AU2004267873A1 - Compositions for veterinary and medical applications - Google Patents

Compositions for veterinary and medical applications Download PDF

Info

Publication number
AU2004267873A1
AU2004267873A1 AU2004267873A AU2004267873A AU2004267873A1 AU 2004267873 A1 AU2004267873 A1 AU 2004267873A1 AU 2004267873 A AU2004267873 A AU 2004267873A AU 2004267873 A AU2004267873 A AU 2004267873A AU 2004267873 A1 AU2004267873 A1 AU 2004267873A1
Authority
AU
Australia
Prior art keywords
formula
compound
dialdehyde
och
composition according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU2004267873A
Other versions
AU2004267873C1 (en
AU2004267873B2 (en
Inventor
Betty Jin
Wen-Yang Wu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Irp Health Pty Ltd
Original Assignee
Australian Biomedical Co Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2003904654A external-priority patent/AU2003904654A0/en
Application filed by Australian Biomedical Co Pty Ltd filed Critical Australian Biomedical Co Pty Ltd
Priority to AU2004267873A priority Critical patent/AU2004267873C1/en
Priority claimed from PCT/AU2004/001151 external-priority patent/WO2005020997A1/en
Publication of AU2004267873A1 publication Critical patent/AU2004267873A1/en
Application granted granted Critical
Publication of AU2004267873B2 publication Critical patent/AU2004267873B2/en
Publication of AU2004267873C1 publication Critical patent/AU2004267873C1/en
Assigned to IRICCORGPHARM PTY LTD reassignment IRICCORGPHARM PTY LTD Request for Assignment Assignors: AUSTRALIAN BIOMEDICAL COMPANY PTY LTD
Assigned to IRP Health Pty Ltd reassignment IRP Health Pty Ltd Request to Amend Deed and Register Assignors: IRICCORGPHARM PTY LTD
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

