AU2008247457A1 - [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] -5-chloro-thiophen-2-yl-sulfonylurea salts, in different crystalline forms, pharmaceutical compositions thereof - Google Patents

Description

WO 2008/137809 PCT/US2008/062584 [4-(6-FLUORO-7-METHYLAMINO-2,4-DIOXO-1,4-DIHYDRO-2H QUINAZOLIN-3-YL)-PHENYL]-5-CHLORO-THIOPHEN-2-YL SULFONYLUREA SALTS, FORMS AND METHODS RELATED THERETO 5 CROSS-REFERENCE TO RELATED APPLICATIONS [0001] The present application claims priority to U.S. Provisional Application No. 60/927,328, filed May 2, 2007, which is herein incorporated by reference in its entirety for all purposes. 10 BACKGROUND OF THE INVENTION [00021 Thrombotic complications are a major cause of death in the industrialized world. Examples of these complications include acute myocardial infarction, unstable angina, chronic stable angina, transient ischemic attacks, strokes, peripheral vascular disease, preeclampsia/eclampsia, deep venous thrombosis, embolism, disseminated intravascular 15 coagulation and thrombotic cytopenic purpura. Thrombotic and restenotic complications also occur following invasive procedures, e.g., angioplasty, carotid endarterectomy, post CABG (coronary artery bypass graft) surgery, vascular graft surgery, stent placements and insertion of endovascular devices and prostheses, and hypercoagulable states related to genetic predisposition or cancers. It is generally thought that platelet aggregates play a critical role in 20 these events. Blood platelets, which normally circulate freely in the vasculature, become activated and aggregate to form a thrombus from disturbed blood flow caused by ruptured atherosclerotic lesions or by invasive treatments such as angioplasty, resulting in vascular occlusion. Platelet activation can be initiated by a variety of agents, e.g., exposed subendothelial matrix molecules such as collagen, or by thrombin which is formed in the 25 coagulation cascade. 100031 An important mediator of platelet activation and aggregation is ADP (adenosine 5' diphosphate) which is released from blood platelets in the vasculature upon activation by various agents, such as collagen and thrombin, and from damaged blood cells, endothelium or tissues. Activation by ADP results in the recruitment of more platelets and stabilization of 30 existing platelet aggregates. Platelet ADP receptors mediating aggregation are activated by ADP and some of its derivatives and antagonized by ATP (adenosine 5'-triphosphate) and 1 WO 2008/137809 PCT/US2008/062584 some of its derivatives (Mills, D. C. B. (1996) Thromb. Hemost. 76:835-856). Therefore, platelet ADP receptors are members of the family of P2 receptors activated by purine and/or pyrimidine nucleotides (King, B. F., Townsend-Nicholson, A. & Burnstock, G. (1998) Trends Pharmacol. Sci. 19:506-514). 5 [0004] Recent pharmacological data using selective antagonists suggests that ADP dependent platelet aggregation requires activation of at least two ADP receptors (Kunapuli, S. P. (1998), Trends Pharmacol Sci. 19:391-394; Kunapuli, S. P. & Daniel, J. L. (1998) Biochem. J. 336:513-523; Jantzen, H. M. et al. (1999) Thromb. Hemost. 81:111-117). One receptor appears to be identical to the cloned P2Yi receptor, mediates phospholipase C 10 activation and intracellular calcium mobilization and is required for platelet shape change. The second platelet ADP receptor important for aggregation mediates inhibition of adenylyl cyclase. Based on its pharmacological and signaling properties this receptor has been provisionally termed P 2 YADP (Fredholm, B. B. et al. (1997) TIPS 18:79-82), P2TAC (Kunapuli, S. P. (1998), Trends Pharmacol. Sci. 19:391-394) or P2Ycyc (Hechier, B. et al. 15 (1998) Blood 92, 152-159). More recently, molecular cloning of this receptor (Hollopeter, G. et al. (2001) Nature 409: 202-207) has revealed that it is a new member of the G-protein coupled family and is the target of the thienopyridine drugs ticlopidine and clopidogrel. The nomenclature given to this receptor is P2Y 12 . [0005] Various directly or indirectly acting synthetic inhibitors of ADP-dependent platelet 20 aggregation with antithrombotic activity have been reported. The orally active antithrombotic thienopyridines ticlopidine and clopidogrel inhibit ADP-induced platelet aggregation, binding of radiolabeled ADP receptor agonist 2-methylthioadenosine 5' diphosphate to platelets, and other ADP-dependent events indirectly, probably via formation of an unstable and irreversible acting metabolite (Quinn, M. J. & Fitzgerald, D. J. (1999) 25 Circulation 100:1667-1667). Some purine derivatives of the endogenous antagonist ATP, e.g., AR-C (formerly FPL or ARL) 67085MX and AR-C6993 1Mx, are selective platelet ADP receptor antagonists which inhibit ADP-dependent platelet aggregation and are effective in animal thrombosis models (Humphries et al. (1995), Trends Pharmacol. Sci. 16, 179; Ingall, A. H. et al. (1999) J Med. Chem. 42, 213-230). Novel triazolo [4,5-d] pyrimidine 30 compounds have been disclosed as P2T -antagonists (WO 99/05144). Tricyclic compounds as platelet ADP receptor inhibitors have also been disclosed in WO 99/36425. The target of these antithrombotic compounds appears to be P 2

YI

2 , the platelet ADP receptor mediating inhibition of adenylyl cyclase. 2 WO 2008/137809 PCT/US2008/062584 [0006] Despite these compounds, there exists a need for more effective platelet ADP receptor inhibitors. In particular, there is a need for platelet ADP receptor inhibitors having antithrombotic activity that are useful in the prevention and/or treatment of cardiovascular diseases, particularly those related to thrombosis. 5 10007] In addition, while biological activity is a sine non qua for an effective drug, the compound must be capable of large scale manufacturing and the physical properties of the compound can markedly impact the effectiveness and cost of a formulated active ingredient. Salts of acidic and basic compounds can alter or improve the physical properties of a parent compound. These salt forming agents, however, must be identified empirically by the 10 pharmaceutical chemist since there is no reliable method to predict the influence of a salt species on the behavior of a parent compound in dosage forms. Effective screening techniques, which potentially could simplify the selection process, are unfortunately absent (G. W. Radebaugh and L. J. Ravin Preformulation. In, Remington: The Science and Practice of Pharmacy; A. R. Gennaro Ed.; Mack Publishing Co. Easton, Pa., 1995; pp 1456-1457). 15 [00081 Amorphous and different crystalline forms (polymorphic or solvated) of salts are frequently encountered among pharmaceutically useful compounds. Polymorphism is the ability of any element or compound to crystallize in more than one lattice arrangement. Physical properties including solubility, melting point (endotherm onset in DSC analysis), density, hardness, crystal shape and stability can be different for different solid forms of the 20 same chemical compound. [00091 Crystalline and amorphous forms may be characterized by scattering techniques, e.g., X-ray powder diffraction, by spectroscopic methods, e.g., infra-red, solid state "C and 1 9 F nuclear magnetic resonance spectroscopy and by thermal techniques, e.g, differential scanning calorimetry (DSC) or thermogravimetric analysis (TGA). Although the intensities 25 of peaks in the X-ray powder diffraction patterns of different batches of a polymorph may vary slightly, the peak locations are characteristic for a specific crystalline solid form. Additionally, infrared, Raman and thermal methods have been used to interpret differences between crystalline forms. Crystalline and amorphous forms may be characterized by data from the X-ray powder diffraction pattern determined in accordance with procedures which 30 are known in the art (see J. Haleblian, J Pharm. Sci. 1975 64:1269-1288, and J. Haleblain and W. McCrone, J Pharm. Sci. 1969 58:911-929). 3 WO 2008/137809 PCT/US2008/062584 [00101 As discussed in U.S. Patent Application No. 11/556,490, the free acid compound of the salt of formula I (Formula I) is a potent platelet ADP receptor inhibitor. Surprisingly and unexpectedly, it was found that certain salts and crystalline forms of the present invention show improved properties including but not limited to crystallinity, thermal, hydrolytic and 5 hygroscopic stability and purity. In addition, the salts of Formula I of the present invention are useful for the treatment of undesired thrombosis in mammals. SUMMARY OF THE INVENTION 10011] In one aspect, the present invention provides a salt comprising a compound Formula I: F H N N S HC HN \/0 10 0 and an ion selected from the group consisting of calcium, L-lysine, ammonium, magnesium, L-arginine, tromethamine, N-ethylglucamine and N-methylglucamine. In another aspect, the invention provides crystalline solid forms of the sodium, potassium, 15 calcium, L-lysine, ammonium, tromethamine salts of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo 1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea. [00121 In another aspect, the invention provides pharmaceutical compositions for preventing or treating thrombosis and thrombosis related conditions in a mammal. The compositions contain a therapeutically effective amount of one or more salts of formula (I) or 20 a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier or excipient. The invention further provides a method for preventing or treating thrombosis and thrombosis related conditions in a mammal by administering a therapeutically effective amount of a salt of formula (I). [0013] In still another aspect, the present invention provides methods for preparing salts of 25 formula (I), their crystalline solid and amorphous forms and pharmaceutical compositions for preventing or treating thrombosis and thrombosis related conditions in a mammal. [0014] In some embodiments, the present invention provides a method for preventing or treating a condition in a mammal characterized by undesired thrombosis comprising 4 WO 2008/137809 PCT/US2008/062584 administering to the mammal a therapeutically effective amount of a salt of Formula I or the salt of Formula I having a crystalline polymorph form including the sodium and potassium salts. In another embodiment, the condition is selected from the group consisting of acute coronary syndrome, myocardial infarction, unstable angina, refractory angina, occlusive 5 coronary thrombus occurring post-thrombolytic therapy or post-coronary angioplasty, a thrombotically mediated cerebrovascular syndrome, embolic stroke, thrombotic stroke, transient ischemic attacks, venous thrombosis, deep venous thrombosis, pulmonary embolus, coagulopathy, disseminated intravascular coagulation, thrombotic thrombocytopenic purpura, thromboanglitis obliterans, thrombotic disease associated with heparin-induced 10 thrombocytopenia, thrombotic complications associated with extracorporeal circulation, thrombotic complications associated with instrumentation, and thrombotic complications associated with the fitting of prosthetic devices. [0015] In another embodiment, the present invention provides a method for inhibiting the coagulation of a blood sample comprising the step of contacting the sample with a salt 15 comprising the salt of formula I including in a crystalline solid form. [00161 In a further embodiment, the present invention provides a method of preparing a salt of formula I comprising contacting a base with a compound of formula II: F 0 0 C HN_ Hs H3C N N \/ NH S"

HN-

0 II 20 or a salt thereof under conditions to form the salt of Formula I. [0017] In some embodiments, the conditions are nucleophilic addition conditions and comprise use of a non-polar, aprotic solvent. In some other embodiments, the solvent is a member selected from the group consisting of tetrahydrofuran, diethyl ether, dimethoxymethane, dioxane, hexane, methyl tert-butyl ether, heptane, and cyclohexane. In 25 some embodiments, the salt of the compound of Formula II is an acid salt. [00181 In some embodiments, the present invention provides a method of preparing a salt of formula I wherein the method is performed at a temperature of less than 10 'C. 5 WO 2008/137809 PCT/US2008/062584 [0019] In a further embodiment, the present invention provides a method of preparing a salt of formula I wherein the compound having Formula I is afforded in a yield of at least 50%. In another embodiment, the compound having Formula I is afforded in a yield of at least 65%. In still another embodiment, the compound having Formula I is afforded in a yield of 5 at least 75%. [0020] In another embodiment, the present invention provides a method of making the salt of formula I on a gram scale or a kilogram scale. BRIEF DESCRIPTION OF THE DRAWINGS 10 [00211 Figure 1 provides structure of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium and/or sodium salt. [00221 Figure 2a shows an X-ray powder diffraction (XRPD) of crystalline solid form A of [4-(6-fluoro-7-methylamino-2,4-dioxo-1, 4 -dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro 15 thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate. Figure 2b shows an XRPD of crystalline solid form A of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate showing peak position information. [00231 Figure 3a shows an XRPD of crystalline solid form B of [4-(6-fluoro-7 20 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt hemi hydrate. Figure 3b shows an XRPD of crystalline solid form B of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5 chloro-thiophen-2-yl-sulfonylurea potassium salt hemi hydrate showing peak position information. 25 [0024] Figure 4 shows an XRPD of the amorphous [ 4 -(6-fluoro-7-methylamino-2,4-dioxo 1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt. [0025] Figure 5 shows a Fourier-transformed infrared spectra (FT-IR) of crystalline solid form A of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] 5-chloro-thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate. 6 WO 2008/137809 PCT/US2008/062584 [0026] Figure 6 shows a Fourier-transformed infrared spectra (FT-IR) of crystalline solid form B of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5 chloro-thiophen-2-yl-sulfonylurea potassium salt hemi hydrate. [0027] Figure 7 shows the FT-IR of an amorphous form of [ 4

-(

6 -fluoro-7-methylamino 5 2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt. [0028] Figure 8 shows the 'H-NMR of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate. [00291 Figure 9 shows the 'H-NMR of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 10 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt hemi hydrate. [00301 Figure 10 shows the 'H-NMR of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt. [00311 Figure 11 provides the gravimetric vapour sorption (GVS) data of crystalline solid 15 form A of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] 5-chloro-thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate (form A). [00321 Figure 12a provides the gravimetric vapour sorption (GVS) data of crystalline solid form B of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5 chloro-thiophen-2-yl-sulfonylurea potassium salt hemi hydrate. The sample was recovered 20 after the completion of the GVS experiment and re-examined by XRPD (form B). The results (Figure 12b) show that no phase change has occurred over the course of the GVS experiment. The change in intensity of the peak at ca. 5.4' 20, is a preferred orientation effect. [0033] Figure 13 provides the gravimetric vapour sorption (GVS) data of amorphous form 25 of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea sodium salt. [0034] Figure 14 provides the differential scanning calorimetry (DSC) data of crystalline solid form A of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl) phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate. 7 WO 2008/137809 PCT/US2008/062584 [00351 Figure 15 provides the TGA data of crystalline solid form A of [4-(6-fluoro-7 methylamino-2,4-dioxo-1, 4 -dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt 2.5 hydrate. [0036] Figure 16 provides the DSC data of crystalline solid form B of [4-(6-fluoro-7 5 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt hemi hydrate. [0037] Figure 17 provides the TGA data of crystalline solid form B of [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt. 10 [00381 Figure 18 provides the DSC data of amorphous form of [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea sodium salt. [00391 Figure 19 provides the TGA data of amorphous form of [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 15 sulfonylurea sodium salt. [00401 Figure 20a shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt (form C). Figure 20b shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt (form C). 20 [00411 Figure 21 provides the VT XRPD experiment of [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt (form C). Form C was shown to desolvate to an amorphous phase. [00421 Figure 22 provides the 'H NMR of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt 25 (form C). The NMR confirmed that the only solvent present in the sample was water and it was therefore concluded to have 3.66 moles of water from the TGA weight loss (the NMR was run in DMSO, therefore the signal could not be used to quantify solvent content). A VT XRPD experiment was also carried out to observe if there was an anhydrous form of [4-(6 fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro 30 thiophen-2-yl-sulfonylurea potassium salt tri hydrate (Figure 21). 8 WO 2008/137809 PCT/US2008/062584 [00431 Figure 23 provides the gravimetric vapour sorption (GVS) of [4-(6-fluoro-7 methylamino-2,4-dioxo- 1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt tri hydrate (form C). Form C showed low uptake from 40%RH to 90% RH (ca. I wt %). However, the desorption cycle showed that when dried to 0%RH, the 5 samply lost ca. 8wt% of its mass and when the humidity was then increased to 40%RH the sample did not hydrate to the same level as the input material. [0044] Figure 24 provides the XRPD of [4-(6-fluoro-7-methylamino-2,4-dioxo- 1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt tri hydrate (form C) re-analysis post GVS. The analysis showed the sample to be reduced in 10 crystallinity after the GVS experiment, with some subtle changes in form. [0045] Figure 25 shows the DSC and TGA data of [4-(6-fluoro-7-methylamino-2,4-dioxo 1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt tri hydrate form C. The DSC experiment showed an endotherm of 267Jg' at endotherm onset 56'C associated with a weight loss in the TGA of 10.5w%. 15 [0046] Figure 26 provides the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt (form D). [00471 Figure 27 shows the stability with respect to 40'C/75%RH of [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 20 sulfonylurea potassium salt (form D) by XRPD. The solid converts to an amorphous phase on storage. [00481 Figure 28 provides the 'H NMR spectrum for the potassium salt. [0049] Figure 29 provides the DSC and TGA data of [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea 25 potassium salt (form D). The first two weight losses are likely due to the loss of solvent (THF, IPA and water). [0050] Figure 30 shows the XRPD of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt (form A). [0051] Figure 31 shows the stability with respect to 40'C/75%RH of [4-(6-fluoro-7 30 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 9 WO 2008/137809 PCT/US2008/062584 sulfonylurea sodium salt (form A) by XRPD. The sample was amorphous after the first 3 days of the study, and remained amorphous for the next 4 days of the study. [00521 Figure 32 shows the IH NMR spectrum for the sodium salt. [00531 Figure 33 shows the TGA (green trace) and DSC (blue trace) for form A of the 5 sodium salt. [0054] Figure 34 shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt (form B). [0055] Figure 35 shows the XRPD of Na salt forn B. [00561 Figure 36 shows TGA trace for Form B of the sodium salt. 10 [0057] Figure 37 shows the GVS of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt (form C). [0058] Figure 38 shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea calcium salt (form A). [00591 Figure 39 shows the stability of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4 15 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea calcium salt (form A) by XRPD. The sample remains stable after 3 days at 40'C/75%RH, and a further 4 days at 60 0 C/75%RH. [0060] Figure 40 shows the 'H NMR spectrum for form A of the calcium salt. [00611 Figure 41 shows the GVS of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 20 2 H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea calcium salt (form A). [0062] Figure 42 shows the TGA (green trace) and DSC (blue trace) for form A of the calcium salt. [0063] Figure 43 shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo- 1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea tromethamine salt (form 25 A). [00641 Figure 44 shows the stability of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea tromethamine salt (form A) by XRPD. The sample shows some changes after 3 days at 40'C/75%RH, but no further changes after 4 days at 60'C/75%RH. 10 WO 2008/137809 PCT/US2008/062584 [0065] Figure 45 shows the 'H NMR spectrum for form A of the tromethamine salt. [00661 Figure 46 shows the GVS of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]- 5 -chloro-thiophen-2-yl-sulfonylurea tromethamine salt (form A). 5 [00671 Figure 47 shows the TGA (green trace) and DSC (blue trace) for the tromethamine salt form A. [00681 Figure 48 shows the XRPD of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea ammonium salt (form A). [0069] Figure 49 shows the stability of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4 10 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt (form A) by XRPD. The black diffractogram is the dry ammonium salt Form A and the red trace is the sample after 3 days at 40'C/75%RH and the blue trace is after a further 10 days at 60'C/75%RH. [0070] Figure 50 shows the 'H NMR spectrum for form A of the hemi ammonium salt. 15 [0071] Figure 51 shows the GVS of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt (form A). [0072] Figure 52 show the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt (form 20 A) by XRPD. The black diffractogram is the dry hemi ammonium salf form A and the red trace is the sample after the GVS experiment. [0073] Figure 53 shows the TGA (green trace) and DSC (blue trace) for form A of the hemi ammonium salt form A [00741 Figure 54 shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo- 1,4-dihydro 25 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt (form B) [0075] Figure 55 shows the stability of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea ammonium salt (form B) by XRPD. The black trace is the dry sample and the red trace is the sample after 10 30 days at 60'C/75%RH. 11 WO 2008/137809 PCT/US2008/062584 [00761 Figure 56 shows the 'H NMR spectrum for form B of the hemi ammonium salt. [00771 Figure 57 shows the GVS of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt (form B). 5 [0078] Figure 58 shows the TGA (green trace) and DSC (blue trace) for form B of the hemi ammonium salt. [0079] Figure 59 shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea L-lysine salt monohydrate (form A). 10 [0080] Figure 60 shows the 'H NMR spectrum for the amorphous L-lysine salt [0081] Figure 61 shows the XRPD of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea magnesium salt (form A). [0082] Figure 62 shows the 'H NMR spectrum for form A of the magnesium salt. [0083] Figure 63 shows the TGA trace for form A of the magnesium salt. 15 [00841 Figure 64 shows three XRPD of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea L-arginine salts (amorphous form): the black diffractogram is made from L-arginine in acetonitrile/water, the red trace is made from L-arginine in iso-propyl alcohol and the blue diffractogram is made from L-arginine in water. 20 [0085] Figure 65 the 'H NMR spectrum for amorphous form of the L-arginine salt from acetonitrile/water. [0086] Figure 66 shows the XRPD of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea N-ethylglucamine salt (amorphous form) from acetonitrile/water. 25 [0087] Figure 67 shows the XRPD of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea N-methylglucamine salt (amorphous form) from THF. [0088] Figure 68 shows the 'H NMR spectrum for amorphous form of the N methylglucamine salt from THF. 12 WO 2008/137809 PCT/US2008/062584 DETAILED DESCRIPTION OF THE INVENTION [0089] The present invention involves sulfonylurea compounds and their derivatives and crystalline solid and amorphous forms thereof, and their preparation. A selection of salts of 5 [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea have been isolated as crystalline solids of high purity. The salts of the present invention are useful for the treatment and prevention of undesired thrombosis and thrombosis related conditions in mammals. 10 I. Definitions [0090] In accordance with the present invention and as used herein, the following terms are defined with the following meanings, unless explicitly stated otherwise. [0091] The phrase "a" or "an" entity as used herein refers to one or more of that entity; for example, a compound refers to one or more compounds or at least one compound. As such, 15 the terms "a" (or "an"), "one or more", and "at least one" can be used interchangeably herein. [0092] The phrase "about" as used herein means variation one might see in measurements taken among different instruments, samples, and sample preparations. Such variation may include, for instance, colligative properties for thermal measurements. Typical variation among different X-ray diffractometers and sample preparations for crystalline solid forms is 20 on the order of 0.2 020. Typical variation for Raman and IR spectrometers is on the order of twice the resolution of the spectrometer. The resolution of the spectrometer used was about 2 cm [0093] The term "solvate" as used herein means a compound of the invention or a salt, thereof, that further includes a stoichiometric or non-stoichiometric amount of a solvent 25 which forms part of the crystal lattice by either non-covalent binding or by occupying a hole in the crystal lattice. [0094] The term "hydrate" as used herein means a compound of the invention or a salt thereof, that further includes a stoichiometric or non-stoichiometric amount of water which forms part of the crystal lattice by either non-covalent bonding or by occupying a hole in the 30 crystal lattice. Hydrates are formed by the combination of one or more molecules of water 13 WO 2008/137809 PCT/US2008/062584 with one of the substances in which the water retains its molecular state as H 2 0, such combination being able to form one or more hydrates. [0095] The term "anhydrous" as used herein means a compound of the invention or a salt thereof that does not contain solvent in the crystal lattice. 5 [00961 The term "drying" as used herein means a method of removing solvent and/or water from a compound of the invention which, unless otherwise specified, may be done at atmospheric pressure or under reduced pressure and with or without heating until the level of solvent and/or water contained reached an acceptable level. [00971 The term "polymorphs" as used herein means crystal structures in which a 10 compound can crystallize in different crystal packing arrangements, all of which have the same elemental composition. Different crystal forms can have different X-ray diffraction patterns, infrared spectra, melting points/endotherm onset and maximums, density hardness, crystal shape, optical and electrical properties, stability and solubility. Recrystallization solvent, rate of crystallization, storage temperature, and other factors may effect which 15 crystal form is generated. [00981 The term "solid form" as used herein means crystal structures in which compounds can crystallize in different packing arrangements. Solid forms include polymorphs, hydrates, and solvates as those terms are used in this invention. Different solid forms, including different polymorphs, of the same compound may exhibit different x-ray powder diffraction 20 patterns and different spectra including infra-red, Raman, DSC and solid-state NMR. Their optical, electrical, stability, and solubility properties may also differ. [00991 The term "characterize" as used herein means to select data from an analytical measurement such as X-ray powder diffraction, DSC, infra-red spectroscopy, Raman spectroscopy, and/or solid-state NMR to distinguish one solid form of a compound from 25 other solid forms of a compound. [0100] The term "mammal" includes, without limitation, humans, domestic animals (e.g., dogs or cats), farm animals (cows, horses, or pigs), monkeys, rabbits, mice, and laboratory animals. [0101] The term "alkyl" refers to saturated aliphatic groups including straight-chain, 30 branched-chain and cyclic groups having the number of carbon atoms specified, or if no number is specified, having up to about 12 carbon atoms. Examples of alkyl groups include 14 WO 2008/137809 PCT/US2008/062584 methyl, ethyl, n-propyl, isopropyl, n-butyl, t-butyl, isobutyl, sec-butyl, n-pentyl, n-hexyl, n heptyl, n-octyl, and the like. [0102] The terms "alkoxy," "alkylamino" and "alkylthio" (or thioalkoxy) are used in their conventional sense, and refer to those alkyl groups attached to the remainder of the molecule 5 via an oxygen atom, an amino group, or a sulfur atom, respectively. For brevity, the term C 1 . 6 alkylamino is meant to include straight chain, branched or cyclic alkyl groups or combinations thereof, such as methyl, ethyl, 2-methylpropyl, cyclobutyl and cyclopropylmethyl. [0103] The term "C 1

-C

6 alkylamino" or "CI- 6 alkylamino" as used herein refers to an amino 10 moiety attached to the remainder of the molecule whereby the nitrogen is substituted with one or two C 1

