3.2 AUSTRALIA Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT (ORIGINAL) TO BE COMPLETED BY APPLICANT Name of Applicant: Olive X-TEND Ltd. Actual Inventor(s): GERSHONY, Yariv PADAN, Nir Address for Service: EKM patent & trade marks Level 1, 38-40 Garden Street South Yarra Victoria 3141 Australia Invention Title: A Method for Treating Olives to be Utilised for Olive Oil Production The following statement is a full description of this invention, including the best method of performing it known to us: 29/07/2010, SAW103358.spc, 1 -2 A METHOD FOR TREATING OLIVES TO BE UTILISED FOR OLIVE OIL PRODUCTION FIELD OF THE DISCLOSURE 5 The present invention generally relates to olive oil production and more specifically to olive oil production at a prolonged time after olive harvesting. BACKGROUND Olive oil is produced by grinding fresh olives into a paste and extracting 10 the oil from the paste by mechanical or chemical means. The amount of oil contained in the olive depends on the particular cultivar and the degree of ripeness. Typically 1 Kg of olives yields about 150 - 200ml of oil. The oil is accumulated in the mesocarp cells, mostly in the vacuoles and to a smaller extent in the cytoplasm. Grinding or crushing of the olives breaks the olive 15 stones and flesh, creating a paste. Typically, grinding is followed by malaxation of the paste to facilitate breaking of the mesocarp cells, assisted by the sharp stone segments, thus releasing the oil contained in the vacuoles and resulting in coalescence of the oil droplets and separation of the liquids from the solids. The liquid, which contains both oil and vegetation water, is 20 extracted from the paste by applying pressure or centrifugation and is then separated into oil and water. Depending on the specific method and equipment, the separation of the paste into liquid and solid and the separation of the liquid into oil and water may be performed sequentially or concurrently. There exist various modifications of these basic steps. For example, the 25 method known as "Sinolea" is based on the different surface tension of the vegetable water and the oil. According to this method, rows of metal discs or plates are dipped into the paste. The oil adheres to the metal disks and is removed with scrapers in a continuous process while the solid and water phases stay behind. 30 The quality of olive oil depends on the extraction method, the olive cultivar, the maturity (ripeness) index of the olives and also, to a great extent, on the time delay between olive harvest and oil extraction. Because olive oil is extracted from the fruit flesh, the time elapsing from harvesting to oil extraction is very crucial. In the period between harvesting and oil extraction, 29/07/2010, SAW103358 spc, 2 -3 the fruits' enzymes are very active. The oxidation and enzymatic reactions, which begin in the fruit immediately upon harvesting, increasingly degrade the quality of the oil. Therefore, olive oil needs to be extracted from the fresh harvested olives as soon as possible, preferably within no more than 24 5 hours. Oil obtained after a longer wait is of lower quality presenting higher acidity levels. Thus, unlike seed oils (e. g., sunflower oil, sesame oil, soybeans oil, etc.) which are extracted from seeds or nuts that can be stored for prolonged time and processed all year round, the olive oil industry is a seasonal industry which depends on availability of fresh olives. Harvesting 10 time varies in each country and with the season and the cultivar. Generally, fresh olives are available for about four months during opposing seasons in the Northern and Southern hemispheres (October to January in the Northern Hemisphere and April to July in the Southern hemisphere). Another important factor that influences the oil quality is the freshness of the olive oil itself, 15 namely the storage period and the storage conditions until consumption. Olive oil, even when stored under optimal conditions, deteriorates over time. It is therefore recommended to consume olive oil close to its production time. The quality of olive oil can be characterized by various analytic and organoleptic parameters, the most common being the free fatty acid (FFA) 20 expressed as percent of oleic acid. Other characteristics include peroxide value and various positive and negative organoleptic attributes. Most countries use the International Olive Council (lOC) standards for defining oil quality and oil category. The IOC, which has a United Nations charter to develop criteria for olive oil quality and purity, consolidates standards for olive 25 oils categories as well as guidelines for analyzing and tasting the oils. For example, olive oils that can be qualified under the category of virgin olive oils are oils that are obtained from the olives by using only mechanical or other physical means which do not involve any chemical processes such as use of solvents or re-esterification. Additionally, they should meet the specific 30 standards set by the lOC with regard to the concentration of specific ingredients and organoleptic tests. Within the Virgin Olive Oil category, the oils fit for consumption are classified as Extra Virgin Olive Oil, Virgin Olive Oil and Ordinary Virgin Olive Oil according to their FFA level ( 0.8%, 2% and 5 3.3, respectively). 