Links

Landscapes

  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Description

WO 2005/020997 PCT/AU2004/001151 1 Compositions for Veterinary and Medical Applications Background to the Invention 5 Gastrointestinal ("GI") function disorders are caused by the invasion of pathogens, post treatment of broad-spectrum antibiotics, improper diet, stressful lifestyle and other causative factors. They are very common diseases, with conditions such as Irritable Bowel Syndrome (IBS) presenting in as much as 20% of the adult population in the USA. In Canada, IBS is second only to the 10 common cold as the leading cause of time absent from school and work. So far, there is no effective medicine for the treatment IBS and Inflammatory Bowel Disease (IBD). Desirably, compositions for therapeutic purposes should meet the following criteria: * Non-toxic, safe to use. 15 e Not inhibiting or adversely affecting probiotic bacteria in the gut. 0 Preferably very poor oral bioavailability (no systemic effects). * Not causing bacterial resistance. * Anti-inflammatory. * Anti-diarrhoea, antisecretory. 20 e Anti-motility. * Strengthening immunity. * Neutralizing toxins. This invention seeks to provide compositions and treatments for GI functional 25 disorders which meet one or more of the above criteria. In humans, the gastrointestinal (GI) tract with an area of 300-400m 2 is the second largest surface connecting the body with the outside world. With consumption of 1 - 2kg of food every day, the GI immune system is presented 30 with the threat of ingested poisons and pathogens together with an enormous variety of harmless antigens. The GI tract digests food and absorbs the nutrients that are beneficial to the body, while eliminating components that pose a potential risk to health. A large portion of the body's immune system is located in the GI wall and the mesenteric lymph nodes, called gut-associated lymphoid WO 2005/020997 PCT/AU2004/001151 2 tissue (GALT) system. GI secretions are rich in antimicrobial factors such as lactoferrin and lysozyme and other factors, like important growth and mucosa healing factors such as epidermal growth factor (EGF). The mucosa of the gut is normally covered by a unique protective layer of mucus and is colonized by 5 microflora, which perform a key function in the regulation of the GALT system. The mucus serves to a large extent as a matrix for the indigenous protective flora. The intestines contain about 1kg probiotic bacteria. The roles of these bacteria are to maintain the healthy ecology in the GI tract, synthesizing 10 vitamins, hormones, and other important factors, and to help to break down complex proteins and fiber into smaller molecules that can be absorbed by the mucosal cells.' Pathogenic bacteria in the intestinal tract are an important aspect in the GI 15 functional disorders both as a causative factor and as a symptom. Other disorders can. actually cause the GI tract to lose probiotic bacteria and allow pathogenic bacteria growth, eventually, resulting in changes to the intestinal ecology and further exacerbation of the GI functional disorders. 20 For the treatment of GI functional disorders it is important to restore healthy ecology. This invention relates to the treatment of GI functional disorders and related conditions including IBS and IBD including colitis, Crohn's disease and coeliac disease. The treatment is expected to improve intestinal health and reduce symptoms including constipation, flatulence and diarrhoea. It acts as a 25 GI cleanser, strengthening the immune system, inhibiting and removing the pathogenic bacteria, and helping to restore a healthy ecology in the intestinal tract. The resulting healthy digestion system will support the healthy state of the body and healthy weight gain in animals including humans. 30 Berberine is an isoquinoline quaternary alkaloid derived from a number of species of the barberry plant including Berberis aristata and Coptis chinensis 21 Structural analogues of berberine have been isolated from extracts of the Chinese medicinal plant, Acangelisia gusanlung 31 which has been used for over 2000 years in traditional Eastern medicine to treat gastro-enteritis and WO 2005/020997 PCT/AU2004/001151 3 secretory diarrhoea[ 4 ] and is also effective in the prevention and the treatment of animal models of diarrhoea.
5
-
71 Berberine possesses antimicrobiall 4 l, anti motility 8 1 and anti-secretory properties. 9 ~" 1 Thus, several mechanisms may contribute to the therapeutic usefulness of berberine. Berberine has been used 5 as an anti-diarrhoea drug at dose range of 100mg-300mg t.i.d. in adult (6mg-18mg/kg/day) in China.' 21 Finally the apparent permeability coefficient (Papp) of berberine across the intestinal tissue was of the order of 10-7 cm/s 131 , typical of the values of poorly absorbed compounds, and reflected by poor bioavailability in vivo. This poor oral bioavailability causing poor systemic 10 absorptions should offer the benefit of safe use of the oral administration of berberine. Certain saturated lower dialdehydes also possess antibacterial activity toward sulfate-reducing bacteria. 141 Furthermore, alcoholic sporicidal compositions 15 containing similar saturated lower dialdehydes were taught.r 51 Also it is known that water-soluble dialdehyde starch can be incorporated into chewing gum compositions as a cariostatic agent 16 1 , a water insoluble dialdehyde polysaccharide being applied in medium at a concentration of at least about 0.1 weight per cent to inhibit the bacterial growth.
71 Synthetic polymeric 20 dialdehydes such as poly-(2-propenal, 2-propenoic acid) have been used in the treatment of gastrointestinal diseases. However, since the antimicrobial activities of these compounds are very weak, very high doses for treatment, such as 500-2500mg/kg body weight/day are required. [18] 25 After searching and screening it has been found that compositions including compounds of Formula (1) below, (particularly berberine chloride), and compounds of Formula (2) below, (particularly oxidized cellulose), are each useful for the treatment of the GI functional disorders and related conditions as well as for a method of promoting weight gain in animals including humans. 30 One of the most important findings of the invention is the existence of a synergic effect obtained by using combination of the compounds of Formula (1) and compounds of Formula (2) (see Example 4 and Example 8, below). This synergic effect provides the possibility of using lower dosage of the compounds, thereby achieving higher safety and economy.
WO 2005/020997 PCT/AU2004/001151 4 Disclosure of the Invention 5 In one aspect, the present invention relates to a composition including a compound of Formula (1) and/or a polymeric dialdehyde, for the treatment of gastrointestinal (GI) functional disorders and related conditions, including Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD), Colitis, Crohn's disease and coeliac disease; and/or for promoting weight gain in 10 animals, where Formula (1) comprises: 3 4 6 2 R R H-3CO +NR Formula (1) H3CO OR' R1 R 7 R9 Rio where R', R 2 , R 7 , R 8 , R 9 , and R 1 0 may be the same or different and are selected from H, CH 3 , OH, OCH 3 , C 2
H
5 , OC 2
H
5
OCH
2 Ph, OCH 2 PhNO 2 , F or Cl; 15 R 3 , R 4 , R 5 , R' may be the same or different and are selected from H, CH 3 ,
OCH
3 , C 2
H
5 , OC 2
H
5
OCH
2 Ph, OCH 2 PhNO 2 , F or Cl, or
R
5 and R 6 are the same or different and are selected from H, CH 3 , OCH 3 , C 2
H
5 ,
OC
2
H
5
OCH
2 Ph, OCH 2 PhNO 2 , F or Cl and R 3 and R 4 together are =0, or
R
4 and R' are the same or different and are selected from H, CH 3 , OCH 3 , C 2
H
5 , 20 OC 2
H
5
OCH
2 Ph, OCH 2 PhNO 2 , F or Cl and R 3 and R 5 together form a double bond or are =0, or
R
3 and R 4 , are the same or different and are selected from H, CH 3 , OCH 3 , C 2
H
5 ,
OC
2
H
5
OCH
2 Ph, OCH 2 PhNO 2 , F or C and R 5 and R 6 together are =0;
R
1 1 and R 12 together form =CH 2 , or R 11 and R 12 may be the same or different 25 and are independently selected from the group consisting of H, CH 3 , CH 2
CH
3 and CH 2
CH
2
CH
3 ; and X is selected from the group consisting of Cl, Br, SO 4 , I and R 13 COO, where
R
13 is CH 3 or poly acids.
WO 2005/020997 PCT/AU2004/001151 5 Preferably, R', R 2 , R 3 , R 4 , R 5 , R',R', R 8 , R 9 and R 1 0 , are hydrogen, R" and R 12 together form H 2 C= and X is Cl so that Formula (1) is berberine chloride. 5 The polymeric dialdehyde may be chosen from a wide range of suitable compounds. For example poly-(2-propenal, 2-propenoic acid) may be combined with a compound of Formula (1) to form the compositions of this invention. Preferably, the polymeric aldehyde is a dialdehyde polysaccharide. Particularly preferred dialdehyde polysaccharides have a formula in accordance 10 with Formula (2): HO - R R4 Rr Rp - R -Rr - H 1 1 r 1 n~ nR n Formula (2) 15 wherein each of the monomers RP and Rr, are independently selected from the group consisting of: 14 --- CH 2 0 0 OH OH H OH O.._ OH H OH (A) and (B) and (C) and each monomer Rq is independently selected from the group consisting of: 20 14 14 R R O0 --- CH2 0 0 O O (D) and (E) and (F) and WO 2005/020997 PCT/AU2004/001151 6 ~ CH2 OO HO1 O -- (G) wherein each R 14 may be the same or different and is independently selected from the group consisting of CH 2 OH, COOH, CH 2 0CH 2 COOH and CH 2 0R 5 , 5 where R 1 5 is selected from the group consisting of CH 2
