AU2007286451A1

AU2007286451A1 - A conjugate of an antibody against CCR5 and an antifusogenic peptide

Info

Publication number: AU2007286451A1
Application number: AU2007286451A
Authority: AU
Inventors: Michael Brandt; Stephan Fischer; Erhard Kopetzki; Suryanarayana Sankuratri; Ralf Schumacher
Original assignee: F Hoffmann La Roche AG
Current assignee: F Hoffmann La Roche AG
Priority date: 2006-08-17
Filing date: 2007-08-14
Publication date: 2008-02-21
Also published as: KR101105610B1; BRPI0715794A2; CA2658474A1; IL195667A0; JP2010500984A; KR20090037944A; WO2008019817A1; EP2054086A1; MX2009001204A

Description

WO 2008/019817 PCT/EP2007/007164 1 A conjugate of an antibody against CCR5 and an antifusogenic peptide The present invention relates to a conjugate of an antibody against CCR5 and an antifusogenic peptide wherein one to eight antifusogenic peptides are each conjugated to one terminus of the heavy and/or light chains of an anti-CCR5 antibody. The antifusogenic peptides can be different, similar or identical on the 5 amino acid level. Background of the Invention The infection of cells by the human immunodeficiency virus (HIV) is effected by a process in which the membrane of the cells to be infected and the viral membrane 10 are fused. A general scheme for this process is proposed: The viral envelope glycoprotein complex (gpl20/gp41) interacts with a cell surface receptor located on the membrane of the cell to be infected. The binding of gpl20 to, e.g., the CD4 receptor in combination with a co-receptor such as CCR-5 or CXCR-4 causes a change in the conformation of the gpl20/gp41 complex. In consequence of this 15 conformational change the gp41 protein is able to insert into the membrane of the target cell. This insertion is the beginning of the membrane fusion process. It is known that the amino acid sequence of the gp41 protein varies in different HIV strains because of naturally occurring polymorphisms. But the same domain architecture can be recognized, more precisely, a fusion signal, two heptad repeat 20 domains (HR1, HR2) and a transmembrane domain (in N- to C-terminal direction). It is suggested that the fusion (or fusogenic) domain is participating in the insertion into and the disintegration of the cell membrane. The HR regions are built up of multiple stretches comprising seven amino acids ("heptad") (see e.g. Shu, W., et al., Biochemistry 38 (1999) 5378-5385). Beside the heptads one or more 25 leucine zipper-like motifs are present. This composition accounts for the formation of a coiled coil structure of gp41 proteins and just as well of peptides derived from these domains. Coiled coils are in general oligomers consisting of two or more interacting helices. Peptides with amino acid sequences deduced from the HR1 or the HR2 domain of gp41 are effective in vitro and in vivo inhibitors of HIV uptake 30 into cells (for example peptides see e.g. US 5,464,933, US 5,656,480, US 6,258,782, US 6,348,568, or US 6,656,906). For example, T20 (also known as DP178, Fuzeon®, a HR2 peptide), T651 (US 6,479,055), and T2635 (WO 2004/029074) are very potent inhibitors of HIV infection. It has been attempted to enhance the efficacy of HR2 derived peptides with, for example, amino acid substitutions or chemical WO 2008/019817 PCT/EP2007/007164 -2 crosslinking (Sia, S.K., et al., Proc. Natl. Acad. Sci. USA 99 (2002) 14664-14669; Otaka, A., et al., Angew. Chem. Int. Ed. 41 (2002) 2937-2940). The conjugation of peptides to certain molecules can change their pharmacokinetic properties, e.g. the serum half-life of such peptide conjugates can be increased. 5 Conjugations are reported, for example, for polyethylene glycol (PEG) and Interleukin-6 (EP 0 442 724), for PEG and Erythropoietin (WO 01/02017), for chimeric molecules comprising Endostatin and immunoglobulins (US 2005/008649), for secreted antibody based fusion proteins (US 2002/147311), for fusion polypeptides comprising albumin (US 2005/0100991; human serum 10 albumin US 5,876,969), for PEGylated polypeptides (US 2005/0114037), and for interferon fusions. Also described in the state of the art are immunotoxins comprising Gelonin and an antibody (WO 94/26910), modified transferrin antibody fusion proteins (US 2003/0226155), antibody-cytokine fusion proteins (US 2003/0049227), and fusion proteins consisting of a peptide with immuno 15 stimulatory, membrane transport, or homophilic activity and an antibody (US 2003/0103984). In WO 2004/085505 long acting biologically active conjugates consisting of biologically active compounds chemically linked to macromolecules, are reported. The co-receptor CCR5 is used by most HIV-1 primary isolates and is critical for the 20 establishment and maintenance of infection. In addition, CCR5 function is dispensable for human health. A mutant CCR5 allele, "CCR5 A 32", encodes a truncated, non-functional protein (Samson, M., et al., Nature 382 (1996) 722-725; Dean, M., et al., Science 273 (1996) 1856-1862). Individuals homozygous for the mutation lack CCR5 expression and are strongly protected from HIV-1 infection. 25 They demonstrate no overt phenotype consequence and are highly resistant to M tropic HIV infection, whereas heterozygote individuals present delayed disease progression (Schwarz, M.K. and Wells, T.N., Nat. Rev. Drug Discov. 1 (2002) 347 358). The lack of CCR5 is without apparent adverse consequences, probably because CCR5 is part of a highly redundant chemokine network as receptor for the 30 cc-chemokines MIP-la, MIP-11, and RANTES, which share many overlapping functions, and most of which have alternative receptors (Rossi, D. and Zlotnik, A., Annu. Rev. Immunol. 18 (2000) 217-242). The identification of CCR5 as an HIV-1 co-receptor was based on the ability of its ligands, MIP-lx, MIP-11, and RANTES, to block infection by R5 but not RSX4 or X4 isolates (Cocchi, F., et al., Science 270 35 (1995) 1811-1815). CCR5 is also a receptor of the "cluster" chemokines, which are produced primarily during inflammatory responses and control the recruitment of WO 2008/019817 PCT/EP2007/007164 -3 neutrophils (CXC chemokines), macrophages and a subset of T cells (T helper Thl and Th2 cells). Thl responses are typically those involving cell-mediated immunity effective against viruses and tumors, proinflammatory responses responsible for killing intracellular parasites, and perpetuating autoimmune responses, for 5 example, whereas Th2 responses are believed to be pivotal in allergies. Therefore, inhibitors of these chemokine receptors may be useful as immunomodulators. For Thl responses, overactive responses are dampened, for example, in autoimmunity including rheumatoid arthritis, or, for Th2 responses, asthma attacks or allergic responses including atopic dermatitis are lessened (see e.g. Schols, D., Curr. Top. 10 Med. Chem. 4 (2004) 883-893; Mueller, A., and Strange, P.G., Int. J. Biochem. Cell Biol. 36 (2004) 35-38; Kazmierski, W.M., et al., Curr. Drug Targets Infect. Disord. 2 (2002) 265-278; Lehner, T., Trends Immunol. 23 (2002) 347-351). Antibodies against human CCR5 are e.g. PRO 140 (Olson, W.C., et al., J. Virol. 73 (1999) 4145-4155), and/or 2D7 (Samson, M., et al., J. Biol. Chem. 272 (1997) 15 24934-24941). Additional antibodies are mentioned in US 2004/0043033, US 6,610,834, US 2003/0228306, US 2003/0195348, US 2003/0166870, US 2003/0166024, US 2003/0165988, US 2003/0152913, US 2003/0100058, US 2003/0099645, US 2003/0049251, US 2003/0044411, US 2003/0003440, US 6,528,625, US 2002/0147147, US 2002/0146415, US 2002/0106374, 20 US 2002/0061834, US 2002/0048786, US 2001/0000241, EP 1 322 332, EP 1 263 791, EP 1 207 202, EP 1 161 456, EP 1 144 006, WO 2003/072766, WO 2003/066830, WO 2003/033666, WO 2002/083172, WO 02/22077, WO 01/58916, WO 01/58915, WO 01/43779, WO 01/42308, and EP 05007138.0. Polyethylene glycol conjugates of antibodies against CCR5 are known from 25 US 2003/0228306. US 2003/0215421 refers to chemokine-toxin conjugates. WO 01/43779 refers to conjugates of anti-CD4 antibodies and anti-CCR5 antibodies and to conjugates of anti-CD4 antibodies and an HIV-1 fusion inhibiting peptide. Conjugates of CCR5 antibodies and toxins are mentioned in EP 1 346 731. 30 Summary of the Invention The current invention reports a conjugate comprising one to eight antifusogenic peptides and an antibody against an HIV gpl20 binding cell surface receptor, characterized in that one to eight, preferably two or four, antifusogenic peptides 35 each conjugate to one terminus of the heavy and/or light chains of said antibody WO 2008/019817 PCT/EP2007/007164 -4 against an HIV gpl20 binding cell surface receptor (a number of eight antifusogenic peptides per antibody is only possible if the antibody comprises eight termini, i.e. is composed e.g. of two heavy chains and two light chains; if the antibody comprises a smaller number of C- and N-termini, e.g. as a scFv, the 5 corresponding number of antifusogenic peptides possible at maximum in the conjugate is also reduced, i.e. it is reduced to less than eight). The invention further comprises a conjugate comprising one or more antifusogenic peptides and an anti-CCR5 antibody (mAb CCR5) characterized in that at most one to eight antifusogenic peptides are each conjugated to one terminus of the 10 heavy and/or light chains of said anti-CCR5 antibody (a number of eight antifusogenic peptides per mAb CCR5 is only possible if the mAb CCR5 comprises eight termini, i.e. is composed e.g. of two heavy chains and two light chains; if the mAb CCR5 comprises a smaller number of C- and N-termini, e.g. as a scFv, the corresponding number of antifusogenic peptides possible at maximum in the 15 conjugate is also reduced, i.e. it is reduced to less than eight). Preferably the carboxy-terminal amino acid of an anti-CCR5 antibody chain is conjugated to the amino-terminal amino acid of the antifusogenic peptide or the carboxy-terminal amino acid of the antifusogenic peptide is conjugated to the amino-terminal amino acid of the antibody chain, preferably by a peptide bond 20 with or without an intermediate linker. Preferably the conjugate is characterized by the general formula mAb CCR5 - [linker]m - [antifusogenic peptide]n wherein m is independently for each antifusogenic peptide either 0 (i.e. a peptide bond between mAb CCR5 and antifusogenic peptide) or 1 (i.e. a linker between 25 mAb CCR5 and antifusogenic peptide) and n is an integer of from 1 to 8. A preferred conjugate of a heavy and/or light chain of mAb CCR5 and an antifusogenic peptide ("chain conjugate") is selected from the group consisting of: (1) [antifusogenic peptide] - [linker]m - [heavy chain] (2) [heavy chain] - [linker]m - [antifusogenic peptide] 30 (3) [antifusogenic peptide] - [linker]m - [heavy chain] - [antifusogenic peptide] (4) [antifusogenic peptide] - [linker]m - [light chain] (5) [light chain] - [linker]m - [antifusogenic peptide] WO 2008/019817 PCT/EP2007/007164 -5 (6) [antifusogenic peptide] - [linker]m - [light chain] - [antifusogenic peptide] (7) [antifusogenic peptide] - [linker]m - [heavy chain] - [linker]m [antifusogenic peptide] 5 (8) [antifusogenic peptide] - [linker]m - [light chain] - [linker]m [antifusogenic peptide] wherein the linker can be the same or different in (within and between) said chain conjugates, wherein m is an integer of 1 or 0, and m can be independently the same or different in (within and between) said chain conjugates. 10 (Left side of the peptide or mAb CCR5 chain means N-terminus, right side means C-terminus. In (1) therefore the C-terminus of the antifusogenic peptide is linked by a peptide bond or a linker to the N-terminus of the heavy chain of mAb CCR5). Preferably the chain conjugates are assembled to conjugates according to the invention comprising a mAb CCR5 (e.g. consisting of two light chains and two 15 heavy chains including the constant Fc domains, a scFv fragment, or a Fab fragment). Especially preferred chain conjugates are (2), (3), (4), and (7). Especially preferred conjugates according to the invention comprise 2x [mAb CCR5 light chain] and 2x (2), 2 x [mAb CCR5 light chain] and 2 x (3), or 2 x [mAb CCR5 heavy chain] and 2 20 x (4), or 2 x [mAb CCR5 light chain] and 2 x (7). The heavy and/or light chain comprises preferably a constant region (Fc). Preferably the conjugate is characterized in comprising a variable heavy chain domain consisting of an immunoglobulin framework and a CDR3 region selected from the group consisting of the heavy chain CDR3 sequences SEQ ID NO: 16, 17. 25 Preferably the conjugate is characterized in comprising a variable heavy chain domain consisting of an immunoglobulin framework and a CDR3 region selected from the group consisting of CDR3 sequences SEQ ID NO: 16, 17, a CDR2 region selected from the group consisting of CDR2 sequences SEQ ID NO: 13, 14, 15, and a CDR1 region selected from the group consisting of CDR1 sequences SEQ ID NO: 30 9, 10, 11, 12. Preferably the conjugate is characterized in comprising a heavy chain variable domain selected from the group of heavy chain variable domains comprising SEQ ID NO: 1, 3, 5, and 7.