29/0712010, SAW103358.spc, 3 -4 Olive oil production is a traditional industry originating in the Mediterranean and known since as early as 3,000 B.C. Today, olives are cultivated in many regions of the world and olive oil is used throughout the world. However, olive oil production and consumption is still the highest in the 5 Mediterranean. The oil is considered to be a prime component of the so called 'Mediterranean diet'. In recent years, the olive oil market has been growing dramatically. The increasing interest in olive oil stems from both its unique rich taste and its health benefits and coincides with the growing public awareness to health 10 food, as well as with the general increasing interest in gourmet food. The health benefits associated with olive oil consumption include controlling LDL level while raising HDL level and reducing risk of coronary heart diseases. These health benefits are attributed to the high level of monounsaturated fatty acids, mainly oleic acid, and to the relatively high content of minor nutrients, in 15 particular anti-oxidants such as polyphenols. Studies also suggest beneficial effects of olive oil consumption on blood sugar level and on the gastrointestinal tract, including reducing the risk of ulcers, gastritis and colon cancer. Indeed, olive oil is considered by many to be superior to other vegetable oils. However, side by side with the prosperity in the olive oil 20 market, the olive oil fraud industry flourishes as well. Olive oil is one of the most adulterated agricultural products. Customers are therefore becoming increasingly aware of the possibility that oil distributed as high quality olive oil is actually adulterated oil mixed with, or even consists almost entirely of, inferior oils of lower cost. 25 The awareness to health food products, as well as to their freshness and authenticity, has brought with it a new trend of small scale production of basic food products (e.g. bread) for self consumption at home or in small food establishments such as restaurants, delicatessen and specialized boutiques. This way control over freshness and ingredients is ensured. In accordance 30 with this trend, small scale appliances, sized to be placed on a kitchen counter, such as bread machine, home-use coffee roasting device, etc., are now gaining popularity. With respect to olive oil, recent years show an increasing number of olive oil boutiques which specialize in production of high quality oil by careful selection of olives and close control over production. A 29/07/2010, SAW103358.spc, 4 -5 household countertop cold press machine for producing small quantities of olive oil designed by the inventors of the present invention is described in international publication W02010/007610. However, although olive oil can be consumed as is when freshly pressed from the fruit, the need to extract olive 5 oil within a very short time from harvesting does not enable producing olive oil out of season or in countries distant from olive growing regions. A need therefore exists for a method of providing olives suitable for oil extraction all year round and/or at locations remote from olive growing regions. In particular, a method of providing olives which retain their oil 10 qualities for a prolonged storage period without adding chemical food preservatives. A need also exists for methods for treating freshly harvested olives to substantially retain their oil quality for a prolonged time after being treated so as to enable extraction of high quality oil from the olives at a prolonged time 15 after they have been harvested. SUMMARY OF THE INVENTION According to one aspect of the present invention there is provided a method for treating freshly harvested olives to substantially retain the quality 20 of oil contained therewithin for enabling production of high quality olive oil from said treated olives at a prolonged time after harvest. The method comprises: washing the freshly harvested olives in a washing liquid at room temperature; heating the olives to a temperature sufficient to achieve enzymatic inactivation; rapidly cooling the heated olives to a temperature in the range of 25 0 to -10OC; vacuum packaging a predetermined quantity of olives; and storing the packed olives at a refrigerating temperature. The specific conditions in each step of the method are preselected in accordance with the cultivar and maturity index of the treated olives. The washing liquid may be water or ethanol or a mixture thereof. The heating may be performed by subjecting the 30 olives to microwave radiation or by immersing the olives in a liquid bath of said predetermined