CBH
4 COOH, C 6
H
4 COOH and CH 2
(CH
2 )yCOOH where y=1 to 20; wherein pi -- Pn may be the same or different and are each independently selected from the range 0 to n; q1 -- qn may be the same or different and are each independently selected from the range 1 to m, preferably from the range 2 10 to m; r 1 - r, may be the same or different and are each independently selected from the range of 0 to n, n is an integer greater than 0 and m is an integer greater than 1; and wherein qi+-___ x 100% 30% p1+-+pn+q+-+q+r1+-+rn 15 In one preferred embodiment, each RP and Rr are (A) and Rq is (D), so that the polymeric dialdehyde is oxidised cellulose. In this case, it is also preferred that
R
14 is CH 2 OH or CH 2 0CH 2 COOH. 20 In another preferred embodiment, each RP and Rr are (B) and Rq is (E), so that the polymeric dialdehyde is oxidised starch or dextrin. In another preferred embodiment, each RP and Rr are (C) and each Rq is (F) or (G) so that the polymeric dialdehyde is oxidised dextran. 25 In each case, it is preferred that: q _+--_+ _ _ x 100% 40% pl+---+pn+q1+---+q+r1+-+rn 30 and particularly preferred that: 40%:5 s q+- _+ _ _ x 100%5 60% p1+-+pn+q1 +-+qa+r1 +-+rn WO 2005/020997 PCT/AU2004/001151 7 that is, the oxidised cellulose, starch, dextrin or dextran is from 40% to 60 % oxidised. The compounds of Formula (2) preferably have a molecular weight of from 5 1,000 to 1,000,000. More preferably, they have a molecular weight of from 10,000 to 750,000. Where the compounds of Formula (2) are water-insoluble, they preferably have a particle size of from 51_ to I 00[t, more preferably from 5L to 30pL. For example, the diameter of microcrystalline oxidized cellulose is about 2 0Vt. 10 Generally, the oxidized rate of the Formula (2) is from 30-100%, preferably 40-100%, more preferably 40-60%. It was found that the compounds of the Formula (2) were non-toxic by oral administration. In particular, the oxidized celluloses are relatively stable in the GI tract. Their high molecular weights 15 prevent them being absorbed by gut. Their polydialdehyde functional groups interact and neutralize toxins. The compounds of the Formula (2) possess an anti-constipation effect. The compounds also promote ulcer healing. The compounds do not adversely affect the growth of probiotic bacteria in gut. 20 Animals to which the composition may be administered include: primates including humans, birds including poultry, ungulates including cattle, sheep, horses, cervidae and swine, fish including crustaceans and molluscs, reptiles, rodents, canines and felines. 25 In another embodiment, the invention comprises using compounds of Formula (1) and compounds of Formula (2) in the manufacture of a medicament for the treatment of GI functional disorders and related conditions including Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD) including Colitis, Crohn's disease and coeliac disease; and for promoting weight gain in animals 30 including humans. In another embodiment, the invention comprises a method of treating gastrointestinal disorders including Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD), colitis, Crohn's disease and coeliac disease, WO 2005/020997 PCT/AU2004/001151 8 including administering to an animal, including a human, suffering from a gastrointestinal disorder, effective amounts of a compound of Formula (1) and/or polymeric dialdehyde. Preferably, the method of treatment comprises administering effective amounts of a compound of Formula (1) and polymeric 5 dialdehyde in conjunction. In different embodiments of the invention treatment may comprise administering the compound of Formula (1) and the polymeric dialdehyde sequentially or simultaneously. In a preferred embodiment the compound of Formula (1) is combined with the polymeric dialdehyde to form a composition which is then administered. Preferably the polymeric dialdehyde-is 10 a compound of Formula (2), more preferably oxidised cellulose, especially oxidised cellulose having an oxidation level of from 40% to 60%. The required dose of the composition containing Formula (1) and a polymeric dialdehyde is generally less than 50% of those separately using individual 15 components. For example, the dose of beberine is 6mg-18mg/kg/day for adult. The use of 40% oxidized cellulose is 250mg-750mg/kg/day. However, when using combination of berberine and 40% oxidized cellulose the required dose is berberine 0.5mg-6mg/kg/day plus 40% oxidized cellulose 5mg-200mg/kg/day. Preferably, in humans, a dose of berberine 0.5mg-3mg/kg/day plus 40% 20 oxidized cellulose 5mg-80mg/kg/day is used. In addition, effective amounts of a compound of Formula (1) and polymeric dialdehyde may be administered in conjunction to promote weight gain in animals, including humans. In this context the compounds may be 25 administered as feed additives. Accordingly, the invention includes modified foods containing from 0.1 to 50 ppm of a compound of Formula (1) and from 1 to 400 ppm of a polymeric dialdehyde. Preferably, the modified food contains from 2 to 10 ppm of a compound of Formula (1) and from 10 to 200 ppm of a compound of Formula (2); more preferably Formula (1) is berberine chloride 30 and Formula (2) is oxidized cellulose. It is envisioned that the compound of Formula (1) and the polymeric dialdehyde may be provided separately, rather than as a composition. The present invention also comprises a kit including a quantity of a compound of Formula (1) WO 2005/020997 PCT/AU2004/001151 9 and a quantity of a polymeric dialdehyde for the treatment of gastrointestinal (GI) functional disorders and related conditions including Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD) including colitis, Crohn's disease and coeliac disease; and for promotion of weight gain in animals 5 including humans. Preferably the weight ratio of the quantity of compound of Formula (1) to the quantity of polymeric dialdehyde ranges from 1:1 to 1:100, more preferably from 1:10 to 1:40. It has also been found that berberine selectively inhibits pathogenic bacteria 10 such as Staphylococcus aureus, Streptococcus Group B, Vibrio cholerae, Clostridium perfringens, Candida albicans, but does not inhibit beneficial bacteria (probiotics) such as Lactobacillus plantarum at <500 Dg/ml (see Table 1). 15 Table 1 Antimicrobial Activities of berberine, oxidized cellulose and Erythromycin Microorganism Minimum Inhibitory Concentration (Og/ml) Berberin Oxidized Erythromyci e cellulose n Staph. Aureus ATCC 29213 125 >1,000 <0.625 Strep. Group B MCR1 27 62.5 >1,000 <0.625 E. coli HS >500 >1,000 >10 V. cholerae 6239 125 >1,000 >10 C. albicans ATCC 14053 62.5 >1,000 >10 Cl. perfringens ATCC 1124 125 >1,000 1.25 L. plantarum 500 >1,000 <0.625 Pathogenic bacteria like Escherichia Coli lost their filaments and were unable to attach on the wall of intestine after being treated with berberine at 50g/ml, 20 which is a much lower concentration than MIC (Minimum Inhibition Concentration). However, at this concentration, berberine did not affect probiotic bacteria such as Lactobacilli. This specific property of berberine offers an important aspect as a GI tract cleanser, since it can selectively remove pathogenic bacteria from the gut without affecting the inhabitations of the WO 2005/020997 PCT/AU2004/001151 10 probiotic bacteria in GI tract, and without exerting excessive drug pressure on the bacteria, which may lead to drug resistance. The currently available broad spectrum antibiotics indiscriminately inhibit both 5 pathogenic and probiotic bacteria in gut. Antibiotic treatment often causes GI function disorders. Animals and plants have coexisted with microbes throughout their evolution, sometimes to their mutual benefit, often in an antagonistic relationship. Berberines are an ancient and pervasive component of the innate defence mechanisms; they have developed to control the natural flora and 10 combat pathogens. They do not target specific molecular receptors on the microbial surface. This characteristic may avoid the problem of inducing bacterial resistance occurred to most antibiotics. It was also found that berberine could induce the interleukine-12 (IL-12) (pro-antiinfectious) and inhibit the production of IL-8 (pro-inflammatory). This antiinflammatory property 15 strengthening the host immunity is also very useful for the treatment of IBD and IBS. In addition to use of the compounds of Formula (1), it has been found that compounds of Formula (2) not only possess antibacterial activity, but are also 20 able to neutralize toxins and act as an antioxidant. Accordingly, the research leading to the present invention also discloses the invention of treating Irritable Bowel Syndrome, Inflammatory Bowel Disorder, Colitis, Crohn's disease and ceoliac disease including administering an effective 25 amount of a compound of Formula (1) or of Formula (2) to an animal, including a human, in need thereof. The invention also provides a method of promoting weight gain in animals including humans including administering an effective amount of a compound of 30 Formula (1) and/or Formula (2). Hereinafter, GI tract cleansing compositions that include compounds of Formula (1) and compounds of Formula (2), and/or the pharmaceutically acceptable derivatives thereof may be referred to as GILAX cleansers. The amount of WO 2005/020997 PCT/AU2004/001151 11 GILAX-cleanser required for use in treatment will vary with the nature of the condition being treated and the age condition of the animal including human patients, and will ultimately be at the discretion of the attendant veterinarian or physician. 