-

6 alkyl substituents, as defined above. [01041 The terms "halo" or "halogen," by themselves or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom. Additionally, terms such as "haloalkyl," are meant to include monohaloalkyl and polyhaloalkyl. For 15 example, the term "C 1

-

4 haloalkyl" is mean to include trifluoromethyl, 2,2,2-trifluoroethyl, 4 chlorobutyl, 3-bromopropyl, and the like. [0105] The term "pharmaceutically acceptable derivatives" is meant to include salts of the active compounds which are prepared with relatively non-toxic acids or bases, depending on the particular substituents found on the compounds described herein. When compounds of 20 the present invention contain relatively acidic functionalities, base addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired base, either neat or in a suitable inert solvent. Examples of pharmaceutically acceptable base addition salts include those derived from inorganic bases such as sodium, potassium, lithium, ammonium, calcium, magnesium, iron, zinc, copper, manganese, 25 aluminum salts and the like. Particularly preferred are the potassium, sodium, calcium, ammonium and magnesium salts. Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, 30 ethanolamine, 2-diethylaminoethanol, tromethamine, trimetharnine, dicyclohexylamine, caffeine, procaine, hydrabamine, choline, betaine, ethylenediamine, glucosamine, N ethylglucamine, N-methylglucamine, theobromine, purines, piperazine, piperidine, N 15 WO 2008/137809 PCT/US2008/062584 ethylpiperidine, polyamine resins, amino acids such as lysine, arginine, histidine, and the like. Particularly preferred organic non-toxic bases are L-amino acids, such as L-lysine and L arginine, tromethamine, N-ethylglucamine and N-methylglucamine. When compounds of the present invention contain relatively basic functionalities, acid addition salts can be obtained 5 by contacting the neutral form of such compounds with a sufficient amount of the desired acid, either neat or in a suitable inert solvent. Examples of pharmaceutically acceptable acid addition salts include those derived from inorganic acids like hydrochloric, hydrobromic, nitric, carbonic, monohydrogencarbonic, phosphoric, monohydrogenphosphoric, dihydrogenphosphoric, sulfuric, monohydrogensulfuric, hydriodic, or phosphorous acids and 10 the like, as well as the salts derived from relatively non-toxic organic acids like acetic, propionic, isobutyric, malonic, benzoic, succinic, suberic, fumaric, mandelic, phthalic, benzenesulfonic, p-tolylsulfonic, citric, tartaric, methanesulfonic, and the like. Also included are salts of amino acids such as arginate and the like, and salts of organic acids like glucuronic or galactunoric acids and the like (see, for example, Berge, S.M., et al, 15 "Pharmaceutical Salts", Journal ofPharmaceutical Science, 1977, 66, 1-19; Bundgaard, H., ed., Design ofProdrugs (Elsevier Science Publishers, Amsterdam 1985)). Certain specific compounds of the present invention contain both basic and acidic functionalities that allow the compounds to be converted into either base or acid addition salts. [01061 The neutral forms of the compounds may be regenerated by contacting the salt with 20 a base or acid and isolating the parent compound in the conventional manner. The parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the present invention. [0107] In addition to salt forms, the term "pharmaceutically acceptable derivatives" is 25 meant to include compounds which are in a prodrug form. "Prodrugs" of the compounds described herein are those compounds that readily undergo chemical changes under physiological conditions to provide the compounds of the present invention. Additionally, prodrugs can be converted to the compounds of the present invention by chemical or biochemical methods in an ex vivo environment. For example, prodrugs can be slowly 30 converted to the compounds of the present invention when placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent (see Bundgaard, H., ed., Design of Prodrugs (Elsevier Science Publishers, Amsterdam 1985)). 16 WO 2008/137809 PCT/US2008/062584 [01081 "Pharmaceutically acceptable ester" refers to those esters which retain, upon hydrolysis of the ester bond, the biological effectiveness and properties of the carboxylic acid or alcohol and are not biologically or otherwise undesirable. For a description of pharmaceutically acceptable esters as prodrugs, see Bundgaard, H., supra. These esters are 5 typically formed from the corresponding carboxylic acid and an alcohol. Generally, ester formation can be accomplished via conventional synthetic techniques. (See, e.g., March Advanced Organic Chemistry, 3rd Ed., p. 1157 (John Wiley & Sons, New York 1985) and references cited therein, and Mark et al., Encyclopedia of Chemical Technology, (1980) John Wiley & Sons, New York). The alcohol component of the ester will generally comprise: (i) a 10 C 2

-C

1 2 aliphatic alcohol that can or can not contain one or more double bonds and can or can not contain branched carbons; or (ii) a C 7

-C

1 2 aromatic or heteroaromatic alcohols. The present invention also contemplates the use of those compositions which are both esters as described herein and at the same time are the pharmaceutically acceptable acid addition salts thereof. 15 [0109] "Pharmaceutically acceptable amide" refers to those amides which retain, upon hydrolysis of the amide bond, the biological effectiveness and properties of the carboxylic acid or amine and are not biologically or otherwise undesirable. For a description of pharmaceutically acceptable amides as prodrugs, see, Bundgaard, H., ed., supra. These amides are typically formed from the corresponding carboxylic acid and an amine. Generally, 20 amide formation can be accomplished via conventional synthetic techniques. See, e.g., March et al., Advanced Organic Chemistry, 3rd Ed., p. 1152 (John Wiley & Sons, New York 1985), and Mark et al., Encyclopedia of Chemical Technology, (John Wiley & Sons, New York 1980). The present invention also contemplates the use of those compositions which are both aides as described herein and at the same time are the pharmaceutically acceptable acid 25 addition salts thereof. [0110] The term "pharmaceutically acceptable derivatives" is also meant to include compounds of the present invention which can exist in unsolvated forms as well as solvated forms, including hydrated forms. In general, the solvated forms are equivalent to unsolvated forms and are intended to be encompassed within the scope of the present invention. Certain 30 compounds of the present invention may exist in multiple crystalline or amorphous forms. In general, all physical forms are equivalent for the uses contemplated by the present invention and are intended to be within the scope of the present invention. 17 WO 2008/137809 PCT/US2008/062584 [0111] Certain compounds of the present invention possess asymmetric carbon atoms (optical centers) or double bonds; the racemates, diastereomers, geometric isomers and individual isomers (e.g., separate enantiomers) are all intended to be encompassed within the scope of the present invention. 5 [01121 The compounds of the present invention may also contain unnatural proportions of atomic isotopes at one or more of the atoms that constitute such compounds. For example, the compounds may be radiolabeled with radioactive isotopes, such as for example tritium (3H), iodine-125 (125I) or carbon-14 (1C). All isotopic variations of the compounds of the present invention, whether radioactive or not, are intended to be encompassed within the 10 scope of the present invention. [0113] "Biological property" for the purposes herein means an in vivo effector or antigenic function or activity that is directly or indirectly performed by a compound of this invention that are often shown by in vitro assays. Effector functions include receptor or ligand binding, any enzyme activity or enzyme modulatory activity, any carrier binding activity, any 15 hormonal activity, any activity in promoting or inhibiting adhesion of cells to an extracellular matrix or cell surface molecules, or any structural role. Antigenic functions include possession of an epitope or antigenic site that is capable of reacting with antibodies raised against it. [0114] The term "treatment" or "treating" means any treatment of a disease or disorder in a 20 subject, such as a mammal, including: preventing or protecting against the disease or disorder, that is, causing the clinical symptoms not to develop; inhibiting the disease or disorder, that is, arresting or suppressing the development of clinical symptoms; and/or 25 relieving the disease or disorder that is, causing the regression of clinical symptoms. [01151 As used herein, the term "preventing" refers to the prophylactic treatment of a patient in need thereof. The prophylactic treatment can be accomplished by providing an appropriate dose of a therapeutic agent to a subject at risk of suffering from an ailment, thereby substantially averting onset of the ailment. 30 [0116] It will be understood by those skilled in the art that in human medicine, it is not always possible to distinguish between "preventing" and "suppressing" since the ultimate 18 WO 2008/137809 PCT/US2008/062584 inductive event or events may be unknown, latent, or the patient is not ascertained until well after the occurrence of the event or events. Therefore, as used herein the term "prophylaxis" is intended as an element of "treatment" to encompass both "preventing" and "suppressing" as defined herein. The term "protection," as used herein, is meant to include "prophylaxis." 5 [01171 The term "therapeutically effective amount" refers to that amount of a salt of this invention, typically delivered as a pharmaceutical composition, that is sufficient to effect treatment, as defined herein, when administered to a subject in need of such treatment. The therapeutically effective amount will vary depending upon the subject and disease condition being treated, the weight and age of the subject, the severity of the disease condition, the 10 particular compound chosen, the dosing regimen to be followed, timing of administration, the manner of administration and the like, all of which can be determined readily by one of ordinary skill in the art. [01181 As used herein, the term "condition" refers to a disease state for which the compounds, compositions and methods of the present invention are being used against. 15 [0119] As used herein, the term " ADP -mediated disease or condition" and the like refers to a disease or condition characterized by less than or greater than normal, ADP activity. A ADP -mediated disease or condition is one in which modulation of ADP results in some effect on the underlying condition or disease (e.g., a ADP inhibitor or antagonist results in some improvement in patient well-being in at least some patients). 20 [01201 As used herein, the term "blood sample" refers to whole blood taken from a subject, or any fractions of blood including plasma or serum. [01211 In the compounds of this invention, carbon atoms bonded to four non-identical substituents are asymmetric. Accordingly, the compounds may exist as diastereoisomers, enantiomers or mixtures thereof. The syntheses described herein may employ racemates, 25 enantiomers or diastereomers as starting materials or intermediates. Diastereomeric products resulting from such syntheses may be separated by chromatographic or crystallization methods, or by other methods known in the art. Likewise, enantiomeric product mixtures may be separated using the same techniques or by other methods known in the art. Each of the asymmetric carbon atoms, when present in the compounds of this invention, may be in one of 30 two configurations (R or S) and both are within the scope of the present invention. 19 WO 2008/137809 PCT/US2008/062584 II. Free Acid Compounds [0122] Compounds of formula (II) include the compound having the formula: F HN \ 0 0 C H NC HN H ON 5 III. Preparation of Free Acid Compounds [0123] Scheme 1 illustrates a method of preparing certain compounds of formulas I and II wherein Ar is phenylene. SCHEME 1 0 0 F O-CH

H

2 F O H3CHN NO 2 Pd/C or H 3 CHN NH 2 Pt(S)/C 2 F 0 0

CH

2 1 NMM jOr Method A or B o PS-NMM N H3CHN NH NH-Ar-NO 2 ornone H3CHN& N rI1 90 0 c H 0 0a 4a ci 3a 0 H H

H

2 Fe N Ar. r- N N H2N' N H 2 F N Yr fS H Coupling N i 00 H-1CHN N-'0 Pd/C or H HHCHN N0 Pt(S)/C 5a H i4 10 [0124] A compound of formula 11 can be prepared by reducing 2-nitro-benzoic acid methyl ester compound 1 by procedures known to one skilled in the art to yield aniline 2. (See also published patent application US 2002/077486). For example, a method of nitro group reduction can be carried out by hydrogenation. The hydrogenation is carried out with a 15 suitable catalyst (e.g., 10% Pd/C or Pt(s)/C) under hydrogen and in an appropriate solvent, typically in an alcohol, preferably ethanol at room temperature. Treating compound 2 with appropriately substituted aryl isocyanate (Method A) provides intermediate urea 3a. Alternatively, urea 3a can be formed by treating compound 2 with triphosgene in the presence of a base such as triethylamine or diisopropylethylamine in an inert solvent such as 20 WO 2008/137809 PCT/US2008/062584 THF, dichloromethane and MeCN at appropriate temperature, preferably at 20 C, followed by substituted aniline (Method B). Urea 3a, prepared by Method A or Method B typically without further purification can be subjected to thermal or base (such as N-methyl morpholine (NMM) or polystyrene-NMM (PS-NMM) induced ring closure to provide 5 quinazolinedione 4a. The nitro group of compound 4a can be reduced by procedures known to one skilled in the art to yield free amino group. For example, a method of reduction can be carried out by hydrogenation, with a suitable catalyst (e.g., 10% palladium on carbon) in an appropriate solvent, typically an alcohol. The formation of sulfonylurea linkage can be accomplished by treating the reduced product aniline 5a with a pre-mixed solution of 10 substituted thiophene-2-sulfonamide, N, N'-disuccinimidyl carbonate and tetramethylguanidine in dichloromethane, followed by treatment with TFA in dichloromethane at room temperature to afford the sulfonylurea of formula II. Alternatively, the sulfonylurea linkage can be formed by reacting the aniline 5a and 5-Chloro-thiophene-2 sulfonyl ethylcarbamate in suitable solvents, which include, but are not limited to, toluene, 15 acetonitrile, 1,4-dioxane and DMSO. [01251 Scheme 2 illustrates an alternative method of preparing compounds of Formula II wherein for example L'is halogen, alkylsulfonate, haloalkylsulfonate and arylsulfonate. 21 WO 2008/137809 PCT/US2008/062584 SCHEME 2 F CO 2 Me Method B F CO 2 Me I H H ANHBoc Li NH 2 L1 N -r N- r 2 3b 0 0 NaOMe, MeOH F NAr'NH Boc HCI/dioxane L' N0 H 4b 0 0 F A r-NH2.HCI MeNH 2 , DMSO F N .Ar'NH2.HCI 5H 100 0 C H 3 CN 5cH R2

R

2 N O N CflN--'; -, 1/ H 0 F 2NMOH H3 N N 0 HHN R2 H HH FO N N F O N 00 SN0

H

3 CN N OIa [0126] The urea 3b can be prepared by treating compound 2 with triphosgene or p nitrophenyl chloroformate in the presence of a base, such as triethylamine and/or 5 diisopropylethylamine, in an inert solvent, such as THF, dichloromethane and/or MeCN, at an appropriate temperature, typically at about 20 *C, followed by treatment with an appropriately protected aniline (Method B). Urea 3b, typically without further purification, can be subjected to base induced ring closure to provide intermediate quinazolinedione 4b. The protecting group of compound 4b can be removed using standard techniques appropriate 10 for the protecting group used. For example a BOC protecting group can be removed by treating compound 4b with 4N HCl in dioxane. The C-7 fluoro of compound 5b is then displaced by treatment with methylamine in DMSO at about 120 C to afford aniline 5c. The preparation of target sulfonylurea 11 can be accomplished by treating aniline 5c with 5 22 WO 2008/137809 PCT/US2008/062584 chloro-thiophene-2-sulfonyl ethylcarbamate in an appropriate solvent, such as dimethyl sulfoxide, dioxane and/or acetonitrile with heating. Treatment of a compound of the invention with an acid or base may form, respectively, a pharmaceutically acceptable acid addition salt and a pharmaceutically acceptable base addition salt, each as defined herein. 5 Various inorganic and organic acids and bases known in the art including those defined herein may be used to effect the conversion to the salt. [0127] Scheme 3 illustrates an alternative method of preparing compounds of Formula II wherein for example L'is halogen, alkylsulfonate, haloalkylsulfonate and arylsulfonate and M is K. 23 WO 2008/137809 PCT/US2008/062584 SCHEME 3 0 NO 2 F OMe CI 0 5 (1.2 equiv) F 0 OMe O~e

C

7

H

4

CINO

4 K-MoI. W.: 201.66 Ll NHN0 L1 NH 2 p-nitrophenylchloroformate O O Step 1 2 3a H 0 NF OMe NHBoc

H

2 N / O NH NHBoc F 0NF N tert-butyl 4-aminophenylcarbamate H N O H Step 2 3b Boc= 0 4b H N-O N F OH 2 0 NH0 0 0 (2.0 equiv) F N H 3

NH

2 (7 equiv) F Nj 11DMVSO ethyl 5-chlorothiophen-2 N 0 H 3 CHN N~ -'-ylsulfonylcarbamate H Step3 H ISOA DMSO, A Step4 5b 5c H H H S O N N 2N MOH(1.15 equiv) O NN F O N 0 ACN/H 2 0 F O N 00

H

3 CHN H Step 5 N H [01281 The quinazolinedione 5b can be prepared by treating compound 2 with p nitrophenylchloroformate, in an inert solvent, such as THF, dichloromethane and/or MeCN, 5 at an appropriate temperature, typically at about 20 0 C, followed by treatment with an appropriately protected aniline (Method B). The C-7 fluoro of compound 5b is then displaced by treatment with methylamine in DMSO at about 120 0 C to afford aniline 5c. The preparation of target sulfonylurea II can be accomplished by treating aniline 5c with 5 chloro-thiophene-2-sulfonyl ethylcarbamate in an appropriate solvent, such as dimethyl 24 WO 2008/137809 PCT/US2008/062584 sulfoxide, dioxane and/or acetonitrile with heating. According to the invention, compounds of formula (I) may be further treated to form pharmaceutically acceptable salts e.g. I. Treatment of a compound of the invention with an acid or base may form, respectively, a pharmaceutically acceptable acid addition salt and a pharmaceutically acceptable base 5 addition salt, each as defined above. Various inorganic and organic acids and bases known in the art including those defined herein may be used to effect the conversion to the salt. [0129] Compounds of formula II may be isolated using typical isolation and purification techniques known in the art, including, for example, chromatographic and recrystallization methods. 10 IV. Preparation of the Salts of Formula I [0130] According to one embodiment of the invention, compounds of formula II may be further treated to form pharmaceutically acceptable salts. Treatment of a compound of the invention with an acid or base may form, respectively, a pharmaceutically acceptable acid 15 addition salt and a pharmaceutically acceptable base addition salt, each as defined above. These salts will preferably provide the requisite crystallinity, thermal, hydrolytic and hygroscopic stability and purity. Various inorganic and organic acids and bases known in the art including those defined herein may be used to effect the conversion to the salt. In one embodiment, the salts include but are not limited to, sodium and potassium salts. In another 20 embodiment, the salts include but are not limited to, calcium, L-lysine, ammonium, magnesium, L-arginine, tromethamine, N-ethylglucamine and N-methylglucamine salts. One of skill in the art will recognize that other bases can be used to make salts comprising the compound of Formula I that are useful in the present invention. It is also contemplated that salts of the invention can be readily converted to other salts of the invention. 25 [0131] To assess the thermal and hydrolytic stability of the salt, tests known to those of skill in the art are performed. These tests are more thoroughly discussed below. [0132] A number of methods are useful for the preparation of the salts described above and are known to those skilled in the art. For example, reaction of the compound of Formula II with one or more molar equivalents of the desired base in a solvent or solvent mixture in 30 which the salt is insoluble, or in a solvent like water after which the solvent is removed by evaporation, distillation or freeze drying. Alternatively, the compound of Formula II may be 25 WO 2008/137809 PCT/US2008/062584 passed over an ion exchange resin to form the desired salt or one salt form of the product may be converted to another using the same general process. [0133] The salts of Formula I can be prepared according to any of several different methodologies, either on a gram scale (< 1 kg) or a kilogram scale (> 1 kg). 5 [0134] A variety of solvents can be used for the method of the present invention as described above including but not limited to a non-polar, aprotic solvent such as tetrahydrofuran (THF), diethyl ether, dimethoxymethane, dioxane, hexane, methyl tert-butyl ether, heptane, and cyclohexane. In addition, the formation of the urea can be carried at temperatures below 10 'C. One of skill in the art will recognize that the methods of the 10 present invention can be practiced using various other solvents, reagents, and reaction temperatures. [01351 The salts of Formula I can be prepared using the method of the present invention in yields greater than 50%. In some instances, the compound of Formula I can be prepared in yields greater than 65%. In other instances, the compound of Formula I can be prepared in 15 yields greater than 75%. One of skill in the art will recognize that the salts of Formula I can be prepared via other chemical methodologies on both a gram and kilogram scale. [0136] The invention also provides pharmaceutically acceptable isomers, hydrates, and solvates of compounds of formula (I). Compounds of formula (I) may also exist in various isomeric and tautomeric forms including pharmaceutically acceptable salts, hydrates and 20 solvates of such isomers and tautomers. For example, while some compounds are provided herein as dihydrates having two molecules of water per molecule of the compound of formula II, the present invention also provides compounds that are anhydrous, hemihydrates, monohydrates, trihydrates, sesquihydrates, and the like. 25 IV. Crystalline solid and Amorphous Embodiments of the Invention and their Preparation [0137] The present invention also provides crystalline solid and/or amorphous salts of [4 (6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea and processes for their preparation and pharmaceutical 30 compositions comprising these forms. The salts have the following general formula: 26 WO 2008/137809 PCT/US2008/062584 F HN0 0c+I HC NHN HN NN 0/0 0 wherein M is an ion selected from the group consisting of: calcium, L-lysine, ammonium, magnesium, L-arginine, tromethamine, N-ethylglucamine and N-methylglucamine. In other embodiments, M is selected from sodium or potassium. The different crystalline forms of the 5 same compound can have an impact on one or more physical properties, such as stability, solubility, melting point, bulk density, flow properties, bioavailability, etc. [01381 In developing a process for production of an active pharmaceutical ingredient (API), two factors are of great importance: the impurity profile and the crystal morphology of the compound. The results from the initial isolation and crystallization work showed a profile of 10 [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea of 99.6%. Preferably the API has levels of impurities below 0.2% and is in the most thermodynamically stable crystalline solid form. The isolation and crystallization work indicated that there was an amorphous phase and at least four crystalline solid forms of the potassium salt of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H 15 quinazolin- 3 -yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea (designated as form A, B, C and D), an amorphous phase and at least three crystalline solid forms of the sodium salt of [4 (6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea (designated as form A, B and C), at least two crystalline solid forms of the calcium salt of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H 20 quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea (designated as form A and B), at least two crystalline solid forms of the ammonium salt of [ 4 -(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea (designated as form A and B), at least one solid form of the L-lysine salt of [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 25 sulfonylurea (designated as form A), at least one crystalline solid forms of the magnesium salt of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5 chloro-thiophen-2-yl-sulfonylurea (designated as form A), at least one crystalline solid forms of the tromethamine salt of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea (designated as form A), and at 27 WO 2008/137809 PCT/US2008/062584 least one amorphous form of the L-arginine salt, the N-ethylglucamine salt and the N methylglucamine salt of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin 3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea. [0139] The solid forms of the invention may be described by one or more of several 5 techniques including X-ray powder diffraction, Raman spectroscopy, IR spectroscopy, and thermal methods. Further, combinations of such techniques may be used to describe the invention. For example, one or more X-ray powder diffraction patterns combined with one or more Raman spectrum may be used to describe one or more solid forms of the invention in a way that differentiates it from the other solid forms. 10 [01401 Although it characterizes a form, it is not necessary to rely only upon an entire diffraction pattern or spectrum to characterize a solid form. Those of ordinary skill in the pharmaceutical arts recognize that a subset of a diffraction pattern or spectrum may be used to characterize a solid form provided that subset distinguishes the solid form from the other forms being characterized. Thus, one or more X-ray powder diffraction pattern alone may be 15 used to characterize a solid form. Likewise, one or more IR spectrum alone or Raman spectrum alone may be used to characterize a solid form. Such characterizations are done by comparing the X-ray, Raman, and IR data amongst the forms to determine characteristic peaks. [01411 One may also combine data from other techniques in such a characterization. Thus, 20 one may rely upon one or more x-ray powder diffraction pattern and for example, Raman or IR data, to characterize a form. For example, if one or more X-ray diffraction peak characterize a form, one could also consider Raman or IR data to characterize the form. It is sometimes helpful to consider Raman data, for example, in pharmaceutical formulations. [0142] The polymorphs were isolated by using different crystallization conditions. For the 25 potassium salt, (1) crystalline form A was isolated after crystallization of the crude wet-cake from methanol and drying the crude wet-cake to effect solvent removal, (2) crystalline solid form B was formed from crystallization from EtOH/H 2 0 or by trituration with methanol, (3) crystalline solid form C was formed through grinding or suspending form B in water, or by suspending the amorphous potassium salt in water at ambient conditions it converted to form 30 C within 16 hours. Form D could also be formed from crystallization from KOH in THF. [0143] The potassium salt was suspended in methanol and then heated until a clear solution was observed. This was followed by cooling and the resulting crystalline solid was isolated 28 WO 2008/137809 PCT/US2008/062584 and dried at room temperature under reduced pressure to give crystalline solid potassium salt form A. Form A is a mono potassium salt 2.5 hydrate. Form B is a mono potassium salt hemi hydrate. Figures 14 and 2 respectively show the DSC trace and the X-ray powder pattern for the crystalline solid form A. Differential scanning calorimetry (DSC) of form A 5 of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea potassium salt defined a melt of dehydrated salt at 238 C. A large decomposition peak was recorded, onset temperature approximately 300 0 C. [01441 In the X-ray powder diffraction pattern, the peaks at about 9.5 and 25.5 are the main features of the pattern (for a discussion of the theory of X-ray powder diffraction patterns see 10 "X-ray diffraction procedures" by H. P. Klug and L. E. Alexander, J. Wiley, New York (1974)). The peaks at about 9.5' 20 and 25.50 20 characterize form A with respect to form B because form B does not have peaks to within 0.20 20, twice the approximate precision of X ray powder diffraction peaks, of the two form A peaks. Because the typical variation in any given X-ray powder diffraction peak is on the order of 0.2' 20, when selecting peaks to 15 characterize a polymorph, one selects peaks that are at least twice that value (i.e., 0.40) from a peak from another polymorph. Thus, in a particular polymorph X-ray pattern, a peak that is at least 0.40 from a peak in another polymorph is eligible to be considered as a peak that can either alone or together with another peak be used to characterize that polymorph. Tables 1 and 2 identify the main peaks of forms A and B. From that list, one sees that the peak at 20 about 25.50 20 (on the table listed as 25.478 20), when taken to one decimal point, is greater than 0.20 20 away from any peak in forms B. Thus, the peak at about 25.50 20 can be used to distinguish form A from form B. The peak at about 9.5' 20 (9.522 '20 in Table 1) is the most intense peak in the form A X-ray powder diffraction pattern of Figure 2 and is more than 0.2 020 away from any peak in form B. Thus, the form A peaks at about 9.5'20 and 25 25.5 020 characterize form A with respect to form B. The solid form isolated at this stage in the process contained about 2.5 molecules of water to one molecule of salt. Table 1 Potassium Salt form A XRPD Peak (020) and % Intensity Listing Data Tabulated from Figure 2b. 30 Intensity (%) Angle (*2-Theta) d value (A) 100.0 9.522 9.28049 35.0 25.478 3.49317 24.2 28.764 3.10110 29 WO 2008/137809 PCT/US2008/062584 22.5 27.175 3.27877 20.1 19.090 4.64529 15.2 22.977 3.86744 14.4 24.630 3.61155 13.8 23.987 3.70680 12.3 15.530 5.70104 12.3 18.518 4.78751 12.1 18.146 4.88482 9.5 16.223 5.45912 8.9 13.219 6.69229 8.7 21.040 4.21883 6.8 16.929 5.23304 5.6 4.822 18.31110 Table 2 Potassium Salt form B XRPD Peak (020) and % Intensity Listing Data Tabulated from Figure 3b. Intensity (%) Angle (*2-Theta) d value (A) 100.0 25.087 3.54667 70.4 20.328 4.36505 63.9 24.442 3.63878 52.9 5.339 16.53922 50.9 19.594 4.52687 34.7 26.155 3.40428 30.6 17.37 5.10115 28.6 21.373 4.15387 28.1 14.526 6.09284 27.6 22.53 3.94319 26.5 9.921 8.90794 26.5 21.729 4.08664 24.9 13.569 6.52011 23.6 15.346 5.76906 22.9 29.478 3.02760 18.9 10.655 8.29583 5 [0145] Preferred orientation can affect peak intensities, and in some cases peak positions, in XRPD patterns. In the case of the potassium salts, preferred orientation has the most noticeable effect at lower angles. Preferred orientation causes some peaks in this region to be diminished (or increased). Crystal habit does not clearly differentiate between the solid forms; a variety of habits have been observed for each form, including needles, blades, plates, 10 and irregular-shaped particles. [0146] Figures 16 and 3 respectively show the DSC trace and the X-ray powder pattern for another crystalline solid. These results were observed when the remaining water was removed. In the DSC trace, an endotherm onset at about 286 C is noteworthy, because the 30 WO 2008/137809 PCT/US2008/062584 dehydrated form A melts at 246 C. The peaks at about 20.3 020 and 25.1 020 in the X-ray powder diffraction pattern also characterize form B with respect to form A, because form A does not have peaks to within 0.2' 20, the approximate precision of X-ray powder diffraction peaks, of the two characteristic form B peaks (see Tables 1 and 2). From that list, one sees 5 that the peaks at about 20.3'20 and 25.1 '20 (in Table 2 listed as 20.328 020 and 25.087 20, respectively), when taken to one decimal point, is greater than 0.20 20 away from any peak in form A. Thus, the peaks at about 20.3020 and 25.1 020 can be used to distinguish form B from form A. 10 Potassium Salt Form C and D [01471 Figures 25 and 20 respectively show the DSC trace and the X-ray powder pattern for another crystalline solid form C. In the DSC trace, an endotherm onset at about 56 0 C is noteworthy. [01481 Figures 29 and 26-27 respectively show the DSC trace and the X-ray powder pattern 15 for another crystalline solid form D. In the DSC trace, an endotherm onset at about 132 C is noteworthy. [01491 Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo- 1, 4 -dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt in new crystalline forms designated as form C and form D. 20 [01501 Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt in a crystalline solid form, including a substantially pure form, which provides at least one of: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 26 or 27 and 25 (ii) a DSC scan substantially in accordance with FIG. 29; herein designated as form D. [01511 In another embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea 31 WO 2008/137809 PCT/US2008/062584 potassium salt in a crystalline solid form, including a substantially pure form, which provides a DSC endotherm onset at about 56 'C; herein designated as form C. [0152] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea 5 potassium salt in a crystalline solid form, including a substantially pure form, which provides at least one of: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 20b; and (ii) a DSC scan substantially in accordance with FIG. 25; herein designated as form C. [0153] In another embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 10 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt in a crystalline solid form, including a substantially pure form, which provides a DSC endotherm onset at about 132 'C; herein designated as form D. [01541 In another embodiment the present invention provides [4-(6-fluoro-7-methylamino 2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea 15 potassium salt in an amorphous form. Sodium Salt Form A, B and C [0155] Figures 33 and 30 respectively show the DSC trace and the X-ray powder pattern for another crystalline solid form A. In the DSC trace, an endotherm onset at about 162 0 C is 20 noteworthy. [01561 Figures 36 shows the X-ray powder pattern for another crystalline solid form B. [0157] Figure 20a shows the X-ray powder pattern for another crystalline solid form C. [0158] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 25 sulfonylurea sodium salt in new crystalline forms designated as form A, form B and form C. [0159] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt in a crystalline solid form, including a substantially pure form, which provides at least one of: 32 WO 2008/137809 PCT/US2008/062584 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 30; and (ii) a DSC scan substantially in accordance with FIG. 33; herein designated as form A. [0160] In another embodiment, the invention provides [ 4

