AU2007216781A1 - Compounds for the inhibition of histone deacetylase - Google Patents

Description

S&FRef: 826338

AUSTRALIA

PATENTS ACT 1990 COMPLETE SPECIFICATION FOR A STANDARD PATENT Name and Address of Applicant Actual Inventor(s): Address for Service: Invention Title: Naturewise Biotech Medicals Corporation, of 3F, 216 Wenlin North Road, Peitou, Taipei, 112, Taiwan Chung-Yang Huang, Chia-Nan Chen, Wei-Jan Huang, Chih-Hsiang Huang, Li-Ling Chi, Chiou-Ping You Spruson Ferguson St Martins Tower Level 31 Market Street Sydney NSW 2000 (CCN 3710000177) Compounds for the inhibition of histone deacetylase The following statement is a full description of this invention, including the best method of performing it known to me/us:- 5845c(949352 1) COMPOUNDS FOR THE INHIBITION OF HISTONE DEACETYLASE Field of the Invention S[0001] The present invention relates to novel compounds which are useful as the agents for prevention or treatment of diseases associated with histone deacetylase (HDAC), in particular, tumor or cell proliferative diseases. They also can be used as the 00 agents for enhancing the neurite outgrowth. In particular, the compounds of the c' 10 invention can be used as agents for anti-neurodegenerative diseases and human spinal 0 muscular atrophy (SMA).

Background of the Invention [0002] Eukaryotic DNA is highly organized and packaged in the nucleus. The organization and packaging are achieved through the addition of proteins, including core histones H2A, H2B, H3 and 1-14, which form a complex structure, the chromatin, together with DNA. The modification of core histoncs is of fundamental importance to conformational changes of the chromatin. The level of acetylation is related to transcription activity, and then the acetylation induces an open chromatin conformation that allows the transcription machinery access to promoters. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that influence transcription by selectively deacetylating or acetylating the c-amino groups of lysine located near the amino termini of core histone proteins. HDAC is a family of 1 1 enzymes (isoforms) that may act as master regulators of many diseases, including cancer, because they are -1involved in the control of gene expression. Disruption of HDACs has been linked to a Swide variety of human cancers. HDAC enzymes or isoforms appear to be involved in 0 many different types of cancer.

[0003] Histone deacetylase (HDAC) inhibitors are emerging as an exciting new class of potential anticancer agents for the treatment of solid and hematological malignancies. In recent years, an increasing number of structurally diverse HDAC 00 inhibitors have been identified; they inhibit proliferation and induce differentiation and/or r apoptosis of tumor cells in culture and in animal models. HDAC inhibition causes acetylated nuclear histones to accumulate in both tumor and normal tissues, providing a surrogate marker for the biological activity of HDAC inhibitors in vivo. The effects of HDAC inhibitors on gene expression are highly selective, leading to transcriptional activation of certain genes such as the cyclin-dependent kinase inhibitor p21WAFI/CIPI but repression of others. HDAC inhibition not only results in acetylation of histones but also transcription factors such as p53, GATA-1 and estrogen receptor-alpha. The functional significance of acetylation of non-histone proteins and the precise mechanisms whereby HDAC inhibitors induce, tumor cell growth arrest, differentiation and/or apoptosis are currently the focus of intensive research. HDAC inhibitors currently in clinical trials have shown activity and represent a class of molecularly targeted antitumour agents with potential for efficacy based on a novel mechanism of action.

[0004] A review article published in Medicinal Research Reviews, Vol. 26, No. 4, pp. 397-413, 2006 stated that four classes of HDAC inhibitors, short-chain fatty acids, hydroxamic acids, benzamides and cyclic peptides, have been reported. Hydroxamic acid-based hybrid polar compounds (HPCs) are HDAC inhibitors, which induce differentiation at micromolar or lower concentrations (Journal of the National Cancer Institute, Vol. 92, No. 15, August 2, 2000, pp. 1210-1216). U.S. Pat. No. 6,174,905, EP 0847992, JP 258863/96, and Japanese Application No. 10138957 disclose benzamide derivatives that induce cell differentiation and inhibit HDAC. WO 01/38322 discloses additional compounds that serve as HDAC inhibitors. It was reported in Hum Genet, oO I 2006, 120, pp. 101-110 that the benzamide M344 up-regulates SMN2 protein expression (Ni in fibroblast cells derived from SMA patients up to 7-fold after 64 hours of treatment. It Swas reported that sodium butyrate ameliorates phenotypic expression in a transgenic mouse model of spinal and bulbar-muscular atrophy (Human Molecular Genetics, 2004, Vol. 13, No. 11, pp. 1183-1192).' Trichostatin A, a histone deacetylase inhibitor, was found to induce ubiquitin-dependent cyclin Dl degradation in MCF-7 breast cancer cells (Molecular Cancer 2006, 5:8; this article is available from: http://www.molecularcancer.com/content/5/1/8). U.S. Pat. No. 7,169,801 disclosed compounds that may be used to inhibit histone deacetylase having the formula Z-Q-L-M or Z-L-M. US Patent Number 6888027 covers a family of Sulphonamide HDAC inhibitors including PXD101.

European Patent Number EP 1 301 184 covers the use of valproic acid and derivatives as HDAC inhibitors in the treatment of solid tumors.

[0005] However, there is till, a need to develop a new class of HDAC inhibitors to prevent or treat cancers.

Summary of the Invention [0006] The object of the invention is to provide a group of compounds represented by the following formula

N(I)

IRR4 R7 and pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates I 5 thereof. The compounds are useful as an agent for enhancing the neurite outgrowth and preventing or treating diseases associated with HDAC in particular, tumor or cell proliferative diseases. In particular, the compounds of the invention can be used as agents for anti-neurodegenerative diseases and human spinal muscular atrophy (SMA).

Brief Description of the Drawings [0007] Fig. 1 shows the microscopic photographs of the rat glioma C6 cells treated with different concentrations of NBM-HD-1.

[0008] Fig. 2 shows the DNA contents of the rat glioma C6 cells analyzed by FACScan Cytometry.

[0009] Fig. 3 shows the electrophoresis plot of the genes of cell cycle of the rat glioma C6 cells treated with different doses of NBM-HD-1.

[0010] Fig. 4 shows the immuno-fluorescent staining photographs and RT-PCR data of the rat glioma C6 cells treated with NBM-HD-I.

[0011] Fig. 5 shows Western Blotting plot of the rat glioma C6 cells treated with NBM-HD-1 and sodium butyrate.

[0012] Fig. 6 shows the relative inhibited activity of the HDAC on the rat glioma C6 cells treated with NBM-HD-1 apd sodium butyrate.

[0013] Fig. 7 shows the microscopic photographs of the human giloblastoma cancer cells treated with different doses of NBM-HD-1.

00 r- \D [0014] Fig. 8 shows the microscopic photographs of the human breast MCF-7

(N-

cancer cells treated with different doses of NBM-HD-1.

[0015] Fig. 9 shows that NBM-HD-1 markedly inhibited the MCF-7 cell growth via modulation of the cell cycle arrested on the GO/G1 phase in a dose-dependent manner.

[0016] Fig. 10 shows that NBM-HD-1 markedly increased the p2 1

WAF

I

C

i

P

I gene expression in a dose-dependent manner.

[0017] Fig. 11 shows Western Blotting plot of the MCF-7 cell treated with NBM- HD-1.

[0018] Fig. 12 shows the photographs of the neurite outgrowth of the cortical neurons treated with NBM-HD-1.

[0019] Fig. 13 shows the microscopic photographs of the human breast cancer MCF-7 cells treated with different concentrations of NBM-HD-2.

[0020] Fig. 14 shows the mricroscopic photographs of the human breast cancer MCF-7 cells treated with different concentrations of NBM-HD-3.

Detailed Description of the Invention l[- [0021] The present invention relates to novel compounds derived from propolins, Swhich are useful as agents for enhancing the neurite outgrowth and preventing and treating of diseases associated with HDAC, in particular, tumor or cell proliferative diseases. The compounds of the invention are potent in inhibiting growth in cancer cells 0 via differentiation pathway. In particular, they can be used as agents for anti- IO neurodegenerative diseases and human spinal muscular atrophy (SMA).