5 In general, a GILAX-cleanser comprises the compounds of Formula (1) and the compounds of Formula (2) at a weight ratio of 1:1-100, preferably at a weight ratio of 1:10-40. For example, for humans, the dose of GILAX-cleansers (for instance, berberine chloride and oxidized cellulose at a weight ratio of 1:10) 10 may be 275 -1650mg/day orally. The dose taken is according to the GI functional disorder condition. It is recommended for an adult to take 550mg once daily for IBS or post antibiotic GI functional disorder, 275mg x 2 daily for IBD, 550mg x 4 daily for acute diarrhoea or other severe conditions. 15 In animal farming, such as the poultry industry, GILAX-cleansers may be used as food additives to promote growth. The composition is preferably 1-20ppm of berberine plus 10-400ppm of oxidized cellulose; more preferably, 5ppm of berberine plus 50ppm of oxidized cellulose. 20 In swine industry, GILAX-cleansers can be used to protect piglets from diarrhoea. A composition including 0.5mg-6mg of Formula (1) (berberine)/kg/day and 50mg-200mg of Formula (2) (oxidized cellulose)/kg/day may be used. 25 A GILAX-cleanser composition is preferably formed by combining the compounds of Formula (1) and the compounds of Formula (2) with one or more other ingredients, for example: vitamins, antibiotics, antiseptic agents, surfactants, antidiarrheal agents, anti-constipation agents, enzymes, (especially digestive enzymes), probiotic bacteria, herbs, vaccines, ulcer healing agents 30 (e.g. gibberellins, glucans). In accordance with the invention, a pharmaceutical formulation including the GILAX-cleansers or pharmaceutically acceptable derivatives thereof may also contain one or more pharmaceutically acceptable carriers and, optionally, other WO 2005/020997 PCT/AU2004/001151 12 therapeutic and/or prophylactic ingredients. The carriers must be acceptable in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof. 5 Pharmaceutical formulations may be in any form suitable for administration to the gastrointestinal tract, including those suitable for oral, and rectal administration. While it is possible that for use in therapy, the GILAX-cleansers may be administered as the raw chemical(s), it is preferable to present the active ingredient(s) as a pharmaceutical formulation. The formulation may, 10 where appropriate, be conveniently presented in discrete dosage units and may be prepared by any of the methods known in the art of pharmacy. Preferably, the methods include the step of bringing into association the active compound(s) with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulations. 15 Pharmaceutical formulations suitable for oral administration may be presented as discrete units such as capsules, cachets, or tablets each containing a predetermined amount of the active ingredient(s); as a powder or granules; a solution, a suspension or as an emulsion. The active ingredient(s) may also be 20 presented as a bolus, electuary or paste. Tablets and capsules for oral administration may contain conventional excipients such as binding agents, fillers, lubricants, disintegrants, or wetting agents. The tablets may be coated according to methods known in the art. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups 25 or elixirs, or may be presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, emulsifying agents, non aqueous vehicles (which may include edible oils), or preservatives. 30 For administration to a gastrointestinal ulcer such as peptic ulcer, the compounds of GILAX-cleanser or pharmaceutically acceptable derivatives thereof may be administered by any of the methods and formulations employed in the art of administration to the gastrointestinal tract.
WO 2005/020997 PCT/AU2004/001151 13 Where desired, formulations adapted to give sustained release of the active ingredient may be employed. The compounds of the GILAX-cleansers may also be used in combination with 5 other therapeutic agents, for example, anti-infection agents, such as antibiotics, or ulcer healing agents such as gibberellins, glucans, growth factors (EGF), and/or probiotic bacteria such as Lactobacillus plantarum. The combinations mentioned above may conveniently be presented for use in 10 the form of a pharmaceutical formulation and thus such formulations including a combination of compounds of Formula (1) and of Formula (2) as defined above, together with a pharmaceutically acceptable carrier therefore comprise a further aspect of the invention. 15 When the compounds of GILAX-cleansers are used with a second therapeutic agent active in the treatment of GI functional disorders and related conditions, the dose of each compound may either be the same as or differ from that employed when each compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art. 20 The compounds of GILAX-cleansers and their pharmaceutically acceptable derivatives may be prepared by any methods known in the art for the preparation of compounds of analogous structure. 25 Examples The invention will now be discussed with reference to examples. The examples are by way of illustration only and should not be construed as any limitation on the scope of the invention. 30 Example 1. Preparation of a compound of Formula (1), berberine chloride. The root and bark of berberines vulgaris (1.5kg, grounded) was refluxed with ethanol (IOL x 2). The ethanol extracts was filtered and evaporated under reduced pressure to afford a brownish oil. This residue was dissolved in a warm 0.1M HCI solution (IOL x 2), filtered, and the filtrate was vacuum evaporated to WO 2005/020997 PCT/AU2004/001151 14 about 1L, then stirred at room temperature overnight. The yellow precipitates were collected and washed with cold water, then redissolved in boiling water (IOL x 2), cooled to room temperature to afford berberine chloride (44.5g) as yellow crystalline powder after filtration, washing with water, and air-drying. 5 Analysis of the crystals using NMR produced the following results: 1 H-nmr (CD 3 0D) x (ppm) 3.16 (t, 2H), 4.02 (s, 3H), 4.15 (s, 3H), 4.88 (t, 2H), 6.12 (s, 2H), 7.03 (s, 1H), 7.74 (s, 1H), 7.95 (d, AB, 1H), 8.15 (d, AB, 1H), 8.89 (s, 1H), 9.83 (s, IH). 10 Example 2. Preparation of a compound of Formula (2), 40% oxidized cellulose. To a stirring solution of periodic acid (140.7g, 0.6175 mole) in water (1.08L) at 15 pH<0.5 was added in portions microcrystalline cellulose (particle size of -20 in diameter) (250g, 1.54 mole) at <300C over a period of 2 hours. The whole mixture was stirred at 30~32'C for 4 hours, then at room temperature for 16 hours. To this resulting reaction mixture was added a 5% sodium hydroxide solution (-480ml) to adjust the solution pH to 5-6. The suspension was filtered; 20 the solid was washed with water (2L x 4) until the filtrate on KI-starch test paper showed the absence of the oxidant. The solid was then washed with acetone (0.5L) and air dried to afford 40% oxidized cellulose as a white powder (215g, 87%). 25 Example 3. Preparation of a compound of Formula (2) 40% oxidized water soluble cellulose. To a stirring solution of carboxymethyl cellulose (MW 250,000, DS=0.7) (1g, 30 6.17 mmole) in water (25ml) in an ice-bath, a solution of sodium periodate (532.5mg, 2.48 mmole) in water was added dropwise over a period of 2 hours. The reaction mixture was stirred at 5-10 0 C for 16 hours, then dialysed against water in a dialysis tube (the cut-off MW-10,000) for 48 hours. The solution was WO 2005/020997 PCT/AU2004/001151 15 then freeze dried to afford 40% oxidized water soluble cellulose as a white powder (813mg, 81.4%). Example 4. 5 Pilot chicken experiment. Male day old chickens (white Leghorn E New Hampshire) were fed Barastoc chicken crumbles, which contain zinc bacitracin and D.O.T. (3,5-dinitro-ortho tuluamide) and antioxidants ethoxyquin and B.H.T. for 13 days. On day 14, they 10 were randomly divided into six groups of five chickens each, with the average weight of chickens from each group varying from 121.4g to 124.2g (2.3% variance betiveen all the groups). The chickens were then fed with same basic growers pellets, which did not contain any antibiotics but do contain D.O.T., with different food additives shown as follows: 15 Group A: feed additive is 50ppm berberine chloride. Group B: feed additive is 10ppm berberine chloride. Group C: feed additive is 200ppm 40% oxidized cellulose. Group D: feed additive is 100ppm 40% oxidized cellulose. 20 Group E: feed additive is 5ppm berberine chloride + 50ppm 40% oxidized cellulose. Control: no feed additive. The chickens were fed for another 23 days. Observation was carried out on the 25 feed intake, weight gains, colour of combs (a red coloured comb indicates a healthy state of the bird), and mortality. There were no fatalities in the experiment. There were no significant differences in the amount of feed intakes among the groups. The statistical analysis was performed two-tailed at a significance level of p=0.05. The results were shown in Table 2.