-(

6 -fluoro-7-methylamino-2,4 5 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt in a crystalline solid form, including a substantially pure form, which provides a DSC endotherm onset at about 162 C; herein designated as form A. [01611 Thus in one embodiment, the invention provides [ 4

-(

6 -fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium 10 salt in a crystalline solid form, including a substantially pure form, which provides: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 36. [0162] Thus in one embodiment, the invention provides [ 4

-(

6 -fluoro-7-methylamino-2,4 dioxo-1, 4 -dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt in a crystalline solid form, including a substantially pure form, which provides at least 15 one of: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 20a; herein designated as form C. [01631 In another embodiment, the invention provides [ 4

-(

6 -fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium 20 salt in a crystalline solid form, including a substantially pure form, which provides a DSC endotherm onset at about 80 C; herein designated as form C. Calcium Salt Form A [0164] Figures 42 and 38 respectively show the DSC trace and the X-ray powder pattern 25 for another crystalline solid form A. In the DSC trace, an endotherm onset at about 125C is noteworthy. [0165] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea calcium salt in new crystalline forms designated as form A. 33 WO 2008/137809 PCT/US2008/062584 [0166] Thus in one embodiment, the invention provides [ 4 -(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea calcium salt in a crystalline solid form, including a substantially pure form, which provides at least one of: 5 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 38; and (ii) a DSC scan substantially in accordance with FIG. 42; herein designated as form A. [0167] In another embodiment, the invention provides [ 4 -(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea calcium 10 salt in a crystalline solid form, including a substantially pure form, which provides a DSC endotherm onset at about 125 'C; herein designated as form A. Tromethamine Salt Form A [0168] Figures 47 and 43 respectively show the DSC trace and the X-ray powder pattern 15 for another crystalline solid form A. In the DSC trace, an endotherm onset at about 165'C is noteworthy. [0169] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea tromethamine salt in new crystalline forms designated as form A. 20 [01701 Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea tromethamine salt in a crystalline solid form, including a substantially pure form, which provides at least one of: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 43; and 25 (ii) a DSC scan substantially in accordance with FIG. 47; herein designated as form A. [01711 In another embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea tromethamine salt in a crystalline solid form, including a substantially pure form, which provides a DSC endotherm onset at about 165 0 C; herein designated as form A. 34 WO 2008/137809 PCT/US2008/062584 Hemi Ammonium Salt Form A and B [01721 Figures 53 and 48 respectively show the DSC trace and the X-ray powder pattern for another crystalline solid form A. In the DSC trace, an endotherm onset at about 146'C is 5 noteworthy. [01731 Figures 58 and 54 respectively show the DSC trace and the X-ray powder pattern for another crystalline solid form B. In the DSC trace, an exotherm onset at about 183 C is noteworthy. [0174] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 10 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea hemi ammonium salt in new crystalline forms designated as form A and form B. [0175] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt in a crystalline solid form, including a substantially pure form, which 15 provides at least one of: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 48; and (ii) a DSC scan substantially in accordance with FIG. 53; herein designated as form A. [0176] In another embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 20 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi ammonium salt in a crystalline solid form, including a substantially pure form, which provides a DSC maximum endotherm at about 146 'C; herein designated as form A. [0177] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea hemi 25 ammonium salt in a crystalline solid form, including a substantially pure form, which provides at least one of: (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 54; and (ii) a DSC scan substantially in accordance with FIG. 58; herein designated as form B. 35 WO 2008/137809 PCT/US2008/062584 L-lysine Salt Form A [0178] Figure 59 shows the X-ray powder pattern for an amorphous form. [0179] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo- 1, 4 -dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 5 sulfonylurea L-lysine salt in an amorphous form. [0180] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea L-lysine salt in an amorphous form, including a substantially pure form, which provides an X-ray powder diffraction pattern substantially in accordance with FIG. 59; herein designated as 10 amorphous. Magnesium Salt Form A [01811 Figure 61 shows the X-ray powder pattern for an amorphous form. [0182] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 15 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea magnesium salt in new crystalline forms designated as form A. [0183] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea magnesium salt in a crystalline solid form, including a substantially pure form, which 20 provides an X-ray powder diffraction pattern substantially in accordance with FIG. 61; herein designated as form A. L-arginine Salt amorphous form [01841 Figure 64 shows the X-ray powder pattern for the amorphous forms. 25 [0185] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea L-arginine salt in an amorphous form. [0186] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea

L

36 WO 2008/137809 PCT/US2008/062584 arginine in amorphous form, including a substantially pure form, which provides an X-ray powder diffraction pattern substantially in accordance with FIG. 64; herein designated as amorphous. 5 N-ethylglucamine Salt amorphous form [01871 Figure 66 shows the X-ray powder pattern for an amorphous form. [0188] Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea N-ethylglucamine salt in an amorphous form. 10 [01891 Thus in one embodiment, the invention provides [ 4 -(6-fluoro-7-methylamino-2,4 dioxo- 1,4-dihydro-2H-quinazolin-3 -yl)-phenyl] -5 -chloro-thiophen-2-yl-sulfonylurea

N

ethylglucamine in amorphous form, including a substantially pure form, which provides an X-ray powder diffraction pattern substantially in accordance with FIG. 66; herein designated as amorphous. 15 N-methylglucamine Salt amorphous form [0190] Figure 67 shows the X-ray powder pattern for an amorphous form. [01911 Thus in one embodiment, the present invention provides [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 20 sulfonylurea N-methylglucamine salt in an amorphous form. [0192] Thus in one embodiment, the invention provides [4-(6-fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea

N

methylglucamine in amorphous form, including a substantially pure form, which provides an X-ray powder diffraction pattern substantially in accordance with FIG. 67; herein designated 25 as amorphous. [0193] Crystalline form A of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt is a 2.5 hydrate which is stable between 20-90%RH at 25 0 C but which dehydrates between 20 and 0% RH at 25 0 C. Form A of the potassium salt has been found to be equally stable as the amorphous 37 WO 2008/137809 PCT/US2008/062584 form of the sodium salt. No change in the chemical purity of either salt form was observed after one week when in accelerated stability tests at high temperature (40 'C) and high relative humidity (75% RH). An advantage of the potassium crystalline form A is that it is less hygroscopic than the amorphous form of the sodium salt which picks up > 15% w/w 5 water at 40% RH. Both K salts? form A and B are stable to what?. Form B of the potassium salt is hemihydrate and non-hygroscopic. Form B of the potassium salt retains a better physical appearance and handling properties over a longer period of time. An improvement in the physical appearance of a dosage form of a drug enhances both physician and patient acceptance and increases the likelihood of success of the treatment. 10 [01941 Further embodiments of the invention include mixtures of the different crystalline solid forms, and the amorphous form, of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea and its salts. Such mixtures include compositions comprising at least one solid form or at least two solid forms selected from form A, form B, form C, form D and the amorphous form. Any of the 15 analytical techniques described herein may be used to detect the presence of the solid forms in such compositions. Detection may be done qualitatitvely, quantitatively, or semi quantitatively as those terms as used and understood by those of skill in the solid-state analytical arts. [0195] For these analyses, use of standard analytical techniques involving reference 20 standards may be used. Further, such methods may include use of techniques such as least squares in conjunction with a spectroscopic analytical technique. These techniques may also be used in pharmaceutical compositions of the invention. V. Preparation of Crystalline solid and Amorphous forms of the Invention 25 [0196] Furthermore, the present invention is directed to processes for the preparation of crystalline solid and amorphous forms of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium and sodium salts. [0197] Crystalline solid and amorphous forms of the compounds of the invention may be 30 prepared by various methods as outlined below. Other well-known crystallization procedures as well as modification of the procedures outline above may be utilized. 38 WO 2008/137809 PCT/US2008/062584 [01981 In another embodiment of the present invention there is provided [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt in a crystalline solid form A, which is obtained by at least one of: (i) crystallizing [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl) 5 phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt from at least one solvent selected from the group consisting of ethanol, methanol, and combinations thereof and drying such that the crystal contained some solvent; (ii) recrystallisation by heating [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt in at least one 10 solvent selected from the group consisting of ethanol, methanol, and combinations thereof; crystallizing at a temperature of from about 50 C to -10 C and drying until the crystals contained at least about 0.05% solvent. (iii) heating [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] 5-chloro-thiophen-2-yl-sulfonylurea with sodium hydroxide or sodium ethoxide in 15 tetrahydrofuran; crystallizing at a temperature of from about 50 C to 25'C and drying until the crystals contained at least about 0.05% solvent. [01991 In another embodiment of the present invention there is provided [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt in a crystalline solid form B, which is obtained by at least one of: 20 (i) heating [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] 5-chloro-thiophen-2-yl-sulfonylurea potassium salt in a solvent combination of ethanol and water; crystallizing at a temperature of from about 50 C to -10 C and drying until the crystals contain less than 0.05% organic solvent; (ii) crystallizing [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl) 25 phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt from a solvent combination of ethanol and water and drying such that the crystal contained less than 0.05% organic solvent; and (iii) heating [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] 5-chloro-thiophen-2-yl-sulfonylurea in potassium hydroxide or potassium ethoxide in 30 isopropanol or a solvent combination of acetonitrile and water; crystallizing at a temperature 39 WO 2008/137809 PCT/US2008/062584 of from about 50 C to 4 0 C and drying until the crystals contain less than 0.05% organic solvent. [0200] In another embodiment of the present invention there is provided [4-(6-fluoro-7 methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl 5 sulfonylurea potassium salt in a crystalline solid form C, which is obtained by at least one of: (i) [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea was treated with 1.15 equivalent of potassium ethoxide in water; and heated for 50'C for 2 hours followed by cooling to 4' C and dried. [0201] In another embodiment of the present invention there is provided an amorphous 10 form of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5 chloro-thiophen-2-yl-sulfonylurea potassium salt by triturating in isopropanol and drying. [0202] In another embodiment of the present invention there is provided a amorphous form of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea sodium salt which is obtained by at least one of: (i) heating [4-(6 15 fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea sodium salt in at least one solvent selected from the group consisting of isopropanol, acetonitrile, ethanol and combinations thereof; and crystallizing at a temperature of from about 50 C to -10 0 C; (ii) crystallizing [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl) 20 phenyl]-5-chloro-thiophen-2-yl-sulfonylurea sodium salt from at least one solvent selected from the group consisting of isopropanol, acetonitrile, ethanol and combinations thereof; and (iii) heating [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl] 5-chloro-thiophen-2-yl-sulfonylurea with sodium hydroxide in tetrahydrofuran or isopropanol at 50 0 C followed by cooling to 25'C, filtered and dried to give sodium salt Form A. 25 [0203] Furthermore, the present invention is directed to the above described processes for the preparation of crystalline solid and amorphous forms of [4-(6-fluoro-7-methylamino-2,4 dioxo-1, 4 -dihydro- 2 H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium and sodium salts. [02041 [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5 30 chloro-thiophen-2-yl-sulfonylurea in a crystalline solid or amorphous form may be prepared 40 WO 2008/137809 PCT/US2008/062584 by various methods as further described below in the Examples. The examples illustrate, but do not limit the scope of the present invention. [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4 dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea in crystalline solid or amorphous forms may be isolated using typical isolation and purification techniques 5 known in the art, including, for example, chromatographic, and other procedures as well as modification of the procedures outlined above. [02051 In formulating [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3 yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt to prepare immediate release beads, i.e., using wet granulation followed by extrusion, spherinization and drying, the bead 10 dissolution was slow and incomplete. The XRPD pattern of the beads after compensating for the background signal was not consistent with form B of [ 4

