AU2004203417A1 - Virulence genes, proteins, and their use - Google Patents

Description

Regulation 3.2

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT

APPLICANT:

Invention Title: Microscience Limited VIRULENCE GENES, PROTEINS, AND THEIR USE The following statement is a full description of this invention, including the best method of performing it known to me: VIRULENCE GENES, PROTEINS, AND THEIR USE Field of the Invention This Invention relates to virulence genes and proteins, and their use. More particularly, it relates to genes and proteins/peptides obtained from Neisserfa rneningtidis, and their use in therapy and in screening for drugs.

Backaround of the Invention Neisserfa meningltidis is a Gram-negative bacterial pathogen that is implicated in septic shock and bacterial meningitis. This bacterium is a leading cause of bacterial meningitis in developed countries, and causes large-scale epidemics in Africa and China. In the UK, Nelsseda meningitidis is the leading cause of death in childhood apart from road traffic accidents. The bacterium naturally inhabits the human nasopharynx and then gains access to the blood stream from where it causes severe septicaemla or meningitis. Although current anti-microblals are effective in eliminating the bacterium from the body, the mortalilty from menigococcal septicaemia remains substantial. It would be desirable to provide means for treating or preventing conditions caused by Nelssera menlngtldis, e.g. by immunisation.

Summary of the Invention The present invention is based on the discovery of virulence genes in Nelssera menlngltidis.

According to a first aspect of the invention, a peptide of the invention is encoded by an operon including any of the nucleotide sequences identified in claim 1, or a homologue thereof in a Gram-negative bacterium, or a functional fragment thereof, for therapeutic or diagnostic use.

The peptides may have many therapeutic uses for treating Neisseria infections, including use In vaccines for prophylactic application.

According to a second aspect, a polynucleotlde encoding a peptlde defined above, may also be useful for therapy or diagnosis.

According to a third aspect, the genes that encode the peptides may be utilised to prepare attenuated microorganisms. The attenuated microorganisms will usually have a mutation that disrupts the expression of one or more of the genes identified herein, to .provide a strain that lacks virulence. These microorganisms will also have use in therapy and diagnosis.

According to a fourth aspect, the peptides, genes and attenuated microorganisms according to the Invention may be used in the treatment or prevention of a condition associated with infectioA by Nelsseria or Gram-negative bacteria.

Description of the Invention The present invention is based on the discovery of genes encoding peptides which are implicated in virulence. The peptides and genes of the invention are therefore useful for the preparation of therapeutic agents to treat Infection. It should be understood that references to therapy also Include preventative treatments, e.g.

vaccination. Furthermore, while the products of the Invention are intended primarily for treatment of Infections in human patients, veterinary applications are also considered to be within the scope of the Invention.

The present Invention is described with reference to Neisseria meningitidis.

However, all the Neisseria strains, and many other Gram-negative bacterial strains are likely to Include related peptides or proteins having amino add sequence identity or similarity to those Identified herein. Organisms likely to contain the peptides include, but are not limited to the genera Salmonella, Enterobacter, Klebsiella, Shigella and Yerslnla.

The experiments carried out to identify the virulence genes of the invention utilised N. meningitidis strain B. Homology searches were performed on the strain A database, however the proteins and genes from strain B are preferred.

Preferably, the peptides that may be useful in the various aspects of the invention have greater than a 40% similarity with the peptides identified herein. More preferably, the peptides have greater than 60% sequence similarity. Most preferably, the peptides have greater than 80% sequence similarity, e.g. 95% similarity. With regard to the polynudeotide sequences Identified herein, related polynucleotides that may be useful in the various aspects of the invention may have greater than Identity with the sequences identified herein. More preferably, the polynudeotide sequences have greater than 60% sequence identity. Most preferably, the polynucleotide sequences have greater than 80% sequence identity, e.g. 95% identity.

The terms "similarity" and "Identity" are known in the art. The use of the term "Identity" refers to a sequence comparison based on Identical matches between correspondingly identical positions in the sequences being compared. The term "similarity" refers to a comparison between amino acid sequences, and takes Into account not only Identical amino acids In corresponding positions, but also functionally similar amino adds in corresponding positions. Thus similarity between polypeptide sequences indicates functional similarity, In addition to sequence similarity.

Levels of Identity between gene sequences and levels of Identity or similarity between amino acid sequences can be calculated using known methods. In relation to the present invention, publicly available computer based methods for determining identity and similarity include the BLASTP, BLASTN and FASTA (Atschul et at, J.

Molec. Biol., 1990; 215:403-410), the BLASTX program available from NCBI, and the Gap program from Genetics Computer Group, Madison WI. The levels of similarity and identity provided herein, were obtained using the Gap program, with a Gap penalty of 12 and a Gap length penalty of 4 for determining the amino acid sequence comparisons, and a Gap penalty of 50 and a Gap length penalty of 3 for the polynucleotlde sequence comparisons.

Having characterised a gene according to the invention, it is possible to use the gene sequence to search for related genes or peptides in other microorganisms. This may be carried out by searching in existing databases, e.g. EMBL or GenBank.

Peptides or proteins according to the invention may be purified and isolated by methods known in the art. In particular, having Identified the gene sequence, it will be possible to use recombinant techniques to express the genes In a suitable host. Active fragments and related moleculescan be Identified and may be useful in therapy. For examplethe peptides or their active fragments may be used as antigenic determinants in a vaccine, to elicit an immune response. They may also be used in the preparation of antibodies, for passive immunisation, or diagnostic applications. Suitable antibodies include monoclonal antibodies, or fragments thereof, including single chain Fv fragments. Methods for the preparation of antibodies will be apparent to those skilled in the art.

Active fragments of the peptides are those that retain the biological function of the peptide. For example, when used to elicit an Immune response, the fragment will be of sufficient size, such that antibodies generated from the fragment will discriminate between that peptide and other peptides on the bacterial microorganism. Typically, the fragment will be at least 30 nucleotides (10 amino acids) in size, preferably nucleotides (20 amino acids) and most preferably greater than 90 nucleotides amino acids) in size.

It should also be understood, that in addition to related molecules from other microorganisms, the invention encompasses modifications made to the peptides and polynucleotides Identified herein which do not significantly alter the biological function.

It will be apparent to the skilled person that the degeneracy of the genetic code can result in polynudeotides with minor base changes from those specified herein, but which nevertheless encode the same peptides. Complementary polynucleotildes are also within the Invention. Conservative replacements at the amino acid level are also envisaged, i.e. different acidic or basic amino acids may be substituted without substantial loss of function.

The preparation of vaccines based on attenuated microorganisms is known to those skilled in the art. Vaccine compositions can be formulated with suitable carriers or adjuvants, e.g. alum, as necessary or desired, to provide effective Immunisation against infection. The preparation of vaccine formulations will be apparent to the skilled person. The attenuated microorganisms may be prepared with a mutation that disrupts the expression of any of the genes identified herein. The skilled person will be aware of methods for disrupting expression of particular genes. Techniques that may be used include insertional inactivation or gene deletion techniques. Attenuated microorganisms according to the invention may also comprise additional mutations in other genes, for example in a second gene identified herein or in a separate gene required for growth of the microorganism, e.g. an aro mutation or, with regard to Salmonella, in a gene located in the SPI2 region identified in WO-A-96/17951.

Attenuated microorganisms may also be used as carrier systems for the delivery of heterologous antigens, therapeutic proteins or nucleic acids (DNA or RNA). In this embodiment, the attenuated microorganisms are used to deliver a heterologous antigen, protein or nucleic acid to a particular site in vivo. Introduction of a heterologous antigen, peptide or nucleic acid into an attenuated microorganism can be carried out by conventional techniques, Including the use of recombinant constructs, e.g. vectors, which comprise polynucleotides that express the heterologous antigen or therapeutic protein, and also include suitable promoter sequences. Alternatively, the gene that encodes the heterologous antigen or protein may be incorporated into the genome of the organism and the endogenous promoters used to control expression.

More generally, and as is well known to those skilled in the art, a suitable amount of an active component of the invention can be selected, for therapeutic use, as can suitable carriers or exciplents, and routes of administration. These factors would be chosen or determined according to known criteria such as the nature/severity of the condition to be treated, the type and/or health of the subject etc.

In a separate embodiment, the products of the invention may be used In screening assays for the Identification of potential antimicrobial drugs or for the detection for virulence. Routine screening assays are known to those skilled in the art, and can be adapted using the products of the invention In the appropriate way. For example, the products of the invention may be used as the target for a potential drug, with the ability of the drug to inactivate or bind to the target indicating its potential antimicrobial activity.

The various products of the invention may also be used in veterinary applications.

The following is a brief overview of the experimental procedure used to identify the virulence genes.

Signature-tagged mutagenesis (STM) (Hensel et al., Science, 1995; 269: 400- 403) was used to identify genes in N. meningitdls that are essential for septicemic infection. In STM, individual mutants are tagged with unique sequence Identifiers, allowing large numbers of mutants to be analyzed simultaneously. However, it Is necessary to construct libraries of Insertlonal mutants, so far a limitation in studying N.

meningitidis. Mutagenesis was accomplished successfully using a method In which Neissera DNA is modified in vitro using purified components of Tn 10 transposition. As N. meningitidis efficiently takes up exogenous DNA, the modified alleles are then introduced into N. meningitidis by transformation. The mutants can then be screened for their ability to cause systemic infection.

The vector pSTM 115 (Sun et al. Nature Medicine, 2000; 6(11): 1269-1273) was used as the transposon donor for in vitro mutagenesis. 96 pSTM115 derivatives, each containing unique signature tags, were included in 96 separate transposition reactions.

The modified genomic DNA was repaired, and returned to the host by transformation.

To determine whether TnlO insertion occurs at diverse sites, 40 transformants were assessed from a single transposition reaction by Southern blot analysis. Each had a single, distinct Tn 10 Insertion. To establish whetherTn 10 integration was stable during systemic infection of infant rats, the hydrldization patterns of six mutants before and after passage through rats were compared. Identical hybridization patterns before and after infection were obtained.

The experimental conditions used were as follows: Bacterial strains and growth: C311+ is an ET-5, serogroup B N. meningitidis isolate from a patient with invasive meningococcal infection (Virji etal., Mol. Microblol., 1991; 5: 1831-1841). N.

meningitidiswas grown on brain-heart infusion medium with 5% Levinthal's supplement E. coli strains were propagated on Luria Bertani media. Kanamycin was added to solid media as required at concentrations of 75 and 50 pg/ml for N. meningitidis and E. coll, respectively.

In vitro transposition and insertion site characterization: The pACYC184 origin of replication in pSTM115 was used to Isolate the insertion site by marker rescue. Nucleotide sequencing was carried out using the dyetermination method (Perkin Elmer, Norwalk, Connecticut) with primers NG62 TTGGTAATTGGTTGTAACACTGG-3) (SEQ ID NO. 209) or NG99 ATTCTCATGTTTGACAGCG-3') (SEQ ID NO. 210). Homology searches were performed against protein databases (http://www.ncbi.nlm.nih.gov/), including the serogroup A and B N.meningitldis and the N. gonorrhoeae genome sequences (http:/www.sanger.ac.uk/Projects/Nmjeningitidis and http://www.tigr.org, and http://dnal.chem.ou.edu/gono.html, respectively).

Tag amplification, cloning and Southern blot analyses: Hybridizations and preparations of dot blots were performed as described in Hensel et at,, supra, except that tags were amplified with primers NG13 ATCCTACAACCTCAAGCT-3') (SEQ ID NO. 211) and NG14 ATCCCATTCTAACCAAGC-3') (SEQ ID NO. 212), and PCR products, rather than plasmid DNA, were fixed onto membranes. Oligonucleotides S1 AAGAGATTACGCGCAGACC-3') (SEQ ID NO. 213) and S2 AATACGCAACCGCCTCTC-3') (SEQ ID NO. 214) anneal to sequences in pSTM115 flanking the 'signature tags' and were used to amplify a 367-base-pair product from each pSTM115 derivative. For Southern blot analysis, the kanamycin-resistance cassette from pSTM 115 was labelled using the random primers method (NEB), and was used as a probe against genomic DNA digested with Clal.

Animal model: For screening the STM pools, mutants were grown individually for 18 h in microtiter plates. The bacteria were pooled, then re-suspended in PBS. Wistar rats days old) were inoculated intraperitoneally with 100 pl of the suspension, and were monitored for 48 h. To establish the competitive index of a mutant, wild-type and mutant bacteria were grown for 18 h on solid media and collected into PBS, and rats were inoculated with a 1:1 ratio of mutant to wild-type cells In a total inoculum of 5 x CFU. The proportion of mutant (kanamycin-resistant) to wild-type (kanamycin-sensitive) bacteria was determined by plating replicate samples to media with or without added antibiotic.

The results of the homology searches are shown in Table 1.

IsWe.1 SEQ ID NO. PROTEIN ACCESSION NO. ORGANISM s3&4 3'debydroqulnate synthase NMB0647 N. gonoihoese 26 Glycosyl transferase LS12 N. gonorrheae so Polyrbonurleotlde nucleotlyl NMB0758 N. menlngitidis transferase 37 38 Phosphoribosyl formyt giyclnamlde PURL E. coil synthase 43 &£44 Shikimate dehydrogenase AROE N. menlngitidis 47 40 Hypothetical 21.7 lcD protein NMBOS73 N. menlngitidis 53 54 Putative cell binding factor NMB0345 N menlngitidls 57 58 Hypothetical protein H10833 H. infiuenzae 61 &602 Na+/H+ entiporter NMSN053O N. merningitlis 83 64 Chorisminte synthase AROC V. angullarum 67 68 Paraquat-inducibie protein B PQ15B E. coil 71 £72 5-methyltetrahydrapteoyyl NMB0944 N. meningitis trlutamate-homocystelne methyl transferase 79 80 0-1,2 N-aoetylglucosarnine RFAK N. meflirgifidis trnnsferese 83 84 Putative RNA methyisse NM81 348 N. menngltis 89 90 1-isolate perrnsase NMB0S43 N. menlngltldis 91 92 ABC transporter NMB1240 N. meningitis 103 104 Probable GTP-binding protein H10393 H. influenza.

1094& 110 Capsule polysacchsridle rnodflcation UPS N. meningitidis protein 113& 114 Hypothetical 17.8 kD) protein NMB0734 N. meningitidis 115 &116 E. coil hypothetical protein YIGC 8. typhirowurri 119 120 Ribonuolease Ill NMB0688 N. meningitidila 121 122 AMPD protein NMBOOO8 N. meningitidis 123 124 5-rn thyltetrahydropteroylyi NMB0944 N. menlngitldis tllglutamate-homocystelne methyl __________trnnsferase 133 134 Putative ATP-depenent RNA NMBI422 N. meningitidis helicase 137 138 Putative RNA methylase NM51348 N. meningitidis 141 142 Shikimate dehydrogenase AROE N. meningitids 143 144 Putative outer membrane protein OMPI N. menlngitdls 145 148 TONS protein TONB N. meningitidis 147 148 Putative apolipoprotein N-acyl NMBD713 N. meningitidis transferase 149 150 Transposase NMBO991 N. meningitidls 153 154 UTP-glucose-lrphosphate NMBOOSO N. meningitidis urdylyitransferase 157 158 ADP Heptose-LPS heptosyl NMB1527 N. meningitidis transferase II 161 162 Putative membrane-bound lytic NMB1279 N. meningitidis murein transglycosylase B 171 172 Putative cell-binding factor NMB0345 N. meningitidis 173 174 P-amino benzoate synthetase PABB H. pylor J99 177 178 5'-methyltetrahydroplemroylyl NMB0944 N. meningitids triglutamate-homocysteine methyl transferase 183 184 Conserved hypothetical protein NMB0183 N. meningitidis 185 186 E. coil hypothetical protein YIGC 8. typhi LT2 For the remaining sequences Identified herein, no homology results were obtained.

The gene products were used to produce polydclonal antibodies that were tested in an Ellsa assay against various N. meningldis strains to evaluate their effectiveness as a vaccine candidate. The strains used were: Nelsserie meningitidis Type 1000 Nelsseia meningitidis Type NGE31 Nesseda menlnglildis Type Neissefa menlngifldis Type SW2 107 Neisserfa meningidlis Type NHG38 Nelsseria menlngftldls Type NGE28 Nelssera meningliidis Type 2996 This provides information as to the variety of N. meningitldis strains that are recognised by these antibodies.

N. Meningitidis was grown on Columbia agar with chocolated horse blood (Oxoid) for 14 hours at 37C In 5% CO 2 The cells were scraped from agar plates and resuspended In 20ml PBS in a 50ml tube. The cell suspension was heated for minutes at 56°C to kill the bacteria.

A 50 pl sample of the heat-killed N. Meningitidis was spread on the Columbia agar with the chocolated horse blood and incubated for 18 hours at 37C, 5%C0 2 This allows confirmation that all N. Meningitidis cells have been killed. The ODM of the suspension is adjusted to 0.1 OD units versus PBS.

Elisa with heat killed N. meningfldis Elisa assays were carried out using the heat-killed N. meningitidis using the following protocol. Elisa plates were coated overnight with heat-killed cells (50pl of killed bacteria in PBS to each well of 96 well plate and incubated 4"C).

Standard Elisa protocols were followed, with all incubations at 37°C for 1 hour.

PBS/3% BSA blocking solution, PBS/Tween 0.1% wash solution, anti-rabbit AP conjugate secondary antibody (Sigma) and Sigma Fast P Nitrophenyl phosphate detection reagent (Sigma) were utilised. The data was read at 405nm using an appropriate micro-titre plate reader. The sera used was that available seven days after the first booster vaccination (day 35 after first vaccination).

The antibodies tested were those raised against the gene products identified as SEQ ID NOS. 8,102,140, 158 and 202. In each case, the results showed that the antisera recognised several different strains of N. meningltidis B.

Ex vivo/ln vito screening.

Protection against meningococcal disease in humans has been associated with the presence of bacterlocidal antibodies against N. meningitidis (Goldschelder et l. J, Exp. Med., 1969; 129: 1307-1326). There Is also evidence to suggest a correlation between the presence of detectable bacterlocidal activity and protection in an In vivo model (reference Martin, J. Bacteriol., 2000; 83: 27-31). Therefore, the antiserum generated was used to evaluate the bacterlocidal activity of the antibodies generated.

The bactericidal assays were performed with pre-immune sera and the corresponding rabbit antiserum raised against the candidate antigens. Commercially available rabbit serum was used as the complement source following pre-screening to eliminate complement only killing. Dulbecco's PBS (Gibco) was used as a bufferwhere necessary. The N. meningtidls strain MC58, was grown at 37°C CO) for 14 hours prior to use in the assay.

200-400 CPU of MC58 In a 50pl volume were incubated in the presence of complement (50pl) with 100pl of serial dilutions of heat-inactivated serum. Samples at time zero were plated to Columbia agar with chocolated horse blood (Oxoid). After incubation for 60 minutes, the number of surviving bacteria was evaluated by plating to Columbia agar with chocolated horse blood (Oxold). The bacteriocidal activity was expressed in terms of percentage of bacteria surviving after 60 minutes. All samples were tested in duplicate and plated in triplicate. All appropriate positive and negative controls were utilised. In each test sample, the bacteriocidal activity was substantially greater than that for the pre-immune sera.

In vivo screening.

To evaluate the protective efficacy of vaccine candidates, adult mice were immunised with the recombinant proteins identified herein as SEQ ID NOS. 102 and 108 and the protective response determined by live bacterial challenge. For each vaccine candidate 15 six week old mice (6 week old balb/C mice) were vaccinated (subcutaneously) with 25pg of antigen on two separate occasions at three week Intervals.

One week afterthe end of the Immunisation schedule, the group was challenged with the homologous bacterial strain MC58. The bacteria were inoculated intraperiponeally in a volume of 500pl in Brain Heart Infusion/ 0.5% iron dextran media at a dose of 107 cfu. Previous results have shown that iron is required for initiation of bacteraemic disease in these animals. This model has previously been used to demonstrate the protective efficacy of vaccination (Lissolo etal., Infect Immujn., 1995; 63: 884-890).

Control groups included animals vaccinated with adjuvant alone (negative control) or with adjuvant combined with purified PorA (positive control). PorA Is an outer membrane protein expressed exclusively by N. meningitidis and Is the principal target for bactericidal antibodies Induced by outer membrane vesicle vaccines. Monoclonal antibodies against PorA have also been shown to passively protect animals in the infant rat model. PorA however varies considerably between strains and so while it elicits some protection when challenged with a homologous strain, it is not an ideal vaccine candidate. Survival was monitored following challenge. The negative control showed no survival after48 hours. Those vaccinated with PorA showed 6 survivors at 72 hours.

11 Those vaccinated with the proteins of SEQ ID NOS. 102 and 108 showed 5 and 3 survivors, respectively.

Claims (17)