WO 2005/020997 PCT/AU2004/001151 16 Table 2 Synergic effect of the composition of the compound of Formula (1) (berberine chloride) and the compound of Formula (2) (40% oxidized 5 cellulose) on chicken growth Experimental Red Combs Growth rate Group (Number of red comb/total number of chickens compared to that of Day Day Day Day Day Day Day the control on day 36 14 17 21 24 26 32 36 A 0/5 1/5 1/5 3/5 3/5 4/5 4/5 106.36% (p= 0 .0 5 ) B 0/5 0/5 2/5 2/5 3/5 4/5 5/5 101.79% C 0/5 1/5 1/5 1/5 4/5 4/5 5/5 101.74% D 0/5 0/5 0/5 0/5 1/5 2/5 3/5 99.82% E 0/5 1/5 1/5 1/5 2/5 5/5 5/5 108.11% (p=0.05) Control 0/5 0/5 0/5 0/5 0/5 1/5 1/5 100.00% There was no residue of either berberine or oxidized cellulose found in the meat of chickens. 10 Example 4, demonstrates the synergic effect of the invention. From the results, it is clear that the composition of 5ppm berberine plus 50ppm oxidized cellulose gave the best growth promotion compared to 20ppm berberine or 10ppm berberine or 200ppm oxidized cellulose, or 100ppm oxidized cellulose alone. 15 This synergic effect provides the possibility of using lower dosages of the compounds, thereby achieving higher safety and economy. Example 5. Preparation of GILAX-cleanser. 20 a) Berberine chloride 50mg mixed with 40% oxidized cellulose 500mg was packed in a capsule for oral administration for adult for the treatment of IBS or post antibiotic GI functional disorder or diarrhea. b) Berberine chloride 25mg mixed with 40% oxidized cellulose 250mg was 25 packed in a capsule for oral administration for the treatment of IBD.
WO 2005/020997 PCT/AU2004/001151 17 Example 6. Combination of GILAX-cleanser with probiotic bacteria for the treatment of IBS and IBD. 5 GILAX-cleanser from example 5 may be used sequentially or simultaneously with the preparation of probiotic bacteria. Probiotic bacteria capsule or tablet, protected from air, contains 0.5-1 x 1010 CFU/capsule or tablet. Its composition is shown as follows: Bifidobacterium bifidum 30% Bifidobacterium longum 20% Lactobacillus plantarum 10% Lactobacillus bulgaricus 10% Lactobacillus salivarius 10% Lactobacillus acidophilus 20% 10 Example 7. Compound of Formula (2), 40% oxidized cellulose as food additive to promote chicken growth. 15 The chicken experiment was set up substantially as described in example 4, but with 6 chickens in each of the experimental and control groups. However, the feed additive was 1,000ppm of 40% oxidized cellulose. The growth rate of the experimental group of six chickens was 3.86% (p=0.05) higher than the six chickens in the control group. 20 Example 8. Synergic effect of the compound of Formula (1) and the compound of Formula (2) on the treatment of IBD on mouse model. 25 hFUT1 mouse model of colitis was used. It provides insights into the pathogenesis of Inflammatory Bowel Disease (IBD). These mice do not develop disease in a germ free environment. Immune dysfunction contributing to IBD in humans includes abnormal T cell reactivity and a loss of tolerance to gut WO 2005/020997 PCT/AU2004/001151 18 bacteria. The compositions of Formula (1) and Formula (2) were given orally to mice to see the effectiveness on the altering the gut flora and lessening the severity of the colitis. The positive results indicated the compositions of Formula (1) and Formula (2) may have a therapeutic role in human IBD. 5 A total five groups with ten mice per group were used as follows. 1) Control (without treatment). 2) Treated with compound of the Formula (1) (berberine chloride), 13.5mg /kg/day (o.s.). 3) Treated with compound of the Formula (2) (40% oxidized cellulose), 10 114mg/kg/day (o.s.). 4) Treated with composition of the Formula (1) (berberine chloride) (6.7mg/kg/day) and the Formula (2) (40% oxidized cellulose) (57mg/kg/day) (o.s.). 5) Treated with composition of the Formula (1) (berberine chloride) 15 (13.5mg/kg/day) and the Formula (2) (114mg/kg/day) (o.s.). The results are shown in Table 3. Table 3 Synergic effect of the composition of the compound of Formula (1) 20 (berberine chloride) and the compound of Formula (2) (40% oxidized cellulose) on IBD in mice Group No Survival rate after 56 days treatment 1 3/10 2 5/10 3 5/10 4 9/10 5 9/10 25 WO 2005/020997 PCT/AU2004/001151 19 Example 9. Tolerance dose testing of the compound of Formula (1) (berberine chloride) and the compound of Formula (2) (40% oxidized cellulose) on Balb-C mice. 5 30 male and 30 femal Balb-C mice were divided into groups of 10. 10 male and 10 female mice were in each of three groups as follows; 1) Control (without compound). 10 2) Oral administration (gavaged) with berberine chloride 250mg/kg/day for 14 consecutive days. 3) Oral administration (gavaged) with 40% oxidized cellulose 1330mg/kg/day for 14 consecutive days. 15 All mice in the experiment survived and they were all healthy and no significant difference between the groups. The histological observation on the tissue slides (liver, kidney, lung, intestine) from the tested mice showed no abnormality. 20 Example 10. Evaluation of GILAX as feed additive for pig health after weaning. Most pig farms run in a traditional continuous flow system with some co 25 mingling of weans, limited age group separation and routine pig flow between site areas. These factors can lead to incidents of diarrhea after weaning. It is aimed to see if GILAX cleanser could provide effective health protection on weans. 30 A randomized block design assigned sex-matched individual pens per treatment group at weaning. Each pen held 15 piglets (21 days old) three pens were used. 1) Positive control. The piglets received in-feed amoxicillin at 50mg/kg/day for 21 days.
WO 2005/020997 PCT/AU2004/001151 20 2) Negative control. The piglets received no medicated feed and water for 21 days. 3) GILAX cleanser treatment. The piglets received in feed GILAX cleanser (5mg/kg/day berberine chloride+ 50mg/kg/day 40% oxidized cellulose) for 21 5 days. Any sick piglets noted during the study were dosed with injectable amoxicillin (2g/day x 2). In this study the piglets in group 1 (Amoxicillin) and group3 (GILAX) were all healthy, no injectable amoxicillin was needed. Two piglets in 10 group 2 (negative control) were sick and these were injected with antibiotic. Footnotes [1] Tancr6de C. Role of human microflora in health and disease. Eur. J. Clin. Microbiol. Infect. Dis. 11:1012 (1992). 15 [2] Chopra, R.N., et al., Pharmacological action of berberine. Indian J. Med. Res. 19, 1193-1203 (1932). [3] Zhang, M. F. et al., Two new 8-oxotetrahydroprotoberberine alkaloids, gusanlung A and B from Acangelisia gusanlung. Planta Med. 57, 457-459 (1991). 20 [4] Tang, W. et al., In Chinese Drugs of Plant Origin. pp361-371 (1992), Springer-Verlag Press: London. [5] Dutta, N.K. et al., Berberine in toxin induced experimental cholera. Br. J. Pharm.. 44,153-159 (1972). [6] Sabir, M. et al., Antagonism of cholera toxin by berberine in the 25 gastrointestinal tract of adult rats. Indian J. Med. Res. 65, 305-313 (1977). [7] Sack, R.B. et al., Berberine inhibits intestinal secretory response of Vibrio cholerae and Escherichia coli enterotoxins. Infect. Immun. 35, 471-475 (1982). [8] Yamamoto, K. et al., Pharmacological studies on anti-diarrhoeal effects of a preparation containing berberine and gerainii herba. Nippon Yakurigaku 30 Zasshi, 101, 169-175 (1993). [9] Tai, Y.H. et al., Antisecretory effects of berberine in rat ileum. Am. J. Physiol. 241, G253-G258 (1981).
WO 2005/020997 PCT/AU2004/001151 21 [10] Guandalini, S. et al., Effects of berberine on basal and secretagogue modified ion transport in the rabbit ileum in vitro. J. Pediatr. Gastroenterol. 6, 953-960 (1987). [11] Taylor, C.T. et al., Berberine inhibition of electrogenic ion transport in rat 5 colon. Br. J. Pharmacol. 116, 2267-2672 (1995). Berberine inhibits ion transport in human colonic epithelia. Eur. J. Pharmacol .368, 111-118 (1999). [12] Chinese Pharmacopoeia Part Il p.437-439 5th Edition (1990). [13] Baird A.W et al., Non-antibiotic anti-diarrhoeal drugs: factors affect oral bioavailability of berberine and loperamide in intestinal tissue. Adv. Drug Deliv. 10 Review 23, 111-120 (1997). [14] U.S. 2,801,216. [15] U.S. 3,016,328. [16] U.S. 3,679,792. [17] U.S. 4,034,084. 15 [18] a) U.S. 6,410,040. b) PCT/AU96/00328.