-(

6 -fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt, the starting API form or the known form of free acid. [0206] A grinding experiment using a mortar and pestle to mimic wet granulation 15 conditions was performed. Form B of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro 2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt was ground with either 35% or 90% of water for approximately 10min and then dried in oven at 40 C overnight. The XRPD result of the sample ground with 90% of water gave a completely different XRPD pattern which was consistent with the form of the API in the beads. The 20 sample ground with 35% of water gave a similar XRPD pattern to form B. The samples were also run by TGA and DSC. Grinding form B with 75% of water for 10-20min. and analysis gave XRPD data which indicated that the API has converted to an amorphous form. The same sample was analyzed for XRPD again after 1 month storage at ambient room temperature. Based on XRPD results, the material had converted to form C. This result 25 suggests that the conversion of form B to form C probably proceeds via an amorphous phase. [0207] It was observed that after grinding the API with the diluent of Tox formulation, i.e., 0.5% methyleellulose and 0. 1M phosphate sodium buffer at pH7.4, the drug particles became very dense and quickly precipitated during transfer and dosing. The procedure was repeated and it was also found that the suspended particles quickly coalesced and formed clumps 30 which became difficult to redisperse. Work was carried out to identify a vehicle and preparation procedure that does not cause coalescence and solid state conversion. It was found that by removing 0.5% methylcellulose from the formulation, the irreversible 41 WO 2008/137809 PCT/US2008/062584 coalescence problem can be solved. In addition, if only dry grinding is used to reduce the particle size of the API first and subsequently, the API is quickly dispersed into aqueous 0.1M phosphate buffer without applying significant mechanical stress, the solid form of the API can be maintained in form B for at least 6 hours. 5 [0208] A second lot of form C was prepared by repetitive grinding with more than 90% w/w water followed by drying in a 40'C oven for at least 2 hours. During different stages of the preparation, the solid state of the API was followed by DSC and TGA. [02091 Other methods of preparing amorphous and crystalline forms of salts of the invention are provided in the Examples. 10 VI. Pharmaceutical Compositions [02101 A salt of formula (I) according to the invention may be formulated into pharmaceutical compositions. Accordingly, the invention also provides a pharmaceutical composition for preventing or treating thrombosis in a mammal, particularly those 15 pathological conditions involving platelet aggregation, containing a therapeutically effective amount of a salt of formula (I) or a pharmaceutically acceptable salt thereof, each as described above, and a pharmaceutically acceptable carrier or agent. Preferably, a pharmaceutical composition of the invention contains a salt of formula (I), or a form thereof, in an amount effective to inhibit platelet aggregation, more preferably, ADP-dependent 20 aggregation, in a mammal, in particular, a human. Pharmaceutically acceptable carriers or agents include those known in the art and are described below. [0211] Pharmaceutical compositions of the invention may be prepared by mixing the salt of formula (I) with a physiologically acceptable carrier or agent. Pharmaceutical compositions of the invention may further include excipients, stabilizers, diluents and the like and may be 25 provided in sustained release or timed release formulations. Acceptable carriers, agents, excipients, stablilizers, diluents and the like for therapeutic use are well known in the pharmaceutical field, and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co., ed. A. R. Gennaro (1985). Such materials are non-toxic to the recipients at the dosages and concentrations employed, and include buffers such as 30 phosphate, citrate, acetate and other organic acid salts, antioxidants such as ascorbic acid, low molecular weight (less than about ten residues) peptides such as polyarginine, proteins, such 42 WO 2008/137809 PCT/US2008/062584 as serum albumin, gelatin, or immunoglobulins, hydrophilic polymers such as polyvinylpyrrolidinone, amino acids such as glycine, glutamic acid, aspartic acid, or arginine, monosaccharides, di-saccharides, and other carbohydrates including cellulose or its derivatives, glucose, mannose or dextrins, chelating agents such as EDTA, sugar alcohols 5 such as mannitol or sorbitol, counterions such as sodium and/or non-ionic surfactants such as TWEEN, or polyethyleneglycol. [0212] Further embodiments of the invention include pharmaceutical compositions of [4 (6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea, its salts and forms, including in therapeutically effective amounts 10 crystalline and amorphous forms of the salts disclosed herein. Said amounts of the at least one of said forms may or may not be in therapeutically effective amounts. Such pharmaceutical compositions may be in the form of a solid oral composition such as a tablet or a capsule or as a dry powder for inhalation. [0213] Wet granulation is an important method to prepare solid oral pharmaceutical dosage 15 forms. Form C of the potassium salt is a unique form that is generated during a wet granulation process. The presence of form C has hindered dissolution of spheronized beads which contain it until the beads were physically crushed. This hindered dissolution may be due to a specific interaction between form C and excipients in this particular formulation. Improved or at least equivalent dissolution behavior may be realized with different excipient 20 compositions. Pharmaceutically acceptable carriers [0214] Diagnostic applications of the salts of this invention will typically utilize formulations such as solutions or suspensions. 25 [0215] In the management of thrombotic disorders the salts of this invention may be utilized in compositions such as tablets, capsules, lozenges or elixirs for oral administration, suppositories, sterile solutions or suspensions or injectable administration, and the like, or incorporated into shaped articles. Subjects in need of treatment (typically mammalian subjects) can be administered appropriate dosages of the compounds of this invention that 30 will provide optimal efficacy. The dose and method of administration will vary from subject to subject and be dependent upon such factors as the type of mammal being treated, its sex, 43 WO 2008/137809 PCT/US2008/062584 weight, diet, concurrent medication, overall clinical condition, the particular salts employed, the specific use for which these salts are employed, and other factors which those skilled in the medical arts will recognize. [0216] Capsules useful in the present invention can be prepared using conventional and 5 known encapsulation techniques, such as that described in Stroud et al., U.S. Patent No. 5,735,105. The capsule is typically a hollow shell of generally cylindrical shape having a diameter and length sufficient so that the pharmaceutical solution compositions containing the appropriate dose of the active agent fits inside the capsule. The exterior of the capsules can include plasticizer, water, gelatin, modified starches, gums, carrageenans, and mixtures 10 thereof. Those skilled in the art will appreciate what compositions are suitable. [0217] In addition to the active agent, tablets useful in the present invention can comprise fillers, binders, compression agents, lubricants, disintegrants, colorants, water, talc and other elements recognized by one of skill in the art. The tablets can be homogeneous with a single layer at the core, or have multiple layers in order to realize preferred release profiles. In some 15 instances, the tablets of the instant invention may be coated, such as with an enteric coating. One of skill in the art will appreciate that other excipients are useful in the tablets of the present invention. [02181 Lozenges useful in the present invention include an appropriate amount of the active agent as well as any fillers, binders, disintegrants, solvents, solubilizing agents, sweeteners, 20 coloring agents and any other ingredients that one of skill in the art would appreciate is necessary. Lozenges of the present invention are designed to dissolve and release the active agent on contact with the mouth of the patient. One of skill in the art will appreciate that other delivery methods are useful in the present invention. [0219] Formulations of the salts of this invention are prepared for storage or administration 25 by mixing the salt having a desired degree of purity with physiologically acceptable carriers, excipients, stabilizers etc., and may be provided in sustained release or timed release formulations. Acceptable carriers or diluents for therapeutic use are well known in the pharmaceutical field, and are described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co., (A.R. Gennaro Ed. 1985). Such materials are non-toxic to 30 the recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate, acetate and other organic acid salts, antioxidants such as ascorbic acid, low molecular weight (less than about ten residues) peptides such as polyarginine, proteins, such 44 WO 2008/137809 PCT/US2008/062584 as serum albumin, gelatin, or immunoglobulins, hydrophilic polymers such as polyvinylpyrrolidinone, amino acids such as glycine, glutamic acid, aspartic acid, or arginine, monosaccharides, disaccharides, and other carbohydrates including cellulose or its derivatives, glucose, mannose or dextrins, chelating agents such as EDTA, sugar alcohols 5 such as mannitol or sorbitol, counterions such as sodium, and/or non-ionic surfactants such as Tween, Pluronics or polyethyleneglycol. [0220] Dosage formulations of the salts of this invention to be used for therapeutic administration must be sterile. Sterility is readily accomplished by filtration through sterile membranes such as 0.2 micron membranes, or by other conventional methods. Formulations 10 typically will be stored in lyophilized form or as an aqueous solution. The pH of the preparations of this invention typically will be between 3 and 11, more preferably from 5 to 9 and most preferably from 7 to 8. It will be understood that use of certain of the foregoing excipients, carriers, or stabilizers will result in the formation of cyclic polypeptide salts. While the preferred route of administration is by injection, other methods of administration 15 are also anticipated such as intravenously (bolus and/or infusion), subcutaneously, intramuscularly, colonically, rectally, nasally or intraperitoneally, employing a variety of dosage forms such as suppositories, implanted pellets or small cylinders, aerosols, oral dosage formulations (such as tablets, capsules and lozenges) and topical formulations such as ointments, drops and dermal patches. The sterile of this invention are desirably incorporated 20 into shaped articles such as implants which may employ inert materials such as biodegradable polymers or synthetic silicones, for example, Silastic, silicone rubber or other polymers commercially available. [0221] The salts of the invention may also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar 25 vesicles. Liposomes can be formed from a variety of lipids, such as cholesterol, stearylamine or phosphatidylcholines. [0222] The salts of this invention may also be delivered by the use of antibodies, antibody fragments, growth factors, hormones, or other targeting moieties, to which the salt molecules are coupled. The salts of this invention may also be coupled with suitable polymers as 30 targetable drug carriers. Such polymers can include polyvinylpyrrolidinone, pyran copolymer, polyhydroxy-propyl-methacrylamide-phenol, polyhydroxyethyl-aspartamide phenol, or polyethyleneoxide-polylysine substituted with palmitoyl residues. Furthermore, 45 WO 2008/137809 PCT/US2008/062584 salts of the invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example polylactic acid, polyglycolic acid, copolymers of polylactic and polyglycolic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross 5 linked or amphipathic block copolymers of hydrogels. Polymers and semipermeable polymer matrices may be formed into shaped articles, such as valves, stents, tubing, prostheses and the like. Dosing 10 [0223] Typically, about 0.5 to 500 mg of a salt or mixture of salts of this invention is compounded with a physiologically acceptable vehicle, carrier, excipient, binder, preservative, stabilizer, dye, flavor etc., as called for by accepted pharmaceutical practice. The amount of active ingredient in these compositions is such that a suitable dosage in the range indicated is obtained. 15 [0224] It is contemplated that a typical dosage will range from about 0.001 mg/kg to about 1000 mg/kg, preferably from about 0.01 mg/kg to about 100 mg/kg, and more preferably from about 0.10 mg/kg to about 20 mg/kg. The compounds of this invention may be administered once or several times daily and other dosage regimens may also be useful. 20 VII. Methods of Treatment/Administration A. Preventing and treating disease conditions characterized by undesired thrombosis [0225] Methods for preventing or treating thrombosis in a mammal embraced by the invention administering a therapeutically effective amount of a salt of formula (I) alone or as 25 part of a pharmaceutical composition of the invention as described above to a mammal, in particular, a human. Compounds of formula (I) and pharmaceutical compositions of the invention containing a salt of formula (I) of the invention are suitable for use alone or as part of a multi-component treatment regimen for the prevention or treatment of cardiovascular diseases, particularly those related to thrombosis. For example, a compound or 30 pharmaceutical composition of the invention may be used as a drug or therapeutic agent for 46 WO 2008/137809 PCT/US2008/062584 any thrombosis, particularly a platelet-dependent thrombotic indication, including, but not limited to, acute myocardial infarction, unstable angina, chronic stable angina, transient ischemic attacks, strokes, peripheral vascular disease, preeclampsia/eclampsia, deep venous thrombosis, embolism, disseminated intravascular coagulation and thrombotic cytopenic 5 purpura, thrombotic and restenotic complications following invasive procedures, e.g., angioplasty, carotid endarterectomy, post CABG (coronary artery bypass graft) surgery, vascular graft surgery, stent placements and insertion of endovascular devices and protheses, and hypercoagulable states related to genetic predisposition or cancers. In other groups of embodiments, the indication is selected from the group consisting of percutaneous coronary 10 intervention (PCI) including angioplasty and/or stent, acute myocardial infarction (AMI), unstable angina (USA), coronary artery disease (CAD), transient ischemic attacks (TIA), stroke, peripheral vascular disease (PVD), Surgeries-coronary bypass, carotid endarectomy [0226] Compounds and pharmaceutical compositions of the invention may also be used as part of a multi-component treatment regimen in combination with other therapeutic or 15 diagnostic agents in the prevention or treatment of thrombosis in a mammal. In certain preferred embodiments, compounds or pharmaceutical compositions of the invention may be co-administered along with other compounds typically prescribed for these conditions according to generally accepted medical practice such as anticoagulant agents, thrombolytic agents, or other antithrombotics, including platelet aggregation inhibitors, tissue plasminogen 20 activators, urokinase, prourokinase, streptokinase, heparin, aspirin, or warfarin or anti inflammatories (non-steriodal anti-inflammatories, cyclooxygenase II inhibitors). Co administration may also allow for application of reduced doses of both the anti-platelet and the thrombolytic agents and therefore minimize potential hemorrhagic side-effects. Compounds and pharmaceutical compositions of the invention may also act in a synergistic 25 fashion to prevent re-occlusion following a successful thrombolytic therapy and/or reduce the time to reperfusion. [02271 The compounds and pharmaceutical compositions of the invention may be utilized in vivo, ordinarily in mammals such as primates, (e.g., humans), sheep, horses, cattle, pigs, dogs, cats, rats and mice, or in vitro. The biological properties, as defined above, of a 30 compound or a pharmaceutical composition of the invention can be readily characterized by methods that are well known in the art such as, for example, by in vivo studies to evaluate antithrombotic efficacy, and effects on hemostasis and hematological parameters. 47 WO 2008/137809 PCT/US2008/062584 [0228] Compounds and pharmaceutical compositions of the invention may be in the form of solutions or suspensions. In the management of thrombotic disorders the compounds or pharmaceutical compositions of the invention may also be in such forms as, for example, tablets, capsules or elixirs for oral administration, suppositories, sterile solutions or 5 suspensions or injectable administration, and the like, or incorporated into shaped articles. Subjects (typically mammalian) in need of treatment using the compounds or pharmaceutical compositions of the invention may be administered dosages that will provide optimal efficacy. The dose and method of administration will vary from subject to subject and be dependent upon such factors as the type of mammal being treated, its sex, weight, diet, 10 concurrent medication, overall clinical condition, the particular salt of formula (I) employed, the specific use for which the compound or pharmaceutical composition is employed, and other factors which those skilled in the medical arts will recognize. B. Therapeutically effective amount 15 [02291 Dosage formulations of compounds of formula (I), or pharmaceutical compositions contain a compound of the invention, to be used for therapeutic administration must be sterile. Sterility is readily accomplished by filtration through sterile membranes such as 0.2 micron membranes, or by other conventional methods. Formulations typically will be stored in a solid form, preferably in a lyophilized form. While the preferred route of administration 20 is orally, the dosage formulations of compounds of formula (I) or pharmaceutical compositions of the invention may also be administered by injection, intravenously (bolus and/or infusion), subcutaneously, intramuscularly, colonically, rectally, nasally, transdermally or intraperitoneally. A variety of dosage forms may be employed as well including, but not limited to, suppositories, implanted pellets or small cylinders, aerosols, oral 25 dosage formulations and topical formulations such as ointments, drops and dermal patches. The compounds of formula (I) and pharmaceutical compositions of the invention may also be incorporated into shapes and articles such as implants which may employ inert materials such biodegradable polymers or synthetic silicones as, for example, SILASTIC, silicone rubber or other polymers commercially available. The compounds and pharmaceutical compositions of 30 the invention may also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes 48 WO 2008/137809 PCT/US2008/062584 can be formed from a variety of lipids, such as cholesterol, stearylamine or phosphatidyicholines. [0230] Therapeutically effective dosages may be determined by either in vitro or in vivo methods. For each particular compound or pharmaceutical composition of the present 5 invention, individual determinations may be made to determine the optimal dosage required. The range of therapeutically effective dosages will be influenced by the route of administration, the therapeutic objectives and the condition of the patient. For injection by hypodermic needle, it may be assumed the dosage is delivered into the body's fluids. For other routes of administration, the absorption efficiency must be individually determined for 10 each compound by methods well known in pharmacology. Accordingly, it may be necessary for the therapist to titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect. The determination of effective dosage levels, that is, the dosage levels necessary to achieve the desired result, will be readily determined by one skilled in the art. Typically, applications of compound are commenced at lower dosage 15 levels, with dosage levels being increased until the desired effect is achieved. [0231] The determination of effective dosage levels, that is, the dosage levels necessary to achieve the desired result, i.e., platelet ADP receptor inhibition, will be readily determined by one skilled in the art. Typically, applications of a compound or pharmaceutical composition of the invention are commenced at lower dosage levels, with dosage levels being increased 20 until the desired effect is achieved. The compounds and compositions of the invention may be administered orally in an effective amount within the dosage range of about 0.0 1 to 1000 mg/kg in a regimen of single or several divided daily doses. If a pharmaceutically acceptable carrier is used in a pharmaceutical composition of the invention, typically, about 5 to 500 mg of a salt of formula (I) is compounded with a pharmaceutically acceptable carrier as called for 25 by accepted pharmaceutical practice including, but not limited to, a physiologically acceptable vehicle, carrier, excipient, binder, preservative, stabilizer, dye, flavor, etc. The amount of active ingredient in these compositions is such that a suitable dosage in the range indicated is obtained. 49 WO 2008/137809 PCT/US2008/062584 C. Administration [0232] Therapeutic compound liquid formulations generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having a stopper pierceable by hypodermic injection needle. 5 [0233] Typical adjuvants which may be incorporated into tablets, capsules, lozenges and the like are binders such as acacia, corn starch or gelatin, and excipients such as microcrystalline cellulose, disintegrating agents like corn starch or alginic acid, lubricants such as magnesium stearate, sweetening agents such as sucrose or lactose, or flavoring agents. When a dosage form is a capsule, in addition to the above materials it may also 10 contain liquid carriers such as water, saline, or a fatty oil. Other materials of various types may be used as coatings or as modifiers of the physical form of the dosage unit. Sterile compositions for injection can be formulated according to conventional pharmaceutical practice. For example, dissolution or suspension of the active compound in a vehicle such as an oil or a synthetic fatty vehicle like ethyl oleate, or into a liposome may be desired. 15 Buffers, preservatives, antioxidants and the like can be incorporated according to accepted pharmaceutical practice. D. Combination therapies [0234] The compounds of the present invention may also be used in combination with other 20 therapeutic or diagnostic agents. In certain preferred embodiments, the compounds of this invention may be co-administered along with other compounds typically prescribed for these conditions according to generally accepted medical practice such as anticoagulant agents, thrombolytic agents, or other antithrombotics, including platelet aggregation inhibitors, tissue plasminogen activators, urokinase, prourokinase, streptokinase, heparin, aspirin, or warfarin. 25 The compounds of the present invention may act in a synergistic fashion to prevent re occlusion following a successful thrombolytic therapy and/or reduce the time to reperfusion. These compounds may also allow for reduced doses of the thrombolytic agents to be used and therefore minimize potential hemorrhagic side-effects. The compounds of this invention can be utilized in vivo, ordinarily in mammals such as primates, (e.g. humans), sheep, horses, 30 cattle, pigs, dogs, cats, rats and mice, or in vitro. 50 WO 2008/137809 PCT/US2008/062584 [0235] It should be understood that the foregoing discussion, embodiments and examples merely present a detailed description of certain preferred embodiments. It will be apparent to those of ordinary skill in the art that various modifications and equivalents can be made without departing from the spirit and scope of the invention. All the patents, journal articles 5 and other documents discussed or cited above are herein incorporated by reference. [0236] The following preparations and examples are given to enable those skilled in the art to more clearly understand and to practice the present invention. They should not be considered as limiting the scope of the invention, but merely as being illustrative and representative thereof. 10 VIII. Examples [0237] Unless stated otherwise, the abbreviations used throughout the specification have the following meanings: A Angstrom A% total percent area aq. aqueous AcN, ACN acetonitrile, methyl cyanide n-BuOAc n-butyl acetate s-BuOAc s-butyl acetate ca. approximately cm centimeter ClPh chlorophenol d doublet DCE dichloroethane DCM dichloromethane, methylene chloride DIPE di-isopropylether DMA dimethyl acetamide DMF dimethyl formamide DS drug substance DSC differential scanning calorimetry EDTA ethylenediaminetetraacetic acid Et 2 O di-ethyl ether EtOAc ethyl acetate EtOH ethanol, ethyl alcohol eq. equivalent 51 WO 2008/137809 PCT/US2008/062584 f.a. free acid f.b. free base g gram

H

2 0 water - distilled or HPLC grade HPLC high performance liquid chromatography hr hour Hz Hertz IR infrared IPA iso-propy alcohol, propan-2-ol iPrOAc iso-propyl acetate iPrOH iso-propy alcohol, propan-2-ol J coupling constant kg kilogram kV kilovolts L liter LOD limit of detection LNB Laboratory Note Book MeCN methyl cyanide, acetonitrile MEK methyl ethyl ketone, butanone M molar m multiplet mA milliampere Me methyl MeOH methanol, methyl alcohol MIBK methyl isobutyl ketone, 2,2-dimethyl butan-3-one mg milligram min. minute mL milliliter mm millimeter MTBE tertiary butyl methyl ether nBuOH n-butanol, butan- 1 -ol N normal nM nanomolar NMP n-methyl pyrrolidone NMR nuclear magnetic resonance nPrOH n-propanol, propan- 1 -ol PF Project Folder PTFE polytetrafluoroethene, polytetrafluoroethylene RM reaction mixture RT room temperature s singlet 52 WO 2008/137809 PCT/US2008/062584 SM starting material tBME / TBME t-butyl methyl ether tBuOH t-butanol ( 2-methyl-propan-2-ol) TDS total dissolved solids TGA thermal gravimetric analysis THF tetrahydrofuran TMP 2,2,4-trimethylpentane, iso-octane pM micromolar General methods [02381 The starting materials and reagents used in preparing these compounds generally are either available from commercial suppliers, such as Aldrich Chemical Co., or are prepared by 5 methods known to those skilled in the art following procedures set forth in references such as Fieser and Fieser 's Reagentsfor Organic Synthesis; Wiley & Sons: New York, 1967-2004, Volumes 1-22; Rodd's Chemistry of Carbon Compounds, Elsevier Science Publishers, 1989, Volumes 1-5 and Supplementals; and Organic Reactions, Wiley & Sons: New York, 2005, Volumes 1-65. The following synthetic reaction schemes are merely illustrative of some 10 methods by which the compounds of the present invention can be synthesized, and various modifications to these synthetic reaction schemes can be made and will be suggested to one skilled in the art having referred to the disclosure contained in this Application. [0239] The starting materials and the intermediates of the synthetic reaction schemes can be isolated and purified if desired using conventional techniques, including but not limited to, 15 filtration, distillation, crystallization, chromatography, and the like. Such materials can be characterized using conventional means, including physical constants and spectral data. [0240] Unless specified to the contrary, the reactions described herein preferably are conducted under an inert atmosphere at atmospheric pressure at a reaction temperature range of from about -78 'C to about 150 'C, more preferably from about 0 'C to about 125 'C, and 20 most preferably and conveniently at about room (or ambient) temperature, e.g., about 20 'C to about 75 "C. [02411 Referring to the examples that follow, compounds of the present invention were synthesized using the methods described herein, or other methods, which are well known in the art. 53 WO 2008/137809 PCT/US2008/062584 [0242] The compounds and/or intermediates were characterized by high performance liquid chromatography (HPLC) using a Waters Alliance chromatography system with a 2695 Separation Module (Milford, Mass.). The analytical columns were C-18 SpeedROD RP-18E Columns from Merck KGaA (Darmstadt, Germany). Alternately, characterization was 5 performed using a Waters Unity (UPLC) system with Waters Acquity UPLC BEH C-18 2.1 mm x 15 mm columns. A gradient elution was used, typically starting with 5% acetonitrile/95% water and progressing to 95% acetonitrile over a period of 5 minutes for the Alliance system and 1 minute for the Acquity system. All solvents contained 0.1% trifluoroacetic acid (TFA). Compounds were detected by ultraviolet light (UV) absorption at 10 either 220 or 254 nm. HPLC solvents were from EMD Chemicals, Inc. (Gibbstown, NJ). In some instances, purity was assessed by thin layer chromatography (TLC) using glass backed silica gel plates, such as, for example, EMD Silica Gel 60 2.5 cm x 7.5 cm plates. TLC results were readily detected visually under ultraviolet light, or by employing well known iodine vapor and other various staining techniques. 15 [0243] Mass spectrometric analysis was performed on one of two Agilent 1100 series LCMS instruments with acetonitrile / water as the mobile phase. One system using TFA as the modifier and measures in positive ion mode [reported as MH+, (M+1) or (M+H)+] and the other uses either formic acid or ammonium acetate and measures in both positive [reported as MH+, (M+1) or (M+H)+] and negative [reported as M-, (M-1) or (M-H)~] ion 20 modes. [0244] Nuclear magnetic resonance (NMR) analysis was performed on some of the compounds with a Varian 400 MHz NMR (Palo Alto, Calif.). The spectral reference was either TMS or the known chemical shift of the solvent. [0245] The purity of some of the invention compounds is assessed by elemental analysis 25 (Robertson Microlit, Madison NJ.). [0246] Melting points are determined on a Laboratory Devices Mel-Temp apparatus (Holliston, Mass.). [0247] Preparative separations were carried out using either an Sql6x or an Sg100c chromatography system and prepackaged silica gel columns all purchased from Teledyne 30 Isco, (Lincoln, NE). Alternately, compounds and intermediates were purified by flash column chromatography using silica gel (230-400 mesh) packing material, or by HPLC using a C-1 8 reversed phase column. Typical solvents employed for the Isco systems and flash 54 WO 2008/137809 PCT/US2008/062584 column chromatography were dichloromethane, methanol, ethyl acetate, hexane, acetone, aqueous hydroxyamine and triethyl amine. Typical solvents employed for the reverse phase HPLC were varying concentrations of acetonitrile and water with 0.1% trifluoroacetic acid. 5 Instrumental and Methodology Details for solid forms 1. FT Infrared Spectroscopy (FTIR) [02481 Samples were studied on a Perkin-Elmer Spectrum One fitted with a Universal ATR sampling accessory and running Spectrum V5.0.1 software. The resolution was set to 4cm-1 and 16 scans were collected over the range 4000cm-1 to 400cm-1. Control and Analysis 10 software: Spectrum v 5.0.1. 2. Differential Scanning Calorimetry (DSC) [0249] DSC data (thermograms) were collected on a TA instruments Q1000 equipped with a 50 position auto-sampler or a Mettler instrument model DSC 823e, equipped with a 34 15 position auto-sampler. The energy and temperature calibration standard for both instruments was certified indium. The method used for either instrument was that the samples were heated at a rate of 1 0 0 C / min from 10 C to 250 0 C. A nitrogen purge was maintained over the sample at about 30 to 50 ml/min for the TA instrument and 50 ml/min for the Mettler instrument. 20 [02501 Between 0.5 and 3 mg of sample was used, unless otherwise stated, and all samples were sealed in an aluminum pan with a pinhole in the lid. The control software for the TA instrument was: Advantage for Q series v 2.2.0.248, Thermal Advantage Release 4.2.1. and the analysis software for the TA instrument was: Universal Analysis 2000 v 4. 1D Build 4.1.0.16. The control and the analysis software for the Mettler DSC was: STARE v. 9.01. 25 3. Thermogravimetric analysis (TGA) [0251] TGA data (thermograms) were collected on a TA Instrument Q500 TGA with a 16 position auto-sampler, or a Mettler instrument model: TGA/SDTA 851 e, with a 34 position auto-sampler. The TA instrument was temperature calibrated using certified Alumel, and the 55 WO 2008/137809 PCT/US2008/062584 Mettler instrument with certified indium. The method used for both instruments was that the samples were heated at a rate of 10 C/minute from ambient temperature to 350 C. A nitrogen purge of about 60 to 1 00ml/min was maintained over the sample. [02521 When using the TA instrument, typically 5-30 mg of each sample was loaded onto a 5 pre-tared platinum crucible and open aluminum DSC pan. The control software was: Advantage for Q series v 2.2.0.248, Thermal Advantage Release 4.2.1. and the analysis software: Universal Analysis 2000 v 4.lD Build 4.1.0.16. When using the Mettler instrument typically 5-10 mg sample was placed in an open aluminum pan. The software for this instrument (instrument control and data analysis) was: STARE v. 9.01. 10 4. XRPD (X-Ray Powder Diffraction) Bruker AXS C2 GADDS Diffractometer [02531 X-Ray Powder Diffraction patterns were collected on a Bruker AXS C2 GADDS diffractometer using Cu Koa radiation (40kV, 40mA), automated XYZ stage, laser video 15 microscope for auto-sample positioning and a HiStar 2-dimensional area detector. X-ray optics consists of a single Gibel multilayer mirror coupled with a pinhole collimator of 0.3mm. [0254] The beam divergence, i.e. the effective size of the X-ray beam on the sample, was approximately 4 mm. A 0-0 continuous scan mode was employed with a sample - detector 20 distance of 20 cm which gives an effective 20 range of 3.2 - 29.7'. Typically the sample would be exposed to the X-ray beam for 120 seconds. Ambient conditions [02551 Samples run under ambient conditions were prepared as flat plate specimens using powder as received without grinding. Approximately 1-2mg of the sample was lightly 25 pressed on a glass slide or silicon wafer to obtain a flat surface. Single Crystal XRD (SCXRD) [0256] Data were collected on a Bruker AXS 1K SMART CCD diffractometer or a Bruker Nonius Kappa CCD equipped with an Oxford Cryosystems Cryostream cooling device. Structures were solved using either the SHELXS or SHELXD programs and refined with the 30 SHELXL program as part of the Bruker AXS SHELXTL suite. Unless otherwise stated, 56 WO 2008/137809 PCT/US2008/062584 hydrogen atoms attached to carbon were placed geometrically and allowed to refine with a riding isotropic displacement parameter. Hydrogen atoms attached to a heteroatom were located in a difference Fourier synthesis and were allowed to refine freely with an isotropic displacement parameter. 5 5. Gravimetric Vapor Sorption (GVS) Studies [02571 Sorption isotherms were obtained using a Hiden IGASorp moisture sorption analyser, controlled by CFRSorp software. The sample temperature was maintained at 25'C by a Huber re-circulating water bath. The humidity was controlled by mixing streams of dry 10 and wet nitrogen, with a total flow rate of 250ml.min'. The relative humidity was measured by a calibrated Vaisala RH probe (dynamic range of 0-95%RH), located near the sample. The weight change, (mass relaxation) of the sample as a function of %RH was constantly monitored by the microbalance (accuracy ±0.00 1mg). [0258] Typically 10-20mg of sample was placed in a tared mesh stainless steel basket 15 under ambient conditions. The sample was loaded and unloaded at 40%RH and 25'C (typical room conditions). [02591 A moisture sorption isotherm was performed as outlined below (2 scans giving 1 complete cycle). The standard isotherm was performed at 25'C at 10%RH intervals over a 0 90%RH range. Parameters Values Adsorption - Scan 1 40 - 90 Desorption / Adsorption - Scan 2 85 - Dry, Dry - 40 Intervals (%RH) 10 Number of Scans 2 Flow rate (ml.min') 250 Temperature ('C) 25 Stability (C.minl) 0.05 Minimum Sorption Time (hours) 1 Maximum Sorption Time (hours) 4 Mode AF2 Accuracy (%) 98 20 [0260] The software uses a least squares minimisation procedure together with a model of the mass relaxation, to predict an asymptotic value. The measured mass relaxation value 57 WO 2008/137809 PCT/US2008/062584 must be within 5% of that predicted by the software, before the next %RH value is selected. The minimum equilibration time was set to I hour and the maximum to 4 hours. [0261] The sample was recovered after completion of the isotherm and re-analysed by XRPD. 5 6. 'H NMR [02621 Spectra were collected on a Bruker 400MHz equipped with auto sampler. Samples were prepared in d 6 -DMSO, unless otherwise stated. 10 7. Purity Analysis [0263] Purity analysis was performed on an Agilent HP1100 system equipped with a diode array detector and using ChemStation software v9. Type of method Normal Phase Reverse Phase X Isocratic Gradient X Column: Betabasic C18, 5p tm, 150 x 4.6mm Column Temperature ('C): 25 Injection (ptl): 5 Detection: 325 Wavelength, Bandwidth( nm): 90 Flow Rate (ml.min-1): 0.8 Phase A: formic acid v/v in water Phase B: Acetonitrile : water 90:10 with 0.1% v/v formic acid Timetable: Time (min) % Phase A % Phase B 0 90 10 2 90 10 17 10 90 21 10 90 21.3 90 10 25 90 10 Type of method Normal Phase Reverse Phase X Isocratic Gradient X Column: Phenomenex Luna C18 (2), 150x4.6mm, 5 Vm Column Temperature (C): 25 Injection (r1): 5 Detection: 255 Wavelength, Bandwidth( nm): 90 58 WO 2008/137809 PCT/US2008/062584 Flow Rate (ml.min-1): 0.8-1.0 Phase A: 0.1% TFA v/v in water Phase B: 0.085% TFA in acetonitrile Timetable: Time (min) % Phase A % Phase B 0 95 5 25 5 95 25.2 95 5 30 95 5 potassium salt sodium salt Purity 99.4% (a/a) 99.4% (a/a) Impurities Individual peaks > 0.1% % (a/a) % (a/a) (a/a) RRT = 0.57 0.14 0.11 RRT = 1.08 0.15 0.18 Total of peaks <0.1% (a/a) 0.3 0.3 8 Polarised Light Microscopy (PLM) 5 [0264] Samples were studied on a Leica LM/DM polarised light microscope with a digital video camera for image capture. A small amount of each sample was placed on a glass slide, mounted in immersion oil and covered with a glass slip, the individual particles being separated as well as possible. The sample was viewed with appropriate magnification and partially polarised light, coupled to a ? false-colour filter, 10 9 Hot Stage Microscopy (HSM) [02651 Hot Stage Microscopy was carried out using a Leica LM/DM polarised light microscope combined with a Mettler-Toledo MTFP82HT hot-stage and a digital video camera for image capture A small amount of each sample was placed onto a glass slide with 15 individual particles separated as well as possible The sample was viewed with appropriate magnification and partially polarised light, coupled to a k false-colour filter, whilst being heated from ambient temperature typically at 10-20'C.min-i. 59 WO 2008/137809 PCT/US2008/062584 10. Water Determination by Karl Fischer [02661 The water content of each sample was measured on a Mettler Toledo DL39 Coulometer using Hydranal Coulomat AG reagent and an argon purge. Weighed solid samples were introduced into the vessel on a platinum TGA pan which was connected to a 5 subaseal to avoid water ingress. Approx 10mg of sample was used per titration and duplicate determination were made. 11. Aqueous Solubility [02671 Aqueous solubility was determined by suspending sufficient compound in 0.25ml of 10 water to give a maximum final concentration of>IOmg.ml-1 of the parent free-form of the compound. The suspension was equilibrated at 25'C for 24 hours then the pH was measured. The suspension was then filtered through a glass fibre C filter into a 96 well plate. The filtrate was then diluted by a factor of 101. Quantitation was by HPLC with reference to a standard solution of approximately 0.1 mg.ml-1. in DMSO. Different volumes of the standard, diluted 15 and undiluted sample solutions were injected. The solubility was calculated using the peak areas determined by integration of the peak found at the same retention time as the principal peak in the standard injection. If there was sufficient solid in the filter plate, the XRPD pattern was collected. Type of method: Reverse phase with gradient elution Column: Phenomenex Luna, C18 (2) 54m 50 x 4.6mm Column Temperature ('C): 25 Injection (pl): 5, 8 and 50 Detection: Wavelength, Bandwidth (nm): Flow Rate (ml.min'): 2 Phase A: 0.1% TFA in water Phase B: 0.085% TFA in acetonitrile Timetable: Time (m) % Phase A % Phase B 0.0 95 5 1.0 80 20 2.3 5 95 3.3 5 95 3.5 95 5 4.4 8 95 5 60 WO 2008/137809 PCT/US2008/062584 12. Ion Chromatography [0268] Data were collected on a Metrohm 861 Advanced Compact IC using IC Net software v2.3. Samples were prepared as 1000ppm stocks in water. Where sample solubility was low, a suitable solvent such as DMSO was used. Samples were diluted to 50ppm or 5 100ppm with an appropriate solvent prior to testing. Quantification was achieved by comparison with standard solutions of known concentration of the ion being analysed. Type of method Anion exchange Column: Metrosep A Supp 5 - 250 (4.0x250mm) Column Temperature ('C): Ambient Injection (ptl): 20 Detection: Conductivity detector Flow Rate (ml.min'): 0.7 Eluent: 3.2mM sodium carbonate, 1.0mM sodium hydrogen carbonate in water Type of method Cation exchange Column: Metrosep C 2 - 250 (4.0x250mm) Column Temperature ('C): Ambient Injection (ptl): 20 Detection: Conductivity detector Flow Rate (ml.min-'): 1.0 Eluent: 4.0mM Tartaric acid, 0.75mM Dipicolinic acid in water Example 1: Synthesis of the intermediate sulfonylurea carbamate (8) O CI Conc. NH 4 0H C1SO 3 H + P0. C102 6 C 0 0 C-rt. 0 0 C-to-rt.