WO 2008/019817 PCT/EP2007/007164 -6 Preferably the conjugate is characterized in comprising a variable light chain domain consisting of an immunoglobulin framework and a CDR1 region selected from SEQ ID NO:18, 19, 20, a CDR2 region selected from SEQ ID NO:21, 22, 23, and a CDR3 region selected from SEQ ID NO:24, 25. 5 Preferably the conjugate is characterized in comprising as heavy chain CDRs the CDRs of SEQ ID NO: 1 and as light chain CDRs the CDRs of SEQ ID NO: 2, as heavy chain CDRs the CDRs of SEQ ID NO: 3 and as light chain CDRs the CDRs of SEQ ID NO: 4, as heavy chain CDRs the CDRs of SEQ ID NO: 5 and as light chain CDRs the CDRs of SEQ ID NO: 6, or as heavy chain CDRs the CDRs of SEQ ID 10 NO: 7 and as light chain CDRs the CDRs of SEQ ID NO: 8. Preferably the conjugate is characterized in comprising a variable heavy and light chain domain independently selected from the group consisting of a) the heavy chain (VH) variable domain defined by amino acid sequence SEQ ID NO: 1 and the light chain (VL) variable domain defined by amino acid 15 sequence SEQ ID NO: 2; b) the heavy chain variable domain defined by amino acid sequence SEQ ID NO: 3 and the light chain variable domain defined by amino acid sequence SEQ ID NO: 4; c) the heavy chain variable domain defined by amino acid sequence SEQ ID NO: 20 5 and the light chain variable domain defined by amino acid sequence SEQ ID NO: 6; d) the heavy chain variable domain defined by amino acid sequence SEQ ID NO: 7 and the light chain variable domain defined by amino acid sequence SEQ ID NO: 8. 25 Preferably the conjugate is characterized in comprising the heavy chain (VH) variable domain defined by amino acid sequence SEQ ID NO: 1 and the light chain (VL) variable domain defined by amino acid sequence SEQ ID NO:2; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:3 and the light 30 chain variable domain defined by amino acid sequence SEQ ID NO:4; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:5 and the light chain variable domain defined by amino acid sequence SEQ ID NO:6; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:7 and the light chain variable domain defined by amino acid sequence SEQ ID NO:8; a linker 35 selected from the group consisting of the amino acids glycine (G) and asparagine (N), the tripeptide GST, and SEQ ID NO:36-62 and SEQ ID NO:67-70; and an WO 2008/019817 PCT/EP2007/007164 -7 antifusogenic peptide selected from the group of peptides defined by SEQ ID NO:29 to 35 and SEQ ID NO: 73. Preferably the conjugate is characterized in comprising an antifusogenic peptide selected from the group of peptides comprising C34, T20, T1249, T651, T2635, 5 N36, DP107, and afp-1. Preferably the conjugate is characterized in comprising an antifusogenic peptide at each C-terminus of the heavy chains or at each N-terminus of the light chains (two antifusogenic peptides). Preferably the conjugate is characterized in that it comprises an antifusogenic peptide at each C-terminus of the heavy chains and at 10 each N-terminus of the light chains (four antifusogenic peptides). Preferably the conjugate is characterized in comprising two light chain variable domains of SEQ ID NO:2, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:l, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, in comprising two light chain variable 15 domains of SEQ ID NO:4, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:3, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, in comprising two light chain variable domains of SEQ ID NO:6, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:5, a linker of SEQ ID NO:40 and an 20 antifusogenic peptide of SEQ ID NO:33, or in comprising two light chain variable domains of SEQ ID NO:8, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:7, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33. Preferably the conjugate is characterized in that said anti-CCR5 antibody is of IgG1 25 subclass. It is also preferred, that said anti-CCR5 antibody is of IgG4 subclass, or of IgG1 or IgG2 subclass, with a mutation in amino acid position S228, L234, L235, and/or D265, and/or contains the PVA236 mutation. Preferably the conjugate is characterized in that said anti-CCR5 antibody of IgG4 subclass has a S228P mutation and said anti-CCR5 antibody of IgG1 subclass has L234A and L235A 30 mutations. The invention further comprises a conjugate comprising two or more antifusogenic peptides and an anti-CD4 antibody (mAb CD4) characterized in that two to eight antifusogenic peptides, preferably two or four, are each conjugated to one terminus of the heavy and/or light chains of said anti-CD4 antibody (a number of eight WO 2008/019817 PCT/EP2007/007164 -8 antifusogenic peptides per mAb CD4 is only possible if the mAb CD4 comprises eight termini, i.e. is composed e.g. of two heavy chains and two light chains; if the mAb CD4 comprises a smaller number of C- and N-termini, e.g. as a scFv, the corresponding number of antifusogenic peptides possible at maximum in the 5 conjugate is also reduced, i.e. it is reduced to less than eight). Preferably the carboxy-terminal amino acid of an antibody chain is conjugated to the amino-terminal amino acid of the antifusogenic peptide or the carboxy terminal amino acid of the antifusogenic peptide is conjugated to the amino terminal amino acid of the antibody chain, preferably by a peptide bond with or 10 without an intermediate linker. Preferably the conjugate is characterized by the general formula antibody - [linker]m - [antifusogenic peptide]n wherein m is independently for each antifusogenic peptide either 0 (i.e. a peptide bond between the antibody and antifusogenic peptide) or 1 (i.e. a linker between 15 the antibody and antifusogenic peptide) and n is an integer of from 2 to 8, preferably an even integer of from 2 to 8, more preferably 2 or 4. Preferably said antibody is mAb CD4. A preferred conjugate of a heavy and/or light chain of an antibody and an antifusogenic peptide ("chain conjugate") is selected from the group consisting of 20 (in N-terminal to C-terminal direction): (1) [antifusogenic peptide] - [linker]m - [heavy chain] (2) [heavy chain] - [linker]m - [antifusogenic peptide] (3) [antifusogenic peptide] - [linker]m - [heavy chain] - [antifusogenic peptide] 25 (4) [antifusogenic peptide] - [linker]m - [light chain] (5) [light chain] - [linker]m - [antifusogenic peptide] (6) [antifusogenic peptide] - [linker]m - [light chain] - [antifusogenic peptide] (7) [antifusogenic peptide] - [linker]m - [heavy chain] - [linker]m 30 [antifusogenic peptide] (8) [antifusogenic peptide] - [linker]m - [light chain] - [linker]m [antifusogenic peptide] WO 2008/019817 PCT/EP2007/007164 -9 wherein the linker can be the same or different in (within and between) said chain conjugates, wherein m is an integer of 1 or 0, and m can be independently the same or different in (within and between) said chain conjugates. (Left side of the peptide or antibody chain means N-terminus, right side means C 5 terminus. In (1) therefore the C-terminus of the antifusogenic peptide is linked by a peptide bond or a linker to the N-terminus of the heavy chain of the antibody). Preferably the chain conjugates are assembled to conjugates according to the invention comprising a mAb CD4 (e.g. consisting of two light chains and two heavy chains including the constant Fc domains, a scFv fragment, or a Fab fragment). 10 Especially preferred chain conjugates are (2), (3), (4), and (7). Especially preferred conjugates according to the invention comprise 2x [mAb CD4 light chain] and 2x (2), 2 x [mAb CD4 light chain] and 2 x (3), or 2 x [mAb CD4 heavy chain] and 2 x (4), or 2 x [mAb CD4 light chain] and 2 x (7). The heavy and/or light chain comprises preferably a constant region. 15 Preferably the conjugate is characterized in comprising a variable heavy chain domain consisting of an immunoglobulin framework and a CDR3 region selected from the heavy chain CDR3 sequences of SEQ ID NO:103, 104, or 105. Preferably the conjugate is characterized in comprising a variable heavy chain domain consisting of an immunoglobulin framework and a CDR3 region selected 20 from the CDR3 sequences of SEQ ID NO:103, 104, or 105, a CDR2 region selected from the CDR2 sequences of SEQ ID NO:100, 101, or 102, and a CDR1 region selected from the CDR1 sequences of SEQ ID NO:97, 98, or 99. Preferably the conjugate is characterized in comprising a heavy chain variable domain wherein the heavy chain variable domain comprises SEQ ID NO:82, or 83. 25 Preferably the conjugate is characterized in comprising a variable light chain domain consisting of an immunoglobulin framework and a CDR1 region selected from SEQ ID NO:87, 88, 89, or 90, a CDR2 region selected from SEQ ID NO:91, 92, or 93, and a CDR3 region selected from SEQ ID NO:94, 95, or 96. Preferably the conjugate is characterized in comprising as heavy chain CDRs the 30 CDRs of SEQ ID NO:81, and as light chain CDRs the CDRs of SEQ ID NO:75, as heavy chain CDRs the CDRs of SEQ ID NO:82, and as light chain CDRs the CDRs of SEQ ID NO:76, as heavy chain CDRs the CDRs of SEQ ID NO:84, and as light WO 2008/019817 PCT/EP2007/007164 - 10 chain CDRs the CDRs of SEQ ID NO:78, as heavy chain CDRs the CDRs of SEQ ID NO:85, and as light chain CDRs the CDRs of SEQ ID NO:79, or as heavy chain CDRs the CDRs of SEQ ID NO:86, and as light chain CDRs the CDRs of SEQ ID NO:80. 5 Preferably the conjugate is characterized in comprising a variable heavy and light chain domain independently selected from: a) the heavy chain variable domain defined by amino acid sequence SEQ ID NO:81, and the light chain variable domain defined by amino acid sequence SEQ ID NO:75; 10 b) the heavy chain variable domain defined by amino acid sequence SEQ ID NO:82, and the light chain variable domain defined by amino acid sequence SEQ ID NO:76; c) the heavy chain variable domain defined by amino acid sequence SEQ ID NO:84, and the light chain variable domain defined by amino acid sequence 15 SEQ ID NO:78; d) the heavy chain variable domain defined by amino acid sequence SEQ ID NO:85, and the light chain variable domain defined by amino acid sequence SEQ ID NO:79, e) the heavy chain variable domain defined by amino acid sequence SEQ ID 20 NO:86, and the light chain variable domain defined by amino acid sequence SEQ ID NO:80. Preferably the conjugate is characterized in comprising the heavy chain variable domain defined by amino acid sequence SEQ ID NO:81, and the light chain 25 variable domain defined by amino acid sequence SEQ ID NO:75; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:82, and the light chain variable domain defined by amino acid sequence SEQ ID NO:76; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:84, and the light chain variable domain defined by amino acid sequence SEQ ID NO:78; the 30 heavy chain variable domain defined by amino acid sequence SEQ ID NO:85, and the light chain variable domain defined by amino acid sequence SEQ ID NO:79, or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:86, and the light chain variable domain defined by amino acid sequence SEQ ID NO:80; a linker selected from the amino acids glycine (G) and asparagine (N), the 35 tripeptide GST, and the SEQ ID NO:36 to 62 and SEQ ID NO:67 to 70; and an WO 2008/019817 PCT/EP2007/007164 -11 antifusogenic peptide selected from the antifusogenic peptides of SEQ ID NO:29 to 35 or SEQ ID NO:73. Preferably the conjugate is characterized in comprising an antifusogenic peptide selected from the antifusogenic peptides C34, T20, T1249, T651, T2635, N36, 5 DP107, or afp-1. Preferably the conjugate is characterized in comprising an antifusogenic peptide at each C-terminus of the heavy chains or at each N-terminus of the light chains (two antifusogenic peptides). Preferably the conjugate is characterized in that it comprises an antifusogenic peptide at each C-terminus of the heavy chains and at 10 each N-terminus of the light chains (four antifusogenic peptides). Preferably the conjugate is characterized in that it comprises an antifusogenic peptide at the two C-termini of the heavy chains and at the two N-termini of the light chains (four antifusogenic peptides) Preferably the conjugate is characterized in comprising two light chain variable 15 domains of SEQ ID NO:75, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:81, a linker of SEQ ID NO:69, and an antifusogenic peptide of SEQ ID NO:73, in comprising two light chain variable domains of SEQ ID NO:76, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:82, a linker of SEQ ID NO:69, and an 20 antifusogenic peptide of SEQ ID NO:73, in comprising two light chain variable domains of SEQ ID NO:78, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:84, a linker of SEQ ID NO:69, and an antifusogenic peptide of SEQ ID NO:73, in comprising two light chain variable domains of SEQ ID NO:79, two conjugates of type (2) each comprising a heavy 25 chain variable domain of SEQ ID NO:85, a linker of SEQ ID NO:69, and an antifusogenic peptide of SEQ ID NO:73, or in comprising two light chain variable domains of SEQ ID NO:80, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:86, a linker of SEQ ID NO:69, and an antifusogenic peptide of SEQ ID NO:73. 30 Preferably the conjugate is characterized in that said antibody is of IgG1 subclass or of IgG4 subclass. It is also preferred, that said antibody is of IgG4 or IgG1 or IgG2 subclass, with a mutation in amino acid position S228, L234, L235, and/or D265, and/or contains the PVA236 mutation. Preferably the conjugate is characterized in WO 2008/019817 PCT/EP2007/007164 - 12 that said antibody of IgG4 subclass has a S228P mutation and said antibody of IgG1 subclass has L234A and L235A mutations. Especially preferred is that said antibody is an anti-CD4 antibody or an anti-CCR5 antibody. 5 The invention comprises a method for the production of a conjugate according to the invention, characterized in that the method comprises a) cultivating a cell containing one or more plasmids containing one or more nucleic acid molecules encoding a conjugate according to the invention under conditions suitable for the expression of the conjugate, 10 b) recovering the conjugate from the cell or the supernatant. In one embodiment are the genes encoding the light and heavy chains of mAb CCR5 or mAb CD4 with or without linked antifusogenic peptide located on the same expression vector or on different expression vectors. 15 The invention comprises a pharmaceutical composition, containing a conjugate according to the invention, together with a pharmaceutically acceptable excipient or carrier. The invention comprises the use of a conjugate according to the invention for the manufacture of a medicament for the treatment of viral infections. Preferably the 20 use is characterized in that the viral infection is a HIV infection. The invention comprises the use of a conjugate according to the invention for the treatment of a patient in need of an antiviral treatment, preferably an anti HIV treatment. Description of the Invention 25 One aspect of the current invention is a conjugate comprising one or more antifusogenic peptides and an anti-CCR5 antibody (mAb CCR5) characterized in that one to eight antifusogenic peptides are each conjugated to one terminus of the heavy and/or light chains of said anti-CCR5 antibody. A number of eight 30 antifusogenic peptides per mAb CCR5 is only possible if the mAb CCR5 comprises eight termini, i.e. is composed e.g. of two heavy chains and two light chains. If the mAb CCR5 comprises a smaller number of C- and N-termini, e.g. as a scFv, the corresponding number of antifusogenic peptides possible at maximum in the WO 2008/019817 PCT/EP2007/007164 - 13 conjugate is also reduced, i.e. it is reduced to less than eight. Another aspect of the current invention is conjugate comprising two or more antifusogenic peptides and an anti-CD4 antibody (mAb CD4) characterized in that two to eight antifusogenic peptides are each conjugated to one terminus of the heavy and/or light chains of 5 said anti-CD4 antibody. When in the following reference is made to antibody in general or to mnAb CCR5 or to mAb CD4 it is also included where appropriate the same reference to the other mAb and the corresponding sequences. An "antifusogenic peptide" is a peptide which inhibits events associated with 10 membrane fusion or the membrane fusion event itself, including, among other things, the inhibition of infection of uninfected cells by a virus due to membrane fusion. These antifusogenic peptides are preferably linear peptides. For example, they can be derived from the gp41 ectodomain, e.g. such as DP107, DP178. Examples of such peptides can be found in US 5,464,933, US 5,656,480, 15 US 6,013,263, US 6,017,536, US 6,020,459, US 6,093,794, US 6,060,065, US 6,258,782, US 6,348,568, US 6,479,055, US 6,656,906, WO 1996/19495, WO 1996/40191, WO 1999/59615, WO 2000/69902, and WO 2005/067960. For example, the amino acid sequences of such peptides comprise or can be selected from the group of SEQ ID NO: 1 to 10 of US 5,464,933; SEQ ID NO: 1 to 15 of 20 US 5,656,480; SEQ ID NO: 1 to 10 and 16 to 83 of US 6,013,263; SEQ ID NO: 1 to 10, 20 to 83 and 139 to 149 of US 6,017,536; SEQ ID NO: 1 to 10, 17 to 83 and 210 to 214 of US 6,093,794; SEQ ID NO: 1 to 10, 16 to 83 and 210 to 211 of US 6,060,065; SEQ ID NO: 1286 and 1310 of US 6,258,782; SEQ ID NO: 1129, 1278-1309, 1311 and 1433 of US 6,348,568; SEQ ID NO: 1 to 10 and 210 to 238 of 25 US 6,479,055; SEQ ID NO: 1 to 171, 173 to 216, 218 to 219, 222 to 228, 231, 233 to 366, 372 to 398, 400 to 456, 458 to 498, 500 to 570, 572 to 620, 622 to 651, 653 to 736, 739 to 785, 787 to 811, 813 to 823, 825, 827 to 863, 865 to 875, 877 to 883, 885, 887 to 890, 892 to 981, 986 to 999, 1001 to 1003, 1006 to 1018, 1022 to 1024, 1026 to 1028, 1030 to 1032, 1037 to 1076, 1078 to 1079, 1082 to 1117, 1120 to 1176, 1179 30 to 1213, 1218 to 1223, 1227 to 1237, 1244 to 1245, 1256 to 1268, 1271 to 1275, 1277, 1345 to 1348, 1350 to 1362, 1364, 1366, 1368, 1370, 1372, 1374 to 1376, 1378 to 1379, 1381 to 1385, 1412 to 1417, 1421 to 1426, 1428 to 1430, 1432, 1439 to 1542, 1670 to 1682, 1684 to 1709, 1712 to 1719, 1721 to 1753, 1755 to 1757 of US 6,656,906; or SEQ ID NO: 5 to 95 of WO 2005/067960. The antifusogenic 35 peptide has an amino acid sequence comprising of from 5 to 100 amino acids, preferably of from 10 to 75 amino acids and more preferred of from 15 to 50 amino WO 2008/019817 PCT/EP2007/007164 - 14 acids. Especially preferred antifusogenic peptides are C-34, T-20, T-1249, T-651, T 2635, N-36, (Root, M.J., et al., Curr. Pharm. Des. 10 (2004) 1805-1825) and DP-107 (Wild, C., et al., Proc. Natl. Acad. Sci. USA 91 (1994) 12676-12680) and afp-1 (SEQ ID NO:29 to 35 and SEQ ID NO:73). In one embodiment comprises the conjugate 5 according to the invention one or more antifusogenic peptides and an antibody wherein i) said antifusogenic peptides are linear peptides with an amino acid sequence of from 5 to 100 amino acids, and ii) one to eight antifusogenic peptides are each conjugated to one terminus of the heavy and/or light chains of said antibody. In another embodiment comprises the conjugate according to the 10 invention one or more antifusogenic peptides and an antibody, e.g. an anti-CCR5 antibody (mAb CCR5), wherein i) said antifusogenic peptides are derived from the gp41 ectodomain, and ii) one to eight antifusogenic peptides are each conjugated to one terminus of the heavy and/or light chains of said antibody. The term "gp41 ectodomain" denotes the amino acid sequence starting with amino acid position 15 561 and ending with amino acid position 620 of HIV-1 gpl60 or starting with amino acid position 50 and ending with amino acid position 109 of HIV-1 gp41 (SEQ ID NO: 66) (see also e.g. Bar, S. and Alizon, M. J. Virol. 78 (2004) 811-820). The term "antibody" encompasses the various forms of antibody structures including whole antibodies and antibody fragments. The antibody according to the 20 invention is preferably a human antibody, a humanized antibody, a chimeric antibody, a T cell antigen depleted antibody (WO 98/33523, WO 98/52976, and WO 00/34317). Genetic engineering of antibodies is e.g. described in Morrison, S.L., et al., Proc. Natl. Acad Sci. USA 81 (1984) 6851-6855; US Patent Nos. 5,202,238 and 5,204,244; Riechmann, L., et al., Nature 332 (1988) 323-327; 25 Neuberger, M.S., et al., Nature 314 (1985) 268-270; Lonberg, N., Nat. Biotechnol. 23 (2005) 1117-1125. "Antibody fragments" comprise a portion of a full length antibody, preferably the variable domains thereof or at least the antigen binding portion thereof. Examples of antibody fragments are e.g. single-chain antibody molecules (scFv), Fab, F(ab) 2 30 fragments, and the like as long as they retain the characteristics of an anti-CCR5 antibody. ScFv antibodies are, e.g., described in Huston, J.S., Methods in Enzymol. 203 (1991) 46-88. Huston also describes linkers and methods for linking of polypeptides useful for the present invention. "CCR5" means human CCR5 as described, e.g., in Oppermann, M., Cell Signal. 16 35 (2004) 1201-1210 and SwissProt P51681. The terms "antibody binding to CCR5", WO 2008/019817 PCT/EP2007/007164 - 15 "anti-CCR5 antibody", or "mAb CCR5", which are used interchangeably within this application, mean an antibody specifically binding to CCR5 and preferably inhibiting HIV fusion with a target cell. Binding can be tested in a cell based in vitro ELISA assay (CCR5 expressing CHO cells). Binding is found if the antibody causes 5 an S/N (signal/noise) ratio of 5 or more, preferably 10 or more at an antibody concentration of 100 ng/ml. The term "inhibiting HIV fusion with a target cell" refers to inhibiting HIV fusion with a target cell measured in an assay comprising contacting said target cell (e.g. PBMC) with the virus in the presence of the antibody in a concentration effective to inhibit membrane fusion between the virus 10 and said cell and measuring e.g. luciferase reporter gene activity or the HIV p24 antigen concentration. The term "membrane fusion" refers to fusion between a first cell coexpressing CCR5 and CD4 polypeptides and a second cell or virus expressing an HIV env protein. Membrane fusion is determined by genetically engineered cells and/or viruses by a reporter gene assay (e.g. by luciferase reporter gene assay). 15 Preferred anti-CCR5 antibodies are mentioned in US 2004/0043033, US 6,610,834, US 2003/0228306, US 2003/0195348, US 2003/0166870, US 2003/0166024, US 2003/0165988, US 2003/0152913, US 2003/0100058, US 2003/0099645, US 2003/0049251, US 2003/0044411, US 2003/0003440, US 6,528,625, US 2002/0147147, US 2002/0146415, US 2002/0106374, US 2002/0061834, 20 US 2002/0048786, US 2001/0000241, EP 1 322 332, EP 1 263 791, EP 1 207 202, EP 1 161 456, EP 1 144 006, WO 2003/072766, WO 2003/066830, WO 2003/033666, WO 2002/083172, WO 02/22077, WO 01/58916, WO 01/58915, WO 01/43779, WO 01/42308, and WO 2006/103100. Especially preferred anti CCR5 antibodies are described in WO 2006/103100. An especially preferred anti 25 CCR5 antibody is characterized in that the antibody comprises a variable heavy chain domain consisting of an immunoglobulin framework and a CDR3 region selected from the group consisting of the heavy chain CDR3 sequences SEQ ID NO: 16, 17. A further preferred antibody comprises a variable heavy chain region consisting of an immunoglobulin framework and a CDR3 region selected from the 30 group consisting of CDR3 sequences SEQ ID NO: 16, 17, a CDR2 region selected from the group consisting of CDR2 sequences SEQ ID NO: 13, 14, 15, and a CDR1 region selected from the group consisting of CDR1 sequences SEQ ID NO: 9, 10, 11, 12. Preferred heavy chain variable domains are shown in SEQ ID NO: 1, 3, 5, 7. A preferred anti-CCR5 antibody comprises in addition a variable light chain 35 domain consisting of an immunoglobulin framework and a CDR1 region selected from the group consisting of CDR1 sequences SEQ ID NO:18, 19, 20, a CDR2 WO 2008/019817 PCT/EP2007/007164 - 16 region selected from the group consisting of CDR2 sequences SEQ ID NO:21, 22, 23, and a CDR3 region selected from the group of CDR3 sequences SEQ ID NO:24, 25. The anti-CCR5 antibody is preferably characterized in containing as heavy chain CDRs the CDRs of SEQ ID NO: 1 and as light chain CDRs the CDRs of SEQ 5 ID NO: 2, as heavy chain CDRs the CDRs of SEQ ID NO: 3 and as light chain CDRs the CDRs of SEQ ID NO: 4, as heavy chain CDRs the CDRs of SEQ ID NO: 5 and as light chain CDRs the CDRs of SEQ ID NO: 6, or as heavy chain CDRs the CDRs of SEQ ID NO: 7 and as light chain CDRs the CDRs of SEQ ID NO: 8. CDR sequences can be determined according to the standard definition of Kabat, 10 E.A., et al., Sequences of Proteins of Immunological Interest, 5th ed., Public Health Service, National Institutes of Health, Bethesda, MD (1991). CDRs of SEQ ID NO: 1-8 are shown in SEQ ID NO: 9-25. The anti-CCR5 antibody comprises preferably a variable heavy and light chain domain independently selected from the group consisting of 15 a) the heavy chain (VH) variable domain defined by amino acid sequence SEQ ID NO: 1 and the light chain (VL) variable domain defined by SEQ ID NO: 2; b) the heavy chain variable domain defined by amino acid sequence SEQ ID NO: 3 and the light chain variable domain defined by SEQ ID NO: 4; c) the heavy chain variable domain defined by amino acid sequence SEQ ID NO: 20 5 and the light chain variable domain defined by SEQ ID NO: 6; d) the heavy chain variable domain defined by amino acid sequence SEQ ID NO: 7 and the light chain variable domain defined by SEQ ID NO: 8. "CD4" means human CD4 as described, e.g., in Brady, R.L. and Barclay, A.N., Curr. 25 Top. Microbiol. Immunol. 205 (1996) 1-18 and SwissProt P01730. The terms "antibody binding to CD4", "anti-CD4 antibody", or "mAb CD4", which are used interchangeably within this application, denote an antibody specifically binding to CD4 and preferably inhibiting HIV fusion with a target cell. Binding can be tested in a cell based in vitro ELISA assay (CD4 expressing CHO cells). Binding is found if 30 the antibody in question causes an S/N (signal/noise) ratio of 5 or more, preferably 10 or more at an antibody concentration of 100 ng/ml. The term "inhibiting HIV fusion with a target cell" refers to inhibiting HIV fusion with a target cell measured in an assay comprising contacting said target cell (e.g. PBMC) with the virus in the presence of the antibody in question in a concentration effective to inhibit 35 membrane fusion between the virus and said cell and measuring, e.g., luciferase reporter gene activity or the HIV p24 antigen concentration. The term "membrane WO 2008/019817 PCT/EP2007/007164 - 17 fusion" refers to fusion between a first cell expressing CD4 polypeptides and a second cell or virus expressing an HIV env protein. Membrane fusion is determined by genetically engineered cells and/or viruses by a reporter gene assay (e.g. by luciferase reporter gene assay). Exemplary anti-CD4 antibodies are mentioned in 5 e.g. Reimann, K.A., et al., Aids Res. Human Retrovir. 13 (1997) 933-943, EP 0 512 112, US 5,871,732, EP 0 840 618, EP 0 854 885, EP 1 266 965, US 2006/0051346, WO 97/46697, WO 01/43779, US 6,136,310, WO 91/009966. "CXCR4" means human CXCR4 as described, e.g. in Feng, Y., et al., Science 272 (1996) 809-810, or Tamamura, H. and Fujii, N., Expert Opinion on Therapeutic 10 Targets 9 (2005) 1267-1282. The terms "antibody binding to CXCR4", "anti CXCR4 antibody", or "mAb CXCR4", which are used interchangeably within this application, denote an antibody specifically binding to CXCR4 and preferably inhibiting HIV fusion with a target cell. Binding can be tested in a cell based in vitro ELISA assay (CXCR4 expressing CHO cells). Binding is found if the antibody in 15 question causes an S/N (signal/noise) ratio of 5 or more, preferably 10 or more at an antibody concentration of 100 ng/ml. The term "inhibiting HIV fusion with a target cell" refers to inhibiting HIV fusion with a target cell measured in an assay comprising contacting said target cell (e.g. PBMC) with the virus in the presence of the antibody in question in a concentration effective to inhibit membrane fusion 20 between the virus and said cell and measuring, e.g., luciferase reporter gene activity or the HIV p24 antigen concentration. The term "membrane fusion" refers to fusion between a first cell expressing CXCR4 polypeptides and a second cell or virus expressing an HIV env protein. Membrane fusion is determined by genetically engineered cells and/or viruses by a reporter gene assay (e.g. by luciferase reporter 25 gene assay). Exemplary anti-CXCR4 antibodies are reported in Strizki, J.M., et al., J. Virol. 71 (1997) 5678-5683 (Ab 12G5), US 7,138,496, An "antibody against an HIV gpl20 binding cell surface receptor" denotes within the current invention an antibody that is binding to a cell surface receptor, which is used by the HIV gpl20 subunit to mediate the binding of the virus to the cell 30 surface of a cell, and thus prevents the binding of gpl20 to said receptor and also the binding of HIV to a cell surface. The HIV gpl20 is derived from the proteolytic cleavage of the precursor HIV gpl60 (see e.g. Brenneman, D.E., et al., Int. Rev. Neurobiol. 32 (1990) 305-353). The second fragment of this cleavage is the HIV gp41. These two fragments associate non-covalently and mediate the binding and 35 cell fusion of HIV and a cell. Exemplary cell surface receptors are the CD4 receptor, or the chemokine receptors, such as the CCR5 receptor, the CXCR4 receptor, or the WO 2008/019817 PCT/EP2007/007164 -18 CXCR5 receptor. Therefore in one embodiment is the antibody against an HIV gpl20 binding cell surface receptor an anti-CD4 antibody, or an anti-CCR5 antibody, or an anti-CXCR4 antibody, or an anti-CXCR5 antibody. The antibody used in the conjugate according to the invention is preferably 5 characterized in that the constant domains are of human origin. Such constant domains are well known in the state of the art and, e.g., described by Kabat (see e.g. Johnson, G., and Wu, T.T., Nucleic Acids Res. 28 (2000) 214-218). For example, a useful human IgG1 heavy chain constant region (CH1-Hinge-CH2-CH3) comprises an amino acid sequence independently selected from the group consisting of SEQ 10 ID NO: 26, 27. For example, a useful human kappa (K) light chain constant domain comprises an amino acid sequence of a kappa light chain constant domain (K light chain constant domain, CL) of SEQ ID NO: 28. It is further preferred that the antibody's variable domains are of mouse origin and comprises the antibody variable domain sequence frame of a mouse antibody according to Kabat (see e.g. 15 Johnson, G., and Wu, T.T., Nucleic Acids Res. 28 (2000) 214-218). A preferred anti-CCR5 antibody shows a binding to the same epitope(s) of CCR5 as does an antibody selected from the group consisting of the antibodies A to E or is inhibited in binding to CCR5 by antibodies A to E due to steric hindrance of binding or competitive binding. Epitope binding is investigated by using alanine 20 scanning according to the method described by Olson, W.C., et al. (J. Virol. 73 (1999) 4145-4155) for epitope mapping. A signal reduction of 75% or more shows that the mutated amino acid(s) contribute to the epitope recognized by said antibody. Binding of the antibody to the same epitope is found, if the amino acids contributing to the epitope are recognized by the investigated antibody and 25 antibody A, B, C, D, or E. Antibody C, which shows lower IC 5 0 values than antibody 2D7 in HIV assays, binds to an epitope including amino acids on the ECL2 domain of CCR5 (Lee, B., et al., J. Biol. Chem. 274 (1999) 9617-9626) which is different from the epitope recognized by antibody 2D7 (2D7 binds to amino acids K171 and E172 of ECL2A but not to ECL2B amino acids 184-189). Epitope binding for 30 antibody C is found to be 20% for CCR5 mutant K171A or E172A (glu 172 is mutated to ala). 100% epitope binding is defined for wild-type CCR5. A further preferred anti-CCR5 antibody binds to the same epitope as antibody C binds. The term "epitope" means a protein determinant capable of specific binding to an antibody. Epitopes usually consist of chemically active surface groupings of 35 molecules such as amino acids or sugar side chains and usually have specific three WO 2008/019817 PCT/EP2007/007164 - 19 dimensional structural characteristics, as well as specific charge characteristics. Conformational and non-conformational epitopes are distinguished in that the binding to the former but not the latter is lost in the presence of denaturing solvents. Preferably an antibody according to the invention binds specifically to 5 native but not to denatured CCR5. Such an antibody comprises preferably heavy chain CDR3 of SEQ ID NO: 17, and preferably in addition heavy chain CDRs selected from the group of CDRs of SEQ ID NO: 10, 11, 12, 14 and/or 15. Preferably such an antibody is antibody B, C, D, or E, or comprises the variable domains of antibody B, C, D, or E. Preferably an antibody binding to denatured 10 CCR5 is antibody A or comprises the variable domains of antibody A. The term "variable domain" (variable domain of a light chain (VL), variable domain of a heavy chain (VH)) as used herein denotes each domain of the pair of light and heavy chain domains which is involved directly in the binding of the antibody to the antigen. The variable domains of the light and heavy chain have the same 15 general structure, i.e. they possess an "immunoglobulin framework", and each domain comprises four "framework regions" (FR), whose sequences are widely conserved, connected by three "hypervariable regions" (or "complementarity determining regions", CDRs). The framework regions adopt a P-sheet conformation and the CDRs may form loops connecting the P-sheet structure. The 20 CDRs in each chain are held in their three-dimensional structure by the framework regions and form together with the CDRs from the other chain the antigen binding site. The antibody heavy and light chain CDR3 regions play a particularly important role in the binding specificity/affinity of the antibodies according to the invention and therefore provide a further object of the invention. 25 The terms "antigen-binding portion of an antibody" or "antigen-binding site of an antibody" when used herein refer to the amino acid residues of an antibody which are responsible for antigen-binding. The antigen-binding site of an antibody comprises amino acid residues from the "complementarity determining regions" or "CDRs". "Framework" or "FR" regions are those variable domain regions other 30 than the hypervariable region residues as herein defined. Therefore, the light and heavy chain variable domains of an antibody comprise from N- to C-terminus the regions FR1, CDR1, FR2, CDR2, FR3, CDR3, and FR4 (immunoglobulin framework). Especially, the CDR3 region of the heavy chain is the region which contributes most to antigen binding and defines the antibody. Preferably the anti 35 CCR5 antibody according to the invention is characterized by comprising in its heavy chain variable domain the CDR3 sequence of SEQ ID NO: 16 or SEQ ID NO: WO 2008/019817 PCT/EP2007/007164 - 20 17. Complementarity determining (CDR) and framework (FR) regions are determined according to the standard definition of Kabat, E.A., et al., Sequences of Proteins of Immunological Interest, 5th ed., Public Health Service, National Institutes of Health, Bethesda, MD (1991). 5 The "Fc part" of an anti-CCR5 antibody is not involved directly in binding to CCR5, but exhibit various effector functions. Depending on the amino acid sequence of the constant region of their heavy chains, antibodies or immunoglobulins are divided in the classes: IgA, IgD, IgE, IgG, and IgM, and several of these may be further divided into subclasses (isotypes), e.g. IgG1, IgG2, 10 IgG3, and IgG4, IgA1 and IgA2. According to the heavy chain constant regions the different classes of immunoglobulins are called oa, 5, s, y, and A respectively. The antibodies according to the invention are preferably of IgG type. An "Fc part of an antibody" is a term well known to the skilled artisan and defined on basis of papain cleavage of antibodies. The antibodies according to the invention contain as Fc part 15 a human Fc part or an Fc part derived from human origin. In a further embodiment of the invention the Fc part is either an Fc part of a human antibody of the subclass IgG4 or an Fc part of a human antibody of the subclass IgG1, IgG2, or IgG3, which is modified in such a way that no Fcy receptor (e.g. FcyRIIIa) binding and/or no Clq binding as defined below can be detected. Preferably the Fc 20 part is a human Fc part and especially preferred either from human IgG4 subclass or a mutated Fc part from human IgG1 subclass. Further preferred are Fc parts from human IgG1 subclass with mutations L234A and L235A. Further preferred are Fc parts shown in SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 26 with mutations L234A and L235A, SEQ ID NO: 27 with mutation S228P. While IgG4 shows 25 reduced Fcy receptor (FcyRIIIa) binding, antibodies of other IgG subclasses show strong binding. However Pro238, Asp265, Asp270, Asn297 (loss of Fc carbohydrate), Pro329, Leu234, Leu235, Gly236, Gly237, Ile253, Ser254, Lys288, Thr307, Gln311, Asn434, and His435 are residues which if altered provide also reduced Fcy receptor binding (Shields, R.L., et al., J. Biol. Chem. 276 (2001) 6591 30 6604; Lund, J., et al., FASEB J. 9 (1995) 115-119; Morgan, A., et al., Immunology 86 (1995) 319-324; EP 0 307434). Preferably an antibody according to the invention is in regard to Fcy receptor binding of IgG4 subclass or of IgG1 or IgG2 subclass, with a mutation in L234, L235, and/or D265, and/or contains the PVA236 mutation. Preferred are the mutations S228P, L234A, L235A, L235E, and/or 35 PVA236 (PVA236 means that the amino acid sequence ELLG (given in one letter amino acid code) from amino acid position 233 to 236 of IgG1 or EFLG of IgG4 is WO 2008/019817 PCT/EP2007/007164 -21 replaced by PVA). Especially preferred are the mutations S228P of IgG4, and L234A and L235A of IgG1. The Fc part of an antibody is directly involved in ADCC (antibody-dependent cell-mediated cytotoxicity) and CDC (complement dependent cytotoxicity). Complement activation (CDC) is initiated by binding of 5 complement factor Clq to the Fc part of most IgG antibody subclasses. Binding of Clq to an antibody is caused by defined protein-protein interactions at the so called binding site. Such Fc part binding sites are known in the state of the art and described e.g. by Lukas, T.J., et al., J. Immunol. 127 (1981) 2555-2560; Brunhouse, R., and Cebra, J.J., Mol. Immunol. 16 (1979) 907-917; Burton, D.R., et al., Nature 10 288 (1980) 338-344; Thommesen, J.E., et al., Mol. Immunol. 37 (2000) 995-1004; Idusogie, E.E., et al., J. Immunol. 164 (2000) 4178-4184; Hezareh, M., et al., J. Virol. 75 (2001) 12161-12168; Morgan, A., et al., Immunology 86 (1995) 319-324; and EP 0 307 434. Such Fc part binding sites are, e.g., characterized by the amino acids L234, L235, D270, N297, E318, K320, K322, P331, and P329 (numbering according 15 to EU index of Kabat). Antibodies of subclass IgGI, IgG2, and IgG3 usually show complement activation including Clq and C3 binding, whereas IgG4 does not activate the complement system and does not bind Clq and C3. An anti-CCR5 antibody which does not bind Fcy receptor and/or complement factor Clq does not elicit antibody-dependent cellular cytotoxicity (ADCC) and/or complement 20 dependent cytotoxicity (CDC). Preferably, this antibody is characterized in that it binds CCR5, contains an Fc part derived from human origin, and does not bind Fcy receptors and/or complement factor Clq. More preferably, this antibody is a human, or humanized, or a T-cell antigen depleted antibody. Clq binding can be measured according to Idusogie, E.E., et al., J. Immunol. 164 (2000) 4178-4184. No 25 "Clq binding" is found if in such an assay the optical density (OD) at 492-405 nm is for the test antibody lower than 15% of the value for human Clq binding of the unmodified wild-type antibody Fc part at an antibody concentration of 8 Pg/ml. ADCC can be measured as binding of the antibody to human FcyRIIIa on human NK cells. Binding is determined at an antibody concentration of 20 pg/ml. "No Fcy 30 receptor binding" or "no ADCC" means a binding of up to 30% to human FcyRIIIa on human NK cells at an antibody concentration of 20 ptg/ml compared to the binding of the same antibody as human IgG1 (SEQ ID NO:26). An antibody used in a conjugate according to the invention include, in addition, such antibodies having "conservative sequence modifications" (variant antibodies), 35 which are amino acid sequence modifications which do not affect or alter the above-mentioned characteristics of the antibody according to the invention.