Claims (56)

1. A composition including a compound of Formula (1) and a polymeric 5 dialdehyde for the treatment of gastrointestinal (GI) functional disorders or related conditions and/or for promoting weight gain in animals, where Formula (1) comprises: 3R4 5 2 R R H3CO +NR Formula (1) H3CO- ORI R1 R 7 R9 Rio , _ where R 1 , R 2 , R 7 , R", R 9 , and R 1 0 may be the same or different and are selected 10 from H, CH 3 , OH, OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl; R 3 , R 4 , R 5 , R 6 may be the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl, or R 5 and R 6 are the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl and R 3 and R 4 together are =0, or 15 R 4 and R 6 are the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl and R 3 and R 5 together form a double bond or are =0, or R 3 and R 4 , are the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl and R 5 and R t ogether are =0; 20 R" and R 12 together form =CH 2 , or R" and R 12 may be the same or different and are independently selected from the group consisting of H, CH 3 , CH 2 CH 3 and CH 2 CH 2 CH 3 ; and X is selected from the group consisting of Cl, Br, SO 4 , I and R 13 COO, where R 13 is CH 3 or poly acids. 25
2. A composition according to claim 1, wherein R 5 and R 6 are the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl and R 3 and R 4 together are =0. WO 2005/020997 PCT/AU2004/001151 23
3. A composition according to claim 1, R 4 and R 6 are the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl and R 3 and R 5 together form a double bond or are =0. 5
4. A composition according to claim 1 wherein R 3 and R 4 , are the same or different and are selected from H, CH 3 , OCH 3 , C 2 H 5 , OC 2 H 5 OCH 2 Ph, OCH 2 PhNO 2 , F or Cl and R 5 and R t ogether are =0 10 5. A composition according to any one of claims 1 to 4 wherein the GI functional disorders or related conditions include Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD), Colitis, Crohn's disease and coeliac disease. 15 6. A composition according to any one of claims 1 to 4 wherein the animals include humans and other primates, birds including poultry, ungulates including cattle, sheep, cervidae and swine, fish including crustaceans and molluscs, reptiles, rodents, canines and felines. 20 7. A composition according to any one of claims 1 to 4 wherein R', R 2 , R 3 , R 4 , R 5 , R 6 ,R 7 , R", R 9 and R 1 U are hydrogen, R" and R 12 together form =CH 2 and X is Cl so that Formula (1) is berberine chloride.
8. A composition according to any one of claims I to 4 wherein the 25 polymeric dialdehyde is poly-(2-propenal, 2-propenoic acid).
9. A composition according to any one of claims 1 to 4 wherein the polymeric dialdehyde is a dialdehyde polysaccharide. 30 10. A composition according to claim 9 wherein the dialdehyde polysaccharide comprises a compound of Formula (2). WO 2005/020997 PCT/AU2004/001151 24 Formula (2) HO - R R -Rrr Rp - R -Rr -H 1 1 1n qn n wherein each of the monomers RP and Rr, are independently selected from the group consisting of: 14 R14 R -- CH2 000 0 O H 0HJ, HOH 5H OH (A) and (B) and (C) and each monomer Rq is independently selected from the group consisting of: 14 14 R R O0O ~~~ O-- CH2 0 ~CH2 0 0 0 (D) and (E) and (F) and ~CH2 0 HO HO _0- 10 (G) wherein each R 14 may be the same or different and is independently selected from the group consisting of CH 2 OH, COOH, CH 2 0CH 2 COOH and CH 2 0R 15 , where R 15 is selected from the group consisting of CH 2 C 6 H 4 COOH, C 6 H 4 COOH 15 and CH 2 (CH 2 )yCOOH where y=1 to 20; wherein p1 - Pn may be the same or different and are each independently selected from the range 0 to n; q1 - qn may be the same or different and are each independently selected from the range 1 to m; r 1 -- rn may be the same or different and are each independently selected from the range of 0 to n, n is an 20 integer greater than 0 and m is an integer greater than 1; and wherein WO 2005/020997 PCT/AU2004/001151 25 qil+--+qn 100% 30% pI+-+pn+ql+---+qn+rl+-+rn 5 11. A composition according to claim 10 wherein each RP and Rr are (A) and Rq is (D), so that the polymeric dialdehyde is oxidised cellulose.
12. A composition according to claim 11 wherein R 14 is CH 2 OH or CH 2 0CH 2 COOH. 10
13. A composition according to claim 10 wherein each RP and Rr are (B) and Rq is (E), so that the polymeric dialdehyde is oxidised starch or dextrin.
14. A composition according to claim 10 wherein each RP and Rr are (C) and 15 Rq is (F) and/or (G) so that the polymeric dialdehyde is oxidised dextran.
15. A composition according to claim 11, 13 or 14, wherein: q _+- _+_n x 100% 2 40% p1+-+pn+q1+-+qa+r1+-+rn 20
16. A composition according to claim 15, wherein: 40%!5 qI+:-+An x 100%5 60% p1+-+pn+q1+-+qa+r1+-+rn 25
17. A composition according to claim 10 wherein the compound of Formula (2) has a molecular weight of from 1,000 to 1,000,000.
18. A composition according to claim 17 wherein the compound of Formula 30 (2) has a molecular weight of from 10,000 to 750,000.
19. A composition according to claim 10 wherein the compound of Formula (2) is water-insoluble and has a particle size of from 5p to 1 OO. 35 20. A composition according to claim 19 wherein the compound of Formula (2) has a particle size of from 5 t to 30t. WO 2005/020997 PCT/AU2004/001151 26
21. A composition according to claim 10, where the weight ratio of Formula (1) to Formula (2) ranges from 1:1 to 1:100. 5 22. A composition according to claim 21 wherein the weight ratio of Formula (1) to Formula (2) ranges from 1:10 to 1:40.
23. A composition according to claim 11 wherein Formula (1) is berberine chloride and the oxidised cellulose comprises from 30 to 100% oxidised 10 monomer.
24. A composition according to claim 23 wherein the oxidised cellulose comprises from 40 to 60% oxidised monomer. 15 25. A composition according to claim 1 for promoting weight gain in humans and other primates, birds, ungulates, fish, reptiles, rodents, canines and felines.
26. A pharmaceutical composition including a compound of Formula (1) and a polymeric dialdehyde, together with one or more further active ingredients 20 selected from vitamins, antibiotics, antiseptic agents, surfactants, antidiarrhoeal agents, anti-constipation agents, enzymes including digestive enzymes, probiotic bacteria, herbs, vaccines, ulcer healing agents, growth factors, Gibberellins and glucans. 25 27. A pharmaceutical composition including a compound of Formula (1) and a compound of Formula (2), together with one or more further active ingredients selected from other polydialdehydes, vitamins, antibiotics, antiseptic agents, surfactants, antidiarrhoeal agents, anticonstipation agents, enzymes including digestive enzymes, probiotic bacteria, herbs, vaccines, ulcer healing agents, 30 growth factors, Gibberellins and glucans.
28. A pharmaceutical composition in accordance with claim 26, wherein the composition also contains one or more pharmaceutically acceptable excipients selected from binding agents, lubricants, fillers, disintegrants, wetting agents, WO 2005/020997 PCT/AU2004/001151 27 suspending agents, viscosity enhancers, buffers and isotonicity-adjusting agents.
29. A pharmaceutical composition in accordance with claim 27, wherein the 5 composition also contains one or more pharmaceutically acceptable excipients selected from binding agents, lubricants, fillers, disintegrants, wetting agents, suspending agents, viscosity enhancers, buffers and isotonicity-adjusting agents. 10 30. A kit including a quantity of a compound of Formula (1) and a quantity of polymeric dialdehyde for the treatment of gastrointestinal (GI) functional disorders or related conditions including Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD) including colitis, Crohn's disease and coeliac disease; and for promotion of weight gain in animals including humans. 15