Compounds of the Invention [0022] Accordingly, the present invention relates to compounds represented by the following formula R2 RS RI R4 R7

(I)

wherein R, and R 2 are each independently OH, OC(=O)alkyl, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkcnyl, N-alkenyl, O-alkynyl, S-alkynyl, N-alkynyl, O-C 3 -scycloalkyl, S-C 3 cycloalkyl, N-C 3 -scycloalkyl, O-unsaturated 5- to 10-membered monocyclic or bicyclic ring, S- unsaturated 5- to 10-membered monocyclic or bicyclic ring, N- unsaturated 5- to monocyclic or bicyclic ring, alkyl, alkylenyl, alkynyl, C 3 8 cycloalkyl, an unsaturated 5- to 10-membered monocyclic or bicyclic ring or a saturated or unsaturated to 10-membered heterocyclic ring comprising at least one ring heteroatom selected from the group consisting of: N, O and S; or R, and R 2 can together form dioxolane;

R

3 and R 4 are each independently OH, OC(=O)alkyl, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, O-alkynyl, S-alkynyl, N-alkynyl, O-C 3 8 cycloalkyl, S-C 3 8 cycloalkyl, N-C 3 8 cycloalkyl, O-unsaturated 5- to O10-membered monocyclic or bicyclic NO ring, S- unsaturated 5- to 10 O-membefe8'monocyclic or bicyclic ring, N- unsaturated 5- to monocyclic or bicyclic ring, alkyl, alkylenyl, alkynyl, C 3 8 cycloalkyl, an 0 unsaturated 5- to O10-membered monocyclic or bicyclic ring or a saturated or unsaturated 5- to 10-membered heterocyclic ring comprising at least one ring heteroatom selected from the group consisting of: N, O and S;

R

5 is C 4 -16 alkyl or C4- 1 6 alkenyl wherein the alkyl or alkenyl is unsubstituted or substituted with one or more C -6 alkyl, OH, halogen, CN, NO, N 3

NH

2 CHO, OR 9

SR,

NR

9 or COOR 9

R

6 is C 2 -1 2 alkyl or C 2 1 2 alkenyl wherein the alkyl or alkenyl is unsubstituted or substituted with one or more C 1 6 a l",MH, halogen, CN, NO, N 3

NH

2 CHO, OR 9

SR

9 or NR 9 or one of R 5 and Ro is hydrogen, halogen or OH, while the other is C 4 16 alkyl or C4- 6 alkylene unsubstituted or substituted with one or more C 1 6 alkyl, OH, NH 2 halogen, CN, NO or N 3

R

7 and R 8 are each independently hydrogen, halogen, OH, NH,, COOH, CHO, CN, NO,

C

1 6 alkyl unsubstituted or substituted with OH, NH 2 COOH, halogen, CN, NO or CHO, -7- O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, O-alkynyl, S-alkynyl or N-alkynyl, or R 7 and Rg may together form a double bond, a C3.

6 cycloalkyl, or a 5- to L membered heterocyclic ring comprising at least a heteroatom selected from the group consisting of N, O and S;

R

9 is phenyl, C(=0)ORo or benzyl; and 00 SR'° is OH, NHOH, NH 2

C.

6 alkyI, phenyl or benzyl; t"- ^0

CH

3

CH

3 SH3CH provided that when RI, R2, R 3 and R4 are OH, R 5 is not F t-13 ii and R 6 is not or H; or when R R 2

R

3 and R 4 are OH and R 5 is H, R 6 is 113 [H3 H3 CH 3 not Hor 3 and pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof.

[0023] In the context of the present specification, the term "alkyl" means straight or branched hydrocarbon chains. The alkyl is preferably Ci.io alkyl. Preferably, the carbon number of alkyl is selected from the group consisting of 1 to 8; more preferably, it is CI.

6 alkyl or CI.

4 alkyl. Examples of alkyl groups include methyl (-CH 3 ethyl

CH

2

CH

3 propyl (-CH 2

CH

2

CH

3 isopropyl (CH 3 2 CH and butyl (-C 4

H

9 [0024] In the context of the present specification, the term "alkenyl" means both straight and branched chain unsaturated hydrocarbon groups, wherein the unsaturation is present only as double bonds. According to the invention, the alkenyl includes one or more double bonds. The alkenyl is preferably C2- 16 alkenyl. More preferably, the carbon 2 number of alkenyl is selected from the group consisting of 2 to 12. Examples of alkenyl groups include ethenyl (-CH=CH 2 propenyl (-CH=CHCH 3 or -CH 2 CH=CH2), butenyl Cl 2

CH=CHCH

3 or -CH=CHCH 2

CH

3 or -CH 2

CH

2

CH=CH

2

-CH

2

CH=C(CH

3

)CH

3

SCH

2 -CH=CH-Cl- 2 -Cl- 2

-CH=CH-CH

3 and -CI-1 2

-CH=C(CI

3

)-CH

2

-CH

2

-CH=C(CH

3 00 I CH 3 [0025] In the context of the present specification, the term "alkynyl" means both

O

straight and branched chain unsaturated hydrocarbon groups, wherein the unsaturation is So present only as triple bonds. Preferably, the carbon number of alkynyl is selected from the group consisting of 2 to 8. More preferably, alkynyl is C2-6 alkynyl or C2-4 alkynal.

Examples of alkynyl groups include propynyl -CH 2 C CH).

[0026] In the context of the present specification, the term "cycloalkyl" means an aliphatic ring (saturated carbocyclic ring). Preferably, the carbon number of cycloalkyl is selected from the group consisting of 3 to 8. Examples of cycloalkyl groups include cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl.

[0027] In the context of the present specification, the term "unsaturated 5- to membered monocyclic or bicyclic ring" means unsaturated 5- to monocyclic or bicyclic (fused or otherwise) ring system, examples of which include phenyl and naphthyl.

[0028] In the context of"the present specification, the term "saturated or unsaturated 5- to 10-membered heterocyclic ring comprising at least one ring heteroatom selected from the group consisting of: N, O and S" means a saturated or unsaturated 5- to heterocyclic ring system comprising at least one ring heteroatom selected L from nitrogen, oxygen and sulphur, each group being optionally substituted by at least one substituent selected from nitro, hydroxyl, oxo, halogen, carboxyl, Ci-C 6 alkyl, C|-

C

6 alkoxy, Ci-C 6 alkylthio, Ci-C'alkylcarbonyl, CI-C 6 alkoxycarbonyl and phenyl.

Examples of the hetercyclic ring includes pyridinyl, pyrazinyl, pyrimidinyl, pyrroyl, 00 ND pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isoxazolyl, thiadiazolyl, oxadiazolyl, thienyl, furanyl, quinolinyl, isoquinolinyl, and the like.

S[0029] In the context of the present specification, the term "halogen" means fluorine, chlorine, bromine and iodine.

[0030] In the context of the present specification, the term "pharmaceutically acceptable salt" includes those formed with both organic and inorganic acids and bases.

Pharmaceutically acceptable acidladdition salts include those formed from mineral acids such as: hydrochloric, hydrobromic, sulphuric, and phosphoric, acid; and organic acids such as: citric, tartaric, lactic, pyruvic, acetic, trifluoroacetic, succinic, oxalic, formic, fumaric, maleic, oxaloacetic, methanesulphonic, ethanesulphonic, p-toluenesulphonic, benzenesulphonic and isethionic acids. Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases, including salts of primary, secondary and tertiary amines.

[0031] In the context of the present specification, the term "prodrug" means a compound which is converted Within the body, by hydrolysis in the blood, into its L active form that has medical effects.

[0032] In the context of the present specification, the term "solvate" means a complex comprising the compound of the invention and a solvent in which they are reacted or from which they are precipitated or crystallized.

00 IN [0033] In the context of the present specification, the term "stereoisomers" are isomeric molecules whose atomic connectivity is the same but whose atomic arrangement in space is different.

[0034] In the context of the present specification, the term "enantiomers" are stereoisomers that are nonsuperiihtosable complete mirror images of each other, much as one's left and right hands are "the same" but opposite.

[0035] According to one embodiment of the compounds of formula of the invention, preferably, RI and R 2 are each independently OH, OCl_ 6 alkyl, OC(=O)Ci.

6 alkyl, O-phenyl or O-benzyl or R, and R 2 together form dioxalene. More preferably, R, and R 2 are each independently OH, OCH 3

OCH

2

CH

3

OCH

2

CH

2

CH

3

OC(=O)CH

3

O-

phenyl or O-benzyl.

[0036] According to one embodiment of the compounds of formula of the invention, preferably, R 3 and R 4 'are.each independently OHI-, OC 1 -6alkyl, OC(=O)Ci.

6 alkyl, O-phenyl or O-benzyl. Mbl'e preferably, R 3 and R 4 are each independently OH,

OCH

3

OCH

2

CH

3

OCH

2

CH

2

CH

3

,.OC(=O)CH

3 O-phenyl or O-benzyl.

-11- C[0037] According to one 'ebodaiment of the compounds of formnula of the

JH

3

H,

H

3 C invention, preferably, R 5 is HOj

H

3

C

3

H

pheiyl phenyl 0o 0 HOJ

O'

BrH 3 H 3

H

3

C

Cj- 4 alkyl Cj- 4 alkyl 0 0 0

"N

HO

HON 3 0

N

E[003 8] According to one embodiment of the compounds of formula of the invention, preferably,

R

6 is

H

3 CjH

HOJ

H

3

C

N

3

X

H

3

C

-1 .2-

H

3 Brj

H

3

C

Phenyl 0 0 OH

H

PhS cH- 3 Ci- 4 alkylO 0H( C 4 aflyl 0o> 0 phenyl 0 0JO 3 or (0039] According to the inv ntidni, the preferred compound of formula of the invention is selected fromn the group consisting of:

H

3

CX

.OBn

H

3

G

-14--

H

2 NOe MeOe

HC

CIA Oo 0

H

3 113 M CH3OMe H3HH 3M 00 Br IND H 3

M

H

3 C Me1G O~ 0

CH

3 OMe 0.

H

3 1-13 Me

H

3 C Me" 0 Phs>

CH

3 OMe 0 -16- 2007216781 14 Sep 2007

CH

3

CH

3

OCH

3 H3C

OCH

3

H

3 CO 0

HICY

CH

3 OH 0 00

CH

3

CH

3

OCH

3 HO>J

OCH

3 ci

H

3

C

H

3 CO, 0

H

3

C

HO

K

CH

3 OH 0

CH

3

CH

3

OCH

3

OCH

3

H

3

C"

H

3

C~

HO

CH

3 OH 0 and -18-

CH

3

CH

3 HO.K 1

H

3

C

CH

3 O 1:2 CH3 OH 0

,OCH

3 00 0O

IN

[0040] The present invention also relates to a stereoisomer according to the compound of formula and is represented by the following formula (II):

R

4

R

7 wherein R 1 R2, R3, R4, Rs, R6, R7 and Rg are defined as those in formula [0041] According to a more preferred embodiment of the invention, the compound of formula is that having the following formula (IlI), or (VI):

H

3 CO 0

H

3

C

HO

CH

3

OCH

3 0 (111)

,OCH

3

H

3

C.