temperature. In accordance with some embodiments of the invention, a salt or a mineral powder is added to the treated olives before 29/07/2010, SAW103358.spc, 5 -6 packaging. The salt or mineral powder may be NaCl, KCl, NAHCO 3 , talcum powder and lime powder, or a combination thereof. According to a further aspect of the present invention there is provided a treated freshly harvested olives with retained oil qualities characterized in 5 that the oil extracted from the treated olives at a prolonged storage time after being treated is characterized by having a Free Fatty Acid (FFA) level of no more than 2%. The prolonged storage time is of at least one month. Preferable, the oil extracted from the treated olives after a storage time of at least 2 months has a Free Fatty Acid (FFA) level which does not exceed the 10 FFA level in the oil extracted at the time immediately after treatment by more than 1%. Preferably, the freshly harvested olives are treated within 24 hours from being harvested and are vacuum packaged and kept at refrigerating temperatures until use. Extraction of oil from the treated olives of the invention for the purpose of measuring FFA level or other parameters is preferably 15 performed by using an Abencor system. According to some embodiments of the invention the oil extracted from the treated olives after the prolonged storage time is further characterized by having a peroxide value that meets the standard of the International Olive Council (10C) for peroxide value in Virgin Olive Oils and/or organoleptic 20 characteristics that meet the standards of the International Olive Council (10C) for organoleptic characteristics of Virgin Olive Oils. According to yet a further aspect of the present invention there is provided a vacuum package comprising treated olives of the invention, optionally further comprising a salt or mineral powder selected from one or 25 more of the following: NaCl, KCl, NAHCO 3 , talcum powder and lime powder. BRIEF DESCRIPTION OF THE DRAWINGS The present invention will be better understood and appreciated from the following detailed description taken in conjunction with the drawings in 30 which: Fig. 1 is an overall flow chart illustrating the method of treating freshly harvested olives in accordance with an embodiment of the present invention. 29/07/2010, SAW103358.spc. 6 -7 DETAILED DESCRIPTION The present invention is generally directed at enabling off-seasonal olive oil production and/or at areas remote from olive growing regions. In accordance with the invention, freshly harvested olives are treated to 5 substantially retain their oil qualities for a prolonged time after treatment such that when stored under appropriate conditions they can be used as a "raw material" for olive oil production at a prolonged time after being harvested. The treated olives can be stored for at least one month and up to at least ten months or even for longer periods, providing the capability to produce fresh 10 olive oil all year round. Preferably, the treated olives are packaged into relatively small quantities of 0.5 to 5 Kg that can be shipped and distributed to retailers and consumers. Preferably, the treated olives maintain the appearance and texture of fresh olives. The oil extracted from the stored treated olives of the invention is 15 characterized by having Free Fatty Acidity (FFA) of no more than 2% and other characteristics, such as peroxide value and organoleptic characteristics that meet the standards of the International Olive Council (IOC) for the category of Virgin Olive Oils. In this respect, it should be emphasized that in the context of the invention, extraction of oil mainly refers to purely 20 mechanical or other physical extraction methods that do not involve chemical processing. Thus, the expression "oil extracted from the treated olives" should be understood as oil extracted by purely mechanical or other physical means. More specifically, since even when the oil is extracted by purely mechanical means, its composition may vary with specific method and specific conditions, 25 extraction of oil from the treated olives of the invention for the purpose of assessment is preferably performed using an Abencor system. The invention further provides methods for treating freshly harvested olives to substantially retain their oil qualities for a prolonged storage time under refrigerating temperatures without adding any chemical food 30 preservative. The olives selected to undergo the treatment process of the invention should be freshly harvested, high quality olives carefully selected. Any bruised, defected or otherwise poor quality olives and any twigs, stems and leaves should be carefully removed before treatment. The selected olives may 29/07/2010, SAW103358.spc, 7 -8 be of any maturity index, preferably in the range of 2-6, and of any olive cultivar, or a mixture of different cultivars. In accordance with some embodiments of the invention, the olives are