31. A kit in accordance with claim 30 wherein the weight ratio of the quantity of compound of Formula (1) to the quantity of polymeric dialdehyde ranges from 1:1 to 1:100. 20 32. A kit in accordance with claim 31 wherein the weight ratio of the quantity of compound of Formula (1) to the quantity of polymeric dialdehyde ranges from 1:10 to 1:40.
33. A method of treatment of gastrointestinal disorders, including 25 administering to an animal suffering from a gastrointestinal disorder an effective amount of a compound of Formula (1) in conjunction with an effective amount of polymeric dialdehyde.
34. A method according to claim 33 wherein the gastrointestinal disorders 30 include Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD), colitis, Crohn's disease and coeliac disease.
35. A method according to claim 33 wherein the compound of Formula (1) is berberine chloride. WO 2005/020997 PCT/AU2004/001151 28
36. A method according to claim 33 wherein the polymeric dialdehyde is poly-(2-propenal, 2-propenoic acid). 5 37. A method according to claim 34 wherein the polymeric dialdehyde is a dialdehyde polysaccharide.
38. A method according to claim 37 wherein the dialdehyde polysaccharide comprises a compound of Formula (2). 10
39. A method according to claim 38 wherein the compound of Formula (2) is oxidized cellulose.
40. A method according to claim 39 wherein: 15 40%5 ql+--+sg x 100%5 s60% p1+-+pn+ql+-+q+r1+-r
41. A method according to claim 27 wherein the compound of Formula (1) and the polymeric dialdehyde are administered sequentially. 20
42. A method of claim 33 in which the compound of Formula (1) and the polymeric dialdehyde are administered simultaneously.
43. A method of claim 42 wherein the compound of Formula (1) and the 25 polymeric dialdehyde are administered in the form of a composition containing both the compound of Formula (1) and the polymeric dialdehyde.
44. A method of promoting weight gain in animals including humans, including administering to an animal, including a human, effective amounts of a 30 compound of Formula (1) and polymeric dialdehyde in conjunction.
45. A method according to claim 44 wherein the compound of Formula (1) is berberine chloride. WO 2005/020997 PCT/AU2004/001151 29
46. A method according to claim 44 wherein the polymeric dialdehyde is poly-(2-propenal 2-propenoic acid).
47. A method according to claim 44 wherein the polymeric dialdehyde is a 5 dialdehyde polysaccharide.
48. A method according to claim 47 wherein the dialdehyde polysaccharide comprises a compound of Formula (2). 10 49. A method according to claim 48 wherein the compound of Formula (2) is oxidized cellulose.
50. A method according to claim 49 wherein: 40%5 sq+-_+_ _ x 100%5 60% 15 p1+-+pn+q1+-+qn+r1+-rn
51. A method according to claim 44 wherein the compound of Formula (1) and the polymeric dialdehyde are administered sequentially. 20 52. A method of claim 44 in which the compound of Formula (1) and the polymeric dialdehyde are administered simultaneously.
53. A method of claim 52 wherein the compound of Formula (1) and the polymeric dialdehyde are administered in the form of a composition containing 25 both the compound of Formula (1) and the polymeric dialdehyde.
54. Use of a compound of Formula (1) and a polymeric dialdehyde in the preparation of a composition for the treatment of gastrointestinal (GI) functional disorders or related conditions, or for promoting weight gain in animals including 30 humans.
55. Use of a compound of Formula (1) and a polymeric dialdehyde in accordance with claim 54 wherein the GI functional disorders or related conditions include Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease 35 (IBD), colitis, Crohn's disease and coeliac disease WO 2005/020997 PCT/AU2004/001151 30
56. Use of a compound of Formula (1) and a polymeric dialdehyde in accordance with claim 54 wherein the compound of Formula (1) is berberine chloride. 5
57. Use of a compound of Formula (1) and a polymeric dialdehyde in accordance with claim 54 wherein the polymeric dialdehyde is poly-(2-propenal 2-propenoic acid). 10 58. Use of a compound of Formula (1) and a polymeric dialdehyde in accordance with claim 54 wherein the polymeric dialdehyde is a dialdehyde polysaccharide.
59. Use of a compound of Formula (1) and a polymeric dialdehyde in 15 accordance with claim 58 wherein the dialdehyde polysaccharide comprises a compound of Formula (2).
60. Use of a compound of Formula (1) and a polymeric dialdehyde in accordance with claim 59 wherein the compound of Formula (2) is oxidised 20 cellulose.
61. Use of a compound of Formula (1) and a polymeric dialdehyde in accordance with claim 60 wherein: 40%:5 gq_---+qn x 100%5 s60% 25 p1+-+pn+q1+---+qa+r1+---rn
62. A method according to claim 44 wherein the compound of Formula (1) and the compound of Formula (2) are used as feed additives. 30 63. A modified food containing 0.1 to 50 ppm of a compound of Formula (1) and from 1 to 400 ppm of a polymeric dialdehyde.
64. A modified food in accordance with claim 63 wherein the food contains from 2 to 10 ppm of a compound of Formula (1) and from 10 to 200 ppm of a 35 compound of Formula (2). WO 2005/020997 PCT/AU2004/001151 31 6. A modified food according to claim 63 wherein Formula (1) is berberine chloride and wherein the polymeric dialdehyde is oxidized cellulose. 5 66. A method of treatment for Irritable Bowel Syndrome, Inflammatory Bowel Disorder, Colitis, Crohn's disease and coeliac disease including administering an effective amount of a compound of Formula (1) to an animal in need thereof.
67. A method of treatment for Irritable Bowel Syndrome, Inflammatory Bowel 10 Disorder, Colitis, Crohn's disease and coeliac disease including administering an effective amount of a compound of Formula (2) to an animal in need thereof.
68. A method of promoting weight gain in animals including administering an effective amount of a compound of Formula (1). 15
69. A method of promoting weight gain in animals including administering an effective amount of a compound of Formula (2).
70. Use of a compound of Formula (1) in the preparation of a composition for 20 the treatment of gastrointestinal (GI) functional disorders or related conditions, or for promoting weight gain in animals.
71. Use of a compound of Formula (2) in the preparation of a medicament for the treatment of gastrointestinal (GI) functional disorders or related conditions, 25 or for promoting weight gain in animals.
72. Use of a compound in accordance with either one of claims 70 or 71, wherein the GI functional disorders or related conditions include Irritable Bowel Syndrome (IBS); Inflammatory Bowel Disease (IBD) including colitis, Crohn's 30 disease and coeliac disease
73. A composition containing berberine chloride and 40% oxidised cellulose substantially as hereinbefore described with reference to example 5, 6 or 10. WO 2005/020997 PCT/AU2004/001151 32
74. A method of treatment substantially as herein before particularly described with reference to examples 4, 7, 8 or 10.
AU2004267873A 2003-08-28 2004-08-26 Compositions for veterinary and medical applications Expired AU2004267873C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2004267873A AU2004267873C1 (en) 2003-08-28 2004-08-26 Compositions for veterinary and medical applications