(IV)

CH

3

CH

3

OCH

3

H

3 CHC0

CH

3 0OH 0

,OCH

3 HO^

I

CH

3 OH 0 00 IN (VI) [0042] According to the invention, the compounds of formula of the invention Scan inhibit HDAC and thus can be used as agents for prevention or treatment of diseases associated with histone deacetylase (HDAC). In addition, the compounds of the invention significantly inhibit growth of multiple cancer cell lines, including those of rat C6 glioma, human glioblastoma, human breast cancer cells, human leukemia cells, and human melanoma cells. The mechanism for inhibiting the growth of cancer cells may be via differentiation pathway, in particular via induced differentiation and regulated cell cycle regulator gene expression, including those of p21 and cyclin B1. In addition, the compounds of formula of the invention can mediate neuronal differentiation of neural stem cells and thus can be used as agents against anti-neurodegenerative diseases.

[0043] For the therapeutic uses of the compounds of the invention, the dosage administered will, of course, vary with the compound employed, the mode of administration, the treatment desired and the disorder indicated. The daily dosage of the compound of formula may be in the range from 1 mg/kg to 40 mg/kg. The invention provides the methods of inhibiting HDAC, treating tumor or cell proliferative disease, neurodegenerative diseases and human spinal muscular atrophy and enhancing the neurite Soutgrowth in a subject, comprising administrating to the subject a therapeutically

(N

e2 effective amount of the compounds of the invention, respectively.

General Synthesis of the Compounds of Formula I of the Invention [0044] The compounds of the present invention can be prepared by any conventional means. Suitable processes for synthesizing these compounds are provided t"- ID in the examples. Generally, compounds of formula I can be prepared according to one of the described synthetic schemes below: t(, Scheme 1 CH.1 OH 3 O H HO 3.K

K

2 0 3

DMS

H

3 C ~acetone,

OH

3 OH 0

OH

3

OH

3 OMe

H-

3 ON I OMe MeO 0

H

3 0 C

OH

3 OMe 0

OH

3

OH

3

OH

OH

H

3 C

I

HO 0

AC

2 O, pyr HC r

CH

3 OH 0

OH

3

OH

3 OAc

H

3 C NOAc 11AcO 0

OH

3 OAc 0 3 OH,, OH 3

OH

O0H HO 0 'N K 2 00 3 5, Mel

H

3 O acetone, rt

OH

3 OH 0

OH

3

OH

3 OMe

H

3 ON NOMe 11CMeO N 0

H

3 0

OH

3 OH 0 Scheme 2

CH

3

C;H

3

OH

t HC) 0 1 O iL ncetoiie Gi' 3 ODH 0 C.N OB11

H

3 ElnO l Q

GH

3 Gil 3

OR,

R IO o H 2 S 4 T F H, C I

I-

CHI~ ORI HO Gil, Cil, OR, HICL NZ OR, H3C Cil OR, 6a R,7R,=H 6b Rj=F1 2 =Klo 6c R,.-R,=Ac 6d R, =Moi., R~H 6e R,=R 2 -Bn1 I R:=R,.H 2 R,=R 2 =.lu 3 R,-R 2 ?-Ac 4 R,=Me.R 2

=H

6 R.=R2=Bil Plil(OAc)~., KeCN NC OR, il,

H

2 S0- 4

-THF

PH

3 R0 HO Cil 3

CH,

3

OR,

H,G R R,O N 0 N H C,

CH

3 OR, 0) 7a R,-R2=H 7b Rj=R 2 =Me 7c RI=R2zAc 7d R,=Me, R2=i 7e R,=R2=l) 1 2~ R,=R2=Me 3 R,--Ac 4 R,=Me. R2=H RI=R2=Btn -24- Scheme 3 0 0 ci

C)

Co Cl i CH-, OR 1 CH, CHi OR,

H

3 C 113C NaBH,. WO S 3 C(310

(,H

3 k-)R 2 0 Ct OR, OH I R,zR2H 8a RtR 2 rH 2 RI=R 2 =M"e 8b RIzR 2 zMe 3 R 1

=R

2 2=Ac Sc R 1

=R

2 =Ac 4 R.=Mrt R =H 8d R.=Me~ R-.=H

I):R

2 =Bi1 Se R.=R 2 =Fln NH NH OR OR HO C 3 O R I N 0> NO 2 0.HF H 1 C: OR, HO~f

OH

3

C"

3 oR 2 Sa P 1

=R

2

=H

Sb R,=R 2 =Me i0a R,=R 2 zH Sc RzR,zAc l0b R,=R 2 =Me Sd R,41e.' R2=l l0c RmR 2 =Ac Se R,=R 2 =811) 10d R,14e. R 2

=H

i~e R,=R-,=Bn McP!A

CH

3

CI

2 C13 C,

R

CH-, CH 3

OR

1 .H ei R N OR, HCY N OR P.O 0.0'N

R

1 1> RNH 2

CH'

2

CI,

N- 2) H2,5C-THF. 1 11 7 o Ila R,=R 2 =H 12a R,=R 2

=H

lib R'=R 2 =1e 12b R,=R2=Mu lit RI=R 2 zAc 12c R,=R,'Ac lid R,'Me. n 2 =ii 124 R 1 'Mie. P~rH lie RzR:rBii 12e PR,4,B Scheme 4 HO Cll CHI OhMe

'NC

H'C

DE

N

3 CH, CH, CINEP AD, Ph 3 P H~ NO~e HN,

H

3

C

N

3

H

3 eC 13 NoBH4. MeOH 0' 3 OMCe H,N'NC e

H

3

C

H,N CH,. olke 0 HCI CH OH 3 oM'i C, Ome

H

3

HO;:

CHI MIA 0 P~r 3

H)

(:l1 I HH Ofhie O I I' CH, 01,) 0 l0b SOCIZ. pyr CH2CI 2 PhSH, H2I-1 2 0 4

H

3

C

H I O eC 16 C.H 3

CH

3 ONIe PhS)Kt WNOe

'NC

HCH

3 orle 0 17 Scheme Ho OMe MeO N0 RCOCI, pyr rt H OD OMeO0 RO j RINaHOe RN OMe RO;! WOMeO0 19a: R=CH 3 19b: R=CH 3

CH

2 19c: RBn 19d: R=(CH 3 2

CH

R y 1 omOM 0MeO N0 0 1 0) OMe 0 18a: R=0H 3 18b: R=CH 3

CH

2 18c: R=Bn 18d: R(CH 3 2

CH

-7- Scheme 6 BrCH 2 00 2 Me NaH, DMF NaOH, MeOHwia-,

NH

2 OH, EtOH, KOH Scheme 7 HC(OMe) 3 TsOH MeOH, rt HO 0

KC

3

RI

HO 'N Acetone, 0 or AC 2 O. pyr, rt OH 0 23 R=Me, i-propyI, Bn, Bz HO 0\ /0 H'N

H

2 SO,-THF RO 0 'N BrCH 2

CO

2 Me I HO INaH,

DMF

OR 0 24a R=Me 24b R=i-propyI 24c R=Bn 24d R=Bz 24e R=Ac R=Me 25b R=i-propyI 25c R=Bn 25d R=Bz 25a R=Ac NaOH. MeOH(aq) 26a R=Me 26b Ri-propyl 26c R=Bn 26d R=Bz 26e R=Ac

NH

2 OH, EtOH. KOH 27a R=Me 27b Ri-propyl 27c R=Bn 27d R=Bz 27e R=Ac 28a R=Me 28b R=i-propyl 28c R'=Bn 28d R=Bz 28e R=Ac Scheme 8 OH OMe C~1OH OMe HO 0 "1 0. M MeO N 7acetone 7~ OH 0 Oe 29 e OMe 00MeO 0 00

H

2 S0 4

-THF

O H OH0 OMeO0 31 OH

OH

HOC 0 N OH 4HSOTHF HO 0 OH OHO 0 OHO0

OH

32 3 OH OAc HO N OH Ac O, pyr AcO 0 N~ 7 rt. overnight OHO 0 OAcO 0 32 34 OH OMe HO 0 CH K,00 3 DMS MeO N0 N~ OH ip overnight e0P 32 [0045] Methylation of RI, R 3 and R 4 of the compounds of formula may be (lone by the following procedure: mixing the reactant, K 2 C0 3 and acetone with Me 2

SO

4 (DMS), and heating and stirring the solution; refluxing the resulting solution under a t controlled atmosphere (for example, under N 2 for a period; after removing the organic

O

Csolvent, dissolving the residue in a certain organic solution and washing it with water; rdrying the organic layer by evaporation under reduced pressure and purifying the product by a silica gel column (EtOAc: n-Hexane 1:4).