H

2 0-THF (95 : 5) 0 0 A0 I \ Cl OEt I

H

2 N-S s CI EtO N-S CI o Cs 2

CO

3 , THF H 10 0 C-to-rt., 36 h 8 Step 1 - Preparation 5-chlorothiophene-2-sulfonyl chloride. 61 WO 2008/137809 PCT/US2008/062584 0Cl ClSO 3 H + PC 5 S C102Cl 0 C-to-rt 0102 S [0269] The following procedure was adapted from C. A. Hunt, et al. J. Med Chem. 1994, 37, 240-247. In a three-necked R.B. flask, equipped with a mechanical stirrer, an air condenser, a dropping funnel, and a moisture-guard tube, was placed chlorosulfonic acid (240 5 mL, 3.594 mol). Under stirring, PC1s (300 g, 1.44 mol, 0.40 equiv) was added in portions, over ca. 45 mins. During the addition, a large volume of HCl gas evolved vigorously, but the temperature of the mixture did not rise significantly (<40 'C). By the time all the PC 5 had been added, an almost clear, pale yellow solution resulted, with only a few solid pieces of PC1 5 floating in the suspension. It was stirred until gas evolution ceased (0.5 h). 10 [02701 Then the reaction vessel was cooled in ice, and 2-chloro-thiophene (66.0 mL, 0.715 mol) was added via the dropping funnel, over 1.0 h. With the addition of the very first few drops of 2-Cl-thiophene, the mixture turned dark purple, and by the time all of the thiophene had been added, a dark purple solution resulted. During the addition, HCl gas evolved continuously, at a slow rate. The reaction mixture was then stirred at room temperature 15 overnight. [02711 Then the mixture, dark-purple clear solution, was added dropwise to crushed ice (3 L), over 0.5 h. On addition to ice, the purple color disappeared instantaneously; the colorless thin emulsion was stirred mechanically at room temperature for ca. 15 h. Then the mixture was extracted with CH 2 Cl 2 (3 x 300 mL). The combined CH 2 Cl 2 -extract was washed with 20 water (lx 200 mL), saturated NaHC0 3 (Ix 250 mL), brine (1 x 100 mL), dried (Na 2

SO

4 ), and concentrated on a rotary evaporator to yield the crude product as a pale yellow glue, which showed a tendency to solidify, yielding a semi-solid mass. This was then purified by high vacuum distillation (bp 110-1 12/12mm) to yield 135.20 g (88%) of the title compound as a colorless/pale-yellow semi solid. 25 Step 2 - 5-chlorothiophene-2-sulfonamide. Conc. NH 4 0H 0 'C-to-rt. cj0 2 SI -cj 2---,_C C10S. S H 2 0-THF (95: 5) HN C (acidify with conc. HCI) 62 WO 2008/137809 PCT/US2008/062584 [02721 The following procedure was adapted from C. A. Hunt, et al. J. Med Chem. 1994, 37, 240-247. In a three-necked R. B. flask, equipped with a mechanical stirrer, conc. NH 4 0H (500 mL, 148.50 g NH 3 , 8.735 mol NH 3 , 13.07 equiv NH 3 ) was placed. The flask was cooled in ice and 5-chlorothiophene-2-sulfonyl chloride (145.0 g, 0.668 mol) was added, in portions 5 over 0.5 h (it is a low-melting solid, and it was melted by warming, which was then conveniently added via a wide-bored polyethylene pipette). The sulfonyl chloride immediately solidifies in the reaction flask. After all the sulfonyl chloride had been added, the flask containing it was rinsed with THF (25 mL), and this also was transferred to the reaction vessel. Then the heavy suspension was stirred at room temperature for ca. 20 h. At 10 the end of this time the reaction mixture was still a suspension but of a different texture. [0273] Then the mixture was cooled in ice, diluted with H 2 0 (1.5 1), and acidified with conc. HCl to pH ca. 3. The solid product was collected by filtration using a Buchner funnel, rinsed with cold water, and air-dried to afford the title compound as a white solid, 103.0 g (78%). MS (M-H): 196.0; 198.0 15 Step 3 - Ethyl 5-chlorothiophen-2-ylsulfonylcarbamate. 0 o C1 'A O Dt 0

H

2 N1 CI C O t EtO N-SC 11Cs 2

CO

3 , THF H 11 C 0 0 C-to-rt., 36 h 8 [02741 A 2-L 3-necked R.B. flask, equipped with a mechanical stirrer and a dropping funnel, was charged with sulfonamide (60.0 g, 303.79 mmol), and Cs 2

CO

3 (200g, 613.83 20 mmol, 2.02 equiv) in THF (900 mL). The clear solution was cooled in ice, and ethyl chloroformate (70.0 mL, 734.70 mmol, 2.418 equiv) was added over ca. 30 mins. The heavy suspension was then stirred at room temperature for ca. 36 h. [02751 Then the mixture was diluted with water (200 mL) to yield a clear colorless solution, which was concentrated on rotary evaporator to one-third its volume. This was then 25 diluted with EtOAc (250 mL), cooled in ice, and acidified with 6N HCI to pH ca. 1. The biphasic mixture was transferred to a separatory funnel, layers were separated, and the aqueous layer was again extracted with 2 x 75 mL EtOAc. The combined organic extract was washed with water/brine (2 x 50 mL), brine (1 x 50 mL), dried over Na 2

SO

4 , and concentrated to yield the title compound as lightly colored oil. This was purified by filtration 63 WO 2008/137809 PCT/US2008/062584 through a silica-gel plug. The crude product was applied to the silica-gel plug on a sintered funnel in EtOAc, and then was eluted with EtOAc (1 liter). Concentration of the EtOAc filtrate provided the title compound 8 as a white solid, 71.28 g (87%). MS (M-H): 268.0; 270.0. 'H NMR (DMSO): 6 7.62 (d, 1H), 7.25 (d, 1H), 4.10 (q, 2H), 1.16 (t, 3H). 5 Example 2: Synthesis of [4-( 6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin- 3 -yl)-phenyll-5-chloro-thiophen-2-yl-sulfonylurea (7a) Step ] F COOCH 3 20% COC1 2 in Toluene F COOCH 3 F COOCH 3 F NH 2 rt., 19h F NCO F NHCOCI 10 la 2a 2b [0276] Aniline 1 ('H NMR (DMSO): 5 7.58 (dd, IH), 6.72 (dd, 1H), 3.77 (s, 3H); 6.0 g, 32.085 mmol) was placed in a 500 mL round bottomed flask and 20% phosgene in toluene (175 mL, 332.50 mmol, 10.36 equiv) was added. The resulting somewhat sticky suspension was then magnetically stirred overnight at room temperature resulting in a clear, colorless 15 solution. An aliquot removed, blown dry with argon, quenched with MeOH, and analyzed by RP-HPLC/MS to show no unreacted aniline 1 and clean formation of the isocyanate 2a and/or carbamoyl chloride 2b as analyzed as its methyl-carbamate. The mixture was concentrated first by rotary evaporation and then under high vacuum to yield 6.76g (99% yield) of the isocyanate 2a and/or carbamoyl chloride 2b as a free-flowing white solid. 20 Step 2 64 WO 2008/137809 PCT/US2008/062584

H

2 N / NH-Boc F COOCH 3 2a and/or 2b 0 Et 3 N, DMF F N N NH-Boc H H__ 3a 4a NH-Boc 0 DBU F F N O H 4a [02771 In a 500 mL R. B. flask was placed N-Boc-1, 4-phenylenediamine (6.22 g, 29.866 mmol, 1.20 equiv) in DMF (100 mL). Triethylamine (5.30 mL, 38.025 mmol, 1.52 equiv) was syringed in. Then the clear, dark-brown solution was treated with a solution of the 5 isocyanate 2a (5.30 g, 24.88 mmol) and/or carbamoyl chloride 2b in DMF (50 mL), dropwise, over 15 minutes. After the addition was over, a slightly turbid mixture resulted, which was stirred overnight at room-temperature. An aliquot was analyzed, after quenching with MeOH, to show no unreacted isocyanate, and clean formation of the urea, 3a, and quinazoline-1, 3-dione, 4a, in a ratio of ca. 2.5: 1. MS (M-H): 388.0. 10 [0278] DBU (3.75 mL, 25.07 mmol, ca. 1.0 equiv) was then syringed in, dropwise, over 5 minutes, resulting in a clear dark-brown solution. This was stirred at room temperature for 3.0 h resulting in a turbid mixture. HPLC analysis showed no urea 3a and clean formation of the quinazoline-1,3-dione 4a. The reaction mixture was concentrated on a rotary evaporator to yield the crude product as a solid. This was dried under high vacuum, and then triturated 15 with CH 2 Cl 2

/H

2 0 (5:1) to yield 8.40 g of 4a as an almost white solid (87% yield). 'H NMR (DMSO): 6 9.39 (s, lH), 7.68 (dd, 1H), 7.45 (d, 2H), 7.03 (m, 2H), 6.98 (dd, 1H), 1.48 (s, 9H). Step 3 65 WO 2008/137809 PCT/US2008/062584 NH-Boc

NH

2 F OZ N 4N HCl F HCl Dioxane ~-NIO H H (389) 5a 4a [0279] The N-Boc-aniline 4a (4.0g, 10.28 mmol) was placed in a round-bottomed flask and 4N HCl in dioxane (50.0 mL, 200 mmol, 19.40 equiv) was added. The heavy, negligibly solvated suspension was stirred at room temperature for 5.0 h. HPLC showed no starting 5 material and clean formation of the aniline 5a. The mixture was then concentrated on a rotary evaporator to yield the crude product. The solid thus obtained was triturated with CH 2 Cl 2 to yield 3.22g of pure 5a as an almost white solid (96% yield). MS (M-H): 290.3. 'H NMR (DMSO): 6 11.75 (s, 1H), 7.88 (dd, 1H), 7.32 (in, 4H), 7.21 (dd, lH). 10 Step 4

NH

2

DFNH

2 F0 I HCl CH 3

NH

2 F0 F N~~~0 ~DMSO-THF HC. ~ H H H 5a( 3 2 5

.

5 including HCI) 5b (300) (289 w/o HCI) [02801 The difluoro-compound, 5a (1.0g, 3.072 mmol) was placed in a screw-cap sealed tube. DMSO (20 mL) was added, followed by methylamine (2.OM in THF) (15.0 mL, 30 mmol, 9.76 equiv), resulting in a clear solution. This was then heated in an oil bath to 110 0 C 15 for 3h. HPLC showed no unreacted 5a and clean formation of 5b. The mixture was then cooled to room temperature, all the MeNH 2 and THF were evaporated, and the residue was diluted with 100 mL water to precipitate 5b. After stirring for ca. 2 h at room temperature, the white solid was collected by filtration through a Buchner funnel and rinsed with H 2 0 (100 mL), and air-dried. HPLC analysis of this solid showed it to be pure and devoid of any DBU. 20 This solid was further purified by triturating with Et 2 0, and then CH 2

C

2 as in the previous route to this aniline to give 875 mg of the title compound (95% yield). MS (M+1) 301.2. 'H 66 WO 2008/137809 PCT/US2008/062584 NMR (DMSO): 6 11.10 (s, I H), 7.36 (d, I H), 6.78 (d, 2H), 6.75 (in, 1H), 6.56 (d, 2H), 6.20 (d, 1H), 5.18 (d, 2H), 2.76 (d, 3H). Step 5 - Synthesis of l-(5-chlorothiophen-2-ylsulfonyl)-3-(4-(6-fluoro-7-(methylamino)-2,4 5 dioxo-1,2-dihydroquinazolin-3(4H)-yl)phenyl)urea (6a): CI

NH

2 Acetonitrile/reflux H H S F0 b.0 N ,

H

3 .. NN0 OA S 4 } Cl ' Na 0 00 H H'O 8 H3CN) NN o H H 6a 5a [02811 The reaction mixture comprising of the aniline (5a,16.0 g, 53.33 mmol) and ethyl sulfonyl-carbamate (8, 28.77g, 106.66 mmol, 2.0 equiv) in CH 3 CN (1300 mL) was heated to reflux for 36h. During this time, the reaction mixture remained as a heavy suspension. HPLC 10 analysis showed a clean reaction, and <1% unreacted aniline. The heavy suspension was cooled to room temperature and filtered through a Buchner funnel. The white solid product was further rinsed with CH 3 CN (3 x 40 mL). HPLC of the filtrate showed the presence of only a trace amount of the desired product, most of it being the excess carbanate. The crude product was then triturated with CH 2 Cl 2 (400 mL), and the almost white solid product (6a) 15 was collected by filtration through a Buchner funnel: Yield, 25.69g (92%). MS (M+1): 524.0; 526.0. 'H NMR (DMSO): 8 11.20 (s, 1H), 9.15 (s, 1H), 7.68 (d, 1H), 7.42 (d, 2H), 7.36 (d, 1H), 7.26 (in, 1H), 7.16 (d, 2H), 6.78 (in, 1H), 6.24 (d, 1H), 2.78 (d, 3H). 20 Example 3: [4-(6-chloro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl) phenyll-5-chloro-thiophen-2-yl-sulfonylurea (6b) [02821 The compound in Example 3 is synthesized as described for Example 2 (Step 1 -5) except starting with methyl-2-amino-5-chloro-4-fluorobenzoate which was synthesized by 25 reduction of methyl-2-nitro-5-chloro-4-fluorobenzoate with Pt(S)C . 67 WO 2008/137809 PCT/US2008/062584 Example 4: Synthesis of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyll-5-chloro-thiophen-2-yl-sulfonylurea (6a) and potassium salt (7a) O

NO

2 F Cl Oa (1.2 equiv) F Me MeCH 4

CINO

4 Oe Mo. Wt 201.56 FOeNH NO 2 Fl) NH2 p-nitrophenylchloroformate Mo .a7F2N0 0 0a Mol. Wt.: 187.14 Step 1

C

1 5

H

1 0

F

2

N

2 0 6 2 Mol. Wt.: 352.25 3a HO N a F) ~ Me Ho N O F ONe N H B o c O N H B o c

H

2 N F NHF

C

11

H

16

N

2 0 2 F N Mol. Wt.: 208.26 O N-I tert-butyl 4-aminophenylcarbamate H -x

C

2 0

H

2 1

F

2

N

3 0 5 F Mo. Wt.: 421.39 H Step 2 3b C 1 qH 1 7

F

2

N

3 0 4 Mol. Wt.: 389.35 Boc = yO 4b Ci H S Or N O NH 2 HCI O NH 2 0 0 0 (2.0 equiv) F N 'N CH3NH2 (7 equiv) F)NCHCIOS DMSO N Mol. Wt.: 269.73 F N O N N O ethyl 5-chlorothiophen-2-ylsulfonylcarbamate H Step 3 H H

C

1 4

H

1

OCIF

2

N

3 0 2

C

1 5

H

13

FN

4 0 2 DMSO, A Mol. Wt.: 325.70 Mol. Wt.: 300.29 Step 4 5b 5c CI Cl H K H H S 2N KOH(1.15 equiv) H i F N S j ACN/H- 2 , F Y S~ F~' N 00 0 0 rl N 000a NI N N) N O Step 5 H HN ~ H H

C

2 0 Hl 5 01FN 5 0 5 2 C 20

H

14

CIFKN

5 0SS 2 Mo!. WI.: 523.95 Mol. Wt.: 562.04 6a 7a 68 WO 2008/137809 PCT/US2008/062584 Step 1. 0 0 FO F OMe 0 0a NO 2 F OMe I1 !:r + DCM, 40 OCN0 F

NH

2 010 Step 1I.F 0 H'

CH

7

F

2

NO

2 Mol. Wt.: 201.56 Mol. Wt.: 187.14 p-nitrophenylchloroformate Mo V. 352.25 (1.2 equiv) 3a [02831 Methyl 2-amino-4,5-difluorobenzoate (2) (38 kg, 1.0 eq) and dichloromethane (560 kg, 8X, ACS >99.5%) were charged to a PP1-R1000 reactor (2000L GL reactor). The 5 reaction mixture was agitated for 5 mins. 4-Nitrophenylchloroformate( 49.1 kg, 1.2 equiv) was charged into PP 1 -R2000 reactor (200L) followed by dichloromethane (185 kg) and agitated the contents for 5mins. After pressurizing the 200L reactor the 4 nitrophenylchloroformate solution was transferred into the 2000L reactor containing dichloromethane solution of (2). The reaction mixture was heated to 40 ± 5 0 C (reflux) under 10 nitrogen gas purge for 3 hrs. The representative TLC analysis confirmed reaction completion (in-process TLC, no compound (2) remaining; 99:1 CHCI 3 -MeOH). The solution was cooled to 30 *C and distilled off 460 kg of dichloromethane under vacuum. The 2000L reactor was charged with 520 kg of hexanes and cooled the contents of the reactor to 0 ± 5 0 C and agitated for 4 hrs. The solid obtained was filtered through GF Nutsche filter lined with a sheet 15 of T-515 LF Typar filter and a sheet of Mel-Tuf 1149-12 filter paper. The filter cake was washed with 20 kg of hexanes and vacuum dried at 35'C until constant weight attained. The dry product was discharged (70.15 kg) with 98% yield. The product confirmed by 'H NMR and TLC analysis. 69 WO 2008/137809 PCT/US2008/062584 Step 2. Synthesis of3-(4-aminophenyl)-6,7-difluoroquinazoline-2,4(]H,3H)-dione hydrochloride, compound 5b 0 H O NHO-e NO 2

H

2 N N 0 THF, Et 3 N (0.1equiv) OMe NHBoc SM

CH

16

N

2 0 2 Step 2 F NH OMo!, Wt.* 208.26 0< tertf-butyl 4-aminophenylcarbamate O N Cj 5 Hj 0

F

2

N

2 0 6 H Mol. Wt.: 352.25 C 2 0

H

2

F

2 N305 Mot. Wt.: 421.39 3a 3b 0 ~ NHBoc 0

NH

2 ' HCl NaOMe F 4N HC F NOF N 0 MeOH F H O Dioxane H

C,

9

H,

7 2 N,0 4 C1 4

H,

0 C1F 2

N

3 0 2 Mol. Wt.: 389.35 C H FNO 4b 5b [0284] The PP1-R1000 (2000L GL reactor) reactor was charged with 3a (64.4 kg, 1.0 eq), 5 anhydrous tetrahydrofuran (557 kg) and triethylamine (2.2 kg, 0.1 equiv). The charging line of 2000L GL reactor was rinsed with tetrahydrofuran (10 kg). The contents of the reactor were agitated for 25 mins during that period complete solution was obtained. The PP1 R2000 (200L HP reactor) reactor was charged with N-Boc-p-phenylenediamine (38 kg, 1.0 equiv), tetrahydrofuran (89 kg) and agitated for 30 mins until complete solution obtained. 10 The contents of the 200L HP reactor were transferred to the 2000L GL reactor containing the compound 3a and then heated at 65 ± 5 0 C for 2 hrs. The reaction was deemed complete monitored by HPLC after confirming the disappearance of starting material 3a (in-process specification < 1%). The contents of 2000L GL reactor were cooled to 20 ± 5 C and then charged with sodium methoxide (25% solution in methanol, 41.5 kg, 1.05 equiv.) over 20 15 mins. maintaining the temperature below 30 C. The charging lines were rinsed with tetrahydrofuran (10 kg). The contents were agitated at 25 ± 5 C for 4 hrs. In-process HPLC analysis confirmed the completion of the reaction when the amount of compound 3b remaining in the reaction mixture is < 1%. To this reaction mixture added filtered process water (500 kg) and distilled under vacuum the 2000L GL reactor contents into clean 200L 20 GL receiver until 300 kg of solvent is distilled. The solids obtained were filtered using GL Nutsche filter and washed with process filtered water until the color of the solid compound 4b is white to grayish. The 2000L GL reactor is charged with wet compound 4b filter cake, dioxane (340 kg) and agitated the contents for 1 hr. The filterable solid obtained were 70 WO 2008/137809 PCT/US2008/062584 filtered through GL Nutsche filter with a sheet of T-515 LF Typar filter paper. The solid cake was blow dried for 2 hrs and then charged with dioxane (200 kg) into the 2000L GL reactor. The contents were agitated for 10 min and then charged with 4 N HCI in dioxane (914 kg) over 3 hrs and maintaining the internal temperature below 30 0 C. The charging line 5 was rinsed with additional dioxane (10 kg) and the contents of the reactor were agitated for 6 hrs at 25 ± 5 C. The completion of the reaction is monitored by HPLC (in process control compound 4b is < 1% in the reaction mixture) for the conversion of compound 4b to compound 5b. The contents of the reactor were cooled to 5 ± 5 C for 2 hr and the solid obtained was filtered through GL Nutsche filter followed by washing with dioxane (50 kg). 10 The filter cake was blow dried with 8 ± 7 psi of nitrogen for 30 mins. and purity analyzed by HPLC. The filtered solid was dried to constant weight in vacuum oven at 45 C for 48 hr. The compound 5b (65.8 kg, actual yield 110.6%) was discharged and analyzed by IH NMR and HPLC analysis. 'H NMR (DMSO): 6 11.75 (s, 1H), 7.88 (dd, 1H), 7.32 (in, 4H), 7.21 (dd, 1H). 15 Step 3. Synthesis of3-(4-aminophenyl)-6-fluoro-7-(methylamino)quinazoline-2,4(]H,3H) dione, Compound 5c 0 ' NH 2 -HCI 0 NH 2