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
AU2003904654A AU2003904654A0 (en) 2003-08-28 Compositions for veterinary and medical applications
AU2003904654 2003-08-28
AU2003904817 2003-09-04
AU2003904817A AU2003904817A0 (en) 2003-09-04 Compositions for veterinary and medical applications
PCT/AU2004/001151 WO2005020997A1 (en) 2003-08-28 2004-08-26 Compositions for veterinary and medical applications
AU2004267873A AU2004267873C1 (en) 2003-08-28 2004-08-26 Compositions for veterinary and medical applications

Publications (3)

Publication Number Publication Date
AU2004267873A1 true AU2004267873A1 (en) 2005-03-10
AU2004267873B2 AU2004267873B2 (en) 2008-02-28
AU2004267873C1 AU2004267873C1 (en) 2008-12-11

Family

ID=36084063

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004267873A Expired AU2004267873C1 (en) 2003-08-28 2004-08-26 Compositions for veterinary and medical applications

Country Status (1)

Country Link
AU (1) AU2004267873C1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3398448A1 (en) * 2017-05-05 2018-11-07 Vall Garraf, S.L. Fattening additive for animal nutritional composition

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8927565B2 (en) 2003-08-28 2015-01-06 Australian Biomedical Company Pty. Ltd. Compositions for veterinary and medical applications

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0633259B2 (en) * 1985-07-09 1994-05-02 生体機能利用化学品新製造技術研究組合 Method for separating and purifying berberine alkaloids
US5409903A (en) * 1992-02-18 1995-04-25 Urecap Corporation Method and compositions for the treatment of H. pylori and dermatitis

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3398448A1 (en) * 2017-05-05 2018-11-07 Vall Garraf, S.L. Fattening additive for animal nutritional composition

Also Published As

Publication number Publication date
AU2004267873C1 (en) 2008-12-11
AU2004267873B2 (en) 2008-02-28

Similar Documents

Publication Publication Date Title
CN101428057B (en) Antimicrobial powder for reinforcing immune, strengthening the body resistance and removing toxicity for livestock and poultry
US9730971B2 (en) Feed for preventing and/or treating diseases caused by clostridium bacterium in livestock, and agent against clostridium
CN110692863A (en) Plant extract feed additive for improving liver and intestine protecting capability of tilapia
Aluko et al. Combined effects of chitosan and microencapsulated Enterococcus faecalis CG1. 0007 probiotic supplementation on performance and diarrhea incidences in enterotoxigenic Escherichia coli K88+ challenged piglets
CN114711336A (en) Anti-diarrhea formulations avoiding antimicrobial resistance
Hassan Antimicrobial activities of saponin-rich guar meal extract
CA2536489C (en) Compositions of isoquinolines and polymeric dialdehydes for veterinary and medical applications
US20160030475A1 (en) Use of a clay product or a clay blend product to decrease the effects of bacterial disease in shrimp
CN102973690A (en) Chinese herbal medicine preparation for treating pig gastroenteritis
AU2004267873C1 (en) Compositions for veterinary and medical applications
EP0820306B1 (en) Pharmaceutical composition containing pectin and a phospholipid, used as an antidiarrheal and antiulcer agent
ES2369138T3 (en) COMPOSITIONS FOR VETERINARY AND MEDICAL APPLICATIONS.
CN104784299B (en) A kind of fermented tcm composition of kuh-seng containing wolf&#39;s milk and radix scutellariae and preparation method thereof
KR20010068226A (en) composition containing chitosan oligo saccharide and aminoethyl sulfonic acid for use as fodder and drinking water additives
KR100371090B1 (en) The composition of antibacterial complex for animal
CN102160602B (en) Natural compound feed additive for reducing cholesterol in egg and preparation method thereof
KR100371092B1 (en) The composition of antibacterial complex for animal
CN104688883B (en) A kind of Chinese medicine composition of kuh-seng containing wolf&#39;s milk and radix scutellariae and preparation method thereof
RU2415684C2 (en) Pharmaceutical composition for treatment and/or prevention of intestinal disbiosis in process of antibacterial therapy in warm-blooded animals: cattle, pigs, domestic animals, in particular dogs, cats and poultry and method of treatment and/or prevention of intestinal disbiosis in process of antibacterial therapy in warm-blooded animals: cattle, pigs, domestic animals, in particular dogs, cats and poultry
Fairchild et al. Effect of hen age, Bio-Mos and Flavomycin on susceptibility of turkey poults to oral Escherichia coli challenge.
CN114129689B (en) Traditional Chinese medicine composition, feed additive and new application thereof
CN112716942B (en) Indole and application of duck feed containing indole in maintaining duck intestinal health and/or preventing necrotic enteritis of ducks
CN107801854A (en) A kind of agent of feed for nursing sow additive for being used to prevent the white scour of piglet
Dong et al. Evaluation of the efficacy of the protopine total alkaloids of Macleaya cordata (Willd.) R. Br. in controlling E. coli infection in broiler chickens
JP2599162B2 (en) Prevention and treatment of Salmonella enteritidis infection in livestock and poultry

Legal Events

Date Code Title Description
DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 15 FEB 2008.

FGA Letters patent sealed or granted (standard patent)
DA2 Applications for amendment section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 15 FEB 2008.

TH Corrigenda

Free format text: IN VOL 22, NO 10, PAGE(S) 1081 UNDER THE HEADING AMENDMENTS -APPLICATION FOR AMENDMENTS DELETE ALL REFERENCE TO 2004267873

DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS AS SHOWN IN THE STATEMENT(S) FILED 15 FEB 2008

PC Assignment registered

Owner name: IRICCORGPHARM PTY LTD

Free format text: FORMER OWNER(S): AUSTRALIAN BIOMEDICAL COMPANY PTY LTD

HB Alteration of name in register

Owner name: IRP HEALTH PTY LTD

Free format text: FORMER NAME(S): IRICCORGPHARM PTY LTD

MK14 Patent ceased section 143(a) (annual fees not paid) or expired