organically grown olives. Fig. 1 is a flow chart of the process of treating freshly harvested olives, 5 in accordance with an embodiment of the invention. The process may be performed in a batch manner or may be a continuous process with any degree of automation. In order to obtain high quality oil from the end product of the process, the treatment is preferably performed within 24 hours from harvesting. The exact conditions in each step (e.g. temperatures, duration of 10 step, etc.) vary with the specific cultivar and maturity index of the treated olives and are pre-selected for each cultivar/maturity-index combination to optimize results. Referring to Fig. 1, the first step (10) in the process is washing the olives to remove pesticides, dust, and any dirt left on the surface of the fruit. 15 Preferably, this step is performed by immersing the olives in a liquid bath at room temperature for about 0.5 to 10 minutes. The washing liquid may be water or ethanol or a mixture thereof. Use of ethanol in the washing step provides the benefits of disinfection in addition to general cleaning. It also facilitates evaporation of the washing liquid from the olives' surface upon 20 drying. In the next step (20) the olives are heated to a predetermined temperature in the range of about from 40 to 800C for deactivating the enzymes present in the fruit so as to stop any enzymatic reactions. In accordance with one embodiment, step 20 is performed by immersing the 25 olives in a liquid bath at the selected temperature for about 2 to 10 minutes. The liquid may be water or ethanol or a mixture thereof. Alternatively, heating may be performed by subjecting the olives to microwave radiation. In the next step (30), the heated olives are rapidly cooled to a temperature in the range of 00 to -100C to prevent over-heating and possible 30 oxidation or any other reactions. Step 30 may be performed by immersing the olives in an ice-water, by cold air blowers or by any other method known in the art. 29107/2010, SAW103358.spc, 8 -9 In the next step (40) the olives are dried, if necessary, by blowing dry air to remove any water left on their skins and in the following step (60) predetermined quantities of olives are packaged in sealed vacuum packages in air impervious flexible film, using standard equipment typical in the art. The 5 packaged olives are then stored at regular refrigeration temperatures of 0 40C (step 70). Once the treated olives are sealed in packages, the packages can be handled in an integral manner from the packaging area to storage and through distribution channels to the final consumption site using regular refrigeration equipment. 10 Optionally, different salts and/or mineral powders may be added to the olives (step 50) before packaging for inhibiting mold growth and for maintaining the fruit texture by lowering the water activity of the olives. The added salts and mineral powders also facilitate phase separation between the oil and vegetable water during the oil extraction process. Optional added 15 minerals are selected from sodium chloride, potassium chloride, sodium bicarbonate, micronized talc and lime powder. The amount of added salts and/or minerals is preferably 50g or more per 1 Kg of packed olives. Example 1: Fresh olives from three different harvests in winter 2007-2008 were 20 treated according to a first treatment procedure (treatment procedure 1) of the invention as in the following: After cleaning and washing, the freshly harvested olives were subjected to microwave radiation of 600 Watt for 1 minute, followed by immediate cooling in ice water. The olives were dried, vacuum packed into packages of 700g (using a LEVAC C30 M8 vacuum packaging 25 machine, manufactured by Lerica, Italy) and stored at 40C. At one, three and five months after treatment, oil was extracted from one or more packages of the olives using an Abencor system (MC2 Ingenieria y Sistemas, Barcelona, Spain). Organoleptic characteristics assessment and analysis of the extracted oil for Free acidity (FFA), peroxide level and polyphenols concentration were 30 carried out according to the IC guidelines. The results are summarized in Table 1. Analysis methods: Determination of free acidity and peroxide value were carried out following the analytical methods recommended by EC 29/0712010, SAW103358.spc, 9 -10 (2568/91) (19). Free acidity given as a percentage of oleic acid, was determined by titration of a solution of oil in ethanol-ether (1:1) with ethanolic potash. Peroxide value, expressed in milliequivalents of active oxygen per kilogram of oil (meq/kg) was determined as follows: a mixture of oil and 5 isooctane-acetic acid 3:2 was left to react in darkness with a potassium iodide solution; the free iodine was then titrated with a sodium thiosulfate solution. Extraction and Analysis of Phenolics.: Phenolic fraction was isolated from a solution of oil in hexane by triple extraction with water-methanol (60:40 v/v). Total phenols, expressed