WO 2008/019817 PCT/EP2007/007164 - 22 Modifications can be introduced by standard techniques known in the art, such as site-directed mutagenesis and PCR-mediated mutagenesis. Conservative amino acid substitutions include ones in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues 5 having similar side chains have been defined in the art. These families include amino acids with basic side chains (e.g. lysine, arginine, histidine), acidic side chains (e.g. aspartic acid, glutamic acid), uncharged polar side chains (e.g. glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine, tryptophan), non-polar side chains (e.g. alanine, valine, leucine, isoleucine, proline, phenylalanine, 10 methionine), beta-branched side chains (e.g. threonine, valine, isoleucine), and aromatic side chains (e.g. tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in a human anti-CCR5 antibody can be preferably replaced with another amino acid residue from the same side chain family. A "variant" anti-CCR5 antibody, refers therefore herein to a molecule which 15 differs in amino acid sequence from a "parent" anti-CCR5 antibody's amino acid sequence by up to ten, preferably from about two to about five, additions, deletions, and/or substitutions in one or more of the variable domain regions of the parent antibody outside the heavy chain CDR3 region. Each other heavy chain CDR region comprises at maximum one single amino acid addition, deletion, and/or 20 substitution. The invention comprises a method of modifying the CDR amino acid sequence of a parent antibody binding to CCR5, characterized in selecting a heavy chain variable domain from the group of heavy chain variable domains consisting of SEQ ID NO:1, 3, 5, 7, 82, 83 and/or a light chain variable domain from the group of light chain variable domains consisting of SEQ ID NO:2, 4, 6, 8, 76, 77 providing 25 a nucleic acid encoding said initial variable domain amino acid sequence, modifying said nucleic acid in that one amino acid is modified in heavy chain CDR1, one amino acid is modified in heavy chain CDR2, 1-3 amino acid are modified in light chain CDR1, 1-3 amino acids are modified in light chain CDR2, and/or 1-3 amino acids are modified in light chain CDR3, expressing and 30 incorporating said modified variable domain(s) amino acid sequence in an antibody structure, measuring whether said antibody binds to CCR5 and selecting said modified variable domain(s)/CDR(s) if the antibody binds to CCR5. Preferably such modifications are conservative sequence modifications. Amino acid sequence modifications can be performed by mutagenesis based on molecular modeling as 35 described by Riechmann, L., et al., Nature 332 (1988) 323-327, and Queen, C., et al., Proc. Natl. Acad. Sci. USA 86 (1989) 10029-10033.