1. 2. A peptide according to claim 1, wherein the sequence similarity is at least
2. 3. A peptide according to claim 1, wherein the sequence similarity is at least
3. 4. A peptide according to claim 1, wherein the functional fragment is a least contiguous amino acids. A peptide according to claim 1, comprising the amino acid sequence identified herein as SEQ ID NOS. 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 38, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 92, 94, 96, 98, 100, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 142, 144, 146, 148, 150, 152, 154, 156, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206 and 208.
4. 6. A polynucleotide encoding a peptide according to any preceding claim, when used in therapy or diagnosis.
5. 7. A host transformed to express a peptide according to any of claims 1 to
6. 8. An attenuated microorganism comprising a mutation that disrupts the expression of the nucleotide sequence identified herein as SEQ ID NO. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25. 27, 29, 31, 33, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, and 207.
7. 9. A microorganism according to claim 8, wherein the mutation is insertional inactivation or a gene deletion. A microorganism according to claim 8 or claim 9, wherein the microorganism is Neisseria meningitidis.
8. 11. A microorganism according to any of claims 8 to 10, comprising a second mutation in a second nucleotide sequence.
9. 12. A microorganism according to any of claims 8 to 11, for therapeutic or diagnostic use.
10. 13. A microorganism according to any of claims 8 to 12, comprising a heterologous antigen, therapeutic peptide or nucleic acid.
11. 14. A vaccine comprising a peptide according to any of claims 1 to A vaccine comprising a microorganism according to any of claims 8 to 13.
12. 16. An antibody raised against a peptide according to any of claims 1 to
13. 17. Use of a peptide according to any of claims 1-5, a polynucleotide according to claim 6, or a microorganism according to any of claims 8-13, for the manufacture of a medicament for use in the treatment or prevention of a condition associated with infection by Neisseria or Gram-negative bacteria.
14. 18. Use according to claim 17, wherein the condition is meningitis.
15. 19. Use according to claim 17 or claim 18 for veterinary treatment. Use of: an isolated peptide comprising any of the amino acid sequences identified herein as SEQ ID NOS. 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 92, 94, 96, 98, 100, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 142, 144, 146, 148, 150, 152, 154, 156, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 20)2, 204, 206 and 208 or a related molecule having at least 60% sequence similarity at the peptide level, or a functional fragment thereof, wherein the functional fragment is capable of eliciting an immune response that discriminates between the peptide of the invention and other bacterial peptides, or an attenuated microorganism comprising a mutation that disrupts the expression of the nucleotide sequences identified herein as SEQ ID NO. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 37, 39, 41, 43, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183, 185, 187, 189, 191, 193, 195, 197, 199, 201, 203, 205, and 207 in a screening assay for the identification of an antimicrobial drug. WO 01/85772 PCT/GB01/02003 SEQUENCE LISTING <110> Microscience Limited <120> Virulence Gene and Protein, and Their Use <130> REP06436WO <140> not yet known <141> 2001-05-08 <150> 0011108.8 <151> 2000-05-08 <160> 214 <170> PatentIn Ver. 2.1 <210> 1 <211> 1674 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1674) <400> 1 atg ctg acc tat acc cog ccc gat gcc Met Leu Thr Tyr Thr Pro Pro Asp Ala ccg ccc gcc aaa ace cac Pro Pro'Ala Lys Thr His ttt gcc tgg ttg tgg ccc Phe Ala Trp Leu Trp Pro gaa aag ccg Glu Lys Pro tgg ctg Trp Leu ttg ctg ttg atg gcg Leu L LeLeu Met Ala 25 ggc gtg ttt tcc cac gat ttg tgg aat Gly Val Phe Ser His acc gcc gtc gaa gca Thr Ala Val Glu Ala Asp Leu Trp Asn 40 cct gac gaa cct gcc gtc tat Pro Asp Glu Pro Ala Val Tyr ctg gca ggc agc ccc acc cct ttg gtt gcc cat Leu Ala Gly Ser Pro Thr Pro Leu Val Ala His ctg ttc ggt caa ate gat ttc ggc ata ccg ccc gtg tat ctt tgg gtt Leu Phe Gly Gin Ile Asp Phe Gly Ile Pro Pro Val Tyr Leu Trp Val 70 75 WO 01/85772 PCT/GB01/02003 gac gcc gcg tte aaa cat ttg ctg tcg ccg tgg gct gac gac ccg tat Ala Ala Ala Phe Lys His Leu Leu Set Pro Trp Ala Ala Asp Pro Tyr 90 288 gat gcc gca cgc ttt Asp Ala Ala Arg Phe 100 tcc tgc ggc ttt gac Ser Cys Gly Phe Ala 115 gcc ggc gtg ttt ttc gcc gtt gtc gga ctg act Ala Gly Val Phe Phe Ala Val Val Gly Leu Thr ggt ttc aac Gly Phe Asn 120 ttt ttg ggc aga Phe Leu Gly Arg cac ggg cge His Gly Arg age gtc Ser Val 130 cac ttt His Phe 145 gtc ctg att ctc at ggec tgt ate ggg Val Leu Ile Leu Ile Gly Cys Ile Gly att ccg acc gta Ile Pro Thr Val ctc aac cooc got gcc gcc gcc ttt gcc gcc gcc gga Leu Asn Pro Ala Ala Ala Ala Phe Ala Ala Ala Gly ctg gtg Leu Val 160 tot ttt Ser Phe 175 ctg cac ggt tat Leu His Gly Tyr ttg gct cgc cgg Leu Ala Arg Arg cag gtg Arg Val 170 att gac goc Ile Ala Ala ctg ctc ggt Leu Leu Gly ggt tgg acg ctg atg tcg ttg gca gca get tat ccg Gly Trp Thr Leu Met Sex Leu Ala Ala Ala Tyr Pro 185 on gcg gca ttt Ala Ala Phe 195 cog tgg caa Pro Trp Gin 210 gce ctg atg ctg Ala Leu Met Leu ctg ccc gtg ctg atg ttt ttc cgt Leu Pro Val Leu Met Phe Phe Arg 205 ttg acg gca gtc gc tcg att gac Leu Thr Ala Val Ala Set Leu Ala 220 agc agg cgt ttg atg Ser Arg Arg Leu Met 215 624 672 720 768 gcc ctg ccg ctt Ala Leu Pro Leu acc gtt tac ccg Thr Val Tyr Pro etc ttg gca aaa Leu Leu Ala Lys cag cce gcg ctg Gln Pro Ala Leu gcg caa tgg ctc Ala Gln Trp Leu gat cac gtt ttc Asp His Val Phe ggt acg Sly Thr 255 ttc ggc ggc gtg cgg cac att Phe Gly Gly Val Arg His Ile 260 cag acg gca ttc agt ttg ttt tac tat Gln Thr Ala Phe Ser Leu Phe Tyr Tyr 265 270 WO 01/85772 ctg aaa aac Leu Lys Asn 275 PCT/GBo1/02003 Ctg ott tgg ttt goa ttg cot gog ctg cog otg gog gtt Leu Leu Trp Phe Ala Lou Pro Ala Leu Pro Leu Ala Val tgg aeg gtt tgo cg aog ge ctg ttt tog acc gao tgg ggg att ttg Trp Thr Val Cys Arg Thr Arg Leu Phe Ser Thr Asp Trp Sly Ile Leu 290 295 300 ggo gtc Sly Val 305 gtc tgg atg Val Trp Met goc gtt ttg gtg ctg ott gco gte aat Ala Val Lou Val Lou Lou Ala Val Asn cag egt ttt cag Gin Arg Phe Gin aae ctc gto tgg Asn Lou Val Trp otg Leu 330 tt ccg cg ctt Leu Pro Pro Leu gec ctg Ala Lou 335 1008 tto ggc Phe Gly gcg gcg Ala Ala 340 caa ctg gao agc Gin Leu Asp Ser otg aga cgo Len Aeg Arg 345 tto gga ctg Pho Gly Leu ggo geg gog gog ttt Gly Ala Ala Ala Phe 350 1056 gtc aao tgg ttc Val Asn Trp Phe 355 ggc att atg Sly Ile Met ttt goc Phe Ala 365 gtg tto ctg Val Ph. Leu aag ott gec Lys Lou Ala 1104 tgg acg Trp Thr 370 gge ttt tto gcc Gly Phe Phe Ala aat tao ggo tgg Asn Tyr Gly Trp cc gc Pro Ala 380 1152 ego goc geo tat Arg Ala Ala Tyr ago ccg tat tat Ser Pro Tyr Tyr cct gat ate gat Pro Asp Ile Asp 1200 att cog atg gog gtt goo gta ctg tto Ile Pro Met Ala Val Ala Val Lou Phe 405 cc ttg tgg ctg Pro Lou Txp Lou tgg gcg Trp Ala 415 1248 att ace oge Ile Thr Arg aac ata cgo Asn Ile Arg ggo agg Gly .Arg 425 cag gog gtt acc Gin Ala Val Th sac tgg gcg Asn Txp Ala 430 1296 gca ggo gtt Ala Gly Val 435 aco ctg acc tgg Th Lou Th Trp get ttg Ala Leu 440 otg atg acg ctg tto Otg ccg Leu Met Thr Lou Phe Lou Pro 445 1344 tgg ctg gao gcg gog aaa Tx-p Len Asp Ala Ala Lys 450 ago cac gog ccc gto gte egg agt atg gag ser His Ala Pro Val Val Arg Ser Met Glu 1392 WO 01/85772 PCT/GB01/02003 gca tcg ctt tccccg gaa tta aaa cgg bag ctt tea gac ggq ate gag 1440 Ala Ser Leu Ser Pro Glu Leu Lys Arg Glu Leu Ser Asp Gly Ile Glu 465 470 475 480 tgt atc gac ata ggc gge ggc gac cta cac acg cgg att gtt tgg acg 1488 Cys Ile Asp Ile Gly Gly Gly Asp Leu His Thr Arg Ile Val Trp Thr 485 490 495 cag tac ggc aca ttg ccg cac cge gtc gge gat gta caa tgc cgc tac 1536 Gin Tyr Gly Thr Leu Pro His Arg Val Gly Asp Val Gln Cys Arg Tyr 500 505 510 cgc ate gtc cgc ttg ccc caa aac gcg gat gcg ccg caa ggc tgg cag 1584 Arg Ile Val Arg Leu Pro Gln Asn Ala Asp Ala Pro Gln Gly Trp Gln 515 520 525 acg gte tgg cag ggt gcg cgc ccg ego aac aaa gac agt aag ttc gca 1632 Thr Val Trp Gin Gly Ala Arg Pro Arg Asn Lys Asp Ser Lys Phe Ala 530 535 540 ctg ata cgg aaa ace ggg gaa aat ata tta aaa aca aca gat 1674 Leu Ile Arg Lys Thr Gly Glu An Ile Leu Lys Thr Thr Asp 545 550 555 <210> 2 <211> 558 <212> PRT <213> Neisseria meningitidis <400> 2 Met Leu Thr Tyr Thr Pro Pro Asp Ala Arg Pro Pro Ala Lys Thr His 1 5 10 Glu Lys Pro Trp Leu Leu Leu Leu Met Ala Phe Ala Trp Leu Trp Pro 25 Gly Val Phe Set His Asp Leu Trp ASn Pro Asp Glu Pro Ala Val Tyr 40 Thr Ala Val Glu Ala Leu Ala Gly Ser Pro Thr Pro Leu Val Ala His 55 Leu Phe Gly Gln Ile Asp Phe Gly Ile Pro Pro Val Tyr Leu Trp Val 70 75 Ala Ala Ala Phe Lys His Leu Leu Ser Pro Trp Ala Ala Asp Pro Tyr 90 4 WO 01/85772 Asp Ala Ala Arg Phe 100 Ser Cys Gly Phe Ala 115 Ser Val Val Leu Ile 130 His Phe Leu Asn Pro 145 Leu His Gly Tyr Ser 165 Leu Leu Gly Thr Gly 180 Ala Ala Phe Ala Leu 195 Pro Trp Gin Ser Arg 210 Phe Ala Leu Pro Leu M 225 2 Gln Pro Ala Leu Phe A 245 Phe Gly Gly Val Arg H 260 Leu Lys Asn Leu Leu T 275 Trp Thr Val Cys Arg Ti 290 Gly Val Val Trp Met L 305 31 Gin Arg Phe Gln Asp As 325 Phe Gly Ala Ala Gin Le 340 Ala Gly V Gly Phe A 1; Leu Ile GJ 135 Ala Ala Al 150 Leu Ala Ar Trp Thr Le Met Leu Pr 20 Lrg Leu Mel 215 let Thr Val 30 la Gin Trp is Ile Gin rp Phe Ala 280 ir Arg Leu 295 :u Ala Val .0 :n Leu Val u Asp Ser al Phe Phe Ala Val Val Gly Leu 105 110 sn Phe Leu Gly Arg His His Gly 20 125 Ly Cys Ile Gly Leu Ile Pro Thr 140 .a Ala Phe Ala Ala Ala Gly Leu 155 g Arg Arg Val Ile Ala Ala Ser 170 175 u Met Ser Leu Ala Ala Ala Tyr 185 190 o Leu Pro Val Leu Met Phe Phe 0 205 t Leu Thr Ala Val Ala Ser Leu 220 STyr Pro'Leu Leu Leu Ala Lys 1 235 2 Leu Asp Asp His Val Phe Gly T 250 255 Thr Ala Phe Ser Leu Phe Tyr T 265 270 Leu Pro Ala Leu Pro Leu Ala V 285 Phe Ser Thr Asp Trp Gly Ile Li 300 Leu Val Leu Leu Ala Val Asn P 315 3; Trp Leu Leu Pro Pro Leu Ala Le 330 335 Leu Arg Arg Gly Ala Ala Ala Ph 345 350 PCT/GB01/02003 Thr Arg Val Val 160 Phe Pro Arg Ua hr 'hr yr al eu ro eu [e WO 01/85772 Val Asn Trp 355 Trp Thr Gly 370 Glu Arg Ala 385 Ile Pro Met Ile Thr Arg Ala Gly Val 435 Trp Leu Asp I 450 Ala Set Leu S 465 Cys Ile Asp I Gln Tyr Gly T Arg Ile Val A 515 Thr Val Trp GJ 530 LeU Ile Arg Ly 545 Ph Ph Al Al Lye 42 rTh a er le hr 00 rg Ln s e Gly Ile Met Ala 360 e Phe Ala Met Asn 375 a Tyr Phe Ser Pro 390 a Val Ala Val Leu 405 s Asn Ile Arg Gly 0 Leu Thr Trp Ala 440 Ala Lys Ser His I 455 Pro Glu Leu Lys I 470 Gly Gly Gly Asp L 485 Leu Pro His Arg V 5 Leu Pro Gln Asn A 520 Gly Ala Arg Pro A 535 Thr Gly Glu Asn IJ 550 Phe Gly Leu Tyr Gly Trp Tyr Tyr Val 395 Phe Thr Pro 410 Arg Gln Ala 425 Leu Leu Met Ala Pro Val Arg Glu Leu S 475 ,eu His Thr A 490 al Gly Asp V 05 la Asp Ala P rg Ann Lys A 5 Le Leu Lys T1 555 PCT/GB01/02003 Phe Ala Val Phe Leu 365 Pro Ala Lys Leu Ala 380 Pro Asp Ile Asp Pro 400 Leu Trp Leu Trp Ala 415 Val Thr Asn Trp Ala 430 Thr Leu Phe Leu Pro 445 Val Arg Ser Met- Glu 460 ier Asp Gly Ile Glu 480 Lrg Ile Val Trp Thr 495 al Gln Cys Arg Tyr 510 ro Gln Gly Trp Gin 525 sp Ser Lys Phe Ala hr Thr Asp <210> 3 <211> 1077 <212> DNA <213> Neisseria meningitidis <220> WO 01/85772 <221> CDS <222> (1)..(1077) <400> 3 atg aaa aca ctg acc gta cac acc ccg teec cac age tac Met Lys Thr Leu Thr Val His Thr Pro Ser His Ser Tyr 1 5 10 ate ggc aac gga ctg ctg ccg cag gca gga agc ctg etc Ile Gly Asn Gly Leu Leu Pro Gln Ala Gly Ser Leu Leu 25 ttg gge aaa cge gcc gcc ate atc ace aac gaa ace gtc Leu Gly Lys Arg Ala Ala Ile Ile Thr Asn Glu Thr Val 40 tac etc ggc acg ctt cag acg gca ttg gat gcg gca gge Tyr Leu Gly Thr Leu Gln Thr Ala Leu Asp Ala Aa Gly 55 ttc age atc ate ctg ccc gac ggc gag gcg cac aaa aac Phe Ser Ile Ile Leu Pro Asp Gly Glu Ala His Lys Asn 70 75 ctc aac etc ate ttt gac ggg ctg atg caa aac cge gcc Leu Asn Leu Ile Phe Asp Gly Leu Met Gln ASn Arg Ala C 90 acc acg etc atc gca ctg ggc ggc ggc gtg atc ggc gat a Thr Thr Leu Ile Ala Leu Gly Gly Gly Val Ile Gly Asp M 100 105 1 ttt gcg gcg get act tat caa cgc gge gcg cca ttt gtt c Phe Ala Ala Ala Thr Tyr Gln Arg Gly Ala Pro Phe Val G 115 120 125 act act tta tta agt cag gtc gat tec tcc gtc ggc ggc a Thr Thr Leu Leu Ser Gln Val Asp Ser Ser Val Gly Gly L 130 135 140 ate aac cac ccg etc ggc aaa aat atg ate ggc gcg ttc t Ile Asn His Pro Leu Gly Lys Ahn Met Ile Gly Ala Phe T3 145 150 155 cag gcg gtg ctt gcc gac ttg gac acg ctg cac ace ctg cc Gln Ala Val Leu Ala Asp Leu Asp Thr Leu His Thr Leu Pr 165 170 PCT/GB01/02003 ccc ate ttt Pro Ile Phe aaa cCg cat Lys Pro His gc ccg etc Ala Pro Leu gta tee cat Val Ser His tgg cag acg Trp Gln Thr gaa cgc aaa Glu Arg Lys Itg acg ggt et Thr Gly aa att ccc in Ile Pro aa acec gcc ys Thr Ala ic cag ccg yr Gln Pro 160 :c gcc cge o Ala Arg 175 48 96 144 192 240 288 336 384 432 480 528 WO 01/85772 PCT/GBo1/o2003 gaa ctg tee gee ggt atg gog gas gta ate aaa tac gge aeg etc gge flu Leu Ser Ala Sly Met Ala Glu Val lie Lys Tyr Sly Thr Lou Sly 180 185 190 gac att agt ttt Asp Ile 5cr Phe 195 gca etc gaa cgs Ala Leu Glu Arg 210 ttt gas tgg Phe Glu Trp gcg ccc etc Ala Pro Lou 215 gaa cag cat atg Glu Gin His Met gaa ctg atg Glu Lou Met tgt tge cas Cys Cys Sin atc caa gee gtg Ile Gin Ala Va. tac ege Tyr Arg 220 sag gca gae ate Lys Ala Asp Ile gte gec Val Ala 230 eaa gac gas Gin Asp Glu gas cag ggc ats Glu Gin Sly Ile gca tgg etc aac Ala Trp Lou Asn etc Leu 245 gga cac ace tte Sly His Thr Pho cac gee att gaa His Ala Ile Glu ace gag Thr Glu 255 atg ggt tac Met Sly Tyr ggt act Sly Thr 260 tgg etg eac Trp Lou His gag gee gte gee flu Ala Val Ala gee ggC tge Ala Sly Cys 270 tee gee gca Ser Ala Ala 816 864 gtg ttg geg gcg Val Lou Ala Ala 275 gat ace gcg egg Asp Thr Ala Arg 290 egt ttg te gas Arg Lou Ser Glu 280 caa ctg gge aaa Sin Leu Sly Lys etc gee gee etg etc gas gee Leu Ala Ala Leu Leu Siu Ala 295 gga ctg ccg tee Sly Len Pro Ser gcg Ala 305 cca ccc gtg ttt Pro Pro Val Phe ttt gas aaa tgg ctg Phe Glu Lys Trp Leu 315 gas cac atg age Glu His Met Ser gat asa aaa gte Asp Lys Lys Val gge gge ate atg ege ttt ate ggt etg Sly Sly le Met .Arg Phe lie Sly Leu aae egg Asn Arg 335 etg gge Leu Sly gas gee sac ate ace gaa Sin Ala Asn Ile Thr Glu att acc gae aeg gac ate etc ege Ile Thr Asp Thr Asp Ile Leu Arg 345 350 1008 1056 1077 ege ace etg caa cog tat etc Arg Thr Lou GLn Pro Tyr Leu 355 WO 01/85772 PCT/GB01/02003 <210> 4 <211> 359 <212> PRT <213> Neisseria meningitidis <400> 4 Met Lys Thr Leu Thr Val His 1 1 5 Ile Gly Asn Gly Leu Leu Pro G Leu Gly Lys Arg Ala Ala Ile I Tyr Leu Gly Thr Leu Gin Thr A 55 Phe Ser Ile Ile Leu Pro Asp G: 70 Leu Asn Leu Ile Phe Asp Gly L Thr Thr Leu Ile Ala Leu Gly Gl 100 Phe Ala Ala Ala Thr Tyr Gin Ar 115 12 Thr Thr Leu Leu Ser Gin Val As 130 135 Ile Asn His Pro Leu Gly Lys Al 145 150 Gin Ala Val Leu Ala Asp Leu As] 165 Glu Leu Ser Ala Gly Met Ala Glt 180 Asp Ile Set Phe Phe Glu Trp Leu 195 200 Ala Leu Glu Arg Ala Pro Leu Ile 210 215 9 'hr Pro Ser His 10 ln Ala Gly Ser 25 le Thr Asn Glu 40 la Leu Asp Ala ly Glu Ala His 75 eu Met Gln Asn 90 y Gly Val Ile 105 *g Gly Ala Pro P 0 p Ser Ser Val G 1 n Met Ile Gly A 155 p Thr Leu His T] 170 U Val Ile Lys T' 185 SGlu Gin His Me Gln Ala Val Ty 22 Ser Tyr Leu Leu Thr Val Ala Gly Lys Asn Arg Ala Gly Asp he Val C 125 ly Gly L la Phe T hr Leu P yr Gly TI 15 it Pro G 205 'r Arg Cy 0 Pr Ly 3 Al Va Trj Gli Met I1n rys yr ro hr lu 's :o Ile 's Pro 0 a Pro 1 Ser p Gin a Arg SThr SIle I Thr A Gin P 1 Ala A 175 Leu G Leu Me Cys G1 Phe His Leu His Thr Lys Gly ro la ro rg ly et .n WO 01/85772 Met Lys Ala 225 Ala Trp Leu Met Gly Tyr Val Leu Ala J 275 Asp Thr Ala 7 290 Ala Pro Pro V 305 Asp Lys Lys V Leu Gly Glu A 3, Arg Thr Leu G- 355 PCT/GB01/02003 As Asi Gli 260 Ula Arg al la 40 In p Ile Val Ala Gin Asp Glu Thr Glu 230 235 i Leu Gly His Thr Phe Gly His Ala 245 250 Thr Trp Leu His Gly Glu Ala Val 265 Arg Leu Ser Glu Gin Leu Gly Lys 280 Leu Ala Ala Leu Leu Glu Ala Ala 295 300 Phe Ala Phe Glu Lys Trp Leu Glu I 310 315 Ser Gly Gly Ile Met Arg Phe Ile C 325 330 Asn Ile Thr Glu Ile Thr Asp Thr A 345 Pro Tyr Leu Gln Gly Ile Arg 240 Ile Glu Thr Glu 255 Ala Ala Gly Cys 270 Ile Ser Ala Ala 285 Gly Leu Pro Ser is Met Ser His 320 ;ly Leu Asn Arg 335 .sp Ile Leu Arg 350 <210> <211> 1053 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1053) <400> atg aca cac cat tac ccc aca gac gat att aag att aaa gaa gtt aaa Met Thr His His Tyr Pro Thr Asp Asp Ile Lys Ile Lys Glu Val Lys 1 5 10 gag ttg ttg ccg ccg att gcc cat ctt tac gag ctg ccg att tec aaa Glu Leu Leu Pro Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys 25 WO 01/85772 PCT/GB0102003 gag gct.tcg ggc ttg gtt cac cgc acc cgt cag gaa att tec gat ttg 144 Giu Ala 8cr Gly Lou Val His Arg Thr Arg Gin flu Ile Ser Asp Leu gtt cac ggc Val His Gly agg gac aag cgg ctg ttg gtt att atc ggg ccg tgt tcg Arg Asp Lys Arg Lou Lou Val Ile Ile Gly Pro Cys Ser cac gat cog aaa gog gcg ttg gaa tat gcg gag cgt ttg ttg His Asp Pro Lys Ala Ala Lou Giu Tyr Ala Gin Arg Leu Leu ctc cgc aag cag LeU Arg Lys Gin gaa aac gag ctt ttg att gtg atg egc gtt tat Gu Asn Gin Lou Lou Ile Val Met Arg Val Tyr ttc gag aag ccg Pho Giu Lys Pro 100 ccg cat ttg gac Pro His Lou Asp 115 agg aeg acg gtg Arg Thr Thr Val tgg aa ggt ttg Trp Lys Gly Lu att sac gac Ile Asn Asp 110 ggt acg ttt Sly Thr Phe atc ast ttc ggt Ile Asn Phe Gly ttg cgt cag gca Leu Arg Gin Ala 125 cgc agc ctg ttg Arg Ser Lou Leu 130 ttg tcg ctg aac sat atg ggt atg cct gec tct acc Leu 5cr Lou Asn Asn Met Giy Met Pro Ala Ser Thr 135 gag ttt ttg gat atg att acg ccg caa tat Gin Pho Lou Asp Met Ile Thr Pro Gin Tyr gcg gac ttg att Ala Asp Lou Ile tgg ggg gca atc Trp Gly Ala ILe ggc gcg cgg Gly Ala Arg 165 acg acc gaa age caa gtt cac Thr Thr Gu Ser Gin Val His cgc gaa Arg Glu 175 ttg gca agc Leu Ala Sex ggc sat ttg Gly Asn Leu 195 ggg Gly 180 etg tec tgc ccc gte Leu Ser Cys Pro Val 185 ggc ttt aaa sac ggt acg gat GLy Phe Lys Asn Giy Thr Asp 190 sag att gec Lys Ile Ala stc gac gca Ile Asp Ala 200 ate ggt gcg Ile Giy Ala gcg agc cat tcg Ala Ser His Ser 205 gcc att gtc cat Ala Ile Val His cat cat ttc ctg tct gta ace aag gcc ggg cat His His Phe Lou ser Val Thr Lys Ala Giy His 210 215 WO 01/85772 PCT/GB01/02003 ace ggc ggc aat cct gac tgt cat gtc att ttg cgc ggc gga aaa gag 720 Thr Gly Gly Asn Pro Asp cys His Val Ile Leu Arg Gly Gly Lys Glu 225 230 235 240 ccg aat tat gat gcg gga cac gtc age gag gcg gcg gaa caa ctg cgt 768 Pro Asn Tyr Asp Ala Gly His Val Ser Glu Ala Ala Glu Gin Leu Arg 245 250 255 gcg gca ggc gta ace gac aag ctg atg att gat tgc age cac gcc aac 816 Ala Ala Gly Val Thr Asp Lys Leu Met Ile Asp Cys Ser His Ala Asn 260 265 270 agc cgc aag gat tac ace cga cag atg gaa gtg gcg caa gac att gcc 864 Ser Arg Lys Asp Tyr Thr Arg Gin Met Glu Val Ala Gin Asp Ile Ala 275 280 285 gcc caa ttg gaa cag gac ggc ggc aat att atg ggc gtg atg gtg gaa 912 Ala Gln Leu Glu Gin Asp Gly Gly Asn Ile Met Gly Val Met Val Glu 290 295 300 age cat ttg gtc gaa ggc agg cag gac aag ccg gaa gtg tac ggc aaa 960 Ser His Leu Val Glu Gly Arg Gln Asp Lys Pro Glu Val Tyr Gly Lys 305 310 315 320 agc att acc gat gcg tgt att ggt tgg gac gcg act gaa gaa ctg ttg 1008 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 gca ttg ttg gca ggt gca aac aaa aaa cgt atg gcg cgc gcc ggt 1053 Ala Leu Leu Ala Gly Ala Asn Lys Lys Arg Met Ala Arg Ala Gly 340 345 350 <210> 6 <211> 351 <212> PRT <213> Neisseria meningitidis <400> 6 Met Thr His His Tyr Pro Thr Asp Asp Ile Lys Ile Lys Glu Val Lys 1 5 10 Glu Leu Leu Pro Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys 25 Glu Ala Ser Gly Leu Val His Arg Thr Arg Gin Glu Ile Ser Asp Leu 40 12 WO 01/85772 Val His Gly Arg Ile His Asp Pro Leu Arg Lys Gin Phe Glu Lys Pro 100 Pro His Leu Asp 115 Arg Ser Leu Leu 130 Glu Phe Leu Asp 145 Trp Gly Ala Ile G 1 Leu Ala Set Gly Li 180 Gly Asn Leu Lys I] 195 His His Phe Leu Se 210 Thr Gly Gly Asn Pr 225 Pro Asn Tyr Asp Al 24; Ala Ala Gly Val Th 260 Set Arg Lys Asp Tyr 275 Ala Gin Leu Glu Gin 290 Asp Lys Arg Leu Leu Val 55 Lys Ala Ala Leu Glu Tyr 70 Tyr Glu Asn Glu Leu Leu 90 Arg Thr Thr Val Gly Trp 105 Gly Thr Phe Asp Ile Asn 120 Leu Ser Leu Asn Asn Met 135 let ile Thr Pro Gin Tyr T 150 3 ly Ala Arg Thr Thr Glu S 65 170 eu Set Cys Pro Val Gly P 185 le Ala Ile Asp Ala Ile G 200 r Val Thr Lys Ala Gly Hi 215 o Asp Cys His Val Ile Le 230 23 a Gly His Val Ser Glu Al 5 250 r Asp Lys Leu Met Ile As 265 Thr Arg Gin Met Glu Val 280 Asp Gly Gly Asn Ile Met 295 Ile Ala C 75 Ile V Lys G Phe G. Gly ME 14 'yr A1 .55 er Gl he Ly, ly Ala .s Ser 220 u Arg 5 a Ala Cys l Ala Gly 300 PCT/GB01/02003 Ile Gly Pro Cys Ser lu Arg Leu Leu Lys 'al Met Arg Val Tyr ly Leu Ile Asn Asp 110 ly Leu Arg Gin Ala 125 et Pro Ala Ser Thr L0 .a Asp Leu Ile Ser 160 n Val His Arg Glu 175 s Asn Gly Thr Asp 190 I Ala Ser His Ser 205 Ala Ile Val His Gly Gly Lys Glu 240 Glu Gin Leu Arg 255 Set His Ala Asn 270 Gin Asp Ile Ala 285 Val Met Val Glu WOO01/85772 PCT/GBOI/o2003 Sex His Leu Val Giu Sly Arg Gin Asp Lys Pro Glii Val Tyr Sly Lys 305 310 315 320 Set Ile Thr Asp Mla Cys Ile Sly Trp Asp Mla Thr Giu Giu Lou Leu 325 330 335 Mla Leu Leu Ala Gly Ala Asri Lys Lys Arg Net Ala Arg Ala Sly 340 345 350 <210> 7 <211> 1686 <212> DNA <213> Noisseria meningitidis <220> <221> CDS <222> (1)..(1686) <400> 7 atg tta caa tec gaa aat tcg aaa sat tta atc tct tgt tca ata sag 48 Met Leu Gin 5cr Siu Asa Set Lys Asa Lou Ile Set Cys Set Ile Lys 1 5 10 gct tta cca atc atg att tot ace aac ggc ate ace atg cag ttc gge 96 Ala Leu Pro Ile Met Ile 3cr Thr Asn Gly Ile Thr Met Gin Phe Sly 25 geg aag ceg ctg ttt gaa sac gta tcc gtt aa ttc gge gas ggc ase 144 Ala Lys Pro Lou Phe Glu Asn Vai Set Vai Lys Phe Sly Giu Giy Asn 40 cge tac ggt ttg ate ggc geg sac gge tea ggc ass tco aoc ttc atg 192 Arg Tyr Sly Leu Ile Sly Ala Asn Sly Ser Siy Lys Set Thr Phe Met 55 asa atc ctc ggc ggc gat ttg gas cag acs gee ggc gaa gtg gcg stt 240 Lys Ile Leu Sly Sly Asp Lou Ciu Gin flit Ala Sly Slu VIal Ala Ile 70 75 gas aac ggc gtg cgt ttg ggt aaa ttg cgc caa gac eag ttt gcc tac 288 Giu Asn Giy Vai Arg Lou Sly Lys Leu Arg Gin Asp Gin Phe Ala Tyr 90 gas gat stg age gtg etg gac gtg gta atg atg ggt cac ace gas atg 336 Giu Asp Met Arg Val Lou Asp Vai Val Met Met Sly His Thr Glu Met 100 105 110 14 WO 01/85772 PCT/GB01/02003 tgg gcg gog atg acc gaa cgt gat gcg att tac gc aat ccc gaa gcc Trp Ala Ala Met Thr Glu Arg Asp Ala Ile Tyr Ala Asn Pro Glu Ala 115 1in acc gaa Thr Glu 130 gac gac tac atg aaa goc gca gaa ctg gaa gcc aag ttc gcc Asp Asp Tyr Met Lys Ala Ala Giu Leu Glu Ala Lys Phe Ala tac gac ggc tac Tyr Asp Gly Tyr gcc gaa gcg cgt Ala Glu Ala Arg gea gag ttg ttg Ala Glu Leu Leu 480 ggc gtg ggc att Gly Val Gly lle gaa gat ttg cac Glu Asp Leu His gca ace atg gca Ala Thr Met Ala gaa gtt Glu Val 175 gac cog ggc Ala Pro Gly aaa ctg cgc gta Lys Leu Arg Val ctg gcg caa gcc Leu Ala Gln Ala ctg ttc tcc Leu Phe Set 190 aag cog gat gta Lys Pro Asp Val 195 ttg ctc ttg gac gaa cog acc aat aac ttg gac att Leu Leu Leu Asp Glu Pro Thr Asn Asn Leu Asp Ile at acc Asn Thr 210 atg att Met lle 225 ate cgt tgg ttg gaa gge gtg ttg aac cag tac gac tcc acg Ile Arg Trp Leu Glu Gly Val Leu Asn Gln Tyr Asp Ser Thr 215 220 atc ate tcg cac gac cgc cac tte ttg aac gaa gte tgc acg le Ile Ser His Asp Arg His Phe Leu Asn Glu Val Cys Thr cat atg gca gat His Met Ala Asp ttg gat Leu Asp 245 tac aac acc atc acc atc tat ccg Tyr Asn Thr Ile Thr Ile Tyr Pro 250 ggc aac Gly Asn 255 tac gac gac Tyr Asp Asp aaa gac aat Lys Asp Asn 275 tat atg ctc gcc tcc gcc Tyr Met Leu Ala Ser Ala 260 265 gac aag gcg aag gaa aaa Ala Lys Ala Lys Glu Lys 280 caa tcg cgc gaa Giln Ser Arg GIu cgc gcc ctg Arg Ala Leu 270 caa gaa ttt Gln Glu Phe ttg cag gaa Leu Gln Glu gtg gcg Cgg ttc agt gc a aa aaa tcc aaa goc cgt Val Ala Arg Phe Ser Ala Asn Lys Ser Lys Ala Arg 290 295 300 cag gca acc agc Gin Ala Thr Ser WO 01/85772 PCTIGBOI/02003 ogt ctg aaa cag goa gao aaa ato aaa tog gag atg gto gag gto aaa M-g Leta Lys Gin Ala Asp Lys Ile Lys Ser Glu Met Val Glu Val Lys CCC tot aco cgt Pro 3cr Thi- Arg aec cog tat ato cgt ttt gaa goc gat gaa aaa 1008 Asn Pro Tyr Ile Arg Phe Glu Ala ASP GIU Lys 330 335 goc aag ctg Ala Lys Lou ttt gaa cc Phe Glu Thi- 355 ogb cag gct gtg Arg Gin Ala Val gtt gas ass ctg Val Giu Lys Leu gog aaaocgo Ala Lys Mrg 350 gas gog gga Glu Ala Sly 1056 1104 cag ttg ttt aaa Gin Leu Phe Lys otg sac ttt ato Leu Asn Phe Ile oss cgo Gin Arg 370 Oto goc ato atc Lou Ala Ile Ile cog sac ggc gog Pro Msn Sly Ala ass too aoc otg Lye Her Thi- Leu 1152 as cto ttg gcc Lys Lou Lou Ala ggo gog tao sac coo gaa tat boa gac ggt ctg Sly Ala Tyr Msn Pro Glu Tyr Her Asp Sly Leu 390 395 400 1200 otg oog gao gas ggc Lou Pro Asp Glu Sly 405 ago ato aaa tgg gog gas asa goo 5cr Ile Lye Ti-p Ala GIU Lye Ala 410 tao tat cog Tyr Tyr pro Cas gab Sln Asp 420 cao gaa aao His G-lu Asn cas tgg ggg Gin Ti-p Sly 440 tto gab gto gab Phe Asp Val Asp ago gto ggc Her Val Sly 415 atg gab ttg met Asp Lou 430 gas oas gto Glu Gin Val 1248 1296 1344 ago gaa tgg atg ogo 3cr Giu Ti-p Met Arg 435 cag gao ggo gao gao Gin ASP Sly Asp Asp 445 atc cgo ile Arg 450 ggc act ttg Sly Thi- Lou ggg ogt Sly AMg 455 ttg oto ttC ggc Lou Lou Phe Sly agt aso Her Msn 460 gat gto gtg ASP Val Val 1392 aaa gtg sag gtt Lys Val Lye Val Oto too Lou 5cr 470 ggt ggt gas ass ggo ogt atg ott tao Sly Sly Glu Lys Sly Arg Met Lou Tyr 475 480 1440 1488 ggc aaa ctg ttg ctg ttg aaa coo sat gtc tta gto atg gat gsa cog Sly Lye Lou Lou Lou Lou Lye Pro Msn Val Lou Val Met Asp Glu Pro 485 490 495 WO 01/85772 PCT/GB01/02003 ace aac cac atg gac atg gaa agc ate gaa tcc ttg aac atg gcg ctg 1536 Thr An His Met Asp Met Glu Set Ile Glu Ser Leu Asn Met Ala Leu 500 505 510 gaa aaa tac aac ggc acg ctg att ttc gtc tcce cac gac cgt cag ttc 1584 Glu Lys Tyr Asn Gly Thr Leu Ile Phe Val Ser His Asp Arg Gln Phe 515 520 525 gtt tct.tec ctg gct acc caa ate att gaa ttg gac ggc aaa ggc gga 1632 Val Set Ser Leu Ala Thr Gin Ile Ile Glu Leu Asp Gly Lys Gly Gly 530 535 540 tat gaa cac tac ttg ggc gat tac gaa agc tat ctc gag aaa aaa ggc 1680 Tyr Glu His Tyr Leu Gly Asp Tyr Glu Set Tyr Leu Glu Lys Lys Gly 545 550 555 ,560 gta gca 1686 Val Ala <210> 8 <211> 562 <212> PRT <213> Neisseria meningitidis <400> 8 Met Leu Gln Ser Gli Asn Set Lys Asn Leu Ile Ser Cys Ser Ile Lys 1 5 10 Ala Leu Pro Ile Met Ile Ser Thr Asn Gly Ile Thr Met Gln Phe Gly 25 Ala Lys Pro Leu Phe Glu Asn Val Set Val Lys Phe Gly Glu Gly Asn 40 Arg Tyr Gly Leu Ile Gly Ala Asn Gly Set Gly Lys Ser Thr Phe Met 55 Lys Ile Leu Gly Gly Asp Leu u G Gln Thr Ala Gly Glu Val Ala Ile 70 75 Glu Asn Gly Val Arg Leu Gly Lys Leu Arg Gln Asp Gln Phe Ala Tyr 90 Glu Asp Met Arg Val Leu Asp Val Val Met Met Gly His Thr Glu Met 100 105 110 WO 01/85772 Trp Ala Ala Met 115 Thr Glu Asp Asp 130 Glu Tyr Asp Gly 145 Gly Val Gly Ile Ala Pro Gly Phe 180 Lys Pro Asp Val 195 Asn Thr Ile Arg T 210 Met Ile Ile lie S 225 His Met Ala Asp L 2 Tyr Asp Asp Tyr M< 260 Lys Asp Asn Ala L3 275 Val Ala Arg Phe Se 290 Arg Leu Lys Gin Al 305 Pro Ser Thr Arg GI 32; Ala Lys Leu His Arc 340 Phe Glu Thr Gin Let 355 T T T: Se it 16 Ly Le r] e et 45 et ys ;r a n 5 1 I hr yr M yr T 1 tr G 5 s L u Le p Le c Hi 23 LAs Lei Ale Ala Asp 310 Asn Gin Phe Glu Arg A 1 let Lys A 135 hr Ala G 50 lu Asp L( eu Arg Va eu Leu As 20 u Glu Gl 215 s Asp Ar 0 P Tyr Ash u Ala Set SLys Glu 280 Asn Lys 295 Lys lle Pro Tyr Ala Val Lys Asn 360 18 Lsp Ala Ile Tyr Ala Asn I 20 125 la Ala Glu Leu Glu Ala L 140 lu Ala Arg Ala Ala Glu L 155 eu His Asn Ala Thr Met A 170 l Leu Leu Ala Gin Ala L 185 1i p Glu Pro Thr Asn Asn Le 0 205 y Val Leu Asn Gln Tyr As 220 3 His Phe Leu Asn Glu Va 235 1 Thr Ile Thr Ile Tyr Pr 250 Ala Gin Ser Arg Glu Arc 265 27( Lys Leu Gin Glu Leu Gin 285 Ser Lys Ala Arg Gin Ala 300 Lys Ser Glu Met Val Glu 315 Ile Arg Phe Glu Ala Asp 330 Glu Val Glu Lys Leu Ala 345 350 Leu Asn Phe Ile Leu Glu 365 PCT/GB01/02003 'ro Glu Ala iys Phe Ala eu Leu Ser 160 la Glu Val 175 u Phe Ser )0 !u Asp Ile p Ser Thr 1 Cys Thr 240 3 Gly Asn 255 j Ala Leu I Glu Phe Thr Ser Val Lys 320 Glu Lys 335 Lys Arg Ala Gly WO 01/85772 Gln Arg Leu Al 370 Leu Lys Leu Le 385 Leu Pro Asp Gl Tyr Tyr Pro GI 42' Ser Glu Trp Mel 435 Ile Arg Gly Thr 450 Lys Lys Val Lys 465 Gly Lys Leu Leu Thr Asn His Met 500 Glu Lys Tyr Asn 515 Val Ser Ser Leu 530 Tyr Glu His Tyr 545 Val Ala a Ile Ile Gly Pro Asn Gly Ala Gly Lys Ser Thr 375 380 .u Ala Gly Ala Tyr Asn Pro Glu Tyr Sex Asp Gly 390 395 u Gly ser Ile Lys Trp Ala Glu Lys Ala Ser Val 405 410 415 n Asp His Glu Asn Asp Phe Asp Val Asp Met Asp 0 425 430 t Arg Gin Trp Gly Gin Asp Gly Asp Asp Glu Gn 440 445 Leu Gly Arg Leu Leu Phe Gly Sex Asn Asp Val 455 460 Val Leu Ser Gly Gly Glu Lys Gly Arg Met Leu T 470 475 4 Leu Leu Lys Pro Asn Val Leu Val Met Asp Glu P 485 490 495 Asp Met Glu Ser Ile Glu Ser Leu Asn Met Ala L 505 510 Gly Thr Leu Ile Phe Val Ser His Asp Arg Gln PI 520 525 Ala Thr Gin Ile Ile Glu Leu Asp Gly Lys Gly GJ 535 540 Leu Gly Asp Tyr Glu'Ser Tyr Leu Glu Lys Lys G1 550 555 PCT/GB01/02003 Leu Leu 400 Gly Leu Val ral 'yr ro eu he ly y 0 <210> 9 <211> 1632 <212> DNA <213> Neisseria meningitidis <220> <221> CDS WO 01/85772 <222> (1)..(1632) <400> 9 atg ata aaa cog aac ctg agg Cog aag ctc ggc tct tcc gcg ctg Met Ile Lye Pro Asn Lou Arg Pro Lye Lou Gly Sex Ser Ala Lou 1. 5 10 15 gc tto ctt too ctg tat too tog otg gta ttg aat tao goc ttt Mla Phe Lou Ser Lou Tyr Ser Sex Lou Val Lou Asn Tyr Ala Phe 25 30 9cc aaa gtt gto gag ott cat oct ttt ano ggo aco ggg gog gat Mla Lys Val Val GlU Lou His Pro Phe Asn.G(ly Thr Gly Ala Asp 40 45 ttc oto tat acg atg ccg gtg gtg ctg ttt ttt tta agt aat tto Pho Lou Tyr Thr Met Pro Val Val Lou The Phe Lou Sex Aen Phe 55 60 ttt cac gto att gcc ctg cot tto gtg cat aag gta ttg att cogI Phe His Val Ile Mla Lou Pro Phe Val His Lye Val Lou Ile Pro 1 70 75 ata ttg gtt ato agt gog gcg gtg tot tao caa gaa ata ttt tto a Ile Lou Val Ile 8cr Mla Mla Val. Ser Tyr Gin Glu Ile Phe Pho 90 95 atc tat ttc aac aag tcg atg ttg aat ant gtc ttg can act aog g Ile Tyr Phe Asn Lys Sor Met Lou Ann Ann Val Lou Gin Thr Thr A 100 105 110 gco gaa ago gog ogo otg att aog ccg ggo tat gtg ctg tgg att gi Mla Gin 3cr Ala Arg Lou Ile Thr Pro Gly Tyr Val Lou Trp Ie V 115 120 125 tgt ttg ggo gta ttg ccc gog otg gcg tat etc goc gtc aag gtt as Cys Lou Gly Val Lou Pro Ala Leu Ala Tyr Ile Ala Val Lye Vai Ly 130 135 140 tao 090 gtt tgg tat aag gag ott ttg aog ogo ott gtg ott gcc go Tyr Arg Val Trp Tyr Lys Glu Leu Lou Thr Arg Lou Val. Lou Mla Al 145 150 155 16 gtt too ttt ttg tgo gog ttg ggo ato gon atg ttg ena tat cag gal Vai 3cr Pho Lou Cys Mla Leu Gly Ile Mla Met Lou Gin Tyr Gin Asj 165 170 175 tao goc tcg ttt tto 090 aao aat aaa ton gta aco cat otg att gtc PCT/GBO01/200a att Ile ttt Pho ato le gtt Vai e~u at zn ct la ti 'a 0 t 288 432 480 528 WO 01/85772 Tyr Ala Set cca tct nat Pro Ser Asn 195 cgt tcc aat Arg Ser Asn 210 cgc ccg gccc Arg Pro Ala 225 acc acg Cgt g Thr Thr Arg A acg ccg ctg c' Tin Pro Lou L gtc aga ago tcj Val Arg Set cN 275 tca acc ttc go Tin Phe As 290 gac aac ctg ct Asp Asn Lou Lel 305 ttg gao aac gat Lou Giu Asn Asp aco gac gtt cc Thr Asp Vol Thr 340 tgc ctc gac ant Cys Lou Asp Asn 355 aac gat aa gac PCT/GBOI/o200J Ph. Ph. Arg Asn Asn Lys Ser Vol Thr His Lou Ile Vol 180 185 190 ttc ntc ggc gcg ggc gtg tcg aoa tao ann gat tgg nag 624 Ph. Ile Gly Ala Gly Val Sot LYS Tyr Lys Asp Trp Lys 200 205 att cct tot acg can ttg gat atg gcg gtc gtg caa aac 672 l. Pro Tyr Thr Gin Leu Asp Met Aln Vol Vai Gin Asn 215 220 rgc ago ctg cgc ogt ttc gtg gtg ctg gtc gtg ggc gag 720 ly 8cr Len Arg Arg Ph. Vol Vai Lou Val Vol Gly Giu 230 235 240 cc go ano tgg ggt ttg aoc ggt tac agc cgc coa act 768 la Ala Asn Trp Gly Lou Asn Gly Tyr 8cr Arg Gin Thr 245 250 255 tt gcc gcg cgc ggc gat gaa att gtc aat tto cog cog 816 iu Ala Ala Arg Gly Asp Giu Ile Val Asn Phe Pro Gin 265 270 *c ggt aca tcg ncc gog cac tcc ctg ccg tgt atg ttc 864 *a Gly Thr 5cr Thr Ala His 5cr Lou Pro Cys Met Ph. 280 285 c cgc aeg gat tat gac gna atc aaa gcc gao coo caa 912 p Mrg Thr Asp Tyr Asp Giu Ile Lys Ala Giu His Gin 295 300 g gac atc gtg cog cgc gcc ggo gtg gao gtt acc tgg 960 u1 Asp Ile Vai Gin Mrg Ala Gly Vol Giu Vol Thr Trp 310 3159 320 too ggc tgc nag ggc gtg Ego ggc aaa gtg ccg oat 1008 Ser Gly Cys Lys Gly Val Cys Gly Lys Val Pro Asn 325 330 335 tcg ctc oac ctg ccc gao tac tgc ogc ao ggc gag 1056 Ser Lou Asn Lou Pro Giu Tyr Cys Arg Asn Gly Glu 345 350 ato ctg ctg acc ang Etc gac gaa gtc ctc aoc oaa 1104 Ile Lou Lou Thr Lys Phe Asp Giu Val Lou Asn Lys 360 365 gog gtt tta atc ctg cat acc oto ggc ago cac ggg 1152 21 WO 01/85772zC/B0/20 Asn ASP Lys Asp Ala Val. Len Ile Leu His Thr Ile Gly Ser His Gly 370 375 380 sog tat tao gas Thr Tyr Tyr Giu cgc tat scc gaa gcc gas cgc ass ttc scg cog Arg Tyr Thr Gin Ala Gin Arg Lys Phe .Thr Pro 390 395 400 1200 soc tgc: gao cc Thr Cys Asp Thr aso gaa ato sac ass tgc Ass Giu Ile Asn Lys Cys 405 410 Soc ogo gcc Thr Arg Ala scg ctg gtc Thr Leu Val 415 atc gac ag Ile ASP Lys 1248 act tao Ass Tin Tyr sat acg gtt ttg Asn Thr Val Len gtg gac cag ttt Val Asp Gin Phe 1296 gtt sto Val Ile tat gtt. Tyr Val 450 ass ott gas sac Lys Len Gin Asn gao gat ttg gas ASP Asp Leu Gin agc gtg gtg cat 8cr Val. Val His 445 ggg atg tao ctg Gly Met Tyr Len 1344 too gao cac ggc 8cr ASP His Sly gsa agt 455 ttg ggo Len Gly ga sac Gin Asn 460 1392 900 gag oct, tao Ala Ala Pro Tyr goc ato gog oct too Ala le Ala Pro 5cr 470 ggg cag Gly Gin 475 ag cat ato Thr His le 1440 atg gtt stg tgg Met Vai Met Trp too aa goc ttc 8cr Lys Ala Phe cas oso ggg ggo Gin is Gly Gly ata gat Ile Asp 1488 tto as tgc Phe Gin Cys cac tat Etc His Tyr Ph-e 515 ass cas ass gog Lys Gin Lys Ala gas sac gas Giu Ass Giu tat tog oao gao Tyr 8cr His Asp 1536 ago aog gta ttg ggg otg atg gao Scr Thr Val Len Gly Len Met Asp 520 atE too sac ago aa Ile set Ass Ser Gin 1584 UOO tat cg9 sag gas atg gat ata ttg gos goc tgo ogo ogt cog ogoc Thr Tyr Arg Lys Gin Met Asp Ile Len Ala Ala Cys Arg Arg Pro Arg 530 535 540 <210> <211> 544 <212> PRT <213> Neisseria meningitidis 1632 WO 01/85772 <400> Met Ile Lys Pro Asn Leu 1 5 Ala Phe Leu Ser Leu Tyr Ala Lys Val Val Glu Leu Phe Leu Tyr Thr Met Pro Phe His Val Ile Ala Leu 70 Ile Leu Val Ile Ser Ala Ile Tyr Phe Asn Lys Ser 1 100 Ala Glu Ser Ala Arg Leu I 115 Cys Leu Gly Val Leu Pro A 130 1: Tyr Arg Val Trp Tyr Lys G 145 150 Val Ser Phe Leu Cys Ala Le 165 Tyr Ala Ser Phe Phe Axg As 180 Pro Ser Asn Phe 11e Gly Al 195 Arg Ser Asn Ile Pro Tyr Th 210 21' Arg Pro Ala Gly Ser Leu Arc 225 230 Thr Thr Arg Ala Ala An Trp PCT/GB01/02003 Arg Pro Ser Ser His Pro 40 Val Val 55 Pro Phe Ula Val let Leu 1 le Thr P 120 la Leu A 35 lu Leu Li u Gly Il n Asn Ly 18 a Gly Va 200 r Gln Lei I Arg Phc Gly Leu 23 I P L V Se .0 ar e eS 5 1 u e Lys Leu Gly Ser 10 eu Val Leu Asn 25 he Asn Gly Thr G eu Phe Phe Leu S al His Lys Val L 75 Ir Tyr Gin Glu If 90 :n ASn Val Leu G1 5 o Gly Tyr Val Le 12 a Tyr Ile Ala Va 140 u Thr Arg Leu Val 155 Ala Met Leu Gln 170 Ser Val Thr His Ser Lys Tyr Lys 205 Asp Met Ala Val 220 Val Val Leu Val 235 Asn Gly Tyr Ser Ser Ala Leu Ile ryr Ala Phe Phe ly Ala Asp Ile er Asn Phe Val eu Ile Pro Leu Le Phe Phe Asn .n Thr Thr Ala 110 u Trp Ile Val 1 Lys Val Lys L Leu Ala Ala 160 Tyr Gln Asp 175 Leu Ile Val 190 Asp Trp Lys Val Gln Asn Val Gly Glu 240 Arg Gln Thr WO 01/85772 PCT/GB01/02003 Thr Val A Ser T 2 Asp A 305 Leu G Thr As Cys Le Asn As 37 Pro Th 385 Thr Cys Asn Thr Val lle Tyr Val 450 His Ala 465 Met Val Phe Gln 245 Pro Leu Leu Ala 260 Lrg Sex Cys Gly 275 hr Phe Asp Arg 90 sn Leu Leu Asp lu Asn Asp Ser 325 5p Val Thr Ser I 340 ru Asp Asn Ile L 355 p Lys Asp Ala v 0 r Tyr Tyr Glu A 3! Asp Thr Asn GJ 405 Tyr Asp Asn Th 420 Arg Lys Leu Gl 435 Ser Asp His GI' Ala Pro Tyr Al 47( Met Trp Phe Ser 485 Cys Leu Lys Gin T T I 3. G1 Le ie lu Ir u y a 0D 250 Ala Arg Gly Asp Glu Ile Val 265 Yhr Ser Thr Ala His Ser Leu 280 hr Asp Tyr Asp Glu Ile Lys 295 300 le Val Gln Arg Ala Gly Val 10 315 ly Cys Lys Gly Val Cys Gly 330 u Asn Leu Pro Glu Tyr Cys 345 u Leu Thr Lys Phe Asp Glu 360 3 1 Leu Ile Leu His Thr Ile G 375 380 g Tyr Thr Glu Ala Glu Arg L 0 395 Ile Asn Lys Cys Thr Arg A 410 SVal Leu Tyr Val Asp Gln P, 425 Asn Arg Asp Asp Leu Glu Se 440 44 Glu Ser Leu Gly Glu Asn Gl 455 460 Ile Ala Pro Ser Gly Gln Th: 475 Lys Ala Phe Arg Gln His G15 490 Lys Ala Ala Glu Asn Glu Tyr 24 255 ASn Phe Pro Gin 270 Pro Cys Met Phe 285 Ala Glu His Gln Glu Val Thr Trp 320 Lys Val Pro Asn 335 Arg Asn Gly Glu 350 ral Leu Asn Lys ly Ser His Gly ys Phe Thr Pro 400 la Thr Leu Val 415 he lle Asp Lys 430 r Val Val His y Met Tyr Leu r His Ile Pro 480 Gly Ile Asp 495 Ser His Asp WO 01/85772 PCT/GB01/02003 500 505 510 His Tyr Phe Ser Thr Val Leu Gly Leu Met Asp Ile Ser Asn Ser Gln 515 520 525 Thr Tyr Arg Lys Glu Met Asp Ile Leu Ala Ala Cys Arg Arg Pro Arg 530 535 540 <210> 11 <211> 1052 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1050) <400> 11 atg aca cac cat tac ccc aca gac gat att aag att aaa gaa gtt aaa 48 Met Thr His His Tyr Pro Thr Asp Asp Ile Lys Ile Lys Glu Val Lys 1 5 10 gag ttg ttg ccg ccg att gcc cat ctt tac gag ctg ccg att tcc aaa 96 Glu Leu Leu Pro Pro Ile Ala His Leu Tyr Glu Leu Pro Ile Ser Lys 25 gag get tcg ggc ttg gtt cac cgc acc cgt cag gaa att tcc gat ttg 144 Giu Ala Ser Gly Leu Val His Arg Thr Arg Gln Glu Ile Ser Asp Leu 40 gtt cac ggc agg gac aag cgg ctg ttg gtt att atc ggg ccg tgt tcg 192 Val His Gly Arg Asp Lys Arg Leu Leu Val lle Ile Gly Pro Cys Ser 55 att cac gat ccg