as catechin equivalents (ppm), were determined 10 with a UV-visible spectrophotometer (Beckman Coulter, Inc. Fullerton, California, US) at 735nm using the Folin-Ciocalteu reagent (20). Ortho diphenols fraction for HPLC analysis was extracted according to Mateos et al. 2001 (21). Phenolic compounds were separated by a HPLC system consisting of a TSP P4000 pump coupled to a UV 6000LP Diode Array detector and with 15 Chromquest software. Analytical separation was achieved on an Ultrasphere RP-C18, 5 pm column (250x4.6 mm i.d.) (Beckman, USA). Phenolic compounds were monitored at 280 nm, and identified on the basis of their absorbance spectrum and retention times compared to those of standard compounds following the IOC official method (COI/T.20/Doc No. 29) (22). 20 29/07/2010, SAW103358 spc, 10 - 11 Tablel: Assessment of oil extracted from three harvests (treatment procedure # 1): Variety, Storage FFA 1 Peroxide Polyphenols Organoleptic orchard & period (%) Value2 content in assessment Date (months) [mg/kg Oil] Bitterness level 1, 1 0.23 3.28 210.0 Picual, pungency level 0 Kish 3 0.30 2.03 141.0 14.12.2007 5 0.64 8.76 142.0 Fruity and grassy oil 0 0.10 2.20 175.0 (level 4-5), Bitterness level 0, pungency level Picual, 3 Revivim, 27.12.2007 1 0.64 3.17 125 3 1.57 3.55 108.0 5 1.45 6.80 130.0 Strong "apple smell" and aroma, Bitterness 0 0.06 5.50 168.0 level 1, pungency level 2-3 Picholine, Gshur Fine "apple smell", 03.01.2008 1 0.24 2.48 136.0 Bitterness level 1, pungency level 2 3 0.27 3.35 100.0 5 0.35 4.08 111.0 29/07/2010, SAW103358.spc, 11 - 12 FFA is expressed as % free oleic acid (grams of oleic acid per 100 grams oil); 2 Peroxide level measured in millieq. of oxygen / kg Oil The results clearly demonstrate that the free acid level remains below 5 2% for all samples, including those extracted after 5-months storage period. The peroxide value remains well below the standard of the IOC for Virgin Olive Oil ( 20 millieq. Peroxide oxygen per Kg/oil) Example 2: Picual olives from the same harvest (Revivim, 18.12.2008) were 10 treated by four different procedures as in the following: Treatment procedure # 2: 1min in 70% EtOH / Ice water cooling / Vacuum packaging / 4 C* storage Treatment procedure # 3: 1min in 70% EtOH / Ice water cooling / 100 gr KCI per 700 gr olives/ Vacuum packaging / 4 C* storage 15 Treatment procedure # 4: 4min at 600 / Ice water cooling / Vacuum packaging / 4 C* storage Treatment procedure # 5: 4min at 600 / Ice water cooling / 100 gr KCI per 700 gr olives / Vacuum packaging / 4 C* storage. Oil was extracted from treated olives immediately after treatment (t=0) 20 and after 2.5 month storage period. Oil assessment and analysis was performed as in Example 1. The results are summarized in Table 2. 29107/2010, SAW103358.spc, 12 -13 Table 2: Assessment of oil extracted from olives of the same harvest treated according to different procedures: Treatment Storage FFA 1 (%) Peroxide Polyphenols Organoleptic procedure period Value 2 content in assessment number (months) [mg/kg Oil] Strong fruitness, "orange smell", o 0.17 6.34 103.84 Bitterness level 2 2 3, pungency level 0 2.5 0.37 5.89 65.09 0 0.13 5.89 281.30 3 2.5 0.20 5.43 189.86 Delicate oil, fruity 0 0.24 11.10 87.18 4 aroma 2.5 0.35 4.98 33.71 Good oil, neutral Good oil, balanced (slightly 0 0.21 7.93 91.05 strong), low 5 bitterness, pungency level 2 2.5 0.22 8.15 54.24 Good oil Example 3: 5 The procedure 5 in Example 2 was repeated with Koroneiki olives. Oil was extracted from treated olives immediately after treatment (t=0) and after 2.5 month storage period. Oil assessment and analysis was performed as in Example 1. The results are summarized in Table 3. 29/07/2010, SAW103358.spc, 13 -14 Table 3: Assessment of oil extracted from Koroneiki olives treated according to treatment procedure # 5: Treatment Storage FFA 1 (%) Peroxide Polyphenols Organoleptic number period Value 2 content in assessment (months) [mg/kg Oil] Good oil, bitterness level 5, 0 0.24 4.53 413.42 pungency level 2, fruity 5 and grassy Fruity-grassy oil 2.5 0.35 5.89 370.80 (balanced), high bitterness As can be clearly seen, the oils extracted in both Examples 2 and 3 5 meet the criteria of the 10C for Extra Virgin Olive Oil with respect to free acidity and peroxide level (50.8%, 520 millieq. Peroxide oxygen per Kg oil, respectively). It will be appreciated by persons skilled in the art that the present invention is not limited to what has been particularly shown and described 10 hereinabove. Rather the scope of the present invention is defined only by the claims which follow. Where the terms "comprise", "comprises", "comprised" or "comprising" are used in this specification, they are to be interpreted as specifying the presence of the stated features, integers, steps or components referred to, but 15 not to preclude the presence or addition of one or more other features, integers, steps, components to be grouped therewith. 29/07/2010, SAW103358 spc, 14