[0046] The addition of OH.to a double bond in R 5 or R 6 may be done by the 00 r following procedure: adding the solution of reactant in THF to H 2 S0 4 in an ice bath; after the addition, stirring the solution for a period and then diluting it with water; extracting O the mixture with a suitable organic solution, for example, CH 2 Cl 2 drying the combined organic layers by evaporation under reduced pressure to give a residue, and purifying the io residue by a silica gel column MeOH/CH 2

CI

2 [0047] The preparation of compounds 6a-e and 7a-e were shown as scheme 1 and 2 described. The Propolin G, that is a propolin derivative known in the art, was methylated, acetylated, rigio-sclectively methylated and benzylated with the corresponding reaction conditions to yield compounds 2-5 and followed by acidic hydration of compounds 1-5 provided target dihydroflavones 6a-e, respectively.

lodosobenzene diacetate oxidation followed by acidic hydration of compounds afforded the corresponding flavones 7ae.

[0048] Compounds 10a-e and 12a-e were prepared as outlined in scheme 3.

Sodium borohydride reduction followed by dehydration of compounds 1-5 gave the corresponding compounds 9a-e. Acidic hydration of 9a-e yielded target compounds e, respectively. MCPBA epoxidation of compounds 9a-e provided the epoxides lla-c, -31t and the ensuing nucleophilic reaction with corresponding amines such as methylamine,

O

C' ethylamine and enzylamine afforded target compounds 12a-e, respectively.

r[0049] Compounds 14-17 were synthesized as scheme 4 described. Mitsunobu reaction of lOb gave desired azide 13 and the ensuing sodium reaction converted azide to tertiary amine 14. Bromination with phorsphorus tribromide, thiolation with phenol thiol 00 (PhSH) and chloination with thionyl chloride of compound 10b provided bromide

ID

Sthiol ether 17 and chloride 16, respectively.

[0050] Compounds 18a-d and 19a-d were prepared as scheme 5 described.

Acylation of compound 10b with corresponding acyl chlorides such as acetyl, propionyl, benzoyl and isobutyryl gave compounds 18a-d, respectively. Alkylation of compound with corresponding alkyl iodidessuch as methyl, ethyl, benzyl and isopropyl iodide afforded compounds 19a-d, respectively.

[0051] Compound 22 was synthesized as scheme 6 showed. Reaction of tertiary alcohol of compound 6b with ethyl bromoacetate under sodium hydride condition gave compound 20. Basic hydrolysis of compound 20 afforded compound 21 and following reaction with hydroxylamine yielded hydroxamic acid 22.

[0052] Hydroxyamic acids 28a-e were prepared as scheme 7 described. Reaction of compound 1 with trimethyl orthoformate under acidic conditions gave acetonide 23 and the ensuing alkylation with alky iodides such as methyl, isopropyl and benzyl, or acylation with benzoyl chloride 'and acetic anhydride afforded compounds 24a-e, respectively. Acidic hydration of two terminal olefins of compounds 24a-e provided compounds 25a-e and the ensuing reaction with ethyl bromoacetate gave compounds Q 26a-e. Basic hydrolysis of compounds 26a-e afforded compounds 27a-e and the ensuing c, reaction with hydroxylamine yielded hydroxamic acids 28a-c, respectively.

Pharmaceutical Composition of the invention [00533 The compounds of formula and pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof may be used on their own but 00 will generally be administered in the form of a pharmaceutical composition in which the

NO

Ci fonnula compound/salt/solvate (active ingredient) is in association with a pharmaceutically acceptable adjuvant, diluent or carrier. Depending on the mode of administration, the pharmaceutical composition will preferably comprise from 10 to wt% (percent by weight), more preferably from 30 to 50 wt%, still more preferably from to 70 wt%, and even more preferably from 70 to 100 wt%, of the active ingredient, all percentages by weight being based o6ntotal composition. In addition, the pharmaceutical composition of the invention may further comprise other agents for prevention or treatment of diseases associated with histone deacetylase (HDAC).

[0054] The pharmaceutical compositions may be administered systemically, e.g., by oral administration in the form of tablets, capsules, syrups, powders or granules; or by parenteral administration in the form of solutions or suspensions; or by subcutaneous administration; or by rectal administration in the form of suppositories; or transdermally.

[0055] The compounds and pharmaceutical compositions of the invention are an HDAC inhibitor and can be retained long in the cells and continuously induce the acetylation of histone H4. They ar H

I

DAC inhibitors inducing differentiation of cells and neural stem cells. In addition, the compounds of the invention significantly inhibits L I HDAC activity. The compounds of the invention significantly decrease both S and G2/M

O

phases of the cells in a dose-dependent manner and change the morphology of cancer cells. Therefore, the compounds of the invention can treat tumor or cell proliferative disease. Moreover, the compounds of the invention can enhance the neurite outgrowth and treat neurodegenerative diseases and human spinal muscular atrophy (SMA).

00 r- EXAMPLE The following examples illustrate preferred methods for synthesizing and using the Scompounds: Example 1 Preparation of 3',4',5,7-Tetramethyl-propolin G (2) [0056] To the mixture of Propolin G 5 g, 10.16 mmol), K 2 C0 3 (16.27 g, 117.89 mmol) and acetone (280 mL) Me 2

SO

4 (15.76 mL, 126 mmol) was added and the resulting solution was heated to reflux under nitrogen for 24 h. After removal of the organic solvent, the residue was dissolved in CI-1 2 C1 2 (80 mL) and washed with H20 (40 mL x 3).

The CH-,C layers were dried overNaRS0 4 and evaporated under reduced pressure. The residue obtained was purified by a silica gel column (EtOAc: n-Hexane 1:6) to give compound 2 (4.00 g, 'H-NMR (400 MHz, CDCI 3 O 7.26 (1H, d, J=8.6 Hz), 6.86 (1H, d, J=8.6 Hz), 6.27 (1H, 5.50 (1H, dd, J=2.5, 13.5 Hz), 5.14-5.11 (1H, 5.11- 5.10 (1H, 5.02-4.99 (IH, 3.87 (3H, 3.83 (311, 3.79 (6H, 3.50 (1H, dd, J=6.6, 15.2 Hz), 3.43 (111, dd, J=5.8, 15.2 Hz), 3.00 (1H, dd, J=13.5, 16.7 Hz) 2.68 (111, dd, J=2.6, 16.7 Hz), 2.00-1.92 (2H, 1.75 (3H, 1.70 (3H, 1.65 (3H, s), 1.60 (3H, 1.52 (311, 3 C-NMR (100 MHz, CDC13) O 189.4 164.0 163.3 159.6 153.0 147.2 135.6 134.2 131.5 131.3 129.8 124.1 122.8 122.7 122.1 118.2 110.3 108.7 95.6 75.9 61.8 ri 60.7 55.7 55.7 45.1 39.6 26.6 25.7 25.6 24.9 22.0 17.7 17.6 16.3 1-REIMS Calcd for C 34

H

44 0 6 548.3142, Found 548.3140.

00 r-Example 2 Preparation of 6-(2-Hydroxy-2-methylbutyl)-2'-(7-hydroxy-3,7dimethyloct-2-enyl)-3',4',5,7-tetram et hoxyflavanone (6b) [0057] To a solution of compound 2 (7 g, 12.77 mmol) in TI-IF (170 ml-) 49%

H

2 S0 4 (140 mL) was added in an ice bath. After complete addition, the reaction mixture was stirred at rt for 8 h and then diluted with H 2 0. The reaction mixture was extracted with CH 2

CI

2 (100 rnL x The combined organic layers were dried over Na 2

SO

4 and evaporated under reduced pressure to give a residue, which was purified by a silica gel column (n-hexanc:EtOAc~l:lVl:3) to give pure oil 6b (2.30 g, 'lI--NMR (400 Mliz, CDCI 3 D 7.27 (111, d, J=8.6 Hz), 6.87 (1H, d, J=8.6 6.28 (1H1, d, J=2.6 Hiz), 5.49 (11-1, dd, J=2.4, 13.6 Hz), 5.05 (111i, t, J=6.0 Iz), 3.87 (3H1, 3.84 (311, s), 3.79 (6H1, 3.52 (11-1, dd, J=5i5,. 15.2 Hz), 3.43 (I149 dd, J=7.69 15.2 Hz), 2.97 (111, dd, J=8.09 16.7 Hz), 2.70-2.59 (31H, in), 1.93 92H, t, J=6.4 Hz), 1.65 (3H9 1.64-1.60 (21-1, in), 1.41-1.32 (411, in), 1.25 (611, 1.15 (31-1, 1.14 (311, 1 3 C-NMR (100 M~lz, CDCI 3 189.4 164.0 163.3 159.6 153.0 147.2 135.6 134.2 131.5 131.3 129.8 124.1 122.8 122.7 122.1 118.2 110.3 108.7 95.6 75.9 61.8 60.7 55.7 55.7 45.1 39.6 26.6 25.7 25.6 24.9 22.0 17.7 17.6 16.3 l-IREIMS Calcd for C 3 4 1- 48 0 8 584.3338, Found 584.3344.