aaa gcg gcg ttg gaa tat gcg gag cgt ttg ttg aaa 240 Ile His Asp Pro Lys Ala Ala Leu GIu Tyr Ala Glu Arg Leu Leu Lys 70 75 80 880 ctc cgc aag cag tat gaa aac gag ctt ttg att gtg atg cgc gtt tat 288 Leu Arg Lye Gin Tyr Glu Asn Glu.Leu Leu Ile Val Met Arg Val Tyr 90 ttc gag aag ccg agg acg acg gtg ggt tgg aaa ggt ttg att aac gac 336 Phe Glu Lys Pro Arg Thr Thr Val Gly Trp Lys Gly Leu lie Asn Asp 100 105 110 WO 01/85772 PCT/GBOI/02003 ceg cat ttg gac ggt aeg ttt gac etc eat ttc ggt ttg cgt cag gee Pro His Lou Asp Gly Thr Phe Asp Ile Aen Plie Gly Lou Arg Gin Ala Cgc ego Arg Set 130 ctg ttg ttg tog ctg LOU Lou Lou Set LeU 135 aac eat etg ggt atg cot gee tet ace Aan Asn Met Gly Met Pro Mla Set Thr 140 ttt ttg gat Phe Lou Asp atg att Met Ile 150 acg ccg cee tat Thr Pro Gin Tyr geg gac ttg att Ala Asp Lou Ile tgg ggg gca ate Trp Gly Mla Ile gge gcg egg Giy Mla Arg 165 aeg eec gee Thr Thr Glu 170 agc cee gtt cac Cgc gae Set Gin yal His Arg Giu 175 ttg gce ago Lou Mla ser ctg tee tgo ccc Lou Set Cya pro ggc ttt ace eec Gly Phe Lys Aen ggt acg get Gly Tbr Asp 190 age cat tog 3cr His Ser ggc eat ttg aeg att gee ate Giy Asn LOU Lys Ile Ala Ile gca ate ggt geg Mla Ile GlY Ale eat cat His His 210 tte ctg tet gte Plie LOU Her Val eag goc ggg cat Lys Mla Gly His gee att gtc cat Ala Ile Val. His 624 672 720 ggc ggo aet Gly Gly Asn cet gee Pro Asp 230 tgt cat gtc ett Cys His Val Ile ogc gge gga aaa Arg Gly Giy Lys ceg aat tat get geg gga cac gte egc Pro Asn Tyr Asp Ala (fly His Vai Set 245 geg gcg gee cee Mla Ma- Giu Gin etg egt Lou Arg 255 geg gee gge Ale Mla rfly age cgc aag Set Arg Lys 275 ace gac eag ctg atg att get tgc agc Thr Asp Lys Lou met Ile Asp Cys Set 265 cec gec aec His Ala Asn 270 gac att gee Asp Ile Ala gat tee aec cge ASP Tyr Thr Arg cag etg Gin Met 280 gee gtg gcg Giu Val Ala gec caa ttg gaa ceg giac gge gge eat att atg gge gtg atg gtg gaa Ale Gin Lou Glu Gin Asp Gly Gly Asn Ile Met Gly Vel Met Val Giu 290 295 300 WO 01/85772 PCT/GB01/02003 agc cat ttg gtc gaa ggc agg cag gac aag cog gaa gtg tac ggc aaa 960 Se His Leu Val Glu Gly Arg G l n Asp Lys Pro Glu Val Tyr Gly Lys 305 310 315 320 agc att ace gat gcg tgt att ggt tgg gac gcg act gaa gaa ctg ttg 1008 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 gca ttg ttg gca ggt gca aac aaa aaa cgt atg gcg cgc gcc gg 1052 Ala Leu Leu Ala Gly Ala Ash Lys Lys Arg Met Ala Arg Ala 340 345 350 4 5 350 <210> 12 <211> 350 <212> PRT <213> Neisseria meningitidis <400> 12 Met Thr His His Tyr Pro Thr Asp A 1 5 Gl Leu Leu Pro Pro Ile Ala His Le 2 Glu Ala Ser Gly Leu Val His Arg Th 40 Val His Gly Arg Asp Lys Arg Leu Lei 55 Ile His Asp Pro Lys Ala Ala Leu Gli 70 Leu Arg Lys Gin Tyr Glu Asn Glu Leu Phe Glu Lys Pro Arg Thr Thr Val Gly 100 105 Pro His Leu Asp Gly Thr Phe Asp Ile 115 120 Arg Se3 Leu Leuu Le L Ser Leu Asn Asn 130 135 Glu Phe Leu Asp Met Ile Thr Pro Gln 145 150 27 ;p lie Lys Ile Lys Glu Val Lys 10 lu Tyr Glu Leu Pro Ile Ser Lys 5 r Arg Gin Glu Ile Ser Asp Leu u Val Ile Ile Gly Pro Cys Ser 1 Tyr Ala Glu Arg Leu Leu Lys 75 Leu lie Val Met Arg Val Tyr 90 Trp Lys Gly Leu Ile Asn Asp 110 Asn Phe Gly Leu Arg Gin Ala 125 Met Gly Met Pro Ala Ser Thr 140 Tyr Tyr Ala Asp Leu Ile Ser 155 160 WO 01/85772 PCT/GB01/02003 Trp Gly Ala le Gly Ala Arg Thr Thr Glu Ser Gin Val His Arg Glu 165 170 175 Leu Ala Sex Gly Leu Ser Cys Pro Val Gly Phe Lys Asn Gly Thr Asp 180 185 190 Gly Asn Leu Lys Ile Ala Ile Asp Ala Ile Gly Ala Ala Set His Ser 195 200 205 His His Phe Leu Set Val Thr Lys Ala Gly His Ser Ala Ile Val His 210 215 220 Thr Gly Gly Asn Pro Asp Cys His Val Ile Leu Arg Gly Gly Lys Glu 225 230 235 240 Pro Asn Tyr Asp Ala Gly His Val Ser Glu Ala Ala Glu Gin Leu Arg 245 250 255 Ala Ala Gly Val Thr Asp Lys Leu Met Ile Asp Cys Ser His Ala Asn 260 265 270 Set Arg Lys Asp Tyr Thr Arg Gin Met Glu Val Ala Gin Asp Ile Ala 275 280 285 Ala Gin Leu Glu Gin Asp Gly Gly Asn Ile Met Gly Val Met Val Glu 290 295 300 Set His Leu Val Glu Gly Arg Gin Asp Lys Pro Glu Val Tyr Gly Lys 305 310 315 320 Ser Ile Thr Asp Ala Cys Ile Gly Trp Asp Ala Thr Glu Glu Leu Leu 325 330 335 Ala Leu Leu Ala Gly Ala Asn Lys Lys Arg Met Ala Arg Ala 340 345 350 <210> 13 <211> 432 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> WO 01/85772 PCT/GBOI/o2003 <400> 13 atg gos ttt ggt tog atg ast too ggc gao gat tot cog atg too gao 48 Met Ala Phe Gly 5cr Met Asn 5cr Sly ASP Asp 5cr Pro met 5cr Asp 1 5 10 ato aso gtt sog cog ttg gtg gac gtg atg ctg gtg ttg ctg att gtg 96 Ile Asn Val Thr Pro Leu Val Asp Val Met Len Val Lou Leu Ile Val 25 ttt. atg att act atg ocg gtg otg aog cat too ato ct ttg gaa ctg 144 Phe Met Ile Thr Met Pro Val Lou Thr His Set Ile Pro Leu Glu Lou 40 ccg aoc gog too gag cag aos sac sag cag gac ass cag cot ass gao 192 Pro Thr Ala 5cr Giu Gin Thr Asn Lys Gin Asp Lys Gin Pro Lys Asp 55 000 ctg cgc ctg sog att. gat gcg sac ggc g tat tat gtc ggc ggg 240 Pro Lou Arg Lou Thr Ile Asp Ala Asn Sly Gly Tyr Tyr Val Sly Gly 70 75 gat tot gca agc aaa gtg gsa stc ggg gsa gtg gsa agc cgt ctg aaa 288 Asp Ser Ala Set Lys Val Gil Ile Sly Gin Val Gin Ser Arg Lou Lys 90 9cc goc aag gag cag aat gsa sac gtg att gtg gog stt 909 gca gao 336 Ala Ala Lys Glu Gin Asn Glu Ann Val Ile Val Ala Ile Ala Ala Asp 100 105 110 sag gog gtg gaa tao gat tat gta aac Baa got tta gas gco gcc cgt 384 Lys Ala Val (flu Tyr Asp Tyr Val Ann Lys Ala Lou Gin Ala Ala Arg 115 120 125 c59 gca gga sto acc ass a to ggt ttt gts aoc gsa 500 sag gog cas 432 Gin Ala Sly Ile Thr Lys Ile Sly Phe Val Thr Sic Thr Lys Ala Gin 130 135 140 <210> 14 <211> 144 <212> PRT <213> Noissoria moningitidis <400> 14 Met Ala Phe Sly 5cr Met Asn 5cr Sly Asp Asp 5cr Pro Met 5cr Asp 1 5 10 Ice Asn Val Thr Pro Lou Val Asp Val Met Lou Vai Lou Lou Ile Val 29 WO 01/85772 PCT/GB01/02003 Phe Met Ile Pro Thr Ala Pro Leu Arg Asp Ser Ala Ala Ala Lys Lys Ala Val C 115 Gin Ala Gly I 130 Thr Met Pro Val Leu Thr His Set Ile Pro Leu Glu Leu 40 Ser Glu Gln Thr Asn Lys Gln Asp Lys Gln Pro Lys Asp 55 Leu Thr Ile Asp Ala ARn Gly Gly Tyr Tyr Val Gly Gly 70 75 Ser Lys Val Glu Ile Gly Glu Val Glu Ser Arg Leu Lys 90 Glu Gln Asn Glu Asn Val Ile Val Ala Ile Ala Ala Asp 100 105 110 lu Tyr Asp Tyr Val Asn Lys Ala Leu Glu Ala Ala Arg 120 125 le Thr Lys Ile Gly Phe Val Thr Glu Thr Lys Ala Gln 135 140 <210> <211> 267 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> atg ctc aaa caa tcc atc ga a atatc aac aaa ctc gga ctc cac gcc Met Leu Lye Gln Set Ile Glu Ile Ile Asn Lys Leu Gly Leu His Ala 1 5 10 cgc gcg tcc aac aag ttc acc caa acc gcg tcc caa ttc aaa agc gaa Arg Ala Ser Asn Lys Phe Thr Gln Thr Ala Ser Gin Phe Lys Ser Glu 25 gtc tgg gtt acg aaa aac gac agc cgc gtc aac ggc aaa agc att atg Val Trp Val Thr Lys ASn Asp Ser Arg Val Asn Gly Lys Sex Ile Met 40 ggg ctg atg atg ctc gcc gcc gcc aag ggt acg gtc ate gas ctg gag WO 01/85772 PCT/GB01/02003 Sly Leu Met Met Leu Ala Ala Ala Lys Gly Thr Val Ile Glu Leu Glu 55 acg gac ggc gcg gac gag gcg gaa gcg atg cgc gcc ctg acc gac tta 240 Thr Asp Gly Ala Asp Glu Ala Glu Ala Met Arg Ala Leu Thr Asp Leu 70 75 atc aac gge tac ttc ggc gag ggc gaa 267 Ile Asn Gly Tyj Phe Gly Glu Gly Glu 267 <210> 16 <211> 89 <212> PRT <213> Neisseria <400> 16 Met Leu Lys Gin 1 Arg Ala Set Asn Val Trp Val Thr Gly Leu Met Met Thr Asp Gly Ala Ile Asn Gly Tyr meningitidis Ser Ile Glu Ile Ile Asn Lys Leu 5 10 Lys Phe Thr Gln Thr Ala Ser Gln 25 Lys Asn Asp Ser Arg Val Asn Gly 40 Leu Ala Ala Ala Lys Gly Thr Val 55 Asp Glu Ala Glu Ala Met Arg Ala 70 75 Phe Gly Glu Gly Glu Gly Leu His Ala Phe Lys Set Glu Lys Ser Ile Met Ile Glu Leu Glu Leu Thr Asp Leu <210> 17 <211> 633 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 17 WO 01/85772 PCT/GBOI/o200J atg ccc atg aac ctt ttt caa aac goc aaa ttc ttc aog acg gta aac 48 Met Pro Met Ann Lou Phe Gin Ann Ala Lys Phe Phe Thr Thr Val Ann 1 5 10 cat ctc ana gac ctg ccg gac aca cog otc gas att gcc ttt gtc ggc 96 His Lou Lys Asp Lou Pro Asp Thr Pro Lou Glu Ile Ala Phe Vol Sly 25 agg ago aat gc gga aaa too agE gc atc aat aco ctg aco aac cat 144 Arg Ser Asn Ala Gly Lys Ser Ser Ala Ile Ann Thr Leu Thr Ann His 40 gtc cgt ott gco tac Val Arg Lou Ala Tyr gtt ta aa aca ccc gga cgg acg cag cat ato Val Ser Lys Thr Pro Gly Arg Tb: Gin His Ile cag aac ggc aat ttt atg gto gat ttg coo ggc Gin Ann Giy Asn Phe Met Val Asp Lem Pro Gly 70 75 aao An tto ttc gag ctg The Phe Glu Leu tac ggt tat goc Tyr Gly Tyr Ala caa gtc ccc Gin Val Pro gao goa gta Ogc gco cat Glu Ala Vol Arg Ala His 90 Egg gtc at Trp Val Asn ctg ctc ggc Leu Lou Giy ttg ott atg Leu Ile Met 115 gao tat ctg caa Asp Tyr Leu Gin 100 cag ogc aaa cog ott atc Gin Arg Lys Gln Lou Ile 105 99 ctg gtt Sly Lou Val 110 otc cgt atg lie Arg Met got goc cgc cat Asp Ala Arg His cct tta aaa gaa ctc gao Pro Lou Lys Glu Lou Asp 120 125 384 ctg gac Len Asp 130 ttt Etc cac acg Phe Phe His Thr ggc aga cog gtt Sly Arg Pro Val gc gao aaa Eta Ala Asp Lys Leu oaa aac gaa cag Lys Ann Glu Gin cac atc ctg ctg eca His Ile Len Lou Ser 140 aaa aco ctg too cao Lys Tb: Leu Ser Gin 160 caa aac atc agc gta Gin Ann Ilc Ser Val 175 gtc aaa aaa ctg Val Lys Lys Leu aaa cot tat Lys Pro Tyr too gac egg Ser Asp Arg 170 cag Otg ttt too ago ctg aaa aaa cao ggt ott gao gag goc ooo oga Gin Len Pho 5cr Sc Lou. Lys Lys Gin Sly Ile Asp Glu Ala An Arg 180 185 190 WO 01/85772 PCT/GB01/02003 .act gtc gga agc tgg ttt gac gca gca gat gcc gcc gct tcc tct cca 624 Thr Val Gly Set Trp Phe Asp Ala Ala Asp Ala Ala Ala Ser Set Pro 195 200 205 aag gaa aac Lys Glu Asn 633 210 <210> 18 <211> 211 <212> PRT <213> Neisseria meningitidis <400> 18 Met Pro Met Ann Leu Phe Gln Asnn Ala Lys Phe Phe Thr Thr Val Asn 1 5 10 His Leu Lys Asp Leu Pro Asp Thr Pro LeU Giu Ile Ala Phe Val Gly 25 Arg Ser Asn Ala Gly Lys Set Ser Ala Ile Asn Thr Leu Thr Asn His 40 Val Arg Leu Ala Tyr Val Set Lys Thr Pro Gly Arg Thr Gln His Ile 55 Asn Phe Phe Glu Leu Gln Asn Gly Asn Phe Met Val Asp Leu Pro Gly 70 75 Tyr Gly Tyr Ala Gln Val Pro Glu Ala Val Arg Ala His Trp Val Asn 90 Leu Leu Gly Asp Tyr Leu Gin Gln Arg Lys Gln Leu Ile Gly Leu Val 100 105 110 Leu Ile Met Asp Ala Arg His Pro Leu Lys Glu Leu Asp Ile Arg Met 115 120 125 Leu Asp Phe Phe His Thr Thr Gly Arg Pro Val His Ile Leu Leu Ser 130 135 140 Lys Ala Asp Lys Leu Ser Lys Asn Glu Gln Ile Lys Thr Leu Ser Gln 145 150 155 160 Val Lys Lys Leu Leu Lys Pro Tyr Ser Asp Arg Gin Asn TIle Se 165 170 S al 175 WO 01/85772 PCT/GB01/02003 Gin Leu Phe Ser ser Leu Lys Lys Gln Gly Ile Asp Glu Ala Asn Arg 180 185 190 Thr Val Gly Ser Trp Phe Asp Ala Ala Asp Ala Ala Ala Ser Ser Pro 195 200 205 Lys Glu Asn 210 <210> 19 <211> 621 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (621) <400> 19 atg aaa cga ttg act tta ttg gcc ttt gtt ttg got gcc ggt gcg gtt 48 Met Lys Arg Leu Thr Leu Leu Ala Phe Val Leu Ala Ala Gly Ala Val 1 5 10 tcc gca tot ccc aaa gca gac gtg gaa aaa ggc aaa cag gtt gcc gca 96 Ser Ala Ser Pro Lys Ala Asp Val Glu Lys Gly Lys Gln Val Ala Ala 25 acg gtt tgt gcg got tgc cat gca gca gac ggt aac ago ggc att gcg 144 Thr Val Cys Ala Ala Cys His Ala Ala Asp Gly Asn Ser Gly Ile Ala 40 atg tat ccg cgt ttg gcg gca cag cat act got tac atc tat cat caa 192 Met Tyr Pro Arg Leu Ala Ala Gln His Thr Ala Tyr Ile Tyr His Gln 55 acc atc ggc atc cgc gac ggt aaa cgc acc cac ggt tcg gca gct gtg 240 Thr Ile Gly Ile Arg Asp Gly Lys Arg Thr His Gly Stcr Ala Ala Val 70 75 atg aaa ccg gtg gta atg aat ttg agc gat cag gat att ttg aac gta 288 Met Lys Pro Val Val Met Asn Leu Set Asp Gin Asp Ile Leu Asn Val 90 tcc gca ttc tat gcc aaa cag cag ccc aaa tcc ggt gaa gcc aat cct 336 Ser Ala Phe Tyr Ala Lys Gln Gln Pro Lys Setr Gly Glu Ala Asn Pro 100 105 110 34 WO 01/85772 WO 015772 PCT/GB01/02003 aag gaa aat ccc gaa ttg ggt gcg aaa atc tat cgc ggc ggt ttg age 384 Lys GIu Asn Pro Glu Leu Gly Ala Lys Ile Tyr Arg Gly Gly LeU Set 115 120 125 gat aaa aaa gtg ccg gcg tgt atg tcc tgc cac ggt cog ago ggt gcg 432 Asp Lys Lys Val Pro Ala Cys Met Sex Cys His Gly Pro Sex Gly Ala 130 135 140 ggt atg cog ggg ggc gga agc gaa att cag gct tat ccg cgt ttg ggc 480 ly Met Pro ly Gly Gly Ser Glu Ile Gln Ala Tyr Pro Arg Leu Gly 145 150 155 160 ggt cag cat cag gca tat att gtt gaa cag atg aat gcc tac aag too 528 Gly Gln His Gin Ala Tyr Ile Val Glu Gin Met Asn Ala Tyr Lys Ser 165 170 175 ggt cag cgt aaa aat ace atc atg gaa gat att gca aac cgt atg tot 576 Gly Gln Arg Lys Asn Thr Ile Met Glu Asp Ile Ala Asn Arg Met Ser 180 185 190 gaa gas gat ttg aaa gcg gtc gcc aac ttt atc caa ggt ttg cgt 621 Glu GluAsp Leu Lys Ala Val Ala Asn Phe Ile Gln Gly Leu Arg 195 200 205 <210> <211> 207 <212> PRT <213> Neisseria meningitidis <400> Met Lys Arg Leu Thr Leu Leu Ala Phe Val Leu Ala Ala Gly Ala Val 10 Ser Ala Sex Pro Lys Ala Asp Val Glu Lys Gly Lys Gln Val Ala Ala 25 Thr Val Cys Ala Ala Cys His Ala Ala Asp Gly Asn Ser Gly Ile Ala 40 Met Tyr Pro Arg Leu Ala Ala Gln His Thr Ala Tyr Ile Tyr His Gln 55 Thr Ile Gly Ile Arg Asp Gly Lys Arg Thr His Gly Ser Al Ala Val 70 75 Met Lys Pro Val Val Met Asn Leu Ser Asp Gln Asp Ile Leu Asn Val WO 01/85772 PCT/GBOI/02003 Sex Ala Phe Tyr 100 Lys Glu Asn Pro 115 ASP Lye Lye Val 130 Sly Met Pro Sly 145 Sly Sin His Gin Gly Gin Arg Lys 180 Giu (flu Asp Lou L 195 Mla Lys Giu Lou Pro Mla Sly Gly 150 'Ua Tyr 165 \sn Thr Ys Mla I Gn G.in Prc 105 Sly Ala Lys 120 Cys Met Set 135 Set Glu Ile Ile Val Giu Ile Met Giu 185 Valn Ala Asn 200 Lys Sex Sly Giu Ala Ann Pro 110 le Tyr Arg Gly Gly Lou 125 Cys His Giy Pro 8cr Gly Mla 140 Gin Mla Tyr Pro Arg Lou Sly 155 160 -Gin Met Asn M-a Tyr Lys 8cr 170 175 Asp -Ile Mla Asn Arg met 190 Phe Ile Gin Sly Leu Arg 205 <210> 21 <21i> 765 <212> DNA <213> Neisseria meningitijjs <220> <221> cn)s <222> <400> 21 atg cog tot gaa gcc oga ttt t Met Pro 8cr Giu Mla ArgPhe I 1 5 ttg att tgc ggt ata atc ego c Lou Ile cys ly Ile Ile Arg L. atg aca cgo aaa ato etc gtt a, Met Thr Arg Lys Ile Lou Val T1 ago ate cac etc ggc cac atg gt agg ott cag aeg gca ttt ccc Arg Lou Sin Thr Mla Phe pro 10 tac oca ttg ttt gca nag cac Tyr Pro Lou Pho Ala Lys His 25 toe goc ctg ccc tat goc nac 8cr Ala Lou Pro Tyr Ala Azn gaa cac ate can aco gac gtt egt Arg ant Asn gge Sly tgg WO 01/85772 PCT/GBOI/ozoo3 Bar Ile His Leu Gly His Met Val Gin His Ile Gin Tin Asp Val Trp 55 gtg cgo ttt can aaa otg cgo ggc aac gaa tgc cac tao tgc tgc gOO 240 Val Arg Phe Gin Lys Leu Arg Gly Asn Glu Cys His Tyr Cys Cys Ala 70 75 gao gao aco cac ggc acg ccc gtg atg ctt gco gog caa aan caa ggc 288 Asp Asp Thr His Gly Thr Pro Val Met Lou Mla Ala Gin Lye Gin Gly 90 atc gcg ccc gaa gao atg att goc aaa gtg ogo gaa gag cac cic goc 336 Ie Mla Pro Gin Asp Met Ile Ala Lys Val Arg Gin Gin His Lou Mla 100 105 110 gao ttt act ggc ttt tto ato ggt tac gac aat tat tac ago aco cat 384 Asp Phe Thr Gly Phe Phe Ile Gly Tyr Asp Asn Tyr Tyr Set Thr His 115 120 125 too cot gaa aac aaa cag ttt too oaa gao att tao ogo gog otg aaa 432 Ser Pro Giu Asn Lys Gin Phe Ser Gin Asp Ile Tyr Arg Mla Lou Lys 130 135 140 goc aao ggo nag att gag ago ogo gto etc gag cag ott tto gao 000 480 Mla Asm Gly Lys Ile Gin Ser Arg Val Ile Gin Gin Lou Phe Asp Pro 145 150 155 160 gaa aaa ona atg ttc otg 000 gao cgc tto gtt aan ggo gaa tgc cog 528 Gin Lye Gin Met Phe Len Pro Asp Arg Phe Vai Lys Gly Gin Cys Pro 165 170 175 aaa tgo cac goc can gao can tao ggo gao ano tgc gaa gto tgo ggc 576 Lye Cys His Ala Gin Asp Gin Tyr Gly Asp Asn Cys Gin Val Cys Gly 180 185 190 aog aco tat too cog aco gan ctg att ano cog tat too goc gtt toc 624 Thr Thr Tyr Ser Pro Thr Giu Len Ile Asn Pro Tyr Set Mla Val Ser 195 200 205 ggo gog ann coo gaa ttg ogo gaa too gaa cac tto ttc tto aaa otg 672 Gly Ala Lys Pro Giu Len Arg Gin Ser Gin His Phe Phe Phe Lys Len 210 215 220 gga gan tgo goc gao ttc oto aaa gca tgg act too ggo aao aao cog 720 Gly Gin Cys Mla Asp PMe Lou Lye Ala Tip Thr Set Gly Aen Asn Pro 225 230 235 240 oao gao ggc aag coo cat ctg can goc gaa goc oto aac aan atg 765 37 WO 01/85772 PCT/GB01/02003 His Asp Gly Lys Pro His Leu Gin Ala Glu Ala Leu Asn Lys Met 245 .250 255 <210> 22 <211> 255 <212> PRT <213> Neisseria meningitidis <400> 22 Met Pro Ser Glu Ala Arg Phe I 1 5 Leu Ile Cys Gly Ile Ile Arg I Met Thr Arg Lys Ile Leu Val T Se Ilie His Leu Gly His Met V 55 Val Arg Phe Gln Lys Leu Arg G 70 Asp Asp Thr His Gly Thr Pro V; lie Ala Pro Glu Asp Met Ile A] 100 Asp Phe Thr Gly Phe Phe Ile G1 115 12 Ser Pro Glu Ann Lys Gin Phe Se 130 135 Ala Asn Gly Lys lie Glu Ser Ar 145 150 Glu Lys Gin Met Phe Leu Pro As] 165 Lys Cys His Ala Gin Asp Gin Tyl 180 Thr Thr Tyr Ser Pro Thr Glu Leu 195 200 38 P 4 a r a g pI he Arg Leu Gin 10 eu Tyr Pro Leu 25 ir Ser Ala Leu 10 1r Glu His Ile y Asn Glu Cys 75 1 Met Leu Ala 90 a Lys Val Arg 105 STyr Asp Asn T Gin Asp Ile T 1 Val Ile Giu G 155 Arg Phe Val L 170 Gly Asp Asn C 185 Ile Asn Pro TV T P] P3 G1 6 Hi ;ii y 'yr In ys ys rr hr Ala Phe Pro Arg he Ala Lys His Asn :o Tyr Ala Asn Gly .n Thr Asp Val Trp ;0 s Tyr Cys Cys Ala a Gln Lys Gln Gly u Glu His Leu Ala 110 r Tyr Ser Thr His 125 Arg Ala Leu Lys Leu Phe Asp Pro 160 Gly Glu Cys Pro 175 Glu Val Cys Gly 190 Ser Ala Val Ser 205 WO 01/85772 PCT/GBOI/o2003 Gly Ala Lys Pro Gin Lou Arg Gin 5cr Gin His Phe Phe Phe Lys Len 210 215 220 Gly Gin Cys Ala Asp Phe Lou Lys Ala Trp Thr Set Gly Asn Asn Pro 225 230 235 240 His Asp Gly Lys Pro His Lou Gin Ala Gin Ala Lou Ann Lys Met 245 250 255 <210> 23 <211> 1539 <212> DNA <213> Neissoria meningitidis <220> <221> C0s <222> (1539) <400> 23 atg sag ttt tta gac cgt gag goa sgc stt goc sag cog ggt ttc sac 48 Met Lys Phe Lou Asp Arg Giu Ala Se Ile Ala Lys Pro Gly Phe An 1 5 10 cgt tgg ctc gta ccg ccc gos goa ttg gog gta cac ctt gcc atc ggg 96 Arg Trp Lou Val Pro Pro Mla Ala Lou Mla Val His Lou Ala Ile Gly 25 cag stt tac gcc tat tcc gts ttc sac gog cog ctg acc ana ctt ato 144 Gin Ile Tyr Ala Tyr Set Val Phe Asn Ala Pro Lou Thr Lye Lou Ile 40 ggc ata acc gsa tcg gog goc gga gat tgg aag ctg aog acc gta ggt 192 Gly Ile Thr Gin Sex Ala Ala Gly Asp Trp Lys Lou Thr Thr Val. Gly 55 tgg att ttc agt atc gos ctc gog stg ctg ggc gog tog gcc gcc ctg 240 Trp Ile Pbs 8cr Ile Ala Lou Ala Mot Lou Gly Mla 8cr Ala Ala Lou 70 75 ttc ggt aog tgg atg gaa cgg gta ggg cog cgo aaa gao ata ttt gcc 288 Pho Gly Thr Trp Met Giu Arg Val Gly Pro Jvrg Lys Ala Ile Phe Ala 90 gcc go tgc tgt ttc ago ctg ggo ttc ttc gta toe gca ttc ggc gtg 336 Als Ala Cys Cys Phe Set Lou Gly Phe Pho Val 5cr Ala Phe Gly Val 39 WO 01/85772 PCTIGBO1Io200S cgt aeg cac Arg Thr His 115 aac ctc ttc ctg ctc tat ttg Cgc aac ggc gtc atc ggc Asn Len Phe Lou Len Tyr'Lou Gly Asn Gly Val Ile Gly ggc gta ggo ttg ggt ttg ggc tac ato ggg ccg gtt Gly Val Gly Lou Gly Len Gly Tyr Ile Gly Pro Val 130 135 140 too aca ctg atg 3cr Thr Lou Met t99 ttt ccc gac Trp Phe Pro Asp ccg ggt atg gcg Pro Gly Met Mla ggt ttg gcg att Gly Len Mla Ile ggt ttc ggt ggc Gly Phe Gly Gly ggc gca atg ctg 9cc tcg Gly Ala Met Len Ala 8cr 165 170 ccg ctt tco gta Pro Leu Ser Val tca ctg Ser Lou 175 atg aac aco Met Asn Thr 9cc gta ttg Ala Val Lou 195 aoc atc ogc Thr Ile Arg 210 tca aac got act 3cr Asn Ala Thr gtt 999 gtt gcc Val Gly Val Ala gaa aco ttc Gin Thr Phe 190 ggo gcg ttt Gly Ala Phe ggt ctg Ctt tac Gly Leu Len Tyr 9ca ctg atg atg ttc Ala Lou Met Met Phe 205 gta oct gcc Va1 Pro Ala ggc tgg aaa Cot Sly Trp Lys Pro ggc tat aco gcg Gly Tyr Thr Ala aaa aog oaa aac Lys Thr Gin Asn aag ctg gtc Lys Lou Val 230 ago ago ast Her Her Asri 235 cat gtc aac gtg His Val Asn Val. caa 9cc atg aaa Gin Ala Met Lys cog cag ttt tgg Pro Gin Phe Trp ttg ttc tgg gta Leu Phe Trp Val ttg tgo Len Cys 255 atg aac gta Leu Asn Val act gcc Thr Ala 260 ggc ato ggc Gly Ile Sly ttg ggt cag gca Lou Sly Gin Ala too gtg atg 3cr Val Met 270 att cag gaa ctc ttt toc gaa acc too Ile Gin Giu Leu Phe 3cr Giu Thr Her 275 280 ata ggc agg cag gog gca gtc Ile Giy Arg Gin Ala Ala Vai 285 ggc gca ggt gcg gcg gca 990 ttc gtc ago Cto ctg ago otg ttt aac Sly Ala Gly Ala Mla Ala Giy Phe Val Ser Lou Lou Her Len Phe Asn WO 01/85772 PCT/GBOI/0200s atg ggo gga cgc ttt ttg tgg tog agc gtt tee gao aaa ate gga cgo Met Gly Sly Arg Pie Leu Trp Ser Ser Val Ser Asp Lys Ile Sly Arg 21 n CZ aaa aat act tat Lye Asn Thr Tyr atc ttc ttc gta Ile Pie Phe Val ggt tcg ctg Gly Ser Leu gcc gtt ccc Ala Val Pro ggc ttt tgc Sly Phe Cys 355 atc ggc gag ggc Ile Sly Glu Sly age aaa gee ctg Ser Lys Ala Leu otg tat tto Leu Tyr Phe 335 ttc ate ate Phe Ile Ile 350 got gcc att Ala Ala Ile 1008 1056 1104 gtg att att Val Ile Ile tec atg 3cr Met 360 tat ggc ggc gga Tyr Sly Sly Sly CCC gee Pro Ala 3.70 tat ctg aaa gac Tyr Leu Lye Asp ttc ggc act tat Phe Sly Thr Tyr gtc ggc geg att Val Gly Ala Ile gga cgc ate ctg Sly Arg Ile Leu gcc tgg tca ace Ala Trp Ser Thr gee gtc atc ggc Ala Val Ile Sly 1152 1200 1248 gta etg gte aat Val Leu Val Asn. gcg gea cag gca Ale Ala Sin Ala 420 att atc ggt ttg Ile Ile Sly Leo 435 ata cgo caa ago Ile Arg Gin Ser ate gao agc ggc Ile Asp Ser Gly ata ccg Ile Pro 415 tat agc gta act Tyr Ser Val Thr tao ate atg gcg Tyr Ile Met Ala ggt ctg ctg Sly Leu Leo 430 cat gaa ass His Glu Lys 1296 1344 ttg tgc aac Leo Cys Asn gcc gtc aaa tcc Ala Val Lye Sex cat cat His His 450 gaa aaa gao atc Glu Lye Asp Ile aco gcc gca tac Thr Ala Ala His ggc aac coo gat Sly Ase Pro Asp 1392 gaa ace gee ata Glu Thr Ala Ile tee gac got tat ctt gtg ggc gas sac gtt 3cr Asp Ala Tyr Leo Val Sly Glu Lye Val 470 475 1440 1488 ggt ggo ggc ata teg gtc tgg tgg egt tgg gca ttg gcc gte ata ecg Sly Sly Siy le 3cr val Trp Trp Arg Trp Ala Leu Ala Val Ile Pro WO 01/85772 PCT/GB01/02003 485 490 495 ctc gcc tac ggc gtg gtg atg atc ttt gtc aaa gca etc gac ctt ttc 1536 Leu Ala Tyr Gly Val Val Met Ile Phe Val Lys Ala Leu Asp Leu Phe 500 505 510 tSe 1539 Ser <210> 24 <211> 513 <212> PRT <213> Neisseria meningitidis <400> 24 Met Lys Phe Leu Asp Arg Glu Ala Ser Ile Ala Lys Pro Gly Phe Asn 1 5 10 Arg Trp Leu Val Pro Pro Ala Ala Leu Ala Val His Leu Ala Ile Gly 25 Gin lie Tyr Ala Tyr Ser Val Phe Asn Ala Pro Leu Thr Lys Leu Ile 40 Gly Ile Thr Glu Ser Ala Ala Gly Asp Trp Lys Leu Thr Thr Val Gly 55 Trp Ile Phe Ser Ile Ala Leu Ala Met Leu Gly Ala Ser Ala Ala Leu 70 75 Phe Gly Thr Trp Met Glu Arg Val Gly Pro Arg Lys Ala ile Phe Ala 90 Ala Ala Cys Cys Phe Ser Leu Gly Phe Phe Val Ser Ala Phe Gly Val 100 105 110 Arg Thr His Asn Leu Phe Leu Leu Tyr Leu Gly Asn Gly Val Ile Gly 115 120 125 Gly Val Gly Leu Gly Leu Gly Tyr Ile Gly Pro Val Ser Thr Leu Met 130 135 140 Lys Trp Phe Pro Asp Lys Pro Gly Met Ala Thr Gly Leu Ala Ile Met 145 150 155 160 Gly Phe Gly Gly Gly Ala Met Leu Ala Ser Pro Leu Ser Val Ser Leu 165 170 175 42 WO 01/85772 Met Asn Thr Phe 180 Ala Val Leu Gly 195 Thr Ile Arg Val 210 Pro Lys Thr Gin 225 Gin Ala Met Lys Leu Asn Val Thr A 260 Ile Gln Glu Leu P 275 Gly Ala Gly Ala A 290 Met Gly Gly Arg P2 305 Lys Aan Thr Tyr Th 32 Ala Val Pro Ser Ii 340 Gly Phe Cys Val 11 355 Pro Ala Tyr Leu Ly 370 His Gly Arg Ile- Leu 385 Val Leu Val Asn Tyr 405 Ala Ala Gin Ala Tyr 420 i P T' 2 h h .5 e e s Ser Asn Leu Leu ro Ala Sn Lys 230 hr Pro G 45 la Gly I te SeX G a Ala G 25 e Leu Tr 310 r Ile Ph Gly Gl Ile Ser Asp Leu 375 Leu Ala 390 Ile Arg Sex Val Ala Thr Ser 185 Tyr Leu Ala 200 Asp Gly Trp 215 Leu Val Ser an Phe Trp le Gly Val I 265 lu Thr Ser I 280 Ly Phe Val S 5 p Ser Ser Vi e Phe Val L 33 u Gly Gly Se 345 Met Tyr Gl 360 Phe Gly Th Trp Ser Thr Gln Ser Gin 410 Thr Met Tyr 425 43 Val Gly Leu Met Lys Pro Ser Asn I 235 Leu Leu P 250 Leu Gly G le Gly A er Leu Le 30 al Ser As 315 u Gly Se 0 r Lys Al y Gly Gy13 r Tyr Gin 380 Ala Ala 395 Ile Asp Ile Met PCT/GB01/02003 Val Ala Glu Thr Phe 190 Met Phe Gly Ala Phe 205 Glu Gly Tyr Thr Ala 220 His Val Asn Val Sex 240 'he Trp Val Leu Cys 255 in Ala Set Val Met 270 rg Gin Ala Ala Val 285 eu Ser Leu Phe Asn 0 p Lys Ile Gly Arg 320 r Leu Leu Tyr Phe 335 a Leu Phe Ile Ile 350 Phe Ala Ala Ile 365 Val Gly Ala Ile Val Ile Gly Pro 400 Ser Gly Ile Pro 415 Ala Gly Leu Leu 430 WO 01/85772 PCT/GB01/02003 Ile Ile Gly Leu Leu Cys Asn Leu Ala Val Lys Ser Val His Glu Lys 435 440 445 His His Glu Lys Asp Ile Lys Thr Ala Ala His Ser Gly Asn Pro Asp 450 455 460 Asp Glu Thr Ala Ile Ser Asp Ala Tyr Leu Val Gly Glu Lys Val Ser 465 470 475 480 Gly Gly Gly Ile Ser Val Trp Trp Arg Trp Ala Leu Ala Val Ile Pro 485 490 495 Leu Ala Tyr Gly Val Val Met Ile Phe Val Lys Ala Leu Asp Leu Phe 500 505 510 Set <210> <211> 1038 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1038) <400> ttg cag cct tta gtc agc gta ttg att tgc gcC tac aac gta gaa aaass Leu Gin Pro Leu Val Ser Val Leu Ile Cys Ala Tyr Asn Val Glu Lys 1 5 10 tat ttc gcc caa tca tta gcc acc gtc gtg aat cag act tgg cgc aac Tyr Phe Ala Gin Ser Leu Ala Thr Val Val Asn Gln Thr Trp Arg Asen 25 ttg gag att ttg att gtc gat gac ggo tcg aca gac ggt acg ctt gcc Leu Glu Ile Leu Ile Val Asp Asp Gly Set Thr Asp Gly Thr Leu Ala 40 att gcc aag gat ttt caa aag cgg gac agc cgt atc aaa atc ctt gca Ile Ala Lys Asp Phe Gin Lys Arg Asp Set Arg Ile Lys Ile Leu Ala 55 caa gct ca aat tcc ggc ctg att ccc tct tta aac ate ggg ctg gac 44 48 96 144 192 240 WO 01/85772 PCTIGBOI,0200 3 Ginl A5la Gin Asn Ser Gly Leu Ile Pro 3cr Leu Asn Ile Gly Lou Asp 70 75 ga ttg gcs sag toa gga atg ggg gsa tat att gon ego ace gat 9cc 8 Gin Lou Ala Lys Ser Gly Met Gly Giu Tyr le Ala Arg flit Asp Ala 28 90 gac gat att gee gcc coo gao tgg att gag asa ate gtg ggc gag atg 336 Asp Asp Ile Ala, Ala Pro ASP Trp le Gin Lys Ile Val Gly Gin Met 100 105 110 gaa aas gac ogo ago ato sto gog atg ggo ac g t a t t 384 Giu Lys Asp Arg Se Ilie ile Ala Met Gly A a Trp Lou Giu a l Lentt 115 120 125 tog gas gaa sag gao ggc aso cgg ckg geg cgg cat 080 sgg cac ggo 432 Set Gin Gin Lys Asp Gly Asn Arg Leu Ala Arg His His Arg His Gly 130 135 140 ass att tgg as nag cog 800 ogg eac gas gat att gee gae ttt tto 480 Lys Ile Trp Lys Lys Pro Thr Arg His Gin Asp Ile Ala Asp Phe Phe 145 150 155 160 cot ttc ggc sac coo sta cay eac sac sog atg att atg egg ege ago 528 pro Phe Gly Asn Pro Ile His Asrn As n Thr Met le Met Arg Arg Set 15170 175 gto atE gao g gga ttg ogt tac sac aoc gag ogg gat Egg geg gas 576 Val Ile Asp Gly Gly Lou Arg Tyr Ass Thr Gin Arg Asp Trp Ala Gin 180 .185 190 gat tao as ttt tgg tac gat gto ago ass tt 9 ggc sgg otg got tat 624 Asp Tyr Gin Phe Trp Tyr Asp Val 5cr Lye Lou Gly Arg Lou Ala Tyr 195 200 205 tat ccc gas goc ttg gtc ass tao ogo ctt oso goc sat cag gtt ta 672 Tyr Pro Glu Ala Leu Val Lys Tyr Arg Lou His Ala Ass Gin Val Set 210 215 220 tec ass tao ago atE c 90 085os gsa ate gog osa 990 ste 088 888 720 Set Lye Tyr Set Ile Arg Gin His Gin Ile Ala Gin Gly Ile Gin Lys 225 230 235 240 ace goc Aga sac gat ttt ttg cag tot atg ggt ttt an 800 ogg ttc 768 Thr Ala Arg Asn Asp Phe Lou Gin Set Met ely Phe Lys Thr Arg Phe 245 250 255 gao ago ott gsa tac ego as ats an gcs gts gog tat gas ttg otg 816 WO 01/85772 PCT/GB01/02003 Asp Ser Leu Glu Tyr Arg Gln Ile Lys Ala Val Ala Tyr Glu Leu Leu 260 265 270 gag aaa cat ttg ccg gaa gaa get ttt gaa cgc gcc cgc cgg ttt ttg 864 Giu Lys His Leu Pro Glu Glu Asp Phe Glu Arg Ala Arg Arg Phe Leu 275 280 285 tac caa tgc ttc aaa cgg acg gac acg cog ccc gcc ggc gcg tgg ctg 912 Tyr Gln Cys Phe Lys Arg Thr Asp Thr Pro Pro Ala Gly Ala Trp Leu 290 295 300 gat ttc gcg gca gac ggc.aag atg agg cgg ctg ttt acc atg agg caa 960 Asp Phe Ala Ala Asp Gly Lys Met Arg Arg Leu Phe Thr Met Arg Gin 305 310 315 320 tac ttc ggc att ttg cac cgg ctg att aaa aac cgc cgg cag gcg cgg 1008 Tyr Phe Gly Ile Leu His Arg Leu Ile Lys Asn Arg Arg Gln Ala Arg 325 330 335 tcg gat tcg gca ggg aaa gaa cag gag att 1038 Ser Asp Set Ala Gly Lys Glu Gin Glu Ile 340 345 <210> 26 <211> 346 <212> PRT <213> Neisseria meningitidis <400> 26 Leu Gln Pro Leu Val Set Val Leu Ile Cys Ala Tyr Asn Val Glu Lys 1 5 o10 Tyr Phe Ala Gln Ser Leu Ala Thr Val Val Asn Gin Thr Trp Arg Aan 25 Leu Glu lle Leu Ile Val Asp Asp Gly Ser Thr Asp Gly Thr Leu Ala 40 Ile Ala Lys Asp Phe Gin Lys Arg Asp Ser Arg Ile Lys Ile Leu Ala 55 Gln Ala Gin Asn Set Gly Leu Ile Pro Ser Leu Asn Ile Gly Leu Asp 70 75 Glu Leu Ala Lys Ser Gly Met Gly Glu Tyr Ile Ala Arg Thr Asp Ala 90 WO 01/85772 Asp Asp tle Ala Ala Pro Asp T rp Ile Glu Lys 100 105 Glu Lys Asp Arg Ser Ile Ile Ala Met Gly Ala 115 120 Ser GiU Glu Lys Asp Gly Asn Arg Leu Ala Arg 130 135 Lys Ile Trp Lys Lys Pro Thr Arg His Glu Asp 145 150 155 Pro Phe Gly Asn Pro Ile His Asn Asn Thr Met 165 170 Val Ile Asp Gly Gly Leu Arg Tyr An Thr Glu I 180 185 Asp Tyr Gin Phe Trp Tyr Asp Val Ser Lys Leu G 195 200 Tyr Pro Glu Ala Leu Val Lys Tyr Arg Leu His A 210 215 2 Set Lys Tyr Set Ie Arg Gln His Glu Ile Ala G. 225 230 235 Thr Ala Arg Asn Asp Phe Leu Gln ser Met Gly P1 245 250 Asp Set Leu Glu Tyr Arg Gin Ile Lys Ala Val Al 260 265 Glu Lys His Leu Pro Glu Glu Asp Phe Glu Arg Al 275 280 Tyr Gln Cys Phe Lys Arg Thr Asp Thr Pro Pro Al 290 295 30 Asp Phe Ala Ala Asp Gly Lys Met Arg Arg Leu PhE 305 310 315 Tyr Phe Gly Ile Leu His Arg Leu Ile Lys Asn Arg 325 330 Ser Asp Set Ala Gly Lys Glu Gln Glu Ile 340 345 Ile Val Trp Leu 125 His His A 140 Ile Ala A Ile Met A g Asp T ly Arg Le 205 la Ann Gl ln Gly 11 he Lys Th .a Tyr Glu 270 a Arg Arg 285 a Gly Ala 0 e Thr Met Arg Gln PCT/GB01/02003 Gly Glu Met 110 Glu Val Leu Lrg His Gly sp Phe Phe 160 rg Arg Sex 175 rp Ala Glu U! Ala Tyr n Val Ser e Gin Lys 240 r Arg Phe 255 SLeu Leu Phe LeU Trp Leu Arg Gin 320 Ala Arg 335 WO 01/85772 PCT/GBoJ/o200s <210> 27 <211> 1856 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1857) <400> 27 atg cca gaa tac cgc Met Pro Giu Tyr Arg 1 5 ggc gog ego gog ttg Gly Ala Arg Ala Leu tee aaa ace tee ace cac ggt egc Oat atg gcg 3cr Lys Thr 8cr Thr His Gly Arg Asn Met Ala tgg age gc Trp Arg Ala gge gtg atg gas Gly Val Met Giu aec gac Etc Thr Asp Phe 9gc asa cog Ott etc gee gtE gcc Gly Lys Pro Ile Ile Ale Val Ala A sac beg Etc ace cas ttc gtg eca Asia 5cr Phe Thr Gin Phe Val Pro ggt cat Gly His so gte cee ctg cee Val His Leu His atg ggCc g ctg gtt gcc ege gas atc Met Gly Gin Leu Val Ala Arg Glu Ile aee gee tte sac acc etc gee abc gee Lys Glu Phe DAn Thr Ile Ala Ile Asia sea gee ggc gee Lys Ala Gly Ala atc gc Ile Ala gec qg ate get ASP Gly Ile Ala gga cac agc ggt Gly His Sex Gly Ctg tat toc ctg Leu Tyr 9cr Leu tee agc Pro cgc get btg Arg Asp Leu gcc gee tcc ate Ala Asp Sex Ile bet etg gtc Oat Tyr Met Val DAn gcc eec tgc Ala His Cys 110 acc ccg gge Thr Pro Gly gee gee gcg ctg gtg tgc att Ala Asp Ala Leu Val Cys Ile 115 eec Egc gac ae DAn Cys Asp Lys atg etg atE gce geg atg Met Leu Ie Ala Ala Met 130 ege etg eac ate ceg ace ate ttc gtt tee Arg Leu Asia Ile Pro Thr Ile Phe Vel.Ser 135 WO 01/85772 PCT/GBOI/02003 ggc ggt acg atg gaa gaa ggt eaa gtt ate ggc gtg gca aac atc cag 480 Gly Gly Pro Met Glu Ma Gly Lys Val Ile Gly Val Ala Awn Ile Gin 145 150 155 160 ccc gaa cgc cgt Pro Giu Arg Arg gac ttg att gec gag etg ett Asp Lou Ile Asp Ma Met tie ga tag gag gac Glu So: Ale Asp 175 caa aac gee tgc Gin Awn Ale Cyw 190 gac eat ate Asp Asn Ile acg ace tgc Pro Thr Cys 195 aac Cgg caa gtt Asn Arg Gin Vhi ga gta gee Glu Val Giu ggc tag tgt Gly Ser Cyw tag ggt atg Ser Gly Met 200 ttt acg gce Phe Thr Ae aac tag etg eec Asn Ser Met Asn 205 tgc ctg ace gaa gca atc Cys Lou Thr Glu Ala Lou 210 etc gc ace cec gta ggc Leu Ala Thr His Val Gly 225 ggg Gly 215 ctg tee ctg ccg Leu Ser Lou Pro ggc aac ggt tat tac Gly Awn Gly Ser Tyr 220 etc gee gc ggc cgt Leu Glu Ala Giy Arg cgc aaa gaa ctg Arg Lys Gu Leu etg ett.gtc gee Met Ile Val Glu aca aea cgc tat Thr Lys Arg Tyr tac gag Tyr Glu 250 caa aac gat Gin Awn Asp gaa ace Glu Thr 255 gtg tte cag Val Lou Pro acc atg gac Thr Met Asp 275 aga att gac Ser Ile Ale ace aaa Thr Lyw 265 aaa gag ttt gee aac gat etg Lys Ale Pho Gu An Ala Met 270 ace aac act att ttg cat tta Thr Asn Thr Ile Lu His Leu 285 ett gc atg gga ggc ego Ile la Met Gly Gly 8er 280 864 atc gac Leu Ale 290 gtt gac aec gaa Vai Ale Awn Glu gga gtg get tte aaa atg gce gec ate Gly Vel Asp Pho Lys Met Mea Asp Ile 300 gac Asp 305 ego tta age cgc Arg Leu Se: Arg gtt gtg ccc tgc Val Val Pro Cys 310 ete tgc aaa Ile Cys Lys 315 aca gca 6ac aac Thr Ala Pro Asn 320 aac cac gaa tac tat atg gee gec gtg cac cga gac ggc gga Asn His Asp Tyr Tyr Met Glu Asp Vel His Arg Ala Gly Gly atc ttc Ile Phe 335 1008 WO 01/85772 PCTIGBOI/0200S gee atc ctg Ala le Leu aaa gas ttg gac aaa geg ggc aag ctg eac ace gac gtg Lye Glu Lcu Asp Lys Ma Sly Lye Leu His' Thr Asp Val 340 345 350 cac act ate cac geg cog aeg ctg aaa gao gcg His Thr Ile His Ala Pro Tin Leu Lye Asp Ala 355 360 att gaa Ile Glu 365 aaa tgg gac Lye Trp Asp 1056 1104 1152 gta acc Val Thr 370 sac oct gas aac ace cac gee ate gas cgo tte asa gec gcg Asn Pro Glu Ass Thr His Ala Ile Glu Arg Phe Lys Ala Ala ggc ggo gta Sly Sly Val ego ace Arg Thr 390 acc caa gcg tte tog caa aac cgt atg Thr Gin Ala Phe 3cr Gin Asn Arg Met 395 1200 aaa ace ctc gao Lye Thr Leu Asp etc gac cgo Leu Asp Arg 405 gaa aaa ggo Siu Lys Sly 410 tgt atc cgc gac Cys Ile Arg Asp gtg gca Vai Ala 415 1248 1296 cac gee tee His Ala Tyr gee gag cgo Ala Glu Arg 435 caa gao gge ggt Gin Asp Sly Gly gg gte ttg ttc Ala Val Le Phe gge aac ate Sly Asn Ile 430 gag age atc Glu Ser ile gge tgc gtg gta aaa ace ges gge gtg Sly Cys Val Val Lye Thr Ala Gay Val 440 1344 ctc aaa Len Lye 450 tte ace gge cgo gee go gtg ttt gaa Phe Thr Gly Arg Ala Arg Val Ph, Gin 455 caa gaa gac gca Sin Glu Asp Ala 1392 gae ggc att Glu sly Ile ttg gge aac Leu Gly Ass 470 caa ate gte Gin ile Val ggc gac ate gte Sly Asp Ile Val 1440 atc cg tac gas Ile Ag Tyr Glu gge cog aaa gge ggc ceg Sly Pro Lys Gly Sly Pro 485 490 gge atg cag gas Sly Met Gin Glu atg ctg Met Len 495 1488 tat cog act Tyr Pro Thr tac ctg aaa tot Tyr Len Lye Ser saa gge Lye Sly 505 etc ggc aaa Leu Sly Lye gee tge get Ale Cys Ala 510 1536 etc tta acc gao gga cgo ttc Len Leu Thr Asp Sly Arg Phe 515 gge gge aca tea ggt ttg te ate Sly Sly Thr Sr Sly Leu Ser Ile 525 1584 WO 01/85772 PCT/GB01/02003 gga cac gee tcg ccc gaa gcg gcg gaa ggt ggc gcg ate ggt ttg gta 1632 Gly His Ala Ser Pro Glu Ala Ala Glu Gly Gly Ala Ile Gly Leu Val 530 535 540 cae gaa gge gat ace gtc gaa ate gac ate ccc ate ge acge ate cge 1680 His GIu Gly Asp Thr Val. Glu Ile Asp Ile Pro Asn Arg Set Ile Arg 545 550 555 560 ctt gtc att tee gac gaa gaa etc gee gcc cgc cgt gee gaa atg gaa 1728 Leu Val Ile Ser Asp Glu GIu Lou Ala Ala Arg Arg Ala Glu Met Glu 565 570 575 gca cgc ggc age aaa gcg tgg aaa ccg gaa aac cgc gac cgc tac gtc 1776 Ala Arg Gly Ser Lys Ala Trp Lys Pro Glu Asn Arg Asp Arg Tyr Val 580 585 590 tee gee gca tta aga get tac gge gcg atg gcg act tee gee gac aaa 1824 cSer Ala Ala Leu Arg Ala Tyr Gly Ala Met Ala Thr Sex Ala Asp Lys 595 600 605 ggc gcg gtg cge gac gta gcg caa atc gaa aga 1857 Gly Ala Val Arg Asp Val Ala Gin Ile Glu Arg 610 615 <210> 28 <211> 619 <212> PRT <213> Neisseria meningitidis <400> 28 Met Pro Glu Tyr Arg Set Lys Thr Set Thr His Gly Arg Asn Met Ala 1 5 10 Gly Ala Arg Ala Leu Trp Arg Ala Thr Gly Val Met Glu Thr Asp Phe 25 Gly Lys Pro Ile Ile Ala Val Ala Asn Sex Phe Thr Gln Phe Val Pro 40 Gly His Val His Leu His Asn Met Gly Gln Leu Val Ala Arg Glu Ile 55 Lys Ala Gly Ala Ile Ala Lys Glu Phe Asn Thr Ile Ala Ile Asp 70 75 Asp Gly Ile Ala Met Gly His Ser Gly Met Leu Tyr Ser Leu Pro Ser 90 51 WO 01/85772 Arg Asp Leu Ala Asp Ala 115 Met Leu Ile 130 Gly Gly Pro 145 Pro Glu Arg 3 Asp Asn Ile S 1 Pro Thr Cys G 195 Cys Leu Thr.G 210 Leu Ala Thr Hi 225 Met Ile Val G1 Val Leu Pro Ar 26 Thr Met Asp Il 275 Leu Ala Val Alz 290 Asp Arg Leu Sex 305 Asn His Asp Tyr Ala Ile Leu Lys 340 I *1 L MI a A e 8 Lu :g 0 e a Ile Ala Asp Set Ile Glu LOO 105 5eu Val cys Ile Ser Asn 120 la Ala Met Arg Leu Asn 135 et Glu Ala Gly Lys Val 150 rg Leu Asp Leu Ile Asp 165 tr Asn Arg Gln Val Glu 10