Claims (21)

1. A method for treating freshly harvested olives to substantially retain the quality of oil contained therewithin for enabling production of high quality olive 5 oil from said treated olives at a prolonged time after harvest, the method comprising: washing the freshly harvested olives in a washing liquid at room temperature; heating the olives to a temperature sufficient to achieve enzymatic 10 inactivation; rapidly cooling the heated olives to a temperature in the range of 0 to 10 C; vacuum packaging a predetermined quantity of olives; and, storing the packed olives at a refrigerating temperature. 15

2. The method of claim 1, wherein said washing liquid is water or ethanol or a mixture thereof.

3. The method of claim 1 or claim 2, wherein said heating is to a 20 predetermined temperature in the range of 40 to 800C.

4. The method of any one of the preceding claims, wherein said heating is performed by subjecting the olives to microwave radiation. 25

5. The method of any one of claims 1 to 3, wherein said heating is performed by immersing the olives in a liquid bath of said predetermined temperature.

6. The method of any one of the preceding claims, further comprising a 30 step of adding a salt or a mineral powder to the treated olives.

7. The method of claim 6, wherein said salt or mineral powder is selected from the group consisting of NaCl, KCl, NAHCO 3 , talcum powder and lime powder, or a combination thereof. 29/07/2010, SAW103358.spc, 15 - 16

8. The method of any one of the preceding claims, wherein the specific conditions in each step of the method are preselected in accordance with the cultivar and maturity index of the treated olives. 5

9. Treated freshly harvested olives with retained oil qualities characterized in that the oil extracted from said treated olives at any time of up to at least one month after being treated is characterized by having a Free Fatty Acid (FFA) level of no more than 2%. 10

10. The treated olives of claim 9, wherein said Free Fatty Acid (FFA) level is 50.8 %.

11. The treated olives of claim 9 or claim 10, wherein said oil is further 15 characterized by having a peroxide value that meets the standard of the International Olive Council (lOC) for peroxide value of Virgin Olive Oils.

12. The treated olives of any one of claims 9 to 11, wherein said oil is further characterized by having organoleptic characteristics that meet the 20 standard of the International Olive Council (lOC) for organoleptic attributes of Virgin Olive Oil.

13. The treated olives of any one of claims 9 to 12, wherein the oil is extracted using an Abencor system. 25

14. The treated freshly harvested olives of any one of claims 9 to 12, wherein the oil extracted from said treated olives at any time of up to at least two months after being treated has a Free Fatty Acid (FFA) level which does not deviate from the FFA level in the oil extracted at the time immediately after 30 treatment by more than 1 %.

15. The treated olives of any one of claims 9 to 14, wherein the freshly harvested olives are treated within 24 hours from being harvested. 29/07/2010. SAW103358.spc. 16 -17

16. The treated olives of claim 9, wherein the oil extracted from said treated olives by an Abencor system at any time of up to at least three months after being treated is characterized by having a Free Fatty Acid (FFA) level of no more than 2%. 5

17. The treated olives of claim 9, wherein the oil extracted from said treated olives by an Abencor system at any time of up to at least six months after being treated is characterized by having a Free Fatty Acid (FFA) level of no more than 2%. 10

18. The treated olives of any one of claims 9 to 17, wherein said olives are vacuum packaged and are kept at refrigerating temperatures until use.

19. A vacuum package containing treated olives according to any one of 15 claims 9 to 18.

20. The vacuum package of claim 19, further comprising a salt or a mineral powder selected from the group consisting of NaCl, KCI, NAHCO 3 , talcum powder and lime powder, or a combination thereof. 20

21. A method for treating freshly harvested olives, substantially as hereinbefore described with reference to the accompanying drawings. DATED this 29t' day of July 2010 25 Olive X-TEND Ltd. By Their Patent Attorneys EKM patent & trade marks 29/07/2010. SAW103358.spc, 17