NMCHH

3

NH

2 (7 equiv), N F K- N~O DMSO - N~~ Step 3 H H

C

14

H

10

CIF

2

N

3 0 2

C

15

H

13

FN

4 0 2 Mol. Wt.: 325.70 Mol. Wt.: 300.29 5b 5c [02851 The PP1-R2000 (200 L HP reactor) was charged with compound 5b (18 kg, 1.0 eq.) 20 and pressurized with 100 ± 5 psi of nitrogen. The nitrogen from the reactor was vented through the atmospheric vent line then the condenser valve was opened and the reactor was then charged with dimethyl sulfoxide (>99.7%, 105 kg) under a blanket of argon. The reactor contents were agitated at 22 'C (19-25 C) for 15 mins and then the maximum achievable vacuum was pulled on the 200L HP reactor after closing all the remaining valves. Using the 25 established vacuum, methylamine (33% wt % in absolute ethanol, 37.2 kg) was charged to the 200L HP reactor at a rate that maintained the internal temperature at 25 ± 5 C while keeping a nitrogen blanket on the reagent solution during the charging. After rinsing the 71 WO 2008/137809 PCT/US2008/062584 charging line with dimethyl sulfoxide (5 kg) the 200L HP reactor condenser valve was closed and the reactor contents were heated to 110 ± 5 'C. The contents of the reactor were agitated for at least 5 hrs at 110 ± 5 'C. In-process HPLC taken after 5hr 40 mins showed compound 5b content of 0. 09%, indicating completion of the reaction (in-process specification < 1 %). 5 The contents of 200L HP reactor were cooled to 25 ± 5 C. While the 200L reactor is cooling, all the valves of the PP1-R1000 reactor (2000L GL reactor) were closed and the reactor was charged with process filtered water (550 kg). The contents of the 200L HP reactor were transferred to the 2000L GL reactor over 15 minutes followed by rinsing the charging line with process filtered water (50 kg). The contents of the 2000L GL reactor were 10 agitated for 2 hrs at 5 ± 5 C. The filterable solids obtained were filtered onto PPF200 (GL nutsche filter) fitted with Mel-Tuf 1149-12 filter paper under vacuum. The wet filter cake was discharged and transferred into pre-lined vacuum trays with Dupont's fluorocarbon film (Kind 1 OOA). The special oven paper (KAVON 992) was clamped down over the vacuum trays containing the wet compound 5c and it was transferred to the vacuum oven tray dryer. 15 The oven temperature was set to 55 C and compound 5c dried to a constant weight for 12 hrs. The product 5c was discharged (12.70 kg) in 76.5% yield (expected 85-95%). HPLC shows 98.96 % purity and 'H NMR confirmed the structure for compound 5c. 'H NMR (DMSO): 8 11.10 (s, 1H), 7.36 (d, 1H), 6.78 (d, 2H), 6.75 (in, 1H), 6.56 (d, 2H), 6.20 (d, 1H), 5.18 (d, 2H), 2.76 (d, 3H). 20 Step 4. 5-Chloro-N-(4-(6-fluoro-7-(methylamino)-2,4-dioxo-1,2-dihydroquinazolin-3(4H) yl)phenylcarbamoyl)thiophene-2-sulfonamide Cl N H 2 C H HS \ H 0 N N SJ '.j N DMSO, A F 000\ N NO + -'-00 H H 0 0Step 4 N NO

C

1 5

H

1 3

FN

4 0 2

C

7 HBclNO 4

S

2 H H Mol. Wt.: 300.29 Mol. Wt.: 269.73

C

20

H

15

CIFN

5 O5S 2 5c ethyl 5-chlorothiophen-2-ylsulfonylcarbamate Mol. Wt.: 523.95 6a [02861 The PP 1 -R2000 (200L HP reactor) reactor was charged with 6 (20.7 kg, 1.0 equiv), 25 Ethyl 5-chlorothiophene-2-ylsulfonylcarbamate (37.5 kg, 2.0 equiv, >95%), dimethyl sulfoxide (>99%, 75 kg) and agitated for 15 mins. While pulling maximum achievable vacuum, the 200L HP reactor Number PPI-R2000 was heated to 65 ± 5 0 C for 15 hrs. A 72 WO 2008/137809 PCT/US2008/062584 representative sample was taken from the reactor for HPLC analysis, in-process HPLC indicated <0.9% compound 5c remaining in the reaction mixture (in-process criteria for reaction completion compound 6a < 1%). The 800L reactor number PP5-R1000 was charged with process filtered water (650 kg) and then the 200L HP contents were transferred to the 5 800 L while maintaining the internal temperature below 25 0 C. The 200L HP reactor was rinsed with dimethyl sulfoxide (15 kg) and transferred to the 800L reactor which was then agitated for 2 hrs at 5 + 5 C. The solid formed was filtered through filter PP-F2000 to a 200L GL receiver under vacuum and the filter cake was rinsed with process filtered water (60 kg). A representative sample of the wet cake was taken for HPLC analysis, if the purity of 10 compound 6a is <95% (in-process control < 95%) then dichloromethane trituration is needed). The 800L GL reactor was charged with all the wet compound 6a, dichloromethane (315 kg) and the contents were agitated for 3 hrs. The solid was filtered through GL nutsche filter lined with 1 sheet of T515 LF TYPAR filter under vacuum. The filter cake was washed with dichloromethane (50 kg) and the cake was blow dried with 8 ± 7 psi of nitrogen for 15 15 mins. The filter cake was transferred into pre-lined vacuum trays with Dupont fluorocarbon film (Kind 1 00A) and then put into the vacuum oven tray dryer set at 60 C for 12 hrs. The dried compound 6a was isolated (33.6 kg, 93% yield) with HPLC purity of 93.5% and 4.3% of sulfonamide. 'H NMR confirmed the structure for compound 6a. IH NMR (DMSO): 5 11.20 (s, 1H), 9.15 (s, 1H), 7.68 (d, 1H), 7.42 (d, 2H), 7.36 (d, lH), 20 7.26 (m, 1H), 7.16 (d, 2H), 6.78 (in, 1H), 6.24 (d, 1H), 2.78 (d, 3H). Step 5. Potassium (5-chlorothiophen-2-ylsulfonyl)(4-(6-fluoro-7-(methylamino)-2,4-dioxo 1,2-dihydroquinazolin-3(4H)-yl)phenylcarbamoyl)amide, 7a CI CI K S H H 0 H NNN N N'S 0' N 2N KOH FI Fe, N0 ACN/H 2 0 N' 0Cr NN N - _ H H Step 5 H H Hc 20

H

14

CIFKN

5 0 5 s 2

C

20

H

15

CIFN

5 0 5 s 2 Mol. Wt.: 562.04 Mol. Wt.: 523.95 6a 7a 25 [0287] The 800L GL reactor number PP5-R1 000 was charged with acetonitrile (134 kg), WvFI quality water (156 kg) and the contents were agitated for 5 mins. To this, compound 6a 73 WO 2008/137809 PCT/US2008/062584 (33.6 kg, 1.0 equiv) was added and the reaction mixture was a suspension at this point. The suspension was charged with aqueous solution (WFI water, 35 kg) of potassium hydroxide (4.14 kg, 1.15 equiv, >85%) at a rate that maintains the internal temperature below 30 0 C. The charging lines were rinsed with WFI quality water (2 kg) followed by heating the 800L 5 GL reactor contents to 50 ± 5 0 C for 1 hr. The contents were then filtered hot through a bag filter, then a seven cartridge 0.2im polish filter to clean the HDPE drums. The hot filtration system was maintained through out the filtration process so no material crashed out of the solution. The 800L GL reactor jacket was cooled to 25 ± 5 0 C before proceeding to the reactor rinse. The 800L GL reactor was rinsed with a pre-mixed solution of acetonitrile (8.5 10 kg) and WFI quality water (10 kg) through the filter system into the drums labeled as 7a hot filtration. Using the pressure vessel the 800L GL reactor was rinsed with WFI quality water (20 kg) followed by acetone (20 kg) then blown dry with nitrogen (3 2 psi). The 800GL reactor bottom valve was closed and 20 ± 10 inches Hg of vacuum was pulled. The vacuum was broken and the reactor charged with the contents of the drums labeled as 7a hot filtration. 15 The 800L GL reactor number PP5-R1000 contents was cooled to 20 ± 5 C and then, using a polish filter (PP-PF09), the reactor was charged with methanol (373 kg, >99%) maintaining the internal temperature below 30 C. The contents of the 800GL reactor number PP5-RlOOO were cooled to 15 ± 5 0 C followed by agitation of the contents for 12 hrs at this temperature. During this time the filterable solids were filtered through a clean filter apparatus (PP-F1000) 20 into clean 200L GL receiver (PPR-04) followed by pressurization of the reactor. 20 + 10 inches Hg of vacuum was pulled on the filter/receiver and the contentswere filtered. The filter cake was washed with methanol (30 kg) and blown dry with 8 ± 7 psi of nitrogen for 10 mins. The vacuum oven tray dryer temperature was set to 80 C prior to loading the wet cake of 7a. The wet filter cake was transferred into the pre-lined vacuum trays with Dupont's 25 fluorocarbon film -Kind 1 OOA and the special oven paper (Kavon Mel Tuf paper) was clamped down over the vacuum trays containing the wet product 7a. The trays were transferred to the vacuum oven tray dryer. The wet 7a was dried to a constant weight (constant weight is defined as tray reading at least 1 hr apart having the same weight within 50 g. The representative sample was analyzed for residual solvents (residual solvent 30 specifications for API) and it met the specifications. The final API was subjected to equilibration with water (5-6%) for 12 hrs with a tray of WFI quality water present, then thoroughly turned and allowed to stand for an additional 12 hrs and finally subjected to KF analysis (5.5% water content). Compound 7 potassium salt (21.80 kg, 60.6% yield) was 74 WO 2008/137809 PCT/US2008/062584 transferred to double heavy-duty poly bags and stored in secondary containment. HPLC showed a purity of 99.7% for 7a and 'H NMR confirmed the structure for 7a. 'H NMR (DMSO): 6 11.14 (s, 1H), 8.60 (s, lH), 7.48 (m, 2H), 7.35 (d, 1H), 7.22 (d, 1H), 6.95 (in, 3H), 6.75 (in, 1H), 6.22 (d, 1H), 2.78 (d, 3H). 5 Example 5: Pharmacological Assays [02881 The pharmacological activity of each of the compounds according to the invention is determined by the following in vitro assays: I. Inhibition of ADP-Mediated Platelet Aggregation In Vitro 10 1. [02891 The effect of testing the compound according to the invention on ADP-induced human platelet aggregation was assessed in a 96-well microtiter assay (see generally the procedures in Jantzen, H. M. et al. (1999) Thromb. Hemost. 81:111-117) or standard cuvette light transmittance aggregometry using either human platelet-rich plasma (PRP) or human 15 washed platelets. [0290] For preparation of human platelet-rich plasma for aggregation assays, human venous blood was collected from healthy, drug-free volunteers into 0.38 % sodium citrate (0.013 M, pH 7.0 final). Platelet-rich plasma (PRP) is prepared by centrifugation of whole blood at 160 x g for 20 minutes at room temperature. The PRP layer is removed, transferred 20 to a new tube, and the platelet count is adjusted, if necessary, to achieve a platelet concentration of~3 x 108 platelets/ml using platelet-poor plasma (PPP). PPP is prepared by centrifugation of the remaining blood sample (after removal of PRP) for 20 minutes at 800 x g. This preparation of PRP can subsequently be used for aggregation assays in either a 96 well plate or standard cuvette aggregometry. 25 [0291] For preparation of washed platelets, human venous blood is collected from healthy, drug-free volunteers into ACD (85 mM sodium citrate, 111 mM glucose, 71.4 mM citric acid) containing PGI 2 (1.25 ml ACD containing 0.2 ptM PGI2 final; PGI 2 was from Sigma, St. Louis, Mo.). Platelet-rich plasma (PRP) is prepared by centrifugation at 160 x g for 20 minutes at room temperature. Washed platelets are prepared by centrifuging PRP for 10 30 minutes at 730 x g and re-suspending the platelet pellet in CGS (13 mM sodium citrate, 30 mM glucose, 120 mM NaCl; 2 ml CGS/10 ml original blood volume) containing lU/ml 75 WO 2008/137809 PCT/US2008/062584 apyrase (grade V, Sigma, St. Louis, Mo.). After incubation at 37 0 C for 15 minutes, the platelets are collected by centrifugation at 730 x g for 10 minutes and re-suspended at a concentration of 3 x 108 platelets/ml in Hepes-Tyrode's buffer (10 mM Hepes, 138 mM NaCl, 5.5 mM glucose, 2.9 mM KCl, 12 mM NaHCO 3 , pH 7.4) containing 0.1% bovine serum 5 albumin, 1 mM CaCl 2 and 1 mM MgCl 2 . This platelet suspension is kept >45 minutes at 37 0 C before use in aggregation assays. 2. [0292] For cuvette light transmittance aggregation assays, serial dilutions (1:3) of test compounds were prepared in 100% DMSO in a 96 well V-bottom plate (final DMSO 10 concentration in the cuvette was 0.6%). The test compound (3 pl of serial dilutions in DMSO) was pre-incubated with PRP for 30-45 seconds prior to initiation of aggregation reactions, which were performed in a ChronoLog aggregometer by addition of agonist (5 or 10 tM ADP) to 490 ptL of PRP at 37 C. In some cases, light transmittance aggregometry was performed using 490 pL of washed platelets (prepared as described above) at 37 0 C, and 15 aggregation was initiated by addition of 5 iiM ADP and 0.5 mg/ml human fibrinogen (American Diagnostics, Inc., Greenwich, Conn.). The aggregation reaction is recorded for ~ 5 mins, and maximum extent of aggregation is determined by the difference in extent of aggregation at baseline, compared to the maximum aggregation that occurs during the five minute period of the assay. Inhibition of aggregation was calculated as the maximum 20 aggregation observed in the presence of inhibitor, compared to that in the absence of inhibitor. IC 50 values were derived by non-linear regression analysis using the Prism software (GraphPad, San Diego, CA). 3. [0293] Inhibition of ADP-dependent aggregation was also determined in 96-well flat 25 bottom microtiter plates using a microtiter plate shaker and plate reader similar to the procedure described by Frantantoni et al., Am. J. Clin. Pathol. 94, 613 (1990). All steps are performed at room temperature. For 96-well plate aggregation using platelet-rich plasma (PRP), the total reaction volume of 0.2 ml/well includes 180 p of PRP (-3 x 108 platelets/ml, see above), 6 pl of either serial dilution of test compounds in 20% DMSO or 30 buffer (for control wells), and 10 pl of 20X ADP agonist solution (100 pM). The OD of the samples is then determined at 450 nm using a microtiter plate reader (Softmax, Molecular Devices, Menlo Park, Calif.) resulting in the 0 minute reading. The plates are then agitated 76 WO 2008/137809 PCT/US2008/062584 for 5 min on a microtiter plate shaker and the 5 minute reading is obtained in the plate reader. Aggregation is calculated from the decrease of OD at 450 nm at t=5 minutes compared to t=0 minutes and is expressed as % of the decrease in the ADP control samples after correcting for changes in the unaggregated control samples. IC 50 values were derived by non-linear 5 regression analysis. [0294] For 96-well plate aggregation using washed platelets, the total reaction volume of 0.2 ml/well includes in Hepes-Tyrodes buffer/0. 1% BSA: 4.5 x 107 apyrase-washed platelets, 0.5 mg/ml human fibrinogen (American Diagnostica, Inc., Greenwich, Conn.), serial dilutions of test compounds (buffer for control wells) in 0.6% DMSO. After ~ 5 minutes pre 10 incubation at room temperature, ADP is added to a final concentration of 2 tM which induces submaximal aggregation. Buffer is added instead of ADP to one set of control wells (ADP- control). The OD of the samples is then determined at 450 nm using a microtiter plate reader (Softmax, Molecular Devices, Menlo Park, Calif.) resulting in the 0 minute reading. The plates are then agitated for 5 min on a microtiter plate shaker and the 5 minute reading is 15 obtained in the plate reader. Aggregation is calculated from the decrease of OD at 450 nm at t=5 minutes compared to t=0 minutes and is expressed as % of the decrease in the ADP control samples after correcting for changes in the unaggregated control samples. IC 50 values were derived by non-linear regression analysis. 20 II. Inhibition of [3H]2-MeS-ADP Binding to Platelets 1. The ability of candidate molecules to inhibit the binding of [3H]2-MeS-ADP to the P2Y12 receptor on platelets was determined using a radioligand binding assay. [0295] Utilizing this assay the potency of inhibition of such compounds with respect to

[

3 H]2-MeS-ADP binding to whole platelets is determined. Under the conditions described in 25 11 (3) below, the binding of [ 3 H]2-MeS-ADP is solely due to the interaction of this ligand with the P2Y 12 receptor, in that all the specific binding measured in this assay is competable with a P2Y 1 2 antagonist (i.e., the specific binding is reduced to background levels by competition with an excess of P2Y 12 antagonist, with no competition of binding when a P2Yi antagonist is pre-incubated with the platelet preparation). [ 3 H]2-MeS-ADP binding 30 experiments are routinely performed with outdated human platelets collected by standard procedures at hospital blood banks. Apyrase-washed outdated platelets are prepared as follows (all steps at room temperature, if not indicated otherwise): 77 WO 2008/137809 PCT/US2008/062584 [0296] Outdated platelet suspensions are diluted with 1 volume of CGS and platelets pelleted by centrifugation at 1900 x g for 45 minutes. Platelet pellets are re-suspended at 3-6 x 109 platelets/ml in CGS containing 1 U/ml apyrase (grade V, Sigma, St. Louis, Mo.) and incubated for 15 minutes at 37 0 C. After centrifugation at 730 x g for 20 minutes, pellets are 5 re-suspended in Hepes-Tyrode's buffer containing 0. 1% BSA (Sigma, St. Louis, Mo.) at a concentration of 6.66 x 108 platelets/ml. Binding experiments are performed after >45 minutes resting of the platelets. 2. [02971 Alternatively, binding experiments are performed with fresh human platelets 10 prepared as described in section I (Inhibition of ADP-Mediated Platelet Aggregation in vitro), except that platelets are re-suspended in Hepes-Tyrode's buffer containing 0.1% BSA (Sigma, St. Louis, Mo.) at a concentration of 6.66 x 108 platelets/ml. Very similar results are obtained with fresh and outdated platelets. 3. 15 [02981 A platelet ADP receptor binding assay (ARB) using the tritiated potent agonist ligand [ 3 H]2-MeS-ADP (Jantzen, H. M. et al. (1999) Thromb. Hemost. 81:111-117) has been adapted to the 96-well microtiter format. In an assay volume of 0.2 ml Hepes-Tyrode's buffer with 0.1% BSA and 0.6% DMSO, 1 x 10' apyrase-washed platelets are pre-incubated in 96 well flat bottom microtiter plates for 5 minutes with serial dilutions of test compounds before 20 addition of I nM [ 3 H]2-MeS-ADP ([ 3 H]2-methylthioadenosine-5'-diphosphate, ammonium salt; specific activity 20-50 Ci/mmole, obtained by custom synthesis from Amersham Life Science, Inc., Arlington Heights, Ill., or NEN Life Science Products, Boston, Mass.). Total binding is determined in the absence of test compounds. Samples for nonspecific binding may contain 10 pM unlabelled 2-MeS-ADP (RBI, Natick, Mass.). After incubation for 15 25 minutes at room temperature, unbound radioligand is separated by rapid filtration and two washes with cold (4-8 'C) Binding Wash Buffer (10 mM Hepes pH 7.4, 138 mM NaCI) using a 96-well cell harvester (Minidisc 96, Skatron Instruments, Sterling, Va.) and 8 x 12 GF/C glassfiber filtermats (Printed Filtermat A, for 1450 Microbeta, Wallac Inc., Gaithersburg, Md.). The platelet-bound radioactivity on the filtermats is determined in a scintillation 30 counter (Microbeta 1450, Wallac Inc., Gaithersburg, Md.). Specific binding is determined by subtraction of non-specific binding from total binding, and specific binding in the presence of 78 WO 2008/137809 PCT/US2008/062584 test compounds is expressed as % of specific binding in the absence of test compound dilutions. IC 50 values were derived by non-linear regression analysis. [02991 In the table below, activity in the PRP assay is provided as follows: +++, ICso < 10 ptM; ++, 10 pM < IC 50 < 30 ptM. Activity in the ARB assay is provided as follows: +++, IC 50 5 < 0.05 pM; ++, 0.05 pM < IC 50 < 0.5 pM. Table 3: Example No. ARB Binding PRP Activity Example 2 +++ +++ Example 3 ++ ++ Example 6: Synthesis of [4-( 6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyll-5-chloro-thiophen-2-yl-sulfonylurea potassium salt (9a) 10 (amorphous form) H H \ i 0 O N 0 2M KOH (1.15 equiv) F 0 H3CN

THF-H

2 0 (2.5:1) N N 0 50 0 C, 0.5 h H H H K CI F N H3CN NO H H (9a) [03001 The free-acid, sulfonylurea, (7.0 g, 13.365 mmol) was suspended in THF/H 2 0 (55: 22 mL, ca. 2.5:1), and treated with 2M KOH (7.70 mL, 15.40 mmol, 1.15 equiv) drop wise, 15 over ca. 5 min. By the time the addition was over, a clear solution resulted. However, a solid precipitated out after <5 mins and the reaction mixture became a heavy suspension. This was heated in an oil-bath to 50 C, and the resulting clear viscous light brown solution was held at this temperature for 0.5 h. On cooling to rt., the title compound (9a)precipitated out. The 79 WO 2008/137809 PCT/US2008/062584 mixture was diluted with i-PrOH (250 mL, 3x the original reaction volume), stirred at rt. for 3h, and then filtered through a Buchner funnel to yield the title compound (9a) as a white solid. This was dried in a vacuum oven at 80 0 C to yield 7.20g (96%) of an amorphous solid. MS (negative scan): 521.7; 523.7. 5 Example 7: Conversion of the sulfonylurea (7a) to its amorphous sodium salt (10a) H H j\ C I N N, F O N S S 2N NaOH (1.0 equiv) H3CN

CH

3

CN-H

2 0 (1:1) N N O 7a rt., 1.0 h H N a Cl F , NI N H3C'N N 0 10a H H [03011 1-(5-chlorothiophen-2-ylsulfonyl)-3-( 4

-(

6 -fluoro-7-(methylamino)-2, 4-dioxo-1, 2 dihydroquinazolin-3(4H)-yl) phenyl) urea (3.0 g, 5.728 mmol) 7a was suspended in 10 CH 3