16. 185- y Set Cys Set Gly Met p 200 u Ala Leu Gly Leu Ser L 215 s Val Gly Arg Lys Glu L 230 a Ile Thr Lys Arg Tyr T 245 2 Ser Ile Ala Thr Lys L5 265 Ala Met Gly Gly Ser Th 280 Ash Glu Ala Gly Val As 295 Arg Val Val Pro Cys 11I 310 Tyr Met Glu Asp Val His 325 33C Glu Leu Asp Lys Ala Gly 345 52 Tyr Met Cys Asp I Ile Pro T 1 Ile Gly V 155 Ala Met I: 170 ;lu Val GI he Thr Al eu Pro G1 22 eu Phe Le 235 yr Glu GIn 50 s Ala Phe r Asn Thr p Phe Lys 300 e Cys Lys 315 s Arg Ala Lys Leu PCT/GB01/02003 Val Asn Ala His Cys 110 zys Ile Thr Pro Gly 125 'hr Ile Phe Val Set al Ala Asn Ile Gin 160 le Glu Ser Ala Asp 175 Lu Gln Asn Ala Cys 190 .a Asn Ser Met Asn 205 y Asn Gly Set Tyr u Glu Ala Gly Arg 240 Asn Asp Glu Thr 255 Glu Asn Ala Met 270 Ile Leu His Leu 285 Met Ala Asp Ile Thr Ala Pro Asn 320 Gly Gly Ile Phe 335 His Thr Asp Val 350 WO 01/85772 His Thr lie His 355 Val Thr Asn Pro 370 Pro Gly Gly Val 385 Lys Thr Leu Asp His Ala Tyr Ser 420 Ala Glu Arg Gly 435 Leu Lys Phe Thr G 450 Val Glu Gly Ile L 465 Ile Arg Tyr Glu G: 41 Tyr Pro Thr Ser TJ 500 Leu Leu Thr Asp Gl 515 Gly His Ala Ser Pr 530 His Glu Gly Asp Th: 545 Leu Val Ile Ser As: 565 Ala Arg Gly Ser Lys 580 Ser Ala Ala Leu Arg 595 Ala Pro Thr Leu Lys 360 Glu Asn Thr His Ala 375 Arg Thr Thr Gin Ala 390 Leu As A Arg Glu Lys 405 Gln Asp Gly Gly Leu 425 Cys Val Val Lys Thr I 440 ly Arg Ala Arg Val P 455 eu Gly Asn Gin Ile V 470 ly Pro Lys Gly Gly P: 35 4 yr Leu Lys Ser Lys GJ 505 y Arg Phe Ser Gly GI 520 SGlu Ala Al Aa Glu GI .535 r Val Glu Ile Asp Il 550 p Glu Glu Leu Ala Ala 5 570 Ala Trp Lys Pro Glu 585 Ala Tyr Gly Ala Met 600 53 Asp Ala Ile Glu Phe Ser 395 Gly Cys 410 Ala Val L la Gly V 'he Glu S 4( al Ala GJ 475 ro Gly Me 90 4y Leu Gl y Thr Se y Gly Ala 54C e Pro Asn 555 Arg Arg Asn Arg Ala Thr PCT/GB01/02003 Ile Glu Lys Trp Asp 365 Arg Phe Lys Ala Ala 380 Gln Asn Arg Met Trp 400 Ile Arg Asp Val Ala 415 eu Phe Gly Asn Ile 430 al Asp Glu Ser Ile 445 er Gin Glu Asp Ala Ly Asp Ile Val Ile 480 t Gin Glu Met Leu 495 y Lys Ala Cys Ala 510 r Gly Leu Ser Ile 525 Ile Gly Leu Val Arg Set Ile Arg 560 Ala Glu Met Glu 575 Asp Arg Tyr Val 590 Ser Ala Asp Lys 605 WO 01/85772 PCT/GBo1/ozoo3 Gly Ala Val Arg Asp Val Ala Gin le Glu Arg 610 615 <210> 29 <211> 1725 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1725) <400> 29 atg eag eta. tea gge geg eaa ate ata gtg cag agt etc aaa gee gsa 48 Met Girt Leu 8cr Gly Ala Gin Ile Ile Val Gin Set Leu Lys Ala Giu 1 5 10 ggt gtc gag tac gtt ttc ggt tat ccc gge ggt geg gtt etc gaa ate 96 GlY Val. Giu Tyr Val Phe Gly Tyr Pro (fly Gly Ala Val Ie Giu Ile 25 tae gat gee ctt tte caa etc ast aes ttc sag cac att etg ace cgt 144 Tyr Asp Ala Leu Phe Gin Leu Asn Lys Phe Lys His Ile Leu Thr Arg 40 cac gag cag geg gee gta cac geg gea gat geg tat gcg ego gte age 192 His Gin Gin Ala Ala Val His Ala Ala Asp Ala Tyr Ala Arg Val Set 55 ggt sag gtg gge gtg gea ttg gtt aes tee gge ccg ggc gtt ace sat 240 Gly Lys Val Gly Val Ala Len Val Thr Ser Gly Pro Gly Val Thr Asn 70 75 gee etg ace ggt att get act gee tat aeg gat teg att ceg atg gtg 288 Ala Len Thr Gly Ile Ala Thr Ala Tyr Thr Asp 8cr Ile Pro Met Val. 90 gtc ate age ggg cag gta gge sat tee ctg att ggt aeg get geg tte 336 Val Ile Ser Sly Gin Val Gly Asn 5cr Len Ile Gly Thr Asp Ala Phe 100 105 110 eaa gas gtt gat aeg gtg ggt att ace cgt ccg tge gte ac cac eat 384 Gin Giu Vai Asp Tbr Val Gly Ile Thr Arg Pro Cys Val Lys His Asn 115 120 125 54 WO 01/85772 PCT/GBOI/o2003 ttc ctg gtt aeg gac atc aat gag ttg geg gaa ace att aaa sag gag 432 Phe Leu Val Thr Asp Ile Aen GIu Leu Ala Glu Thr Ile Lys Lye Ala ISO ttc caa Phe Gin 145 att gee gca ago ggo ega Ile Ala Ala Sex Gly Arg 150 cog ggg ccc gtg gtg Pro Gly Pro Val Val 155 gcg aa ttc age tat Ala Lye Phe Set Tyr 170 ccg aaa gat gtt Pro Lys Asp Val gtt gat gte Vai Asp Val 160 cct cag gas Pro Gin Glu 175 acg cay geg &tg Thr Gin Ala Met 165 gac att ttt ASp Ile Phe eg att aaa Gin Ile Lye 195 atc cgt lie Arg 180 tcg tat caa cog gtt gtt can ggg Ser Tyr Gin Pro Vai Val Gin Gly 185 cat ate ggt His Ile Gly 190 agt cog gte Arg Pro Val aag gee gtg cag Lye Ala Val Gin ttg gca tot gee Len Ala Sr Ala gte tat Val Tyr 210 ace ega Thr Arg 225 ttt gge gge gge gtg gtg ttg ggt sat Phe Giy Gly Gly Vaal Val Leu Gly Asn tct gaa gag etg Ser GiU Gin Len ttt gte ega atg aeg ggt got cg tge aeg ggt aeg etg atg Phe Val Ary Met Thr Giy Ala Pro Cys Thr Sly Thr Len Met gge ttg gye got tat cct tee gge gac Gly Leu Gly Ala Tyr Pro Ser Gly Asp 245 caa tte etc ggt Gin Phe Len Gly atg etc Met Len 255 ggt aty cac Gly Met His ggt Sly 260 act tac gag gca Thr Tyr Glu Ala ett gee atg cag Len Ala Met Gin aat gag gat Asn Ala Asp 270 gtt tee gta Val Ser Val gte gtt ott gee Va1 Vai Leu Ala 275 gta ggt gag Val Gly Ala ttt gac gac gt Phe ASp Asp Arg ccg tee Pro Ser 290 aaa ttt ttc gag sag gag aag aag gtt Lyse Phe PM Gln Lye Ala Lye Lys Val eat atc gat gte His Ile Asp Val gat Oct tee age ate gee aaa C9e gtg aag gag gac att ccg att gte Asp Pro Ser Ser Ile Ala Lys Arg Vai Lye Ala Asp Ile Pro Ile Val 305 310 315 320 WO 01/85772 PCT/GBOI/o200J ggc gao gtg aaa Sly Asp Val Lys aao gtt ttg too gag atg gtt gog otg tgg osa aaa Asn Val Leu 8cr Glu Met Val Ala Lou Trp Gin Lys 325 330 335 1008 caa gag too G;In Glu 5cr cog tat gau gut got ttg ggo aaa tgg Pro Ser Giu Asp A-la Leu Sly Lys Trp tgg aaa aco Trp Lys Thr 350 auc ggc ago Asn Sly Ser ata gag gua tgg ogt too ego Ile Giu Giu Trp Arg Sex Arg tgo ttg tgg ttt Cys Leu Trp Phe 1056 1104 1152 1200 gaa att Glu Ile 370 ggo aut Sly An 385 ate aug oca cuu Ile Lys Pro Gin gtg utt cug aug Val Ile Gin Lye gcc gag att ace Ala (flu Ie Thr tog gou ato Ser Ala Ile uca tog gut gtu Thr 5cr Asp Val ag oat oaa atg Gin His Gin Met gog got oua tat Ala Ala Gin Tyr tat Tyr 405 coo tto gaa ogt Cog ago ona tgg oto auc too Pro Phe Glu Arg Pro Arg Gin Trp Leu Asn 5cr 1248 ggo ggt ttg Sly Sly Leu aaa ott gc Lys Lou Ala 435 ggt Gly 420 aog utg ggo gtt Thr Met Gly Val otg cot tat gog utt ggt goa Leu Pro Tyr Ala Ile Sly Ala 430 1296 goo cog gut oau Ala Pro Asp Gin gta ttc tgt att Val. Phe Cys Ile ggc gao ggo Sly Asp Gly 1344 tog uto Sex Ile 450 cag atg auc ato Gin Met fu Ile gag ctg too ace Giu Leu 8cr Thr tto ouu tat cgg Phe Gin Tyr Arg 1392 1440 cog gtt aac gto Pro Val Asn Val acg otg auc aao Thr Leu Asn An tat oto ggt Tyr Len Sly atgr gta met Val ogo oug tgg oug gnu ata tat tao ggo ggu oga gag tog gaa aoo tat Arg Gin Trp Gin Gi Ile Tyr Tyr Sly Sly Arg Gin 5cr Giu Thr Tyr 485 490 495 tto gut tot ttg coo gat tto gto uaa ott goo gag goa tao ggo cat Phe Asp Ser Leu Pro Asp Phe Val Lys Len Ala Gin Ala Tyr Gly His 500 505 510 1488 1536 WO 01/85772 PCT/GB01/02003 ate ggt atc cgc gtg gac aag aag tct gat gtg gaa ggt gcg ttg ttg 1584 lie Gly Ile Arg Val Asp Lys Lys Set Asp Val Glu Gly Ala Leu Leu 515 520 525 gaa gca ttg aac caa aaa gac agg ctg gtg ttt atc gac ttc ctg acc 1632 Glu Ala Leu Asn Gin Lye Asp Arg Leu Val Phe Ile Asp Phe Leu Thr gac cag aaa cag aat gtg atg ccc atg gtc ggc aac gge aaa ggt ttg 1680 Asp Gin Lys Gln Asn Val Met Pro Met Val Gly Ash Gly Lys Gly Leu 545 550 555 560 gac gaa atg gta ctt ccg ccg cat atg cgt gcg gac gga aag gcg 1725 Asp Glu Met Val Leu Pro Pro His Met Arg Ala Asp Gly Lys Ala 565 570 575 <210> <211> 575 <212> PRT <213> Neisseria meningitidis <400> Met Gin Leu Set Gly Ala Gin Ile lle Val Gin Ser Leu Lys Ala Glu 1 5 10 Gly Val Glu Tyr Val Phe Gly Tyr Pro Gly Gly Ala Val Ile Glu Ile 25 Tyr Asp Ala Leu Phe Gln Leu Asn Lys Phe Lys His Ile Leu Thr Arg 40 His Glu Gin Ala Ala Val His Ala Ala Asp Ala Tyr Ala Arg Val Ser 55 Gly Lys Val Gly Val Ala Leu Val Thr Set Gly Pro Gly Val Thr Ann 70 75 Ala Leu Thr Gly Ile Ala Thr Ala Tyr Thr Asp Set Ile Pro Met Val 90 Val Ile Ser Gly Gin Val Gly Ann Set Leu Ile Gly Thr Asp Ala Phe 100 105 110 Gln Glu Val Asp Thr Val Gly lle Thr Arg Pro Cys Val Lys His Asn 115 120 125 Phe Leu Val Thr Asp Ile Asn Glu Leu Ala Glu Thr Ile Lys Lys Ala 57 WO 01/85772 130 Phe Gin Ile Ala 145 Pro Lys Asp Val Asp lie Phe Ile 180 Gin Ile Lys Lys 195 Val Tyr Phe Gly G 210 Thr Arg Phe Val A 225 Gly Leu Gly Ala T 3 24 Gly Met His Gly Th 260 Val Val Leu Ala Va 275 Pro Ser Lye Phe Ph 290 Asp Pro Ser Ser Ile 305 Gly Asp Val Lys Asn 325 Gin Glu Ser Val Pro 340 Ile Glu Glu Trp Arg 355 Glu Ile Ile Lys Pro 370 Gly Asn Ser Ala Ile PCT/GB01/02003 Ala Ser Gly Arg Pro Gly 150 Thr Gin Ala Met Ala Lys 165 170 Arg Ser Tyr Gin Pro Val 185 la Val Gin Met Leu Ala 200 ly Gly Val Val Leu Gly I 215 rg Met Thr Gly Ala Pro C 230 2 rr Pro Set Gly Asp Arg G 15 250 r Tyr Glu Ala Asn Leu A: 265 1 Gly Ala Arg Phe Asp As 280 e Glu Lys Ala Lys Lys Va 295 SAla Lys Arg Val Lys Al 310 31 Val Leu Ser Glu Met Val 330 Ser Glu Asp Ala Leu Gly 345 Ser Arg Asp Cys Leu Trp 360 Gin Tyr Val Ile Gin Lys 375 Ile Thr Ser Asp Val Gly 58 f s 1 I I a F L r Pro Val Val Val Asp Val 155 160 Phe Ser Tyr Pro Gin Glu 175 ial Gin Gly His Ile Gly 190 !er Ala Lys Arg Pro Val 205 an Ala Ser Glu Glu Leu 220 ys Thr Gly Thr Leu Met 35 240 Ln Phe Leu Gly Met Leu 255 *a Met Gln Asn Ala Asp 270 p Arg Val Val Ser Val 285 1 Ile His Ile Asp Val 300 Asp Ile Pro Ile Val 5 320 Ala Leu Trp Gln Lys 335 Lys Trp Trp Lys Thr 350 Phe Asp Asn Gly Ser 365 Leu Ala Glu Ile Thr 380 Gln His Gln Met Phe 31 Al Gl Ly Se Il< 46 Arg Phe Ile Glu Asp 545 Asp WO 01/85772 5 390 395 La Ala Gin Tyr Tyr Pro Phe Glu Arg Pro Arg Gin Trp 405 410 .y Gly Leu Gly Thr Met Gly Val Gly Leu Pro Tyr Ala 420 425 s Leu Ala Ala Pro Asp Gln Asp Val Phe Cys Ile Thr 435 440 445 r Ile Gin Met Asn Ile Gin Glu Leu Ser Thr Cys Phe 450 455 460 e Pro val Asn Val Ile Thr Leu Asn Asn Gly Tyr Leu 5 470 475 Gln Trp Gln Glu lie Tyr Tyr Gly Gly Arg Glu Ser C 485 490 Asp ser Leu Pro Asp Phe Val Lys Leu Ala Glu Ala T 500 505 5 Gly I1e Arg Val Asp Lys Lys Ser Asp Val Glu Gly A 515 520 525 Ala Leu Asn Gln Lys Asp Arg Leu Val Phe Ile Asp PI 530 535 540 Gin Lys Gln Asn Val Met Pro Met Val Gly Asn Gly L3 550 555 Glu Met Val Leu Pro Pro His Met Arg Ala Asp Gly Ly 565 570 PCT/GB01/02003 400 Leu Asn Set 415 Ile Gly Ala 430 Gly Asp Gly Gin Tyr Arg Gly Met Val 480 ;lu Thr Tyr 495 'yr Gly His la Leu Leu he Leu Thr is Gly Leu 560 's Ala 575 <210> 31 <211> 651 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 31 atg ttt tcc gta ccg cgt tec ttt ttg ccg ggc gtt ttc gta ctt gcc 48 59 WO 01/85772 Met Phe 3cr Val Pro Arg Bar Phe Lou Pro 1 5 10 gog ott goc goc tgc aaa cot oaa gao aao Ala Lou Ala Ala Cys Lys Pro Gin Asp An 25 tot toe agt gca too gog tog got gog gaa 8cr Sc: Set Ala 3cr Mla 5cr Mla Ala (flu 40 acg ogo ggt aog gat atg ogt aag gaa gao. Thr Arg Gly Thr Asp Met Arg Lys (flu Asp 55 otg aco gao ggo gaa ggo aag cot tto aac Lou Tb: Asp Gly Glu Gly Lys Pro Phe An 70 aag gto gtg att otg tot tto ggo ttt aog c Lye Val Val Ile Lou Ser Phe Gly Phe Thr H 90 cog aca gag ott ttg acg tao ago gao aog t Pro Tb: Giu Lou Lou Tb: Tyr Set Asp Tb: L 100 105 cag got aag gao gtg aaa gtg gtg tto gto aw Gli Ala Lys Asp Val Lys Val Val Phe Val S~ 115 120 gao aog oct gaa etc etc ggo aag tat goc aa Asp Tb: Pro Giu Ile Ile Gly Lys Tyr Ala L3 130 135 ttt ato ggt otg aog goa acg ggo gao caa aa Phe Ile Gly Lou Tb: Ala Tbr Gly Asp Gin As 145 150 15 cag cae tao ogo gtg gtt tot goc aaa gto aa Gin Gin Tyr Arg Val Val So: Ala Lys Val Asi 165 170 gaa aec tat ttg gto gao cac tot too ggt goc Glu Ann Tyr Lou Val Asp His 5cr Se: Gly A1z 180 185 aac ggt gag gtt goc att ttc tog cot tao gga Gly Val Pec agt gog gog Se: Mla Mla eat gog goa Ann Ala Mla etc ggo ggo Ile Gly Giy :tg ago getI EieU 8cr Asp :ao tgt coo g is Cys Pro tg eag Oag t cu Lys Gin L jo eto get 0 Ile Asp p; 125 *ta Og tto aa *S Gin Pho As 140 o ctg cog gt n Lou Pro Va 5 t ccc aaa ga n Gin Lys Asj J tat Oto etc ITyr Lou lE 190 ago gag cog Val caa Gin eag Lys get ttg 'eu rat Lsp tg c euC ro0 t c n P. .0 at 1 Ii o ga p As 17 ga ga IPCT/GBol/o2003 Lou Ala gto got 96 Vai Ala cog ca 144 Pro Gin ttc aog 192 Pbs T r aaa ggo 240 Lye Gly gto tgo 288 al Cys 9gO ggg 336 fY Y Gly aa ogo 384 *iu Arg cg gao 432 ro Asp ;c ag 480 Lys 160 *0 ago 528 p~ Sc: o aaa 576 p Lys a aog 624 WO 01/85772 PCT/GB01/02003 Asn Gly Glu Val Ala Ile Phe Ser Pro Tyr Gly Ser Glu Pro Glu Thr 195 200 205 att get gcc gat gta agg ace ctg etc 651 Ile Ala Ala Asp Val Arg Thr Leu Leu 210 215 <210> 32 <211> 217 <212> PRT <213> Neisseria meningitidis <400> 32 Met Phe Ser Val Pro Arg Ser Phe Leu Pro Gly Val Phe Val Leu Ala 1 5 10 Ala Leu Ala Ala Cys Lys Pro Gln Asp Asn Ser Ala Ala Gin Val Ala 25 Ser ser Ser Ala Ser Ala Ser Ala Ala Glu Asn Ala Ala Lys Pro Gln 40 Thr Arg Gly Thr Asp Met Arg Lys Glu Asp Ile Gly Gly Asp Phe Thr 55 Leu Thr Asp Gly Glu Gly Lys Pro Phe Asn Leu Ser Asp Leu Lys Gly 70 75 Lys Val Val Ile Leu Ser Phe Gly Phe Thr His Cys Pro Asp Val Cys 90 Pro Thr Glu Leu Leu Thr Tyr Ser Asp Thr Leu Lys Gin Leu Gly Gly 100 105 110 Gin Ala Lys Asp Val Lys Val Val Phe Val Ser Ile Asp Pro Glu Arg 115 120 125 Asp Thr Pro Glu Ile Ile Gly Lys Tyr Ala Lys Gin Phe Asn Pro Asp 130 135 140 Phe Ile Gly Leu Thr Ala Thr Gly Asp Gin Asn Leu Pro Val Ile Lys 145 150 155 160 Gln Gin Tyr Arg Val Val Ser Ala Lys Val Asn Gln Lys Asp Asp Ser 165 170 175 Glu Asn Tyr Leu Val Asp His Ser Ser Gly Ala Tyr Leu Ile Asp Lys 61 WO 01/85772 PCTfGBOI/o2003 180 185 190 AsnX Gly Glu Val Ala Ile Pho Her Pro Tyr Gly Ser Glu Pro Glu Thr 195 200 205 Ile Ala Ala Asp Vol Arg Thr Lou Leu 210 215 <210> 33 <211> 330 <212> DNA <213> Neissoria moningititis <220> <221> CDs <222> <400> 33 ttg cog ctg ott tgt tgt ott tgg ttt tgg cog cct gog gog gtg oso 48 Lou Pro Lou Leu Cys Cys Lou Trp Phe Trp Gin Pro Ala Ala Vol Lye 1 5 10 sag cog ctg aag oto cog ctg ctg aag coo otg cog cog sag ctc cog 96 Lys Pro Lou Lys Lou Pro Leu Lou Lye His Lou Pro Pro Lys Lou Pro 25 Ots ctg sag 000 ctg cog Cog sag oto cog otg otg ag coo otg cog 144 Lou Lou Lys His Lou Pro Pro Lys Lou Pro Lou Lou Lys His Lou Pro 40 cog sag oto ctg cta ctg sag ceo otg cog cog sag oto ccg otg otg 192 Pro Lys Lou Lou Lou Lou Lys His Lou Pro Pro Lys Lou Pro Lou Lou 55 sag Ctg cog ota cog sag oso otg Cog otg aog ctg cog ota cog sag 240 Lys Lou Pro Lou Pro Lys His Leu Pro Lou Lys Lou Pro Lou Pro Lye 70 75 cac otg cog ctg aag oto otg otg cog sag otg coo sat sag cot ttt 288 His Leu Pro Lou Lys Lou Lou Lou Pro Lye Leu Gin Asn Lye His Pho 90 cog ctt gos ass sag cog got sog tto agt ato otg ott ttt 330 Pro Lou Ala Lys Lye Gin Asp Thr Phe Her Ilo Lou Lou Pho 100 105 110 WO 01/85772 <210> 34 <211> 110 <212> PRT <213> Neisseri, <400> 34 Leu Pro Leu Le 1 Lys Pro Leu Lys Leu Leu Lys His Pro Lys Leu Leu Lys Leu Pro Leu His Leu Pro Leu Pro Leu Ala Lys 100 PCT/GB01/02003 a meningitidis I Cys Cys Leu Trp 5 Leu Pro Leu Leu Leu Pro Pro Lys 40 Leu Leu Lys His 55 Pro Lys His Leu 70 Lys Leu Leu Leu I Lys Gin Asp Thr P 1 Phi Lys 25 Leu Leu Pro Pro he 05 e Trp 10 s His SPro Pro Leu Lys 90 Ser I Gli Leu Leu Pro Lys 75 leu le SPro Pro Leu Lys Leu Gln Leu L Al Prc Lys Leu Pro Asn eu a Ala Val Lys o Lys Leu Pro His Leu Pro Pro Leu Leu Leu Pro Lys Lys His Phe Phe 110 <210> <211> 2118 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(2118) <400> atg ttc gac aaa cac gtt sag acc ttc caa tac ggt aat cag acc gtt Met Phe Asp Lys His Val Lys Thr Phe Gln Tyr Gly Asn Gin Thr Val 1 5 10 act ttg gaa acc ggc gas att gcc cgc caa gcc gcc gct gcc gtt aaa Thr Leu Glu Thr Gly Glu Ile Ala Arg Gin Ala Ala Ala Ala Val Lys 25 WO 01/85772 PCTIGBoI/0200s gtc tot atg ggc gao aco gt; gtt ttg gtt gco gtt acc acc aac aaa Val So: Met Gly Asp Thr Val Val Leu Val Ala Val Thr Thr Asn Lys AA 144 gaa gtg aaa Glu Val Lye gaa ggc caa gao tto ttc ccc ctg aco gtc gat tat ttg Glu Gly Gin Asp Phe Phe Pro Leu Thr Val Asp Tyr Leu ogo act tao goc Ar; Thr Tyr Ala ggt aaa atc c0 Gly Lys lie Pro ggt ttc tto aaa Gly Phe Phe Lys gaa ggc aaa caa Gl Gly Lys Gin gaa aaa gaa atc ctg acc ago cgt ctg Glu Lys Glu Ile Lou Thr Ser Ar; Leu atc gac Ile Asp ogc cog att Arg Pro Ile ato gta gcg Ile Val Ala 115 ogt Ar; 100 cog ctg tto coo Pro Len Phe Pro ggt tto tac cac Gly Phe Tyr His gao atc caa Asp Ile Gln 110 tcc gac att Ser Asp Ile at; gtc gtg tcc Met Val Val Ser gat ccc gaa ato Asp Pro Glu Ile oct gca Pro Ala 130 at; ttg ggo gag Met Len Giy Ala gco gag Otg Ala Ala Leu gtg ttg Val Lou 140 ago ggo gta cog Ser Giy Val Pro gao ggt cc; att Ala Gly Pro Ile gcg goa cgo gt; Ala Ala Arg Val tat ata aac ggo Tyr Ile Asn Gly 432 480 528 576 tao ;tt ttg aac Tyr Val Lou Asn cc; act Pro Thr 165 aaa gc gaa Lys Ala Glu ttg gcg Leu Ala 170 aaa tog can Lys Ser Gln ttg gao Len Asp 175 ttg gtg gto Leu Val Val gco aaa ato Ala Lys le 195 ;gt act ton aaa gc gtg ttg atg Gly Thr Ser Lye Ala Val Lou Met 185 gtg gaa too gas Val Glu Ser Glu 190 ctg cc gaa gao Len Pro Glu Asp atg ctg ggc gcg gtg gtt tao ggo Met Lou Gly Ala Val Val Tyr Gly 205 cac gat Oaa atg cag ;tt goc ato ant gca ata sat gaa ttt gco gac His Asp Gin Met Gin Val Ala lie Msn Ala Ile Asn Giu Phe Ala Asp 210 1 1t WO 01/85772 PCTIGBO1/02003 gaa gtc aat ccg gaa ctt tgg gat tgg aaa gca Oct gaa ace aat gag Gin Val Asn Pro Gin Len Trp Asp Trp Lys Ala Pro Giu Thr Aan Gin 225 230 235 240 gaa ctg gtt~gce aa Gin Lou Val Ala Lys 245 gag tto ass atecegt Ala Phe Lys le Arg 260 get tgg agt geg gta Ala Trp Se: Ala Val 275 gtc egc ggg att Val Arg Gly Ile ggc gaa ace Gly Gin Tb: tct gcc ass 8cr Ala Lys att ass gaa Ile LYS Gin 255 ttg gac gas Lu Asp Gin CAa aaa caa geg cgt Gin LYS Gin Ala Arg 265 asa gaa gee ttg att see gas gas see gao acg Lys Gin Ala Lau Ile Tb: Gin Gin Tb: Asp Thr 280 285 ctg ges gco sac Lou Ala Ala Asn 290 gas ate asa Giu Ie Lys 295 ggc stt tte ass Gly Ie Pbs Lys ttg gas gee gat Lou Gin Ala Asp gte gte cgc age eas att ttg gac ggo eas cg egC ate gac Val Val APrg 5cr Gin le Lou Asp Gly Gin Pro Arg le A.sp 305 310 315 ggCcoge Gly Arg 320 ttg ecg Lou Pro gac ace ego ace Asp Th: Arg Thr egt ccg etg sac Arg Pro Len Asn eas ace gge gta Gin Tbr Gly Val 1008 ego acg c& Arg Thr His gce gts geg Ala Vai Ala 355 tee ges ttg ttt. acc cgt ggc gaa see esa gee ctt Se: Ala Leu Phe Tb: Arg Gly Gin Tb: Giln Ala Len 1056 act ttg ggt act Tb: Lou Gly Thr teg cge gao gag caa ate 8cr Arg ASP Gin Gin Ile 360 365 Ate gac gg Ie Asp Ala 1104 etg tee Lou Se: 370 ggc gsa tac Gly Gin Tyr ace gao cge Tb: Asp Arg 375 ttt atg ctg Ose tac sac ttt ceg Phe Met Len His Tyr Asn Phe Pro 1152 1200 tac tet ace gge Tyr Se: Tb: Giy gas gtg ggc ege stg ggc Gin Val Gly Arg Met Gly 390 395 gog cg ass cge Ala Pro LYS Arg gaa ate ggt ese ggt cgt ttg get ass egt geatgtgge gatt Inlte Gly His Giy Arg Len Ala Lys Arg Ala Leu Len Ala Val. Leu 405 410 415 1248 WO 01/85772 PCTIGBOI/o2003 cog aaa act gaa gat tto ago tao aco atg cgc gtg gta tca gas att Pro Lys Pro Glu Asp Phe 3cr Tyr Thr Met Arg Val Val 3cr Gin lie 420 425 430 1296 aca gas tao sac Thr Sin 8cr Asm. 435 gga tat too tat atg got too gta tga Sly 5cr 3cr 3cr Met Mla 8cr Val cys ggo ggo tgo Sly Sly Cys 1344 ttg ago LeU 3cr 450 otg Otg too gao ggc gtg cat ttg LeU Leu 3cr Ala Gly Val Pro Leu 455 aas ga Lys Ala 450 a gtt goc ggt His Val Mla Sly 1392 gog atg ggt ctg Als Met Sly Leu atg gas ggo ast Leu Glu Gly Aan ttt gca gtc atg Pile Ala Val Len 1440 gao att ttg gga gao ASP Ie Len Sly Asp 485 got ggt aag aco gas Ala Sly Thr Thr Gin 500 gaa gao ao ttg Giu AsP His Len gat atg gac ttt Asp Met Asp Pile aaa gtg Lys Val 1488 ggo gtt cc Sly Val Thr gag atg a stg gao ata ass ata Ala LeU Gin Met Asp Ile Lys Ile 505 510 1536 o-aa ggo att soc ass gag Gin Sly Ile Thr Lys Giu att atg Ile Met 520 oaa ata got ttg gag Gin Ile Ala Len Ala 525 cag gao aa Sin Ala Lys 1584 gas gag agt ,Gin Ala Arg 530 atg aa ato ttg gat cag atg asa gao gao gtt gag ggo Len His Ile Leu Asp Gin Met Lys Ala Msa Val Ala Gly 1632 aaa gag otg Gin Gin Len too ga 3cr Ala 550 oao gag oaa ago ttg tto ag atg aaa His Ala Pro Arg Len Pile Thr Met Lys 555 1680 aaa caa gao aa Asa Gin Asp Lys ago gaa gtt ato Arg Gin Val Ile aag ggo ggt gaa Lys Sly Sly Sin aco sto Thr Ilie 1728 ogt gag att aoa got gaa a ggt aag gsa ato sat Arg Ala Ile Thr Mla Sin Thr Sly Thr Gin Ile Asn 580 585 att gao gsa gao Ile Ala Gin Asp 1776 1824 ggt ag att aoc att gao gaa ac aot as gas gao gga gat gag gag Sly Thr Ile Thr Ile Ala Ala Thr Tin Sin Siu Ala Sly Asp Ala Mla WO 01/85772 PCT/GB01/02003 aaa aaa cgc atc gag cag. att act gcc.ga gtg..ga gtg ggc aaa gtg 1872 Lys Lys Arg Ile Giu Gln Ile Thr Ala Giu Val Giu Val Giy Lye Val 615 620 tac gas ggc act gtg gtg aaa atc ctc gac aac aat gtc ggc gcg att 1920 Tyr Glu Gly Thr Val Val Lys Ile Leu Asp Asn Asn Val Gly Ala Ile 625 630 635 640 gtc agt gtg atg ccg ggc aaa gac ggt ttg gta cac atc ag caa ato 1968 Val ser Val Met Pro Gly Lys Asp Gly Leu Val His Ile Set Gln Ile 645 650 655 goc cac gag cgc gta cgc aat gtc ggc gac tac ctg caa gtc ggt cag 2016 Ala His Glu Arg Val Arg Asn Val Gly Asp Tyr Leu Gln Val Gly Gin 660 665 670 gtg gtg aac gtg aaa gca ttg gaa gtg gac gac aga ggc cgt gtc cgt 2064 Val Va.l Asn Val Lys Ala Leu Glu Val Asp Asp Arg Gly Arg Val Arg 675 680 685 ctg tcc atc aaa gcc ctg ctg gac gcg cct gcc cgt gag gas sat gcc 2112 Leu Ser Ile Lye Ala Leu Leu Asp Ala Pro Ala Arg Glu Glu Asn Ala 690 695 700 gcc gag Ala Glu 2118 705 <210> 36 <211> 706 <212> PRT <213> Neisseria meningitidis <400> 36 Met Phe Asp Lys His Val Lys Thr Phe Gln Tyr Gly ASh Gln Thr Val 1 5 10 Thr LeU Glu Thr Gly Glu Ile Ala Arg Gln Ala Ala Ala Ala Val Lys 25 Val Ser Met Gly Asp Thr Val Val Leu Val Ala Val Thr Thr Aan Lys 40 Glu Val Lys GIu Gly Gln Asp Phe Phe Pro Leu Thr Val Asp Tyr Leu 55 Glu Arg Thr Tyr Ala Ala Gly Lys Ile Pro Gly Gly Phe Phe Lys Arg 67 WO 01/85772 PCT/GB01/02003 70 75 Olu Gly Lys Gln Ser Glu Lys Glu Ile Leu Thr Set Arg Leu Ile Asp 90 Arg Pro Ile Arg Pro Leu Phe Pro Glu Gly Phe Tyr His Asp Ile Gln 100 105 110 Ile Val Ala Met Val Val Ser Val Asp Pro Glu Ile Asp Ser Asp Ile 115 120 125 Pro Ala Met Leu Gly Ala Ser Ala Ala Leu Val Leu Ser Gly Val Pro 130 135 140 Phe Ala Gly Pro Ile Gly Ala Ala Arg Val Gly Tyr Ile Asn Gly Val 145 150 155 160 Tyr Val Leu Asn Pro Thr Lys Ala Glu Leu Ala Lys Ser Gin Leu Asp 165 170 175 Leu Val Val Ala Gly Thr Ser Lys Ala Val Leu Met Val Glu Ser Glu 180 185 190 Ala Lys Ile Leu Pro Glu Asp Val Met Leu Gly Ala Val Val Tyr Gly 195 200 205 His Asp Gin Met Gin Val Ala Ile Asn Ala Ile Asn Glu Phe Ala Asp 210 215 220 Giu Val Asn Pro Glu Leu Trp Asp Trp Lys Ala Pro Glu Thr Asn Glu 225 230 235 240 Glu Leu Val Ala Lys Val Arg Gly Ile Ala Gly Glu Thr Ile Lys Glu 245 250 255 Ala Phe Lys Ile Arg Gln Lys Gln Ala Arg Ser Ala Lys Leu Asp Glu 260 265 270 Ala Trp ser Ala Val Lys Glu Ala Leu Ile Thr Glu Glu Thr Asp Thr 275 280 285 Leu Ala Ala Asn Glu Ile Lys Gly Ile Phe Lys His Leu Glu Ala Asp 290 295 300 Val Val Arg Ser Gln Ile Leu Asp Gly Gin Pro Arg Ile Asp Gly Arg 305 310 315 320 Asp Thr Arg Thr Val Arg Pro Leu Asn Ile Gin Thr Gly Val Leu Pro 68 WO 01/85772 PCT/GB01/02003 325 330 335 Arg Thr His Gly Ser Ala Leu Phe Thr Arg Gly Glu Thr Gin Ala Leu 340 345 350 Ala Val Ala Thr Leu Gly Thr Ser Arg Asp Glu Gln Ile Ile Asp Ala 355 360 365 Leu Set Gly Glu Tyr Thr Asp Arg Phe Met Leu His Tyr Asn Phe Pro 370 375 380 Pro Tyr Ser Thr Gly Giu Val Gly Arg Met Gly Ala Pro Lys Arg Arg 385 390 395 400 Glu Ile Gly His Gly Arg Leu Ala Lys Arg Ala Leu Leu Ala Val Leu 405 410 415 Pro Lys Pro Glu Asp Phe Set Tyr Thr Met Arg Val Val Set Glu Ile 420 425 430 Thr Glu.Ser Asn Gly Ser Set Ser Met Ala Ser Val Cys Gly Gly Cys 435 440 445 Leu Set Leu Leu Ser Ala Gly Val Pro Leu Lys Ala His Val Ala Gly 450 455 460 Ile Ala Met Gly Leu Ile Leu Glu Gly Asn Lys Phe Ala Val Leu Thr 465 470 475 480 Asp Ile Leu Gly Asp Glu Asp His Leu Gly Asp Met Asp Phe Lys Val 485 490 495 Ala Gly Thr Thr Gu Gly Val Thr Ala Leu Gln Met Asp Ile Lys Ile 500 505 510 Gln Gly Ile Thr Lys Glu Ile Met Gin Ile Ala Leu Ala Gln Ala Lys 515 520 525 Glu Ala Arg Leu His Ile Leu Asp Gln Met Lys Ala Ala Val Ala Gly 530 535 540 Pro Gin Glu Leu Ser Ala His Ala Pro Arg Leu Phe Thr Met Lys Ile 545 550 555 560 Asn Gln Asp Lys Ile Arg Glu Val Ile Gly Lys Gly Gly Glu Thr Ile 565 570 575 Arg Ala Ile Thr Ala Gl Thr Gly Thr Glu Ile Asn Ile Ala Glu Asp 69 WO 01/85772 PCT/GB01/02003 580 585 590 Gly Thr Ile Thr Ile Ala Ala Thr Thr Gin Glu Ala Gly Asp Ala Ala 595 600 605 Lys Lys Arg Ile Glu Gln Ile Thr Ala Glu Val Glu Val Gly Ly Val 610 615 620 Tyr Glu Gly Thr Val Val Lys Ile Leu Asp Asn Asn Val Gly Ala Ile 625 630 635 640 Val Ser Val Met Pro Gly Lys Asp Gly Leu Val His Ile Ser Gln Ile 645 650 655 Ala His Glu Arg Val Arg Asn Val Gly Asp Tyr Leu Gln Val Gly Gln 660 665 670 Val Val Asn Val Lys Ala Leu Glu Val Asp Asp Arg Gly Arg Val Arg 675 680 685 Leu Se9 Ile Lys Ala Leu Leu Asp Ala Pro Ala Arg Glu Glu Asn Ala 690 695 700 Ala Glu 705 <210> 37 <211> 3972 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(3972) <400> 37 gtg age cga att atg tct gtc gtt ttg ccc ttg cge ggc gtt acc.gcc 48 Val Ser Arg Ile Met Ser Val Val Leu Pro Leu Arg Gly Val Phr Ala 1 5 10 ctt tcc gat ttc cgt gtt gaa aaa Ctc ttg caa aaa gcc gcc gca ctc 96 Leu Ser Asp Phe Arg Val Glu Lys Leu Leu Gln Lys Ala Ala Ala Leu 25 ggt ctg ccc gaa gtc aaa tta agc agc gaa ttt tgg tat ttc gtc ggc 144 Gly Leu Pro Glu Val Lys Leu Ser Ser Giu Phe Trp Tyr Phe Val Gly WO 01/85772 PCT/GEoI/02003 aga gag aaa gca ctt gat gao gag act gta gaa aaa ctg caa gcc ttg Sor Glu Lys Ala Leu Asp Ala Ala Thr Val Glu Lys Lou Gin Ala Leu m r ttg gcg gcg Lou Ala Ala aaa ago gtt gaa caa acg oca aaa gog ago gag ggc ttg Gin Set Val Glu Gin Thr Pro Lys Ala Arg Glu Guy Leu cat ttg ttt ttg His Lou Phe Lou acg ccc agt ttg Thr Pro Arg Lou acg att tag ccg Thr Ile Set Pro tgg got Trp Ala tc aag gag Set Lys Ala aat abc gag gaa aac tga ggt ttg gas ggo abc ga, Asn Ile Ala Giu Ass C's Guy Lou Ala Sly Ile Glu C9 abc gag cga ggc abg gag gtg bgg Ag Ile Glu Arg Sly Met Ala Val Trp 115 120 ctg gsa ggb gcg Lou Glu Sly Ala 125 ctt acc gst Leu Thr Asp gas cag Glu Gin 130 aaa cag caa tgg gag gat btg Gin Gin Gin Trp Ala Ala Lou 135 atg cac gac cga atg act gas Leu His Asp Arg Met Tbr Glu 140 gtg ctg cca gat Val Leu Pro Asp cag acg gca to Gin Thr Ala Ser aaa tta Lys Leu 155 tt cac cat Pho His His 480 gad ta gas acc Glu Ser GIu Thr ttb too Phe Ser 165 acc gta gab Thr Val Asp ttg ggc ggc Lou Guy Gly gab ttg gta Ala Lou Val gas ata gab Glu Ile Asp 195 gc ast ac0 gaa Mla Ass Thr Glu ggt ttg gca etc Sly Lou Ala Leu ggt aaa gag Sly Lys Glu 175 tea gao gao Set Ala Asp 190 cgc sat cog Arg Ass Pro 528 576 624 tat ctg gtt gas Tyr Lou Val Glu tat cag got btg Tyr Gin Al Leu tct gat gtg gag Sat Asp Val Glu 210 atg atg atg tto gag cag gca aac aga gas cac tga Lou Met Met Phe Ala Gin Ala Ass Set Glu His Cys agc cac aaa abc tta aac gao gao ttc abc etc aac ggc gas aaa cag Arg His Lys Ile Pho Asn Ala Asp Phe ie Lou Ass Guy Glu Lye Gin WO 01/85772 PCT/GBol/02003 240 Cog aaa tog otc ttc ggt atg ata cg gao aeg cac aao gog cat ccc Pro Lys ser Leu Phe Gay Met lie Arg Asp Tbr His An Ala His Pro 245 250 255 gaa ggo aca gto gtt gc tao aaa gao aao tca too gtg atc gaa ggc Glu Sly Thr Val Val Ala Tyr LyS Asp Ann Ser Ser Val Ile Giu Gly 260 265 270 768 816 gca aaa gtc Ala Lys Val 275 gas cgt ttc tat Glu Arg Phe Tyr aat gg ga gas As Ala Ala Gin oaa ggc tac Gin Sly Tyr cgt ttc Arg Phe 290 cac gag gaa gac His Glu Giu Asp CC aco goc atc Pro Thr Ala Ile 310 cat atc ate atg His Ile Ile Met gtg gaa acg cac Val Giu Thr His cac His gg cog ttt gcg Ala Pro Phe Ala gcg gca acg ggc Ala Ala Thr Sly ggo ggc gaa Gly Sly Glu atc cgc Ile Arg 325 gac gaa ggt gog acg ggo aaa ggt tog ogt ccg AsP Glu Gly Ala Thr Gly Lys Sly 3cr Arg pro 330 1008 aaa gca ggc Lys Ala Gly ttg aco Leu Thr 340 ggo ttt soc Gly Phe Thr gtg too Val Ber 345 sac ctc aac Ann Leu Asn atc coo ggo Ile Pro Sly 350 1056 otc aaa Leu Lye tcg ccg Bar Pro 370 sac sac Asn Asn 385 cag cog tgg gaa cas gat tao ggc Gin Pro Trp GiU Gin Asp Tyr Gly 355 sag cog gaa oat att too Lys Pro Glu His Ile Ber 365 ctg gao att atg LeU Asp Ile Met att gas Ile Glu 375 ggc ccc ato ggc ggo gog gcg ttc Sly Pro Ile Sly Sly Ala Ala Phe gaa tto ggc cgo ccc sac otc ttg Au Phe Giy Arg Pro Asn Leu Leu tao ttc cgo act Tyr Phe Arg Thr 1104 1152 1200 1248 1296 gaa gaa aaa ttt Glu GiU Lys Phe gao ggt cag gtt ogc ggo tac cac aaa cog att atg Asp Sly Gin Val Arg Sly Tyr His Lys Pro Ile Met 405 410 415 att gc ggo ggc ttg ggo ago att cag gog cag cag acg cat aaa gao Ile Ala Sly Sly Leu Gly Sor Ile Gin Ala Gin Gin Thr His Lys Asp WO 01/85772 PCTIGBOI/o2003 gaa ato ccc gaa ggc gog ttg otg ato (flu Ile Pro Giu Gly Ala Leu Leu Ile 435 440 caa ctg ggc ggc cog ggt atg Gin Leu Gly Gly Pro Gly Met 445 1344 ott abc Lou Ile 450 ggc ttg ggc ggc Gly Lou Gly Gly g gog got tot Gly Ala Ala Set 455 ttc aac too gta Phe Asn Se: Val tog atg gat Sor Met Asp 460 aco ggo aca Th: Gly Thr 1392 sac gao gog tot tta gao Asn Asp Ala Ser Leu Asp 465 470 ogo ggc eac Occ gas Arg Sly Asn Pro Giu 480 1440 ato gas cgo cgo Ile Giu Arg Arg qg cag gaa gbg etc Ala Gin Glu Val Ile 485 a go tgc tgg ca; cto ggc Arg Cys Trp Gin Leu Gly 495 1488 gao a aac Asp Lys Asn att ato too etc Ile Ile 3cr Ile gao gtt ggo gag Asp Val Gly Ala ggC ggo tbg Gly Gly Leu 510 ggc gog gta Gly Ala Val 1536 tcc aac gco ttc ccc gas otc gtc aftc gat gco 3cr Asn Ala Phe Pro Giu Leu Val Asn Asp Ala gga ogo Gly Arg 525 1584 ttC aeg ctg ogo Phe LYS Lou Arg 530 gaa gtg cog Glu Val pro 535 ctg gea gaa ceo Leu Glu Glu His abc aao cog ctg Leu Asn Pro Lou 1632 caa abc tgg tgc sac gaa Gin Ile Trp Cys Asn Giu tcg ces gag ogt tat gte tbg tog att 3cr Gin Glu Ar; Tyr Val Leu 3cr Ile 555 1680 gaa aaa gat ttg GIU LYS Asp Leu ato tto ogo tcc Ile Phe Arg Se: bgo gee cgt gaa Cys Giu Ar; Glu cgc bgo Arg Cys 575 1728 cog ttt go; Pro Phe Ala ogo gao gab Ar; Asp Asp 595 gto ggt ac; gog Val G1y Tb: Ala gao gao ggo cat Asp Asp Gly His ttg aea gta Leu Lys Val 1776 ttg tto too ao aso Cct gta get btg cog ttg Sac gto Leu Phe 3cr Asn Asn Pro Val ASP Lou Pro Leu Arn Val 1824 1872 btg oto ggc a cog ccc ass aco acg ogt aoc gao aaa acg gtt gag Leu Leu Gly Lys Pro Pro Lys Th: Thr Arg Th: Asp Lys Thr Vai Ala WO 01/85772 PCT/GB01/02003 CCg tcc aaa aaa ccg ttt cac gcg ggc gat atc gac att act gaa gee Pro Ser Lys Lys Pro Phe His Ala Gly Asp Ile Asp Ile Thr Glu Ala 625 630 635 640 1920 gcc tac cgc Ala Tyr Arg gtt ctg cge ctg cct Val Leu Arg Leu Pro 645 ge gta gcc Ala Val Ala 650 gec aaa aac ttc ctg Ala Lys Asn Phe Leu 655 1968 att ace atc Ile Thr Ile ggc gac cgc age gtc ggc ggc atg acc cac age gac caa Gly Asp Arg Set Val Gly Gly Met Thr His Arg Asp Gln 660 665 670 2016 atg gtc Met Val atg ggc Met Gly 690 ccg ace Pro Thr 705 aaa tac caa ace ccc gta gcc gac Lys Tyr Gln Thr Pro Val Ala Asp tgc gcc Cys Ala 685 gtt ace atg Val Thr Met ggc gaa aaa Gly GIu Lys 2064 ttc aac act tat Phe Asn Thr Tyr ggc gaa gcg atg Gly Glu Ala Met agc atg Ser Met 700 2112 gtc gec ctg Val Ala Leu gac gcg act get tcg gge aga atg tgc Asp Ala Pro Ala Set Gly Arg Met Cys 2160 ggc gaa gcc ate Gly Glu Ala Ile aac atc gcg gca gec aat atc ggc gac Ash Ile Ala Ala Ala Asn Ile Gly Asp ate ggc Ile Gly 735 2208 aac atc aaa Asn Ile Lys gaa gac gaa Glu Asp Glu 755 tcc gee aac tgg Sex Ala Asn Trp gcg gag tgc gge Ala Ala Cys Gly aac gaa ggc Asn Glu Gly 750 aaa gee tgt Lys Ala Cys 2256 2304 aaa ctc tac cge Lys Leu Tyr Arg gt gaa gcg gtt Val Gu Ala Val cag gca Gin Ala 770 ttg gat ttg Leu Asp Leu age att Ser Ile 775 ccc gtg ggc aaa. Pro Val Gly Lys agc ctg tcg atg Set Leu Set Met 2352 2400 ace gtg tgg Thr Val Trp cag gac ggc gaa gag aaa Gln Asp Gly Glu Glu Lys 790 aaa tee gtc gtt tcg ccg Lys Set Val Val Ser Pro 795 800 ttg age ctg att ate tce gcg ttc gag ccg gtt caa gac gta cge aaa Leu Ser Leu Ile Ile Set Ala Phe Ala Pro Val Gln Asp Val Arg Lys 2448 WO 01/85772 PCT/Gnol/020 03 ace gtt aeg ccc gee ttg ao aac gte gag gac age gta ttg etg ttt Thr yal Thr pro Giu Leu Lys Asn Val Glu Asp 3cr Val Leu Lou Phe 820 825 830 2496 gte get ttg Val Asp Leu 835 gge ttc gge aaa geg cgt atg ggc ggc Gly Phe Gly Lys Ala Arg Met Gly Gly 840 tg gg ttt ggt Sex Ale Phe Gly 845 ttg gee gat aca Leu ASP ASP Thr 2544 2592 ecag gtg Gin Val 850 :agt cgt 3cr Arg 865 tec aec eat Tyr Asn Ann, atg age ggc gac geg ccc get Met 3cr Gly Asp Ala pro Asp 855 860 ctg aao geg Len Lys Ala ttt tac aac gtg att cog Cog ott gte Phe Tyr Ann Val Ile Gin Gin Leu Val 870 875 tat eec gac cgc age gee ggc gge ttg Tyr His Asp Axrg 3cr Asp Gly Gly Len 890 gee aaa ctc ASP LYS Lou ttg geg Len Ala 885 gee ga Ala Gin 880 ttt gee Phe Ala 2640 2688 gtt ttg gta 'Val. Len Vai tta oat. tta Len Asn Leu 915 tet eac tca SeX Gin Ser 930 gaa atg Glu Met 900 gcg ttt gee Ala Phc Ala ggg egg tgc ggc ttg G~ly Arg Cys Gly Len 905 get ate get Asp Ile Asp 910 ace gct ctg Thr Ala Lou ttg ctt gee caa Len Leu Ala Gin ttt att cc Pe Ile Thr aec cat Ann His 2736 2784 2832 ttg cgg act Len Arg Tin gag gte aaa gcg Gin Val Lys Ala ttg gt gaa tgg caa Leu Ala Giu Trp Gin eec att gee Thr Ile Ala ege aca Arg Tin 950 tta ttt eat gee Len Phc Ann Gin gag ttg Gin Len ggt get gtt Gly Ala Val 2880 ce gtt aga aa Gin Yea A-rg Ly, caa gat gtt Gin Asp Val 965 gee gat att Ala Asp Ile 970 ate aet tta ttc tat ca Ile Asn Len Phe Tyr Gin 2928 ec ceg etg Gin Gin Len cat eat gte ttt His Ann Yal Ph gee. etc ggt aeg tta act get gag Gin Ile Giy Thr Leu Thr Asp Gin 985 990 2976 aec aeg tta ate etc ege gee ggg eea aeg eec ctt att tet gee eec Asn Thr Len Ile Ile Axg Asp Gly Gin Thr His Len Ile 3cr Asp Asn 3024 WO 01/85772 PCT/GBOI/02003 1000 1005 cta atec aaa ctg caa caa acc tgg caa gaa acc age cat caa atc caa Leu Ile Lys Leu Gln Gln Thr Trp Gin Glu Thr Ser His Gln Ile Gin 1010 1015 1020 3072 cgC ctg cgc Arg Leu Arg 1025 gac aac cct gcc tge gcc gac agc gag ttt Asp Asn Pro Ala cys Ala Asp Ser Glu Phe 1030 1035 gcc ctg att Ala Leu Ile 1040 3120 gge gac aac gga cge agc gca Gly Asp Asn Gly Arg Set Ala 1045 ttg ttt gee aac ctg aaa ttc gac gtg Leu Phe Ala Asn Leu Lys Phe Asp Val 1050 1055 3168 3216 aac gaa gac ate gcc gcg cog ttt atc aac age Asn Glu Asp Ile Ala Ala Pro Phe Ile Asn Ser 1060 1065 ate gee atec ctg cgc Ile Ala Ile Leu Arg 1075 gge gcg aaa ccc aaa Gly Ala Lys Pro Lye 1070 caa atc gaa atg gcc Gin Ile Glu Met Ala 1085 gaa cag gge gta aac ggg GiU Gin Gly Val Asn Gly 1080 3264 gee gCee tte ace cgt gee ggt ttc gat gee tac gac gtg cat atg tce Ala Ala Phe Thr Arg Ala Gly Phe Asp Ala Tyr Asp Val His Met Ser 1090 1095 1100 3312 gac ctg atg gca gge cgc gte Asp Leu Met Ala Gly Arg Val 1105 1110 gcg tgc gge gge ttc agc tao Ala Cys Gly Gly Phe Set Tyr 1125 cac ctt gee gac ttc aaa atg ctg gcg His Leu Ala Asp Phe Lys Met Leu Ala 1115 1120 ggc gac gta etc ggc gcg gge aaa ggc Gly Asp Val Leu Gly Ala Gly Lys Gly 1130 1135 3360 3408 tgg gcg aaa tcc atc ctg Trp Ala Lys Ser Ile Leu 1140 ttc cac ccc gee tta cgc gac cag tte gee Phe His Pro Ala Leu Arg Asp Gln Phe Ala 1145 1150 3456 gee ttc ttc ace gac Ala Phe Phe Thr Asp 1155 ccg aac acg ctg aca Pro ASn Thr Leu Thr 1160 ttg gge gtg tgc aac ggc Leu Gly Val Cys Asn Gly 1165 3504 tge cag atg gte age aac etc gee gaa at atce ccc gge acg gca gge Cys Gin Met Val Set Asn Leu Ala Glu Ile ePro Gly Thr Ala Gly 1170 1175 1180 tgg cog aag ttc aag Cgc aac ctg agc gaa cag tte gaa gcg cgc ctg Trp Pro Lys Phe Lys Arg Asn Leu Sex Glu Gin Phe Glu Ala Arg LeU 3552 3600 WO 01/85772 PCTfGBOI/02003 1185 1190 1195 1200 age atg gtt cac gtc ccc aaa tea gee toe ctg att ctg sac gaa atg Met Val His Val Pro Lys 3cr Ala 3cr Leu Ile LeU Asn Giu Met 1205 1210 1215 3648 esa ggc tee age ctg Gin Sly 8cr Set Lcu 1220 gac tte geg ctt cac Asp Phe Ala LeU His 1235 cccgtc gte gte agc Pro V/al 1/al 1/al 8cr 1225 gge gge sac att tct Sly Sly Ass Ie 3cr 1240 cac ggc gas gge cgc gc His Sly Gin Sly Arg Ala 1230 gee gat ttg ggc att geg Ala Asp Leu Sly Ile Ala 1245 3696 3744 ttg caa tat gtg gac LeU Gin Tyr V/al Asp 1250 gga esa sac Gly Gin Ass 1255 caa att ac eaa ace Gin Ile Thr Gin Thr 1260 tac cg tc Tyr Pro LeU 3792 3840 sac ccc aac ggc Ass Pro Ass Sly 1265 tog ceg can 5cr Pro Sin 1270 ggc ate gcc gge Sly Ile Ala Sly 1275 gtg ace sac V/al Thr Ann gee gac Ala Asp ggC egc gtt ace ate atg atg CCg Sl1y A-rg V/al Thr Ile Met Met pro 1285 cat cet ga His Pro Sin 1290 cgt gta tac ege gcc Arg Vasi Tyr Arg Ala 1295 gas ttg tee ggc tgg Glu Len 5cr Sly Trp 1310 3888 3936 ges eaa stg age tgg aaa A-la GIn Met 5cr Tzp Lys 1300 cg gsa gac tgg acg Pro Gin ASP Trp Thr 1305 tao ege etc tto gee gge ges egt asa gee ttg ggc Tyr Arg Len Phe Ala Sly Ala Arg Lys Ala Len Sly 1315 1320 <210> 38 <211> 1324 <212> PRT <213> Neisseria meningitidis 3972 <400> 38 Val 3cr Arg Ile Met Ser V/al 1/al 1 5 Leu 5cr Asp Phe A-rg V/al Gin Lys Len Pro Leu Arg Sly Val Thr Ala Len LeU Sin Lys Ala Ala Ala Len Sly Len Pro Gin V/al Lys LeU Set Set Sin Phe Trp Tyr Phc V/al (fly WO 01/85772 Ser Glu Lys Ala Leu Asp Ala 55 Leu Ala Ala Gln Ser Val Glu 70 His Leu Phe Leu Val Thr Pro Ser Lys Ala Thr Asn Ile Ala 100 Arg lie Glu Arg Gly Met Ala I 115 1 Glu Gln Gin Gln Gln Trp Ala A 130 135 Ser Val Leu Pro Asp Phe Gln T 145 150 Glu Ser Glu Thr Phe Ser Thr V; 165 Ala Leu Val Lys Ala Asn Thr G1 180 Glu Ile Asp Tyr Leu Val Glu Asj 195 20 Ser Asp Val Glu Leu Met Met Phe 210 215 Arg His Lys Ile Phe Asn Ala Asp 225 230 Pro Lys Set Leu Phe Gly Met Ile 245 Glu Gly Thr Val Val Ala Tyr Lys 260 Ala Lys Val Glu Arg Phe Tyr Pro 275 280 Arg Phe His Glu Glu Asp Thr His 78 PCT/GB01/020 0 3 Ala Thr Val Glu Lys Leu Gln Ala Leu Gln Thr Pro Lys Ala Arg Glu Gly Leu 75 Arg Leu Gly Thr Ile Ser Pro Trp Ala 90 ;lu Asn Cys Gly Leu Ala Gly Ile Glu 105 110 ral Trp Leu Glu Gly Ala Leu Thr Asp .20 125 la Leu Leu His Asp Arg Met Thr Glu 140 hr Ala Ser Lys Leu Phe His His Leu 155 160 l1 Asp Val Leu Gly Gly Gly Lys Glu 170 175 u Met Gly Leu Ala Leu Ser Ala Asp 185 190 n Tyr Gln Ala Leu Gin Arg Asn Pro 0 205 Ala Giln Ala Asn Ser Glu His Cys 220 Phe Ile Leu Asn Gly GIu Lys Gin 235 240 Arg Asp Thr His Asn Ala His Pro 250 255 Asp Asn Ser Set Val ile Glu Gly 265 270 Asn Ala Ala Glu Asn Gin Gly Tyr 285 Ile Ile Met Lys Val Glu Thr His WI Asn 305 Gly Lys Leu I Ser p 3 Asn A 385 Glu G Ile Al Glu Ii Leu Il Asn Asp 465 Ile Glu Asp Lys Ser Asn Phe Lys 530 Gin Ile O 01/85772 290 PCT/GB01/020 0 3 295 His Pro Thr Ala Ile 310 Gly Glu Ile Arg Asp 325 Ala Gly Leu Thr Gly 340 ys Gin Pro Trp Glu 355 ro Leu Asp Ile Met 70 sn Glu Phe Gly Arg P 390 lu Lys Phe Asp Gly G 405 .a Gly Gly Leu Gly S 420 e Pro Glu Gly Ala Le 435 e Gly Leu Gly Gly Gi 45 Ala Set Leu Asp Ph 470 Arg Arg Ala Gln Gil 485 Asn Pro Ile Ile Set 500 Ala Phe Pro Glu Leu 515 Leu Arg Glu Val Pro 535 Trp Cys Ash Glu Ser I 3 E U Ala Pro Phe Ala Gly Ala Ala Thr Gly 315 Glu Gly Ala Thr Gly Lys Gly Ser Arg 330 335 Phe Thr Val Ser Asn Leu Asn Ile Pro 345 350 Gln Asp Tyr Gly Lys Pro Glu His Ile 360 365 Ile Glu Gly Pro Ile Gly Gly Ala Ala 375 380 ro Asn Leu Leu Gly Tyr Phe Arg Thr P 395 4 in Val Arg Gly Tyr His Lys Pro Ile M 410 415 er Ile Gln Ala Gin Gln Thr His Lys A 425 430 u Leu Ile Gin Leu Gly Gly Pro Gly Me 440 445 y Ala Ala Ser Ser Met Asp Thr Gly Th 5 460 e Asn Set Val Gln Arg Gly Asn Pro G 475 481 a Val Ile Asp Arg Cys Trp Gln Leu C3l 490 495 Ile His Asp Val Gly Ala Gly Gly Leu 505 510 Val Asn Asp Ala Gly Arg Gly Ala Val 520 525 Leu Glu Glu His Gly Leu An Pro Leu 540 Gln Glu Arg Tyr Val Leu Ser Ile Leu 79 Ala 320 Pro Gly Set Phe 'he 00 .et sp et r u U 2; WO 01/85772 545 Glu Lys Asp Leu Asp 565 Pro Phe Ala Val Val 580 Arg Asp Asp Leu Phe 595 Leu Leu Gly Lys Pro 610 Pro Set Lys Lys Pro 625 Ala Tyr Arg Val Leu 645 lie Thr Ile Gly Asp A 660 Met Val Gly Lys Tyr G 675 Met Gly Phe Asn Thr T 690 Pro Thr Val Ala Leu Ph 705 71 Gly Glu Ala Ile Thr As 725 Asn Ile Lys Leu Set Al 740 Glu Asp Glu Lys Leu Ty 755 Gln Ala Leu Asp Leu Sex 770 Lys Thr Val Trp Gin Asp 785 790 Le Se Le e e S790r Leu Set Leu Ile Ile Ser 550 555 Ile Phe Arg Ser Ile Cys 570 Gly Thr Ala Thr Asp Asp 585 Ser Asn Asn Pro Val Asp 600 Pro Lys Thr Thr Arg Thr 615 Phe His Ala Gly Asp Ile Z 530 635 Arg Leu Pro Ala Val Ala A 650 rg ser Val Gly Gly Met T 665 In Thr Pro Val Ala Asp