WO 2008/019817 PCT/EP2007/007164 - 23 The term "linker" or "peptidic linker" as used within this application denotes peptide linkers of natural and/or synthetic origin. They are building up of a linear amino acid chain wherein the 20 naturally occurring amino acids are the monomeric building blocks. The chain has a length of from 1 to 50 amino acids, 5 preferred between 1 and 28 amino acids, especially preferred between 3 and 25 amino acids. The linker may contain repetitive amino acid sequences or sequences of naturally occurring polypeptides, such as polypeptides with a hinge-function. The linker has the function to ensure that a peptide conjugated to an anti-CCR5 antibody can perform its biological activity by allowing the peptide to fold correctly 10 and to be presented properly. Preferably the linker is a "synthetic peptidic linker" that is designated to be rich in glycine, glutamine, and/or serine residues. These residues are arranged e.g. in small repetitive units of up to five amino acids, such as GGGGS, QQQQG, or SSSSG. This small repetitive unit may be repeated for two to five times to form a multimeric unit. At the amino- and/or carboxy-terminal ends 15 of the multimeric unit up to six additional arbitrary, naturally occurring amino acids may be added. Other synthetic peptidic linkers are composed of a single amino acid, that is repeated between 10 to 20 times, such as e.g. serine in the linker SSSSSSSSSSSSSSS. At each of the amino- and/or carboxy-terminal end up to six additional arbitrary, naturally occurring amino acids may be present. Preferred 20 linkers are shown in Table 2. Especially preferred are linkers [GQ 4

]

3 GNN (SEQ ID NO:40), LSLSPGK (SEQ ID NO:36), LSPNRGEC (SEQ ID NO:37), LSLSGG (SEQ ID NO:61), LSLSPGG (SEQ ID NO:62), G 3

[SG

4

]

2 SG (SEQ ID NO:69). All peptidic linkers can be encoded by a nucleic acid molecule and therefore can be recombinantly expressed. As the linkers are themselves peptides, the antifusogenic 25 peptide is connected to the linker via a peptide bond that is formed between two amino acids. The peptidic linker is introduced between the antifusogenic peptide and the anti-CCR5 antibody chain to which the antifusogenic peptide is to be conjugated. Therefore two or three, respectively, possible sequences (in amino- to carboxy-terminal direction) exist: a) antifusogenic peptide - peptidic linker - anti 30 CCR5 antibody polypeptide chain, or b) anti-CCR5 antibody polypeptide chain peptidic linker - antifusogenic peptide, or c) antifusogenic peptide - peptidic linker - anti-CCR5 antibody polypeptide chain - peptidic linker - antifusogenic peptide. In one embodiment of the invention the mAb CCR5-conjugate is characterized in 35 comprising i) the heavy chain (VH) variable domain defined by amino acid sequence SEQ ID NO: 1 and the light chain (VL) variable domain defined by SEQ ID WO 2008/019817 PCT/EP2007/007164 - 24 NO:2; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:3 and the light chain variable domain defined by SEQ ID NO:4; or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:5 and the light chain variable domain defined by SEQ ID NO:6; or the heavy chain variable 5 domain defined by amino acid sequence SEQ ID NO:7 and the light chain variable domain defined by SEQ ID NO:8; ii) a linker selected from the group consisting of the amino acids glycine (G) and asparagine (N), the tripeptide GST, and SEQ ID NO:36-62, and SEQ ID NO:67-70; and iii) an antifusogenic peptide selected from the group of peptides defined by SEQ ID NO:29 to 35, or 73. 10 A preferred conjugate of a heavy and/or light chain of mAb CCR5 and an antifusogenic peptide(s) ("chain conjugate") is selected from the group consisting of the conjugates (1) [antifusogenic peptide] - [linkerim - [heavy chain], (2) [heavy chain] - [linker]m - [antifusogenic peptide], (3) [antifusogenic peptide] - [linker]m - [heavy chain] - [antifusogenic peptide], (4) [antifusogenic peptide] - [linker]m 15 [light chain], (5) [light chain] - [linker]m - [antifusogenic peptide], (6) [antifusogenic peptide] - [linker]m- [light chain] - [antifusogenic peptide], (7) [antifusogenic peptide] - [linker]m- [heavy chain] - [linker]m - [antifusogenic peptide], (8) [antifusogenic peptide] - [linker]m - [light chain] - [linker]m [antifusogenic peptide], wherein the linker can be the same or different both within 20 and between said chain conjugates, wherein m is an integer of 1 or 0, and m can be independently the same or different both within and between said conjugates. For example in a conjugate comprising a chain conjugate (7) and a mAb CCR5 light chain the two linkers in chain conjugate (7) can be the same, i.e. have the same amino acid sequence and length, or can be different, i.e. have different amino acid 25 sequences and/or lengths, or one or both can be absent. For example in a conjugate comprising chain conjugates (2) and (4) the linker contained in chain conjugate (2) and the linker contained in chain conjugate (4) can be the same, i.e. have the same amino acid sequence and length, or can be different, i.e. have different amino acid sequences and/or lengths, or one or both can be absent. In the chain conjugates the 30 linker(s) can be present (m=1) or absent (m=O). Preferred chain conjugates are the chain conjugates (2), (3), (4), and (7). One embodiment of the current invention is a conjugate comprising 2 x [mAb CCR5 light chain] and 2 x chain conjugate (2). This conjugate comprises two not conjugate anti-CCR5 antibody light chains and two anti-CCR5 antibody heavy chains conjugated via the C-terminus to the N 35 terminus of an antifusogenic peptide, optionally with an intermediate linker. Another embodiment of the current invention is a conjugate comprising two mAb WO 2008/019817 PCT/EP2007/007164 - 25 CCR5 light chains and two chain conjugates (3). Still another embodiment is a conjugate comprising two mAb CCR5 heavy chains and two chain conjugates (4). A further embodiment of the current invention is a conjugate comprising two mAb CCR5 light chains and two chain conjugates (7). The heavy and/or light chain 5 comprises preferably a constant region (Fc). The invention further provides a method for the manufacture of a pharmaceutical composition comprising an effective amount of a conjugate according to the invention together with a pharmaceutically acceptable carrier and the use of the conjugate according to the invention for such a method. 10 The invention further provides the use of a conjugate according to the invention in an effective amount for the manufacture of a pharmaceutical agent, preferably together with a pharmaceutically acceptable carrier, for the treatment of a patient suffering from AIDS. The term "amino acid" as used within this application denotes the group of 15 naturally occurring carboxy oa-amino acids comprising alanine (three letter code: ala, one letter code: A), arginine (arg, R), asparagine (asn, N), aspartic acid (asp, D), cysteine (cys, C), glutamine (gln, Q), glutamic acid (glu, E), glycine (gly, G), histidine (his, H), isoleucine (ile, I), leucine (leu, L), lysine (lys, K), methionine (met, M), phenylalanine (phe, F), proline (pro, P), serine (ser, S), threonine (thr, 20 T), tryptophan (trp, W), tyrosine (tyr, Y), and valine (val, V). Methods and techniques known to a person skilled in the art, which are useful for carrying out the current invention, are described e.g. in Ausubel, F.M., ed., Current Protocols in Molecular Biology, Volumes I to III (1997), Wiley and Sons; Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring 25 Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989). In the conjugates according to the invention the carboxy-terminal amino acid of an antibody chain is conjugated via a peptide bond to the amino-terminal amino acid of the antifusogenic peptide or the carboxy-terminal amino acid of the antifusogenic peptide is conjugated via a peptide bond to the amino-terminal 30 amino acid of an antibody chain. In one embodiment an intermediate linker is present between the antifusogenic peptide and the antibody chain. Thus, the conjugate according to the invention is characterized by the general formula antibody - [linker]m - [antifusogenic peptide]n WO 2008/019817 PCT/EP2007/007164 - 26 wherein m is independently for each antifusogenic peptide either 0 (i.e. a direct peptide bond between antibody and the antifusogenic peptide) or 1 (i.e. a linker is present between antibody and antifusogenic peptide) and n is an integer of from 1 to 8. In one embodiment n is an integer of from 2 to 8. In another embodiment n is 5 an integer of from 2 to 4. In another embodiment n is an integer of 2 or 4. One embodiment of the invention comprises a conjugate characterized in comprising an antifusogenic peptide at each C-terminus of the heavy chains or at each N-terminus of the light chains of the antibody. In this embodiment two antifusogenic peptides are conjugated to one antibody. In an other embodiment is the conjugate 10 characterized by comprising an antifusogenic peptide at each C-terminus of the heavy chains and at each N-terminus of the light chains. In this embodiment four antifusogenic peptides are conjugated to one antibody. In one embodiment is said antibody mAb CCR5 or mAb CD4. The antifusogenic peptide which is introduced at a terminus of a mAb CCR5 heavy 15 and/or light chain(s) is small of size compared to the mAb CCR5. For example, the smallest immunoglobulins, immunoglobulins of class G, have a molecular weight of approximately 150 kDa; an antifusogenic peptide has preferably a size (molecular weight) of less than 12.5 kDa, which is equivalent to about 100 amino acids, in general less than 7.5 kDa, which is equivalent to about 60 amino acids. The 20 antifusogenic peptide has an amino acid sequence of from 5 to 100 amino acid residues, preferably of from 10 to 75 amino acid residues, more preferably of from 15 to 50 amino acid residues. The conjugates of the current invention are useful for pharmaceutical, therapeutical, or diagnostical applications. The number of antifusogenic peptides, which can be conjugated to mAb CCR5 heavy and/or light 25 chain(s), is from one to the combined number of amino- and carboxy-termini of the anti-CCR5 antibody polypeptide chains. As the current invention encompasses different anti-CCR5 antibodies the number of antifusogenic peptides can vary. In case of an anti-CCR5 antibody comprising two heavy and two light chains the combined number of amino-termini (N-termini) and carboxy-termini (C-termini) 30 is eight, which is at the same time the totaling maximum number of conjugated antifusogenic peptides; in case e.g. of an anti-CCR5 antibody fragment such as a single chain antibody (scFv) the combined number of termini and therefore the maximum number of conjugatable antifusogenic peptides is two. If a single antifusogenic peptide is conjugated to mAb CCR5, the peptide can occupy any one 35 of the termini of the anti-CCR5 antibody chains. Likewise, if the maximum possible number of peptides is conjugated to mAb CCR5, all termini are occupied by a WO 2008/019817 PCT/EP2007/007164 - 27 single peptide. If the number of peptides, which are conjugated to mAb CCR5, is smaller than the maximum possible number, different distributions of the peptides at the termini of the anti-CCR5 antibody chains are possible. For example, if four peptides are conjugated to an immunoglobulin of the G or E class, five different 5 combinations are possible (see Table 1). In two combinations all termini of one kind, i.e. all four amino-termini or all four carboxy-termini of the anti-CCR5 antibody chains, are each conjugated to one single antifusogenic peptide. The other termini are not conjugated. This results in one embodiment in an allocation of the modifications/conjugations in one area of the anti-CCR5 antibody. In the other 10 cases the polypeptides are conjugated to a number of both termini. Within these combinations the conjugated peptides are allocated to different areas of the anti CCR5 antibody. In either case the sum of conjugated termini is four. Table 1: Possible combination for the conjugation of four peptides to the termini of an anti-CCR5 antibody composed of four polypeptide chains. number of occupied number of occupied total number of amino-termini carboxy-termini occupied termini 4 0 4 3 1 4 2 2 4 1 3 4 0 4 4 15 The current invention preferably comprises conjugates in which at least two of the termini are conjugated to an antifusogenic peptide. The amino acid sequences of the antifusogenic peptides can be different, similar or identical. In one embodiment the amino acid sequence identity is in the range of from 90% to less than 100%; 20 these amino acid sequences and the corresponding peptides are defined as similar. In a preferred embodiment the antifusogenic peptides are identical, i.e. have an amino acid identity of 100%. The present invention comprises a conjugate comprising one or more antifusogenic peptides and an anti-CCR5 antibody (mAb CCR5) wherein one to eight 25 antifusogenic peptides are each conjugated to one terminus of the heavy and/or light chains of said anti-CCR5 antibody via a peptide bond. In one embodiment the conjugate according to the invention comprises at least two antifusogenic peptides and an anti-CCR5 antibody wherein two to eight antifusogenic peptides are each WO 2008/019817 PCT/EP2007/007164 - 28 conjugated to one terminus of the heavy and/or light chains of said anti-CCR5 antibody. In one embodiment the conjugate according to the invention is characterized i) in comprising two light chain variable domains of SEQ ID NO:2, two chain conjugates 5 of type (2) each comprising a heavy chain variable domain of SEQ ID NO:1, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, ii) in comprising two light chain variable domains of SEQ ID NO:4, two chain conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:3, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, iii) in 10 comprising two light chain variable domains of SEQ ID NO:6, two chain conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:5, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, or iv) in comprising two light chain variable domains of SEQ ID NO:8, two chain conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:7, a 15 linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33. The conjugation between the antifusogenic peptide and the anti-CCR5 antibody is performed on the nucleic acid level. Therefore a peptide bond is formed between the antifusogenic peptide and the anti-CCR5 antibody chain with or without an intermediate linker. Thus either the carboxy-terminal amino acid of the 20 antifusogenic peptide is conjugated to the amino-terminal amino acid of an anti CCR5 antibody chain with or without an intermediate linker, or a carboxy-terminal amino acid of the anti-CCR5 antibody chain is conjugated to the amino-terminal amino acid of the antifusogenic peptide with or without an intermediate linker or both termini of the anti-CCR5 antibody chain are conjugated to an antifusogenic 25 peptide each with or without an intermediate linker. For the recombinant production of the antifusogenic peptide-anti-CCR5 antibody-conjugate according to the invention one or more nucleic acid molecules encoding different polypeptides are required, preferably two to eight nucleic acid molecules are employed. These nucleic acid molecules encode the different anti-CCR5 antibody 30 polypeptide chains of the conjugate and are in the following referred to as structural genes. They can be located on the same expression plasmid (vector) or can alternatively be located on different expression plasmids (vectors). The assembly of the conjugate takes preferably place before secretion of the conjugate and thus within the expressing cells. Therefore the nucleic acid molecules encoding the 35 polypeptide chains of the conjugate are preferably expressed in the same host cell. If after recombinant expression a mixture of conjugates is obtained, the conjugates WO 2008/019817 PCT/EP2007/007164 - 29 can be separated and purified by methods known to a person skilled in the art. These methods are well established and widespread used for immunoglobulin purification and are employed either alone or in combination. Such methods are, for example, affinity chromatography using microbial-derived proteins (e.g. protein 5 A or protein G affinity chromatography), ion exchange chromatography (e.g. cation exchange (carboxymethyl resins), anion exchange (amino ethyl resins) and mixed-mode exchange chromatography), thiophilic adsorption (e.g. with beta mercaptoethanol and other SH ligands), hydrophobic interaction or aromatic adsorption chromatography (e.g. with phenyl-sepharose, aza-arenophilic resins, or 10 m-aminophenylboronic acid), metal chelate affinity chromatography (e.g. with Ni(II)- and Cu(II)-affinity material), size exclusion chromatography, and preparative electrophoretic methods (such as gel electrophoresis, capillary electrophoresis) (Vijayalakshmi, M.A., Appl. Biochem. Biotech. 75 (1998) 93-102). With recombinant engineering methods known to a person skilled in the art the 15 conjugates can be tailor-made on the nucleic acid/gene level. The nucleic acid sequences encoding immunoglobulins are known and can be obtained for example from genomic databases. Likewise the nucleic acid sequences encoding antifusogenic peptides are known or can easily be deduced from their amino acid sequence. The elements required for the construction of an expression plasmid for 20 the expression of the conjugate of the current invention are, for example, an expression cassette for the anti-CCR5 antibody light chain in its natural and/or modified and/or conjugated version, an expression cassette for the anti-CCR5 antibody heavy chain in its natural and/or modified and/or conjugated version (alternatively the anti-CCR5 antibody light chain and the anti-CCR5 antibody 25 heavy chain can be contained in the same expression cassette, e.g. as bicistronic expression element), a selection marker, and an E.coli replication as well as selection unit. These expression cassettes comprise a promoter, a DNA segment encoding a secretion signal sequence, the structural gene, and a terminator/polyadenylation signal. The elements are assembled in an operatively 30 linked form either on one plasmid encoding all chains of the conjugate, or on two or more plasmids each encoding one or more chains of the conjugate. For the expression of the encoded polypeptides the plasmid(s) is (are) introduced into a suitable host cell. Proteins are preferably produced in mammalian cells such as CHO cells, NSO cells, Sp2/0 cells, COS cells, HEK cells, K562 cells, BHK cells, 35 PER.C6® cells, and the like. The regulatory elements of the plasmid have to be selected in a way that they are functional in the selected host cell. For the expression the host cell containing the plasmid encoding one or more chains of the conjugate WO 2008/019817 PCT/EP2007/007164 - 30 is cultivated under conditions suitable for the expression of the chains. The expressed conjugate chains are functionally assembled. The fully processed antifusogenic peptide-anti-CCR5 antibody-conjugate is secreted into the medium. An "expression plasmid" is a nucleic acid encoding a polypeptide to be expressed in 5 a host cell. Typically, an expression plasmid comprises a prokaryotic plasmid propagation unit, e.g. for E.coli, comprising an origin of replication, and a resistance gene, an eukaryotic selection marker, and one or more expression cassettes for the expression of the structural gene(s) of interest comprising a promoter, a structural gene, and a transcription terminator including a 10 polyadenylation signal. Gene expression is usually placed under the control of a promoter, and such a structural gene is said to be "operably linked to" the promoter. Similarly, a regulatory element and a core promoter are operably linked if the regulatory element modulates the activity of the core promoter. One aspect of the current invention is thus a method for the production of a 15 conjugate according to the invention, comprising the following steps a) cultivating a cell containing one or more expression plasmids each comprising one or more nucleic acid molecules encoding a conjugate according to the invention under conditions suitable for the expression of the conjugate, 20 b) recovering the conjugate from the cell or the supernatant. The term "under conditions suitable for the expression of the conjugate" denotes conditions which are used for the cultivation of a cell expressing a polypeptide and which are known to or can easily be determined by a person skilled in the art. It is 25 known to a person skilled in the art that these conditions may vary depending on the type of cell cultivated and type of polypeptide expressed. In general the cell is cultivated at a temperature, e.g. between 20 0 C and 40'C, and for a period of time sufficient to allow effective production of the polypeptide conjugate, e.g. for 4 to 28 days. 30 As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption/resorption delaying agents, and the like that are physiologically compatible. Preferably, the carrier is suitable for injection or infusion. Pharmaceutically acceptable carriers include sterile aqueous solutions or 35 dispersions and sterile powders for the preparation of sterile injectable solutions or WO 2008/019817 PCT/EP2007/007164 -31 dispersion. The use of such media and agents for pharmaceutically active substances is known in the art. In addition to water, the carrier can be, for example, an isotonic buffered saline solution. Regardless of the route of administration selected, the compounds of the present 5 invention, which may be used in a suitable hydrated form, and/or the pharmaceutical compositions of the present invention, are formulated into pharmaceutically acceptable dosage forms by conventional methods known to those of skilled in the art. Actual dosage levels of the active ingredients in the pharmaceutical compositions of 10 the present invention may be varied so as to obtain an amount of the active ingredient, which is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration, without being toxic to the patient. The selected dosage level will depend upon a variety of pharmacokinetic factors including the activity of the particular compositions of the 15 present invention employed, the route of administration, the time of administration, the rate of excretion of the particular compound being employed, other drugs, compounds and/or materials used in combination with the particular compositions employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and like factors well known in the 20 medical arts. The invention preferably comprises the use of a conjugate according to the invention for the treatment of a patient suffering from immunodeficiency syndromes such as AIDS. The following examples, sequence listing, figures and deposits are provided to aid 25 the understanding of the present invention, the true scope of which is set forth in the appended claims. It is understood that modifications can be made in the procedures set forth without departing from the spirit of the invention. Description of the Figures Figure 1: Plasmid map of mAb CCR5 K-light chain expression vector 4900. 30 Figure 2: Plasmid map of mAb CCR5 yl-heavy chain expression vector 4901. Figure 3: Plasmid map of mAb CCR5 yl-heavy chain conjugate expression vector 4995. Figure 4: Plasmid map of mAb CD4 K-light chain expression vector 6310.