Example 3 Preparation of 6-Gera nyl-3',4',5,7-tetrametho xyflava none 0058] To the mixture 29 lPropolin C, 128 mg, 0.31 mmol), K 2 C0 3 (431 mg, 3.1 00 r- rmmol) and acetone (15 mL) was added Me 2

SO

4 (0.25 mL, 2.48 rnmnol) and the resulting c-I solution was heated to reflux under nitrogen for 24 h. After removal of the organic solvent, the residue was dissolved in CI-1 2 C1 2 (50 mL) and washed with H 2 0O (50 mL x 3).

The organic layer was dried over NaSO 4 and evaporated under reduced pressure. The i0 residue obtained was purified by at silica gel column (EtOAc: n-Hexane=l to give compound 33 (107 mg, 'H-NMR (400 MHz, CDCI 3 6.99-6.97 (211, in), 6.88 (111, d, J=8.8 Hz), 6.31 (111, 5.33 (111, dd, J=2.8, 13.3 Hz), 5.11 (111, td, J=1, 6.9 liz), 5.04(11-1, td, J=1.3, 5.5 Hz), 3.90 (311, s, OMe), 3.88 (31-1, s, OMe), 3.81 (311, s, ON~c), 3.80 (3KH, s, OMe), 3.34 (1KH, dd, J=7.2, 14.1 Hz), 3.26 (111, dd, J=7.2, 14.1 Hz), 3.02 (111, dd, J=13.3, 16.7 liz), 2.74 (11-1, dd, J=2.8, 16.7 I1z), 2.04-2.00 (211, in), 1.96- 1.92 (2H, mn), 1.74 (31-1, 1.62 (3H, 1.55 (3H, 1 3 C-NMR (100 MI-z, CDCI 3 189.1 164.1 163.0 159.4 149.5 149.4 131.3, 131.2 124.4 122.9 118.9 109.5 1 8R, 9 95.7 79.2 61.9 56.1 56.0 55.9 45.6 39.8 26.7 25.7 22.0 17.7 16.1 IIREIMS Calcd for C 29

)H

36 0 6 480.25 10, Found 480.2511.

Example 4 Preparation of 6-(2,6-Dihydroxy-2,6-dimetlhyl-octyl)-3',4',5,7ri tetramethoxyflara none (31) [0059] To a solution of compound 30 (80 mg, 0.17 rnrnol) in THE (6 mL) 49% F1 2 S0 4 (4 rnL) was added in an ice bath. After complete addition, the reaction mixture was stirred at rt for 8 h and then diluted with 1F120. The reaction mixture was extracted 00 with CH 2 CI (50 nil x The combined organic layers were dried over Na')SO 4 and c-i evaporated under reduced pressure to give a residue, which was purified by a silica gel column MeOH/CH 2 Cl 2 to give pure oil 31 (44 mg, 'H-NMR (400 MHz,

CDCI

3 016.99-6.97 (211, in), 6.88 (111, d, J=8.8 Iz), 6.31 (11i, 5.33 (11H, dd, J=2.8, 13.3 Hz), 3.90 (311, s, OMe), 3.88 (314, s, ON'e), 3.84 (311, s, OMe), 3.82 s, OMe), 3.02 (111, dd, J=13.3, 16 lHz), 2*.15(11II, dd, J=2.8, 16Hiz), 2.62-2.58 (21-1, in), 1.63-1.59 (711, in), 1.49-1.48 (2H, mn), 1.23 (31H, 1.22 (31-1, 1.21 (311, 3 C-NMR (100 MIHz, CDCI 3 189.1 164.1 163.0 159.4 149.5 149.4 131.2 119.0 118.9 111.3 109.5 108.9 95.8 72.9 7 1.1 62.1 56.0 55.9 45.5 44.5 42.3 42.1 41.5 29.4 29.3 26.9 26.8 18.8 17.5 IIREIMIS Calcd for (M-18) 498.2602, Found 498.2610.

Example 5 Preparation of Porpolin A (33) [0060] To a solution of Propolin D (compound 32, 100 mg, 0.24 mmol) in THF (6 mL-) 49% H 2 S0 4 (4 ml) was addt~in an ice bath. After complete addition, the reaction mixture was stirred at rt for 8 h and then diluted with 1W2. The reaction mixture was extracted with CI- 2 C1 2 (50 ml- x The combined organic layers were dried over 3 7 Na 2

SO

4 and evaporated under reduced pressure to give a residue, which was purified by a silica gel column MeOH/CI-C 2 to give pure oil 33 (42 mg, 'H-NMR cJ (400 MIHz, NMeGI) 6.87 (11-1, d,.J=8.4 Hiz), 6.71 (111, d, J=8.4 Hiz), 5.88 (211, dd, 3=1.9, 3.3 Hz), 5.47 (1 H, dd, J=2'6 13 Hz), 5.12 (1KH, dd, J=5.79 6.7 Hz), 3.47 (21-1, d, J=6.6 liz), 3.10 (1li, dd, 3=13.4, 17 Hiz) ,2.60 dd, J=2.79 17.1 Hiz), 1.94 (111, dd, 00 r- 13.4 l-Iz), 1.64 (11-1, d, 3=0.5 li-z), 1.43-1.41 (2H4, in), 1.37-1.34 (21-1, in), 1.13 (Ki 1.12 s) C-NN'R (100 MI~z, CDC1 3 198.2 168.5 168.4 165.5 165.4 165.2 146.5 144.5 135.8 129.7 128.2 124.7 118.7 113.6 103.2 97.1 96.2 (d),77.8 71.5 44.3 43.7 41.2 29.2 29.1 25.4 23.7 16.2 Example 6 3',4',5,7-Tretraacetyl -Prooun D (34) (0061] To a solution of Proj5~Ain D (compound 32, 124 mg, 0.29rnmol) in pyridine (4 mnL) acetic anhydride (2 ml) was added and the reaction mixture was stirred at room temperature for 6 h. EtOAc (25 mnL) was added to the reaction mixture and the mixture was washed with 0. 1 N HCI (10 mL x3). The organic layer was dried over Na 2

SO

4 and evaporated under reduced pressure to give a residue. The residue was purified by a silica gel column (CH 2

CI

2 to yield pure 34 oil (l39ing, 'l1-NN'R (400 MHz, CDCI 3 7.49 (11-1, d, J=8.6 7.18 (111, d, J=8.6 li-z), 6.72 (11-1, d, 3=2.2 Hz), 6.53 (111, d, 3=2.2 i1z), 5.60 (11-1, dd, 3=2.5, 13.8 5.01 td, J=5.3, 4.6 Hz), 4.94 (111, td, 3=1.0, 5.8 11z), 3.35 (11-1, dd, J=7A '157 l11z), 3.26 (1H, dd, 3=5.3, 15.5 Hz) ,2.98 (1H-, dd, J=13.8, 16.8 Hz), 2.36 2,27 (611, 2.26 31-1), 2.02-1.91 (51-1, nm), 1.64 -38- (31-1, 1.62 (311, 1.55 (31-1, 1 3 C-NMR (100 MNz, CDC 3 188.8 169.2 168.1 168.0 167.9 163.2 155.9 151.3 142.8 140.9 137.0 135.2 133.5 131.6 124.5 123.9 121.6 120.7 111.6 110.7 109.0 76.1 44.8 39.4 26.5 25.6 25.5 21.1 21.0 20.7 20.3 17.7 16..3j(q) 00 Example 7 3',4',5,7-Tetramethyl propolin D [0062J To the mixture 32 (128 mg, 0.31 mnmol), K 2 C0 3 (431 mng, 3.1 mmol) and acetone (1 5 rnL) N'e 2

SO

4 (0.25 mL, 2.48 mmol) was added and the resulting solution was o0 heated to reflux under nitrogen for 24 h. After removal of the organic solvent, the residue was dissolved in Cl-1,C1 2 (50 mL) and washed with H]20 (50 mL x The organic layer was dried over Na 2

SO

4 and evaporated under reduced pressure. The residue obtained Was purified by a silica gel column (EtOAc: n-Hexane=1:4) to give compound 35 (107 mg, '11-NMR (400 M"z, QDCI,3) 7.26 (111, d, J=8.6 Hz), 6.85 (IH, d, J=8.6 Hz), 6. 10 (11H, d, J=2.3 14z), 6.07 (1 H, d, J=2.3 Hz), 5.34 (11-1, dd, J=2.6, 13.5 5.04 (11H, td, J=5.3, 4.6 Hz), 4.99 (111., td, J=1.0, 5.8 3.88 (31-1, 3.86 s) 3.80 (3H, 3.74 (3H1, 3.46 (11H, dd, J=6.6, 15.2 Hiz), 3.44 (1 H, dd, J=5-8, 15.2 Hiz), 3.02 (11-1, dd, J=13.5, 16.5 Hz), 2.69 (111, dd, J=2.6, 16.5 Hz), 2.00-1.97 (2H, in), 1.94-1.92 (211, in), 1.65 (3H, 1.60 (31H, 1.52 (311, s).