C' 680 yr Arg Gly Glu Ala Met Se 695 70 'e Asp Ala Pro Ala Ser Gl .0 715 n Ile Ala Ala Ala Asn Il 730 a Asn Trp Met Ala Ala Cyi 745 r Arg Thr Val Glu Ala Val 760 Ile Pro Val Gly Lys Asp 775 780 Gly Glu Glu Lys Lys Set 795 Ala Phe Ala Pro Val Gin PCT/GB01/02003 560 Glu Arg Glu Arg Cys 575 Gly His Leu Lys Val 590 Leu Pro Leu Asn Val 605 Asp Lys Thr Val Ala 520 tsp Ile Thr Glu Ala 640 la Lys Asn Phe Leu 655 hr His Arg Asp Gin 670 ys Ala Val Thr Met 685 r Met Gly Glu Lys '0 y Arg Met Cys Val 720 e Gly Asp Ile Gly 735 s Gly Asn Glu Gly 750 Set Lys Ala Cys 765 Ser Leu Ser Met Val Val Set Pro 800 Asp Val Arg Lys WO 01/85772 PCT/GB01/020 0 3 1UV 815 Thr Val Thr Pro Glu Leu Lys Assn Val Glu Asp .Ser Val Leu Leu Phe 820 825 830 Val Asp Leu Gly Phe Gly Lys Ala Arg Met Sly Gly Set Ala Phe ly 835 840 845 Gln Val Tyr Asn Asn Met Ser Gly Asp Ala Pro Asp Leu Asp Asp Thr 850 855 860 Set AZg Leu Lys Ala Phe Tyr Asn Val Ile Gln Gln Leu Val Ala Glu 865 870 875 880 Asp Lys Leu Leu Ala Tyr His Asp Arg Set Asp Gly Gly Leu Phe Ala 885 890 895 Val Leu Val Glu Met Ala Phe Ala Gly Arg Cys Gly Leu Asp Ile Asp 900 905 910 Leu Asn Leu Leu Leu Ala Gln Thr Phe Ile Thr Ash His Thr Ala Leu 915 920 925 Set Gln Set Leu Arg Thr Glu Glu Val Lys Ala Leu Ala Glu Trp Gin 930 935 940 Glu Thr Ile Ala Arg Thr Leu Phe Asn Glu Glu Leu Gly Ala Val Ile 945 950 955 960 Gln Val Arg Lys Gin Asp Val Ala Ap Ile Ile ASs Leu Phe Tyr Gn 965 970 975 Gin Gin Leu His His Asn Val Phe Glu Ile Gly Thr Leu Thr Asp Glu 980 985 990 Asn Thr Leu Ile lie Arg Asp Gly Gln Thr His Leu Ile Ser Asp Asn 995 1000 1005 Leu Ile Lys Leu Gin Gin Thr Trp Gln Glu Thr Set His Gin Ile Gln 1010 1015 1020 Arg Leu Arg Asp Ash Pro Ala Cys Ala Asp Set Glu Phe Ala Leu Ile 1030 1035 1040 Gly Asp Asn Gly Arg Ser Ala Leu Phe Ala Asn Leu Lys Phe Asp Val 1045 1050 1055 Asn Glu Asp Ile Ala Ala Pro Phe Ile Asn Ser Gly Ala Lys Pro Lys 81 WO 01/85772 PCT/GB01/02003 1060 1065 1070 Ile Ala Ile Leu Arg Glu GIn Gly Val Asn Gly Gin Ile Glu Met Ala 1075 1080 1085 Ala Ala Phe Thr Arg Ala Gly Phe Asp Ala Tyr Asp Val His Met Ser 1090 1095 1100 Asp Leu Met Ala Gly Arg Val His Leu Ala Asp Phe Lys Met Leu Ala 105 1110 1115 1120 Ala Cys Gly Gly Phe Ser Tyr Gly Asp Val Leu Gly Ala Gly Lys Gly 112 1130 1135 Trp Ala Lys Ser Ile Leu Phe His Pro Ala Leu Arg Asp Gln Phe Ala 1140 1145 1150 Ala Phe Phe Thr Asp Pro Asn Thr Leu Thr Leu Gly Val Cys Asn Gly 1155 1160 1165 Cys Gin Met Val Ser Asn Leu Ala Giu Ile Ile Pro Gly Thr Ala Gly 1170 1175 1180 Trp Pro Lys Phe Lys Arg Asn Leu Se Glu Gln Phe Glu Ala Arg Leu 185 1190 1195 1200 Ser Met Val His Val Pro Lys Ser Ala ser Leu Ile Leu Asn Glu Met 1205 1210 1215 Gin Gly Ser Set Leu Pro Val Val Val Set His Gly Glu Gly Arg Ala 1220 1225 1230 Asp Phe Ala Leu His Gly Gly Asn Ile Ser Ala Asp Leu Gly Ile Ala 1235 1240 1245 Leu Gln Tyr Val Asp Gly Gin Asn Gin Ile Thr Gln Thr Tyr Pro Leu 1250 1255 1260 Asn Pro Asn Gly Ser Pro Gln Gly Ile Ala Gly Val Thr Asn Ala Asp 265 1270 1275 1280 Gly Arg Val Thr Ile Met Met Pro His Pro Glu Arg Val Tyr Arg Ala 1285 1290 1295 Ala Gln Met Set Trp Lys Pro Glu Asp Trp Thr Glu Leu Ser Gly Trp 1300 1305 1310 Tyr Arg Leu Phe Ala Gly Ala Arg Lys Ala Leu Gly 82 WO 01/85772 PCT/G13ol/02003 1315 1320 <210' 39 <211> 471 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (471) <400> 39 atg tee cgc aat ace gas gag etg caa geaetgcttggtst 4 Me 5 r Ar nn Ti in Gn5e G ly Ile 8r Leu Leu ly Ann 1 510 tag Aaa acc ega tat cog acc ggc tac gcg ect gaa att etc gaa gea 96 Gin Lys Tin Arg Tyr Pro Thr Sly Tyr Ala Pro Glu Ile Len Gin Ala 25 tto gao sac ass cat ccc gac sac gao tat ttc gte aaa ttc gtc tge 144 Phe ASP Ann Lys His Pro Asp Asn Asp Tyr Phe Val Lys Phe Val Cys 40 oca gag tte ace age etc tgo ccc atg ace ggg eag ccc gsc tte gee 192 Pro Sin Phe Thr 5cr Leu Cys Pro Met Thr Sly-Gin Pro Asp-Phe Ala 55 ace ate tao ate ege tao att ceg cac ate aaa atg gtg gaa ago ass 240 Ti Ile Tyr Ile Arg Tyr Ile Pro His Ile Lys Met Val Gin 8cr Lys 70 75 tee etg aa ete tao etc ttc age ttc ego sac cac gge gat ttt eat 288 Len Lys Len Tyr Len Phe Set PMe Arg Ann His Sly Asp Phe His 90 gsa gao tge gte sac ate ate atg ass gac etc att gee otg atg gat 336 Giu Asp Cys Val An Ile Ile Met Lys Asp Len Ile Ala Len Met Asp 100 105 110 cg ass tac ate gas gts. tte ggc gag tte sea cog ego ggo ggc ate 384 Pro Lys Tyr Ie Gin Val Phe Gly Gin Phe Thr Pro Arg Siy Sly Ile 115 120 125 gee stt cat cot tte gee sat tae gge as gon gge ace gag ttt gas 432 Ala Ile His Pro Phe Ala Asn Tyr Sly Lys Ala Gly Thr Gin Phe Gin 823 WO 01/85772 130 135 140 gca ttg gcg cgt aaa cgc ctg ttt gag cac gac gca caa Ala Leu Ala Arg Lys Arg Leu Phe Glu His Asp Ala Gln 145 150 155 <210> <211> 157 <212> PRT <213> Neisseria meningitidis <400> Met Ser Arg Asn Thr Glu Glu Leu Gln Gly Ile Ser LeU 1 5 10 Gln Lys Thr Arg Tyr Pro Thr Gly Tyr Ala Pro Glu le I 25 Phe Asp Asn Lys His Pro Asp Asn Asp Tyr Phe Val Lys P 40 Pro Glu Phe Thr Ser Leu Cys Pro Met Thr Gly Gln Pro A 55 Thr Ile Tyr Ile Arg Tyr Ile Pro His Ile Lys Met Val G 70 75 Set Leu Lys Leu Tyr Leu Phe Ser Phe Arg Asa His Gly As 90 Glu Asp Cys Val Asn Ile Ile Met Lys Asp Leu Ile Ala Le 100 105 11 Pro Lys Tyr Ile Glu Val Phe Gly Glu Phe Thr Pro Arg Gl 115 120 125 Ala Ile His Pro Phe Ala Asn Tyr Gly Lys Ala Gly Thr Gl 130 135 140 Ala Leu Ala Arg Lys Arg Leu Phe Glu His Asp Ala Gln 145 150 155 PCT/GB01/02003 Leu Gly Asn Leu Glu Ala 'he Val Cys sp Phe Ala lu Set Lys p Phe His u Met Asp 0 y Gly Ile 4 Phe Glu <210> 41 <211> 504 <212> DNA WO 01/85772 <213> Neisseria menxingitit 5 s <220> <221> CDS <222> (504) <400> 41 fltg ccc cta cta gao agt ttc aaa gta gat cac ac cgt atg cat gco 48 Met Pro Leu Leu Asp 5cr Phe Lys Val ASP His Thr Arg Met His Ala 1 5 10 1 Ccc goc gta cgo gtg gcg asa aac atg aac acg ccc aaa ggc gac acc 96 Pro Ala Val Arg Val Mla Lys Thr Met Thr Thr Pro Lys Gly Asp flat 25 att ace gtg tta gac ctg ago ttt tgc gtt ccc aat aaa gaa ata ctg 144 Ile Thr Val. Phe Asp Leu Arg Pbs Cys Val. Pro Asn Lys Glu Ile Leu 40 cot gaa aaa ggc ata cac acg ctg gag cat ttg ttc gca ggt ttt atg 192 Pro Giu Lys Gly Ile His Thr LeU Giu His Leu Phe Ala Gly Pbs Met 55 ago gac cac ttg aac ggc Rac ggc gtg gaa ata ata gac att tca cog 240 Arg Asp His Leu Asn Gly Asn Gly Val. Glu Ile Ile Asp Ile 5cr Pro 70 75 so atg ggc tgc cgc acc ggt ttt tat atg ago ctt ate ggc acg cat tcc 288 Met Gly Cys Arg Tin Gly Phe Tyr Met Ser Leu Ile Gly Thr Pro Set 90 gaa cag cag gtc gec gat gcg tgg ctg gct tog atg cag gat gtt ttg 336 Gln Gin Gin Val Ala Asp Ala Trp Leu Ala Ser Met Gin Asp Val Leu 100 105 110 aat gto aaa gac aaa aga aaa atc ccc gag ttg sac gaa tac caa tga .384 Asn Val Lys Asp Gin 5cr Lys Ile Pro Gin Len Asn Gin Tyr Gin Cys 115 120 125 ggc act tat caa atg cac tog ata goc gsa gag cag osa ate gag caa 432 Gly Thr Tyr Gin Met His 5cr Len Mla Gin Ala Gin Gin Ile Ala Gin 130 135 140 aaa gtg ttg gag ogo aaa gtg gog gtg aaa aaa aat gaa gag ctg acg 480 Asn Val. Leu Ala Arg Lys Val Ala Val Asn Lys Asn Gin Gin Leu Thr 145 ISO 155 160 ctg gat gaa ggg ctg otg aac gac 504 WO 01/85772 PCT/GB01/02003 Lou Asp Glu Gly Leu Leu Asn Ala 165 <210> 42 <211> 168 <212> PRT <213> Neisseria meningitidis <400> 42 Met Pro Leu Leu Asp Ser Phe Lys Val Asp His Thr Arg Met His Ala 10 Pro Ala Val Arg Val Ala Lys Thr Met Thr Thr Pro Lys Gly Asp Thr Ile Thr Val Phe Asp Leu Arg Phe Cys Val Pro Asn Lys Glu Ile Leu 40 Pro Glu Lys Gly Ile His Thr Leu Glu His Leu Phe Ala Gly Phe Met 55 Arg Asp His Leu Asn Gly Asn Gly Val Glu Ile Ile Asp .Ile Ser Pro 75 Met Gly Cys Arg Thr Gly Phe Tyr Met Ser Leu Ile Gly Thr Pro Ser 90 Glu Gln Gln Val Ala Asp Ala Trp Leu Ala Set Met Gln Asp Val Leu 100 105 110 Ann Val Lys Asp Gln Set Lys Ile Pro Glu Leu Asn Glu Tyr Glin Cys 115 120 125 Gly Thr Tyr Gin Met His Ser Leu Ala Glu Ala Gin Gin Ile Ala Gln 135 140 Asn Val Leu Ala Arg Lys Val Ala Val ASn Lys ASn Glu Glu Leu Thr 145 150 155 160 Leu Asp Glu Gly Leu Leu Asn Ala 165 <210> 43 <211> 813 <212> DNA WO 01/85772 P <213> Neisseria meningitidis <220> <221> cDs <222> <400> 43 atg act atg cay gac cta ccc Oga tao gac gtt ttt ggo aaa ccc gta Met Thr met His Ala Leu Pro Arg Tyr A1a Val Phe Sly Asn Pro Val 10 goc cac ago aaa tag cog osa att cat aaa caa ttt gac ott cag gaa Ala His 5cr Lys Ser Pro Gin lie His Gin Gin Ph. Ala Leu Gin Giu 25 ggC gtt gac att gaa tac gaa cga att tgc gca gao atc gga ggt tta Sly Val Asp Ile GlU Tyr 5 u Arg Ile Cys Mla Asp lie Sly Sly Pie 40 gcg cag gag gtt tog aca ttt ttt gaa aca gga ggt tgc ggg gca any Ala Gin Ala Val 5cr Tir Phe Pie Clu Tir Sly Sly Cys Sly Ala Asn 55 gtt aca gta cog tta aag cag gaa gag ttt cat atg gag gao gag cat Val Tic Val Pro Pie Ly's Sin SWu Ala Pie His Leu Ala Asp Ciii His 6570 75 tat gaa ago gca ttg gat gao g gag gtc aao avg otg att ttt ctg Ser SWu Ang Ala Leu Ala Ala Sly Ala Val Asn Tin Leu Ile Phe Leu 90 aaa aav gga aaa vtg cga ggc gao ant aco gao ggt ato ggt ttg gca Lys Asn Sly Lys Leu Arg Sly Asp Asn Thr Asp Sly Ile Sly Leu Ala 100 105 110 aaa gac ato.ag cag gta aaa aao att goc ata gaa gga nan nyc ato Asn Asp Ile Thr Gin Val Ly's Ann Ile Ala Ile Ghu Sly Lys Tin Ile 115 120 125 ttg att ttg ggc gag gga ggc gag gtg ago gga gtg att ct gtt ttg Lou Leu Leu Sly Ala Sly Sly Ala Val Ang Sly Val Ile Pro Val Leu 130 135 140 aaa gaa caC ogt at gao vgt ata gta att goc aac ogt aca ago gao 4 Lys Glu His Arg Pro Ala Arg Ile Val Ile Ala Asn Arg Thr Arg Ala 145 150 155 160 an gao gag gaa ttg gag cag ott tto gga att gaa goo gto cog atg 87 r/GBOI,02003 288 336 384 432 WO 01/85772 PCT/GB01/02003 Lys Ala Glu Glu Leu Ala Gin Leu Phe Gly Ile Glu Ala Val Pro Met 165 170 175 gcg gat gtg aac gga ggt ttt gat atc ate atc aac ggc acg tcg ggc 576 Ala Asp Val Asn Gly Gly Phe Asp Ile Ile Ile Asn Gly Thr Ser Gly 180 185 190 ggt cta aac ggt cag att ccc gat att ccg ccc gat att ttt caa aac 624 Gly Leu Asn Gly Gin Ile Pro Asp Ile Pro Pro Asp Ile Phe Gin Asn 195 200 205 tgc gcg ctt gcc tac gat atg gtg tac ggc tgc gag gca aaa ccg ttt 672 Cys Ala Leu Ala Tyr Asp Met Val Tyr Gly Cys Ala Ala Lys Pro Phe 210 215 220 tta gat ttt gca aga caa tcg ggt gag aaa a a gcc gac gga ctg 720 Leu Asp Phe Ala Arg Gin Ser Gly Ala Lys Lys Thr Ala Asp Gly Leu 225 230 235 240 ggt atg cta gte ggt caa gcg gcg gat tcc tac gcc ctc tgg cgc gga 768 Gly Met Leu Val Gly Gin Ala Ala Ala Ser Tyr Ala Leu Trp Arg Gly 245 250 255 ttt acg ccc gat ate cgc ccc gtt ate gaa tac atg aaa gcc cta 813 Phe Thr Pro Asp Ile Arg Pro Val Ile Glu Tyr Met Lys Ala Leu 260 265 270 <210> 44 <211> 271 <212> PRT <213> Neisseria <400> 44 Met Thr Met His 1 Ala His Ser Lys Gly val Asp Ile Ala Gin Ala Val Val Thr Val Pro meningitidis Ala Leu Pro 5 Ser Pro Gin I Glu Tyr Glu A Ser Thr Phe P1 55 Phe Lys Gin GI 70 8 \rg Tyr le His 25 .rg Ile 40 he Glu lu Ala 8 Ala 10 Gin Cys Thr Phe Val Gin Ala Gly His 75 Phe Gly Asn Pro Val Phe Ala Leu Gin Glu Asp Ile Gly Gly Phe Gly Cys Gly Ala Asn Leu Ala Asp Glu His WO 01/85772 Ser Glu Arg Ala Leu Ala Ala Lys Asn Gly Lys Leu Arg Gly 100 Asn Asp Ile Thr Gln Val Lys 115 Leu Leu Leu Gly Ala Gly Gly 130 135 Lys Glu His Azg Pro Ala Arg 145 150 Lys Ala Glu Glu Leu Ala Gln I 165 Ala Asp Val Asn Gly Gly Phe A 180 Gly Leu Asn Gly Gln Ile Pro A 195 2 Cys Ala Leu Ala Tyr Asp Met V; 210 215 Leu Asp Phe Ala Arg Gin Ser GI 225 230 Gly Met Leu Val Gly Gln Ala Al 245 Phe Thr Pro Asp Ile Arg Pro Va 260 Gly Ala Asp Asn 105 Asn Ile 120 Ala Val Ile Val Leu Phe 1 Lsp Ile I 185 sp Ile P 00 al Tyr G ly Ala L3 a Ala Se 25 1 Ile G1 265 V T Al Ar Ii 17 le ro ly ya r 0 u al Asn Thr L 90 hr Asp Gly I -a Ile Giu G 1: g Gly Val Ii 140 e Ala Ash Ar 155 y Ile Glu Al 0 Ile Asn Gli Pro Asp Ile 205 Cys Ala Ala 220 Lys Thr Ala 235 Tyr Ala Leu Tyr Met Lye PCT/GB01/02003 5eu Ile Phe Leu le Gly Leu Ala 110 ly Lys Thr Ile le Pro Val Leu g Thr Arg Ala 160 a Val Pro Met 175 y Thr Ser Gly 190 Phe Gln Asn I Lys Pro Phe Asp Gly Leu 240 Trp Arg Gly 255 Ala Leu 270 <210> <211> 546 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> WO 01/85772 PCT/GBOI/o200s <400> ttg ott tot cat ttg gat atg aaa tto gte agc gac ett ttg tot gte 48 Lou Lou Ser His Len Asp Met Lys Phe Val Ser Asp Lou Lou 8cr Val 1 5 10 ate ttg ttt ttt got act tat ace gtt ace aaa aat atg att gee gct 96 Ile Lou Phe Phe Ala flit Tyr Thr Val Thr Lys Asri Met Ile Ala Ala 25 aeg geg gtt gee ttg gtt gee ggt gtg gtt eag geg got ttt etg tat 144 Thr Ala Val Ala Lou Val Ala Gly Val Val Gin Ala Ala Phe Lou Tyr 40 tgg aaa tat aaa aag ctg gat aeg atg cag tgg gtc gga. etg gtg otg 192 Trp Lys Tyr Lys Lys Lou Asp Thr Met Gin Trp Vai Gly Lou Val Leu so 55 att gtc gta tte ggc gge gee ace att gtt ttg gge gee age egc ttc 240 le Val Val Phe Gly Gly Ala Thr Ile Val Len Gly Asp Ser Arg Phe 70 75 att atg tgg aag ceg age gtt ttg ttt tgg etg gge gog ctg tte ctg 288 Ile Met 7'rp Lye Pro Bar Val Lou Phe Trp Lou cfly Ala Leu Phe Lou 90 tgg gge age capcetc gee ggt aaa aae gge ttg aag geg agt ate gge 336 Trp Gly Ser His Lou Ala Gly Lys Asn Gly Len Lys Ala 8cr Ile Gly 100 105 110 agg gag att eag ett cog gat gee gta tgg geg aaa ttg aeg tat atg 384 Arg Gi Ile Gin Lou Pro Asp Ala Vai Trp Ala Lys Lou Thr Tyr Met 115 1120 125 tgg gte ggt ttc etg att ttt atg ggt atc gce ae tgg ttt gtg ttt 432 Trp Vai Gly Phe Le Ile Phe Met Giy Ile Al1a lien Trp Pec Vai Phe 130 135 .140 ace egg ttc gag teg eaa tgg gte aac tat aaa atg ttc gge teg act 480 Thr Arg Phe Gin 5cr Gin Trp Val lisn Tyr Lys Met Phe Giy 8cr Thr 145 150 155 160 gem etg atg ctt gtt tte ttt att att eag ggt att tat ctg agt ace 528 Ala Lou Met Lou Val Phe Phe Ile Ile Gin Gly Ile Tyr Lou Ser Thr 165 170 175 tgt ctg ae aeg gag gat 546 Cys Lou Lys Lys Gin Asp 180 WO 01/85772 PCT/GB0110200 3 <210> 46 <211> 182 <212> PRT <213> Neisseria meningitidis <400> 46 Leu Leu Ser His Leu Asp Met Lys 1 5 Ile Leu Phe Phe Ala Thr Tyr Thr Thr Ala Val Ala Leu Val Ala Gly 40 Trp Lys Tyr Lys Lys Leu Asp Thr 55 Ile Val Val Phe Gly Gly Ala Thr 70 Ile Met Trp Lys Pro Ser Val Leu E Trp Gly Ser His Leu Ala Gly Lys A 1.00 1 Arg Glu Ile Gln Leu Pro Asp Ala V 115 120 Trp Val Gly Phe Leu Ile Phe Met Gi 130 135 Thr Arg Phe GIu Ser Gln Trp Val As 145 150 Ala Leu Met Leu Val Phe Phe Ile 11 165 Cys Leu Lys Lys Glu Asp 180 P V V Me Il Ph 05 al ly n n e he V al T 25 al Va it G1 e Va e Trp 90 i Gly Trp Ile Tyr Gln 170 al Ser Asp L 10 hr Lys Asn M4 I Gln Ala A] 4 n Trp Val GI 1 Leu Gly Asj 75 Leu Gly AlI Leu Lys Ala Ala Lys Leu 125 Ala Asn Trp 140 Lys Met Phe 155 Gly Ile Tyr eu Leu Ser Val et Ile Ala Ala .a Phe Leu Tyr y Leu Val Leu p Ser Arg Phe I Leu Phe Leu Sec Ile Gly 110 Thr Tyr Met Phe Val Phe Gly Ser Thr 160 Leu Ser Thr 175 <210> 47 <211> 585 WO 01/85772 PCT/GBOI/02003 <212> DxA <213> Neisserie ineningitidis <220> <221> CD~S <222> (585) <400> 47 ttg eat att aaa ctg aaa aca ttg Leu Asn Ile Lys Leu Lys Thr Leu tte ttg ccc ttc gao ag ctg gca Leu Leu Pro Phe Ale Thr Leu Ale ttg tgc ac Leu Cys Thr agt tgg Ctg Arg Trp Leu eat got ttt goc gcc cag ccc egc gec -An Ala Phe Ala Ala pro pro Bar Asp 25 get acg Cag aet ttt gao cgg gat ate ASP Thr Gin an Phe Asp Arg Asp Ile 40 geg tag ttg gag Ala 2cr Leu Ala gee ae aat tg Giu LYS Asn Met att gag ggo Ie Giu Gly ttt aat gao gga ttt aaa cog tat gog gac aaa gcc ctt Phe Asn Ala Gly Phe Lys Pro Tyr Ala Asp LYS Ala Lei 192 240 gaa etg cog gee gcg aaa &ea get GIU Met Pro Glu Ala Lys Lys Asp Ceg gag gca gee gco ttt Gin Ala Mla Giu Ala PMe cgt tat Cgt Arg Tyr Arg gag eat gtt ttg Giu Aen Val Leu aaa get ttg att acg ccc gee gtg aaa Lys ASP Leu Ile Thr Pro Glh Val. Lys czag gct gtc ago Gin Ala Val Arg 100 eat act tta Asn Thr Leu ttg aeg eat gao agt Leu Lye Asn Ala Arg 105 gag ata ta acg Giu Ile Tyr Thr 336 caa gae gae Gin Giu Gin att gac ggc Ile Asp G2ly atg ett Met Ile 120 gco ttt tao ggt tog cct gta ggt Ala Phe Tyr Gly Ber Pro Val. Gly 125 aga tta ateaeag aee tog etg agt Arg Len Ile Lys Lye Ser Met Bar 140 cag tao gtc gtt Gin Sar Val. Vai 130 goc eaa eat cog Ala Lys Asn Pro 135 gee eta gcg gte tct tgg at yea Gi Ile Ala Val Bar Trp Thr Ala 145 150 ttg toe ggg aaa atc gog one cat Leu Ser Gly Lye Ile Ala Gin Hiis 155 160 WO 01/85772 PCT/GBOI/02003 cat ctg ccc gag ttt acg gaa gag ttg cgg cgc atc atc tgc ggo ggt 528 His Leu Pro Gin Phe Thr Giu Glu Lea Arg Arg lie Ile Cys Gly Gly 165 170 175 aaa aat ccc gat gcg ggc tgt aaa caa gcc gga cag gtt ggg aaa agg *576 Lys Asn Pro Asp Ala Gly Cys Lys Gin Ala Guy Gin Val Guy Lys Arg 180 185 190 cat cap aaa 5.85 His Gin Lye 195 <210> 48 <211> 195 <212> PRT <213> Neisser <400> 48 Len Asn lie L 1 Leu Cys Thr A Arg Trp Leu As Ile Glu Guy Ph Ala Giu Met Pr, Arg Tyr Arg Git Gin Ala Val Axq 100 Gin Gin Gin le 115 Gin 5cr Val Val 130 Glu Ile Ala Val 145 ia meningitidis ys Len Lys Thr Len 5 3n Ala Phe Ala Ala ?0 ;p Thr Gin Asn Phe 40 e Asn Ala Gly Phe 55 o Glu Ala Lye Lys I 70 I Asn Val Len Lys A r Asn Thr Leu Leu L 1 Asp Gly Met Ile A 120 Ala Lys Asn Pro Ar 135 Ser Trp Thr Ala Le 150 93 Li Pa As Ly ksI ys 05 aa uu eU L :0 p Az s Pr Let 90 Asn Phe Len Ser eu Pro Phe Ala Thr 10 :o Ser Asp Ala Ser g Asp Ile Glu Lye o Tyr Ala ASp Lys n Ala Ala Giu Ala 75 Ile Thr Pro Glu I *Ala Arg Giu Ile T 110 Tyr Giy Ser Pro V 125 Ile Lys Lys Ser M 140 Gly Lye Ile Ala GI 155 Leu Ala Len Ala Asn Met Ala Leu Phe Asn ral Lye 'yr Thr al Gly et Ser n His 160 WO 01185772 PCTIGBol/02003 His Leu Pro Glu Phe Thr Glu Glu Leu Arg Ar ie Ile Cys Sly Sly 165170 175 Lys Asn Pro Asp Ala Sly Cys Lys Sin Ala Sly Gin Val Sly Lys Arg 180 185 190 His Gin Lys 195 <210> 49 <211> 462 <212> DNA <213> Neisseria neningititis <220> <221> cms <222> (462) <400> 49 ttg ctt tgc cg gaa aaa atg tcg ggg atg gcg gga cag tat ceg tac 48 Leu Leu Cys Pro 5Th Lys Met 5cr Sly Met Ala Sly Gin Tyr Pro Tyr 10 ggc gtc egg tcg ggt ttg egg agg aac ggc ttg aaa eth tgg gat att 96 Sly Val Arg 5cr Sly Leu Arg Arg Asn Sly Leu Lys Leu Trp Asp Ile 25 eat ttt aga atg ace cgt ttt atc gtc gee aga tgc ggt tta ttg ttt 144 Hlis Phe Arg Met Thr Arg PMe Ie Val Ala Arg Cys Sly Leu Leu Phe 40 goa ace ctt aaa gga aaa ace atg aag aaa atg ttc gtg ctg ttc tgt 192 Ala Thr Leu Lys Sly Lys Thr Met Lys Lys Met Phe Val Leu Phe Cys 55 atg et9 tte tee tge gee tte tee ett geg geg gta aac ate aat geg 240 Met Leu Phe 5cr Cys Mla Phe Ser Leu Ala Ala Val Asn Ie Asn Ala 70 75 get teg cag cag gag ctg gag geg etg ecg ggc ata gge Oct geg aag 288 Ala 5cr Gin Gin Gin Len Gin Ala Len Pro Sly Ile Sly Pro Ala Lys 90 geg sag gee att gcg gaa tac egt geg eas aac gyt geg ttc aag tct 336 Ala Lys Ala Ie Ala Gin Tyr Arg Ala Gin Asn Sly Ala Phe Lys 94 WO 01/85772 PCT/GB01/02003 100 gta gac gat ttg acc Val Asp Asp Leu Thr 115 aag ctg aag gat cag Lys Leu Lys Asp Gin 130 gct aaa cca gtg ctg Ala Lys Pro Val Leu 145 <210> <211> 154 <212> PRT <213> Neisseria meni gta aag Val Lys 120 tct gtc Ser Val 135 gcg gat Ala Asp 105 110 ggc ate ggc cct gcg gtg ctg gcg Gly Ile Gly Pro Ala Val Leu Ala 125 ggt gcg ccc gca cca aaa ggc cca Gly Ala Pro Ala Pro Lys Gly Pro 140 aaa aaa Lys Lys -ngitidis <400> Leu Leu Cys 1 Gly Val Arg His Phe Arg Ala Thr Leu Met Leu Phe Ala Ser Gin Ala Lys Ala Val Asp Asp 115 Lys Leu Lys 130 Ser Gly Arg Asn Ile Val Met Lys Ser Leu Ala Leu Arg Ala 105 Lys Gly 120 Pro Tyr Asp Ile Leu Phe Phe Cys Asn Ala Ala Lys Lys Ser Leu Ala Val Gly Ala Pro Ala Pro Lys Gly Pro 140 WO 01/85772 Ala Lys Pro Val Leu Pro Ala Asp Lys Lys 145 150 <210> 51 <211> 969 <212> DNA <213> Neisseria meningitidis <220> PCT/GB01/02003 <221> CDS <222> <400> 51 atg tcc gcc aag 1 Met Ser Ala Lys 1 gcc aaa gtc ctg Ala Lys Val Leu ggc gat gtg tcc Gly Asp Val Ser 3 atc gga cga atc 4 Ile Gly Arg Ile J aac atc get tcg a Asn Ile Ala Ser aacc ga gaa gcc Asn Asp Glu Ala I gtc gcc too tat Val Ala Ser Tyr I 100 ctg cgc gtc gtc 4 Leu Arg Val Val j 115 gaa cat cocc aac ace ctc aaa tco cgt ttc Thr Leu Lys Ser Arg Phe gtg atg ctc gac cgc tat Val Met Leu Asp Arg Tyr 25 gaa gcc ccc gtg cog gtg Glu Ala Pro Val Pro Val ggc gga gcg gca aat gtc Gly Gly Ala Ala Asn Val gta ggg ctg ttg tcg gta Val Gly Leu Leu Ser Val gac gcg ctg atg gtg cag Asp Ala Leu Met Val Gin aaa caa ate gcc acc ace Lys Gln Ile Ala Thr Thr 105 110 cag ctt ate cgc ctt gat Gln Leu Ile Arg Leu Asp 125 gaa caa ate aag cgg aaa WO 01/85772 Sic His Pro Asm Anrg 130 PCTGBOIIO2003 Gic Vat Len Giu Gin Ile Lye Arg Lye Tyr Arg 135 140 ate ttg ccc Ie Leu Pro gge ctg teg Gly Lec 5cr aaa ace, gta Lye Thr Vai ISO gge gca ace Giy Ala Thr 195 gge age tgg Gly Sex Trp 210 ege ege eac Arg Arg His gge atg ace Gly Met Thr gee eaa gaa Ala Gin Sic 260 gga. atg gge Sly met Gly 275 tac ctt gce Tyr Lec Ala 290 geg gtt tgc Ala Val Cys ace atg tae Tyr 150 ate Ile ate Ile att Ile Aen gac Asp 230 ttc Phe tae Tyr ggt Sly act Thr ttt Phe 310 tea gee tac gge aaa 5cr Asp Tyr Gly Lye .160 tgg geg aeaa eec gee Trp Ala Lye His Gin 175 get tee gee ae tee Asp Tyr Gin Lys Tyr 190 gee ttg aee gee gtg Gin Lou Lye Gin Val 205 gaa aea gee eaa eac Gin Lye Ala Gin Aen 220 etg ace ege age gaa Lou Thr Arg Ser Giu 240 ett tee eag ccc ace Ile Tyr Gin Pro Tbr 255 ggc gee ace gtc att Sly Asp Thr Val Ile 270 ace atg ccc gae gee Thr Met Pro Sic Ala 285 gtg geg aae etc ggt Val Ale Lye Lou Sly 300 gee ttg tea ggg cea Ala Lou 8cr Giy Gin 320 969 WO 01/85772 Ser Thr Met <210> 52 <211> 323 <212> PRT <213> Neisseria PCT/GB01/02003 meningitidis <400> 52 Gin Gin Val Gly Ile Ser Gin Arg Gly Gly Asp Ala Met Arg Arg Asn Glu Val 135 Tyr Asp 150 Ile Ser Ile Asp Ile Thr Leu Lys Met Leu Ala Pro Gly Ala -Gly Leu Ala Leu Gin Ile Leu Ile Gin Ile Ile Phe 155 Ile Asp 170 Gly Asp Arg Ala Ser Arg Asp Arg Val Pro Ala Asn Leu Ser Met Val Ala Thr Arg Leu 125 Lys Arg 140 Ser Asp Trp Ala Asp Tyr Glu Leu 205 WO 01/85772 Val Gly Ser 210 Leu Arg Arg 225 Glu Gly Met Arg Ala Gin Ala Gly Met 275 Met Tyr Leu 290 Thr Ala Val 305 Ser Thr Met Asn Glu Asn Asp 215 Asp Leu Thr Ala 230 Phe Ser Glu Gly Tyr Asp Val Ser 265 Gly Leu Ala Ala 280 Thr Ala Ala Gly 295 Phe Ala Glu Leu 310 Thr Leu 235 Pro Ala Cys Val Lys 315 PCT/GB01/02003 Asn Glu 240 Thr Ile Ala Gly Gin 320 <210> 53 <211> 864 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 53 atg aaa gca aaa atc ctg act tec Met Lys Ala Lys Ile Leu Thr Set 1 5 age ctg ttt gcc caa acg ctg gca Ser Leu Phe Ala Gin Thr Leu Ala agt tcc gtc att gat gcg cag gtt Ser Ser Val Ile Asp Ala Gin Val 40 99 gtt gca ctg ctt gcc tgt tec ggc Val Ala Leu Leu Ala Cys Set Gly 10 ace gtc aac ggt cag aaa atc gac Thr Val Asn Gly Gln Lys Ile Asp 25 gcc gca ttc cgt gcg gaa aac age Ala Ala Phe Arg Ala Glu Asn Ser WO 01/95772 ogt goe gaa Arg Ala Gin gtg gto aao Val Val Asn cgg tog go& .Arg Bar Mla aaa aag tog Lys Lye 5cr gag gta aaa Ala Val Lys 115 ana aco caa Lys Thr Gin 130 ato ago ggt Ile 5cr Gly 145 otg ace gao Len Thr Asp gag aaa aaa Ala Lys Lys cgt aoo aaa Arg Thr Lys 195 ttg gaa cag Lou Giu Gin 210 PCT/GBOI/02003 aog cog oaa otg ago aaa tao otg otg gaa aao gaa 192 Thr Pro Gin Len Arg Gin Sc: Lou Len Gin Asn Gin 55 gto gca Val Ala 70 tea eat Lye An gao eag Asp Lys ttg aao Len Asn too gag Ser Gin 135 aaa ggt Lys Gly 150 gaa sat Gin An gat gc Asp Ala ggt gog Gly Ala cg cg Pro Pro 215 oag get Gin Glu gog ott Ala Leu eta aag Lye Pro 105 ggc gag Gly Gin 120 oag gee Gin Gin tog ca Tb: Gin gca aa Ala Lye gto ttg Vai Len 185 cog gto Pro Val 200 ott tat Lou Tyr cog ctg Pro Len aaa ago Lys Arg aaa ttg Lys Lou tto ate Phe Lye tao gog Tyr Ala aaa gao Lys Ale 140 gto cag Val Gin 155 gog gtt Ala Val oat tat Gin Tyr tat gtg Tyr Val got att Ala Ile 220 etc gga Ann Gly 235 240 288 336 384 432 480 528 576 624 672 720 ana ggo gee ttt ag goa tog Lye Gly Gin Phe Thr Ala Thr WO 01/85772 gtt tat tat gtc aac gac age cgc gag gta aaa gtg cct tct ttt Val Tyr Tyr Val Asn Asp Ser Arg Glu Val Lys Val Pro Ser Phe 245 250 255 gaa atg aaa gga cag att gcg ggc aac ctt cag gcg gaa cgg att Glu Met Lys Gly Gin Ile Ala Gly Asn Leu Gin Ala Glu Arg Ile 260 265 270 cgt gcc gtc ggt gca ctg ttg ggc aag gca aac ate aaa cct gca Arg Ala Val Gly Ala Leu Leu Gly Lys Ala Asn Ile Lys Pro Ala 275 280 285 PCT/GB01/02003 gat 768 Asp gac 816 Asp aaa 864 Lys <210> 54 <211> 288 <212> PRT <213> Neisseria meningitidis <400> 54 Val Ala Thr Val Ala Ala Arg Gin Glu Val Leu Ala Pro Ser 105 Glu Ala Glu Val Leu Ala Gly Gin Arg Ala Leu Leu Arg Leu Leu Arg Lys Thr Ala Leu 125 Ala Ala 140 Gin Glu Val Gin Leu Gly Glu Ile WO 01/85772 Leu Thr Asp Ala Lys Lys Arg Thr Lys 195 Leu Glu Gin 210 Lye Gly Glu 225 Val Tyr Tyr Glu Met Lys Arg Ala Val 275 Lys Glu 165 Gly Phe 180 Gin Thr Gly Val Phe Thr Val Asn 245 Gly Gln 260 Gly Ala Glu Asn Asp Ala Gly Ala Pro Pro 215 Ala Thr 230 Asp Ser Ile Ala Leu Leu Ala Lys Val Leu 185 Pro Val 200 Leu Tyr Pro Leu Arg Glu Gly Asn 265 Gly Lys 280 PCT/GB01/02003 Leu Lys 175 Asn Asp Lys Asp Leu Lys Tyr Gly 240 Phe Asp 255 lie Asp Ala Lys <210> <211> 1257 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1257) <400> atg aaa cag acc gtc ctc aaa aat aac ctg caa aac ctg ctt gaa age Met Lys Gin Thr Val Leu Lys Asn Asn Leu Gin Asn Leu Leu Glu Ser 1 5 10 gca gaa aat atc ctg ctg ctt caa ggc cct gtc ggc gat ttt ttt ctg Ala Glu Asn Ile Leu Leu Leu Gin Gly Pro Val Gly Asp Phe Phe Leu 25 cgc ctt gcc gac tgg ctg act gca aac ggc aaa ace gta cat aaa ttc Arg Leu Ala Asp Trp Leu Thr Ala Asn Gly Lys Thr Val His Lys Phe 40 102 WO 01/85772 sac ttt ast gca Asn Phe Asn Mla acc gtt gtt ttt Thr Val. Val Phe gsa tac atc act Giu Tyr Ile Thr aea ego oct tat Thr Arg Pro Tyr 100 gee agt tte tgg Ala 5cr Phe Trp 115 see tts gas ass Thr Leu Giu Lye 130 gce gao ttt ttt Ala Asp Pha Phe 145 aaa gcg eca aeg Lye Ala Pro Thr get ate egt tao Ala Ile Arg Tyr 180 gao tao ate ose Asp Tyr Ile His 195 tgg tcg etc tee Trp 5cr Leu Ser 210 PCT/GBO1/02003 ggc gao gao tat ttt tat cog ccc act cas gog oat 192 Gly Asp Asp Tyr Phe Tyr Pro Pro Thr Gin Ala His 55 cot sag Pro Lys ggt ttt Gly Phe 170 tte egC Phe Arg 185 sat gee Asri Ala ttg sac Leu Asn ggo ass Gly Lye caa Gin gao Asp ea Gin ate Ile egt Arg tat Tyr 160 sac Asn ccc Pro cog Pro ate Ile att Ile 240 055 ate gee as ogt gtg gas Gin Ile Ala Lys Arg Val. Giu 225 230 WO 01/85772 WO 0185772PCT/GBO1/02003 gtt cc ttg eag gta Val Pro Leu Gin Val 245 ttt Ccc &gc gte ega Phe Pro Sex Val Arg 260 ttc sac gac agc Phe Asn Asp Sex css gtc Gin Val 250 cgt ate cat Arg le His tgc gac Cys Asp 255 agc ttc ctg Ser Phe Leu cat gtt ttg agt tca ttt gc His Val Leu Sex Sex Phe Ala 270 gaa cac gcg cct gcc gat cc Giu His Ala Pro Ala Asp Tht ate ate ate Ile Ile Ile sag cat Lys Hi s 285 cat cot stg His Pro Met gac cgc Asp Arg 290 ggc ttt ate gac Sly Phe Ile Asp tgg cgc gac att Trp Arg Asp Ile cgc ttt ate ass Arg Phe Ile Lys cac ccc gas ete His Pro Siu Leu ggc cgt gtg att Sly Arg Val Ile gtc cat gat gtc Val His Asp Val ctg ccc gtt ttc Leu Pro Val Phe cge cac ggt etc ggc atg gtt acc atc aac ago Arg His Sly Leu.Gly Met Val Thr Ile Asn Sex 1008 ace ago ggc ctg tcc gga ctg att cac aat atg cca gtt Thr Ser Sly Leu Ser Sly Leu Ile His Asn Met Pro Val sag gtt ctc Lys Val Leu 350 sat aco ttg Asn Thr Len 1056 ggc cgt gee Sly Arg Ala 355 tat tat gat att Tyr Tyr Asp Ile ggc att act gac Sly Ile Thr Asp 1104 gca gas Ala Sin 370 ttt tgg sat cat Phe Trp Asn His aos cog oct gao Thr Pro Pro Asp gag ctg ttc cat Sin Lieu Phe His 1152 tao cgs atg tao Tyr Arg Met Tyr etc sac gtg acc cas att sac ggc sac ttc Len Asri Val Thr Gin Ile Asn Sly Asn Phe 1200 tao agt cag gtc ttt ttt Tyr 5cr Sin Val Phe Phe cct aao ass aso Pro Asa Lys Asn soa too gac tot Thr Ser Asp 5cr tcc aca Sex Thr 415 1248 ces sca sc Pro Thr Thr 1257 WO 01/85772 <210> 56 <211> 419 <212> PRT <213> Neisseria PCT/GBO1/02003 meningitidis <400> 56 Met 1 Ala Arg Asn Thr Glu Thr Ala Thr Ala 145 Lys Ala Asp Trp Lys Glu Leu Phe Val Tyr Arg Ser Leu 130 Asp Ala lie Tyr Ser 210 Gin Asn Ala Asn Val Ile Pro Phe 115 Gin Phe Pro Arg Ile 195 Leu Thr Ile Asp Ala Phe Thr Tyr 100 Trp Lys Phe Thr Tyr 180 His Ser Len Lys Len Leu Len Thr Asp Asp Asp Asn His His Val Ile Phe Glu Gly Val 135 Glu Gin 150 Val His Ile Glu Arg Ala Leu Lys 215 WO 01/85772 Gin Ile Ala 225 Val Pro Leu Phe Pro Ser Glu His Ala 275 Asp Arg Gly 290 Glu His Pro 305 Leu Pro Val Thr Ser Gly Gly Arg Ala 355 Ala Glu Phe 370 Ala Tyr Arg 385 Tyr Ser Gin Pro Thr Thr Arg Val 245 Arg Ala Ile Leu Leu 325 Ser Tyr Asn Tyr Phe 405 Val Glu 230 Phe Asn Ser Phe Asp Thr Asp Tyr 295 Lys Gly 310 Arg His Gly Leu Asp lie His Pro 375 His Leu 390 Phe Pro Ala Gly Lys Tyr 235 Asp Ser Gin Val 250 Leu Leu His Val 265 Asn Ile Ile Ile 280 Trp Arg Asp Ile Arg Val .le Tyr 315 Gly Leu Gly Met 330 Ile His Asn Met 345 Pro Gly Ile Thr 360 Thr Pro Pro Asp Asn Val Thr Gin 395 An Lys Asn Thr 410 Gly Lys Arg Ile Leu Ser Lys His 285 Lys Arg 300 Val His Val Thr Pro Val Asp Gin 365 Lys Glu 380 Ile Asn Ser Asp PCT/GB01/02003 Ile 240 Asp Ala Met Lys Pro 320 Ser Leu Leu His Phe 400 Thr <210> 57 <211> 1407 <212> DNA <213> Neisseria meningitidis <220> WO 01/85772 <221> CDS <222> (1407) PCT/GBO1/02003 <400> 57 atg acc Met Tbr 1 ogo cgc Arg Arg tog gca Ser Ala ttt gcg Phe Ala gcg tgg1 Ala Trp cgc aaa Arg Lys I gcc tca Mla Set ate ggg Leu Gly ttc ggc Phe Gly)1 130 gca gtg Ala Val 1 145 ttc aaa Phe Lys ccc age Pro Arg aaa ago Lys Set ctg ace Leu Thr aac gao Asn Mla cot ttg Pro Leu ttc ace Phe Thr ccc tao Pro Tyr ctg aag Leu Lys gga cgc Gly Arg 135 tgg ggc Trp Gly 150 gee aac Gilu Asn etc ogo ca Ile Arg Gin ctt ttg gca Leu Leu Ala cat ctt gcc His Leu Mla tao cog tgg Tyr Pro Trp gcg tgg etc Ala Trp Leu cag Ceg gtc Pro Gin Val agg ctg ate Arg Leu Ile 110 ttg ggt atg Leu Gly Met 125 cag gtc ggc Gin Vel Gly cac gge ttg His Gly Leu Gly Mla Mla 175 WO 01/85772 WO 0185772PCT/GBO1/02003 ggt ttg gca gcc geg ttc aac gcg ccg ctg geg ggc gtg Gly Leu Ala Ala Ala Phe Asn Ala Pro Leu Ala Gly Val att ttc gee Ile Phe Ala 190 agg caa att Arg Gin Ile att gag gaa Ile Glu Glu 195 cte ggg cgc ggc .Leu Gly Arg Gly atg ttg cgc tgg Met Leu Arg Trp ctt ttg Leu Leu 210 ggc gtg etc gcc tcc ggt ttc ata cag Sly Val Leu Ala Ser Sly Phe Ile Gin 215 gee att cag ggc Ala Ile Gin Gly aac ceg tat ttt Aan Pro Tyr Phe ggc tte aac ggc Sly Phe Asn Sly gta ttg gaa cat Val Leu Giu His ttt ctg tgg gtc Phe Leu Trp Val ctg tea ggc ctg Leu 5cr Sly Leu tgc gge geg geg Cys Gly Ala Ala gge ggg Sly Sly 255 ctg tte gga Leu Phe Giy aag ata cgc Lys Ile Arg 275 atg ggg ctg Met Gly Leu 290 ttg etc tat ege Leu Leu Tyr Arg ggt geg geg gag ttt gca ccg cge Gly Ala Ala Ala Phe Ala Pro Arg 265 270 gge ttc atc Sly Phe Ile cgc aac cgt ccg ctg ctg etg Arg Asn Arg Pro Leu Leu Leu gcg gca etg Ala Ala Leu etg ctc gcc ctg ctc ggc acg ttc Leu Leu Ala Len Leu Sly Thr Phe 295 eaa ggc aaa acc Gin Gly Lys Thr ggc aec gge tac Gly flit Gly Tyr gaa gee gcc caa Glu Ala Ala Gin ctg cac ggc ate Len His Sly Ile 960 gaa gee eec tta Gin Ala Pro Phe etc gcc gee gcc Len Al L Ala Ala tgg etc gee Trp Leu Ala ace gta tto Thr Val. Phe 335 1008 agc tat tgg gca ggc gtt ceg gga gge att tte act ccc Ser Tyr Trp Ala Gly Val Pro Gly Gly Ile Phe Thr Pro tog ctg Set Len 350 1056 eta ggc gczg Ile Gly Ala 355 gtt ttg gge gag Val Leu Sly Gin ate gao gc Ile Ala Ala ate gao Ile Ala 365 gac ata tog Asp Ile Ser 1104 WO 01/85772 cag ggt gca aac Gin Gly Mla Asn 370 ggc gcg aca caa Gly Ma Thr Gin 385 acg ggc gga caa Thr Gly Gly Gin gcc tcg cap gtt Ala Sex Gin Val 420 tcg gga atg cgc Sex Gly Met Arg 435 acc ggc aat gcg Thr Gly Asn Ala 450 atg ccg tct gaa Met Pro Ser Glu 465 <210> 58 <211> 469 <212> PRT <213> Neisseria <400> 58 Met Thr Ser Thr 1 Arg Arg Leu Sc Ser Ma Leu Val The Ala Leu Glu Ala Trp Val Ala PCT/GBO1/02003 ctg gog 1152 Leu Ala gaa atg 1200 Glu Met 400 att ttc 1248 Ile Phe 415 cac gca 1296 His Ala gcc caa 1344 Ala Gin acg gga 1392 Thr Gly 1407 meningitidii Phe Pro Arg 5 Arg Lys Sex Ala Leu Thr Leu Asn Ala 55 Leu Pro Leu Arg Leu Ala 10 Ile Ala Phe 25 Ala Leu Phe .40 Lys Leu Val Gly Leu Pro 109 Lys Ile Mxg Gin Phe Leu Leu Ala Aa His Leu Ala Gin Tyr Pro Trp Ile Ala Trp Leu WO 01/85772 Arg Lys Phe Ala Ser Leu Leu Gly Gin 115 Phe Gly Ala 130 Ala Val Met 145 Phe Lys Gly Gly Leu Ala Ile Glu Glu 195 Leu Leu Gly 210 Asn Asn Pro 225 Phe Leu Trp Leu Phe Gly Lys Ile Arg 275 Met Gly Leu 290 Tyr Gly Thr 305 Ala Pro Phe Thr Ala Gly Set Gly Ile Pro Gin Val Ser Leu Pro 100 Thr Leu Leu Ser Ile Gly Gly Ala Trp 150 Met Gin Glu 165 Ala Ala Phe 180 Leu Gly Arg Val Leu Ala Tyr Phe Ser 230 Val Ala Leu 245 Arg Leu Leu 260 Gly Phe Ile Leu Leu Ala Gly Tyr His S 310 Tyr Gly Ala 105 Lys Ile Pro 120 Arg Glu Gly 135 Gly Ala Trp Asn Asp Leu Asn Ala Pro 185 Gly Ile Met 200 Ser Gly Phe 215 Gly Phe Asn Ser Gly Leu Tyr Arg Gly 265 Arg Asn Arg 280 Leu Leu Gly 295 Glu Ala Ala Ala Ala Ala 110 Gln Lys Leu Thr Pro Set Cys Lys 155 Met Ala 170 Leu Ala Leu Arg Ile Gin Gly Gly 235 Val Cys 250 Ala Ala Pro Leu Thr Phe Gln Ala 315 Thr Arg Phe Leu 125 Val Gin 140 Lys His Ala Gly Gly Val Trp Glu 205 Val Ala 220 Val Leu Gly Ala Ala Phe Leu Leu 285 Tyr Gin 300 Leu His PCT/GB01/02003 Ile Arg Leu Ala Ala 160 Gly Ala Ile Gly Ile 240 Gly Arg Leu Thr Tyr 320 Glu Ala Pro Phe Gly Leu Lys Trp Leu Ala Thr Val Phe WO 01/85772 PCT/GB01/02003 Ser Ile Gin Gly 385 Thr Ala Ser Thr Met 465 Trp Ala 340 Ala Val 355 Ala Asn Thr Gin Gly Gin Gin Val 420 Met Arg 435 Asn Ala Ser Glu Gly Ile Phe 345 Ile Ala Ala Ile Cys Met Ser Ala Val 395 Trp Met Leu 410 Ser Pro Arg 425 Val Leu Gin Gin Thr Ala Thr Pro Ser 350 Ile Ala Asp 365 Ala Ala Phe 380 Val Val Met Ile Ala Cys Pro Phe Tyr 430 Glu Thr Ala 445 Asn SeC Lys 460 <210> 59 <211> 423 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 59 atg aca caa gaa acc get ttg ggc gca gca ctg aaa tcc gcc gtc caa Met Thr Gln Glu Thr Ala Leu Gly Ala Ala Leu Lys Ser Ala Val Gin 1 5 10 act atg age aaa aag aaa cag aca gaa atg att gcc gac cac ate tac Thr Met Ser Lys Lys Lys Gln Thr Glu Met Ile Ala Asp His Ile Tyr 25 111 WO 01/85772 PCT/GB01/02003 ggc aaa tat gat gta ttc aaa cga ttt aaa ccg ttg gca etc ggt atc Gly Lys Tyr Asp Val Phe Lys Arg Phe Lys Pro Leu Ala Leu Gly lie 144 gat Asp gca Ala ttg Leu gaa Glu cag Gin tot cag gat Gin Asp cgc gtc Arg Val gcg cgc Ala Arg gtt acc Val Thr cag gct Gin Ala 115 gtt gaa tac gat gcc gca Tyr Asp Ala Ala ccg cgc tat ctg Pro Arg Tyr Leu aac aac cgt ttc Asn Asn Arg Phe caa aac cat cct Gin Asn His Pro 110 get gtc gcc gaa Ala Val Ala Glu 125 aca aca gaa Ser Val Glu Ala 130 <210> <211> 141 <212> PRT <213> Neisseria <400> Met Thr Gin Glu 1 Thr Met Ser Lys Gly Lys Tyr Asp Asp Gin Asp Leu Glu Ala Glu Ser Ser Thr Thr Glu 140 meningitidis Thr Ala Leu Gly 5 Lys Lys Gin Thr Val Phe Lys Arg 40 Ile Ala Ala Leu 55 Ala Ala Leu Lys Ser Ala Val Gin 10 Glu Met Ile Ala Asp His Ile Tyr 25 Phe Lys Pro Leu Ala Leu Gly Ile Pro Gin Tyr Asp Ala Ala Leu Ile WO 01/85772 Ala Arg Val Leu Ala Arg Glu Val Thr Gln Gln Ala 115 Ser Val Glu 130 I I f <210> 61 <211> 1377 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1377) <400> 61 atg ttc got ttec aaa tcc tta c Met Phe Ala Phe Lys Ser Leu I 1 gcc gtc gtc gtc got ctg att c Ala Val Val Val Ala Leu Ile ttg gag tgg ctg ccg cat atg t Lou Glu Trp Leu Pro His Met f att ttg tac ggc ttg gcg cgc Ile Leu Tyr Gly Leu Ala Arg C ggc atg ata ggc gcg ttg aat c Gly Met Ile Gly Ala Leu Asn c ttc ttc atc ggg ctg atg gtc z Cys Phe la ser L20 flu cte c Leu j Ala tcc etr ggt Gly :ag a ;1n i agc 113 PCT/GB01/02003 Tyr Leu Lys Ala Arg Phe Lys Gly His Pro Phe Val 110 Ala Glu Thr Pro 125 Glu WO 01/85772 Phe Phe Ile Gly cog acg ctg atg Pro Thr Leu Met 100 tat ttt tcc gcc Tyr Phe Ser Ala 115 agc agc ctg aco 3cr Lou Thr 130 gcg gcg gcg ttt Ala Ala Ala Phe 145 tcg ggc gca ttt Gly Ala Phe ggc att toc gcg Sly Ile Ser Ala 180 aat atg atg tac Asn Met Met Tyr 195 atg Otg tgg ctt Met Lieu Trp Leu 210 gaa tcc ttc cgc Glu 5cr Phe Arg 225 tog ctg att cog Sex Leu Ile Pro aat gcc gtg gtc Asn Ala Val Val 260 acg tat ctg cac tgt Cys 120 act Thr atg Met aaa Lys I ggt Gly ccc Pro 200 gtc Val gaa GJ-u ttg LeuI ttt I Phe gat 114 Ser ccg Pro gtg Val 125 ttt Phe ggo Sly too 3cr gaa GSu agt Ser 205 ttg Leu gtg Val ttg Lieu gcc Ala ggc PCT/GB01102003 Ile ttt 336 Phe ggc 384 Gly atg 432 Met gtt 480 Va1 160 acg 528 Thr aaa 576 Lys ctg 624 Leu gtc 672 Val tat 720 Tyr 240 gto 768 Val gta 816 Val. ttt 864 WO 01/85772 Thr Tyr Leu 275 tac ggc ggo Tyr Gly Gly 290 etc att tot Lou Ile 5cr 305 gtg att ott Val Ile Lou att cot too Ile Pro 3cr ogt goc aca Arg Ala Thr 355 ato ggo gag Ile Gly Glu 370 ot gtt tao Pro Val Tyr 385 ctg gaa gat Leu Glu Asp tgo ggc gtg Cys Gly Val otg cog tat Leu Pro Tyr 435 His Snr Tbr Pro aaa Lys 9gC Sly atg Met 325 ctg Lou ago Snr tat Tyr aag Lys ggg Gly 405 ate Ile ttt Phe otc Lou ggt Gly 310 ago 3cr got Asp gtt Val ttg Lou Cto Leu 390 oog Thr ago 3cr ttc Phe gao Giu 295 ttg Lou ott Lou gc Ala gc Ala agt 5cr 375 ggt Gly gto Vol coo His tgc Cys Asp 260 9gC Sly gaa Glu ggC Sly gto Val atg Met 360 ott Ile otg Lou ato Ile gog tat Tyr 440 Lou Arg gaa gog Giu Ala ogt otg 3cr Met ggg ctg Sly Lou 330 ogo ago Arg 3cr 345 act tog Thr 5cr ttg ttg Lou Lou oat tog His 5cr aac cog Asn Pro *410 otg ggo Lou Sly 425 ttg ogt Lou 5cr Gli Lou Sly Ala 285 ttt aaa gao ott Phe Lys Asp Ile 300 ttt tto oog cog Phe Pho Thr Gin 315 otg ttt goa otg Lou Phe Ala Lou ttt ttg acg aat. Phe Lou Thr Asn 350 gto ggg gtt oat Val Sly Val Asn 365 tog ggt gaa aog 3cr Gly Glu Thr 380 ogo aat otg tog Arg Asn Lou 3cr 395 etc gta cog tgg Lou Vol Pro Trp gtg cog gtt tgg Vol Pro Vol,Trp 430 ttg got ttg aco Leu Ala Lou Thr 445 PCT/GB01102003 Phe aaa 912 Lys oto 960 Ile 320 gog 1008 Ala ggg 1056 Sly ctg 1104 Leu 000 1152 Lye acg 1200 Thr 400 gta 1248 Val tat 1296 Tyr tto 1344 Leu ttc ggt tgg ocg ggg otg act ttg ogc aaa 000 Phe Sly Trp Thr Gly Lou Tb; Lou 5cr Lys Lys 1377 .WO 01/85772 <210> 62. <211> 459 <212> PUT <213> Neisseria PCT/GBO1/02003 meningitidis <400> 62 Met Phe I 1 Ala ValI Leu Glu Ile Leu Gly Met Ph. Ph. Pro Thr I Tyr Phe Ser 1 130 Ala Mla 1: 145 Gly Gly Ile Asn Met Dr I Met Leu I1 5cr Leu Lieu Ile His Met Ala Arg Lieu Asn Met Val Tyr