WO 2008/019817 PCT/EP2007/007164 - 32 Figure 5: Plasmid map of mAb CD4 yl-heavy chain expression vector 6309. Figure 6: Plasmid map of mAb CD4 yl-heavy chain conjugate expression vector 6303. Anti-CCR5 antibody deposition 5 Preferred hybridoma cell lines expressing mAb CCR5 useful in the conjugates according to the invention were deposited with Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Germany. Cell line Deposition No. Date of Deposit m<CCR5>Pz01.F3 DSM ACC 2681 18.08.2004 m<CCR5>Pz02.1C11 DSM ACC 2682 18.08.2004 m<CCR5>Pz03.1C5 DSM ACC 2683 18.08.2004 m<CCR5>Pz04.1F6 DSM ACC 2684 18.08.2004 Antibody nomenclature 10 <CCR5>Pz01.F3: Antibody A SEQ ID NO: 1, 2 <CCR5>Pz02.1C11: Antibody B SEQ ID NO: 3, 4 <CCR5>Pz03.1C5: Antibody C SEQ ID NO: 5, 6 <CCR5>F3.1H12.2E5: Antibody D SEQ ID NO: 7, 8 <CCR5>Pz04.1F6: Antibody E 15 Variable domains 1 of mAb CD4 are reported in SEQ ID NO: 10, 15, 45, and 56 of US 5,871,732. Variable domains 2 and 4 of mAb CD4 are reported in Reimann, K.A., et al., Aids Res. Human Retrovir. 13 (1997) 933-943. Variable domain 3 of mAb CD4 are reported in Figures 3, 4, 12, and 13 of 20 WO 91/009966. Antibody sequences, sequences of antifusogenic peptides and sequences of peptidic linkers SEQ ID NO: 1 <CCR5>PzOl.F3 heavy chain, variable domain 25 SEQ ID NO: 2 <CCR5>PzOl.F3 light chain, variable domain SEQ ID NO: 3 <CCR5>Pz02.1C11 heavy chain, variable domain SEQ ID NO: 4 <CCR5>Pz02.1C11 light chain, variable domain SEQ ID NO: 5 <CCR5>Pz03.1C5 heavy chain, variable domain SEQ ID NO: 6 <CCR5>Pz03.1C5 light chain, variable domain 30 SEQ ID NO: 7 <CCR5>F3.1H12.2E5 heavy chain, variable domain SEQ ID NO: 8 <CCR5>F3.1H12.2E5 light chain, variable domain WO 2008/019817 PCT/EP2007/007164 - 33 SEQ ID NO: 9 Heavy chain CDR1 mAb CCR5 SEQ ID NO:10 Heavy chain CDR1 mAb CCR5 SEQ ID NO: 11 Heavy chain CDR1 mAb CCR5 SEQ ID NO:12 Heavy chain CDR1 mAb CCR5 5 SEQ ID NO:13 Heavy chain CDR2 mAb CCR5 SEQ ID NO:14 Heavy chain CDR2 mAb CCR5 SEQ ID NO:15 Heavy chain CDR2 mAb CCR5 SEQ ID NO:16 Heavy chain CDR3 mAb CCR5 SEQ ID NO:17 Heavy chain CDR3 mAb CCR5 10 SEQ ID NO:18 Light chain CDR1 mAb CCR5 SEQ ID NO:19 Light chain CDR1 mAb CCR5 SEQ ID NO:20 Light chain CDR1 mAb CCR5 SEQ ID NO:21 Light chain CDR2 mAb CCR5 SEQ ID NO:22 Light chain CDR2 mAb CCR5 15 SEQ ID NO:23 Light chain CDR2 mAb CCR5 SEQ ID NO:24 Light chain CDR3 mAb CCR5 SEQ ID NO:25 Light chain CDR3 mAb CCR5 SEQ ID NO:26 y71 heavy chain constant region SEQ ID NO:27 74 heavy chain constant region 20 SEQ ID NO:28 K light chain constant domain SEQ ID NO:29 C34 SEQ ID NO:30 T20 SEQ ID NO:31 T1249 SEQ ID NO:32 T651 25 SEQ ID NO:33 T2635 SEQ ID NO:34 N36 SEQ ID NO:35 DP107 SEQ ID NO:36-62, 67-70 linker peptides SEQ ID NO:63 Amino acid sequence of mature mAb CCR5 K-light chain 30 SEQ ID NO:64 Amino acid sequence of mature mAb CCR5 yl-heavy chain SEQ ID NO:65 Amino acid sequence of mature mAb CCR5 conjugate heavy chain SEQ ID NO:66 HIV-1 gp41 SEQ ID NO:71 Amino acid sequence of mature mAb CD4 i-light chain 35 SEQ ID NO:72 Amino acid sequence of mature mAb CD4 yl-heavy chain SEQ ID NO:73 afp-1 WO 2008/019817 PCT/EP2007/007164 - 34 SEQ ID NO:74 Amino acid sequence of mature mAb CD4 conjugate heavy chain SEQ ID NO:75 murine light chain variable domain 1 mAb CD4 SEQ ID NO:76 chimeric light chain variable domain 1 mAb CD4 5 SEQ ID NO:77 chimeric light chain 1 mAb CD4 SEQ ID NO:78 chimeric light chain variable domain 2 mAb CD4 SEQ ID NO:79 chimeric light chain variable domain 3 mAb CD4 SEQ ID NO:80 chimeric light chain variable domain 4 mAb CD4 SEQ ID NO:81 murine heavy chain variable domain 1 mAb CD4 10 SEQ ID NO:82 chimeric heavy chain variable domain 1 mAb CD4 SEQ ID NO:83 chimeric heavy chain 1 mAb CD4 SEQ ID NO:84 chimeric heavy chain variable domain 2 mAb CD4 SEQ ID NO:85 chimeric heavy chain variable domain 3 mAb CD4 SEQ ID NO:86 chimeric heavy chain variable domain 4 mAb CD4 15 SEQ ID NO:87 light chain CDR1 mAb CD4 SEQ ID NO:88 light chain CDR1 mAb CD4 SEQ ID NO:89 light chain CDR1 mAb CD4 SEQ ID NO:90 light chain CDR1 mAb CD4 SEQ ID NO:91 light chain CDR2 mAb CD4 20 SEQ ID NO:92 light chain CDR2 mAb CD4 SEQ ID NO:93 light chain CDR2 mAb CD4 SEQ ID NO:94 light chain CDR3 mAb CD4 SEQ ID NO:95 light chain CDR3 mAb CD4 SEQ ID NO:96 light chain CDR3 mAb CD4 25 SEQ ID NO:97 heavy chain CDR1 mAb CD4 SEQ ID NO:98 heavy chain CDR1 mAb CD4 SEQ ID NO:99 heavy chain CDR1 mAb CD4 SEQ ID NO:100 heavy chain CDR2 mAb CD4 SEQ ID NO:101 heavy chain CDR2 mAb CD4 30 SEQ ID NO:102 heavy chain CDR2 mAb CD4 SEQ ID NO:103 heavy chain CDR3 mAb CD4 SEQ ID NO:104 heavy chain CDR3 mAb CD4 SEQ ID NO:105 heavy chain CDR3 mAb CD4 WO 2008/019817 PCT/EP2007/007164 -35 Table 2: Linker No. Linker peptides SEQ ID NO: 1 LSLSPGK 36 2 LSPNRGEC 37 3 [GQ 4 1 3 38 4 [GQ 4

]

3 G 39 5 [GQ 4

]

3 GNN 40 6 GGG[SG4] 2 SGG 41 7 GGG[SG 4

]

2 SGN 42 8 [SG 4 13 43 9 [SG 4

]

3 G 44 10 G[SG 4

]

3 T 45 11 [SG 4 ]3GG 46 12 [SG 4

]

3 GGT 47 13 [SG 4

]

3 GGN 48 14 [SG 4

]

3 GAS 49 15 [SG 4 1 5 50 16 [SG 4

]

5 G 51 17 [SG 4

]

5 GG 52 18 [SG 4

]

5 GAS 53 19 G(S)s 5 G 54 20 G(S)s 5 GAS 55 21 G 22 N 23 GST 24 [(G) 4

S]

3 GAS 56 25 [(G) 4

S]

3 G 57 26 [(G) 4 S]sG 58 27 [(G) 4

S]

3 GG 59 28 [(G) 4 S]sGG 60 29 LSLSGG 61 30 LSLSPGG 62 31 [G 3 S]s 67 32 [G 3 S]sGGG 68 33 G 3

[SG

4

]

2 SG 69 34 G 3

[SG

4 ] 2

SG

2 70 WO 2008/019817 PCT/EP2007/007164 - 36 Examples Materials & Methods General information regarding the nucleotide sequences of human immunoglobulins light and heavy chains is given in: Kabat, E.A., et al., Sequences of 5 Proteins of Immunological Interest, 5th ed., Public Health Service, National Institutes of Health, Bethesda, MD (1991). Amino acids of antibody chains are numbered according to EU numbering (Edelman, G.M., et al., Proc. Natl. Acad. Sci. USA 63 (1969) 78-85; Kabat, E.A., et al., Sequences of Proteins of Immunological Interest,5th ed., Public Health Service, National Institutes of 10 Health, Bethesda, MD, (1991)). Recombinant DNA techniques: Standard methods were used to manipulate DNA as described in Sambrook, J. et al., Molecular cloning: A laboratory manual; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989. The molecular biological reagents were used 15 according to the manufacturer's instructions. Gene synthesis: Desired gene segments were prepared from oligonucleotides made by chemical synthesis. The 100 - 600 bp long gene segments, which are flanked by singular restriction endonuclease cleavage sites, were assembled by annealing and ligation of 20 oligonucleotides including PCR amplification and subsequently cloned into the pCR2.1-TOPO-TA cloning vector (Invitrogen Corp., USA) via A-overhangs. The DNA sequence of the subcloned gene fragments were confirmed by DNA sequencing. Protein determination: 25 The protein concentration of the conjugate was determined by determining the optical density (OD) at 280 nm, using the molar extinction coefficient calculated on the basis of the amino acid sequence. Example 1 Making of the anti-CCR5 antibody expression plasmids 30 The gene segments encoding an anti-CCR5 antibody light chain variable domain (VL) and the human kappa-light chain constant domain (CL) were joined as were gene segments for the anti-CCR5 antibody heavy chain variable domain (VH) and the human yl-heavy chain constant domains (CH1-Hinge-CH2-CH3).

WO 2008/019817 PCT/EP2007/007164 - 37 In the case of mAb CCR5 of SEQ ID NO:63/64 the heavy and light chain variable domains are derived from a mouse antibody and the heavy and light chain constant domains are derived from a human antibody (C-kappa and IgG1). Subsequently, the gene segment encoding a complete anti-CCR5 antibody light 5 chain was joined at the N- and/or C-terminus with a nucleic acid encoding an antifusogenic peptide including a connecting linker sequence and/or the gene segment encoding a complete anti-CCR5 antibody heavy chain was joined at the N and/or C-terminus with a nucleic acid encoding an antifusogenic peptide including a connecting linker sequence. 10 a) Vector 4900 Vector 4900 is an expression plasmid for transient expression of a mAb CCR5 light chain (genomically organized expression cassette; exon-intron organization) in HEK293 cells. Beside the mAb CCR5 K-light chain expression cassette this vector contains: 15 - a hygromycine resistance gene as a selectable marker, - an origin of replication, oriP, of Epstein-Barr virus (EBV), - an origin of replication from the vector pUC18 which allows replication of this plasmid in E. coli, and - a 8-lactamase gene which confers ampicillin resistance in E. coli. 20 The transcription unit of the mAb CCR5 K-light chain gene is composed of the following elements: - the immediate early enhancer and promoter from the human cytomegalovirus, - a synthetic 5'-untranslated region, 25 - a murine immunoglobulin heavy chain signal sequence including a signal sequence intron (signal sequence 1, intron, signal sequence 2 [L1-intron-L2]), - the murine anti-CCR5 antibody mature variable K-light chain encoding segment arranged with a unique BsmI restriction site at the 5'-end (L2 signal sequence) and a splice donor site and a unique NotI restriction site at the 3' 30 end, - a human/mouse K-light chain hybrid intron 2, - the human K-light gene constant domain, - the human immunoglobulin K-polyadenylation ("poly A") signal sequence, and 35 - the unique restriction sites AscI and FseI at the 5'- and 3'-end, respectively.

WO 2008/019817 PCT/EP2007/007164 - 38 The plasmid map of the mAb CCR5 K-light chain expression vector 4900 is shown in Figure 1. The amino acid sequence of the mature (without signal sequence) mAb CCR5 K-light chain is shown in SEQ ID NO:63. 5 b) Vector 4991 Vector 4991 is an expression plasmid for transient expression of a mAb CCR5 y71 heavy chain (genomically organized expression cassette; exon-intron organization) in HEK293 cells. Beside the mAb CCR5 yl-heavy chain expression cassette this vector contains: 10 - a hygromycin resistance gene as a selectable marker, - an origin of replication, oriP, of Epstein-Barr virus (EBV), - an origin of replication from the vector pUC18 which allows replication of this plasmid in E. coli, and - a beta-lactamase gene which confers ampicillin resistance in E. coli. 15 The transcription unit of the mAb CCR5 yl-heavy chain is composed of the following elements: - the immediate early enhancer and promoter from the human cytomegalovirus, - a synthetic 5'-untranslated region, 20 - a murine immunoglobulin heavy chain signal sequence including a signal sequence intron (signal sequence 1, intron, signal sequence 2 [L1-intron-L2]), - the murine anti-CCR5 antibody mature variable heavy chain encoding segment arranged with a unique BsmI restriction site at the 5'-end (L2 signal sequence) and a splice donor site and a unique NotI restriction site at the 3'-end, 25 - a human/mouse heavy chain hybrid intron 2 including the mouse heavy chain enhancer element (part JH 3 , JH 4 ) (Neuberger, M.S., EMBO J. 2 (1983) 1373 1378), - the genomic human yl-heavy gene constant domains, - the human yl-immunoglobulin polyadenylation ("poly A") signal sequence, 30 and - the unique restriction sites AscI and SgrAI at the 5'- and 3'-end, respectively. The plasmid map of the mAb CCR5 yl-heavy chain expression vector 4901 is shown in Figure 2. The amino acid sequence of the mature (without signal 35 sequence) mAb CCR5 yl-heavy chain is shown in SEQ ID NO:64.