Example 8 3',4',7-O-Triniethylpropolin G (4) -39-

.OCH

3 1- 3

C

00

IDCH

3 OH 0 C[00 63] To the mixture of Propolin G (2.30 g, 2.75 mmol), K 2 C0 3 (1.94 g, 13.99 mrnol), and acetone (80 ml) Me 2

SO

4 (2.17 mL, 17.35 mmol) was added and the resulting solution was stirred at r1 for 0.5 h, and then heated to reflux under nitrogen for 6 h. After removal of the organic solvent, the residue was dissolved in CH 2

CI

2 (40 ml-) and washed with H 2 0 (40 mL x 3).

The CH-C1 2 layers were dried over Na 2

SO

4 and evaporated under reduced pressure. The residue obtained was purified by a silica gel column (EtOAc-n-Hiexane=l:8) to give 4 (954 m~g, 1 H-NM R (400 M Hz, CI)C1 3 [l112.06 (111, 7.26 (1 H, d, J=8.4 liz), 6.86 (1 H, d, J=8.4 Hz), 6.03 (1IH, 5.50 (11-1, dd, J=2.4, 13.4 1lIz), 5.17-5.14 (1IH, in), 5.04-5.00 (21-1, in), 3.87 (31-1, s), 3.79 (3H, 3.79 (6H, 3.46-3.45 (21-1, in), 3.25-3.23 (211, 3.05 (111, dd, J=13.5, 16.7 11z) 92.70 (111, dd, J=2.69 16.7 Hz), 2.02-1.94 (711, in), 1.75 (311, 1.67 (311, 1.66 1.61 (311, 1.58 (3H, s) Example 9 6-(2-Hydroxy-2-methylIbutyl)-2 '-(7-hydroxy,-3,7-dimthyloct-2-Cfl)-5 hydroxy-3',4',7-trimthox),flavalofe (6d)

OCH

3

H

3

C

HO)

00 CH- 3 OH 0' IND (NBM-HD-3) E 0064J To a solution of compound 4 (530 mng, 0.99 mmol) in THF (15 mL) 49% H 2 S0 4 ml) was added at ice bath. After complete addition, the reaction mixture was stirred at room temperature for 8 h and then diluted with H 2 0. The reaction mixture was extracted with CH 2

CI

2 (30 mL x The combined organic layers were dried over Na 2

'SO

4 and evaporated under reduced pressure to give a residue, which was purified by a silica gel column (nhexane:FtOAc=l:l) to give pure oil 6d (191 mg, '1i-NMIR (400 Mlhz, CDCI 3 0 12.06 (1l1, brs), 7.27 (11H, d, J=8.6 lIN), 6.86 (111, d, J=8.6 liz), 6.05 5.50 (Ili, dd, J=2.6, 13.6 Ilz), 5.03 (Ili, t, J=6.2 l11z), 3.87 (311, 3.80) 3.48 (11-1, dd, J=5.5, 15.2 Ilz), 3.42 (111, dd, 3=6.1, 15.2 liz), 3.06 (11i, dd9;J=13.6, 17.1 liz), 2.70(11-1, dd, J=2.7, 17.1 liz), 2.65- 2.61 ni), 1.95-1.92(211, in), 1.65 (311, 1.41-1.35 (511, in), 1.26 (6H1, 1.15 (311, 1.14 (311, s).

Example 10 Other Compounds [(0065] The Following two compounds are prepared according to the methods as stated above.

CH

3

CF-

H

3

C"

H

3 C

HO

JCH

3

OH

13 OCH 3 1. OCH3 0

CH

3

CH

3

OCH

3

H

3 C O 0

H

3 C, 1

CH

3 OH 0 (NBM-HD-2) 11 Inhibition of Cancer Cell Growth by the Compound of the Invention NBM-1-1D-I)

H

3

CH

3

OH

H

3 C OH CH 3

CH

3 OMe HO.. 0 K 2 C0 3 .DMS H 3

C

SH

3 Ci I acetone, HC MO

SCH

3 OH OT

CH

3 OMeO 2 0 H 3

CH

3 OMe .CHO 3

CH

3 OMe OMe OMe

H

3 C H 3C MeO 0 H 2 SO,-THF OMe 0

H

3 C rt H3C SHO I

CH

3 OMe O CH 3 OMe O NBM-ID-1 [0066] The cancer cell line, rat C6 glioma cells, was cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco) supplemented with penicillin G, streptomycin sulphate, 0.5 mM of L-glutamine and 10% fetal bovine serum (FBS; Gibco) at 37 s CO 2 and 95% relative humidity. For all experiments, the cells were seeded at a density of 3x10 5 per well in 6-well plates. After 24 hours, the cells were treated with different concentrations of the compound NBM--ID-1 the compound of formula Ill depicted herein). After 48 hours, the cells were observed and counted. According to the results shown in Fig. 1, NBM-HD-I can arrest the growth of rat C6 glioma cells. After the C6 glioma cells were incubated with 2.5 ,ug/mL (see Fig. 5 pg/mL (see Fig. 1(A)-c) and 10 pg/mL (see Fig. of NBM-HD-I for 48 hours, the density of the cells reduced dramatically in comparison with that of control (see Fig. The results obtained by cell counting showed the same tendency (see FIG. The above- Q mentioned results indicate that NBM-HD-1 can inhibit the growth of C6 glioma cells in a C dose-dependent manner.

[0067] lxl06 of rat C6 glioma cells were treated with various concentrations of NBM-HD-1 (0 pg/mL, 2.5 pg/mL, 5 ug/mL, and 10 ug/mL) for 72 hours. The treated cells were trysinized and collected. The cells were resuspended in 200 uL PBS and then 00 r fixed by adding 800 pL of cold 100% ethanol. The resulting cells were fixed overnight at -203. The cell pellets were collected by centrifugation, resuspended in 1 mL of Shypotonic buffer Triton X-100 in PBS and 1 ug/mL RNase and incubated at 37L1 for 30 minutes. Then, 1 mL of PI solution (50 pg/mL) was added to the resulting cell pellets. The mixture was allowed to stand at 40 for 30 min. The DNA amounts of the cells were analyzed by FACScan Cytometry (Becton Dickinson) (see Fig. The results of Fig. 2 showed that NBM-HD-1 can significantly inhibit C6 glioma cell growth through modulating the cell cycle arrest on the GO/G I phase in a dose-dependent manner.

Example 12 Inhibition of Cell growth and induction of Differentiation of Cancer Cells by the Compound of the Invention (NBM-l-D-I) [0068] For the rat C6 glioma cells, the cell cycle related to mRNA expression was examined by RT-PCR. The total RNAs were isolated from the treated C6 cells by using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. The cDNAs were produced from 500ng of total'RNAs using ReverTra-Plus-TM (TOYOBO). The RT product (lpl) was amplified by PCR with primers to analyze several genes of cell cycle by using GAPDH as an internal control. The results were shown in Fig. 3. According to the results of Fig. 3, NBM-HD- can modulate the expression of some cell cycle O regulators. The results showed that NBM-HD-1 can decrease the expression of cyclinDI CK and cyclinB 1 in a dose-dependent manner. On the contrary, the expression of p21 was increased.

[0069] After the C6 glioma cells were incubated with 10 ,g/mL NBM-HD-I for 24 hours, they were fixed and analyzed by the traditional immuno-fluorescent staining 00 r method. Staining of glia was carried out by using glial-specific GFAP antibody (SIGMA)

\O

04 as the primary antibody and a fluorescence labeled rabbit immuno-globulin (SIGMA) as the secondary antibody to bind with the primary antibody. The cells which are GFAPpositive were excited by a specific light source to emit fluorescence. Also, the nuclei were stained with DAPI. The staining'results were shown in Fig. According to Fig.

NBM-HD-1 can induce the GFAP expression of C6 glioma cancer cells. In contrast with the control group, more GFAP proteins were detected in the photos of the cells being treated with 10 pg/mL of NBM-HD-1. The middle row showed the photos of the cells with DAPI staining in Fig. 4(A).

[0070] The GFAP mRNA expression was examined by RT-PCR. The results of Fig. 4(B) showed that the expression of GFAP was increased in a dose-dependent manner.

These results indicated that NBM-HD-I can induce the cell differentiation of C6 glioma cancer cells according to the increase of GFAP expression.

Example 13 Increase of the Atcuiulation of Hyperacetylated Histone in the Cancer Cells Treated with the Compound of the Invention (NBM-HD- 1) [0071] Accumulation of hyperacetylated histone H4 was analyzed in the cell C lysates by using Western Blotting and the antibody directed against acetylated histone H4 S(Upstate). The C6 glioma cells were seeded at a density of Ix 10 6 per 10cm culture dish.