Galy Ala Lieu Cys Ala 135 Ala Asp 150 Gly Asp Ile Val Thr Ile Pro 5cr 215 205 lieu Asn Ser Val Ala Ala Gin Asp 220 WO 01/85772 PCT/GB01/02003 Glu Ser Phe Arg Set Gin Leu Glu Ala Thr Gly Leu Val His Cys Tyr 225 230 235 240 Ser Leu Ile Pro Phe Ala Leu Leu Val Val Leu Ala Leu Met Arg Val 245 250 255 Asn Ala Val Val Ala Met Leu Phe Thr Val Ile Ala Ala Val Ala Val 260 265 270 Thr Tyr Leu His Ser Thr Pro Asp Leu Arg Gin Leu Gly Ala Trp Phe 275 280 285 Tyr Gly Gly Tyr Lys Leu Glu Gly Glu Ala Phe Lys Asp Ile Ala Lys 290 295 300 Leu Ile Ser Arg Gly Gly Leu Glu Ser Met Phe Phe Thr Gin Thr Ile 305 310 315 320 Val Ile Leu Gly Met Ser Leu Gly Gly Leu Leu Phe Ala Leu Gly Ala 325 330 335 Ile Pro Ser Leu Leu Asp Ala Val Arg Ser Phe Leu Thr Asn Ala Gly 340 345 350 Arg Ala Thr Phe Ser Val Ala Met Thr Ser val Gly Val Asn Phe Leu 355 360 365 Ile Gly Glu Gin Tyr Leu Ser Ile Leu Leu Ser-Gly Glu Thr Phe Lys 370 375 380 Pro Val Tyr Asp Lys Leu Gly Leu His Ser Arg Asn Leu Ser Arg Thr 385 390 395 400 Leu Glu Asp Ala Gly Thr Val Ile Asn Pro Leu Val Pro Trp Ser val 405 410 415 Cys Gly Val Phe Ile Ser His Ala Leu Gly Val Pro Val Trp Glu Tyr 420 425 430 Leu Pro Tyr Ala Phe Phe Cys Tyr Leu Ser Leu Ala Leu Thr Leu Leu 435 440 445 Phe Gly Trp Thr Gly Leu Thr Leu Ser Lys Lys 450 455 <210> 63 WO 01/85772 WO 0185772PCT/GBI/02003 <211> 1098 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1098) <400> 63 atg gca ggc aac act ttc gga caa ctc ttc ace gtt acc act ttc ggc Met Ala Sly Ann Tb: Phe Gly Gin Leu Pec Thr Val Thr Thr Pec Sly gas ago cat ggt gog ggt ttg ggc Glu 5cr His Gly Ala Gly Lcu Gly atc atc gac ggc Ile Ile Asp Gly tgc Ccg Ccc Cys Pro Pro ggc ttg ga& Gly Leu Glh tta agc gaa gcg Leu 9cr Giu Ala gat ate tea ttt gac ctc gac cga cgc Asp Ile Gin Pec Asp Lcu Asp Arg Arg 40 gtt aco caa cgc cgc gee gcc gac caa Val Tb: Gin Arg Arg Giu Ala Asp Gin aa&, ccc Lys Pro ggc acc agc cgc Gly Thr 5cr Arg ate ctc tcc ggc Ile Lou 5cr Sly gae Giu gta ttc gaa ggc aaa act ae gt atg Val Pec Giu Giy Lys Thr Tb: Giy Thr etc gcc cto tta atc tgc eat act gad Ile Ala Lcu Leu Ile Arg Asn Tb: Asp aac ae gcc ace agc: ttc cgc ccc ggo Ann Ice Ala Tb: 5cr Phe Arg Pro Sly 100 105 ceg cgc Gin Arg 90 agc aaa get 5cr Lys Asp tat ggc Tyr Giy cac gcc gac tat His Ala Asp Tyr ae tat tgg Tb: Tyr Trp cao aaa tao His Lys Tyr 115 ggc acg cgo gao Giy Th: Arg Asp tao ogg ggc ggc ggc egg agt ttc gco Tyr Arg Giy Sly Sly Arg Se: Sc: Ala 120 125 gcc gcc gge gcc gtt gcc eaa eaa tgg Ala Ala Gly Ala Val Ala Lys Lys Trp cgc gee Arg Slu 130 ace gcc gee cgc Tb: Aia Ala Arg aae gaa eaa ttc ggc acg gae etc acc gcc tac gtt act cee Lye Glu Lys Phe Sly Tb: (flu Ile Tb: Ala Tyr Val Thr Gin WO 01/85772 PCT/GBOI/02003 ggc gaa aaa gaa Gly Giu Lys Glu egg ttt gaa ggc Arg Phe Glu Gly tgc gas Cys Glu 170 eac att tee His Ile Ser oaa aat Gin Asn 175 oct ttt ttt gc gce aae cat age caa Pro Phe Phe Ala Ala Asn His Ser Gin att gcc gag etg gaa aae tat Ile Ala Glu Len Glu Asn Tyr 576 atg gac agc gtg cgo aaa tcc Met Asp 8r Val Arg Lys Set 195 att gaa gcc gec aac gtc ccc Ile Glu Ala Ala Asn Val Pro 210 215 ttg gat Len Asp 200 tee gte gge Ser Val Gly gcg aag etg cat Ala Lys Leu His 205 ccc gta tte gao Pro Val Phe Asp gtc ggc ttg ggo Val Gly Len Gly ctc gat gcc gaa Len Asp Ala Glu gee tao gee atg Ala Tyr Ala Met ggc ate sac gee Gly Ile Awn Ala 720 aaa ggc gtg gaa ate ggc gea ggt ttt Lys Gly Val Glu Ile Gly Ala Gly Phe ago gta acq et Val Thr caa cgo ggc Gln Arg Gly 255 too aac cac Ser Asn His 270 age gaa cac gge gac gas etc ace ceg caa gge ttc ctg Set Glu His Gly Asp Glu Leu Thr Pro Gin Gly Phe Leu tea gge gge Set Gly Gly 275 atc etc gge gge Ile Len Gly Gly age ace gga caa gac ate eac gte 5et Thr Gly Gin Asp Ile His Val 285 sat atc Asn Ile 290 gee atc aaa ccc Ala lie Lys Pro age tee ate gee Ser 5et Ile Ala cog ego ego agt Pro Arg Arg Ser gac ate aae Asp Ile Asn gge aao Gly Asn 310 eec atc gaa etc gee aeg eac gge agg Pro Ile Giu Leu Ala Thr His Gly Arg 315 gac ecc tgc gte Asp Pro Cys Val gge ttg ege gee gcg ccg att gca Pro Ile Ala Gly Len 325 Arg Ala Ala gaa got atg ctg Glu Ala Met leu 335 1008 gca ttg gtt Ala Leu Val ate gac eac gee etg cgc cat ege geg caa aat gcg Ile Asp His Ala Leu Arg His Arg Ala Gin Asn Ala 1056 WO 01/85772 gat gtt cag gtt aat acg ccc gac att ace ctt tea sac aaa Asp Val Gin Val Asn Thr Pro Asp Ile Thr Leu Ser Asn Lys 355 360 365 PCT/GBOI/02003 1098 <210> 64 <211> 366 <212> PRT <213> Neisseria meningitidis <400> 64 Met 1 Glu Gly Lys Val Ile Asn His Arg Leu 145 Gly Pro Sly Asn His Gly Glu Leu Gly Thr Ile Leu Lou Leu Ala Thr 100 Tyr Gly 115 Thr Ala GLU Lys Lys Glu Phe Ala 180 Phe Gly Gly Leu Glu Ala Arg His Gly Val Arg Asm Phe Arg Arg Asp Arg Val 135 Gly Thr 150 Arg Phe Asn His Thr Giy Lou Glu Thr Lys Tyr Arg 125 Ala Val Tie Ile Aa Giu Leu Glu Asn Tyr 190 WO 01/85772 Met Asp Ser 195 Ile Glu Ala 210 Arg Leu Asp 225 Lys Gly Val ser Glu His Ser Gly Gly 275 Asn Ile Ala 290 Ile Asp Ile 305 Asp Pro Cys Ala Leu Val Asp Val Gin 355 Set Leu Asp Ser Val Gly Ala Lys Leu PCT/GB01/02003 His Val Arg Lys Ala Asn Val Ala Glu Ile 230 Glu Ile Gly 245 Gly Asp Glu 260 Ile Leu Gly Ile Lys Pro Asn Gly Asn 310 Val Gly Leu 325 Leu Ile Asp 340 Val Asn Thr r 1 r 200 Pro Val 215 Ala Tyr Ala Gly Leu Thr Gly Ile 280 Thr Set 295 Pro Ile Arg Ala His Ala Pro Asp 360 205 Pro Ile Thr Leu Asp 285 Pro His Glu Ala Asn 365 Phe Asp Ala Val 240 Arg Gly 255 Asn His His Val Arg Ser Arg His 320 Met Leu 335 Asn Ala <210> <211> 461 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> atg caa age ggt ttt aac got att ttc agg aat gac acc atg cag gtt 48 Met Gin Ser Gly Phe Asn Ala Ile Phe Arg Asn Asp Thr Met Gin Val 1 5 10 121 WO 01/85772 aca tea aan Thr 5cr Lye ggg eac ago Gly His 5cr oge ggg 000 Arg Gly Pro tog ago ato 5cr Ile act gao tgc Thr Asp Cys ogo gtg ttt Arg Val Phe ttg aaa gaa Leu Lys Giu 115 tao tgt tog Tyr Cys 5cr 130 tgg Trp gto Val ago 5cr gat Asp cgt Arg ace Thr 100 tog Ser got Ala gga Gly gag Gin gaa Giu 55 atg Met gtt Val cac His gag Gin atg Met 135 atg Met ggg Gly 40 atg Met att le aog Thr ato Ile cgo Arg 120 ttg Lou tgo ttt gto Cys Phe Val tog geg gea 5cr Mla Ala otg ctg ttg Lou Lou LeU goc gaa aan Ala Gin Lye gog aaa egg Ala Lys Arg eat ttc aaa His Phe Lys 105 gee gtt oag Ala Val Gin ggc aaa gog Gly Lys Ala gga aca Gly Thr gaa ggt Gin Gly gge gtg Sly Val cag ogt Gin Arg gog gao Ala Asp gta ttc Val Phe atg tot Met 125 gee eag Mla Lys 140 PCT/GBOI/02003 LggoC 96 iGly :eag 144 kLys tgt 192 Cys agt ttt gaa att gee ggg gee gat aaa ta Phe Gin le 145 <210> 66 <211> 153 <212> PRT <213> Neisseria <400> 66 Met Gin 5cr Giy 1 Thr 5cr Lys Trp, Ala Gly Ala Asp Lys 150 meningitidis Phe Asn Ala Ile Phe Arg Asn Asp Tb: Met Gin Val 5 10 Ile Asp Gly Met Cys Phe Val Gly Thr Thr Gin Gly 122 WO 01/85772 PCT/GB01/02003 Gly Arg Ser Thr Arg Leu Tyr Ser 145 Val Val Ser Pro Asp Val Arg Ala Thr Glu 100 Ser Ala Ala Ser Ile Ala Met Leu Val 70 Thr Ile Ile Ile Gly 150 Glu Gly Set Glu Met Leu Met Ile Ala Val Thr Ala His Ile His 105 Glu Arg Ala 120 Met Leu Gly 135 Ala Asp Lys Ala Glu Gly Ala Leu Gly Val Ala Lys Gin Arg Gin Arg Ala Asp Asp Lys Val Phe Gly 110 Gin Met Set Ala 125 Ala Ala Lys Ile 140 <210> 67 <211> 1659 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1659) <400> 67 atg act gac aac agc cct cct Met Thr Asp Asn Ser Pro Pro 1 5 cgc aaa aac aac acc ttc etc Arg Lys Asn Asn Thr Phe Leu gcg ctg att gcc ggc ggc tgg Ala Leu Ile Ala Gly Gly Trp ca aac gga cac gcc caa gca cgc gtc Pro. Asn Gly His Ala Gin Ala Arg Val 10 tct gcc gtc tgg ctg gtt ccg ctg ate Ser Ala Val Trp Leu Val Pro Leu Ile 25 ctt tgg gtt aag gaa atc cgc aac agg Leu Trp Val Lys Glu Ile Arg Asn Arg 123 WO 01/85771 WO 0185772PCT/GBOI/02003 ggg oct gtg gtt Gly Pro Val Val aao aat aog gto PAsn PAn Tbr Val oga ate aaa ctg Arg Ile Lys Leu oto aat gog gao Leu Asn Ala Asp 100 gtg gto aag cog Val Val Lys Pro 115 otg ott tcg ggt Leu Leu 5cr Gly 130 gca aaa gao gtg Ala Lys Asp Val 145 ggg cas ago ggg Gly Gin 5cr Gly etc aao gto aac Leu Asn Val an 180 gto gaa ago gog Val Giu 5cr Ala 195 ato tto ato oaa Ile Phe Ile Gin 210 ttc tgg ctg gaa oto ttg atg Leu Leu.Met 55 aaa gta ttg Lys Val Leu 70 gao gao oaa Asp Asp Gin too ggo oto 5cr Gly Leu ato gao cas Ile Asp Gin 120 tao ae goe Tyr Ile Ala 135 caa gtg Cag Gin Val Gin 150 ogo ttg aat Arg Leu an cot gtt ttg Pro Val Leu tto gao oog Phe Ap Pro 200 000 eec gao Pro an Asp 215 ggo ae eat gao ago gog gaa ggo att gag gto Asp Sen Ala Glu Gly Ile Glu Val ato Ile Giy ogo Ang Gly ace Thr att Ile att Ile 170 gee Giu gao Ap otg Leu gae gto Val gaa Gin gat Asp ace Thr ggo Gly 140 coo Pro aaa Lys ttt Phe ago Ser cat His 220 aoa gga ogo Gly Arg gta aco Val Thr aco ceg Thr Gin 110 ggt ttg Gly Len 125 aaa ago Lys Ser gtt aco Vai Thr aao gac an Ap atg gte Met Val 190 gtg cat Val His 205 too goo Ser Ala ggt age gtt Val goc Ala ttt Phe ggt Gly gao Ap goc Ala ogo Arg 175 ggg Gly tao Tyr ago Scr ggC ace Thr oaa Gin tgg Trp aog Thr gag Gin ae Ile 160 ato Ile oaa Gin aoo Thr ogt Arg ae 240 288 336 384 432 480 528 576 624 672 720 Pile Trp Leu Giu Ser Gly Ile An Ile Giu Thr Thr Giy 5cr Giy Ile WO 01/85772 WO 0185772PCT/GBO1/02003 aaa oto aat too Lys Lcu Asn Ser cot Otg oct gcc otg ctg tcg ggc gcg Pro Len Pro Ala Leu Len Ser Gly Ala 250 att toe Ile 255 ttt get tog Phe Asp Set ago ttc acg Ser Phe Thr 275 aaa ace aaa aac Lys Thr Lys Aen agt aaa Set Lye 265 aec gtc aaa Aen Val Lye ago gaa gac Set Giu Asp 270 ott tao gao ago Len Tyr Asp Set ago gee gto goc aao ctg cot get Set Gin Val Ala Asn Len Pro Asp 285 gac cgt Asp Arg 290 tog ctg tao tao Ser Len Tyr Tyr gcg ttt tto aaa Ala Phe Phe Lye Ca too Gin Ser 300 gtg ogo gga Val Arg Gly acogqtc ggt tog Tb: Val Sly Set gtc gag tao aaa Val Gin Tyr Lye otg aat gtc ggo Leu Asn Val Sly 912 960 1008 gtt too gat gtt Val Set Asp Val tat tto gao ogo Tyr Phe Asp Arg gao ago otg cac Asp Set Leu His otg ttt Leu Phe 335 gaa eec ggo Gin Asn Gly gaa ate eat Gin Ile Asn 355 &tt 000 gte ogo Ile Pro Val Arg ogt att gag oot Arg Ile Gin Pro too ogt ttg 5cr Arg Leu 350 oaa oaa ttt Gin Gin Phe 1056 1104 goo gao gas Ala Asp Gin ceo ago Gin Set 360 ggo otg Sly Len 375 ace gaa oat tgg Lye Gin His Trp, cag aog Gin Thr 370 goc tta ao ea Ala Leu Asn Lye aoo goc aco Thr Ala Thr too ago eec aac Set Set Asn Aen 1152 ctg aoo ggo ago Len Tb: Sly Ser atg ett gag Met Ile Gin ttg aec gat Len Asn Asp 395 cag cot to Gin Pro Ser 1200 1248 tog ooo aeg otg Ser Pro Lye Len cog oat acc Pro His Thr gtt tat goa ggc gat Val Tyr Ala Gly Asp 410 ac gtt ae Tb: Val Ile 415 gog aoo cag Ala Tb: Gin ggo ggo ggt ttg gao gat ttg cag gto sea ttg gog gat Gly Sly Sly Len Asp Asp Len Gin Val Lye Len Ala Asp 1296 WO 01/85772 WO 0185772PCTIGBO1IO2003 ttg Ctg gao Leu Leu Asp 435 aag ttc gao aaa Lys Phe Asp Lys ct tta gat aag Pro Leu Asp Lys gtt goc gas Va. Ala Giu 1344 ttg sac Leu Asn 450 ggt tog ott gcc Gly 5er Leu Ala oto ass too aca Leu Lys 5cr Thr ass tot goc sat Lys Set Ala An goc ota ago tcc Ala Leu Sex: Sex gao ass ctg gtc Asp Lye Leu Val asa cog cag aca Lys Pro Gin Thr 1392 1440 1488 sac att ocg sac Asn Ile Pro An otg aao osa ac Leu Asn Gin Thr ass gag ttg oge Lye Giu Leu Arg aos cc Thr Thr 495 otg oss ggo Leu Gin Gly scg otg oa Tbr Leu Gin 515 tog cot oss tcg 5cr Pro Gin 5cr ato tao ggc gao Ile Tyr Gly Asp gta caa sat Val Gin An 510 coo gtc stt Pro Val Ile 1536 1584 sgt ttg gao ass 5cr Leu Asp Lys tta aaa gao gtt Leu Lye Asp Val sac act Ann Thr 530 ttg ass gas aaa Leu Lye Giu Lys ccc sac Pro An 535 gcg ctg stt Ala Leu Ile sac ago ago ago Ann Ser 5cr Sex 1632 gao oct ato cog Asp Pro Ile Pro ggs ago oga Sly Scr Arg 1659 <210> 68 <211> 553 <212> PRT <213> Neiseeria <400> 68 Met Thr Asp An meningitidis 3cr Pro Pro Pro An His Ala Gin Ala Arg Val Arg Lys Ann Aen Thr Phe Leu 8cr Val Trp Leu Vai Pro Leu Ile Ala Leu Ile Ala Gly Gly Trp Leu Trp, Val Lys Giu Ile Arg Ann Arg WO 01/85772 Glq Pro Val. Asn Asn Thr Arg Ile Ly's Leu Asn Ala Val. Val Lys 115 Len Len Ser 130 Ala Lys Asp 145 6)4' Gin Ser Leu Asn Val. Val Gin Ser 195 Ile Phe Ile 210 Phe Trp Len 225 Lys Len Asn Phe Asp Sex Phe Thr 275 PCT/GBO1/02003 Sin Gly Val Gly Giu Val Asp Tb: Thr 6)4' 125 Gly Lye 140 Pro Val Lys An Phe Met Sex Val 205 His Ser 220 Thr Gly Ser Gly Val Lys Ala Asn 285 Gin Val Val Thr Ala Gin Phe Trp 6)4' Thx Asp Gin Ala Ile 160 Axg Ile 175 Gly Gin Tyr Thr 3cr Arg Giy Ile 240 Ile Sex 255 Gin Asp Pro Asp Asp Axg Sex Len Tyr Tyr Thr Ala Phe Phe Lye Gin Ser Val Arg Gly WO 01/85772 290 Leu Thr Val Gly Ser PCT/GB01/02003 305 Val Glu Glu Gln Leu 385 Ser Ala Leu Leu Ala 465 Asn Leu Thr Asn Lys Pro 325 Ile Asp Asn Ser Arg 405 Gly Phe Leu Ser Glu 485 Ser Leu Glu Pro Pro Val 310 Tyr Phe Pro Val Glu Gin Lys Gly 375 Lys Met 390 Pro His Gly Leu Asp Lys Ala Glu 455 Ile Asp 470 Leu Asn Pro Gln Asp Lys Lys Pro 535 Lys Gly Tyr Lys Gly Leu Asn 315 Arg Asn Asp Ser Leu 330 Ile Arg Ile Glu Pro 345 Lys Glu His Trp Lys 365 Thr Ala Thr Ile Ser 380 Glu Leu Asn Asp Gln 395 Val Tyr Ala Gly Asp 410 Asp Leu Gin Val Lys 425 Pro Leu Asp Lys Thr 445 Lys Set Thr Leu Lys 460 Leu Val Gly Lys Pro 475 Thr Leu Lys Glu Leu 490 Pro Ile Tyr Gly Asp 505 Leu Lys Asp Val Gin 525 Ala Leu Ile Phe Asn 540 Arg Ser Ser Ser WO 01/85772 WO 0185772PCT/GBO1/02003 545 550 <210> 69 <211> 330 <212> DNA <213> Noessria meningitidis <220> <221> CDS <222> <400> 69 ttg cog ctg ott tgt tgt ctt tgg ttt tgg cag cot gog ga-g Lou Pro Leu Lou Cys Cys Lau Trp Phe Trp Gin Pro Ala Ala gtg aa Val Lye aag ccg ctg aag ctc ccg ctg ctg aag cac ctg cog cog Lys Pro Lou Lys Lou Pro Lou Lou Lye His Lou Pro Pro aag ctc ccg Lye LOu Pro cac ctg cog His Lou Pro cta ctg aag cac Lou Len Lys His ccg aag ctc ctg Pro Lye Lou Lou ctg cog cog aag cztc ccg ctg ctg Leu Pro Pro Lye Lou Pro Lou Lou 144 eta otg aag Leu Len Lye cac ctg cog cog aag ctc cog otg ctg His Lou Pro Pro Lye Lou Pro Lou Lou ctg cog ota cog sag cac ctg Lau Pro Lou Pro Lye His Lou cog otg sag ctg Pro Lou Lye Lou cog ota cog Pro Lou Pro cao ctg cog ctg His Lou Pro Lou aag oto ctg ctg cog Lye Leu Lou Lou Pro aag ctg caa aat aag Lye Lou Gin Asn Lye agt atc otg ott ttt 5cr Ile Lou Lou Pho 110 cat ttt His Pet cog ott goa Pro Lou Ala aag cag gat acg Lys Gin Asp Thr 330, <210> <211> 110 <212> PRT <213> Neiseria monxingitidis WO 01/85772 <400> Leu Pro Leu 1 Lys Pro Leu Leu Leu Lys Pro Lys Leu Lys Leu Pro His Leu Pro Pro Leu Ala Cys Leu Pro Leu Pro Pro Leu Lys 55 Lys His 70 Leu Leu Gin Asp <210> 71 <211> 2274 <212> DMA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(2274) <400> 71 atg aca aca tta cat ttc tca Met Thr Thr Leu His Phe Ser 1 gaa ttg aaa ttc gca caa gaa Glu Leu Lys Phe Ala Gin Glu caa gaa ttg ctg gct gtt get Gin Glu Leu Leu Ala Val Ala I cac cag gcc get gcc aac gcc His Gin Ala Ala Ala Asn Ala I rrp Leu Lys 40 His Leu Leu Thr ggc Gly aa Lys aaa Lys gat Asp 130 Leu Lys Lys Leu Leu Pro Gin Asn Leu Leu PCT/GB01/02003 Lys I Pro I Pro I Leu SLys SPhe WO 01/85772 PCTGBO1I/02003 tac gac eac ate Tyr Asp His Ile gao ctg caa gtc gee ace ggc geg att Asp Leu Gln Val Ala Thr Gly Ala Ile ccc Pro gee cgo ttc gge Ala Arg Phe Gly gac age caa aac eta tct ttg gaa can Asp ser Gin Asn Leu Ser Leu Giu Gin ttc tto Phe Phe caa etg gcg egc ggt aac aaa gac Gin Leu Ala Arg fly Asn Lys Asp 100 ttc gct ate gaa Phe Ala Ile Glu tgg ttc gao Trp Phe Asp 115 ace aac tac cac Thr Asn Tyr His ttg gtg ect gaa Len Val Pro Glu atg ace aaa Met Thr Lys 110 eac gee gat His Ala Asp ctg eaa gaa Leu Gin Glu ace gaa Thr Glu 130 ttt aaa gee &at Phe Lys Ala Asn aaa cac tac gtt Lys His Tyr Vai can cag Gin Gin 140 gee can got ttg Ala Gin Ala Leu 145 act tte etg tgg Thr Phe Leu Trp ggc ctg Gly Len 150 aaa gee aaa ccg Lys Ala Lys Pro gtt gtg ggt ccg Val Val Gly Pro ggt aaa gaa aaa Gly Lys Glu Lys gcZ gtt gaa ttc Mla Val Giu Phe gac egt Asp Arg 175 ctg age otg Leu Ser Leu get ttg gtt Ala Len Val 195 ect aaa etg ttg Pro Lys Leu Leu gtt tae gtt gaa Val Tyr Val Glu atc ctg act Ile Len Thr 190 gag ect get Glu Pro Ala gaa gee ggt gee Glu Ala Gly Mla tgg att caa ate Trp Ile Gin lie ttg act gtt gao ctg ect aaa Leu Thr Val Asp Len Pro Lys 210 215 gaa tgg gta gaa Glu Trp Vai Glu tac aaa gac gtt Tyr Lys Asp Val gao act ttg age Ala Thr Leu Ser gta agt gec aaa Val Ser Ala Lys ctg etg ago act Leu Le Ser Thr ttc ggt tat gtt gee gaa cac Phe Gly Set Val Ala Glu His gee gea ttg ttg aaa tee ctg cct gtt Ala Ala Leu Leu Lys Ser Len Pro Val WO 01/95772 PCT/GBO1/02003 gac ggc ctg Asp Gly Lou ttc gc gao Phe Ala Asp 275 cac ate gac ttg gta His Ile Asp Lou Val 260 cgc got cot gaa caa otg gat gog Arg Ala Pro Glu 265 tot goo ggo gte Ser Ala Gly Val Gin Leu Asp Ala 270 tao gao aaa gtt Tyr Asp Lye Val gao ggc cgc Asp Gly Arg aat att tgg cgc gc aac Asn Ile Trp Arg Ala Asn 290 aao aaa gtt ttg Asn Lys Val Lou act gtt gag ott Thr Val Glu Leu eaa gcc aaa otg Gin Ala Lye Lou ggo gac ogt Gly Asp Arg 310 ttg tgg att too age tot tgo Lou Txp Ile Ser Ser er.Cys 315 otg otg cac aot cog ttt gao ttg tea gtt gaa gaa aaa ctg Lou Lou His Thr Pro Phe Asp Lou Ser Val Giu Giu Lye Lou aaa gee Lye Ala 335 1008 aaO aaa cc Asn Lys Pro oaa gaa ttg Gin Glu Leu 355 otg tao tot tgg Lou Tyr 5cr Trp ttg gca Lou Ala 345 tto aco ctg Phe Thr Leu oaa aaa ace Gin Lye Thr 350 ogt got tot Arg Asp Ser 1056 cge gtc otg aaa geo gea ttg aac gaa Arg Val Lou Lys Ala Ala Lou Asn Glu 360 1104 gtt gee gao gaa ctg got Val Ala Giu Glu Lou Ala 370 age caa got got Ser Gin Ala Ala gao tee cgt go Asp Ser Arg Ala 1152 1200 ago ago gaa atc Ser 5cr Giu Ile egt goa gao gtt Arg Ala Asp Val aaa cgc ctg gco Lys Arg Lou Ala ttg cot gce aac Lou Pro Ala Asn gac caa cgo aao Asp Gin Arg Lys cca ttt gec gao Pro Pho Ala Asp ogt ato Arg Iie 415 1248 aaa geg oaa Lys Ala Gin goa tgg ttg aao Ala Trp Lou Asn cct otg etg Pro Lou Leu ccg aeg aco aao Pro Thr Thr Asn 430 1296 ate ggt tot tte cog oaa act ace gaa ate cgo caa gea cgo gca gee Ile Gly Ser Pho Pro Gin Thr Thr Glu Ile Arg Gin Ala Arg Ala Ala 1344 WO 01/85772 WO 0185772PCT/GBO1/02003 435 ttc ass ass ggo gas otg Pbs Lys Lys Gly Giu. Len 450 440 tot gcc gee gat Ser Ala Al-a Asp 455 gtt gas gag cas Val Giu Giu Gin tao gsa goc gog stg ass Tyr Giu Ala Ala Met Lys 460 1392 gsa ato gco ttg Giu Ile Ala Len gas ass Gin, Lys 475 otg gac ttg gac Leu Asp Leu Asp 480 1440 gts otg gta car- ggo gas goc gag agE Val Len Val His Gly Gin Ala Gin Arg 485 ggc gsa ttg ttg ago ggt ttt gos ttc Gly Gin Len Len Set Gly Phe Ala Phe 500 505 gse atg gtt ga Asp Met Val Gin tac tto Tyr Phe 495 1488 ace cas tat ggc Thr Gin Tyr Giy Egg gts oss Trp Val Gin 510 ggt gac gta Gly Asp Val 1536 ago tac ggt 8cr Tyr Gly 515 tct ogo Ego 5cr Arg Cys gtt ass Val Lys 520 cca cog stt ato Pro Pro Ile Ile 1584 ago cgt 3cr Arg 530 cot gas gcC atg soc gts got Egg Pro Gin Ala Met Tb: Val Ala Trp 535 tot act 5cr Thr 540 tao gcs 055 ago Tyr Ala Gin 1632 ctg aoc ass ogo cog Len Tb: Lys Mrg Pro 545 ass ggt atg ttg Lys Gly Met Leu ggo cot gts Occ Gly Pro Val Th: 1680 ctg oss tgg tot Etc gto ogo sac gat Len Gin Trp Sc: Phe Val Arg Asn Asp 565 oat ogo tot sc Pro Arg Ser Thr gtg tgt Val Cys 575 172 8 asa os ae gos Lys Gin Ile Ala 580 gos ggc ae ass Ala Gly'lie Lys 595 otg got otg aso Len Ala Leu Asn gas gta Etg gat Giu Val Leu Asp gto aec aa Val Ile Gin gao gas ct Asp Gin Pro gc Ato Ala Ile 605 otg sac Leu Asn 620 ctg gsa ass Lou Gin Lys 590 ogo gas ggc Arg Gin Gly Egg gc ggc Trp Ala Gly 1776 1824 ctg oct ttg ass ogt Len Pro Leu Lys Arg 610 gas tot Etc cgc ctg Gin 5cr Phe Mrg Len goc gat Ala Asp 615 Egg gat gcc tao Trp Asp Ala Tyr 1872 too tot ace ggt Ego gas gao ago scE ORS ato 5cr Ser Thr Giy Cys Gin Asp 8cr Thr Gin Ile 1920 WO 01/85772 625 cac aco cat His Thr His gct gca atg Ala Ala met atg gaa ctc Met Glu Leu 675 Gly Pro Gly 690 gaa gtg gag Giu Val Glu 705 ogt ctg tgg Arg Leu Trp gaa act otg Glu Thr Leu aac gao Asn Asp 650 ato gag Ile 03-u ttc &an Phe Lys cog ogo Pro Arg ate gag Ile G1U 715 ctg aaa Leu Lys 730 atg aac Met Asn ctg cot Leu Pro toa cgt 5cr Arg 670 cog sac Pro Asn 685 cog aos Pro Thr gta cog Val Pro ego ggc Arg Gly aoc cac Thr His 750 PCT/GBO1/02003 640 gog att 1968 Ala Ile 655 too gao 2016 5cr Asp gac ate 2064 Asp Ile 2112 2160 2208 2256 2274 ogt goc gaa ttg gog as Arg Ala Glu 755 Lou Ala Lys <210> 72 <211> 758 <212> PR!? <213> Neisseria meningitidis <400> 72 Met Thr Thr Leu His Phe 8cr Gly Phe Pro Arg Val Gly Al1a Phe Arg 1 5 10 Glu Leu Lys Phe Ala Gin Glu Lys Tyr Trp Arg Lys Glu Ile 5cr Glu 25 Gin Glu Lou Leu Ala Val Ala Lys Asp Lou Arg Glu Lys Asn Trp Lys 134 WO 01/85772 His Gin Ala Ala Ala Asn Ala 55 Phe Tyr Asp His lie Leu Asp 70 Ala Arg Phe Gly Phe Asp Ser Gin Leu Ala Arg Gly Asn Lys 100 Trp Phe Asp Thr Asn Tyr His 115 Thr Glu Phe Lys Ala Asn Ala 130 135 Ala Gln Ala Leu Gly Leu Lys 145 150 Thr Phe Leu Trp Val Gly Lys 165 Leu Ser Leu Leu Pro Lys Leu 180 Ala Leu Val Glu Ala Gly Ala 195 Leu Thr Val Asp Leu Pro Lys 210 215 Tyr Ala Thr Leu Ser Lys Val 225 230 Phe Gly Ser Val Ala Glu His 245 Asp Gly Leu His Ile Asp Leu 260 Phe Ala Asp Tyr Asp Lys Val 275 Asn Ile Trp Arg Ala Asn Leu PCT/GB01/02003 Asp Tyr Val Leu Gin Val Gin Asn Leu 90 Asp Gin Phe 105 Tyr Leu Val 120 Lys His Tyr Ala Lys Pro Glu Lys Gly 170 Leu Pro Val 185 Glu Trp Ile 200 Glu Trp Val Ser Ala Lys Ala Ala Leu 250 Val Arg Ala 265 Leu Ser Ala 280 Asn Lys Val 135 Ala Val Ala Thr 75 Ser Leu Ala Ile Pro Glu Val Gln 140 Thr Val 155 Ala Val Tyr Val Gin Ile Glu Ala 220 Ile Leu 235 Leu Lys Pro Glu Gly Val Gly Asp Gly Ala Glu Gln Glu Met 110 Phe His 125 Gin Leu Val Gly Glu Phe Glu Ile 190 Asp Glu 205 Tyr Lys Leu Ser Ser Leu Gin Leu 270 Ile Asp Phe Ile Phe Thr Ala Gin Pro Asp 175 Leu Pro Asp Thr Pro 255 Asp Gly Thr Pro Phe Lys Asp Glu Leu 160 Arg Thr Ala Val Tyr 240 Val Ala Arg 285 Leu Glu Thr Val Glu Leu WO 01/85772 290 Leu Gln Ala 305 Leu Leu His Asn Lys Pro Gin Glu Leu 355 Val Ala Glu 370 Asn Ser Ser 385 Leu Pro Ala Lys Ala Gin lie Gly Ser 435 Phe Lys Lys 450 Lys Glu Ile 465 Val Leu Val Gly Glu Leu Ser Tyr Gly 515 Ser Arg Pro 530 PCT/GB01/02003 295 Gly Asp 310 Phe Asp Tyr Ser Leu Lys Ala Ala 375 His Arg 390 Asp Gin Trp Leu Gin Thr Leu Ser 455 Val Val 470 Glu Ala Gly Phe Cys Val Met Thr 535 Leu Ser Leu 345 Ala Gin Asp Lys Leu 425 Glu Ala Glu Arg Phe 505 Pro Ala Ser Cys Leu Lys 335 Gln Lys 350 Arg Asp Ser Arg Leu Ala Asp Arg 415 Thr Thr 430 Arg Ala Ala Met Asp Leu Glu Tyr 495 Trp Val 510 Gly Asp Ala Gin Leu Thr Lys Arg Pro Met Lys Gly Met Leu Thr Gly Pro Val Thr Ile WO 01/85772 545 Leu Gin Trp Lys Gin Ile Ala Sly Ile 595 Leu Pro Lou 610 Giu Ser Phe
17. 625. His Tin His Ala Ala Met Met Giu Leu 675 Sly Pro Sly 690 Glu Val Gin 705 Mrg Lou Trp Gin Tin Lou Mrg Ala Gin 755 PCT/GBO1/02003 560 Cys Lys Sly Sly Ile 640 Ile Asp Ile Ala Gin 720 Lys Gin Val Met Met Asn Val Tin His Lys Len Leu Ala Lys <210> 73 <211> 2118 <212> DNA <213> Neisseria. meningitidis WO 01/85772 WO 0185772PCT/GBOIOZOO3 <220> <221> CDs <222> (2118L) <400> 73 atg ego egt ttt eta cog ate gos gcc Met Arg Arg Phe Leu Pro Ile Ala Mla tat gga ctg Tyr Gly Leu tte tgg tgg Phe Trp Trp geg gos ace Ala Ala Thr ggc age .Gly 5cr agc gea 3cr Ala 40 ata tgc gec gtc gtc ctg ttg Ile Cys Ala Val Val Leu Leu aoc agt tog etg gog gat tat Thr 5cr 3cr Leu Ala Asp Tyr atg ctg otg otg gtg ttg tec Met Leu Lcu Leu Val Leu att gtt gog tto Ie Val Ala Phe gee gtt Ala Val ttg gca cgt tat gtc ata ttg ctg ttg aaa gao agg ego gao Lou Ala Arg Tyr Val Ile lieu Lieu lieu Lys Asp Arg Arg Asp gta tto ggt tog eag att goc aaa cgo ott toe ggg atg ttt Val Phe Gly 5cr Gin Ile Ala Lys Arg Lieu 3cr Gly Met Phe aeg Thr ctg gtt gec gta ctg coo ggc gtg ttt otg ttc gge gtt tee Leu Val Ala Val Lieu Pro Gly Val Phe Leu Phe Sly Val 5cr ges cag Ala Gin ttt atc aac gge acg att aat tog Phe Ile Asn Gly Thr Ile Asn Ser 100 tte ggc aac gat Phe Gly Asn Asp ace cao gag Thr His Glu 110 aat ctg geg Asn Loeu Mla geg ctt ga Mla Loeu Glu 115 ogo agecetc aat Arg 5cr lieu Asn agc sag toe gos 5cr Lys Ser Msa goa gao Ala Asp 130 aac goCcott ggc Asn Ala Loeu Gly gee ate ec gtg Ala Ile Pro Val ata gao etc atc Ile Asp lieu Ile gcg get too ctg Mla Mla cr Lieu qgg gat stg ggc Gly Asp Met (fly sgg gtg otg gaa cat tao Arg Val Loeu Glii His Tyr 155 160 goo ggc age ggt Mla Gly 5cr Gly goc cag ott gee Mla Gin Lieu Ala tao sat gc Tyr Asn Mla goa ago ggo Ala Sc: Gly 175 WO 01/85772 aaa ate gaa Lye Ile Glu ggt aag gcg Gly Lys Aa 195 PCT/GBO1/02003 age ate aac ccg eac aag etc gat eag Ser Ile Asn Pro His Lys Lou Asp Gin 185 cog ttt cca Pro Phe Pro 190 gte agg gat Val Arg Asp egt tgg gaa aaa Arg Trp Giu Lys caa cag geg ggt Gin Gin Ala Gly ttg gaa Leu Glu 210 age ata gge gge gta ttg tac geg eag Ser Ile Gly Gly Val Leu Tyr Ala Gin 215 tgg ctg teg gca Trp Lou Ser Ala acg cae aae ggg Thr His Asn Gly gat tac gce ttg Asp Tyr Ala Leu tte cgt eag ccg Phe Arg Gin Pro Ode aaa ggc gtg gea gag gat gee gte Pro Lys Gly Vai Ala Glu Asp Ala Val 245 ate gaa aag gca Ile Glu Lys Ala agg geg Arg Ala 255 aaa tat get Lys Tyr Ala ctg gea ace Leu Ala Thr 275 ttg agt tac Leu Ser Tyr age aaa Ser Lye 265 aaa ggt ttg eag Lye Gly Lou Gin ace ttt tte Thr Pho Phe 270 otg etg att Leu Lou Ile gee tog etg ctg Ala Ser Lou Leu att ttt ott gca Ile Pho Leu Ala gte atg Val Met 290 gca ctg tat tte Ala Leu Tyr Pho cgo cgt tte gte Arg Ag Pho Val ccc gte eta tcg Pro Val Lou Ser 912 gee gag ggg geg aag gog gtg gog eaa gge gat tte age cag Ala Giu Gly Ala Lys Ala Val Ala Gin Gly Asp Phe Ser Gin cgcecC gtg ttg Arg Pro Vai Leu aac gac gag Asn Asp Glu tte ggs Phe Gly 330 cgo ttg ace aag Arg Lou Thr Lye ttg tte Leu Pho 335 1006 aae cac atg Asn His Met gag eag ett tee ate gee aaa gas gea gac gag cgo Glu Gin Leu Sor Ile Ala Lye Glu Ala Asp Glu Ag 1056 aac cgo egg ege gag gaa gee gee aga cat tat etc gas tgc gtg ttg Asn Arg Arg Arg Glu Giu Ala Ala Mrg His Tyr Lou Glu Cys Val Leu 1104 WO 01/85772 WO 0185772PCT/GB01102003 gag ggg Giu Gly 370 otg see Rag ggo Leu Thr Thr Gly gtg gtg ttt gao Val Val Phe Asp gaa cas Giu Gin 380 gge tgt ctg Gly Cys Leu 1152 aaa ace tto aac aaa gog gog gaa eag att Lys Thr ['he an Lys Ala Ala Giu Gin Ile ggg atg cog ctt Gly Met Pro Leu 1200 coo otg tgg ggc Pro Leu Trp Gly ago egg cac ggt 3cr Arg His Gly eac gge gtt tog His Gly Val 5cr gcg cag Ala Gin 415 1248 1296 cag toocotg Gin Ser Leu aog gao an Thr Asp Lys 435 ctt gee Leu Ala 420 gas gtg ttt Wax Val Pitt gcc ate ggo gog gog gca ggt Ala Ilt Gly Ala Ala Ala Gly cog gtc eat-gtg aaa tat gee gcg cog gao gat goc ass Pro Val His Val. Lys Tyr Ala Ala Pro Asp Asp Ala Lys 1344 1392 atc otg le Lau 450 otg gge aag ges sa gtc otg 000 gaa gao sac ggc aac gge Leu Gly Lys Ala Thr Val Leu Pro Giu Asp Ann Gly Asn Gly gta atg gtg att Val Met Val Ile gao gac Ap Asp 470 ate ace gtt ttg ata eac geg ea Ile Thr Val Leu Ile His Ala Gin 475 aa Lys 480 1440 1488 gaa goo gog tgg Giu Ala Ala Trp gas gtg goa asa egg etg goa eac gaa Giu Val Ala Lys Arg Leu Ala His Giu 490 sto ego Ile Arg 495 Rat cog etc an Pro Lau ttg ggo ggg Lou Giy Giy 515 c00 ato cag ott Pro Ile Gin Leu tot goo 5cr Ala 505 gsa egg otg Giu Arg Leu gog tgg asa Ala Trp Lys 510 aoa egt tog Thr Arg 1536 1584 sag etg gao gag Lys Leu Ap Giu gac gog oaa ate Ap Ala Gin Ile ace gao Thr Ap 530 aco ate ate aa Thr Ile Ile Lys gtg geg goa tta Val Ala Ala Leu gas atg gte gag Giu. Met Val Glix 1632 goa tto ego aat tae geg Ala Phe Arg PAn Tyr Ala 545 550 ogt tee cot tog Arg 3cr Pro 5cr etc aaa ttg Lau Lys Leu gsa aat eag Giu Asn Gln 560 1680 WO 01/85772 WOO1/5772PCT/GBO1/O2003 gat ttg aac gcc tta ate ggc gat gtg Asp Leu Aan Alia Leu Ile Gly Asp Val 565 ttg gca ttg tao ga& got ggt Leu Ala Leu Tyr Glu Ala Gly 570 .575 1728 cog Ego egg tEE gog gog Pro Cys AMg Phe Ala Ala gaa ott gcc Glu Leu Ala ggc Gly gaa cog ctg atg atg Giu Pro Leu Met Met 1776 geg gat aeg Mla Asp Thr 595 ace gcc atg egg Thr Ala Met Arg gtg ctg eac Val Leu His aat att Asn Ile 605 ttc aaa aat Phe Lys Asn 1824 gee gcc Ala Ala 610 gaa gcg geg gaa Glu Ala Ala Glu go gat gtg ccc gaa gtc agg gta aaa Ala Asp Val Pro Glu Val Mrg Val LYS 620 1872 gaa geg ggg cag Giu Ala Gly Gin gga egg att gtc Gly Arg Ile Val aca gtt tgc gac Thr Val Cys Asp ggc aag ggg Etc Gly Lys Gly Phe agg gaa atg etg AMg Giu Met Leu aat gee Etc gag Asn Ala Phe Giu cog tat Pro Tyr 655 1920 1968 2016 gta acg gac Val Thr Asp aaa ate atE Lys Ile Ile 675 ceg get gga acg gga ttg gga ctg ceo Pro Mla Gly Thr Gly Leu Gly Leu Pro 665 gtg gtg aaa Val Val. Lys 670 gaa gaa cac ggo Giu Glu His Gly ego atc ago ctg ago aat eag gat Arg Ile Ser Leu Ser Asn Gin Asp 685 2064 geg gge Ala Gly 690 tat geg Tyr Ala 705 gge gog tgt Gly Mla Cys gte aga Val. Mg 695 ate ate ttg eca Ile Ile Leu Pro aeg gta gaa act Thr Val. Giu Tb: 2112 2118 <210> 74 <211> 706 <212> PR? <213> Neisseria meningitidis <400> 74 WO 01/85772 Met Arg Arg PCT/GB01/02003 Phe Leu Pro Ile Ala Ala Ile Cys Ala Val Val Leu Leu 1 5 10 Tyr Phe Ala Gly Leu Phe Ala Ala Gly 145 Ala Lys Gly Leu Gly 225 Ala Val Arg Ser Leu Thr Ser Leu Leu Phe 165 Ser Trp Gly Gly Ala 245 Ala Thr Ala Phe Tyr Val Gin Ile Pro Gly Ile Asn Leu Asn Gly Asn 135 Pro Gly 150 Ala Gin Ile Asn Glu Lys Gly Val 215 Arg Asp 230 Ser Thr Ala Met Leu Leu Lys Arg Phe Leu 90 Trp Phe 105 Ser Lys Ile Pro Met Gly Ala Leu 170 His Lys 185 Gin Gin Tyr Ala Ala Leu Val Leu 250 Ser Leu Ala Asp Leu Leu Val Leu Lys Asp Arg Arg Ser Gly Met Phe Gly Val Ser Ala Asn Asp Thr His 110 Ala Leu Asn Leu 125 Gin Ile Asp Leu 140 Val Leu Glu His Asn Ala Ala Ser 175 Asp Gln Pro Phe 190 Gly Ser Val Arg 205 Gly Trp Leu Ser 220 Phe Arg Gin Pro Glu Lys Ala Arg 255 Pro Lys Gly Val Glu Asp Ala WO 01/85772 Lys Tyr Ala Leu Ala Thr 275 Val Met Ala 290 Leu Ala Glu 305 Arg Pro Val Asn His Met Asn Arg Arg 355 Glu Gly Leu 370 Lys Thr Phe 385 Pro Leu Trp Gin Ser Leu Thr Asp Lys 435 Ile Leu Leu 450 Val Val Met 465 Glu Ala Ala Asn Pro Leu PCT/GB01/02003 Leu Set Tyr Ser Lys Lys Gly Leu Gin Thr Phe Phe 265 270 ser Leu 280 Arg Arg Val Ala Glu Phe Ser Ile ,345 Ala Arg 360 Val Val Glu Gin His Gly Phe Ala 425 Lys Tyr 440 Val Leu Ile Thr Ala Lys Leu Ser 505 WO 01/85772 Leu Gly Gly 515 Thr Asp Thr 530 Ala Phe Arg 545 Asp Leu Asn Pro Cys Arg Ala Asp Thr 595 Ala Ala Glu 610 Ser Glu Ala 625 Gly Lys Gly Val Thr Asp Lys Ile Ile 675 Asp Glu Gin 520 Lys Gin Val 535 Ala Arg Ser 550 Ile Gly Asp Ala Glu Leu Met Arg Gin 600 Glu Glu Ala 615 Asp Gly Arg 630 Arg Glu Met Ala Gly Thr His Gly Gly 680 Leu Thr Arg 525 Glu Met Val Leu Glu Asn Tyr Glu Ala 575 Leu Met Met 590 Ile Phe Lys 605 Val Arg Val Val Cys Asp Phe Glu Pro 655 Pro Val Val 670 Ser ASn Gin 685 PCT/GB01/02003 Ser Glu Gin 560 Gly Ala Asn Lys Asn 640 Tyr Lys Asp Ala Gly Gly Ala Cys Val Arg Ile Ile Leu Pro 690 695 Tyr Ala 705 Thr Val Glu Thr <210> <211> 1686 <212> DNA <213> Neisseria meningitidis <220> <221> CDS WO 01/85772 <222> ()466 PCTGBO1IO2003 <400> atg tta Met Leu 1 got tta Ala Leu gog aag Ala Lys cgo tao Arg Tyr aaa atc Lys le gaa aao Gin Asn gas gat Gin Asp tgg gog Trp Ala aco gas Tin Gin 130 gaa tao Gin Tyr 145 ggo gtg Gly Val caa Gin oca Pro cog Pro ggt Gly ctc Leu Gly atg Met gog Ala 115 gao A~sp gac AXsp Sly too Set ato Ile otg Leu ttg Len ggo Gly gtg Val ogo Axg 100 atg met gao Asp ggo Gly att Ile gas sat tog ass aat tts sto tot tgt boa ata sag Gin Asn Set Lys Asa Len Ile Set Cys Set Ile Lys 5 stg Met ttt Phe sto Ile ggc Gly ogt Arg gtg Val aco Thr tac Tyr tao Tyr tco Set 165 10 tct Ser aao Asn gog Ala 55 ttg Len ggt Gly gao Asp cgt Arg ass Lys 135 gc Ala gat Asp ggo atc Gly Ile gtt aaa Val Lys ggo boa ggo Gly Set Gly oag acs gc Gin Thr Ala 75 ttg ogo oaa Leu Arg Sin 90 gta atg atg Val Met Met 105 gog att tao Ala Ile Tyr goa gas otg Ala Glu Len geg cgt gc Ala Arg Ala 155 oao sat goa His Asn Ala 170 aco Thr ttc Phe aa Lys ggo Gly gao Asp ggt Gl y gc Ala2 gaa Gin 140 goo Ala cc Tin atg oag tto ggc Met Gin Phe Gly ggo gaa ggo ao Gly Gin Gly Asn too aoo tto atg Set Tht Phe Met gas gtg gog stt Gin Val Ala Ile cag ttt gcc tao Gin Phe Ala Tyr cac soc gaa atg His Thr Gin Met 110 sat coo gas goo Asn Pro Gin Ala 125 goo aag tto gc fla Lys Phe Ala gag ttg ttg ago flu Len Len Set 160 atg gca gaa gtt 4et Ala Gin Val 175 96 144 192 240 288 336 384 432 480 528 576 gco cog ggo tto aaa ctg ogo gta ttg ctg gog ona goc ctg ttc too WO 01/85772 Ala Pro Gay sag ccg gat Lys Pro Asp 195 sat acc stc Ass Thr Ile 210 stg att atk Met Ile Ile 225 cat atg gos His Met Ala tac gao gut Tyr Asp Asp ass gac sat Lys Asp Ass 275 gtg gog Ogg Val Ala Arg 290 cgt ctg ass Arg Lou Lys 305 ccc tot acc Pro Sex Thr gcc sag Otg Ala Lys Lea ttt gas acc Phe Giu Thr 355 Lys ttg Lea tgg Trp tcg Sex ttg Lou 245 atg met sag Lys agt Sex gca Ala 055 Gin 325 cgt Arg ttg Leu Leu ctc Leu ttg Leu cac His 230 gat Asp ctc Lou gcg Ala got Ala gac Asp 310 sac Asn tag Gin ttt Phe Arg ttg Leu gas Giu 215 gac Asp tat Tyr gcC Ala aag Lys aac Asn 295 ass Lys ccg Pro gct Ala ass Lys Val gac Asp 200 ggc Gly cgc Arg sac Asn tcc Sex gas Giu 280 ass Lys atc Ile tat Tyr gtg Val sac Ass 360 PCT/1 Phe Sex gac att Asp le tct acg Sex Tin tgc acg Cys Thr 240 ggc sac Gly Ass 255 gcc ctg Ala Lou gsa ttt Glu Phe acc sgt: Thr Sax gtc ass Vai Lys 320 gas ass Giu Lys 335 asa cgc Lys Axg gcg gga Ala Gly GB01/02003 624 672 720 768 816 864 912 960 1008 1056 1104 1152 caa cgc ctc gcc atc atc ggc ccg sac ggc gcg ggc ass tcc aCC ctg WO 01/85772 G;In Arg Lou Ala 370 otg aaa oto ttg Lou Lyn Lou Lou 385 otg cog gao gaa Leu Pro Asp Giu tao tat cog caa Tyr Tyr Pro Gin 420 ago gas tgg atg 8cr Giu Trp, Met 435 ato ogo ggo act Ile Arg Gly Thr 450 ass ass gtg aag Lys Lys Val Lys 465 g ass ctg ttg Sly Lys Leu Leu aoc sac cac atg Thr Ann His Met 500 gaa asa tao sac Giu Lys Tyr Ann 515 gtt tot too ctg Val 8cr 8cr Lou 530 Ile ile Sly Pro Ann Sly Ala (fly Lys 9cr Thr 375 380 PCT/GBOI/O2003 Leu gcc ggo Ala Giy 390 ggo ago Gly 8cr 405 gat oao Asp His ogo aa Arg Gin ttg ggg Lou Sly gtt oto Val Lou 470 ctg ttg Lou Lou 485 gao atg Asp Met ggo aog Sly Thr got aco Ala Thr tao aao Tyr Ann aaa tgg Lys Trp aao gao Ann Asp 425 ggg cag Sly Gin 440 ttg oto Leu Lou ggt ggt Sly Sly acc sat Pro Asn ago ato 8cr Ile 505 att tto Ie Phe 520 ato att Ile Ile co gas Pro Giu 395 gog gaa Ala Giu 410 tto gat Phe Asp gao ggo Asp Giy tto ggo Phe Gly gaa aaa Gu Lys 475 gtc tta Val Lou 490 gas too Glu 8cr gto too Val 8cr gas ttg Glu Lou tat Tyr aaa Lys gto Val gao Asp agt Ser 460 ggc Gly gtc Vai ttg Lou 0 so His gao Asp 540 tos gac 5cr Asp goc ago Ala 8cr gat atg Asp Met 430 gao gaa Asp Giu 445 sac gat Asn Asp cgt atg Arg Met atg gat Met Asp aso atg Ass met 510 gao ogt Asp Arg 525 550 aaa 31y Lys ggt Gly gtc Val 415 gat Asp 055 Gin gto Val ott Lou gas Giu 495 gog Ala cag Gin ggc Sly otg Lou 400 ggo Gly ttg Lou gto Val gtg Val tao Tyr 480 009 Pro otg Lou tto Phe gga Sly 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 tat gas oao tao ttg ggc gat tao gaa ago tat oto gag asa ass ggo 1680 His Tyr Lou Asp Tyr Glu 8cr Lou Giu LYS Lys 1686 WO 01/85772 Val. Ala <210> 76 <211> 562 <212> PRT <213> Neisseria PCTIGBO1/02003 meringititi s <400> 76 Met Leu C 1 Ala Leu I Ala Lays I Arg Tyr C Lys Ile I Glu AsnC Glu Asp b% Trp Ala Thr Giu 130 Glu Tyr 145 Gly Val. C Ala Pro C Lays Pro Aon 8cr L~ys Ile Bar Thr Glu Asn Val Gly Ala Asn Asp Leu Glu Leu Gly Lays Leu Asp Val Glu Arg Asp 120 Met Lays Ala 135 Thr Ala Giu 150 Giu Asp Leu Leu Arg Val Leu Leu Asp 200 WO 01/85772 Ann Thr1le Arg PCT/GBOJ/02003 Trp Lou Giu Gly Val Lou Ann Gin Tyr Asp 5cr Thr 210 215 220 Met Ile 225 His Met Tyr Asp Lys Asp Val Ala 290 Arg Lou 305 Pro 5cr Ala Lys Phe Giu Gin Mrg 370 Leu Lys 385 Lou Pro Tyr Tyr Ciu Ile Axg 450 Ile Asp Tyr 260 Ala Phe Gin Arg His 340 Gin Ala Lou Giu Gin 420 Met Thr Asp Tyr Ala Lys Anni 295 Lys pro Ala Lys Gly 375 Ala Ie Giu Trp AMg 455 Mxg His Asn Thr 5cr Ala 265 Giu Lys 280 Lys 8cr Ile Lys Tyr Ile Val Glu 345 Ann Lou 360 Pro An Tyr An Lys Trp Ann Asp 425 G1y Gin 440 Len Lou An Ile Mrg Giu Arg 300 met Giu Lys Ile Gly 380 Tyr Lys Val Asp 460 WO 01/85772 Lys Lys val 465 Gly Lys Leu Thr Asn His Glu Lys Tyr 515 Val Ser Ser 530 Tyr Glu His 545 Val Ala Gly Gly Pro Asn Ser Ile 505 Ile Phe 520 lie Ile Tyr Glu <210> 77 <211> 1773 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1773) <400> 77 atg agt atc gtg ctg cac ggc gtg gcg Met Ser Ile Val Leu His Gly Val Ala 1 5 ggt tgc gcc cac ctg att gcg cgc ggt Gly Cys Ala His Leu Ile Ala Arg Gly 25 gat gtt gcg gag gcg gac acc gat gec Asp Val Ala Glu Ala Asp Thr Asp Ala 40 gcc gtc aaa gcc acg cgc aaa gag ttg Ala Val Lys Ala Thr Arg Lys Glu Leu 55 150 Glu Val 490 Glu Val Glu Ser gcg Ala 10 acg Thr gaa Glu gaa Glu Lys Gly 475 Leu Val Ser Leu Ser His Leu Asp 540 Tyr Leu 555 Met Asp Met 510 Arg Lys Lys att lle ccg Pro ttc Phe agc Ser Leu Glu 495 Ala Gin Gly Lys gcc Ala cag Gin gat Asp gcg Ala PCT/GB01/02003 Tyr 480 Pro Leu Phe Gly Gly 560 gtc 48 Val tat 96 Tyr gcc 144 Ala att 192 Ile aaa gge Lys Gly gaa gtg Glu Val gaa cgt Glu Arg ctc cgc Leu Arg WO 01/85772 PCT/GBO1/02003 ccc gas sac gc Pro Glu Asn Ala cag acc gag ttg ggc gcg ttc ate tcg ctg ca0 Pro Thr Glu Leu Gly Ala Phe Ile Ser Leu His otc ttg acc Leu Leu Thr gat gtt acc ttg tcg Asp Val Thr Leu Ser gaa ccc gto gat Glu Pro Val Asp att tts Ile Leu agg gas caa Arg Glu Gin stu aac gco gag Ile Ann Ala Glu gca ttg sag Ala Leu Lys cag cag agc gac Gin Gin Ser Asp 110 aaa otc gao goc caa tta gao Lys Leu Ala Ala Gin Phe Asp 115 atg gac gat gco Met Asp Asp Ala ttg cgc gas Leu Arg Glu oge nag Arg Lys 130 cag gat atg ctg caa gtc gte cgo cgc Gin Asp Met Lou Gin Val Val Arg Arg 135 cac aac aac otg His Ann Asn Leu ggg cag ggc aac Gly Gin Gly Asn ttg gas gtt goc Leu Giu Val Ala gac aac ctg ttt Asp Ass Leu Phe 155 gcc gac acg gtt Ala Asp Thr Val gac Asp aco gtt ctg att Thr Val Leu Ile aac gac ctt tog Asn Asp Leu 5cr ttg ttt Len Phe aaa gag Lys Glu cag cgc att Gin Arg Ile gcc gcc ttc gtt ace gat gc ggc Ala Ala Phe Val Thr Asp Ala Gly 185 175 ggo cce acc Gly Pro Thr 190 tcc gte