WO 2008/019817 PCT/EP2007/007164 - 39 c) Vector 4995 Vector 4995 is an expression plasmid for transient expression of a chimeric peptide anti-CCR5 antibody yl-heavy chain conjugate (genomically organized expression cassette; exon-intron organization) in HEK293 cells. 5 The vector 4995 is derived from plasmid 4991 in that way that the mAb CCR5 y1 heavy chain is joint at the C-terminus with a nucleic acid encoding the antifusogenic peptide T-2635 (SEQ ID NO:33) and the peptidic linker sequence

[GQ

4

]

3 GNN (SEQ ID NO:40). Beside the chimeric peptide anti-CCR5 antibody yl-heavy chain conjugate 10 expression cassette this vector contains: - a hygromycin resistance gene as a selectable marker, - an origin of replication, oriP, of Epstein-Barr virus (EBV), - an origin of replication from the vector pUC18 which allows replication of this plasmid in E. coli, and 15 - a beta(3)-lactamase gene which confers ampicillin resistance in E. coli. The transcription unit of the chimeric peptide-anti-CCR5 antibody yl-heavy chain conjugate is composed of the following elements: - the immediate early enhancer and promoter from the human cytomegalovirus, 20 - a synthetic 5'-untranslated region, - a murine immunoglobulin heavy chain signal sequence including a signal sequence intron (signal sequence 1, intron, signal sequence 2 [L1-intron-L2]), - the murine anti-CCR5 antibody mature variable heavy chain encoding segment arranged with a unique BsmI restriction site at the 5'-end (L2 signal sequence) 25 and a splice donor site and a unique NotI restriction site at the 3'-end, - a human/mouse heavy chain hybrid intron 2 including the mouse heavy chain enhancer element (part JH 3 , JH 4 ) (Neuberger, M.S., EMBO J. 2 (1983) 1373 1378), - the genomic human yl-heavy gene constant domains, 30 - the antifusogenic peptide T-2635, - the peptidic linker sequence [GQ 4

]

3 GNN, - the human yl-immunoglobulin polyadenylation ("poly A") signal sequence, and - the unique restriction sites AscI and SgrAI at the 5'- and 3'-end, respectively. 35 WO 2008/019817 PCT/EP2007/007164 - 40 The plasmid map of the mAb CCR5 yl-heavy chain conjugate expression vector 4995 is shown in Figure 3. The amino acid sequence of the mature (without signal sequence) conjugate heavy chain is shown in SEQ ID NO:65. Example 2 5 Making of the final anti-CCR5 antibody expression plasmids The fusion genes (heavy and/or light chain antibody fusion genes) comprising a mAb CCR5 gene segment, an optional linker gene segment and an antifusogenic peptide gene segment have been assembled with known recombinant methods and techniques by connection of the according nucleic acid segments. The nucleic acid 10 sequences encoding the peptidic linkers and antifusogenic polypeptides were each synthesized by chemical synthesis and then ligated into an E.coli plasmid for amplification. The subcloned nucleic acid sequences were verified by DNA sequencing. Example 3 15 Transient expression of immunoglobulins and immunoglobulin variants in HEK293 EBNA cells Recombinant anti-CCR5 antibodies and anti-CCR5 antibody-variants were generated by transient transfection of adherent growing HEK293-EBNA cells (human embryonic kidney cell line 293 expressing Epstein-Barr-Virus nuclear 20 antigen; American type culture collection deposit number ATCC # CRL-10852) cultivated in DMEM (Dulbecco's modified Eagle's medium, Gibco) supplemented with 10% ultra-low IgG FCS (fetal calf serum, Gibco), 2 mM Glutamine (Gibco), 1% volume by volume (v/v) nonessential amino acids (Gibco) and 250 pg/ml G418 (Roche Molecular Biochemicals). For transfection FuGENE T 6 Transfection 25 Reagent (Roche Molecular Biochemicals) was used in a ratio of reagent (pl) to DNA (pg) ranging from 3:1 to 6:1. Light and heavy chains including antifusogenic peptide-anti-CCR5 antibody conjugate light and heavy chains were expressed from two different plasmids using a molar ratio of light chain to heavy chain encoding plasmid ranging from 1:2 to 2:1, respectively. Antifusogenic peptide-anti-CCR5 30 antibody conjugates containing cell culture supernatants were harvested at day 4 to 11 after transfection. General information regarding the recombinant expression of human immunoglobulins in e.g. HEK293 cells is given in: Meissner, P. et al., Biotechnol. Bioeng. 75 (2001) 197-203.

WO 2008/019817 PCT/EP2007/007164 -41 Example 4 Expression analysis using SDS PAGE, Western blotting transfer and detection with immunoglobulin specific antibody conjugates The expressed and secreted antifusogenic peptide-anti-CCR5 antibody conjugates 5 were processed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (SDS-PAGE), and the separated anti-CCR5-antibody and antifusogenic peptide anti-CCR5-antibody-conjugate chains were transferred to a membrane from the gel and subsequently detected by an immunological method. SDS-PAGE: 10 LDS sample buffer, fourfold concentrate (4x): 4 g glycerol, 0.682 g TRIS-Base, 0.666 g TRIS-hydrochloride, 0.8 g LDS (lithium dodecyl sulfate), 0.006 g EDTA (ethylene diamin tetra acid), 0.75 ml of a 1% by weight (w/w) solution of Serva Blue G250 in water, 0.75 ml of a 1% by weight (w/w) solution of phenol red, add water to make a total volume of 10 ml. 15 The culture broth containing the secreted antifusogenic peptide-anti-CCR5 antibody conjugate was centrifuged to remove cells and cell debris. An aliquot of the clarified supernatant was admixed with 1/4 volumes (v/v) of 4xLDS sample buffer and 1/10 volume (v/v) of 0.5 M 1,4-dithiotreitol (DTT). Then the samples were incubated for 10 min. at 70 0 C and protein separated by SDS-PAGE. The 20 NuPAGE® Pre-Cast gel system (Invitrogen) was used according to the manufacturer's instruction. In particular, 10% NuPAGE® Novex® Bis-TRIS Pre Cast gels (pH 6.4) and a NuPAGE® MOPS running buffer was used. Western blot: Transfer buffer: 39 mM glycine, 48 mM TRIS-hydrochloride, 0.04% by weight 25 (w/w) SDS, and 20% by volume methanol (v/v). After SDS-PAGE the separated antifusogenic peptide-anti-CCR5 antibody conjugate chains were transferred electrophoretically to a nitrocellulose filter membrane (pore size: 0.45 pm) according to the ,,Semidry-Blotting-Method" of Burnette (Burnette, W.N., Anal. Biochem. 112 (1981) 195-203). 30 Immunological detection: TBS-buffer: 50 mM TRIS-hydrochloride, 150 mM NaC1, adjusted to pH 7.5 Blocking solution: 1% (w/v) Western Blocking Reagent (Roche Molecular Biochemicals) in TBS-buffer WO 2008/019817 PCT/EP2007/007164 - 42 TBST-Buffer: lx TBS-buffer with 0.05% by volume (v/v) Tween-20 For immunological detection the western blotting membranes were incubated with shaking at room temperature two times for 5 minutes in TBS-buffer and once for 90 minutes in blocking solution. 5 Detection of the peptide immunoglobulin conjugate chains: Heavy chain: For detection of the heavy chain of the antifusogenic peptide-anti CCR5 antibody conjugate a purified rabbit anti-human IgG antibody conjugated to a peroxidase was used (DAKO, Code No. P 0214). Light chain: The light chain of the antifusogenic peptide-anti-CCR5 antibody 10 conjugate was detected with a purified peroxidase conjugated rabbit anti-human kappa light chain antibody (DAKO, Code No. P 0129). For visualization of the antibody light and heavy chains washed and blocked Western blot membranes were first incubated in case of a heavy chain with a purified rabbit anti-human IgG antibody conjugated to a peroxidase or in case of a 15 light chain with a purified peroxidase conjugated rabbit anti-human kappa light chain antibody in a 1:10,000 dilution in 10 ml blocking solution at 4oC with shaking over night. After washing the membranes three times with TBTS-buffer and once with TBS buffer for 10 min. at room temperature, the Western-blot membranes were developed with a Luminol/peroxid-solution generating chemiluminescence 20 (Lumi-LightPLUs Western Blotting Substrate, Roche Molecular Biochemicals). Therefore the membranes were incubated in 10 ml Luminol/peroxid-solution for 10 seconds to 5 minutes and the emitted light was detected afterwards with a LUMI-Imager Fl Analysator (Roche Molecular Biochemicals) and/or was recorded with an x-ray-film. The intensity of the spots was quantified with the LumiAnalyst 25 Software (Version 3.1). Multiple-staining of immunoblots: The secondary peroxidase-labeled antibody conjugate used for the detection can be removed from the stained blot by incubating the membrane for one hour at 70 0 C in 1 M TRIS-hydrochloride-buffer (pH 6.7) containing 100 mM beta 30 mercaptoethanol and 20% (w/v) SDS. After this treatment the blot can be stained with a different secondary antibody a second time. Prior to the second detection the blot is washed three times at room temperature with shaking in TBS-buffer for 10 minutes each.

WO 2008/019817 PCT/EP2007/007164 - 43 Example 5 Affinity purification, dialysis and concentration of peptide immunoglobulin conjugates The expressed and secreted antifusogenic peptide-anti-CCR5 antibody conjugates 5 were purified by affinity chromatography using Protein A-Sepharose CL-4B (GE Healthcare former Amersham Bioscience, Sweden) according to known methods. Briefly, after centrifugation (10,000 g for 10 minutes) and filtration through a 0.45 pm filter the peptide immunoglobulin conjugate containing clarified culture supernatants were applied on a Protein A-Sepharose T M CL-4B column equilibrated 10 with PBS buffer (10 mM Na 2

HPO

4 , 1 mM KH 2

PO

4 , 137 mM NaCl and 2.7 mM KC1, pH 7.4). Unbound proteins were washed out with PBS equilibration buffer and 0.1 M citrate buffer, pH 5.5. The antifusogenic peptide-anti-CCR5 antibody conjugates were eluted with 0.1 M citrate buffer, pH 3.0, and the conjugate containing fractions were neutralized with 1 M TRIS-Base. Then, the antifusogenic 15 peptide-anti-CCR5 antibody conjugates were extensively dialyzed against PBS buffer at 4 0 C, concentrated with a Ultrafree®-CL Centrifugal Filter Unit equipped with a Biomax-SK membrane (Millipore Corp., USA) and stored in an ice-water bath at 0 0 C. The integrity of the conjugates was analyzed by SDS-PAGE in the presence and absence of a reducing agent and staining with Coomassie brilliant 20 blue as described in example 4. Aggregation of antifusogenic peptide-anti-CCR5 antibody conjugates was analyzed by analytical size exclusion chromatography. Example 6 Deglycosylation of peptide immunoglobulin conjugates N-linked carbohydrates of anti-CCR5 antibodies and antifusogenic peptide-anti 25 CCR5 antibody conjugates were cleaved off by enzymatic treatment with Peptide N-Glycosidase F (PNGaseF, Roche Molecular Biochemicals, Mannheim, Germany or Prozyme, San Leandro, CA). Therefore, the anti-CCR5 antibodies and antifusogenic peptide-anti-CCR5 antibody conjugates were incubated at 37 0 C for 12-24 h using 50 mU PNGaseF per mg N-glycosylated protein in PBS buffer at a 30 protein concentration of about 2 mg/ml. Thereafter the Peptide-N-Glycosidase F was separated by preparative gel filtration according to known methods. Briefly, PNGaseF treated anti-CCR5-antibodies and antifusogenic peptide-anti-CCR5 antibody conjugates were applied on a Superose T M 12 10/300 GL column (GE Healthcare former Amersham Bioscience, Sweden) equilibrated with PBS buffer (10 35 mM Na 2

HPO

4 , 1 mM KH 2

PO

4 , 137 mM NaCl and 2.7 mM KC1, pH 7.4) and then WO 2008/019817 PCT/EP2007/007164 - 44 eluted with equilibration buffer at a flow rate of 0.5-1.0 ml/min using the Akta explorer chromatography system from Amersham Bioscience (GE Healthcare former Amersham Bioscience, Sweden). Example 7 5 Single-cycle antiviral activity assay For the production of pseudotyped NL-Bal viruses, plasmid pNL4-3Aenv (HIV pNL4-3 genomic construct with a deletion within the env gene) and pCDNA3.1/NL-BAL env [pcDNA3.1 plasmid containing NL-Bal env gene (obtained from NIBSC Centralized Facility for AIDS Reagents)] were co 10 transfected into the HEK 293FT cell line (Invitrogen), cultured in Dulbecco's' modified minimum medium (DMEM) containing 10% fetal calf serum (FCS), 100 U/mL Penicillin, 100 .tg/mL Streptomycin, 2 mM L-glutamine and 0.5 mg/mL geniticin (all media from Invitrogen/Gibco). The supernatants containing pseudotyped viruses were harvested two days following transfection, and cellular 15 debris was removed by filtration through a 0.45 gm pore size PES (polyethersulfone) filter (Nalgene) and stored at -80 0 C in aliquots. For normalization in assay performance, virus stock aliquots were used to infect JC53 BL (US NIH Aids Reagent Program) cells yielding approximately 1.5 x 105 RLU (relative light units) per well. Test antifusogenic peptide-anti-CCR5 antibody 20 conjugates, reference antibodies and reference antifusogenic peptides (T-20, T 1249, T-651 and T-2635) were serially diluted in 96-well plates. The assay was carried out in quadruplicates. Each plate contained cell control and virus control wells. The equivalent of 1.5 x 105 RLU of virus stocks were added to each.well, then 2.5 x 104 JC53-BL cells were added to each well, with a final assay volume of 200 pl 25 per well. After 3 day incubation at 37 0 C, 90% Relative Humidity, and 5% CO 2 , media were aspirated and 50 ptl of Steady-Glo@® Luciferase Assay System (Promega) was added to each well. The assay plates were read on a Luminometer (Luminoskan, Thermo Electron Corporation) after 10 minutes of incubation at room temperature. Percent inhibition of luciferase activity was calculated for each 30 dose point after subtracting the background, and IC 5 0 and IC 90 -values were determined by using XLfit curve fitting software for Excel (version 3.0.5 Buildl2; Microsoft). Results are shown in Table 3.

WO 2008/019817 PCT/EP2007/007164 - 45 Table 3: Antiviral activity of antifusogenic polypeptides, antibodies and antifusogenic peptide-anti-CCR5 antibody conjugates Antiviral activity Compound IC 50 (ng/mL) IC 90 (ng/mL) Reference antibody 1 (inert) inactive inactive Reference antibody 2 (inert) inactive inactive T-20 206 3955 T-1249 11 90 T-651 11 139 T-2635 14 161 mAb CCR5 (4901/4900) 114 2387 Chimeric peptide mAb CCR5 conjugate (4995/4900) 7 45 Example 8 5 Cell-Cell Fusion Assay At day 1, gpl60-expressing HeLa cells (2 x 10 4 cells / 50pl / well) are seeded in a white 96 microtiter plate in DMEM medium supplemented with 10% FCS and 2pg/ml doxycycline. At day 2, 100dpl of supernatant sample or antibody control per well is added in a clear 96 microtiter plate. Then 100pl containing 8x10 4 CEM-NKr 10 Luc suspension cells in medium are added and incubated 30 min. at 37oC. The HeLa cell culture medium is aspirated from the 96 well plate, 100pl from the 200pl antibody/CEM-NKr-Luc mixture is added and incubated over night at 37oC. At day 3, 100pl/well Bright-Glo T M Luciferase assay substrate (1,4-dithiothreitol and sodium dithionite; Promega Corp., USA) is added and luminescence is measured 15 after a minimum of 15 min. incubation at RT. Materials: HeLa-R5-16 cells (cell line to express HIV gpl60 upon doxycycline induction) are cultured in DMEM medium containing nutrients and 10% FCS with 400pg/ml G418 and 200pg/ml Hygromycin B. CEM.NKR-CCR5-Luc (Catalog Number: 5198, 20 a T-cell line available from NIH AIDS Research & Reference Reagent Program McKesson BioServices Corporation Germantown, MD 20874, USA). Cell Type: CEM.NKR-CCR5 (Cat. #4376) is transfected (electroporation) to express the luciferase gene under the transcriptional control of the HIV-2 LTR and propagated in RPMI 1640 containing 10% fetal bovine serum, 4 mM glutamine, 25 penicillin/streptomycin (100 U/mL Penicillin, 100 ptg/mL Streptomycin), and 0.8 mg/ml geniticin sulfate (G418). Growth Characteristics: Round lymphoid cells, morphology not very variable. Cells grow in suspension as single cells, which can form small clumps. Split 1:10 twice weekly. Special Characteristics: Express WO 2008/019817 PCT/EP2007/007164 - 46 luciferase activity after transactivation of the HIV-2 LTR. Suitable for infection with primary HIV isolates, for neutralization and drug-sensitivity assays (Spenlehauer, C., et al., Virology 280 (2001) 292-300; Trkola, A., et al., J. Virol. 73 (1999) 8966 8974). The cell line was obtained through the NIH AIDS Research and Reference 5 Reagent Program, NIAID, NIH from Drs. John Moore and Catherine Spenlehauer. Bright-Glo T M Luciferase assay buffer (Promega Corp. USA, Part No E2264B), Bright-Glo T M , Luciferase assay substrate (Promega Corp. USA, part No EE26B). Example 9 Antiviral activity assay in peripheral blood mononuclear cells (PBMC). 10 Human PBMC are isolated from buffy-coats (obtained from the Stanford Blood Center) by a Ficoll-Paque (Amersham, Piscataway, New Jersey, USA) density gradient centrifugation according to manufacturer's protocol. Briefly, blood is transferred from the buffy coats in 50 ml conical tubes and diluted with sterile Dulbecco's phosphate buffered saline (Invitrogen/Gibco) to a final volume of 15 50 ml. Twenty-five ml of the diluted blood are transferred to two 50 ml conical tubes, carefully underlayerd with 12.5 ml of Ficoll-Paque Plus (Amersham Biosciences) and centrifuged at room temperature for 20 min. at 450 x g without braking. The white cell layer is carefully transferred to a new 50 ml conical tube and washed twice with PBS. To remove remaining red blood cells, cells are incubated 20 for 5 min. at room temperature with ACK lysis buffer (Biosource) and washed one more time with PBS. PBMC are counted and incubated at a concentration of 2 4 x 106 cells/ml in RPMIl640 containing 10% FCS (Invitrogen/Gibco), 1% penicillin/streptomycin, 2 mM L-glutamine, 1 mM sodium-pyruvate, and 2 gg/ml Phytohemagglutinin (Invitrogen) for 24h at 37 0 C. Cells are incubated with 5 25 Units/ml human IL-2 (Roche Molecular Biochemicals) for a minimum of 48h prior to the assay. In a 96 well round bottom plate, 1 x 105 PBMC are infected with the HIV-1 JR-CSF virus (Koyanagi, Y., et al., Science 236 (1987) 819-822) in the presence of serially diluted test peptide-immunoglobulin-conjugates, reference immunoglobulins and reference peptides (T-20, T-1249, T-651 and T-2635). The 30 amount of virus used is equivalent to 1.2 ng HIV-1 p24 antigen/well. Infections are set up in quadruplicates. Plates are incubated for 6 days at 37 0 C. Virus production is measured at the end of infection by using p24 ELISA (HIV-1 p24 ELISA #NEKO50B, Perkin Elmer/NEN) using the sigmoid dose-response model with one binding site in Microsoft Excel Fit (version 3.0.5 Build 12; equation 205; 35 Microsoft).