After 24 hours, the cells were treated with 10 ug/mL of NBM-HD-1 or 4mM sodium butyrate for several hours. The whole-cell lysates were prepared by using denaturing 00 r SDS sample buffer and then separated on 15% SDS-polyacrylamide gels. As shown in CN Fig. 5, both sodium butyrate and NBM-HD-1 can increase the accumulation of O hyperacetylated histone H4. The amount of acetylated histones was hardly detectable in untreated C6 glioma cells. The amount of acetylated histone H4 of the cells treated with 4mM sodium butyrate for 2 hours increased. Sodium butyrate was then removed from the culture medium. After 6 hours, the amount of acetylated histone H4 decreased. The accumulation of acetylated histone H4 increased in the cells treated with NBM-HD-1 for 2 hours. After removal of NBM-HID-1, the amount of acetylated histone H4 increased over time. The highest histone acetylation appeared at 6 hours after the compound had been removed. These results indicated that, similar to sodium butyrate, NBM-HD- is an HDAC inhibitor. Because NBM--ID-I is more hydrophobic than sodium butyrate, NBM-HD-1 can be retained in the.cells longer than sodium butyrate and continuously induce the acetylation of histone 1-14. Example 14 Inhibition of HDAC Activity by the Compound of the Invention (NBM-HD-1) [0072] The C6 glioma cells were treated with different doses of NBM-HD-1 and sodium butyrate After 24 hours, the cells were harvested to extract the nuclear -46~ O proteins by NucBuster M Protein Extraction Kit (Novagen) as described by the Smanufacturer's instructions. These extracts were then used in HDAC Activity Assay Kit (Calbiochem) to analyze their HDAC (histone deacetylase) inhibition activities. The H-DAC fluorometric substrate, which comprises an acetylated lysine side chain, was incubated with extracted nuclear protein first. Deacetylation of the substrate sensitized 00 r the substrate, so that, in the second step, treatment with the Lysine Developer produced a \0 Ci fluorophore. The fluorophore can easily be analyzed by using a fluorescence plate reader.

As shown in Fig. 6, NBM-HD-1 can inhibit the HDAC activity in C6 glioma cells.

Inhibition of HDAC has been implicated in the induction of differentiation in cancer cells.

In this experiment, a well-known compound for HDAC inhibition, sodium butyrate, was used as a positive control. The lower fluorescence unit showed higher HDAC inhibition activity in the experimental group. The results indicated that NBM-HD-1 significantly inhibits HDAC activity.

Example 15 Inhibition of HDAC Activity and Change of the Morphology of Cancer Cells by the Compound of the Invention (NBM-IID-1) [0073] The human giloblastoma DBTRG-05MG cancer cells were cultured in RPMI medium 1640 (Gibco) supplemented with penicillin G, streptomycin sulphate, mM of L-glutamine and 10% fetal bovine serum (FBS Gibco), 10lmh/L sodium pyruvate (Gibco), and 1% NEAA (Gibco) at 37 5% CO, and 95% relative humidity.

For these experiments, the cells were seeded at a density of 3xl05 per well of 6-well plates. After 24 hours, the cells were treated with different concentrations of NBM-HID-1 and 4 mM sodium butyrate. The cells were observed and counted after 72 hours. As shown in Fig. 7, NBM-HD-1 significantly inhibited the growth of 05MG cancer cells and C, changed the morphology of the cells. In 05MG cells (Fig. after incubation with ,pg/mL (Fig. 5 ug/mL (Fig. and 10 pg/mL (Fig. of NBM- HD-I for 72 hours, the density of the cells reduced dramatically in comparison with that of the control group (Fig. The 05MG cells in the experimental group also 00 Schanged to become longer than those in the control group. The results of the cell counting (Fig. indicated that sodium butyrate could inhibit the proliferation of 05MG cancer cells, and so could NBM-HD-1. These results indicate that NBM-HD-1 can inhibit the growth of 05MG cells in a dose-dependent manner and change the morphology of cancer cells.

[0074] The breast cancer MCF-7 cells, were cultured in Dulbecco's modified Eagle's medium (DMEM Gibco) supplemented with penicillin G, streptomycin sulphate, 0.5 mM of L-glutamine and 10% fetal bovine serum (FBS Gibco) at 37 oC, CO2 and 95% relative humidity. The cells were seeded at a density of 3 x 105 per well of 6-well plates. After 24 hours, the cells were treated with different concentrations of compound NBM-HD-1 and 4mM sodium butyrate being used as the positive control.

The cells were observed after 48 hours, and counted after 96 hours. As shown in Fig. 8, NBM-HD-1 significantly inhibits the growth of MCF-7 cancer cells and changes the morphology of the cells. In Fig. after the cells were incubated with 2.5 ug/mL (Fig.

5 pg/mL (Fig. and 10 pg/mL (Fig. of NBM-HD-1 for 48 hours, the density of MCF-7 cells reduced dramatically in comparison with that of the control group (Fig. The morphology of the MCF-7 cells in the experimental group Q changed in comparison with that in the control group. Fig. and showed that 4mM sodium butyrate was able to inhibit"the growth of MCF-7 cells. The results of the cell Scounting (FIG. indicate that, sinilar to sodium butyrate, NBM-HD-1 inhibits cell growth. These results indicate that NBM-HD-1 can inhibit the growth of MCF-7 cancer cells in a dose-dependent manner and change their morphology.

00 [0075] MCF-7 cancer cells (1x106) in a 100-mm dish were treated with various CI concentrations of NBM-HD-1 2.5, 5, and 10pg/mL) or 4mM sodium butyrate for 72 Shours. The samples were prepared following the steps mentioned in [0063]. The DNA of the cells was then analyzed by FACScan cytometry (Becton Dickinson). As shown in Fig. 9, NBM-HD-I markedly inhibited MCF-7 cell growth via modulation of the cell cycle, arrested on the GO/GI phase6n a dose-dependent manner. The percentage of GO/GI phase increased from 74.46.to 92.55 in a dose-dependent manner. It was also found that NBM-l-D-1 significantly decreased both S and G2/M phases of the cells in a dose-dependent manner.

[0076] The cell cycle related to p21 mRNA expression was examined by RT-PCR.

The total RNAs were isolated from the treated MCF-7 cells and used in the RT reaction.

cDNA (Ill) was used as a template to amplify p21 gene by PCR. GAPDH was used as an internal control. As shown in Fig. 10, NBM-HD-I can increase the p21 mRNA expression in MCF-7 cancer cells. In this experiment, MCF-7 cells were treated with different doses of NBM-HD-I fo:24S'hours. The results indicated that NBM-HD-1 induced the expression of p21 in a dose-dependent manner.

[0077] Accumulation of hyperacetylated histone H4 was analyzed in the cell

O

c lysates by using Western Blotting and an antibody that binds to acetylated histone H4 (Upstate). The MCF-7 cancer cells were seeded at a density of lx106 per 10cm culture ^1dish. After 24 hours, the cells were treated with 10pg/mL of NBM-HD-1 or 4mM sodium butyrate for several hours. The HDAC inhibition by NBM-HD-1 was tested by 00 analyzing the degree of histone acetylation with a specific antibody for hyperacetylated Chistone H4. The sodium butyrate was used as a positive control. As shown in Fig. 11, the results with MCF-7 cells were similar to those with C6 glioma cells.

Example 16 Enhancing the Neurite Outgrowth by the Compound of the Invention

(NBM-IID-I)

[0078] The growth medium for the NSCs (neural stem cells) and cortical neurons was prepared by adding penicillin G, streptomycin and 0.5 mM of L-glutamine into a B- 27 supplemented neurobasal medium- (Gibco). The unborn fetus was taken out of the fetal sac in the abdominal cavity of a 17-day pregnant Wistar rat under anesthesia. The cerebral tissue was removed from the fetus and treated with 0.1% trypsin solution at °C for three minutes. After washing with PBS solution 3 times, the cells were dissociated by up and down mixing. The resulting solution was passed through a 70m Nylon cell strainer (Falcon) in order to obtain the filtrate that contained cerebral cells. The filtrate was centrifuged at 1000 rpm for 10 minutes and the supernatant was aspirated. The resulting pellet was resuspended in the growth medium prepared as stated above. The resulting suspension contained NSOs.

S[0079] The cells obtained from the suspension were cultivated in 6-well plates O2 c coated with 30 pg/ml of poly-D-lysine (Sigma) at the density of 75 cells/mm 2 The cells were cultured at 37 5% CO, and 95% relative humidity. The growth media contained 0.63 pg/mL of NBM-HD-1 and the growth media with 1 1 of DMSO was used as the control. The differentiated cells after cultivation were categorically called cortical 00 r- neurons.

C [0080] After cultivation for 6 days, live cells were observed by microscope. The lengths of the neurites of the neurons in 6 different fields were measured and averaged.

As shown in Fig. 12, NBM-H-D-I could promote the neurite outgrowth. In Fig. 12, the length of the neurites of the experimental group (Fig. 12(A)-b) is longer than that of the control group (Fig. After measuring the lengths of the neurites, the result indicated that the average length of-the neurites of the experiment group was greater than that of the control group Fig. 12(B)).

Example 17 Inhibition of Cancer Cell Growth by the Compound of the Invention (NBM-HD-2) [0081] MCF-7 cancer cells were seeded at a density of 3x105 per well in 6-well plates.