gte Ser Val Val ggg cat acg Gly His Thr 195 gcg att ttg ggc Ala Ile Lou Gly agc ttg gac atc Ser Leu Asp Ile ggg ctg cac aac gcg ego Gly Leu His Ann Ala Arg aaa ctg att acc Lys Leu Ile Thr gag ggc gaa acg gte att Gin Gly Giu Thr Val Ile 220 gcg cog gat gag tog gtg Ala Pro Asp Glu Ser Val 235 240 gac ggt atc aac ggo gtg ttg att ate Asp Gly Ile Ann Gly Vai Leu Ile Ile 230 ttg aao Len Asn gaa tac cgc cgc cgt gco cgc Giu Tyr Arg Arg Arg Ala Arg 245 tao cgo agc Tyr Arg Ser cac aaa cgo His Lys Arg 255 WO 01/85772 WO 0185772PCT/GBOI/02003 gat ttg eec eag etc aaa ace ace gcc gee gcc ace get gee ggg gtc Asp Leu Asn Lys Leu Lys Lys Thr Ala Ala Ala Tb: Ala Asp Gly Val 270 tge ate gag ye Ile Giu 275 ett gtg gge Leu Val Gly eat ate gaa Asn le Giu tee gcc gaa gae Ser Ala Giu Asp 285 gtg aaa ceg Val Lys Pro ctg ec Leu His 290 aacectc gge gee Asn Leu Gly Ala gge ate ggg etg Gly Ile Gly Leu ege age gag ttt Arg Ser Giu Phe tac ctg aec cgc Tyr Leu Asn Arg aeg atg ceg tct Thr Met Pro Ser gac gag cag tee Asp Giu Gin TYZ' gtg tac age gcg att gte aaa aaa atg Val Tyr Ser Ala Ile Val Lys Lys Met 325 gge aec age gta Gly Lys 5cr Val aeg eta Tb: Ile 335 1008 egg ace gte Ar; Tb: Val aae ago aeg Asn Se: Thr 355 ttg ggt gtg gac Leu Gly Val. Asp aac ceg ege tgg Asn Pro Ar; Trp tc ggg aaa Phe Gly Lys 350 atg ace ggc Met Tb: Gly 1056 1104 eec aae ggc age Pro Asn fly 5cr aec ccc geg etg Ann Pro Ala Leu ate ege Ile Mrg 370 etg tgCcett gee Leu Cys 15cc Ala eeg gte atg tte Pro Val Met Phe ace cag at; ege Thr Gin Met Ar; 1152 1200 ate etc cgc gee Ile 15cc Arg Ala gee eec gge coc Ala His Gly Pro egg etg etg tgg Arg Met Met Trp, at; att ace tee Met Ile Tb: 5cr ace gcg cee ege Thr Ala Gin Mrg 420 tee gaa gtg ege eag tge etc ate eac Sc: flu Val Mrg Gin Cyn 15c Ile His 410 etc gae Leu Asp 415 1248 czag ott gee gaa ege ggc gat gee tte Gin Leu Ala Gic Ar; Gly Asp Ala Phe 425 ggt aaa gte Giy Lys Val 430 ae gtc gge Th: Val fly 1296 ggc ate gge tgt atg att gaa Gly Ile Gly Cys Met Ile Giu 435 att ccg Xie Pro 440 tet gee geg 5cr Ala Ala 1344 WO 01/85772 WO 0185772PCT/GBO1/02003 agt att Se: Ile 450 ttg aaa ctg gte gat ttc ate tee gto ggt ace sac ga ctg Leu Lys Leu Val Asp Phe Ile 5cr Val Gly Thr Asn Asp Lou 1392 eaa tac ate ttg tee gtc gat ege Gin Tyr Ile Leu 5cr Val Asp Arg 470 ggc ga gac age gte Gly Asp 4.75 Asp 5cr Vai age eac 5cr His 480 eac gte His Val 495 1440 1488 etc tac eag eCC gge cat eec gcc gtg etg aaa atg etg cas Leu Tyr Gin Pro Gly His Pro Ala Val Lou Lys Met Lou Gin ate cgt ace Ile Arg Thr atg geg ggc Met Ala Gly 515 gee aac Ala Aan 500 egc atg gac aaa gac gta tee gta Arg Met Asp Lys Asp Val Se: Val 505 tge gge gag Cys Gly Giu 510 atg ggg etg Met ely Leu 1536 gat ace gag ttt Asp Thr Ala Phe ego- gtt tta ttg Arg Val Leu Lou 1584 ege egt ttt tee aig aac Arg Arg Phe 5cr Met Asn 530 sac aac ate ctg ce gte aaa aae ate Asn Asn Ile Leu Pro Val Lys Asn Ile 1632 1680 etg eac age sat gte gga eag ate gas Lou His Sex Ass Val Gly Gin Lou Glu 550 gat atE gtg aaa Asp Ile Val Lys ate age tge gaa Ile Mrg cys Gin ga gaa Asp Glu 565 gag aag teg Glu Lys 5cr sag ctg ate aaa Lys Lou Ile Lys eag atg Gin Met 575 1728 aae age gtg Ass 3cr Val gtc gag gsa gaa gee ga tte aag Val Gin Gin Gin Ala Asp Pho Lys 585 ggg egg aaa Gly Arg Lys 590 1773 <210> 78 <211> 591 <212> PRT <213> Noisseria meningitidis <400> 78 Met 5cr Ile Vol Lou His Gly Val Ala Ala Gly Lys Gly Ile Ala Vat Gly Cys Ala His Lou Ile Ala Mxg Gly Thr Gin Gin Val Pro Gin Tyr WO 01/85772 PCT/GB01/02003 25 Asp Val Ala Glu Ala Asp Thr Asp Ala Glu Ala Glu Arg Phe Asp Ala 40 Ala Val Lys Ala Thr Arg Lys Glu Leu Glu Gln Leu Arg Ser Ala Ile 55 Pro Glu Asn Ala Pro Thr Glu Leu Gly Ala Phe Ile Ser Leu His Leu 70 75 Met Leu Leu Thr Asp Val Thr Leu Ser Arg Glu Pro Val Asp Ile Leu 90 Arg Glu Gin Lys Ile Asn Ala Glu Trp Ala Leu Lys Gin Gin Ser Asp 100 105 110 Lys Leu Ala Ala Gin Phe Asp Asn Met Asp Asp Ala Tyr Leu Arg Glu 115 120 125 Arg Lys Gin Asp Met Leu Gin Val Val Arg Arg le His Asn Asn Leu 130 135 140 Ile Gly Gin Gly Asn Glu Leu Glu Val Ala Asp Asn Leu Phe Asp Glu 145 150 155 160 Thr Val Leu Ile Ala Asn Asp Leu Ser Pro Ala Asp Thr Val Leu Phe 165 170 175 Lys Glu Gin Arg Ile Ala Ala Phe Val Thr Asp Ala Gly Gly Pro Thr 180 185 190 Gly His Thr Ala Ile Leu Gly Arg Ser Leu Asp Ile Pro Ser Val Val 195 200 205 Gly Leu His Asn Ala Arg Lys Leu Ile Thr Glu Gly Glu Thr Val Ile 210 215 220 Val Asp Gly Ile Asn Gly Val Leu Ile Ile Ala Pro Asp Glu Ser Val 225 230 235 240 Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg 245 250 255 Asp Leu Asn Lys Leu Lys Lys Thr Ala Ala Ala Thr Ala Asp Gly Val 260 265 270 Cys Ile Glu Leu Val Gly Asn Ile Glu Ser Ala Glu Asp Val Lys Pro 154 WO 01/85772 275 Leu His Asn Leu Gly 290 Leu Tyr Leu Asn Arg 305 Val Tyr Ser Ala Ile 325 Arg Thr Val Asp Leu 340 Asn Ser Thr Pro Asn 355 Ile Arg Leu Cys Leu 370 Ala Ile Leu Arg Ala 385 Met Ile Thr Ser Val 405 Thr Ala Gin Arg Gin 420 Gly Ile Gly Cys Met 435 Ser Ile Leu Lys Leu 450 Ile Gin Tyr Ile Leu 465 Leu Tyr Gin Pro Gly 485 Ile Arg Thr Ala Asn 500 Met Ala Gly Asp Thr 515 PCT/GB01/02003 Ala Asp 310 Val Gly Gly Ala Ala 390 Ser Leu Ile Val Ser 470 His Arg Ala Asp Gly Ile 295 Thr Met Pro Lys Lys Met Val Asp Lys 345 Ser Leu Asn 360 Glu Pro Val 375 Ala His Gly Glu Val Arg Ala Glu Arg 425 Glu Ile Pro 440 Asp Phe Ile 455 Val Asp Arg Pro Ala Val Met Asp Lys 505 Phe Thr Arg 520 Gly Ser Lys 330 Asn Pro Met Pro Gln 410 Gly Ser Ser Gly Leu 490 Asp Val Leu Glu 315 Gly Pro Ala Phe Val 395 Cys Asp Ala Val Asp 475 Lys Val Leu Phe 300 Asp Lys Arg Leu Arg 380 Arg Leu Ala Ala Gly 460 Asp Met Se Leu Arg Ser Glu Gin Ser Val Trp Phe 350 Gly Met 365 Thr Gin Met Met Ile His Phe Gly 430 Leu Thr 445 Thr Asn Ser Val Leu Gn Val cys 510 Gly Met 525 Glu Tyr Thr 335 Gly Thr Met Trp Leu 415 Lys Val Asp Ser His 495 Gly Gly Phe Glu 320 lie Lys Gly Arg Pro 400 Asp Val Gly Leu His 480 Val Glu Leu Arg Arg Phe Ser Met Asn Pro Asn Asn Ile Leu Pro Val Lys Asn Ile WO 01/85772 PCTIGBO1/02003 530 535 540 Ile Leu His Ser Msn Val Gly Gin Leu Giu Se: Asp Ile Val Ly's Val 545 550 555 560 Ile Mrg Cys Giu Asp Giu Glu Lys Ser Giu Lys Leu Ile Lys Gin Met 565 570 575 Asn 5cr Val Ser Val Glu Giu Giu Aia Asp Phe Lys Gly AMg Lys 580 585 590 <210> 79 <211> 1062 <212> DNA <213> Neisseria meningitidis <220> <221> 005 <222> (1)..(1062) <400> 79 &tg gaa ass gaa t Met Giu Lys Giu P, 1 ggt gga ggc gas. c, Giy Gly Giy Glu G, cgg gac tgc aca a Arg Asp Cys Tb: Mi cac agg ega ttt ti His Arg Arg Pbs Si acg etc sac ggg ci Tb: Leu Asn Gly Li ass sac cgc stt to Lys Asn Axg Ile Si tta too ata ott. ti sgg ata tts sat ate gta tcg goc sag att tgg Arg Ile Leu As Ile Vai Se: Ala Lys Ile Trp 10 ass gca Lys Ala sat. sat Asn Ass ag sea Tb: Thr aecegc Tbr Arg ggc aaa Gly Lye ego ctg WO 01/95772 Leu Sex Ile PCTIGDOIIO200J Leu Lieu Lys Lys Leu Tin Gly Val Arg Leu Ile [The Val 100 110 aa Lys cag Gin gtg Vai 145 aac Asn agc 3cr gga Gly cct Pro tgt Cys 225 tot 8cr gat Asp tta Lieu gtt gto Vai Val acc gao Thr Asp goc gao Ala Asp gat ace Asp Thr 165 ttt acc Phe Thr 180 aac ctg Asn Lieu agg eto Arg Lieu aag cgg Lys Arg ggg ttt Gly Phe 245 gtt ttg Val Lieu 260 gog atg Ala Met gaa aaa Giu Lys 155 ccc cct Pro Pro 170 ggc agg Gly Arg gtg ata Val Ie gac gga Asp Giy tog gga 8cr Giy 235 got tcg Ala 5cr 250 ccg gag Pro Giu gog gtg Ala Val tat Tyr Cgt Arg 140 tao Tyr Gin ate Ile ctg Lieu cat His 220 goa Ala ttt Phe gca Ala att Ile cac ogo ctg ata His Arg Lieu Ile 125 ctg gtt tao gat Lieu Val Tvr Asn gaa cca Giu Pro tao ogo Tyr Arg ttc ggt Phe Gly 270 too aat Sex Asn 285 ggo gog osa aag gaa att ato gas cat cat oaa tog ggg att ctg otg WO 01/85772 Gly Ala Gin Lys 290 gat agg ctg aca Asp Arg Leu Thr 305 tta aac cct gaa Leu Asn Pro Glu gtc gcc aac cgt Val Ala Asn Arg 340 gea ata Ala Ile <210> <211> 354 <212> PRT <213> Neisseria <400> Met Glu Lys Glu 1 Gly Gly Gly Glu Arg Asp Cys Thr His Arg Arg Phe Thr Leu Asn Gly Lys Asn Arg Ile Leu Ser Ile Leu 100 Lys His Asn Val 115 Gly lie gaa cge Glu Arg gga cat Gly His aaa tta Lys Leu 350 PCT/GB01/02003 Leu gte 960 SVal 320 tgc 1008 Cys gat 1056 SAsp 1062 meningitidis Phe Arg Ile Leu Asn Gn Tyr Val Tyr Asp Met Phe Thr Ala Val Ser Glu Val Ser Ser Leu Phe Ser Leu Tyr Ser His Leu Met Ile Leu Lys Lys Leu Thr 105 Val Ala Asn Lys Thr 120 158 WO 01/85772 PCT/GB01/02003 Gin Lys Asn Thr Asp Arg Phe Ile Cys Val Ser Arg Leu Val Tyr Asp 130 135 Val 145 Asn Set Gly Pro Cys 225 Ser Asp Leu Gly Asp 305 Leu Val Ala Gin Thr Gly Ile Arg Phe Leu Glu 195 Gin lie 210 Arg Leu Phe Glu Val Val Cys Glu 275 Ala Gin 290 Arg Leu Asn Pro Ala Asn Ile Ala Asp Phe 180 Asn Arg Lys Gly Val 260 Ala Lys Thr Glu Arg 340 Phe Lys Phe Pro Tyr Ala 185 Ala Cys 200 Ala Gly Ser Ala Lys Leu Leu Val 265 Arg Thr 280 Glu His Leu Ala Ala Leu Asn His 345 Glu Pro 170 Gly Val Asp Ser Ala 250 Pro Ala His Asp Ala 330 Thr 140 Tyr Arg Ile Gln Glu Lys Ile Set Pro 190 Leu His Arg 205 His Pro Asp 220 Ala Glu Pro Phe Tyr Arg Ala Phe Gly 270 Ile Ser Asn 285 Ser Gly Ile 300 Ile Glu Arg Ala Gly His Asp Lys Leu 350 Val His 160 Pro Asp 175 Glu Lys Lys Tyr Tyr Met Phe Val 240 Gin Ser 255 Leu Ser Thr Leu Leu Leu Leu Val 320 Gin Cys 335 Leu Asp WO 01/85772 PCT/GBO1/02003 <21.0> 81. <211> '795 <212> DNA <213>' Neisseria meningitictis <220> <221> ODS <222> (795) <400> 81 atg gag got gtt ttt tot gta tgt gco tgt ttt atg tgt tac tgc ott 48 Met Ala Ala Val Phe 5er Val Cys Ala Cys Rho Mot Cys Ser Cys Lou 1 5 10 gtt gta aaa aat aca gtt ata ggs ago gga ago aoc aaa atc gao gtg 96 Val Val Lys Asn Thr Val le Gly So: Gly Arg Tb: Lys Ile Ala Val 25 cog ott gta gcc ago gat go gao gta ott too gao gta ctt gac oaa 144 Pro Lou Val Ala Arg Asp Ala Ala Val Leu Ser Ala Val Lou Asp Gin 40 ata aaa aaa ctg coo tto gat ata gta gag ttc ago gao gat ttt ttg 192 Ile Lys Asn Leu Pro Phe Asp Ile Val Gin Phe Arg Ala Asp Pho Lou 55 gaa tgo gag ggo ago ata gga gaa gta ttg ago coo aog cag aoa gta 240 Giu Cys Ala 013? Scr Ie Gly Gin Val Lou Arg His Thr Gin Thr Val 70 75 ago gao gag ota aaa gao aag cog otg otg ttt aag tta ago agg oat 288 Arg Asp Ala Leu Pro Asp Lys Pro Lou Leu Pho Thr Pho Arg Arg His 90 gga gaa gga ggo tog tto oog tgt tag gao gat tat tat ttt gaa atg 336 Gly Giu Gly Gly Ser Phe Pro Cys So: Asp Asp Tyr Tyr Phe Giu Lou 100 105 110 ato gao gag otg sta gaa ago ago otg ooo gao ato ata gao sta gag 384 Leu Asp Ala Le Ile -Giu 5cr Arg Lou Pro Asp Ile Ile Asp Ile Giu 115 120 125 atg ttt too ggo gas aa gao gto ogg tgo gao gtg goa aat got aaa 432 Len Rho Set Gly Gin Thr Ala Val Arg Cys Ala Val Ala Asn Ala Gin 130 135 140 ass sac gga ata gao goo otg cta tgo sat oat gag ttt ao ago ag Lys Asn Gly Ile Ala Ala Lou Len Cys Asn His Giu Pho His Arg Thr WO 01/85772 145 ccg ccg caa gaa Pro Pro Gln Glu gge gcg gac ate Gly Ala Asp Ile 180 gtg ctg act ttg Val Leu Thr Leu 195 aaa ccg att gtt Lys Pro Ile Val 210 ctt gcc gga cag Leu Ala Gly Gln 225 caa aac tcc gcg Gln Asn Ser Ala ctc gac tgc ctc Leu Asp Cys Leu 260 <210> 82 <211> 265 <212> PRT <213> Neisseria <400> 82 Met Ala Ala Val 1 Val Val Lys Asn Pro Leu Val Ala Ile Lys Asn Leu 150 atc gta Ile Val aaa att Lys Ile tee gcc Ser Ala atg tcg Met Ser 215 ttc ggc Phe Gly 230 ggg caa Gly Gin aac gga Asn Gly cgt ctg Arg Leu 170 gtg atg Val Met 185 etc aaa Leu Lys ggg cag Gly Gin age ate Ser Ile ggc gta Gly Val 250 gac Asp 265 cag atg gag Gin Met Glu age gcg Set Ala 190 gag ctt Glu Leu 205 gcg gtc Ala Val ggt tcg Gly Ser ctc cgt Leu Arg PCT/GB01/02003 160 gac tgc 528 Asp Cys 175 gaa gat 576 Glu Asp gee gcc 624 Ala Ala age egg 672 Ser Arg gga acg 720 Gly Thr 240 gcg aca 768 Ala Thr 255 meningitidis Phe Ser Val 5 Thr Val Ile Arg Asp Ala Pro Phe Asp 55 Cys Gly Ala 40 Ile 161 Ala Cys Phe Met Cys Ser Cys Leu 10 Ser Gly Arg Thr Lys Ile Ala Val 25 Val Leu Ser Ala Val Leu Asp Gln Val Glu Phe Arg Ala Asp Phe Leu WO 01/85772 Glu Cys Ala Arg Asp Ala Gly Glu Gly Leu Asp Ala 115 Leu Phe Ser 130 Lys Asn Gly 145 Pro Pro Gin Gly Ala Asp Val Leu Thr 195 Lys Pro Ile 210 Leu Ala Gly 225 Gin Asn Ser Leu Asp Cys PCT/GB01/02003 Thr Val Arg His Glu Leu Ile Glu Ala Gin Arg Thr 160 Asp Cys 175 Glu Asp Ala Ala Ser Arg Gly Thr 240 Ala Thr 255 Gly Val 250 Asp 265 Ser Ala Leu Arg <210> 83 <211> 876 <212> DNA <213> Neisseria meningitidis <220> WO 01/85772 PCT/GBO1/02003 <221> ODS <222> <400> 83 ttg acc gtc oga aog aaa aag aog gcg oat tat ace ota ttc cat too 48 Leu Thr Val Arg Thr Lys Lys Tbr Ala His Tyr Thr Leu Phe His 1. 5 10 gao oga aaa ccg aao atg act act otc ass ccc gcc ctg ccc got tat 96 Asp Arg Lys Pro Asn Met Thr Th: Len Lys Pro Ala Len Pro Ala Tyr 25 ctg gao sac ato cgc atc atc etc aeg ogo acc ago cat coo goc sac 144 Leu Asp As Ile Arg Ile Ile Len Tb: Arg Th: 8cr His Pro Ala Asn 40 ate ggo tot goc gcg cgc gcg atg ass aca atg ggt otg oac ass ctg 192 Ile Gly 8cr Ala Ala Arg Ala Met Lys Tbr Met Gly Len His Lys Leu 55 aco atc gtc goc aca aat ctg atg gca acg cog atg acg gas aac cog 240 Thr Ile Val Ala Pro Asn Len Met Ala Thr Pro Met Tb: Gin Asn Pro 70 75 ccc gtg ttt gao cog gag cat oct osa tcg ttt aaa tta cog gaa gaa 288 Pro Val Phe Asp Pro Gin His Pro Gin 5cr Phe Lys Leu Pro Glu Gin 90 ago tto atc otc got too ggc gog gos gao gtt ttg gasasat goc ac 336 Sc: Phe Ile Leu Ala 5cr Gly Ala Ala Asp Val Len Gin Asn Ala Th: 100 105 110 att goc got tot ttg gao gas goecott goc gao aco aco ato goe tgc 384 Ile Ala Ala 5cr Len Asp Gin Ala Len Ala Asp Tb: T: Ile Ala Cys 115 120 125 goc otg aco ago ogo ogo ogo gs att act gog cog ctg caa aco cog 432 Ala Len Tb: 5cr Asg Arg Arg Gin Ile Tb: Ala Pro Len Gin Tbr Pro 130 135 140 ogo gat ttg gta too gas tta otg cag aoc gos aso oga ggc gag aaa 480 Arg Asp Len Val Ser Gin Len Len Gli Tb: Ala Asn Arg Gly Gin Lys 145 150 155 160 gtg goa ctg gtt tto ggc aac gag act ttc ggc ttg agc ato gaa gsa 528 Val Ala Len Val Phe Gly Asn Gin Thr Phe Gly Len 8cr Ile Gin Gin 165 170 175 WO 01/85772 gtc eta gee Val Gin Ala ttc teg ctc Phe 3cr Lou 195 PCT/GBO1/02003 tge aac ega etg atg ace ate ate gge' aat eec gac tat Cys Asn AMg Leu Met Thr Ile Asn Gly Ann Pro Asp Tyr 180 185 190 tac ctc gee eat gcc gtg cag gtc gtg tgc tac gat ate Asn Leu Ala Gin Ala Val Gin Val Val Cys Tyr Giu Ile .624 ttc agc Phe 5cr 210 get geg Ala Ala 225 eat ace ggt teg Gin Thr Gly 5cr atg ace cat ett Met Thr His Lou eaa gat gac ec Gin Giu Asp His acc etc gag Thr His Giu ate aaa ggc atg Ile Lys Gly Met gte gcc Vai Ala 235 etc atg gaa His met Giu gtg atg aae gte Val Met Ann Asp gge ttt tte ate ege ege aae gge gag Gly Phe Phe Asn Mrg Arg Asn Gly Glu 250 cgt etg Arg. Leu 255 atg ege egt Met Mrg Arg gte ate gat Asp Ile Asp 275 eag age etg tte Gin Ser Lou Phe gga egc Giy Mrg 265 gee ate aeg Ala Ann Thr cat ace gaa Gin Thr Giu 270 cat cgt ate His Mrg Ile ate ctg egc ggt Ile Leu Mrg Gly tte aat tee gte Phe Asn Thr Val eat aaa at gte His Lys Lys Asp 290 <210> 84 <211> 292 <212> PRT <213> Neisserit <400> 84 Lou flit Val Mrg 1 Asp Arg Lys Pro Leu Asp Asn Ile meningitidis Thr Lys Lys Thr Ala His Tyr Thr Lou Phe His 8cr Ann Met Thr Thr Lou 25 Lys Pro Ala Lou Pro Aia Tyr Pro Ala An Mg Ile Ile Leu Thr Arg Thr 5cr WO 01/85772 PCT/GB01/02003 Ile Gly Ser Ala Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 55 Thr Ile Val Ala Pro Asn Leu Met Ala Thr Pro Met Thr Glu Asn Pro 70 75 Pro Val Phe Asp Pro Glu His Pro Gin Ser Phe Lys Leu Pro Glu Glu 90 Ser Phe Ile Leu Ala Ser Gly Ala Ala Asp Val Leu Glu Asn Ala Thr 100 105 110 Ile Ala Ala Ser Leu Asp Glu Ala Leu Ala Asp Thr Thr Ile Ala Cys 115 120 125 Ala Leu Thr Ser Arg Arg Arg Glu Ile Thr Ala Pro Leu Gin Thr Pro 130 135 140 Arg Asp Leu Val Ser Glu Leu Leu Gin Thr Ala Asn Arg Gly Glu Lys 145 150 155 160 Val Ala Leu Val Phe Gly Asn Glu Thr Phe Gly Leu Set Ile Glu Glu 165 170 175 Val Gin Ala Cys Asn Arg Leu Met Thr Ile Asn Gly Asn Pro Asp Tyr 180 185 190 Phe Ser Leu Asn Leu Ala Gin Ala Val Gin Val Val Cys Tyr Glu lie 195 200 205 Phe Ser Gin Thr Gly Ser Pro Met Thr His Leu Gin Gin Glu Asp His 210 215 220 Ala Ala Thr His Glu Gin Ile Lys Gly Met Val Ala His Met Glu Ser 225 230 235 240 Val Met Asn Asp Ile Gly Phe Phe Asn Arg Arg Asn Gly Glu Arg Leu 245 250 255 Met Arg Arg Met Gin Ser Leu Phe Gly Arg Ala Asn Thr Gin Thr Glu 260 265 270 Asp Ile Asp Ile Leu Arg Gly Phe Phe Asn Thr Val Ser His Arg Ile 275 280 285 His Lys Lys Asp 290 WO 01/85772 WO 0185772PCT/GBOI/02003 <210> <211> 1545 <212> DNA <213> Neisseria meningitidis <220> <221> ODS <222> <400> gtg ogt oto aat cat ttc ata atg ate gag att, att ata tat gtg att Val Arg Lou Asn His Phe ile Met Ile Ala Ile Ile Ile Tyr Val Ile tcc cct gca Pro Ala gct ttg cct Ala Lou Pro aag cog gcc cgc cgc cac agc gtt coo Lys Pro Ala Arg Arg His 5cr Val Pro act tat cog Thr Tyr Pro tat aat tgc ttt Tyr Asn Cys Phe tat gta aca gat tta cot &tg eat Tyr Val Thr Asp Leu Pro Met Asn ttc ccc ace aca gag gcc too etg otg etg ott cto gcc tce ctc gcc Phe Pro Lys Thr Ala Ala 5cr Lieu Lou Lou lieu Lou Ala 5cr Lou Ala goa cac gog ctc gat aca ggt cgc att ccg Ala His Ala Leu Asp Thr Gly Arg Ile Pro caa aao gee etc gc Gin Asn Giu Ile Ala tat gtc cac gag Tyr Vel Gin Giu gao ago gge cee Asp 5cr Gly Lys atc att gac cc Ile Ile Asp His cgo tag Arg 8cr gat gte ccc Asp Val Pro ac ccc goc tea Asn Pro Ale 5cr atg ace etc gtt ccc gog ttt Met-Lys lieu Val Thr Ala Pho 110 gac goc ttc aaa acc tto ggc ago aat tao ogc Ala Ala P1-t Lys Thr Phe Gly 3cr Ann Tyr Arg tgg gog Trp Ala 125 acc gag ttt Thr Giu Phe aaa agc aao Lys 5cr Asn 130 ggt ag gta Gly Thr Val gee ggc acg ott gao gga aaa ctg tat Asp Gly Thr Lieu Asp Sly Ann Leu Tyr 140 WO 01/85772 WO 0185772PCT/GBOI/02003 gag ggo ago ggo gac ccc gtt tto aat cag gaa aat ctg ctt gaa Ala Gly Set Gly Asp Pro Val Phe Asn Gin Giu Asn Leu Leu Ala gto one aga cag ttg ago gaa aa Val Gin Arg Gin Leu Arg Giu Gin 165 ggo eta Gly Ile 170 ago eat ata ag Arg Asn Ile Thr gge cac Gly His 175 ctg etg ata Leu Met Leu gac ceo ago ctg tgg ggc gee gtc ggc aga ccc gec get Asp His Ser Leu Trp Giy Gin Val Giy 5cr Pro Asp Asp tta gee goo gao agc ggt tog cog ttt atg ag ccc Ph. Giu Ala Asp 5cr Gly 5cr Pro Phe Met Thr Pro eat ca act Asn Pro Thr atg ctg tat gao ggt atg Met Leu 5cr Ala Giy Met atg gtg cga gao Met Vai Arg Ala cgcaeat gao gc Arg Asn Ala Ale agt ea gao atc 8cr Thr Asp Ile nov get cog act Thr Asp Pro Pro cog at att ttc Pro His Ile Phe can eec aac ttg eaa ett aaa goc tc Gin Asn Asn Leu Lys Ile Thr Ala 5cr 245 got gao tga act Ala Ala Cys Pro tag atc 5cr Ile 255 aaa an ctg Lys Lys Len gga ant att Gly As Ile 275 cgt gan tat Arg Ala 5cr ttt tag Ph. 5cr 265 gac ant avg vtg Asp Asn Thr Leu co gag ago tgt Pro Giu 5cr Cys ggcanag cat gtc Gly Lys Pro Val aea ttg ago Lys Len Arg 270 gta agg atg Vai Arg Met cac tgg otg His Trp Len tta gag phe Ala 290 ott gay gaa atg Len Asp Gin Leu agg one &gt Arg Gin 5cr ttt ea eac Phe Thr Amn 300 ggo ggo gga agg Gly Gly Gly Arg taa gao ggcaeta 5cr Asp Gly Ile ggo eta too gac ag aog Gly Ile 5cr Asp Thr Pro 315 320 ta aag cog ntg nag gee Set Lys Pro-Met Lys Gin 335 gee. ga gag ong Gin 'fly Ala Gin ott gao gtt goa cay Leu Ala Val. Ala His 330 1008 WO 01/85772 att ttg acg Ile Leu Thr gto ttc ctc Val Phe Leu 355 PCTGB1OZOO3 atg aao aag ogt Met Asn Lys Arg gac aat eta att Asp Asn Le Ile gog cgt toe Ala Mrg Sex 350 gtt too gaa Val 3cr Glu 1056 aaa oto. ggc ggc gac ggo aaa otg coo Lys Leu Sly Sly Asp (fly Lys Len Pro 360 1104 cag gca Gin Mla 370 gag tot gc gtc Ala Ser Ala Val ogt gas ott gc AMg Gin Len Mla tog ggc atc gat Sex Gly Ile Asp 1152 gog gat ttg gtt Ala Asp Leu Val gas aao ggt toa ggt ctg too aga aaa Gin Asn Sly Sex Sly Leu Sex Mrg Lys 395 1200 agg gta nog gcg Mrg Val Thr Ala- age cog ttt goa Sex Pro Phe Ala 420 atg atg gog as Met Met Ala Gin ttg gasaeg got Leu Gin Thr Ala tat tto Tyr Phe 415 1248 1296 osa gat ttc atc Gin Asp Phe Ile sog otg ccc atc gcc ggc sa Thr Leu Pro Ile Ala Sly Thr 430 gao ggg act Asp Sly Thr 435 tta cgo aao ogo Leu AMg Asn Mrg asa caa ago Lye Gint 8cr gga ggg Sly Sly 445 otg ttg ogo Len Leu Arg 1344 tta ass Len Lys 450 acc gge acg oto aso aat gt ecgc gc Thr Sly Thx Len Asn Aen Val Mrg Ala 455 gon ggt tat tgg Mla Sly Tyr *Txp 1392 ggo gao ass cog Gly Asp Lys Pro gog gtg gtc gte Ala Val Val. Val ate aao ago ggo Ile Asn Sex Gly 1440 gec gtt too otg Ala Val Sex Len ccc gac ttg gao Pro Asp Leu Asp tto gtt gcC Phe Vai Mla aao sac ato Asn As Ile 495 tgc asa gaa Cys Lys Sin 510 1488 1536 Ate too ggc Ile Sex Sly ogo cga gc Arg Mg Mla 515 gat ggc tgg otg Asp Gly Trp Len gog ass otg atg Ala Lys Len Met 1545 WO 01/85772 PCT/GB01/02003 <210> 86 <211> 515 <212> PRT <213> Neisseria meningitidis <400> 86 Val Arg Leu Asn His Phe Ile Met Ile Ala Ile Ile Ile Tyr Val Ile 1 5 10 Ser Pro Ala Asn Lys Pro Ala Arg Arg His Ser Val Pro Thr Tyr Pro 25 Ala Leu Pro Tyr Asn Cys Phe Phe Tyr Val Thr Asp Leu Pro Met Asn 40 Phe Pro Lys Thr Ala Ala Ser Leu Leu Leu Leu Leu Ala Ser Leu Ala 55 Ala His Ala Leu Asp Thr Gly Arg Ile Pro Gin Asn Glu Ile Ala Val 70 75 Tyr Val Gin Glu Leu Asp Ser Gly Lys Val Ile Ile Asp His Arg Ser 90 Asp Val Pro Val Asn Pro Ala Ser Thr Met Lys Leu Val Thr Ala Phe 100 105 110 Ala Ala Phe Lys Thr Phe Gly Ser Asn Tyr Arg Trp Ala Thr Glu Phe 115 120 125 Lys Ser Asn Gly Thr Val Asn Asp Gly Thr Leu Asp Gly Asn Leu Tyr 130 135 140 Trp Ala Gly Ser Gly Asp Pro Val Phe Asn Gin Glu Asn Leu Leu Ala 145 150 155 160 Val Gin Arg Gin Leu Arg Glu Gin Gly Ile Arg Asn Ile Thr Gly His 165 170 175 Leu Met Leu Asp His Ser Leu Trp Gly Glu Val Gly Ser Pro Asp Asp 180 185 190 Phe Glu Ala Asp Ser Gly Ser Pro Phe Met Thr Pro Pro Asn Pro Thr 195 200 205 Met Leu Ser Ala Gly Met Val Met Val Arg Ala Glu Arg Asn Ala Ala 210 215 220 WO 01/85772 Asp Ser Thr Asp 225 Gin Asn Asn Leu Lys Lys Leu Met 260 Gly Asn Ile Pro 275 Phe Ala Leu Asp 290 Leu Gly Gly Gly 305 Glu Gly Ala Gin Ile Leu Thr Asp 340 Val Phe Leu Lys 355 Gin Ala Ala Ser 370 Val Ala Asp Leu 385 Arg Val Thr Ala Ser Pro Phe Ala 420 Asp Gly Thr Leu 435 Leu Lys Thr Gly 450 Leu Gly Asp Lys 465 Pro Leu 235 Gln Ala 250 Asp Asn Lys Pro Ser Phe Ile Gly 315 His Ser 330 Asp Asn Lys Leu Leu Ala Ser Gly 395 Met Leu 410 Thr Leu Gin Ser Arg Ala Val Ile 475 Pro His Ala Cys Thr Leu Val Gly 285 Thr Asn 300 Ile Ser Lys Pro Leu Ile Pro Ala 365 Val Ser 380 Leu Ser Glu Thr Pro Ile Gly Gly 445 Leu Ala 460 Ile Asn PCT/GB01/02003 Ala 240 Ile SArg SMet Leu Pro 320 SGlu Ser Glu Asp i Glu 400 Phe Thr SArg Trp SArg 480 WO 01/85772 PCT/GB01/02003 Ala Val Ser Leu Leu Pro Asp Leu Asp Asn Phe Val Ala Asn Asn Ile 485 490 495 Ile Ser Gly Gly Asp Gly Trp Leu Asp Ala Lys Leu Met Cys Lys Glu 500 505 510 Arg Arg Ala 515 <210> 87 <211> 840 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 87 atg gat aaa gaa cga Met Asp Lys Glu Arg 1 ctg ctg cat ttg gca Leu Leu His Leu Ala ccc gtg ata gag tca Pro Val Ile Glu Ser ttt ggc gga ggg gac Phe Gly Gly Gly Asp ccc gaa ccg caa ccc Pro Glu Pro Gin Pro aag ccg gtt ttg aag Lys Pro Val Leu Lys cag cag cct aag gaa Gin Gin Pro Lys Glu att tta acc ccc Ile Leu Thr Pro atg gtg gca ttg Met Val Ala Leu ggc aat gtt att Gly Asn Val Ile 40 ggc gca ccc gaa Gly Ala Pro Glu 55 gtg ccc gag ccg Val Pro Glu Pro 70 ccg gtg gtt acg Pro Val Val Thr gag ccg aaa cct Glu Pro Lys Pro 171 gtc gtg ttt Val Val Phe tgg cag gcg Trp Gln Ala ttt gtc gat Phe Val Asp gca ggc gcg Ala Gly Ala aaa cct gtc Lys Pro Val aag gcg gat Lys Ala Asp gaa aag ccg Glu Lys Pro WO 01/85772 WO 0185772PCT/GBO1/02003 gas aaa cog Glu Lye Pro 115 saa ova gag cot aag cog gaa gog sag Lys Pro Glu Pro Lys Pro Giu Mla Lys 120 gtc cog aaa Val Pro Lys cog gcg gas aaa cog gto Pro Ala Glu Lys Pro Val 130 aag cog tct gas asa cot goc gas cat Lys Pro 5cr Gin Lys Pro Mla Glu 140 ggc aat got tot goc aaa gos gao ago ely PAsn Ala 5cr Mla Lys Mla Asp 3cr 150 oag ggo aat ggg gaa Gin Gly PAn Sly Gin 160 gat aag gga ac0 ggt ato aaa gga gao gga aog ggg ogo gga Asp Lys Gly Thr Gly Ile Lys Gly Asp Gly Thr Gly Arg Sly 165 170 ago ggt aaa ggt Set Sly Lys Sly 180 ggc ago ago ass Sly 3cr 3cr Lys 195 cog got tat Coo Pro Ala Tyr Pro 210 ago ggo ggt gto 5cr Sly Gly Val ggc gas cac ggg Sly Gin His Gly gaa gga goo Gin Sly Mla 190 att cog ogo Ile Pro Mrg ggc aat cot Sly an Pro ogo goo sac ggc Arg Mla an Sly sog ott. tot atg Thr Leu 5cr Met gag sat gat Gin PAn Ap cag ggt sog gtt Gin Sly Thr Val ttg ago gtt ttg gtt tot cog ggc ggt oat gtt gag too gtt Len 5cr Val Len Val 3cr Pro Sly Sly His Val Gin 5cr Val ato gtg a ago Ile Val Lys 3cr ggt ttt too ogg Sly Phe Ser Mxg gao ast gog gos Asp Asn Ala Ala ogc sag Mzg Lys 255 gog gog oa Mla Mla Gi1n gtC coo gto Val Pro Val. 275 ggg cat ttt OSS Sly His Phe Gln sat gcc tgg sog Asn Mla Trp Thr gag ttt ass GJlu Phe Lys 270 sag ttt gsa ttg aat Lys Phe Gin Len Asn <210> Se WO 01/85772 <211> 280 <212> PRT <213> Neisseria <400> 88 Met Asp Lys Glu 1 Leu Leu His Leu Pro Val lie Glu Phe Gly Gly Gly Pro Glu Pro Gin Lys Pro Val Leu Gin Gin Pro Lys 100 Glu Lys Pro Lys 115 Pro Ala Glu Lys 130 Pro Gly Asn Ala 145 Asp Lys Gly Thr Ser Gly Lys Gly 180 Gly Ser Ser Lys 195 Pro Ala Tyr Pro 210 PCT/GB01/02003 meningitidis Arg 5 Ala Ser Asp Pro Lys Glu Pro Pro Ser Gly 165 Ser Gly Thr Ile Leu Thr Met Val Ala Gly Asn Val 40 Gly Ala Pro 55 Val Pro Glu 70 Pro Val Val Glu Pro Lys Glu Pro Lys 120 Val Glu Lys 135 Ala Lys Ala 150 Ile Lys Gly Gly Gly Val Asn Pro Leu 200 Leu Set Met 215 Pro Ala Leu Leu Ile Glu Glu Gly Pro Pro Thr Lys Pro Glu 105 Pro Glu Pro Ser Asp Set Asp Gly 170 Lys Gly 185 Arg Ala Glu Asn Val Trp Phe Ala Lys Lys Glu Ala Glu Glu 155 Thr Glu Asn Asp Val Phe Gin Ala Val Asp Gly Ala Pro Val Ala Asp Lys Pro Lys Pro 125 Lys Pro 140 Gin Gly Gly Arg His Gly Gly Ser 205 Glu Gin 220 Val Leu Set Val Leu Val Set Pro Gly Gly His Val Glu Ser Val Lys 173 WO 01/85772 PCT/GB01/02003 225 230 235 240 Ile Val Lys Ser Ser Gly Phe Ser Arg Leu Asp Asn Ala Ala Arg Lys 245 250 255 Ala Ala Gin Ann Gly His Phe Gin Ala Asn Ala Trp Thr Giu Phe Lys 260 265 270 Val Pro Val Lys Phe Giu Leu Asn 275 280 <210> 89 <211> 1584 <212> DNA <213> Neisseria meningitiis <220> <221> CDS <222> (1)..f1584) <400> 89 atg gca Met Ala 1 atg gtg Met Val gca gtg Ala Val atg ctg Met Leu ccg att Pro Ile acc acg Thr Thr ccc aac cit tic cic ago ata ttc cce atc gtc ctg ctg ati Leu Phe Leu Ser Ile Phe Pro Ile Val Leu Leu Ile eac agt atg ccc too Asn Ser Met Pro Set tac gcc atc aaa ctt Tyr Ala Ile Lys Len goc aco gcc gct too Ala Tir Ala Ale 8cr ati ttc ggc gcg att Ile Phe Gly Ala Ile 70 etc gat gic atc cgc Ile Asp Val Ile Arg gcg caa ctg aig ati 174 tgg ctg Trp Leu ati acc lie Thr gog ggc Ala Gly cic ecg Leu Thr atg gaa Met Glu etc ago ile Ser gcc ttt WO 01/85772 WO 0185772PCT/GBOI/02003 Pro Asn Pro Val Ala 100 Gin Len Met Ile le Gly Trp Ala 105 Phe Ala Phe 110 atg att gas ggc ges tec ggc ttc ggc acg oct gc gcg att gc geg Met Ile Giu Gly Ala 5cr Gly Phe Gly Tin Pro Ala Ala Ile Ala Ala ccg att Pro Ile 130 ctg atg agc ttg Leu Met Ser Leu ttc aac ccg ttg aaa gtg gcg att ttc Phe Asn Pro Len Lys Val Ala Ile Phe 140 ttg gtg atg ase tcc gtc ccc gtc tct Len Val Met Asn 9cr Val Pro Val Ser 150 ggc gcg gtc ggt Gly Ala Val Gly cog ace tgg ttc ggt ttc gca ccg ctg sac otg agt gac gas. Pro Thr Tip Phe Gly Phe Ala Pro Leu Asn Leu 5cr Ala Gin gac atc Asp Ile 175 etc gee ate Leu Ala Ile gtc ate ccc Val Ile Pro 195 agg cag acc ggc Arg Gin Thr ely atg cac ttc ttc Met His Phe Phe gca ggt ttc Ala Gly Phe 190 tot gaa atc 5cr Glu Ile gtc ate ggc ttg Vai Ile Giy Len tte atc gts cot Phe Ile Val Pro cgc aaa sac ttg ggc ttc gtc gec att goc gtc Arg Lys Asn LeU Gly Pha Vai Ala Ile Ala Val tcc tgc aco att 5cr Cys Thr Ile tat gte gos ttg Tyr Val Mla Leu atg gtc sac ga Met Val Asn Gin ttc cog tcg ctc Phe Pro 8cr Leu goc ggc gog ato Ala ely Mla Ile ctg atg gtg tec Len Met Val Ser ttc goc gcc sac Phe Ala Mla Asn caa ggc Gin Gly 255 tgg ggo ttg Tip ely Leu aaa gao cac gcc Lys Asp His Ala gac ccg sat gc Asp Pro Asn Mla gaa aaa gtg Gin Lys Vai 270 atg ctg ste Met Len Ile cog ttc goc caa Pro Phe Ala Gin 275 gtc gcc aaa Val Ala Lys ctc gcc cct ttg Len Ala Pro Leu ggc atg otg gtg gtt aog cgc ste aaa cag etc ggo ato aaa ggc att WO 01/85772 WO 0185772PCTIGBO1IO2003 Gly met 290 Leu Val Val Thr Ile Lys Gin Lea. Ile Lys Gly Ile acc agc ann gan gan tgg ttc agc ttc can etg cog ttt gat Thr 5cr Lys Giu Giu Ttp Phe Ser Phe Gin Lou Pro Phe Asp tcc an atc ace Lys Ile Thr ttc gga can gat Phe Giy Gin Asp 340 agc gac tcc ctg Set Asp 3cr Lcu ntt act ttc ggc Ile Thr Phe Gly ant att. Asn Ile 335 1008 gtc agc gcg tot Val Set Ala 3cr can ncg ctg Gin Thr Leu tac gtc ccg get Tyr Vnal Pro Ala 350 ctg ctg tat ann Leu Lou Tyr Lye 365 1056 tgg att ccg ttt gtg ctg ncc Trp Ile Pro Phc Val Lou Thr 355 tgg att tgc ate Trp Ile Cys Ile 1104 1152 ace ann Thr Lys 370 ttc an gat 9CC Phc Lye Asp Mla acg att tat tcc Thr Ile Tyr Set gta act Vni Thr 380 ttc ant cnn Phe Asn Gin ace ann ann ccg ctg ctt gcc ctg atg ggc Thr Lys'LyS Pro Leu Leu Ala leu Met Giy 385 390 ctg ntg ctg gtc ggc ggc gac ant- tcg ntg Lou Met Leu Vai Gly Giy Asp Ann Set Met 405 410 etg att atg gtt. Leu Ile Met Vai 1200 1248 gtg aan ate ate Vai Lys Ile Ile ggt nag Giy Lye 415 gan ttt gcc gca atg gcg ggc ga Giu Phe Mla Ala Met Ala Gly Giu tgg gtt tat ttc Trp Val Tyr Phe tcg ccg tat Set Pro Tyr 430 gtg toc nec Val 3cr Asn 1296 1344 ctg ggc gcg Leu Giy Ala 435 ato ggt gcg tte ttc tee ggt tec nac 110 Gay Mla Phe Phe Set Gly 3cr Asn 440 ctg ae Leu Thr 450 ttc ggc ceg att Phe Gly Pro Ile eng can ntc gee ttg gat ae ggc ctg Gin Gin Ile Ala Lcu Asp Thr Gly Lew 460 1392 gtt acc ctg att. Val Thr Leu Ile gag ttg cag tce gtc Aln Lea Gin Set Val 475 ggc ggc gcg atg ggc Gly Giy Ala Met Gly 480 1440 ant atg gtg tgc ctc sac nac ate ate goc gta tgt ae gtn ttg gat 18 1488 WO 01/85772 PCT/GB01/02003 Agn Met Val Cys Leu Asn Asn Ile Ile Ala Val Cys Thr Val Leu Asp 485 490 495 gtg aaa aat tcc gaa ggt gcg att atc aag aaa acc gtt atc ccg atg 1536 Val Lys Asn Ser Glu Gly Ala Ile Ile Lys Lys Thr Val Ile Pro Met 500 505 510 gcg att tac ggc gtg att gcc gtc gtc gcg gca atg att ttc ttc ctc 1584 Ala Ile Tyr Gly Val Ile Ala Val Val Ala Ala Met Ile Phe Phe Leu 515 520 525 <210> <211> 528 <212> PRT <213>.Neisseria meningitidis <400> Ile Val Leu Tyr Val Ala Phe Tyr Phe Gly Leu Val Met Phe Asn Lys Trp Leu Ile Gly Trp Thr Pro Ala Pro Leu Lys 140 Ser Phe Gly 155 Leu Thr Gly Thr Glu Set Phe Ala Phe Thr 160 WO 01/85772 PCTIGBO1IO2003 Pro Thr Trp Phe Gly Phe Ala Pro Leu lAin Leu Ser-Al1a 0hz Asp Ile 165 170 175 Gly Val 185 Gly Phe 200 Ala Ile Val lAsn Val Set Ala Lys 265 Ala Leu 280 Ile Lys Phe Leu Tyr 345 Val Trp 360 Thr Ie Leu Met lAsn Ser WO 01/85772 Glu Phe Ala Leu Gly Ala 435 Leu Tbr Phe 450 Ser Val Thr 465 Asn Met Val Val Lys Asn Ala Ile Tyr 515 Ala Gly Ala Phe Ile Gin 455 Leu Ala 470 Asn Asn Gly Ala Ile Ala f I <210> 91 <211> 1686 <212> DNA <213> Neissexia moningitidis <220> <221> CDS <222> (1)..(1686) <400> 91 atg tta caa tcc gaa aat tcg Met Lou Gin Ser Glu An er 1 gct tta caa atc atg att tct Ala Leu Pro Ile Met Ile Ser 9 gcg aag ccg ctg ttt gaa aac c Ala Lys Pro Leu Phe Glu Asn 1 cgc.tac ggt ttg atc ggc gcg E Arg Tyr Gly Leu Ile Gly Ala z Glu Phe 440 GIn Leu Ile Iie al 520 maa 1 ys IcC 'hr ;ta Tal ac ~sn 4 179 Val Tyr Ser Asn Ala LCU 460 Val Gly 475 Val Cys Lys Thr Ala Met Phe Ser 430 Thr VaX 445 Asp Thr Gly Ala Thr Val Val Ile 510 Ile Phe 525 PCT/GBOI/02003 Tyr Asn Leu Gly 480 Asp Met Leu WO 01/85772 aaa ate etc ggo Lye Ile Leu Gly gaa aac ggc gtg Glu Asn Gly Val gaa gat atg oge Giu Asp Met Arg 100 tgg geg geg atg Trp Ala Ala Met 115 acc gaa gac gee Tb: Glu Asp Asp 130 gee tae gac gge Glu Tyr Asp Gly 145 ggc gtg gge att ely Val Gly Ile gee ccg ggC tte Ala Pro Gly Phe 180 aag ceg gat gta Lys Pro Asp Val 195 aat ace atc cgt Asn Tb Ile Avg 210 atg att ate ate Met Ile Ile le 225 cat atg gca gat His Met Ala Asp cag aca gee ggc gaa Gin Thy Ala Sly Giu 75 ttg ego caa gac Cag Lou Avg Gin Asp Gin gta atg atg ggt cac Val Met Met Gly His 105 geg att tee gee eat Ala Ile Tyr Ala ken 125 gee gaa etg gaa gee Ala GTh Leu Giu Ala 140 geg cgt gee gee gag Ala Avg Ala Ala Glu .155 cac eat gee ace atg His ken Ala Thy Met 170 ttg ctg geg caa gee Leu Leu Ala Gin Ala 185 gaa ceg ace aat aec Glu Pro Thy Asn ken 205 gtg ttg aae cag tao Val Leu ken Gin Tyr 220 eec tte ttg aae gaa His Phe Leu ken Giu 235 ace ate ace ate tat Th Ile Thr Ile Tyr 250 PCT/GBOI/02003 fatt 240 kIle tee 288 LTyr Latg 336 LMet Lgee 384 LAla gee 432 Ala rage 480 160 gtt 528 tVal tee 576 S er att 624 SIle aeg 672 Thr aeg 720 Thr 240 aec 768 Asn WO 01/85772 WO 0185772PCT/GBO1/02003 tac gac gae tat Tyr Asp Asp Tyr 260 aaa gac aat gee Lys Asp Ann Al-a 275 atg etc gee Met Leu Ala aag gcg aag Lys Ala Lys tee gee 8cr Ala 265 gaa aaa Gin Lys 280 eaa teg ege gaa Gin Ser Arg Gin egc gee etg Arg Ala LeU 270 eaa gaa ttt Gin Gin Phe ttg eag gas Leu Gin Gin gtg geg egg ttc agt gee aac ana tee aaa gee Val Ala Arg Phe 8cr Ala Ann Lys 8cr Lys Ala cag gca ae agc Gin Ala Thr Ser etg aaa eag ge. Leu Lys Gin Ala aaa ate aaa tcg gag atg gte gag gto Lys Ile Lys Ser Gin Met Va1 Gin Val 315 ccc tet ace egt Pro Set Thr Arg aac ccg tat ate Asn Pro Tyr Ile ttt gaa gee gat gaa aaa Phe Gin Ala Asp Gin Lys 335 1008 gee .aag etg Ala Lys Len ttt gaa ace Phe Gin flit 355 cgt eag get gtg Arg Gin Ala Val gaa gtt gaa aaa etg gcg aaa ege Gin Val Gin Lys Len Ala Lys Arg 345 350 1056 eag ttg ttt aan Gin Len Phe Lys etg aae ttt ate Len Ann Ps Ile gaa geg gga Gin Ala Gly 1104 eaa ego Gin Arg 370 etc gce ate ate Len Ala Ile Ile ceg Sae gge gcg gge aaa tee ace etg Pro Asn Gly Ala Gly Lys 8cr Thr Len 380 1152 aaa etc ttg gee LYS Len Len Ala geg tac aac ce Mla Tyr Asn Pro tat tea gae ggt Tyr Set Asp Gly 1200 1248 etg ceg gae gaa Len Pro Asp Gin age ate asa tgg 5cr Ile Lys Trp gaa aaa gee age Gin Lys Ala 5cr gte gge Val Gly 415 tac tat ceg Tyr Tyr Pro age gaa tgg 8cr Gin Trp 435 gat cac gaa aae gao Asp His Gin Aen Asp 425 tto gat gte gat Phe Asp Val Asp atg gat ttg Met Asp Leu 430 1296 atg ege eaa tgg ggg cag gee gge gat gac gaa eaa gte Met Arg Gin Trp Gly Gin Asp Gly Asp Asp Gin Gin Val 1344 WO 01/85772 PCT/GB01/02003 gat gtc gtg 1392 Asp Val Val aetc cgc ggc act ttg ggg cgt Ile Arg Gly Thr Leu Gly Arg 450 455 aaa aaa gtg aag gtt ctc tcc Lys Lys Val Lys Val Leu Ser 465 470 ggc aaa ctg ttg ctg ttg aaa Gly Lys Leu Leu Leu Leu Lys 485 acc aac cac atg gac atg gaa Thr Asn His Met Asp Met Glu 500 gaa aaa tac aac ggc acg ctg Glu Lys Tyr Asn Gly Thr Leu 515 gtt tct tcc ctg got acc caa Val Ser Set Leu Ala Thr Gn 530 535 tat .gaa cac tac ttg ggc gat Tyr Glu His Tyr Leu Gly Asp 545 550 gta gca Val Ala <210> 92 <211> 562 <212> PRT <213> Neisseria meningitidis <400> 92 Met Leu Gin Ser Glu Asn Ser 1 5 Ala Leu Pro Ile Met Ile Ser Ala Lys Pro Leu Phe Glu Asn ttg ctc ttc Leu Leu Phe agt aac Ser Asn 460 ggc cgt Gly Arg gtc atg Val Met ttg aac Leu Asn cac gac His Asp 525 gac ggc Asp Gly 540 ctc gag Leu Glu 1440 1488 1536 1584 1632 1680 1686 Lys Asn Leu Ile Sex Cys Ser Ile Lys 10 Thr Asn Gly Ile Thr Met Gin Phe Gly 25 Val Set Val Lys Phe Gly Glu Gly Asn 40 182 WO 01/85772 Arg Tyr Gly Lys Ile Leu Glu Asn Gly Glu Asp Met Trp Ala Ala 115 Thr Glu Asp 130 Glu Tyr Asp 145 Gly Val Gly Ala Pro Gly Lys Pro Asp 195 Asn Thr Ile 210 Met Ile Ile 225 His Met Ala Tyr Asp Asp Lys Asp Asn 275 Ile Gly Ala Asn 55 Gly Asp Leu Glu 70 Arg Leu Gly Lys Val Leu Asp Val Thr Glu Arg Asp 120 Tyr Met Lys Ala 135 Tyr Thr Ala Glu 150 Ser Glu Asp Leu 165 Lys Leu Arg Val Leu Leu Leu Asp 200 Trp Leu Glu Gly 215 Ser His Asp Arg 230 Leu Asp Tyr Asn 245 Met Leu Ala Ser Lys Ala Lys Glu 280 Lys Ser Gly Glu Asp Gin Gly His Ala Asn 125 Glu Ala 140 Ala Glu Thr Met Gin Ala Asn Asn 205 Gin Tyr 220 Asn Glu Ile Tyr Arg Glu Glu Leu 285 PCT/GB01/02003 Met Ile Tyr Met Ala Ala Ser 160 Val Ser Ile Thr Thr 240 Asn Leu Phe val Ala 290 Arg Phe Ser Ala Lys Ser Lys Ala Gin Ala Thr Ser WO 01/85772 Arg Leu Lys 305 Pro Ser Thr Ala Lys Leu Phe Glu Thr 355 Gin Arg Leu 370 Leu Lys Leu 385 Leu Pro Asp Tyr Tyr Pro Ser Glu Trp 435 Ile Arg Gly 450 Lys Lys Val 465 Gly Lys Leu Thr Asn His Glu Lys Tyr 515 Val Ser Ser 530 Tyr GClu His 545 Gin Ala Arg Gin 325 His Arg 340 Gin Leu Ala Ile Leu Ala Glu Gly 405 Gin Asp 420 Met Arg Thr Leu Lys Val Leu Leu 485 Met Asp 500 Asn Gly Leu Ala Tyr Leu Asp Lys 310 Asn Pro Gin Ala Phe Lys Ile Gly 375 Gly Ala 390 Ser Ile His Glu Gin Trp Gly Arg 455 Leu Ser 470 Leu Lys Met Glu Thr Leu Thr Gin 535 Gly Asp 550 Ser Glu Met 315 Arg Phe Glu 330 Val Glu Lys Asn Phe Ile Gly Ala Gly 380 Pro Glu Tyr 395 Ala Glu Lys 410 Phe Asp Val Asp Gly Asp Phe Gly Ser 460 Glu Lys Gly 475 Val Leu Val 490 Glu Ser Leu Val Ser His Glu Leu Asp 540 Ser Tyr Leu 555 PCT/GB01/02003 SLys 320 I Lys SArg L Gly SLeu Leu 400 Gly Leu i Val SVal STyr 480 Pro Leu Phe Gly Gly 560 Arg Met Asn Asp 525 Gly Glu WO 01185772 PCTIGBO1IO2003 Val Ala <210> 93 <211> 1126 <212> DNA <213> Neisseria ineningitidis <220> <221> CDS <222> (1125) <400> 93 atg eec aco tog cee cgc aec ago aftc gtc ago ago tgg aftc eec too 48 Met Asn Thr Set Gin Arg PAn Arg Leu Val 3cr Arg Trp Leu PAn 1 5 10 tao gaa ago tad cgc tac cgc ogo oto ae cac goc gtc ogg otc ggc 96 Tyr Glu Arg Tyr Arg Tyr Arg Arg Lou Ile His Ala Val Arg Lou Gly 25 ggg gee gtc otg ttc geo aco goc too gco cgg atg etc ceo cto ose 144 Gly Ale Val Lou Phe Ala Thr Ala Sex Ala Axg Lou Leu His Lou Gin 40 ceo ggc gag tgg ate ggg atg aca gta ttc gtc gto oto ggc atg oto 192 His Gly Glu Ttp Ile Gly Met Thr Val Phe Veal Val Lou Gly Met Leu 55 ceg frtt caa ggg gcg att tao tec aeg gag gtg gee ogt etg oto ggo 240 Gin Phe Gin Gly Ala Ile Tyr Set Lys Ala Val Giu Arg Met Lou Gly 70 75 cg gtc ae g~g atg ggo gcg ggt ttg ggo gtt tta tgg otg eec cag 288 Thr Vel Ile Gly Leu Gly Ala Gly Lou Gly Vai Leu Trp Lou Asn Gin 90 oat tat ttc ceo ggo ac oto afo tc tao ofro aco gtc ggc ag gaa 336 His Tyr Phe His Gly PAn Leu Lou Phe Tyr Lou Thr Val Gly Thr Ale 100 105 110 agc goacotg gcc ggc Egg gag gog gfrc ggc cee