WO 2008/019817 PCT/EP2007/007164 - 47 Example 10 Making of the anti-CD4 antibody expression plasmids The gene segments encoding an anti-CD4 antibody light chain variable domain (VL) and the human kappa-light chain constant domain (CL) were joined as were 5 gene segments for the anti-CD4 antibody heavy chain variable domain (VH) and the human yl-heavy chain constant domains (CH1-Hinge-CH2-CH3). In the case of mAb CD4 of SEQ ID NO:71/72 the heavy and light chain variable domains are derived from a mouse antibody which were humanized as described e.g. by Reimann, K.A., et al., Aids Res. Human Retrovir. 13 (1997) 933-943 or in US 10 5,871,732, and the heavy and light chain constant domains are derived from a human antibody (C-kappa and IgG1). Subsequently, the gene segment encoding a complete anti-CD4 antibody light chain was joined at the N- and/or C-terminus with a nucleic acid encoding an antifusogenic peptide including a connecting linker sequence and/or the gene 15 segment encoding a complete anti-CD4 antibody heavy chain was joined at the N and/or C-terminus with a nucleic acid encoding an antifusogenic peptide including a connecting linker sequence. a) Vector 6310 Vector 6310 is an expression plasmid e.g. for transient expression of a mAb CD4 20 light chain (genomically organized expression cassette; exon-intron organization) in HEK293 cells. Beside the mAb CD4 K-light chain expression cassette this vector contains: - a neomycine resistance gene as a selectable marker, - an origin of replication from the vector pUC18 which allows replication of this 25 plasmid in E. coli, and - a 8-lactamase gene which confers ampicillin resistance in E. coli. The transcription unit of the mAb CD4 K-light chain gene is composed of the following elements: 30 - the immediate early enhancer and promoter from the human cytomegalovirus, - a 5'-untranslated region of a human antibody germline gene, - a murine immunoglobulin heavy chain signal sequence including a signal sequence intron (signal sequence 1, intron, signal sequence 2 [Ll-intron-L2]), - the humanized anti-CD4 antibody mature variable K-light chain encoding WO 2008/019817 PCT/EP2007/007164 - 48 segment arranged with a splice donor site and a unique BamHI restriction site at the 3'-end, - a truncated human K-light chain intron 2, - the human K-light gene constant domain, 5 - the bovine growth hormone (bGH) polyadenylation ("poly A") signal sequence, and - the unique restriction sites AscI and SgrAI at the 3'-end. The plasmid map of the mAb CD4 K-light chain expression vector 6310 is shown in 10 Figure 4. The amino acid sequence of the mature (without signal sequence) mAb CD4 K-light chain is shown in SEQ ID NO: 71. b) Vector 6309 Vector 6309 is an expression plasmid e.g. for transient expression of a mAb CD4 yl-heavy chain (genomically organized expression cassette; exon-intron 15 organization) in HEK293 cells. Beside the mAb CD4 yl-heavy chain expression cassette this vector contains: - an origin of replication from the vector pUC18 which allows replication of this plasmid in E. coli, and - a beta-lactamase gene which confers ampicillin resistance in E. coli. 20 The transcription unit of the mAb CD4 yl-heavy chain is composed of the following elements: - the immediate early enhancer and promoter from the human cytomegalovirus, - a 5'-untranslated region of a human antibody germline gene, 25 - a murine immunoglobulin heavy chain signal sequence including a signal sequence intron (signal sequence 1, intron, signal sequence 2 [L1-intron-L2]), - the humanized anti-CD4 antibody mature variable heavy chain encoding segment arranged with a splice donor site and a unique XhoI restriction site at the 3'-end, 30 - a mouse/human heavy chain hybrid intron 2 - the genomic human yl-heavy gene constant domains containing the L234A and L235A mutation, - the bovine growth hormone (bGH) polyadenylation ("poly A") signal sequence, and 35 - the unique restriction site SgrAI at the 3'-end.

WO 2008/019817 PCT/EP2007/007164 - 49 The plasmid map of the mAb CD4 yl -heavy chain expression vector 6309 is shown in Figure 3 The amino acid sequence of the mature (without signal sequence) mAb CD4 yl-heavy chain is shown in SEQ ID NO: 72. c) Vector 6303 5 Vector 6303 is an expression plasmid e.g. for transient expression of a chimeric peptide-anti-CD4 antibody yl-heavy chain conjugate (genomically organized expression cassette; exon-intron organization) in HEK293 cells. The vector 6303 is derived from vector 6309 in that way that the mAb CD4 y1 heavy chain is joint at the last but one C-terminal amino acid, i.e. the C-terminal 10 lysine residue of the heavy chain is removed, with a nucleic acid encoding the antifusogenic peptide afp-1 (SEQ ID NO:73) and the peptidic glycin-serine linker of SEQ ID NO:69. Beside the chimeric peptide anti-CD4 antibody yl-heavy chain conjugate expression cassette this vector contains: 15 - an origin of replication from the vector pUC18 which allows replication of this plasmid in E. coli, and - a beta(p)-lactamase gene which confers ampicillin resistance in E. coli. The transcription unit of the chimeric peptide-anti-CD4 antibody yl-heavy chain 20 conjugate is composed of the following elements: - the immediate early enhancer and promoter from the human cytomegalovirus, - a 5'-untranslated region of a human antibody germline gene, - a murine immunoglobulin heavy chain signal sequence including a signal sequence intron (signal sequence 1, intron, signal sequence 2 [L1-intron-L2]), 25 - the humanized anti-CD4 antibody mature variable heavy chain encoding segment arranged with a splice donor site and a unique XhoI restriction site at the 3'-end, - a mouse/human heavy chain hybrid intron containing the L234A and L235A mutations, 30 - the peptidic serine-glycin linker sequence of SEQ ID NO:69, - the antifusogenic peptide afp-1 of SEQ ID NO:73, - the bovine growth hormone (bGH) polyadenylation ("poly A") signal sequence, and - the unique restriction site SgrAI at the 3'-end. 35 WO 2008/019817 PCT/EP2007/007164 - 50 The plasmid map of the mAb CD4 yl-heavy chain conjugate expression vector 6303 is shown in Figure 6. The amino acid sequence of the mature (without signal sequence) conjugate heavy chain is shown in SEQ ID NO:74. Example 11 5 Making of the final anti-CD4 antibody expression plasmids The fusion genes (heavy and/or light chain antibody fusion genes) comprising a mAb CD4 gene segment, an optional linker gene segment and an antifusogenic peptide gene segment have been assembled with known recombinant methods and techniques by connection of the according nucleic acid segments. The nucleic acid 10 sequences encoding the peptidic linkers and antifusogenic polypeptides were each synthesized by chemical synthesis and then ligated into an E.coli plasmid for amplification. The subcloned nucleic acid sequences were verified by DNA sequencing. Example 12 15 Transient expression of immunoglobulins and immunoglobulin variants in HEK293 EBNA cells Recombinant anti-CD4 antibodies and anti-CD4 antibody-variants were generated by transient transfection of adherent growing HEK293-EBNA cells (human embryonic kidney cell line 293 expressing Epstein-Barr-Virus nuclear antigen; 20 American type culture collection deposit number ATCC # CRL-10852) cultivated in DMEM (Dulbecco's modified Eagle's medium, Gibco) supplemented with 10% ultra-low IgG FCS (fetal calf serum, Gibco), 2 mM Glutamine (Gibco), 1% volume by volume (v/v) nonessential amino acids (Gibco) and 250 [tg/ml G418 (Roche Molecular Biochemicals). For transfection FuGENE T 6 Transfection Reagent 25 (Roche Molecular Biochemicals) was used in a ratio of reagent ([l) to DNA (pg) ranging from 3:1 to 6:1. Light and heavy chains including antifusogenic peptide anti-CD4 antibody conjugate light and heavy chains were expressed from two different plasmids using a molar ratio of light chain to heavy chain encoding plasmid ranging from 1:2 to 2:1, respectively. Antifusogenic peptide-anti-CD4 30 antibody conjugates containing cell culture supernatants were harvested at day 4 to 11 after transfection. General information regarding the recombinant expression of human immunoglobulins in e.g. HEK293 cells is given in: Meissner, P. et al., Biotechnol. Bioeng. 75 (2001) 197-203.

WO 2008/019817 PCT/EP2007/007164 -51 Example 13 Expression analysis using SDS PAGE, Western blotting transfer and detection with immunoglobulin specific antibody conjugates The expressed and secreted antifusogenic peptide-anti-CD4 antibody conjugates 5 were processed by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (SDS-PAGE), and the separated anti-CD4-antibody and antifusogenic peptide anti-CD4-antibody-conjugate chains were transferred to a membrane from the gel and subsequently detected by an immunological method. SDS-PAGE: 10 LDS sample buffer, fourfold concentrate (4x): 4 g glycerol, 0.682 g TRIS-Base, 0.666 g TRIS-hydrochloride, 0.8 g LDS (lithium dodecyl sulfate), 0.006 g EDTA (ethylene diamin tetra acid), 0.75 ml of a 1% by weight (w/w) solution of Serva Blue G250 in water, 0.75 ml of a 1% by weight (w/w) solution of phenol red, add water to make a total volume of 10 ml. 15 The culture broth containing the secreted antifusogenic peptide-anti-CD4 antibody conjugate was centrifuged to remove cells and cell debris. An aliquot of the clarified supernatant was admixed with 1/4 volumes (v/v) of 4xLDS sample buffer and 1/10 volume (v/v) of 0.5 M 1,4-dithiotreitol (DTT). Then the samples were incubated for 10 min. at 70'C and protein separated by SDS-PAGE. The NuPAGE® Pre-Cast 20 gel system (Invitrogen) was used according to the manufacturer's instruction. In particular, 10% NuPAGE® Novex® Bis-TRIS Pre-Cast gels (pH 6.4) and a NuPAGE® MOPS running buffer was used. Western blot: Transfer buffer: 39 mM glycine, 48 mM TRIS-hydrochloride, 0.04 % by weight 25 (w/w) SDS, and 20 % by volume methanol (v/v). After SDS-PAGE the separated antifusogenic peptide-anti-CD4 antibody conjugate chains were transferred electrophoretically to a nitrocellulose filter membrane (pore size: 0.45 pm) according to the ,,Semidry-Blotting-Method" of Burnette (Burnette, W.N., Anal. Biochem. 112 (1981) 195-203). 30 Immunological detection: TBS-buffer: 50 mM TRIS-hydrochloride, 150 mM NaC1, adjusted to pH 7.5 Blocking solution: 1% (w/v) Western Blocking Reagent (Roche Molecular Biochemicals) in TBS-buffer WO 2008/019817 PCT/EP2007/007164 - 52 TBST-Buffer: lx TBS-buffer with 0.05 % by volume (v/v) Tween-20 For immunological detection the western blotting membranes were incubated with shaking at room temperature two times for 5 minutes in TBS-buffer and once for 90 minutes in blocking solution. 5 Detection of the peptide immunoglobulin conjugate chains: Heavy chain: For detection of the heavy chain of the antifusogenic peptide-anti CD4 antibody conjugate a purified rabbit anti-human IgG antibody conjugated to a peroxidase was used (DAKO, Code No. P 0214). Light chain: The light chain of the antifusogenic peptide-anti-CD4 antibody 10 conjugate was detected with a purified peroxidase conjugated rabbit anti-human kappa light chain antibody (DAKO, Code No. P 0129). For visualization of the antibody light and heavy chains washed and blocked Western blot membranes were first incubated in case of a heavy chain with a purified rabbit anti-human IgG antibody conjugated to a peroxidase or in case of a 15 light chain with a purified peroxidase conjugated rabbit anti-human kappa light chain antibody in a 1:10,000 dilution in 10 ml blocking solution at 4oC with shaking over night. After washing the membranes three times with TBTS-buffer and once with TBS buffer for 10 min. at room temperature, the Western-blot membranes were developed with a Luminol/peroxid-solution generating chemiluminescence 20 (Lumi-LightPLUs Western Blotting Substrate, Roche Molecular Biochemicals). Therefore the membranes were incubated in 10 ml Luminol/peroxid-solution for 10 seconds to 5 minutes and the emitted light was detected afterwards with a LUMI-Imager F1 Analysator (Roche Molecular Biochemicals) and/or was recorded with an x-ray-film. The intensity of the spots was quantified with the LumiAnalyst 25 Software (Version 3.1). Multiple-staining of immunoblots: The secondary peroxidase-labeled antibody conjugate used for the detection can be removed from the stained blot by incubating the membrane for one hour at 70 0 C in 1 M TRIS-hydrochloride-buffer (pH 6.7) containing 100 mM beta 30 mercaptoethanol and 20 % (w/v) SDS. After this treatment the blot can be stained with a different secondary antibody a second time. Prior to the second detection the blot is washed three times at room temperature with shaking in TBS-buffer for 10 minutes each.

WO 2008/019817 PCT/EP2007/007164 - 53 Example 14 Affinity purification, dialysis and concentration of peptide immunoglobulin conjugates The expressed and secreted antifusogenic peptide-anti-CD4 antibody conjugates 5 were purified by affinity chromatography using Protein A-Sepharose CL-4B (GE Healthcare former Amersham Bioscience, Sweden) according to known methods. Briefly, after centrifugation (10,000 g for 10 minutes) and filtration through a 0.45 pm filter the peptide immunoglobulin conjugate containing clarified culture supernatants were applied on a Protein A-Sepharose T M CL-4B column equilibrated 10 with PBS buffer (10 mM Na 2

HPO

4 , 1 mM KH 2

PO

4 , 137 mM NaCl and 2.7 mM KC1, pH 7.4). Unbound proteins were washed out with PBS equilibration buffer and 0.1 M citrate buffer, pH 5.5. The antifusogenic peptide-anti-CD4 antibody conjugates were eluted with 0.1 M citrate buffer, pH 3.0, and the conjugate containing fractions were neutralized with 1 M TRIS-Base. Then, the antifusogenic 15 peptide-anti-CD4 antibody conjugates were extensively dialyzed against PBS buffer at 4 0 C, concentrated with a Ultrafree®-CL Centrifugal Filter Unit equipped with a Biomax-SK membrane (Millipore Corp., USA) and stored in an ice-water bath at 0OC. The integrity of the conjugates was analyzed by SDS-PAGE in the presence and absence of a reducing agent and staining with Coomassie brilliant blue as described 20 in Example 13. Aggregation of antifusogenic peptide-anti-CD4 antibody conjugates was analyzed by analytical size exclusion chromatography. Example 15 Deglycosylation of peptide immunoglobulin conjugates N-linked carbohydrates of anti-CD4 antibodies and antifusogenic peptide-anti 25 CD4 antibody conjugates were cleaved off by enzymatic treatment with Peptide-N Glycosidase F (PNGaseF, Roche Molecular Biochemicals, Mannheim, Germany or Prozyme, San Leandro, CA). Therefore, the anti-CD4 antibodies and antifusogenic peptide-anti-CD4 antibody conjugates were incubated at 37 0 C for 12-24 h using 50 mU PNGaseF per mg N-glycosylated protein in PBS buffer at a protein 30 concentration of about 2 mg/ml. Thereafter the Peptide-N-Glycosidase F was separated by preparative gel filtration according to known methods. Briefly, PNGaseF treated anti-CD4-antibodies and antifusogenic peptide-anti-CD4 antibody conjugates were applied on a Superose T M 12 10/300 GL column (GE Healthcare former Amersham Bioscience, Sweden) equilibrated with PBS buffer 35 (10 mM Na 2

HPO

4 , 1 mM KH 2

PO

4 , 137 mM NaCl and 2.7 mM KCI, pH 7.4) and WO 2008/019817 PCT/EP2007/007164 - 54 then eluted with equilibration buffer at a flow rate of 0.5-1.0 ml/min using the Akta explorer chromatography system from Amersham Bioscience (GE Healthcare former Amersham Bioscience, Sweden). Example 16 5 Single-cycle antiviral activity assay. For the production of pseudotyped NL-Bal viruses, plasmid pNL4-3Aenv (HIV pNL4-3 genomic construct with a deletion within the env gene) and pCDNA3.1/NL-BAL env [pcDNA3.1 plasmid containing NL-Bal env gene (obtained from NIBSC Centralized Facility for AIDS Reagents)] were co 10 transfected into the HEK 293FT cell line (Invitrogen Corp.), cultured in Dulbecco's' modified minimum medium (DMEM) containing 10% fetal calf serum (FCS), 100 U/mL Penicillin, 100 pg/mL Streptomycin, 2 mM L-glutamine and 0.5 mg/mL geniticin (all media from Invitrogen/Gibco). The supernatants containing pseudotyped viruses were harvested two days following transfection, and cellular 15 debris was removed by filtration through a 0.45 tm pore size PES (polyethersulfone) filter (Nalgene) and stored at -80oC in aliquots. For normalization in assay performance, virus stock aliquots were used to infect JC53 BL (US NIH Aids Reagent Program) cells yielding approximately 1.5 x 10 s RLU (relative light units) per well. Test antifusogenic peptide-anti-CD4 antibody 20 conjugates, parent anti-CD4 antibody, reference antibodies and reference antifusogenic peptide (T-651) were serially diluted in 96-well plates. The assay was carried out in quadruplicates. Each plate contained cell control and virus control wells. The equivalent of 1.5 x 105 RLU of virus stocks were added to each well, then 2.5 x 104 JC53-BL cells were added to each well, with a final assay volume of 200 gl 25 per well. After 3 day incubation at 37 0 C, 90 % Relative Humidity, and 5 % CO 2 , media were aspirated and 50 p1 of Steady-Glo@® Luciferase Assay System (Promega) was added to each well. The assay plates were read on a Luminometer (Luminoskan, Thermo Electron Corporation) after 10 minutes of incubation at room temperature. Percent inhibition of luciferase activity was calculated for each 30 dose point after subtracting the background, and IC 50 and IC 90 -values were determined by using XLfit curve fitting software for Excel (version 3.0.5 Buildl2; Microsoft). Results are shown in Table 4 and 5, respectively.