CN/H

2 0) (1:1; 70 mL) and was treated with 2N NaOH (2.90 mL, 5.80 mmol), dropwise. Within ca. 15 minutes, a clear solution resulted. After stirring for 1.0 h, the now light brown solution was lyophilized to afford the crude product as an amorphous solid 10a. MS (negative scan): 522.0; 524.0. 15 Example 8: Alternative preparation of amorphous form of the sodium salt [03021 Sodium salt 10a was suspended in isopropanol (100 mL) and refluxed for ca. 45 min, then hot filtered to yield a tan coloured solid, which is mostly the title compound by HPLC. The solid was suspended in CH 3 CN: EtOH (1:2) (100 mL) and refluxed for 45 mins, then hot filtered to afford 2.54 g of the title compound 10a as a tan coloured solid (99.7% 20 pure by analytical HPLC, long column). The filtrate was diluted with EtOH until the ratio of ACN:EtOH became (1:3) and it was let to stand at room temperature overnight. An additional crop of the title compound precipitated out to afford 210 mg of solid 10 a (purity: 99.7% by analytical HPLC, long column). 80 WO 2008/137809 PCT/US2008/062584 Example 9: Salt screen of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin- 3 -yl)-phenyll-5-chloro-thiophen-2-yl-sulfonylurea Primary Screen [0303] To 20 mg of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1, 4 -dihydro-2H-quinazolin-3 5 yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea in 3 mL of the various solvents, was added 1.1 eq. of the base in 1 mL solvent. The mixture was shaken for 2 hours and the solutions were left to evaporate down to half their volume to try to precipitate out the salt. The results are presented in Table 4 below, which shows the bases used for the screen. The solutions in THF evaporated down to solids very quickly and these were analysed by XRPD. Most 10 samples from THF were amorphous oily solids which were left to maturate at 50'C/ambient temperature. Any solutions that did not form a solid by evaporation had IPA added as an anti-solvent to induce solid to precipitate. Samples with IPA that did not precipitate were left to evaporate. As shown in Table 5 below, the solutions yielded some solids and some oils. Oils/emulsions and opaque liquids were left to maturate at 50'C/ambient in an 8 hour cycle 15 for several weeks. Microscopy and XRPD results showed some samples were crystalline but lack of a solid meant clear diffractograms could not be obtained. Solid samples (crystalline and amorphous) were then filtered, dried and then analyzed to judge their purity, crystallinity and stability. Solids were analysed by 'H NMR to confirm salt formation and analyzed by Ion Chromatography and TGA to obtain the stoichiometry of the salt. 81 WO 2008/137809 PCT/US2008/062584 Table 4: Primary Salt Screen Base Solvent MeCN/Water IPA Water DMSO THF Potassium hydroxide solution Partially crystalline Partially crystalline solution Partially crystalline not acid or base not acid or base _ not acid or base Sodium hydroxide solution Partially crystalline solution solution Partially crystalline not acid or base not acid or base Calcium acetate PartiallyWcrystaIIne Partially crystalline Weakly crystalline _______ matches free acid~; not acid or basebase \ bkly crystaintm L-lysine monohydrate solution Amorphous Aoposslto i Ammonium hydroxide solution Partially crystalline P ac l __________________ not acid or base 7moerphous solution Patlycrslin not acid or base Magnesium acetat Partially crystalline Prtiaij' ry~talline Icrtliie eee acid not acid or base catches free acid solutonYfr d'd L-arginine oil Amorphous Amorphous solution oil Tromethamine Amorphous' Amorph ous Partially'crystalline slto i matches fr ee acid slto i N-ethylglucamine Amorphous solution Partially crystalline solution oil notnacid or base N-methylglucamine solution gel Amorphous solution oil Potassium ethoxide solution (some ppt) Amorphous Arllycrs solution Weaklcrstalline matc eossIbaifrde id Sodium ethoxide <Anphui Partially crystalline N--ethy g ucamine noAm orph os solution Partially crystalline noo acd or base 82 WO 2008/137809 PCT/US2008/062584 Tables 5a and 5b: Characterisation results Table 5a Cation Solvent Physical XRPD of the XRPD of the dried 1H NMR state slurry sample Potassium MeCN/Water solution ppt formed on ppt drying hydroxide addition of IPA antisolvent Potassium MeCN/Water solution ethoxide Potassium IPA solid Partially crystalline, Partially crystalline, Shifts seen, IPA, hydroxide consistent with consistent with Form Water Form B B Potassium IPA solid Amorphous Partially crystalline, Shifts seen IPA, ethoxide comnistent with water Form B Potassium Water solid Partially crystalline, Partially crystalline, Shifts seen, water hydroxide consistent with consistent with Form Form C C Potassium Water solid Amorphous (small Partially crystalline, Shifts seen, water ethoxide particles) consistent with Form C Potassium DMSO solution hydroxide Potassium DMSO solution ethoxide Potassium THF solid Weakly crystalline, Weakly crystalline, Shifts seen THF, hydroxide Form D Form D water, DMF Potassium THF solid Weakly crystalline, Weakly crystalline Shifts seen, THF, ethoxide Form D Form D DMF, IPA and water Sodium MeCN/Water solution hydroxide Sodium MeCN/Watef solid Amorphous (small Partially crystalline, ethoxide particles) matches free acid Sodium IPA solid Weakly Crystalline, Weakly Crystalline, Shifts seen, IPA, hydroxide Form A Form A Water (trace THF, DMF) Sodium IPA solid Partially crystalline, Partially crystalline, Shifts seen IPA, ethoxide Form B Form B water, DMF Sodium Water solution hydroxide Sodium Water solid Amorphous Partially crystalline, ethoxide matches free acid Sodium DMSO solution hydroxide Sodium DMSO solution ethoxide Sodium THF solid Partially crystalline, Partially crystalline, Shifts seen, THF, hydroxide Form A Form A water, IPA Sodium THF solid Partially crystalline, Partially crystalline, Shifts seen THF, ethoxide Form A Form A water, DMF Calcium MeCN/Water solid Partially crystalline, Partially crystalline, acetate matches free acid matches free acid Calcium IPA solid Not Partially Crystalline, Form A ShifIs seen, IPA, acetate crystalline, Form A water (trace THF) Calcium Water solid Weakly crystalline Partially crystalline, No shifts seen, free acetate matches free acid acid Calcium DMSO solution acetate Calcium THF emulsion Partialily Partially crystalline, acetate crystalline, Form B Form B 83 WO 2008/137809 PCT/US2008/062584 Cation Solvent Physical XRPD of the XRPD of the dried 1H NMR state slurry sample L-lysine MeCNNater solution monohydrate L-lysine IPA solid Weakly crystalline, Weakly crystalline Shifts seen IPA, monohydrate Form A water L-lysine Water solid Amorphous Partially crystalline, monohydrate matches free acid L-lysine DMSO solution monohydrate L-lysine THF oil monohydrate Table 5b Cation Solvent Physical XRPD of the slurry XRPD of the dried state sample Ammonium MeCN/Water solution Crystalline, Form B Crystalline, Form B hydroxide Ammonium IPA solid Partially crystalline, Form Partially crystalline, Form hydroxide A A Ammonium Water solid Crystalline, Form B Crystalline, Form B hydroxide Ammonium DMSO solution hydroxide Ammonium THF solid Partially crystalline, Partially crystalline, hydroxide consistent with Form A consistent with Form A Magnesium acetate MeCN/Water solid Partially crystalline, Partially crystalline, matches free acid matches free acid Magnesium acetate IPA solid Partially crystalline, not Partially crystalline, form free acid or base change on drying Magnesium acetate Water solid Partially crystalline, Partially crystalline, matches free acid matches free acid Magnesium acetate DMSO solution Magnesium acetate THF solid sample evaporated so no Partially crystalline, slurry mixture of free acid and Mg acetate L-arginine MeCN/Water oil L-arginine IPA solid Amorphous Amorphous L-arginine Water solid Amorphous Amorphous L-arginine DMSO solution L-arginine THF oil Tromethamine MeCN/Water solid Amorphous (small Parlially crystalline, particles) matches free acid Tromethamine IPA solid Amorphous (small Partially crystalline, not particles) free acid or base Tromethamine Water solid Partially crystalline, Partially crystalline, matches free acid matches free acid Tromethamine DMSO solution Tromethamine THF solid Partially crystalline, Form A N-ethylglucamine MeCN/Water solid Amorphous (small Weakly crystalline particles) N-ethylglucamine IPA solution N-ethylglucamine Water solid Partially crystalline, not Insufficient solid from free acid or base filtering N-ethylglucamine DMSO solution N-ethylglucamine THF oil N-methylglucamine MeCN/Water solution N-methylglucamine IPA gel 84 WO 2008/137809 PCT/US2008/062584 Cation Solvent Physical XRPD of the slurry XRPD of the dried state sample N-methylglucamine Water solid Amorphous (small Weakly crystalline, particles) matches free acid N-methylglucamine DMSO solution N-methylglucamine THF oil Scale-up of salt forms [03041 A secondary evaluation of several salt forms was carried out using the methods described above on a 100 mg scale with the results summarized in the Table 6 and the Figures. 5 Table 6: Scale-up Characterization Cation Solvent Yield XRPD analysis of 'H NMR TGA DSC dry sample Potassium THF 100.30% Consistent with Shifts seen, salt 3.4% loss (32-87*C) Endotherm (onset hydroxide salt screen sample formation 7.8% loss (87-229*C) 25*C, 54.4J/g) (Form D), more confirmed, Residual Endotherm (onset crystalline water, IPA and THF 132"C, 13.6J/g) Form B as 2.8% loss (amb- Endotherm (onset 25* supplied (lot lot 150*C) 140"C, 118.7J/g) 01POR07a-01-30) Degradation onset ca. Endotherm (onset 240*C 276.8*C, 63J/g). Sodium THF 104.50% Consistent with Shifts seen, salt 2.1% loss (32-66*C) Endotherm (onset hydroxide Form A, more formation 7.5% loss (66-150*C) 33*C, 22.OJ/g) crystalline confirmed, Residual 4.4% loss (150-231*C) Endotherm (onset water, IPA and THF 1.6% loss (231-276*) 97'C, 17.8J/g) Endotherm (onset 162*C, 21.8J/g) Sodium IPA 104.20% Consistent with Shifts seen, salt 16.9% loss (32-222*C) Endotherm (onset hydroxide Form A, more formation 1.5% loss (222-271*C) 88*C, 89.2J/g) crystalline confirmed, Residual Endotherm (onset water, IPA and THF 256*C, 45.9J/g) Calcium IPA 124.70% Consistent with Shifts seen, salt 1.0% loss (31-71*C) Endotherm (onset acetate salt screen sample formation 8.2% loss (71-217*C) 25*C, 11.6J/g) (Form A), more confirmed, Residual 1.0% loss (217-264*C) Endotherm (onset crystalline water and IPA 125"C, 79.6J/g) Tromethamine IPA 88.60% Consistent with Shifts seen, salt 0.8% loss (31-68"C) Endotherm (onset salt screen sample formation 3.1% loss (68-176*C) 25*C, 17.6J/g) (Form A), more confirmed, ratio Endotherm (onset crystalline acid:base is 1:1.07 165*C, 43.7J/g) i.e. mono salt Endotherm (onset Residual water and 179*C, 3.4J/g) IPA Ammonium IPA 89.70% Consistent with Shifts seen, salt 1.0% loss (30-80"C) Endotherm (onset hydroxide Form A, similar formation 4.8% loss (80-165'C) 28*C, 16.1J/g) crystallinity confirmed, Residual 1.2% loss (165-183*C) Endotherm (onset water and IPA 1460C, 63.9J/g) Ammonium Water 96.60% Consistent with Shifts seen, salt 8.0% loss (31-115'C) Endotherm (onset hydroxide Form B, less formation 1.3% loss (115-173*C) 64"C, 190.9J/g) crystalline, some confirmed, Residual 3.8% loss (173-216*C) Endotherm (onset peak shifts to water 139'C, 16.7J/g) smaller 2theta Exotherm (onset 183*C, values 14.OJ/g) 85 WO 2008/137809 PCT/US2008/062584 [0305] Yields have been calculated based on an anhydrous mono salt. Solubility is the aqueous thermodynamic solubility, expressed as free base equivalents Sodium salts [0306] All samples scaled up well, in good yields (though some had residual solvent 5 associated with them) and good chemical purities. All samples were confirmed to be salts by H NMR. Both sodium salts are consistent with form A, which confirms reproducibility of form A from the THF solvent system. The IPA/sodium ethoxide method sometimes gave form B but on scale-up the powder pattern was different from both forms A and B. The sodium salts showed good solubility but were not stable to 40'C/75%RH for 3 days. 10 Characterization of Sodium from THF [0307] 'H NMR: Chemical shift seen, confirming salt formation. Residual solvents: Water, IPA, THF [0308] Purity by HPLC is 99.6A% 15 [03091 Ion Chromatography. Ratio acid: base is 1:0.92. When adjusted for solvent content acid:base 1:1.02 i.e. a mono salt [03101 Solubility. Solubility >10 mg/ml free base equivalent. pH of the clear solution (after shaking at 25'C for 24hours) = 8.76. The sample was a clear solution so there was no residue for analysis by XRPD 20 Characterization of Sodium salt from IPA [03111 'H NMR: Chemical shift seen, confirming salt formation. Residual solvents: Water, IPA, THF [03121 Purity by HPLC is 99.OA% 25 [03131 Ion Chromatography. Ratio acid: base is 1:0.92. When adjusted for solvent content acid:base 1:1.11 i.e. a mono salt 86 WO 2008/137809 PCT/US2008/062584 [0314] Solubility. Solubility = >10 mg/ml free base equivalent. pH of the clear solution (after shaking at 25 0 C for 24hours)= 9.06. The sample was a clear solution so there was no residue for analysis by XRPD. 5 Characterization of Calcium salt [0315] 1H NMR: Chemical shift seen, confirming salt formation. Residual solvents: Water, IPA [0316] Purity by HPLC is 98.8A% [03171 Ion Chromatography. Ratio acid: base is 1:0.76. When adjusted for solvent content 10 acid:base 1:0.84 [0318] Solubility. Solubility = 0.04 mg/ml free base equivalent. pH of the saturated solution (after shaking at 25'C for 24hours) = 7.36 [0319] XRPD of the residue showed a new XRPD pattern. 15 Characterization of Tromethamine salt [0320] 'H NMR: Chemical shift seen, confirming salt formation. Ratio of Tromethamine: free acid is 1.07:1 i.e. a mono salt with slight excess of tromethamine. Residual solvents: Water, IPA [0321] Purity by HPLC is 98.7A% 20 [0322] Solubility. Solubility = 2.4 mg/ml free base equivalent. pH of the saturated solution (after shaking at 25'C for 24 hours) = 8.90 [0323] XRPD of the residue showed a new XRPD pattern (sample has become almost amorphous) 25 Characterization of Ammonium salt from IPA [03241 'H NMR: Chemical shift seen, confirming salt formation. Residual solvents: Water, IPA. 87 WO 2008/137809 PCT/US2008/062584 [0325] Purity by HPLC is 98.1A% [03261 Ion Chromatography. Ratio acid: base is 1:0.52. When adjusted for solvent content acid:base is 1:0.56 i.e. a hemi salt. [03271 Solubility. Solubility = 2.3mg/ml free base equivalent. pH of the saturated solution 5 (after shaking at 25'C for 24hours) = 8.80. XRPD of the residue showed a new XRPD pattern, which is similar to form B of the Ammonium salt Characterization of Ammonium salt from water [0328] 1H NMR: Chemical shift seen, confirming salt formation. Residual solvents: Water 10 [03291 Purity by HPLC is 98.1A% [0330] Ion Chromatography. Ratio acid: base is 1:0.50. When adjusted for solvent content acid:base is 1:0.56 i.e. a hemi salt [0331] Solubility. Solubility = 1.9 mg/ml free base equivalent. pH of the saturated solution (after shaking at 25'C for 24hours) = 8.08 15 [0332] XRPD of the residue showed no changes to the XRPD pattern. Example 9: Preparation of polymorph form A of potassium salt by recrystallization [0333] Recrystallization: The crude product can be recrystallized either from MeOH or MeOH/EtOH (3:1) by first heating to reflux to dissolve, and then cooling to room 20 temperature to precipitate. [0334] Recrystallization From MeOH: 1.Og of the potassium salt was suspended in MeOH (150 mL) and heated to reflux for 0.5h, resulting in an almost clear solution. This was then hot filtered through a Buchner funnel. The clear filtrate on standing at room temperature deposited a white solid. This was stirred overnight and then collected by filtration through a 25 Buchner funnel. The solid product was rinsed with EtOH (2 x 4.0 mL) and dried in a vacuum oven at 80 0 C for 20h to yield 740 mg of a colorless solid. The mother liquor yielded more title compound on concentration to ca. one-third of the original volume. 88 WO 2008/137809 PCT/US2008/062584 [0335] Recrystallization from EtOI/MeOH: 1.0 g of the potassium salt was suspended in the solvent mixture EtOH/MeOH (1:3) (200 mL), and heated to reflux for 0.5 h resulting in an almost clear solution. This was then hot filtered through a Buchner funnel. The clear filtrate on standing at room temperature deposited a colorless solid. This was collected by 5 filtration through a Buchner funnel. The solid product was rinsed with EtOH and dried in vacuum oven at 80 C for 20h to give a white solid. The mother liquor yielded more title compound upon concentration to ca. one-third of the original volume. [0336] Recrystallization of Form B From MeOH: [ 4

-(

6 -fluoro-7-methylamino-2,4 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea 10 potassium salt (C5 009, 500mg) was charged to a 1 00ml round bottomed flask and methanol (67ml) added. The suspension was heated with magnetic stirring to reflux for 30 minutes. Dissolution did not occur therefore two further portions of methanol (20ml) were added over the course of 1 hour. Dissolution had still not occurred and the limits of the vessel had been reached. The suspension was cooled to ambient then filtered under vacuum and the solid 15 (crop 1) was oven dried at 45*C under vacuum. A portion of the mother liquors (ca. 20ml) was concentrated under vacuum to dryness (crop 2) and the remaining mother liquors were concentrated to ca. 30 ml. Within minutes, it was observed that the flask became very cold and much solid precipitated (crop 3). This suggested that the solution was not saturated before concentration. 20 [0337] XRPD analysis of all three crops showed that only crop 3 resembled the form A powder pattern exactly. It was hypothesised that crops 1 and 2 were solids in transition between form B and form C, as crop 1 appeared to contain the 5.2 2Theta peak that is distinctive of form B, and crop 2 did not have the form B peak, but neither did it have the 4.8 2Theta form A peak. A single crystal from the liquors of crop 3 confirmed that form A is a 25 2.5 hydrate where one molecule of water is coordinated to the potassium and for each [4-(6 fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea potassium salt moiety, 1.5 molecules of water are hydrogen bonded. It is thought that the ease of movement of the hydrogen bonded water determines whether the peak at 4.8 2Theta is observed or not. The structure details can be found below 30 section 10. 89 WO 2008/137809 PCT/US2008/062584 Example 10: Preparation of form B of potassium salt by recrystallization [0338] Recrystallization: The crude product can be recrystallized from EtOH/H 2 0 (91:9) or a small volume of MeOH by first heating to reflux to dissolve, and then cooling to room 5 temperature to precipitate. [0339] Recrystallization from EtOH/H 2 0: 1.Og of the potassium salt was suspended in EtOH (190 mL) and heated to reflux. To the heavy suspension was added H 2 0 (18.0 mL) dropwise, resulting in a clear colorless solution. On cooling to room temperature, the title compound precipitated out as a white solid. It was collected by filtration through a Buchner 10 funnel, and rinsed with EtOH (2 x 4.0 mL). This was dried in vacuum oven at 80 'C for 20 h, to give 650 mg of a colorless solid. The mother liquor yielded more title compound upon concentration to ca. one-third of the original volume. [0340] Large Scale recrystallization from small volume of MeOH: 6.6g of the potassium salt was suspended in MeOH (30 mL) and heated to reflux for 5hr, the solid did 15 not completely dissolve in this volume of methanol. After cooling the solid was filtered and rinsed with iPrOH. The solid was dried in vacuum oven at 80'C for 20 h, to give 6.2 g of colorless solid, which after characterization was shown to be form B. [0341] Form B has been shown to be quite stable towards moisture and temperature. The API has been exposed to 75%RH/40' C for up to 6 months with no change in solid state. 20 Example 11: Polymorphism Studies on Form B of the potassium salt [0342] The propensity of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt form B to form polymorphs was studied. Form B(a hemi-hydrate) was slurried in a range of solvents (neat 25 and mixtures). The solvents were chosen based on their pharmaceutical acceptability and also a range of functional groups and polarities such as alcohols, ethers and esters. To encourage hydrate formation, aqueous mixtures were also chosen. The solvents used are detailed in Table 7. 90 WO 2008/137809 PCT/US2008/062584 Table 7 Ambient polymorphism experiments solvent volume/pl XRPD acetone 500 Form B acetone/water 500 change in pattern THF 500 Form B THF/water 500 mixture of Form B and LJC-225 001-2 pattern EtOH 500 Form B EtOH/water 500 Form B DCM 500 Form B DCM/MeOH (9:1) 500 Form B MtBE 500 Form B 2-MeOEtOH 500 this solvent dissolved K salt 2-MeOEtOH/water 500 Form B dioxane 500 Form B dioxane/water 500 Form B MEK 500 Form B IPA 500 Form B IPA/water 500 Form B EtOAc 500 Form B EtOAc/heptane 500 Form B MeCN 500 Form B MeCN/water 500 Form B water 500 Form B [0343] Approximately 50mg of [4-(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H 5 quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt, form B was suspended in ten volumes of the solvents detailed in table 7 and stirred at ambient for two hours. It was observed that 2-methoxyethanol was the only solvent that dissolved the potassium salt. The suspensions were filtered under vacuum and analysed by XRPD. Most of the solids remained as form B, with a 1:1 acetone/water mixture leading to a subtle change 10 in solid form. The 1:1 tetrahydrofuran/water mixture generated a mixture of that subtly different form and form B. [0344] To all the form B samples a further aliquot of five volumes of appropriate solvent was added and the suspensions were slurried at 50*C for 4 hours then cooled to ambient for 4 hours. This cycle was repeated for a total of 24 hours, after which time the suspensions were 15 filtered under vacuum and analysed by XRPD. The results are detailed in Table 8. 91 WO 2008/137809 PCT/US2008/062584 Table 8 Heat cycle polymorphism experiments solvent volume/pl (further XRPD portion) acetone 250 amorphous acetone/water 250 N/A THF 250 amorphous THF/water 250 N/A EtOH 250 family 2 EtOH/water 250 family 1 DCM 250 -family 2 DCM/MeOH (9:1) 250 family 2 MtBE 250 Form B 2-MeOEtOH 250 N/A 2-MeOEtOH/water 250 family 3 dioxane 250 family 4 dioxane/water 250 family 5 MEK 250 family 2 IPA 250 family 2 IPA/water 250 family 1 EtOAc 250 family 2 EtOAc/heptane 250 family 2 MeCN 250 Form B MeCN/water 250 family 1 water 250 Form B [0345] The changes observed in solid form were only slightly different from form B. For this reason, the different phases were categorised into families rather than given definitive 5 form names until further analysis had confirmed them as being different. [0346] In order to characterise the materials, a range of techniques (DSC, VTXRPD and 'H NMR) were carried out. Identification of family 1 10 [0347] The powder pattern of family I was the best match for form B of all the families isolated. The only differences appeared to be due to reduction in resolution (probably due to the instrument used). To confirm that this was the case thermal analysis was carried out. The DSC showed that the form B starting material melted slightly lower than the family 1 sample. To deduce if this was due to impurities, purity analysis was carried out on both samples. 92 WO 2008/137809 PCT/US2008/062584 [0348] The purity analysis measured the family 1 sample to be 99.8 area % and the form B starting material to be 99.9%. Purity was therefore ruled out as a reason for the difference. It was decided to carry out a VT XRPD experiment to deduce what the desolvated phase was. However, the solid when reanalysed had converted completely to form B. Family 1 was 5 therefore not re-investigated. Identification of Family 2 [0349] The phase labelled family 2 was isolated from many of the solvent systems used. In order to deduce whether or not the phase was a hydrate, thermal analysis was carried out. 10 The DSC experiment showed an endotherm suspected to be associated with a desolvation from ambient to ca. 102*C. This desolvated phase then melted at 281 'C. Karl Fischer analysis confirmed 3.4 % water content which is equivalent to 1.1 moles. To obtain further sample for the stabilities studies, a further aliquot of the original suspension was filtered. However, the XRPD showed the distinctive 5.2 2Th peak which was indicative that the 15 sample was changing to form B. A DSC experiment was ran to confirm the melting point, and it appeared that the sample was a mixture of form B and the mono hydrate, as the melting point had been reduced almost to that of form B at 279'C from 281 0 C. Identification of family 3 20 [0350] This solid form was isolated from 2-MeOEtOH/H 2 0 (1:1), as were single crystals generated in a separate experiment. The single crystal structure was solved as being a hemi 2-methoxy ethanol solvate, hemi hydrate and it was found that the calculated powder pattern from the data was very close to the actual pattern of form B. The structure showed that the water molecules were in the coordination sphere of the potassium. However, the 2-Methoxy 25 ethanol was interacting via hydrogen bonding. It was thought that the 2-methoxy ethanol could pass in and out of the structure without causing any change to it, i.e. resulting in a de solvated solvate, hence the similar powder patterns. 93 WO 2008/137809 PCT/US2008/062584 Identification of Family 4 [0351] The solid labelled as family 4 was the only solid isolated of this form. The DSC analysis indicated aesolvation fr-abroad endoterm hat occurred from an onset of 25'C to ca. 130*C. After this transition the trace was representative of an amorphous phase. It was 5 hypothesised if this form was in fact a solvate that de-solvated to an amorphous phase. To confirm this, a VT-XRPD experiment was carried out. [03521 The polymorphism screen concluded that form B (a hemi hydrate) showed propensity for further hydration or solvation. It was also noted that when further solvated by 2-methoxy ethanol, the solvent filled channels (detailed below). 10 2-Methoxy ethanol/water crystallisations [0353] A number of re-crystallisations were carried out using 2-methoxy ethanol and water as co-solvent as it had been deduced that 2-methoxy ethanol was the only solvent other than dimethylsulfoxide that dissolved [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H 15 quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt. The following reactions were carried out: Table 9 Solvent system Recrystallisation Experimental RPD results MR conditions observations results 2- Form B Solid dissolved on Has the same I MeOEtOH/H 2 0 suspended in 10 heating, but powder pattern (1:1) volumes and material as form B, heated with crystallized in although stirring to 93'C minutes without suspected to be with magnetic cooling required. an isostructrual stirring. 2-methoxy ______________ethanol solvate. 2- Form B Solid dissolved on MeOEtOH/H 2 0 suspended in 20 heating, but did Not applicable (60:40) volumes and not crystallize on heated to 70'C cooling. Oil with magnetic observed after 6 stirring days. 2- Form B Solid dissolved on Very close to 0.68 moles of MeOEtOH/H 2 0 suspended in 20 heating and form B, but 2-methoxy (1:1) volumes and crystallized on suspected to be ethanol heated to 73'C cooling, an isostructrual integrated. 94 WO 2008/137809 PCT/US2008/062584 with magnetic 2-methoxy Unstable stirring. ethanol solvate. solvates containing slightly different amounts of 2 methoxy ethanol 2- Form B Solid dissolved on Very close to 0.49 moles of MeOEtOH/H 2 0 suspended in 15 heating and form B, but 2-methoxy (60:40) volumes and crystallised on suspected to be ethanol heated to 73'C cooling. an isostructrual integrated. with magnetic 2-methoxy Unstable stirring. ethanol solvate. solvates containing slightly different amounts of 2 methoxy ethanol [0354] In order to confirm that the desolation of the 2-methoxy ethanol solvate to the hemi hydrate (that has been called form B to date) does not cause a significant change in the structure, and hence the powder pattern, a VT XRPD was carried out and the solid re 5 analysed by eH NMR. It was deduced that 2-methoxy ethanol/water combinations could not generate any form other than a 2-methoxy ethanol solvate of the hemi hydrate form B. It was therefore ruled out as a potential recrystallisation solvent due to it being regarded as a class 11 (ICH guidelines) solvent and therefore having residual level limits of 50ppm. 10 Potassium salt formation from [4-(6-fluoro-7-methylamino-2,4-dioxo-1 ,4-dihydro-2H quinazolin-3-yl)-phenyl] -5-chloro-thiophen-2-yl-sulfonylurea free acid [03551 A selection of solvents and aqueous solvent combinations that gave rise to subtle differences from form B in the polymorphismd screen were chosen as reaction solvents for generating the potassium salt from the free acid. The following experiments were carried 15 out: 95 WO 2008/137809 PCT/US2008/062584 Table 10 Experimental observations and results Solvent Experimental Experimental Observations Observations - XRPD conditions observations after ca. 5 on cooling to result minutes at ambient 50 0 C Acetone/water Free acid KOH added to Suspension Suspension Form B (1:1) suspension suspensions. heated to Most solid 50'C with dissolved ethanol stirring in 10 KOH added to Suspension Suspension Form B volumes then suspensions. KOH (1.0 equ Suspension Ethanol/water as IM in KOH added to Suspension Suspension Form B (1:1) H 2 0) added, suspensions. Most solid dissolved dioxane KOH added to Suspension Suspension New suspensions- pattern Suspension Dioxane/water KOH added to Solution Solution (1:1) suspensions. Most solid dissolved [0356] The four suspensions were filtered under vacuum and air dried. XRPD analysis was 5 then carried out. The sample from dioxin/water was discarded after one week as a brown oil was present. The solid from dioxane that gave the new powder pattern was fully characterised and deduced to be a 1,4-dioxane solvate with two equivalents of solvent to [4