After 24 hours, the cells were treated with different concentrations of compound NBM-HD-2 and 4mM sodium butyrate being used as the positive control. The cells were observed and counted after 72 hours. As shown in Fig. 13, NBM-HD-2 significantly inhibits the growth of MCF-7 cancer cells and changes the morphology of the cells. In Fig. 13(A), after the cells were incubated with 2.5 pg/mL (Fig. 5 jg/mL (Fig. 7.5 jg/mL (Fig. 13(A)-e) and pg/mL (Fig. 13(A)-t) of NBM-HD-2 for 72 hours, the density of MCF-7 cells reduced Sdramatically in comparison with that of the control group (Fig. The morphology of the MCF-7 cells in the experimental group changed in comparison with that in the control group. Fig.

13(A) showed that 4mM sodium butyrate was able to inhibit the growth of MCF-7 cells. The results of the cell counting (FIG. 13(B)) indicated that, similar to sodium butyrate, NBM-HD-2 inhibited cell growth. These results indicate that NBM-HD-2 can inhibit the growth of MCF-7 00 cancer cells in a dose-dependent manner and change their morphology.

r- Example 18 Inhibition of Cancer Cell Growth by the Compounds of the Invention (NBM-HD-3) C [0082] MCF-7 cancer cells were seeded at a density of 3xl0 5 per well of 6-well plates.

To After 24 hours, the cells were treated with different concentrations of compound NBM-HD-3 and 4mM sodium butyrate, which was being used as the positive control. The cells were observed and counted after 72 hours. As shown in Fig. 14, NBM-H-D-3 significantly inhibits the growth of MCF-7 cancer cells and changes the morphology of the cells. In Fig. 14(A), after the cells were incubated with 2.5 pg/mL (Fig. 5 pg/mL (Fig. 7.5 <g/mL (Fig. 14(A)-e) and 10 /g/mL (Fig. 14(A)-f) of NBM-HD-3 for 72 hours, the density of MCF-7 cells reduced dramatically in comparison with that of the control group (Fig. The morphology of the MCF-7 cells in the experimental group changed in comparison with that in the control group.

Fig. 14(A) showed that 4mM sodium butyrate was able to inhibit the growth of MCF-7 cells.

The results of the cell counting (FIG. 14(B)) indicate that, similar to sodium butyrate, NBM-HD- 3 inhibits cell growth. These results indicate that NBM-HD-3 can inhibit the growth of MCF-7 cancer cells in a dose-dependent manner and change their morphology.

Claims (13)

1. 3- monocyclic or bicyclic ring, alkyl, alkylenyl, alkynyl, C 3 8 cycloalkyl, an unsaturated 5- to 10-membered monocyclic or bicyclic ring or a saturated or unsaturated r to 10-membered heterocyclic ring comprising at least one ring heteroatom selected from the group consisting of: N, O and S; R 5 is C 4 -16 alkyl or C 4 16 alkenyl wherein the alkyl or alkenyl is unsubstituted or 00 F substituted with one or more CI-, alkyl, 1OH-, halogen, CN, NO, N 3 NH 2 CHO, OR, SR 9 \O Ci NR 9 or COOR 9 R, is C 2 1 2 alkyl or C 2 1 2 alkenyl wherein the alkyl or alkenyl is unsubstituted or substituted with one or more CI. 6 alkyl, OH, halogen, CN, NO, N3, NH 2 CHO, OR, SR, or NR 9 or one of R 5 and R 6 is hydrogen, halogen or OH, while the other is C 416 alkyl or C4- 16 alkylene unsubstituted or substituted with one or more C 1 6 alkyl, OHI, NH 2 halogen, CN, NO or N 3 R 7 and Rs are each independently hydrogen, halogen, OH, NI-I 2 COOH, CHO, CN, NO, C 1 6 alkyl unsubstituted or substituted with OH, NI 2 COOH, halogen, CN, NO or CHO, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, O-alkynyl, S-alkynyl or N-alkynyl, or R 7 and R8 may together form a double bond, a C 3 6 cycloalkyl, or a 5- to membered heterocyclic ring comprising at least a heteroatom selected from the group consisting of N, O and S; R 9 is phenyl, C(=O)ORIW'. bhenzyl; and R' 0 is 01-OI, NHO-H, NH 2 C 1 -6alkyl, phbnyl or benzyl; -511- [13 [13 provided that when RI, RZ 2 R 3 and R 4 are 0141, 15 is not C 1-13 and R6 is not H 3 C or H;,or when RI, R 2 R 3 and R 4 are OH- and R5 is H, R 6 is 00H3 HH3 c-Inot o rHC and pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof. 2. The compound according to Claim I, wherein R, and R, are each independently OH, OCI- 6 alkyl, OC(=O)CI-6alkyl, 0-phenyl or O-benzyl or R, and R 2 together form dioxalene. 3. The compound according to Claim 2, wherein R, and are each independently 01-1, 0CV-1 3 OCH 2 CHI 3 OCH 2 CH 9 ICH 3 OC(=O)CH 3 ,O-phenyl or O-benzyl.
2. 4. The compound according to Claimn I, wherein R, and R 2 together form dioxalene. The compound according to Claim 1, wherein R 3 and R 4 are each independently 01-1, OCI 6 alkyl, OC(=-O)CI-balkyl, 0-phenyl or O-benzyl. 4. The compound according to Claim 1, wherein R 3 and R 4 are each independently 01-I, OCI-1 3 OCH 2 CH 3 OCIC1-1 2 CH 3 OC(=0)CH 3 ,O-phenyl or 0-benzyl. The compound according to Claim I1, wherein R 5 is H 3 H H 3 C B__H 3 1-13 H 3 C CI 4 aky1- C 1 4 alkyl 0 phenyl 0o 0 HO H H3 H 3 1-10 H- 3 C H1- 3 H, PhS H 3 C phenyl)" H O -56-
3. 6. The compound according to Claim 1, wherein R 6 is H 3 C HO 1- 3 C N 3 H 3 CJ 171 3 C OH PhSj 00 C1 CH 3 C 14 alkylO C,- 4 a11Yy1 0 0 phenyl 0 Phenyl 0o 0 0 ,or H
4. 7. The comnpound according to Claim 1, which is selected from the group consisting of: -57- 00 H 3 H 3 Ac AcO 0 H 3 C AOce 3 C CI-1 3 OHc 0 113 113 Me H 3 Cj m 0" 11 3 C, I CH 3 Oln 0 -131-3B -58- I-1 3 c, H- 2 N MeO 0 PhS liob CH 3 OMe 0 00 ON 0 HO o 2007216781 14 Sep 2007 OCH 3 H 3 HO I CH 3 OH 0 CH 3 CH 3 H 3 CN CZ< HOX CH 3 OHr0 9CH 3 -62- CH 3 CH 3 OCH 3 HOI ~OCH 3 H 3 C H 3 CO 0 CH 3 OH 0
5. 8. The compound according to Claim 1, which is selected from the group consisting of: ,OCH 3 H 3 C H 3 C- H 3 CO 0-, H 3 C HO:' 00 CH 3 OH 0 CH 3 CH 3 OCH 3 I N. I CH 3 CK1 H 3 C H 3 CON HONI -i CH 3 OH '0ad CH 3 CH 3 OCH 3 H 3 C, CH 3 OH 0
6. 9. A stereoisomner of the compound of formula as defined in Claim 1, which is represented by the following form-ula (11): R 4 R 7 00 wherein R I, R 2 R 3 4 R 5 Rs, R 7 and R 8 are defined as those in formula IND 1O A pharmaceutical composition comprising the compound of any one of Claims I to 9 or phanraceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof as an active ingredient and a pharmaceutically acceptable carrier.
7. 11. Use of the compound of any one of Claims 1 to 9 or pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof in the manufacture of a medicament for inhibiting histone deacetylase (HDAC) in a subject.
8. 12. Use of the compound of any one of Claims 1 to 9 or pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof in the manufacture of a medicament for treating tumor or cell proliferative disease in a subject.
9. 13. Use of the compound of any one of Claims 1 to 9 or pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof in the manufacture of a medicament for enhancing the neurite outgrowth in a subject. 66
10. 14. Use of the compound of any one of claims 1 to 9 or pharmaceutically acceptable salts, stereoisomers, enantiomers, prodrugs and solvates thereof in the Smanufacture of a medicament for treating neurodegenerative diseases and human spinal muscular atrophy (SMA) in a subject.
11. 15. A method for inhibiting histone deacetylase (HDAC) in a subject, comprising administering to a subject in need thereof an effective amount of a compound of any one 0 0 of claims 1 to 9 or a pharmaceutical composition of claim r"- IND 16. A method for treating tumor or cell proliferative disease in a subject, comprising administering to a subject in need thereof an effective amount of a compound to of any one of claims 1 to 9 or a pharmaceutical composition of claim
12. 17. A method for enhancing the neurite outgrowth in a subject, comprising administering to a subject in need thereof an effective amount of a compound of any one of claims 1 to 9 or a pharmaceutical composition of claim
13. 18. A method for treating neurodegenerative diseases and human spinal muscular atrophy (SMA) in a subject, comprising administering to a subject in need thereof an effective amount of a compound of any one of claims 1 to 9 or a pharmaceutical composition of claim Dated 13 September, 2007 Naturewise Biotech Medicals Corporation Patent Attorneys for the Applicant/Nominated Person SPRUSON FERGUSON