WO 2008/019817 PCT/EP2007/007164 - 55 Table 4: Antiviral activity of antifusogenic polypeptides, antibodies and antifusogenic peptide-anti-CD4 antibody conjugates (by weight). Antiviral activity Compound NL-BAL (R5) NL-4-3 (X4)

IC

5 0 [ng/mL] / IC 90

IC

50 [ng/mL] / ICo 90 [ng/mL] [ng/mL] Reference antibody 1 (inert) inactive / inactive inactive / inactive Reference antibody 2 (inert) inactive / inactive inactive / inactive T-651 21.5 / 181.9 169.2 / 1099.7 mAb CD4 (6310/6309) maximal inhibition Maximal inhibition = 50% = 20 % antifusogenic peptide-anti CD4 6.132.2 43.9 / 123.9 antibody conjugate (6310/6303) Table 5: Antiviral activity of antifusogenic polypeptides, antibodies and 5 antifusogenic peptide-anti-CD4 antibody conjugates (by molarity). Antiviral activity Compound NL-BAL (R5) NL-4-3 (X4)

IC

50 [nM]/ ICo 90 [nM] IC 50 [nM] / IC 90 [nM] Reference antibody 1 (inert) inactive / inactive Inactive / inactive Reference antibody 2 (inert) inactive / inactive Inactive / inactive T-651 5.4 / 45 42 / 275 mAb CD4 (6310/6309) maximal inhibition Maximal inhibition = 50 % = 20 % antifusogenic peptide-anti CD4 0.038 / 0.201 0.275 / 0.774 antibody conjugate (6310/6303) Example 17 Cell-Cell Fusion Assay At day 1, gpl60-expressing HeLa cells (2 x 10 4 cells / 50 pl / well) are seeded in a 10 white 96 microtiter plate in DMEM medium supplemented with 10 % FCS and 2pg/ml doxycycline. At day 2, 100 pl of supernatant sample or antibody control per well is added in a clear 96 microtiter plate. Then 100 pl containing 8x10 4

CEM

NKr-Luc suspension cells in medium are added and incubated 30 min. at 37 oC. The HeLa cell culture medium is aspirated from the 96 well plate, 100 p1 from the 15 200 pl antibody/CEM-NKr-Luc mixture is added and incubated over night at 37 'C. At day 3, 100 pl/well Bright-Glo T M Luciferase assay substrate (1,4-dithiothreitol and sodium dithionite; Promega Corp., USA) is added and luminescence is measured after a minimum of 15 min. incubation at RT.

WO 2008/019817 PCT/EP2007/007164 - 56 Materials: HeLa-R5-16 cells (cell line to express HIV gpl60 upon doxycycline induction) are cultured in DMEM medium containing nutrients and 10 % FCS with 400 pg/ml G418 and 200 pg/ml Hygromycin B. CEM.NKR-CD4-Luc (Catalog Number: 5198, 5 a T-cell line available from NIH AIDS Research & Reference Reagent Program McKesson BioServices Corporation Germantown, MD 20874, USA). Cell Type: CEM.NKR-CD4 (Cat. #4376) is transfected (electroporation) to express the luciferase gene under the transcriptional control of the HIV-2 LTR and propagated in RPMI 1640 containing 10 % fetal bovine serum, 4 mM glutamine, 10 penicillin/streptomycin (100 U/mL Penicillin, 100 tg/mL Streptomycin), and 0.8 mg/ml geniticin sulfate (G418). Growth Characteristics: Round lymphoid cells, morphology not very variable. Cells grow in suspension as single cells, which can form small clumps. Split 1:10 twice weekly. Special Characteristics: Express luciferase activity after transactivation of the HIV-2 LTR. Suitable for infection with 15 primary HIV isolates, for neutralization and drug-sensitivity assays (Spenlehauer, C., et al., Virology 280 (2001) 292-300; Trkola, A., et al., J. Virol. 73 (1999) 8966 8974). The cell line was obtained through the NIH AIDS Research and Reference Reagent Program, NIAID, NIH from Drs. John Moore and Catherine Spenlehauer. Bright-Glo T M Luciferase assay buffer (Promega Corp. USA, Part No E2264B), 20 Bright-Glo T M , Luciferase assay substrate (Promega Corp. USA, part No EE26B). Example 18 Antiviral activity assay in peripheral blood mononuclear cells (PBMC). Human PBMC are isolated from buffy-coats (obtained from the Stanford Blood Center) by a Ficoll-Paque (Amersham, Piscataway, New Jersey, USA) density 25 gradient centrifugation according to manufacturer's protocol. Briefly, blood is transferred from the buffy coats in 50 ml conical tubes and diluted with sterile Dulbecco's phosphate buffered saline (Invitrogen/Gibco) to a final volume of 50 ml. Twenty-five ml of the diluted blood are transferred to two 50 ml conical tubes, carefully underlayerd with 12.5 ml of Ficoll-Paque Plus (Amersham 30 Biosciences) and centrifuged at room temperature for 20 min. at 450 x g without braking. The white cell layer is carefully transferred to a new 50 ml conical tube and washed twice with PBS. To remove remaining red blood cells, cells are incubated for 5 min. at room temperature with ACK lysis buffer (Biosource) and washed one more time with PBS. PBMC are counted and incubated at a concentration of 2 35 4 x 106 cells/ml in RPMI1640 containing 10% FCS (Invitrogen/Gibco), 1% penicillin/streptomycin, 2 mM L-glutamine, 1 mM sodium-pyruvate, and 2 gtg/ml WO 2008/019817 PCT/EP2007/007164 - 57 Phytohemagglutinin (Invitrogen) for 24 h at 37 oC. Cells are incubated with 5 Units/ml human IL-2 (Roche Molecular Biochemicals) for a minimum of 48 h prior to the assay. In a 96 well round bottom plate, 1 x 10 s PBMC are infected with the HIV-1 JR-CSF virus (Koyanagi, Y., et al., Science 236 (1987) 819-822) in the 5 presence of serially diluted test antifusogenic peptide-anti-CD4 antibody conjugates, reference immunoglobulins, parent ant-CD4 antibody, and reference peptides (T-651). The amount of virus used is equivalent to 1.2 ng HIV-1 p24 antigen/well. Infections are set up in quadruplicates. Plates are incubated for 6 days at 37 oC. Virus production is measured at the end of infection by using p24 ELISA 10 (HIV-1 p24 ELISA #NEKO50B, Perkin Elmer/NEN) using the sigmoid dose response model with one binding site in Microsoft Excel Fit (version 3.0.5 Build 12; equation 205; Microsoft). Table 6: Antiviral activity of antifusogenic polypeptides, antibodies and antifusogenic peptide-anti-CD4 antibody conjugates (by weight). Antiviral activity NL-BAL NL-4-3 Compound IC 50 [ng/mL] / IC 90

IC

50 [ng/mL] / ICo 9 0 [ng/mL] [ng/mL] Reference antibody 1 (inert) inactive / inactive inactive / inactive Reference antibody 2 (inert) inactive / inactive inactive / inactive T-651 14.1 / 57.8 41.4 / 79.6 anti-CD4 antibody (6310/6309) 917.6 / max. 54% 35.6 / 1117.0 inhibition antifusogenic peptide-anti CD4 5.318.2 3.225.1 antibody conjugate (6310/6303) 15

Claims

1. A conjugate comprising one or more antifusogenic peptides and an antibody against an HIV gpl20 binding cell surface receptor, characterized in that one to eight antifusogenic peptides are each conjugated to one terminus of the 5 heavy and/or light chains of said antibody against an HIV gpl20 binding cell surface receptor.

2. A conjugate according to claim 1, characterized in that the antibody against an HIV gpl20 binding cell surface receptor is conjugated to two or four antifusogenic peptides. 10

3. A conjugate according to claim 1, characterized in that the antibody against an HIV gpl20 binding cell surface receptor is an anti-CCR5 antibody (mAb CCR5).

4. A conjugate according to claim 3, characterized in that at least two of the termini of said anti-CCR5 antibody are each conjugated to an antifusogenic 15 peptide.

5. A conjugate according to claim 3, characterized in that said antifusogenic peptides are linear peptides and comprise an amino acid sequence of from 5 to 100 amino acids.

6. A conjugate according to any one of claims 3 to 5, characterized by the 20 general formula mAb CCR5 - [linker]m - [antifusogenic peptide]n, wherein m is independently for each antifusogenic peptide either 0 or 1, and n is an integer of from at most 1 to 8.

7. A conjugate according to claim 6, characterized in comprising a conjugate of heavy and/or light chain of mAb CCR5 and antifusogenic peptide(s) ("chain 25 conjugate"), selected from the group consisting of: (1) [antifusogenic peptide] - [linker]m - [heavy chain] (2) [heavy chain] - [linker]m - [antifusogenic peptide] (3) [antifusogenic peptide] - [linker]m - [heavy chain] - [antifusogenic peptide] 30 (4) [antifusogenic peptide] - [linker]m - [light chain] (5) [light chain] - [linker]m - [antifusogenic peptide] WO 2008/019817 PCT/EP2007/007164 - 59 (6) [antifusogenic peptide] - [linker]m - [light chain] - [antifusogenic peptide] (7) [antifusogenic peptide] - [linker]m - [heavy chain] - [linker]m [antifusogenic peptide] 5 (8) [antifusogenic peptide] - [linker]m - [light chain] - [linker]m [antifusogenic peptide] wherein the linker can be the same or different, wherein m is an integer of 1 or 0 and m can be independently the same or different. 10

8. A conjugate according to claim 7, characterized in comprising a chain conjugate (2), (3), (4), or (7).

9. A conjugate according to claim 7, characterized in comprising 2x [mAb CCR5 light chain] and 2x (2), 2 x [mAb CCR5 light chain] and 2 x (3), 2 x [mAb CCR5 heavy chain] and 2 x (4), or 2 x [mAb CCR5 light chain] and 2 x (7). 15

10. A conjugate according to any one of claims 3 to 9, characterized in that said antifusogenic peptide is selected from the group of peptides defined by SEQ ID NO:29 to 35 and SEQ ID NO:73.

11. A conjugate according to any one of claims 3 to 10, characterized in that said anti-CCR5 antibody comprises a variable heavy chain domain consisting of 20 an immunoglobulin framework and a CDR3 region selected from the group consisting of the heavy chain CDR3 sequences SEQ ID NO: 16, 17.

12. A conjugate according to any one of claims 3 to 11, characterized in that said anti-CCR5 antibody comprises a variable heavy chain domain consisting of an immunoglobulin framework and a CDR3 region selected from the group 25 consisting of heavy chain CDR3 sequences SEQ ID NO:16 and 17, a CDR2 region selected from the group consisting of heavy chain CDR2 sequences SEQ ID NO:13, 14, and 15, and a CDR1 region selected from the group consisting of heavy chain CDR1 sequences SEQ ID NO:9, 10, 11, and 12.

13. A conjugate according to any one of claims 3 to 12, characterized in that said 30 anti-CCR5 antibody comprises a heavy chain variable domain selected from the group of SEQ ID NO:1, 3, 5, and 7. WO 2008/019817 PCT/EP2007/007164 - 60

14. A conjugate according to any one of claims 3 to 13, characterized in that said anti-CCR5 antibody comprises a variable light chain domain consisting of an immunoglobulin framework and a CDR1 region selected from SEQ ID NO:18, 19, and 20, a CDR2 region selected from SEQ ID NO:21, 22, and 23, 5 and a CDR3 region selected from SEQ ID NO:24, and 25.

15. A conjugate according to any one of claims 3 to 14, characterized in that said anti-CCR5 antibody comprises as heavy chain CDRs the CDRs of SEQ ID NO: 1 and as light chain CDRs the CDRs of SEQ ID NO: 2, as heavy chain CDRs the CDRs of SEQ ID NO: 3 and as light chain CDRs the CDRs of SEQ 10 ID NO: 4, as heavy chain CDRs the CDRs of SEQ ID NO: 5 and as light chain CDRs the CDRs of SEQ ID NO: 6, or as heavy chain CDRs the CDRs of SEQ ID NO: 7 and as light chain CDRs the CDRs of SEQ ID NO: 8.

16. A conjugate according to any one of claims 3 to 15, characterized in that said anti-CCR5 antibody comprises a variable heavy chain domain and a variable 15 light chain domain independently selected from the group consisting of a) the heavy chain (VH) variable domain defined by amino acid sequence SEQ ID NO: 1 and the light chain (VL) variable domain defined by SEQ ID NO:2; b) the heavy chain variable domain defined by amino acid sequence SEQ 20 ID NO:3 and the light chain variable domain defined by SEQ ID NO:4; c) the heavy chain variable domain defined by amino acid sequence SEQ ID NO:5 and the light chain variable domain defined by SEQ ID NO:6; d) the heavy chain variable domain defined by amino acid sequence SEQ ID NO:7 and the light chain variable domain defined by SEQ ID NO:8. 25

17. A conjugate according to any one of claims 3 to 16, characterized in that said anti-CCR5 antibody comprises the heavy chain (VH) variable domain defined by amino acid sequence SEQ ID NO:I1 and the light chain (VL) variable domain defined by SEQ ID NO:2, the heavy chain variable domain defined by 30 amino acid sequence SEQ ID NO:3 and the light chain variable domain defined by amino acid SEQ ID NO:4, the heavy chain variable domain defined by amino acid sequence SEQ ID NO:5 and the light chain variable domain defined by amino acid SEQ ID NO:6, or the heavy chain variable domain defined by amino acid sequence SEQ ID NO:7 and the light chain 35 variable domain defined by amino acid SEQ ID NO:8; WO 2008/019817 PCT/EP2007/007164 -61 a linker selected from the group consisting of amino acids glycine (G) and asparagine (N), the tripeptide GST, and SEQ ID NO: 36-62, and SEQ ID NO:67-70; and an antifusogenic peptide selected from the group of peptides defined by SEQ 5 ID NO:29 to 35 and SEQ ID NO:73.

18. A conjugate according to any one of claims 3 to 16, characterized in comprising an antifusogenic peptide selected from the group of SEQ ID NO:29 to 35 and SEQ ID NO:73. 10

19. A conjugate according to any one of claims 3 to 18, characterized in comprising two light chain variable domains of SEQ ID NO:2, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:1, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, in comprising two light chain variable domains of SEQ ID NO:4, two conjugates 15 of type (2) each comprising a heavy chain variable domain of SEQ ID NO:3, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, in comprising two light chain variable domains of SEQ ID NO:6, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:5, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33, or in 20 comprising two light chain variable domains of SEQ ID NO:8, two conjugates of type (2) each comprising a heavy chain variable domain of SEQ ID NO:7, a linker of SEQ ID NO:40 and an antifusogenic peptide of SEQ ID NO:33.

20. A conjugate according to any one of claims 3 to 19, characterized in that said anti-CCR5 antibody is of IgG4 subclass, or of IgG1 or IgG2 subclass, with a 25 mutation in amino acid S228, L234, L235, and/or D265, and/or contains the PVA236 mutation

21. A conjugate according to claim 20, characterized in that said anti-CCR5 antibody of IgG4 subclass has the mutation S228P and said anti-CCR5 antibody of IgG1 subclass has the mutations L234A and L235A. 30

22. A method for the production of a conjugate according to any one of claims 1 to 21, characterized in that the method comprises a) cultivating a cell containing one or more plasmids containing one or more nucleic acid molecules encoding a conjugate according to claims 1 to 21 under conditions suitable for the expression of the conjugate, WO 2008/019817 PCT/EP2007/007164 - 62 b) recovering the conjugate from the cell or the cell culture supernatant.

23. A pharmaceutical composition, containing a conjugate according to any one of claims 1 to 21, together with a pharmaceutically acceptable excipient or 5 carrier.

24. Use of a conjugate according to any one of claims 1 to 21 for the manufacture of a medicament for the treatment of viral infections.

25. Use according to claim 24, characterized in that the viral infection is a HIV infection. 10

26. Use of a conjugate according to any one of claims 1 to 21 for the treatment of a patient in need of an antiviral treatment.