(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro thiophen-2-yl-sulfonylurea. 10 [03571 The experiments carried out have shown that when starting with form B (dried of weakly bound solvent to a hemi hydrate), the solid further hydrates to a mono hydrate or solvates with certain solvents. The solvents fill a channel which therefore causes no change in structure when the solvent molecules vacate the spaces. For this reason, techniques other than XRPD alone are needed to deduce the actual form isolated. For further development of 15 form B, it must be confirmed that the material has been dried sufficiently to the hemi hydrate. No anhydrous forms of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3 yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt were identified. 96 WO 2008/137809 PCT/US2008/062584 Example 12: Preparation of form C of potassium salt by wet granulation [0358] A change in solid phase from form B was identified when wet granulation was carried out. Thus grinding form B of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt with 75% and 5 90% w/w water using mortar and pestle followed by heating at 40'C overnight results in conversion to either an amorphous form or a new form -form C of [4-(6-fluoro-7 methylamino-2,4-dioxo- 1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl sulfonylurea potassium salt. Form C has XRPD and DSC properties which are different from forms A and B of [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3-yl) 10 phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt. This new form also resulted from a wet granulation process where the API was mixed with excipients including Avicel, triacyl citrate, and water in a low shear granulator followed by extrusion and spherinization. In addition, this new form was possible to make in aqueous slurry when stored at ambient room temperature or in a refrigerator (2-8'C) for prolonged periods, i.e., 3 days. 15 [0359] The sample (primarily referred to as form C) was characterised by cation chromatography to confirm that the potassium salt was intact. The measurement confirmed 0.92 equivalents of potassium to [ 4 -(6-fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea, which was corrected for solvent content deduced by TGA. This new form C was subsequently identified to be a 20 hemi-potassium salt of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H-quinazolin-3 yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea. Table 11 Aqueous solubility measurements of form C Thermodynamic solubility in pH water/mg.ml 4.5 8.7 4.5 8.8 25 [03601 On an 800mg scale and 90% volume of water, form B was ground in a glass mortar with 90%volume of water with both phosphate buffer (pH 7.4 made form H 3

PO

4 and KOH) and DI water for between five and ten minutes. Samples were reanalysed by XRPD post grinding. 97 WO 2008/137809 PCT/US2008/062584 Table 12 Manual grinding experiments Experiment XRPD result Form B ground in a large Form B glass mortar with 90% volume of water for ca. 5 minutes. An aliquot taken and stored at 4'C for 4 days. Form B ground in a large Form B glass mortar with 90% volume of water for ca. 5 minutes. The paste was spread on a glass slide and air dried. A further 90% volume of Form B (much reduced water added to the bulk crystallinity) sample and ground for ca. 5 minutes. The paste was spread on a glass slide and dried at 45'C. A further 90% volume of Very close in pattern to form water added to the bulk C sample and ground for ca. 5 minutes. The sample stored at 45XC.RPDres [03611 The conclusion was that if form B was ground sufficiently to break down the lattice 5 and the amorphous phase was in the presence of water, it would hydrate to form C. To gain further information on the relative stabilities of form B and form C a number of experiments were set up involving 1: 1 mixtures of the solids. Qualitative relative stability studies 10 [03621 The relative stability of form A (a dihydrate) with form B and form C was studied. Qualitative relative stability studies carried out on form B and form C [0363] Approximately a 1: 1 ratio of form B and form C were lightly ground together in an agate mortar and a powder pattern was obtained. The following experiments were carried out on the mixture. 98 WO 2008/137809 PCT/US2008/062584 Table 13 Relative stability experiments Experiment XRPD result XRPD result after 1 day after 4 days The mixture was The sample was Solid had suspended in an emulsion crystallised from water (500[l) therefore the dried and stirred pipetted onto a emulsion to be magnetically. glass slide to form C dry. The mixture was Mixture Mixture stood in an atmosphere of 75% RH at 25'C. The mixture was Mixture Mixture stood in standard lab conditions with nothing added to it. _ [0364] These results supported that it was the amorphous potassium salt that crystallized as 5 form C. Form C from Form B [03651 In order to deduce if there was a robust method of converting form B to form C, a series of experiments were carried out using different lots of [4-(6-fluoro-7-methylamino-2,4 10 dioxo-1,4-dihydro-2H-quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt. The differences between lots were in particle size. The experiments were set up, the suspensions were filtered and washed with water and the results are detailed in Table 14. Table 14 Experimental procedure and results PRT 128 k Amount of Volume of Temperature of XRPD salt salt/mg H20 suspension/ C result post filtration not milled ca. 50 3.6 ambient mixture of B and C not milled ca. 50 3.6 4 mixture of B 99 WO 2008/137809 PCT/US2008/062584 and C not milled ca. 50 3.6 50 B not milled ca. 50 5 ambient B not milled ca. 50 5 4 B milled ca. 50 5 ambient mixture of B and C milled ca. 50 5 4 mixture of B and C milled ca. 50 5 50 mixture of B and C [0366] From the nine experiments carried out, eight were confirmed as form B or mixtures of form B and C and one led to single crystals that were of sufficient quality for diffraction. The crystal structure was solved as a hemi potassium salt which was hydrated. The level of 5 hydration was difficult to confirm due to the water being held in channels that allowed for easy desolvation. It is currently thought that at full occupancy 3 moles of water are present (see below for details). Qualitative relative stability studies carried out on form A and form C 10 [03671 Approximately a 1:1 ratio of form A and the form C were lightly ground together in an agate mortar and a powder pattern was obtained. Table 15 Relative stability experiments Experiment XRPD results after 4 days Form A/C mixture exposed No change from mixture to a 40'C/75%RH atmosphere Form A/C mixture stored in a No change from mixture 60'C/75%RH atmosphere for 5 days 15 [03681 The stressing conditions caused no conversion to either form. 100 WO 2008/137809 PCT/US2008/062584 Example 13: Single crystal X-ray diffraction studies [0369] Four samples were submitted for single crystal X-ray diffraction studies. The resulting structure analyses are provided throughout the rest of this section. Table 16 Single crystal structure of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H 5 quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt hemi 2-methoxy ethanol solvate, hemi hydrate Molecular formula

C

21

.

50

H

1 9 ClFKN 5 0 6

,

5 oS 2 Molecular weight 609.09 Crystal system Monoclinic Space group C2/C a 33.3580(5)A, a 90, b 15.093(3) A, 3 92.0408(7) , c 20.0081(4)A, 90 V 10067(2)A Z 16 De 1.607 g cm' 0.542 mm Source, 2A Mo-Kcx, 0.71073 A F(OOO) 4992 T 120(l) K Crystal Colourless, 0.4 x 0.4 x 0.05 mm Data truncated to 0.80 A Omax 22.44* Completeness 99.9% Reflections 26301 Unique reflections 10284 Rin _ 0.0525 [0370] The structure solution was obtained by direct methods, full-matrix least-squares 10 refinement on F 2 with weighting w- = 72(F,2) + (0.0925P) 2 + (20.OOOOP), where P =

(F

0 2 +2F 2 )/3, anisotropic displacement parameters, no absorption correction. Final wR 2 {Ey[w(F"2-Fe2)2]/E[w(FO2)2] m}= 0.1621 for all data, conventional R, = 0.0514 on F values of 7471 reflections with FO > 4oy( FO), S = 1.002 for all data and 708 parameters. Final A/a(max) 0.005, A/a(mean), 0.000. . 15 Table 17. Single crystal structure of mono acetonitrile solvate, hemi hydrate Molecular formula

C

21

.

50

H

1 9 ClFKN 5 0 6

.

5 oS2 Molecular weight 609.09 Crystal system Monoclinic Space group C2/c a 33.6106(5)A, a 900, 101 WO 2008/137809 PCT/US2008/062584 b 15.0902(3) A, p 91.8800(10) c 20.1282(3)A, y 90 V 10203.3(3) A 3 Z 16 Dc 1.586 g cm' p _ 0.535 mm' Source, X Mo-Ka., 0.71073 A F(OOO) 4992 T 120(1) K Crystal colourless, 0.4 x 0.4 x 0.05 mm Data truncated to 0.80 A Omax 22.44 Completeness 99.9% Reflections 45568 Unique reflections 10424 Rint 0.0679 [0371] The structure solution was obtained by direct methods, full-matrix least-squares refinement on F 2 with weighting w- = C2(F 0 2) + (0.1 000P) 2 + (0.0000P), where P =

(F

0 2 +2F 2 )/3, anisotropic displacement parameters, no absorption correction. Final wR 2 5 {E[w(F 2 -Fe 2

)

2 ]/y[w(F 2

)

2 ]1/ 2 } = 0.1808 for all data, conventional R I = 0.0567 on F values of 7073 reflections with F, > 4a( F,), S = 1.154 for all data and 721 parameters. Final A/c(max) 0.003, A/u(mean), 0.000. Table 18 Single crystal structure of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H 10 quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt 2.5 hydrate (form A) Molecular formula C 20

H

20 ClFKN 5

O

7

.

5 oS2 Molecular weight 608.08 Crystal system Monoclinic Space group P2 1 /n a 21.1534(5) A, c 900, b 6.9137(2)A, p 93.774(2)0, c 34.8001(11)A, 90 V 5078.4(2)A Z 8 De 1.591 g cm' p _ 0.54 mm' Source, X Mo-Ka, 0.71073 A F(OOO) 2496 T 120(l) K Crystal colourless prism, 0.16 x 0.12 x 0.05 mm 102 WO 2008/137809 PCT/US2008/062584 Omax 22.47 Completeness 91.5% Reflections 12824 Unique reflections 6056 Rin ___ 0.0497 [03721 The structure solution was obtained by direct methods, full-matrix least-squares refinement on F 2 with weighting w-1 = a2(F2) + (0.1 000P)2 + (0.0000P), where P = (Fj2+2F 2 )/3, anisotropic displacement parameters, no absorption correction. Final wR 2 = {E[w(F 2 -Fe 2

)

2 ]/X[w(F 2

)

2 ]/2} = 0.2072 for all data, conventional R 1 = 0.0636 on F values of 5 4777 reflections with F, > 4a( F), S = 1.493 for all data and 678 parameters. Final A/a(max) 0.01, A/a(mean), 0.001. Table 19 Single crystal structure of [ 4

-(

6 -fluoro-7-methylamino-2,4-dioxo-1,4-dihydro-2H quinazolin-3-yl)-phenyl]-5-chloro-thiophen-2-yl-sulfonylurea potassium salt hemi-potassium 10 salt, hydrate, (form C) Molecular formula C20HI 4 CIFKo.soN 5 OsS 2 .xH 2 0 (x = ca. 5) Molecular weight 542.48 Crystal system Triclinic Space group P-1 a 11.2838(6)^, a 117.623(4)*, b I1.4461(6)^, P 94.376(3), c 11.7629(7)k, y 98.599(3)* V 1312.43(13)A 3 Z 2 D, 1.373g.cm-1 p_ 0.429mm Source, X Mo-Ka, 0.71073^ F(OOO) 553 T 180(2)K Crystal colourless plate, 0.12 x 0.12 x 0.02mm Data truncated to 0.80A Oma 22.440 Completeness 99.1% Reflections 9120 Unique reflections 3370 Rint 0.0577 [03731 The structure solution was obtained by direct methods, full-matrix least-squares refinement on F 2 with weighting w-1 = a 2

(FO

2) + (0.1500P) 2 + (3.5000P), where P = 15 (F 2 +2Fe 2 )/3, anisotropic displacement parameters, no absorption correction. Final wR 2 = {Z[w(F,'2-Fe2)2]/y[W(Fo2 2] m}= 0.2571 for all data, conventional R I = 0.0778 on F values of 103 WO 2008/137809 PCT/US2008/062584 2459 reflections with Fo > 4a( FO), S = 1.069 for all data and 368 parameters. Final A/a(max) 0.004, A/o(mean), 0.000. Final difference map between +1.143 and -0.685e.A-. Example 14: Preparation of polymorph form D of potassium salt by recrystallization 5 [0374] 'H NMR: Chemical shifts confirm salt formation. [0375] Residual solvents: Water, IPA, THF. [0376] Purity by HPLC is 98.8A%. [0377] Ion Chromatography. Ratio acid: base is 1:0.89. When adjusted for solvent content acid:base is 1:1.0 i.e. a mono salt 10 [0378] Aqueous Thermodynamic Solubility. Solubility = 2.7mg/ml free base equivalent. pH of the saturated solution (after shaking at 25'C for 24hours) = 9.36. XRPD of the residue showed a new XRPD pattern. Method: 40 volumes of THF was added to 100mg of free acid at room termperature. This was then heated to 50'C for 2 hours and cooled at 4C slowly. The solid was filtered and 15 dried in a cauum oven at 25*C. The solid was confirmed to the the mono postassium salt by ion chromatography. [0379] Although the foregoing invention has been described in some detail by way of illustration and example for purposes of clarity of understanding, one of skill in the art will appreciate that certain changes and modifications may be practiced within the scope of the 20 appended claims. In addition, each reference provided herein is incorporated by reference in its entirety to the same extent as if each reference was individually incorporated by reference. 104

Claims (8)

1. A salt comprising a compound Formula I: F /0 0 C1 HN N S H3C HN 0 0-N 2 0 3 4 and an ion selected from the group consisting of sodium, potassium, 5 calcium, L-lysine, ammonium, magnesium, L-arginine, tromethamine, N-ethylglucamine and 6 N-methylglucamine. 1 2. The salt of claim 1, wherein the ion is potassium. 1 3. The salt of claim 1, wherein the ion is sodium. 1 4. The salt of claim 1, wherein the ion is calcium. 1 5. The salt of claim 1, wherein the ion is L-lysine. 1 6. The salt of claim 1, wherein the ion is ammonium. 1 7. The salt of claim 1, wherein the ion is magnesium. 1 8. The salt of claim 1, wherein the ion is L-arginine. 1 9. The salt of claim 1, wherein the ion is tromethamine. 1 10. The salt of claim 1, wherein the ion is N-ethylglucamine. 1 11. The salt of claim 1, wherein the ion is N-methylglucamine. 1 12. A salt having the formula: F HN / \ 0 0 K+ H,C HNN 2 N-O N N \ 20 105 WO 2008/137809 PCT/US2008/062584 3 in a crystalline solid form C characterized by at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 20b; and 6 (ii) a DSC scan substantially in accordance with the DSC pattern shown in 7 FIG. 25. 1 13. The salt of claim 12 in a crystalline solid form C characterized by an 2 X-ray powder diffraction pattern substantially in accordance with FIG. 24. 1 14. The salt of claim 12 in a crystalline solid form C characterized by a 2 DSC endotherm onset at about 56 C. This is true, although the endotherm shows 3 dehydration, so the remaining product is no longer form C as heating changes the sample. 4 This is the same for all hydrated species in this patent. 5 1 15. A salt having the formula: F 0 0 K+C HC / HN H ON S C 2 0 3 in a crystalline solid form D characterized by at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 26 or 27; and 6 (ii) a DSC scan substantially in accordance with the DSC pattern shown in 7 FIG. 29. 1 16. The salt of claim 15 in a crystalline solid form D characterized by an 2 X-ray powder diffraction pattern substantially in accordance with FIG. 26. 1 17. The salt of claim 15 in a crystalline solid form D characterized by a 2 DSC with endothermic events onset at about 54'C and at about 132 0 C. 106 WO 2008/137809 PCT/US2008/062584 1 18. A salt having the formula: F HN0 Na+ CI / - S H , C NO HN 2 0 3 in a crystalline solid form A which provides at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 30; and 6 (ii) a DSC scan substantially in accordance with FIG. 33. 1 19. The salt of claim 18 in a crystalline solid form A characterized by an 2 X-ray powder diffraction pattern substantially in accordance with FIG. 30. 1 20. The salt of claim 18 in a crystalline solid form A characterized by a 2 DSC with endothermic events at about 33*C, 97'C and 162 C. 1 21. A salt having the formula: F 0 0 Na+ C] H,C / HN- HNN H 3 NH HN 2 0 3 in a crystalline solid form B which provides at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 35; and 6 (ii) a TGA scan substantially in accordance with FIG. 36. 1 22. The salt of claim 21 in a crystalline solid form B characterized by an 2 X-ray powder diffraction pattern substantially in accordance with FIG. 35. 107 WO 2008/137809 PCT/US2008/062584 1 23. A salt having the formula: F 0 0 Na+ C1 / N S HC H , NN O N H HN 0 0 2 3 in a crystalline solid form C which provides at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 20a. 1 24. The salt of claim 23 having a crystalline form A which provides an X 2 ray powder diffraction pattern substantially in accordance with FIG. 20a. 1 25. The salt of claim 23 in a crystalline solid form C characterized by a 2 DSC endotherm onset at about 80 C. 1 26. A salt having the formula: F Ca C HN H HN /N H 0 0 2 0 3 in a crystalline solid form A which provides at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 38; and 6 (ii) a DSC scan substantially in accordance with FIG. 42. 1 27. The salt of claim 26 having a crystalline form which provides an X-ray 2 powder diffraction pattern substantially in accordance with FIG. 38. 108 WO 2008/137809 PCT/US2008/062584 1 28. The salt of claim 26 in a crystalline solid form A characterized by a 2 DSC endotherm onset at about 125 C. 1 29. A salt having the formula: F HNTro H3C H /N NH HN_ 0 0 2 0 3 in a crystalline solid form A which provides at least one of: 4 (i) an X-ray powder diffraction pattern substantially in accordance with FIG. 5 43; and 6 (ii) a DSC scan substantially in accordance with FIG. 47. 1 30. The salt of claim 29 having an amorphous form which provides an X 2 ray powder diffraction pattern substantially in accordance with FIG. 43. 1 31. The salt of claim 29 in a crystalline solid form A characterized by a 2 DSC endotherm onset at about 166 0 C. 1 32. The salt of claim any of the preceding claims, that is in an isolated and 2 purified form. 1 33. A pharmaceutical composition comprising a therapeutically effective 2 amount of a compound according to claim 1 and a pharmaceutically acceptable vehicle or 3 carrier. 1 34. The pharmaceutical composition of claim 33, wherein the compound in 2 the composition is in at least one solid form. 1 35. The pharmaceutical composition of claim 34, wherein the composition 2 is selected from the group consisting of a solid oral composition, a tablet, a capsule, a lozenge 3 and a dry powder for inhalation. 109 WO 2008/137809 PCT/US2008/062584 1 36. The pharmaceutical composition of claim 35 wherein the solid oral 2 composition is a tablet, capsule or lozenge. 1 37. The pharmaceutical composition of claim 33, wherein said 2 therapeutically effective amount is an amount effective to inhibit platelet aggregation in the 3 mammal. 1 38. The pharmaceutical composition of claim 37, wherein said platelet 2 aggregation is platelet ADP-dependent aggregation. 1 39. The pharmaceutical composition of claim 38, wherein said mammal is 2 a human. 1 40. The pharmaceutical composition of claim 33, wherein said compound 2 is an effective inhibitor of [ 3 H]2-MeS-ADP binding to platelet ADP receptors. 1 41. The pharmaceutical composition of claim 33, wherein the composition 2 is a solid oral composition. 1 42. The pharmaceutical composition of claim 33, wherein the composition 2 is a tablet, capsule or lozenge. 1 43. The pharmaceutical composition of claim 33, wherein the composition 2 is an aerosol or dry powder for inhalation. 1 44. The pharmaceutical composition of claim 33, wherein the composition 2 is in a form suitable for infusion, injection, or transdermal delivery. 1 45. A pharmaceutical composition comprising a therapeutically effective 2 amount of a compound according to claim 1 and an additional therapeutic agent. 1 46. The pharmaceutical composition according to claim 45, wherein the 2 additional therapeutic agent is useful for treating a condition or disorder selected from the 3 group consisting of thrombosis, acute myocardial infarction, unstable angina, chronic stable 4 angina, transient ischemic attacks, strokes, peripheral vascular disease, 5 preeclampsia/eclampsia, deep venous thrombosis, embolism, disseminated intravascular 6 coagulation and thrombotic cytopenic purpura, thrombotic and restenotic complications 110 WO 2008/137809 PCT/US2008/062584 7 following invasive procedures resulting from angioplasty, carotid endarterectorny, post 8 CABG (coronary artery bypass graft) surgery, vascular gram surgery, stent placements and 9 insertion of endovascular devices, prostheses, and hypercoagulable states related to genetic 10 predisposition or cancers. 1 47. A pharmaceutical composition for preventing or treating a condition in 2 a mammal characterized by undesired thrombosis comprising a pharmaceutically acceptable 3 carrier and a therapeutically effective amount of a salt of claim 1. 1 48. A method of preparing a salt of formula I: F / \ 0 01 C H ,C H N H N O O I N \ _/NH HN -/ 2 0 3 I. 4 comprising contacting a base with a compound of formula II: F 0N / 0 C1 NHN NH S HN / 0 0 5 0 6 II 7 or a salt thereof under conditions to form the salt of formula I. 1 49. The method of claim 48, wherein the conditions comprise performing 2 the method at a temperature of less than 10 'C. 1 50. The method of claim 48, wherein the salt of formula I is afforded in a 2 yield of at least 50%. 1 51. The method of claim 48, wherein the salt of formula I is afforded in a 2 yield of at least 65%. 1 52. The method of claim 48, wherein the salt of formula I is afforded in a 2 yield of at least 75%. 111 WO 2008/137809 PCT/US2008/062584 1 53. The method of claim 48, wherein the salt of formula I is prepared on a 2 gram scale or a kilogram scale. 1 54. A method for preventing or treating thrombosis and thrombosis related 2 conditions in a mammal comprising the step of administering to a mammal a therapeutically 3 effective amount of a salt of claim 1. 1 55. A method for preventing or treating a condition or disorder mediated at 2 least in part by ADP-induced platelet aggregation in a mammal comprising the step of 3 administering to a mammal in need of such treatment in a therapeutically effective amount of 4 a composition of claim 1 or a pharmaceutically acceptable salt thereof. 1 56. A method for inhibiting the coagulation of a blood sample comprising 2 the step of contacting said sample with said salt a salt of claim 1. 1 57. The method of claim 55, wherein said mammal is prone to or suffers 2 from a cardiovascular disease. 1 58. The method of claim 57, wherein said cardiovascular disease is at least 2 one selected from the group consisting of acute myocardial infarction, unstable angina, 3 chronic stable angina, transient ischemic attacks, strokes, peripheral vascular disease, 4 preeclampsia/eclampsia, deep venous thrombosis, embolism, disseminated intravascular 5 coagulation and thrombotic cytopenic purpura, thrombotic and retenotic complications 6 following invasive procedures resulting from angioplasty, carotid endarterectorny, post 7 CABG (coronary artery bypass graft) surgery, vascular gram surgery, stent, in-stent 8 thrombosis, and insertion of endovascular devices and prostheses, and hypercoagulable states 9 related to genetic predisposition or cancers. 1

59. The method of claim 54, wherein the compound is administered orally, 2 parenterally or topically 1 60. The method of claim 54, wherein the compound is administered in 2 combination with a second therapeutic agent. 1 61. The method of claim 60, wherein the patient is a human. 112 WO 2008/137809 PCT/US2008/062584 1

62. The method of claim 60, wherein the second therapeutic agent is useful 2 for treating a condition or disorder selected from the group consisting of acute myocardial 3 infarction, unstable angina, chronic stable angina, transient ischemic attacks, strokes, 4 peripheral vascular disease, preeclampsia/eclampsia, deep venous thrombosis, embolism, 5 disseminated intravascular coagulation and thrombotic cytopenic purpura, thrombotic and 6 restenotic complications following invasive procedures resulting from angioplasty, carotid 7 endarterectomy, post CABG (coronary artery bypass graft) surgery, vascular gram surgery, 8 stent placements and insertion of endovascular devices, prostheses, and hypercoagulable 9 states related to genetic predisposition and cancer. 1

63. The method in accordance with claim 60, wherein said compound is 2 administered in combination with a second therapeutic agent selected from the group 3 consisting of antiplatelet compounds, anticoagulants, fibrinolytics, anti-inflammatory 4 compounds, cholesterol-lowering agents, proton pump inhibitors, blood pressure-lowering 5 agents, serotonin blockers, and nitrates (i.e. nitroglycerin). 1

64. The method in accordance with claim 63, wherein said second 2 therapeutic agent is an antiplatelet compound selected from the group consisting of GPIIB 3 Ila antagonists, aspirin, phosphodiesterase III inhibitors and thromboxane A2 receptor 4 antagonists. 1

65. The method in accordance with claim 63, wherein said second 2 therapeutic agent is an anticoagulant selected from the group consisting of thrombin 3 inhibitors, coumadin, heparin and Lovenox@, and fXa inhibitors. 1

66. The method in accordance with claim 63, wherein said second 2 therapeutic agent is an anti-inflammatory compound selected from the group consisting of 3 non-steroidal anti-inflammatory agents, cyclooxygenase-2 inhibitors and rheumatoid arthritis 4 agents. 1

67. A method for preventing the occurrence of a secondary ischemic event 2 comprising administering to a patient who has suffered a primary ischemic event a 3 therapeutically effective amount of a salt of claim 1, together with a pharmaceutically 4 acceptable carrier. 113 WO 2008/137809 PCT/US2008/062584 1 68. The method in accordance with claim 67, wherein said primary and/or 2 secondary ischemic event is selected from the group consisting of myocardial infarction, 3 stable or unstable angina, acute re-occlusion after percutaneous coronary intervention, and/or 4 stenting, restenosis, peripheral vessel ballon angioplasty and/or stenting, thrombotic stroke, 5 transient ischemic attack, reversible ischemic neurological deficit and intermittent 6 claudication. 1 69. The method in accordance with claim 67, wherein said primary and/or 2 secondary ischemic event is selected from the group consisting of percutaneous coronary 3 intervention (PCI) including angioplasty and/or stent, acute myocardial infarction (AMI), 4 unstable angina (USA), coronary artery disease (CAD), transient ischemic attacks (TIA), 5 stroke, peripheral vascular disease (PVD), Surgeries-coronary bypass, carotid endarectomy. 1 70. A method for the preparation of a pharmaceutical composition 2 comprising admixing a therapeutically effective amount of the salt of claim 1 with a 3 pharmaceutically acceptable vehicle or carrier. 114