aco ggc tea gtc oat 384 Ser Ale Lou Ale Gly Trp Ala Ala Vai Sly Lys an sly Tyr Vai Pro 115 120 125 atg atg gag ggg ctg ag efrg Ego atg otc ae gga gac aac ggc age 432 WO 01/85772 PCT/GBOI/02003 Met Len Ala Gly Lau Tin Met Cys Met Len Ile Gly Asp Aan Sly Sex 130 135 140 gaa tgg tte gao age gge etg atg ce geg atg aae gte etc ate gge 480 Gin Trp Pile Asp Set Gly Leu Met Arg Ala Met Asn Val Len Ile Gly 145 150 155 160 geg gee atc gee ate gcc gcc gcc aaa ctg ctg cog otg aaa tec aca 528 Ala Ala Ile Ala Ile Ala Ala Ala Lys Leu Leu Pro Len Lys Ser Thr 165 170 175 etg atg tgg egt ttc atg ott gee gao aae etg aee gae tge age aaa 576 Lan Met Trp Arg Phe Met Len Ala Asp Asn Len Tilt Asp Cys Ser Lys 180 185 190 atg att gee gas ate age aae gge agg ege atg ace ege gas ege etc 624 Met Ile Ala Gin Ile Sex Asn Gly Arg Arg Met Tilt Arg Gin Arg Leu 195 200 205 gas gag ase atg geg aaa atg ege eaa ate sac ges ege atg gte usa 672 GInu Gin Asn Met Ala Lys Met Arg Gin Ile Asn Ala Arg Met Val Lys 210 215 220 age ego age eac etc gee gee aea teg gge gas age ege ate age ccc 720 Ser Axg Sex His Leu Ala Ala Thr Sex Gly Gin Sex Arg Ile Ser Pro 225 230 235 240 gee atg atg gaa gee atg eag eac gee cae egt aaa att gte sac ace 768 Ala Met Met Gin Ala Met Gin His Ala His Arg Lys Ile Val Asn Thr 245 250 255 ace gag ctg etc ctg ace ace gee gee aug etg cas tet coo sea etc 816 Thr Gin Len Len Len Tilt Thr Ala Ala Lys Len Gin Sex Pro Lys Leu 260 265 270 aae ggc age gaa ate egg ctg ett gao ege cue tto aca ctg etc ena 864 Asn Gly Sex Gin Ile Arg Len Lou Asp Arg His Phe Tilt Len Leu Gin 275 280 285 ace gac ctg caa cas ace gte gee ott ate ase gge aga eac gee ege 9i2 Tilt Asp Len Gin Gin Thr Val Ala Len Ile Asn (fly Arg His Ala A-rg 290 295 300 egc ate ego ate gac ace gee ate use coo gsa etg gaa gee etc gee 960 Arg Ile Arg Ile Asp Tin Ala Ile Asn Pro Gin Len Gin Ala Len Ala 305 310 315 320 gas cue etc cac tac esa tgg eag gge tto etc tgg etc age see aat 1008 186 WO 01/85772 Glu His Leu His Tyr 325 atg cgt cag gaa att Met Arg Gin Glu Ile 340 cgc aaa tgg ctg gat Arg Lys Trp Leu Asp 355 ctt gaa aca cgg gaa Leu Glu Thr Arg Glu 370 <210> 94 <211> 375 <212> PRT <213> Neisseria menJ <400> 94 Met Asn Thr Set Gin 1 5 Tyr Glu Arg Tyr Arg Gly Ala Val Leu Phe His Gly Glu Trp Ile Gin Phe Gin Gly Ala Thr Val Ile Gly Leu His Tyr Phe His Gly 100 Set Ala Leu Ala Gly 115 Met Leu Ala Gly Leu PCT/GB01/02003 Trp Gin Gly Phe Leu Trp Leu Ser Thr Asn 330 335 gcc ctc gtc ate ctg ctg caa cgc acc cgc 1056 Ala Leu Val Ile Leu Leu Gin Arg Thr Arg 345 350 cac gaa cgc caa cac ctg cgc caa age ctg 1104 His Glu Arg Gin His Leu Arg Gin Ser Leu 360 365 agt t 1126 Ser 375 ingitidis Arg Asn Arg Tyr Arg Arg Ala Thr Ala 40 Gly Met Thr 55 Ile Tyr Ser 70 Gly Ala Gly Asn Leu Leu Trp Ala Ala 120 Thr Met Cys Leu Val Leu Ile 25 Ser Ala Val Phe Lys Ala Leu Gly Phe Tyr 105 Val Gly Met Leu Ser Arg Trp Leu His Ala Val Arg Arg Leu Leu His Val Val Leu Gly Val Glu Arg Met Val Leu Trp Leu Leu Thr Val Gly 110 Lys Asn Gly Tyr 125 Ile Gly Asp Asn WO 01/85772 PCT/GB01/02003 Glu Trp Phe Asp Ser Gly Leu Met Arg Ala Met Asn Val Leu Ile Gly 145 150 155 160 Ala Ala Ile Ala Ile Ala Ala Ala Lys Leu Leu Pro Leu Lys Ser Thr 165 170 175 Leu Met Trp Arg Phe Met Leu Ala Asp Asn Leu Thr Asp Cys Ser Lys 180 185 190 Met Ile Ala Glu Ile Ser Asn Gly Arg Arg Met Thr Arg Glu Arg Leu 195 200 205 Glu Glu Asn Met Ala Lys Met Arg Gin Ile Asn Ala Arg Met Val Lys 210 215 220 Ser Arg Ser His Leu Ala Ala Thr Ser Gly Glu Ser Arg Ile Ser Pro 225 230 235 240 Ala Met Met Glu Ala Met Gin His Ala His Arg Lys Ile Val Asn Thr 245 250 255 Thr Glu Leu Leu Leu Thr Thr Ala Ala Lys Leu Gin Sex Pro Lys Leu 260 265 270 Asn Gly Ser Glu Ile Arg Leu Leu Asp Arg His Phe Thr Leu Leu Gin 275 280 285 Thr Asp Leu Gin Gln Thr Val Ala Leu Ile Asn Gly Arg His Ala Arg 290 295 300 Arg Ile Arg Ile Asp Thr Ala Ile Asn Pro Glu Leu Glu Ala Leu Ala 305 310 315 320 Glu His Leu His Tyr Gin Trp Gin Gly Phe Leu Trp Leu Ser Thr Asn 325 330 335 Met Arg Gin Glu Ile Ser Ala Leu Val Ile Leu Leu Gin Arg Thr Arg 340 345 350 Arg Lys Trp Leu Asp Ala His Glu Arg Gin His Leu Arg Gin Ser Leu 355 360 365 Leu Glu Thr Arg Glu His Ser 370 375 WO 01/85772 <210> <211> 1725. <212> DNA <213> Neisseria meningitidis <220> <221> ODS <222> (1725) <400> PCTIGBOI/02003 atg Met 1 ggt Gly tac Tyr cac His ggt Gly gca Ala gtc Val caa Gin ttc Phe ttc ggo gcg ona Gly Ala Gin gtt ttC ggt Val Phe Gly ttc caa etc Phe Gin Leu gca gta cac Ala Val. His gtg gca ttg Val Ala Leu att gct act Ile Ala Thr cay gta, ggc Gin Val. Sly acg gtg ggt Thr Val Sly gao ato ant Asp Ile Asn 135 gca agc ggc agt etc 5cr Leu gog gtt Ala Val one att His Ile tat gcg Tyr Ala ccg ggc Pro Giy Phe Gin Ile Ala. Ala 5cr Gly Arg Pro Gly Pro Val Val Val Asp Vai WO 01/85772 WO 0185772PCT/GBOI/02003 cog aaa Pro Lys 150 gat gtt acg cag gcg atg Asp Val Thr Gin Ala Met 165 gog aaa ttc ago Ala Lys Phe Set 170 tat ct cag Tyr Pro Gin 175 528 gao att ttt ate ogt tog tat oaa cog gtt gtt Oaa Asp Ile Phe Ile Arg Ser Tyr Gin Pro Val Val Gin 180 185 ggg cat ate ggt Gly His Ile Gly 190 cag att aaa Gin Ile Lys 195 aag goc gtg cag atg ttg goa tot gee aaa ogt cog gto Lys Ala Val Gin Met Leu Ala Ser Ala Lys Arg Pro Val gto tat ttt ggc ggc ggo gtg gtg ttg ggt Val Tyr Phe Gly Gly Gly Val Val Leu Gly 210 215 aat got Aen Ala 220 tgc aog Cys Thr 235 tot gaa gag ctg Set Giu Gin Leu aco oga ttt gtco ga Thr Arg Phe Val Arg 225 aog ggt gct cg Thr (fly Ala Pro ggt acg otg Gly Thr Leu ggo ttg ggo got Gly Leu (fly Ala cot too ggo gao Pro Bar Gly Asp oaa tto oto ggt Gin Phe Len Giy atg oto Met Leu 255 ggt atg cac Gly Met His gto gtt ott Vai Val Len 275 act tao gag goa Thr Tyr Gin Ala ott gOC atg Leu Ala Met cag aat gog gat Gin Asn Ala Asp 270 gtg gtt too gta Val Val 5cr Val 285 goo gta ggt gog ogt ttt gao gac cgt Ala Val Gly Ala Arg Phe Asp Asp Arg 280 Cog too Pro Set 290 aaa ttt tto gag Lys Phe Phe Gin geg aag aag gtt ato oat ato gat gto Ala Lye Lys Val Ile His Ile Asp Vai 300 cot too ago ato Pro Ser Se Ile aaa ogo gtg aag Lye Arg Vai Lye gao att cog att Asp Ile Pro Ile ggc gao gtg aaa aao Giy Asp Vai Lye Asn 325 oaa gag too gtg cog Gin Gin 5cr Val Pro gtt ttg too gag Val Leu 5cr Gin gtt gog otg Vai Ala Leu tgg ona aaa Trp Gin Lys 335 1008 tot gas gat got ttg Set Gin Asp Ala Len ggo aaa tgg tgg ass ace Giy Lye Trp Trp Lye Thr 1056 WO 01/95772 WO 0185772PCT/GBO1/02003 ata Ile gaa Glu. ggc Sly 385 geg Ala ggc Gly aa Lys tcg 3cr att Ile 465 cgc Arg ttc Phe ate Ile gaa ggc* age Gly 8cr att ac Ilec Thr atg ttt Met Phe 400 aac tee Asn 3cr 415 ggt gCa sly Ala gac ggc Asp Sly tat egg Tyr Arg atg gta met Val 480 ace tat Thr Tyr 495 ggc cat Sly Hiis ttg ttg Lou Lou etg ace 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 Giu Ala Lcu Asn Gin Lys Asp Arg Lou Val Phe le Asp Phe Lcu Thr WO 01/85772 530 gac cag aaa cag Asp Gin Lys Gin 545 gac gaa atg gta Asp Glu Met Val <210> 96 <211> 575 <212> PRT <213> Neisseria PCT/GB01/02003 535 540 aat gtg atg ccc atg gtc ggc aac ggc aaa ggt ttg Asn Val Met Pro Met Val Gly Asn Gly Lys Gly Leu 550 555 560 ctt ccg cog cat atg cgt gcg gac gga aag gcg Leu Pro Pro His Met Arg Ala Asp Gly Lys Ala 565 570 575 meningitidis 1680 1725 <400> 96 Met Gin I 1 Gly Val C Tyr Asp His Glu C Gly Lys N Ala Leu 1 Val Ile S Gin Glu V 1 Phe Leu V 130 Phe Gin I 145 Pro Lvs P Gin Ile Gly Tyr Leu Asn His Ala 55 Leu Val Thr Ala Gly Asn Gly Ile 120 Asn Glu 135 Gly Arg Ala Met Ser Leu Ala Val His Ile Tyr Ala Pro Gly Ser Ile Gly Thr Cys Val 125 Thr Ile 140 Val Val Ser Tyr WO 01/85772 PCT/GB01/02003 Asp Ile Gln Ile Val Tyr 210 Thr Arg 225 Gly Leu Gly Met Val Val Pro Ser 290 Asp Pro 305 Gly Asp Gin Glu Ile Glu Glu Ile 370 Gly Asn 385 Ala Ala Gly Gly 165 Ile Arg 180 Lys Ala Gly Gly Val Arg Ala Tyr 245 Gly Thr 260 Ala Val Phe Phe Ser Ile Lys Asn 325 Val Pro 340 Trp Arg Lys Pro Ala Ile Tyr Tyr 405 Gly Thr Val Gln Ser Ala Asn Ala 220 Cys Thr 235 Gin Phe Ala Met Asp Arg Val Ile 300 Ala Asp 315 Val Ala Gly Lys Trp Phe Lys Leu 380 Gly Gln 395 Arg Gin Pro Tyr Gly His 190 Lys Arg 205 Ser Glu Gly Thr Leu Gly Gin Asn 270 Val Val 285 His Ile Ile Pro Leu Trp Trp Trp 350 Asp Asn 365 Ala Glu His Gin Trp Leu Ala Ile WO 01/85772 PCT/GB01/02003 420 425 430 Lys Leu Ala Ala Pro Asp Gin Asp Val Phe Cys Ile Thr Gly Asp Gly 435 440 445 Ser Ile Gin Met Asn Ile Gin Glu Leu Ser Thr Cys Phe Gin Tyr Arg 450 455 460 Ile Pro Val Asn Val Ile Thr Leu Asn Asn Gly Tyr Leu Gly Met Val 465 470 475 480 Arg Gln Trp Gin Glu Ile Tyr Tyr Gly Gly Arg Glu Ser Glu Thr Tyr 485 490 495 Phe Asp Ser Leu Pro Asp Phe Val Lys Leu Ala Glu Ala Tyr Gly His 500 505 510 Ile Gly Ile Arg Val Asp Lys Lys Ser Asp Val Glu Gly Ala Leu Leu 515 520 525 Glu Ala Leu Asn Gln Lys Asp Arg Leu Val Phe Ile Asp Phe Leu Thr 530 535 540 Asp Gin Lys Gin Asn Val Met Pro Met Val Gly Asn Gly Lys Gly Leu 545 550 555 560 Asp Glu Met Val Leu Pro Pro His Met Arg Ala Asp Gly Lys Ala 565 570 575 <210> 97 <211> 570 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 97 atg gac aat cac gcc gaa gca cac tgg caa aac ggc tgg ctt caa age 48 Met Asp Asn His Ala Glu Ala His Trp Gin Asn Gly Trp Leu Gin Ser 1 5 10 ata cgc cat ace ccg tcg ccc aat ttc agc ccg agg gaa acg ggg gaa 96 Ile Arg His Thr Pro Ser Pro Asn Phe Ser Pro Arg Glu Thr Gly Glu 25 194 WO 01/85772 WO 0185772PCT/GBO1/02003 aog gtt toc ctg ato gtg ttg cac aac att tos otg og og ttc gas Thr Val ser Leu Ie Val Lou His Asn le 5cr Lou Pro Pro Phe Glu tao ggo aog gat got gtg gaa aag otg ttt Tyr (fly Thr Asp Ala Val. flu Lys Lou Phe 55 sac gga cat cog tto ttc agc Ctg ata cac Asn Gly His Pro Pho Phe 5cr Lou Ile His 70 cat tto tts ato ass cgo gao ggc aaa acg His Phe Lou Ile Lys Arg Asp Gly Lys Thr 90 gco aao ogg ctc gao coo Ala Asn Arg Lou Asp Pro act ttg ogo gta tco agc Thr Leu Arg Val 5cr Ser 75 gtg cag ttc qta Val. Gin Phe Val tos tgo 8cr Cys ggc gat atg gog Gly Asp Met Ala 100 aaa tgo sac gos Lys Cys Asn Ala 115 tao oso gog ggo Tyr His Ala Gly too tog ttt ogo 8cr 5cr Phe Arg ggs cgg gas Gly Arg Glu 110 tgc gat tto Cys Asp PMe ttt toc ato Plie Scr Ile sto gas ttg ga Ile Giu Lou Glu gaa ccc Giu Pro 130 ttt acc gaa gcg hec Thr GlU Ala tao cgt tog ote Tyr Arg 8cr Lou aca ttg ttg ga Thr Lou Lcu Slu cto tgo ogo ogo tao coo gtt aoc gos gta acc gga cat cag Leu Cys Arg Arg Tyr Pro Val Thr Ala Val Thr Gly His Gin ato gcg coo ggo Ile Ala Pro (fly aaa soc gao 000 Lys Thr Asp Pro oso ttt tto gao His Pho PMe Asp tgg ogg Trp Arg 175 cgg sta ogg Arg Ile Arg aaa ggg ttt ccc gta Lys Gly Phe Pro Val 185 gao ags sat goo Asp Arg Asm Ala <210> 98 <211> <212> PRT <213> Noisseris meningitidis <400> 98 WO 01/85772 Met Asp Asn 1 PCT/GB01/02003 His Ala Glu Ala His Trp Gin Asn Gly Trp Leu Gin Ser Arg Ile Arg Glu Lys Gly Phe Pro Val Asp Arg Asn Ala Val 180 185 190 <210> 99 <211> 1515 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1515) <400> 99 WO 01/85772 atg ttg tat Met Leu Tyr 1 gtt tct gcc Val Sex Ala tac ggt ttt Tyr Gly Phe ggg gog gat Gly Ala Asp gon Ala gat Asp ttgj Leu a ac Asn aag Lys ctg Leu gaa Glu gog Ala 145 ttt Phe gtg Val ttg ttg Leu Leu tgg aa Trp Lys ttg gcg Leu Ala ggC aat Gly An ctg ccg Lieu Pro gcg aa Ala LyS 115 ctg cac Lieu His 130 gbg gcg Ala Ala gcc gag Ala Glu ggg cgt Gly Arg gtg cag cgg Val Gin Arg gad eat gat Asp Ann Asp 55 ccg aaa atg Pro Lys Met 70 geg gat ttg Ala Asp Lieu tat gac gcg Tyr Asp Ala gcg gga'agg Ala Gly Arg gaa aat gcg Gin Ann Ala 135 ttt gac gat Phe Asp Asp 150 gaa sea ttg Gin Lys Leu 165 cgg aaa aa Arg Lys Lys ttg gtt gtt tgg tgt Len Val Val Trp Cys gcg ccc gcg att ttg Ala Pro Ala le Leu gtg tog gat aag tgg Val 5cr Asp Lys Trp cog ogo gtg gtt gao Pro Arg Val Val Asp gaa cat agt ttg ogc Gin His Sex Len Arg 75 got tog ttg gog gat Ala Sex Leu Ala Asp ttg tao ggo agg gcg Len Tyr Gly Arg Ala 105 gog gag gog gtg gcg Ala Glu Ala Val Ala 125 gao gag ogg att ttg Asp Glu Arg Ile Lieu 140 cgg ctg aag tog gca Arg Len Lys 5cr Ala 155 ttg Ccg gog cg gtt Len Pro Ala Pro Val 170 PCT/GBOI/02003 ggt 48 LGly aag 96 Lys Ltog 144 ttt 192 Phe oto 240 *Len goc 288 Ala ttg 336 LLen *cgg 384 Arg ttg 432 Leu cat 480 1His 160 at 528 *Asn gag ggg ctg cg ggc tgg cgt ttt Gin Gly Len Thr Gly Trp Arg Phe WO 01/85772 tog ggc ggc Set Gly dly 195 PCT/GBOI/02003 ato agt cg gog Ile 8cr Pro Ala aat age aat gc Asn Arg Asn Ala aat gcc gog Asr Ala Ala Cog cag Pro Gin 210 tat tgc cgg Tyr Cys Arg oaa eac gga ggc Ogg cag ata tgc apt gto ago- Gin Asn Gly Gly Arg Gin Ile Cys Set Val Ser 215 220 672 gcg gag Ogg gcg Ala Gin Arg Ala ggc ttp aat tat Sly Len Asn Tyr ato gag gog gee Ile Gin Ala Gin otg acp gop Len Thr Ala ggc ggc acg Gly Gly Thr tto ggc a Phe Sly Mrg 275 ttg poe Lien Ala 245 get aat cat tat ttg Asp Asn His Tyr lieu ttg ttc opt too eat etc Len Phe Mrg Ser Ann tIle 255 tat tat ttc apt aa Tyr Tyr Phe Set Lys eaa toe got Lys Set Ala tat gac gac ggg Tyr Asp Asp Gly 270 gcp tat ttg ggt Ala Tyr Len SlY cag tat aaa eat Gin Tyr Lys Asn Ogg cag ag Arg Gin Thr gog ggg att ttg cog ttt tat cap gtg cap ttg Ala Sly Ile Leu Pro Phe Tyr Gin Val Gin Len pgc agc gao gpo Gly Set Asp Gly pat gcp aaa ace Asp Ala Lys Thr cpg gte aac eec ogo ogo otg cop cop Mrg Val Ann Ann Mxg Mg Len Pro Pro 315 atg ctg gog cac Met Leu Ala His gtc go ptg cap Val Sly Val Gin tcc cat act tac Set His Tbr Tyr cgc oca Mrg Pro 335 1008 aac cog gga tgg caa ttt tog gtc Asn Pro Sly Trp, Gin Phe Set Val 340 ctg gee cat tao Lieu Gin His Tyr ogo caa ogo Mrg Gin Arg 350 cap gac gg Gin Asp Gly 1056 tao ogo gee Tyr Arg Gin 355 cag gat app gcg Gin Asp Arg Ala tav aet aao ggt Tyr an Ann Sly 1104 ttt tat Phe Tyr 370 gtt tcg tcg gca Val Set 5cr Ala cgt ttg ggc gaa Arp Len Gly Gin poe act gtg ttc Ala Thr Val ?he 1152 WO 01/85772 ggc ggc tgg cag Gly Gly Trp Gln 385 ggc gcg gtc aat Gly Ala Val Asn tgg gcg cag gag Trp Ala Gin Glu 420 gcg tct tat gcc Ala Ser Tyr Ala 435 gag gcg caa cgc Glu Ala Gin Arg 450 gac aag ttg tcg Asp Lys Leu Ser 465 ggc agg acg gaa Gly Arg Thr Glu gtg ttt gtg tcg Val Phe Val Ser 500 <210> 100 <211> 505 <212> PRT <213> Neisseria <400> 100 Met Leu Tyr Phe 1 Val Ser Ala Ala Ala Leu Leu Gln PCT/GB01/02003 ggc 1200 Gly 400 ggc 1248 Gly tcc 1296 Ser aca 1344 Thr cac 1392 His ttc 1440 Phe 480 gag 1488 Glu 1515 meningitidis Arg Tyr Gly Phe Leu Val Val Trp Cys Ala Ala Gly 5 10 Tyr Gly Ala Asp Ala Pro Ala Ile Leu Asp Asp Lys 25 Val Gin Arg Ser Val Ser Asp Lys Trp Ala Glu Ser 40 199 WO 01/85772 Asp Trp Lys Leu Leu Ala Asn Gly Asn Lys Leu Pro Leu Ala Lys 115 Glu LeU His 130 Ala Ala Ala 145 Phe Ala Glu Val Gly Arg Ser Gly Gly 195 Pro Gin Tyr 210 Arg Ala Glu 225 Leu Thr Ala Gly Gly Thr Phe Gly Arg 275 Ala Gly Ile 290 Asp Asn Asp Ala Pro Arg Val Val Asp Gly Asp 55 PCT/GB01/02003 Phe Leu Arg Ala Asp Arg Ala Val Ala 125 Ile Leu 140 Ser Ala Pro Val Thr Gly Ala Asn 205 Ile Cys 220 Ile Glu Phe Arg Ala Tyr Asn Ala 285 Ser Gly 300 WO 01/85772 Phe Asp Ala 305 Met Leu Ala Asn Pro Gly Tyr Arg Glu 355 Phe Tyr Val 370 Gly Gly Trp 385 Gly Ala Val Trp Ala Gin Ala Ser Tyr 435 Glu Ala Gin 450 Asp Lys Leu 465 Gly Arg Thr Val Phe Val PCT/GB01/02003 Tyr. Lys Thr Lys 310 His Gly Val 325 Trp Gin Phe 340 Gln Asp Arg Ser Ser Ala Gin Phe Val 390 Asn Asn Ala 405 Glu Trp Arg 420 Ala Arg Arg Arg Asn Arg Ser Tyr Lys 470 Glu Ser Asn 485 Ser Ala Asp 500 Arg Val Asn Asn.Arg Arg Leu Pro Pro 315 320 His Thr Tyr Arg 335 His Tyr Arg Gin 350 Gly Arg Gln Asp 365 Ser Ala Thr Val 380 Arg Glu Thr Val Gly Val Tyr Ala 415 Asn Ser Arg Val 430 Ala Ala Phe Ser 445 Leu Ala Leu Ser 460 Leu Asn Tyr Arg Arg Arg Asn Ser 495 <210> 101 <211> 1476 <212> DNA <213> Neisseria meningitidis <220> <221> CDS WO 01/85772 <222> (1)..(1476) PCT/GBO1/02003 <400> 101 stg asa tac Met Lys Tyr 1 ggc ass etc Sly Lys Leu acc gas ate Thr Glu Ile ttt gsa cac Pbs Glu His ttg gca sac Len Ala Asn gcg gac age Ala Asp Bar ttg aa gas Len Lys Gin ctg ccg etc Leu Pro Len 115 sat geg ccg Asn Ala Pro 130 tat caa ctt Tyr Gin Len 145 gts seg tgg Val Tin Trp etg cge gac Len Arg Asp atc gcg cac Ile Ala His cgc gtg etg Arg Val Len 40 sag ccc gac Lye Pro Asp 55 gge aeg ccc Sly Thr Pro 70 sag ctg egc Lye Len Arg ccg ccc ass Pro Pro Lys gac stt tgg Asp Ile Trp 120 gsa atc gta Gin le Val 135 cag cat tge Gin His Cys 150 acc gte aeg Thr Val Thr tte ate gee stg ctc gag Phe Ile Ala Met Leu Gin gtt tec ccg cat Val. Ser Pro His gcc ga Ala Giu acg cgc Thr Arg cgt gtg Arg Val atc ggg Ile Sly stt ass Ile Lye atg geg Met Ala gsa ggc Gin Sly ceg gas Pro Gin 155 ggg ceg Sly Pro 170 ggg ceg Gly Pro tat gat Tyr Asp geg. stg Ala Met eag seg Gin Thr gac gcg Asp Ala ccg sac Pro Asn 125 gsa gac Glu Asp 140 gac gtt Asp Val cac ass His Lys sat etc gge att tac cge cas eas tta ate ggc sta sac sag etg stt WO 01/83772 Asie.Lou Gly Ile. ISO atg ego tgg ctg Met AMg Trp Lou 195 ego ass etc sat Mrg Lys Lou Msn 210 tgo gac cct gee Cys Asp Pro Ala 225 ttg ago gas tao Lou 8cr Giu Tyr etg gtg aaa tgt Lou Val Lys Cys 260 ate gtg ttg ga Ile Vai'Leu Glu 275 eca tao ggo gao Pro Tyr Gly Asp 290 gtg ttt aeg gto Val Phe Thr Val. 305 tot acc tao sos Thr Tyr Tbr gcg ttg sac gas Ala Lou Msn Giu 340 ae ace gat ttt Ile Thr Asp Phe 355 gtg gtg age atg .Tyt.Arxg Gin teg cat ego 5cr His Arg aeg Thr 215 ttg Lou gee Ala aac Asn ate Ile Gly 295 ate Ile ecg Pro gte Val ccg Pro cag coo gte Pro Val gte aeg Val Thr 235 etg ego Lou Mrg 250 caa gtg Gin Val aac gee Msn Giu aac gag Asn G1u ego gae Mrg Giu 315 gsa COO Giu Pro 330 ttg cee Lou Gin ggc tgc Giy Cys Msn Lys 190 tat eag Tyr Gin 205 gtc gtg Val Vai gtt ccc Val Pro tog egg Sec rMg goc ego Ala Mrg 270 gog ttg Al a Lou 285 gao cat Asp His ocg att Pro Ile gtt ttg Val Lou cag tte Gin Pile 350 tee ego Tyr Mrg 365 PCT/GB1/02003 [tIle tte 624 LPile ggo 672 LGly ec 720 Thr 240 gsa 768 Giu *gaa 816 *Giu *ggt 864 Gly ccc 912 Pro ese 960 *His 320 gtg 1008 *Val gaa 1056 Glu gog 1104 Ala goc gga coo goo eag ego gtg stg 1152 WO 01/85772 Val Val ser Met 370 atg ggc tgc tgg Met Gly Cys Trp 385 att gtg gtg gat Ile Val Val Asp tgg gcg gta ace Trp Ala Val Thr 420 gaa aac acg ccc Glu Asn Thr Pro 435 ctt ggc ggc aaa Leu Gly Gly Lys 450 ace gac cge gaa Thr Asp Arg Glu 465 get aag att gat Ala Lys Ile Asp Gin Tyr Ala 375 ctg cgc cag Leu Arg Gin gtg gat gtg Val Asp Val atg gac ccc Met Asp Pro 425 tac etc gac Tyr Leu Asp 440 ttg gat gcg Leu Asp Ala 455 egg gtg att Arg Val Ile tgg.gag gaa Trp Glu Glu Gly His Ala 380 ttt atg tac Phe Met Tyr 395 cgc gac tgg Arg Asp Trp 410 gtg cgc gat Val Arg Asp ttc gce age Phe Ala Set ace aac aag Thr Asn Lys 460 aaa aaa gac Lys Lys Asp 475 ttg ggg ttg Leu Gly Leu 490 aaa gaa Lys Glu ace gtt Thr Val 430 ccc gtc Pro Val 445 tgg ccg Trp Pro cct gcg Pro Ala PCT/GB01/02003 Met ate 1200 Ile 400 atc 1248 Ile atg 1296 Met gga 1344 Gly gaa 1392 Glu acg 1440 Thr 480 1476 <210> 102 <211> 492 <212> PRT <213> Neisseria <400> 102 Met Lys Tyr Lys 1 Gly Lys Leu Lys Thr Glu Ile Ala Phe Glu His Pro meningitidis Asp Leu Arg Asp Phe Ile Ala Met Leu Glu Gin Gln 5 10 Arg Ile Ala His Pro Val Sex Pro His Leu Glu Met 25 Asp Arg Val Leu Arg Ala Glu Gly Pro Ala Leu Leu 40 Val Lys Pro Asp Gly Thr Arg Tyr Asp Tyr Pro Val 204 WO 01/85772 55 Leu Ala Asn Leu Phe Gly Thr Pro Glu Arg PCT/GB01/02003 Val Ala Met Gly Met Gly Ala Asp Leu Lys Leu Pro Asn Ala 130 Tyr Gin 145 Val Thr Asn Leu Met Arg Arg Lys 210 Cys Asp 225 Leu Ser Leu Val Ile Val Pro Tyr 290 Val Phe Ser Glu Leu 115 Pro Leu Trp Gly Trp 195 Leu Pro Glu Lys Leu 275 Gly Thr Val Pro 100 Leu Cys Pro Gly lle 180 Leu Asn Ala Tyr Cys 260 Glu Asp Val Ser Glu Lys Gin lle Leu 165 Tyr Ser Pro Thr Gin 245 ile Gly His Glu Lys Pro Asp Glu Gin 150 Thr Arg His Asp Ile 230 Phe Gly Val Thr Arg Leu Arg Pro Lys Ile Trp 120 Ile Val 135 His Cys Val Thr Gin Gin Arg Gly 200 Thr Pro 215 Leu Gly Ala Gly Asn Asp Tie His 280 Gly Tyr 295 Ile Thr Glu Gly 105 Ser Trp Trp Arg Leu 185 Gly Tyr Ala Leu Leu 265 Pro Tyr Met Ile Ile Met Glu Pro Gly 170 Ile Ala Pro Val Leu 250 Gln Asn Asn Arg Gly Lys Ala Gly Glu 155 Pro Gly Leu Val Thr 235 Arg Val Glu Glu Glu Gin Thr Asp Ala Pro Asn 125 Glu Asp 140 Asp Val His Lys Ile Asn Asp Tyr 205 Ala Val 220 Pro Val Gly Ser Pro Ala Thr Ala 285 Gin Asp 300 Asn Pro WO 01/85772 305 Ser Thr Tyr Thr Ala Leu Asn Glu 340 lie Thr Asp Phe 355 Val Val Ser Met 370 Met Gly Cys Trp 385 Ile Val Val Asp Trp Ala Val Thr 420 Glu Asn Thr Pro 435 Leu Gly Gly Lys 450 Thr Asp Arg Glu 465 Ala Lys Ile Asp 310 Lys Pro Phe Val Leu Pro Lys Gin 375 Phe Leu 390 Asp Val Arg Met Asp Tyr Gly Leu 455 Gly Arg 470 Ile Trp Pro Asp Pro Leu 345 Pro Glu 360 Tyr Ala Arg Gln Asp Val Asp Pro 425' Leu Asp 440 Asp Ala Val Ile Glu Glu Leu Phe 350 Arg Arg Lys Glu Val 430 Val Pro Ala PCT/GB01/02003 320 Val Glu Ala Met Ile 400 Ile Met Gly Glu Thr 480 <210> 103 <211> 1089 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1089) <400> 103 atg agt ttg aaa tgc ggc ate gtc ggt ttg ccc aac gtc ggc aaa tcc 48 206 WO 01/85772 WO 0185772PCT/GBOI/02003 Met Scr Leu Lys Cys Gly Ile Val. ly Leu Pro Asn Val- Sly Lys. 1 5 10 acc ott ttt aac gag ctg aco oaa tcg ggt ato gaa gag gon Rac tat 96 Thr Leu Phe Asn Ala Leu Thr Gin Ser Sly Ile Glu Ala Ala PAn Tyr 25 cat tto tgt aco atc gaa ccc aao gtc ggo ato gtc gaa gto coo gat 144 Pro Phe Cys Thr Ile Giu Pro Asn Val Sly Ile Val 5Th Val Pro Asp 40 cog ogt atg goc gaa ttg gca an ato gtc at cog caa aaa atg cag 192 Pro Arg Met Ala Giu Leu Ala Lys Ie Val Asn Pro Gin Lys Met Gin 55 cot gcc ato gto gas ttt gtc gat att goc ggt ttg gtt gca, ggo gag 240 Pro Ala Ile Val Glu Phe Val Asp Ile Ala Sly Leu Val Ala Sly Ala 70 75 ago aaa ggo gag ggc ttg ggo aac cag ttc ott goc sac atc ogc gaa 288 Lys Sly Giu Sly Leu Sly PAn Gin Phe Leu Ala Asn Ile Arg Giu 90 aco gat gog att gtg aat gtc gtg cgo tgo ttt gao gao gao aao atc 336 Tin Asp Ala Ile Vai Asn Vai Val Arg Cys Phe Asp Asp Asp PAn Ile 100 105 gtc oso gtt gca ggc ogo gtc gat cog att goc gao att gsa soc sto 384 Vai His Val Ala Sly Arg Val Asp Pro Ie Ala Asp Ile Giu Thr Ile 115 120 125 ggc aca gag ttg gca ott goc gac ctg gos agt gto gaa aaa goc ato 432 Sly Thr Giu Leu Ala Leu Ala Asp Leu Ala Ser Vai 5 u Lys Ala Ile 130 135 140 gtacogc gaa gaa ass ogo gco ogo tca ggo gao ass gao gog osa aag 480 Val Arg Glu Glu Lys Arg Ala Arg 5cr Sly Asp Lys Asp Ala Gin Lys 145 150 155 160 ctg gto gat ttg tgc aaa aaa ctg ctg ocg cat otg gao gsa ggc ass 528 Leu Val Asp Leu Cys Lys Lys Leu Leu Pro His Leu Asp Slu Sly Lys 165 170 175 ccc gtg cgt too ttc ggt ttg gac gcg gas gaa ogo gcg atg ctc ass 576 Pro Val Arg 5cr Phe Siy Leu Asp Ala (flu (flu Arg Ala Met Leu Lys 165 190 cog ctg ttc ctg ctg aco goc aaa cog gcg atg tat gtg ggc sac gtc 624 WO 01/95772 WO 0185772PCT/GBOI/02003 Pro Leu Phe Len Len Th-r Ala Lys Pro Ala Met Tyr Val Sly Asn Val gcc gs gac Al-a Gin Asp 210 ggt ttt gsa sac sat ccg cac ate Gly Phe Glu Ass Ass Pro His Leu 215 age ctg ass gas Arg Leu Lys Gin gag ga asa gas Ala Ala Lys Gin sac gc Asn Ala 230 ccc gta gtc Pro Val Val gtt tga gac gag Val Cys Ala Ala gag aga gaa att Glu 3cr Gin Ile gaa ttg gas gac Gin Leu Gin Asp gsa ass gac gag Gin Lys Ala Gin ttc ctc Phe Len 255 goc gas atg Ala Gin Met ggt tac ga Gly Tyr Asp 275 ttg gsa gas ccg Leu Gin Gin Pro ctg sac cga atg Len Asn Arg Leu att cgt gc Ile Arg Ala 270 qgt gtg ass Sly Val Lys ctc ttg ggg ctg Len Leu Gly Leu acc tat tta acc Thr Tyr Phe Thr gas gtc Gin Val 290 agc gcg tgg ag Arg Ala Trp Thr cac asa ggc gat His Lys Gly Asp gcc ccg as gc Ala Pro Gin Ala ggc gtg att cac Gly Val Ile His ga ttt gag Asp Phe Giu aga ggc Arg Sly 315 ata gga Len Sly 330 ttcasta aga gc Phe Ile Arg Ala 912 960 1008 1056 gtc att tat Val Ile Sex ass gas gc Lye Gin Ala ga ggc gat Asp (fly Asp 355 ta ga Tyr Asp 325 gga ass Gly Lye 340 ga ttt gtc tag Asp Phe Val 5cr gga gas gc Sly Gin Ala ass gc Lys Ala 335 stg cgt gtg Met Arg Val ggt sag gas tac gtc gtg cag Sly Lys Gin Tyr Val Val Gin 350 gta stg aa ttt Val Met His Phe ttt sac gtg Phe Ass Val 1089 <210> <211> <212> <213> 104 363 PRT Neisseris meningitidis WO 01/85772 <400> 104 Met Ser Leu 1 Thr Leu Phe Pro Phe Cys Pro Arg Met Pro Ala lle Set Lys Gly Thr Asp Ala Val His Val 115 Gly Thr Glu 130 Val Arg Glu 145 Leu Val Asp Pro Val Arg Pro Leu Phe 195 Ala Glu Asp 210 Leu Ala Ala 225 PCT/GB01/02003 Glu Set Glu Ile Ala Glu Leu Glu Asp Asp Glu Lys Ala Glu Phe Leu WO 01/85772 PCT/GB01/02003 Glu Pro Leu Gin 280 Ile His 295 Asp Phe Phe Val Arg Val Phe Leu 360 250 Gly Leu 265 Thr Tyr Lys Gly Glu Arg Ser Leu 330 Glu Gly 345 Phe Asn <210> 105 <211> 1177 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1176) <400> 105 atg gca aaa atg atg aaa tgg gcg gct Met Ala Lys Met Met Lys Trp Ala Ala 1 5 gcg gtt tgg ggc gga tgg tct tat ctg Ala Val Trp Gly Gly Trp Ser Tyr Leu 25 tat att acg gaa acg gtc agg cge ggc Tyr Ile Thr Glu Thr Val Arg Arg Gly 40 gca aca ggg gag att tcg ccg tcc aac 210 gcg gtc gag gcg Ala Val Ala Ala gag ccg cag gct Glu Pro Gln Ala agc egg acg gtt Ser Arg Thr Val tcg gte ggc gcg WO 01/85772 Ala Thr .Giy gos tog ggg Ala 3cr Sly aa sag ggc Lys Lye Gly acg oto aat Thr Leu Asn gtg tcg gos Val Bar Ala 115 wag gog gog Gin Ala Ala .130 ago gos wag 8cr Ala Gin 145 sag gct cta Lys Ala Leu gas ttg ggo Glu Leu Gly gcg att ctc Ala Ile LeU 195 cog acg att Pro Thr Ile 210 wag att goc Gin Ile Ala 225 Ile Att Ile ttg Leu gas Giu att Ile tgg Trp gcg Al. a aga Arg 165 acg Thr gas Glu waa Gin Gly 5cr Val Gly ctc ggg oaa Leu Gly Gin acc tog wag Thr 3cr Gin tat cag gog Tyr Gin Ala 110 sag ass tat Lye Lye Tyr 125 asa gas gat Lye .Giu Asp 140 sat gtt gee Asn Val Ala aat aoo gc Asn Thr Ala gao ggo sog Asp Giy Thr 190 gog gcg wag Ala Ala Gin 205 stg ttg aao Met Leu Msn 220 gog ggg cag Ala Gly Gin PCT/GBO1/02003 gtt 240 Val sat 288 Asn otg 336 Leu cgt 384 gsa 432 Giu otg 480 Leu 160 tog 528 3cr gtg 576 Val aog 624 Thr sty 672 Met stt 720 Ile 240 tog ttt sog att ttg teo gas cog gat sog ocg att sag gog asg oto WO 01185772 PCT/GB01/02003 Ser Phe Thr Ile Leu Ser Glu Pro Asp Thr Pro Ile Lys Ala Lys Leu 245 250 255 gac age gtc gac ccc ggg ctg ace aeg atg teg tog ggc ggc tac aac 816 Asp Ser Val Asp Pro Gly Leu Thr Thr Met Ser Ser Gly Gly Tyr Asn 260 265 270 age agt acg gat acg get too aat gcg gte tac tat tat gcC cgt tog 864 Ser Ser Thr Asp Thr Ala Ser Asn Ala Val Tyr Tyr Tyr Ala Arg Set 275 280 285 ttt gtg cog aat ccg gac ggc aaa ctc gcc acg ggg atg acg acg cag 912 Phe Val Pro Asn Pro Asp Gly Lys Leu Ala Thr Gly Met Thr Thr Gln 290 295 300 aat acg gtt gaa ate gac ggt gtg aaa aat gtg ctg att att ccg tcg 960 Asn Thr Val Glu Ile Asp Gly Val Lys Asn Val Leu Ile Ile Pro Ser 305 310 315. 320 ctg acc gtg aaa aat cgc ggc ggc agg gcg ttt gtg cgo gtg ttg ggt 1008 Leu Thr Val Lys Asn Arg Gly Gly Arg Ala Phe Val Arg Val Leu Gly 325 330 335 gca gac ggc aag gcg gcg gaa cge gaa .ate egg ace ggt atg aga gac 1056 Ala Asp Gly Lys Ala Ala Glu Arg Glu Ile Arg Thr Gly Met Arg Asp 340 345 350 agt atg aat ace gaa gta aaa age ggg ttg aaa gag ggg gac aaa gtg 1104 Ser Met Asn Thr Glu Val Lys Ser Gly Leu Lys Glu Gly Asp Lys Val 355 360 365 gtc atc too gaa ata ace gcc gcc gag cag cag gaa age ggc gaa cgc 1152 Val Ile Ser Glu Ile Thr Ala Ala Glu Gln Gln Glu Ser Gly Glu Arg 370 375 380 gec eta ggc ggc cog cog ogo cga t 1177 Ala Leu Gly Gly Pro Pro Arg Arg 385 390 <210> 106 <211> 392 <212> PRT <213> Neisseria meningitidis <400> 106 Met Ala Lys Met Met Lys Trp Ala Ala Val Ala Ala Val Ala Ala Ala 1 5 10 212 WO 01/85772 Ala Val Trp Gly PCT/GB01/02003 Ala Gly Trp Ser Tyr Leu Lys Pro Glu Pro Gin Ala Tyr lie Ala Thr Ala Set Lys Lys Thr Leu Val Ser Gin Ala 130 Ser Ala 145 Lys Ala Glu Leu Ala Ile Pro Thr 210 Gin Ile 225 Ser Phe Asp Ser Glu Glu Gin Asp Thr 100 Gin Leu Asp lle Tyr 180 Val Val Glu Ile Asp 260 Gly Asp Asn Leu Tyr Val Ile Asn Leu Glu 105 Ser Ala Ala Thr Ala Lys lie Ser 170 Ala Thr 185 Thr Val Leu Asp Lys Val Asp Thr 250 Thr Met 265 Thr Val Gly Ala Gin Gin Gin Thr Ala Lys 110 Tyr Lys Asp Leu Ala Glu Ala Glu 175 Thr Val 190 Gin Ser Asn Lys Gin Asp Ala Lys 255 Gly Tyr 270 WO 01/85772 Ser Ser Thr 275 Phe Val Pro 290 Asn Thr Val 305 Leu Thr Val Ala Asp Gly Ser Met Asn 355 Val Ile Ser 370 Ala Leu Gly 385 Thr Ala Pro Asp Ile Asp 310 Asn Arg 325 Ala Ala Glu Val Ile Thr Pro Pro 390 Ser Asn Ala 280 Gly Lys Leu 295 Gly Val Lys Gly Gly Arg Glu Arg Glu 345 Lys Ser Gly 360 Ala Ala Glu 375 Arg Arg PCT/GB01/02003 Ser Gln Ser 320 Gly Asp Val Arg <210> 107 <211> 1185 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1185) <400> 107 atg aca gag gac gaa cgt ttc Met Thr Glu Asp Glu Arg Phe 1 5 tat atc ggc gcg gaa agt ttc Tyr Ile Gly Ala Glu Ser Phe I gcg caa sat gcc ctg tcc gca Ala Gln Asn Ala Leu Ser Ala gcg la :tg Leu ccg ?ro 40 214 tgg ctg caa ttg gcg ttt acg ccc Trp Leu Gln Leu Ala Phe Thr Pro 10 ctg ctg atg cgc cgt ttc ggc agc Leu Leu Met Arg Arg Phe Gly Ser 25 gcg gaa cag gtg gcg gca ctg ata Ala Glu Gln Val Ala Ala Leu Ile WO 01/85772 egg coo aaa cag Arg Hlis Lys Gin otg gog ogg cag Lou Ala Arg Gin gga tgo ogo ctg Gly Cys Arg Len PCT/GBO1/02003 gog ott gag got tgg ogo aat gcg gas asa ogo got 192 Ala Lou Gin Ala Trp Arg Asn Ala Giu Lys Arg Ala 55 cag Gin ctg Lou cog Pro 130 aa Gin gcc Ala ggg Gly tat Tyr aog Thr 210 qao gaa gog Gin Ala ott cag Lou GIn cog ccg Pro Pro toc gc 5cr Ala 120 att gc Ile Ala 135 gtg tog Val 5cr otg gag Lou Glu cgc att Arg Ile aaa gga LYS Gly 200 ggo oat Gly Asn 215 gaa tgg gaa Giu Trp Glu gat tto ccc Asp PMe Pro ttt ttg ego Phe Leu Arg gto ggc ago Val tfly 125 tto ggo aag Phxe Gly ILys 140 got too ggt Ala 5cr Gly 155 ggo ggo cc Gly Gly Thr cog too oat Pro 8cr Asn gte ago gag Val. 5cr Gin 205 ago ago aao Arg Arg Asn 220 cg otg gtg gtt gaa goc goo ttg gas too Ala Ala Len 8cr Gin Val Thr Lou Val Val Glu Ala Ala Leu Gin 225 230 235 240 WO 01/85772 ggt tag ctg PCTIGBOI/02003 att acc gc ggc ctg gag gca gas atg ggg cga gas gtg 768 Gly Ser Lou Ile Thr Ala Gly Lou Ala Ala Glu Met Gly Arg Glu Val 250 gae ast cag cac Asp Asn Pro His 265 aaa ttg gtg gsa Lye Lou Val Glu 280 ttg caa aat acg Leu Gin Asn Thr gac acg gga cgc Asp Thr Gly Arg 315 gca aaa atg cog Ala Lye Met Pro 330 ata ctg gat aaa Ile Lou Asp Lye 345 gga cag ttg gct Gly Gln.Leu Ala 360 ttg gas ttg gac Leu Giu Lou Asp 255 816 864 912 960 1008 1056 1104 1152 stg ccc agc gga aga tac cag ogt etc cga act Met Pro Sor Gly Axg Tyr Gin Arg Ile Arg Thr 385 390 395 <210> 108 <211> 395 <212> PRT <213> Neisseria moningitidis <400> 108 1185 WO 01/85772 Met Thr Glu 1 Tyr Ile Gly Ala Gin Asn Arg His Lys Leu Ala Arg Gly Cys Arg Thr Gin Gly Arg Leu Leu 115 Thr Pro Gin 130 Gly Gln Asn 145 Ala Ala His Trp Gly Thr Ala Tyr Glu 195 Gly Thr Arg 210 Ala Ala Leu 225 Gly Ser Leu PCT/GB01/02003 Asp Glu Arg Phe Ala Trp Leu Gin Leu Ala Phe Thr Pro 5 10 Leu Leu Pro Ala 40 Ala Trp Ala Ala Gin Asp Pro Val 105 Ala Ala 120 Ala Lys Ser Gly Glu Ala Ile Tyr 185 Gly Leu 200 Asn Phe Leu Val Leu Ala Arg Arg Val Ala Ala Glu Trp Glu Phe Pro Leu Arg Gly Ser 125 Gly Lys 140 Ser Gly Gly Thr Ser Asn Ser Glu 205 Arg Asn 220 Ala Ala Met Gly Gly Leu Arg Arg Met Asn His Leu Asp Ala 175 Asn Pro Leu Glu Glu 255 WO 01/85772 Met Ala Val Lys Leu Ile 275 Leu Asn Glu 290 Ser Ile Asn 305 Ala Tyr Ala Gly Gly Thr Ile His Pro 355 Leu Tyr Ala 370 Gly Ser Ile Asp Gly Ala Pro Gly Leu 295 Asp Thr Pro 310 Pro Pro Ala 325 Val Gly Gly Val Leu Ala Leu Leu Glu 375 PCT/GB01/02003 His Ile Tyr Thr 320 Ala Pro Asp Ala Met Pro Ser Gly Arg Tyr Gin Arg Ile Arg Thr <210> 109 <211> 1257 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1257) <400> 109 atg aaa cag acc gtc ctc aaa aat aac ctg caa aac ctg ctt gaa ago Met Lys Gln Thr Val Leu Lys Asn Asn Leu Gin Asn Leu Leu Glu Ser 1 5 10 gca gaa aat atc ctg ctg ctt caa ggc cct gtc ggc gat ttt ttt ctg Ala Glu Asn Ile Leu Leu Leu Gin Gly Pro Val Gly Asp Phe Phe Leu 25 cgc ctt gcc gac tgg ctg act gca aac ggc aaa acc gta cat aaa ttc 218 WO 01/85772 Arg Leu Ala aac ttt sat Asn Phe Asri aoc gtt gtt Thr Val Val gaa tao atc Glu Tyr Ile aca ogc cot Thr Arg Pro goc agt ttc Ala So: Phe 115 acc tta gas Tb: Leu Glu 130 gc gac ttt Ala Asp Phe 145 ass geg oca Lys Ala Pro got ato ogt Ala le Arg gac tac ate Asp Tyr Ile 195 tgg tog cto Trp Se: LeU 210 Trp Leu ggo gao Gly Asp aac gao Asn Asp 70 osa cat Gin His cac *gto His Val gcg ttt Ala Phe gao ggo Asp Gl y ott gaa Leu Giu 150 Cog gta Pro Val 165 tat ato Tyr Ile cac cgc His Arg atc ctc Ile Leu PCT/GBO1/02003 Phe cat 192 His ona 240 Gin gao 288 Asp oaa 336 Gin ato 384 Ile ogt 432 Arg tat 480 Tyr 160 aso 528 Asn coo 576 Pro ocg 624 Pro ato 672 Ile cas atc gcc asa cgt gtg gas gca ggc asa tao ggc sag ttt ttt stt WO 01/85772 Gin Ile Ala 225 gtt ccc ttg Val Pro Leu ttt ccc agc Phe Pro Ser gaa cac gcg Glu His Ala 275 gac ege ggc Asp Arg Gly 290 gaa cac ccc Glu His Pro 305 ctg ccc gtt Leu Pro Val PCT/GB01/02003 e Ile 240 gac 768 i Asp t gcc 816 Ala atg 864 Met aaa 912 i Lys ccc 960 Pro 320 agc 1008 I Ser ctc 1056 Leu ttg 1104 Leu cat 1152 His ttc 1200 1 Phe 400 aca 1248 Thr 1257 WO 01/85772 Pro Thr Thr <210> 110 <211> 419 <212> PRT <213> Neisseria PCT/GB01/02003 meningitidis <400> 110 Met Lys Gin 1 Ala Glu Asn Arg Leu Ala Asn Phe Asn Thr Val Val Glu Tyr Ile Thr Arg Pro Ala Ser Phe 115 Thr Leu Glu 130 Ala Asp Phe 145 Lys Ala Pro Ala ile Arg Asn Asn Leu Gln Gly Pro Ala Asn Gly Tyr Phe Tyr Tyr Asp Ala Ile Gin Ala Ala Lys Arg 105 Glu Gly Tyr 120 Asn Ala Phe Phe Pro Lys Gly Gly Phe 170 Leu Phe Arg 185 Pro Asn Ala 200 221 Asp Tyr Ile His His Arg Ala 195 WO 01/85772 Trp Ser Leu 210 Ser Ile Leu Lys Arg Leu Asn Tyr Tyr Ile Glu Asp 215 220 Gin 225 Val Phe Glu Asp Glu 305 Leu Thr Gly Ala Ala 385 Arg Val 230 Val Phe 245 Arg Ser Ala Asp Ile Asp Leu Lys 310 Leu Arg 325 Ser Gly Tyr Asp Asn His Tyr His 390 Glu Ala Asn Asp Phe Leu Tht Asn 280 Tyr Trp 295 Gly Arg His Gly Leu Ile Ile Pro 360 Pro Thr 375 Leu Asn Phe Phe His Cys 255 Ser Phe 270 His Pro Phe Ile Asp Val Ile Asn 335 Lys Val 350 Asn Thr Leu Phe Gly Asn PCT/GB01/02003 Ile Ile 240 Asp Ala Met Lys Pro 320 Ser Leu Leu His Phe 400 Tyr Ser Gin Val Phe Phe Pro Asn Lys Asn Thr Ser Asp Ser Ser Thr 405 410 415 Pro Thr Thr <210> 111 <211> 1674 <212> DNA <213> Neisseria meningitidis WO 01/85772 <220> <221> CDS <222> (1674) PCT/GBO1/02003 <400> 111 atg agt ti Met 5cr P) 1 cog att g Pro Ile G. gag act ti Glu Pro Tj tat ctg C( Lys Leu Pi ccg aot C; Pro Thr Pi gac goa tt Asp Ala Lf Cot tot tt Pro Oar Le ggc tat gc Gly Tyr Al 12 gga gat tt Gly Asp P1 130 otc gao ta Leu ASP A2 145 Cgc gtg at Arg Val Lc gaa ate Glu Ile aag ctg Lys Leu goc aaa Ala Lys agg otg Arg Lieu tat ace Lys Thr tat gao Lys Asp ggo gtg Gly Val 105 atg gaa Met Giu 120 gcg got Ala Ala ggo tao Gly Asn toe acc Ser Thr gtt gao Val Asp gao gaa Ala Gilu aca goc Thr Ala ato ggt Ile Gly got ttg Al a Lou gog got Ala Ala 110 otg cac Leu His 125 ott gc Leu Ala ato gao Ile Asp atg ogo Met Arg aac att Asn Ile gag ttc Ala Pita ata tao Ile Asn ttg gcg Leu Ala ogo gag Arg Giu Giy Gly tto aca Phe Thr gcg atg Ala Met ccc aaa Pro Lys 160 gtc gat ttg aao gac ago cag ttg ago Arg Arg Val. Val Asp Met Asn 223 Asp Arg Gin Lieu Arg WO 01/85772 PCT/GB01/02003 ggc gtg Gly Val atg gcg Met Ala 205 ttg ggc Leu Gly 220 gcc aaa Ala Lys gcg att Ala ile gta cac Val His acc gca Thr Ala 285 gca ggc Ala Gly 300 tgc cgc Cys Arg gtc cgc Val Arg gaa gaa Glu Glu cac att His Ile 576 624 672 720 768 816 864 912 960 1008 1056 1104 WO 01/85772 355 PCTIGBOIIO2003 aaa Lys tee 3cr 385 cc His ggC Gly ace Thr aa Lys tte Phe 465 ttg Leu gac Asp ege Arg ggc ely aae Lys aae gta Asn Val 370 gac tee Asp 5cr ggc gtt Gly Val gge gag Gly Mla aat acc Asn PAn 435 ate cgt le Mrg 450 agc gcg Ser Ala gac ace Asp Lys aac gce an Mla gge ae Gly Ile 525 aac ctg an Met 530 ae get Ile Asp tte gga ctg Phe Sly Lou gte gtt gag Val yal Mla atg att gac Met Ile Giu 395 gea gtg tgg Glu Val Trp 410 gte gte aac Val Val Pan 425 get gte gag Asp Val Glu gtg tee ggc Val Tyr Gly ate get tee Ile Ala 5cr 475 gag aca cc Ala Lys Tin 490 ccc gee gee Pro Glu Asp 505 gee ggt tte Ale Gly Phe etg ce ace Lou Pro Lys gtg ett c 365 etc aac Leu PAn 380 aac gcc Lys Ala ggc aaa Gly Lys gcc att Mla Ile ttg gge Lou Gly 445 gag gaa Ale Glu 460 etg gee Leu Giu ae tee Gin Tyr tte cgc Phe Arg ate gte Ile Val 525 gtt cog Val Pro 540 gge ttg 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1674 Gly Val 225 Ile His Gly Leu Phe WO 01/85772 PCT/GB01/02003 545 550 555 <210> 112 <211> 558 <212> PRT <213> Neisseria meningitidis <400> 112 Met Ser Phe Lys Thr Asp Ala Glu Ile Ala Gin Ser Ser Thr Met Arg 1 5 10 Pro Ile Gly Glu Ile Ala Ala Lys Leu Gly Leu Asn Val Asp Asn Ile 25 Glu Pro Tyr Gly His Tyr Lys Ala Lys Ile Asn Pro Ala Glu Ala Phe 40 Lys Leu Pro Gin Lys Gin Gly Arg Leu Ile Leu Val Thr Ala Ile Asn 55 Pro Thr Pro Ala Gly Glu Gly Lys Thr Thr Val Thr Ile Gly Leu Ala 70 75 Asp Ala Leu Arg His Ile Gly Lys Asp Ser Val Ile Ala Leu Arg Glu 90 Pro Set Leu Gly Pro Val Phe Gly Val Lys Gly Gly Ala Ala Gly Gly 100 105 110 Gly Tyr Ala Gin Val Leu Pro Met Glu Asp Ile Asn Leu His Phe Thr 115 120 125 Gly Asp Phe His Ala Ile Gly Ala Ala Asn Asn Leu Leu Ala Ala Met 130 135 140 Leu Asp Asn His Ile Tyr Gin Gly Asn Glu Leu Asn Ile Asp Pro Lys 145 150 155 160 Arg Val Leu Trp Arg Arg Val Val Asp Met Asn Asp Arg Gin Leu Arg 165 170 175 Asn Ile Ile Asp Gly Met Gly Lys Pro Val Asp Gly Val Met Arg Pro 180 185 190 Asp Gly Phe Asp Ile Thr Val Ala Ser Glu Val Met Ala Val Phe Cys 195 200 205 WO 01/85772 PCT/GB01/02003 Ile Ser Asp Leu Lys Glu Arg Leu Gly Asn Ile Leu 215 220 Leu Ala 210 Val Ala 225 Ala Asn Leu Val Phe Ala Ala Lys 290 Asp Leu 305 Leu Lys Tyr Asn Ala Leu Lys Asn 370 Ser Asp 385 His Gly Gly Gly Thr Asn Lys Ile 450 Lys Asp Tyr Ala Gly Ala Gin Thr 260 Asn Ile 275 His Leu Gly Ala Pro Asp Gly Gly 340 Glu Lys 355 Val Phe Ser Asp Val Glu Ala Asp 420 Asn Phe 435 Arg Ala Gly Ala Gly Gly Tyr 295 Phe Val Arg Pro Pro 375 Leu Leu Arg Ala Gln 455 Tyr Ala 235 Asp Ala Phe Val Val Thr Glu Ala 300 Lys Cys 315 Thr Val Gly Glu Lys His Ala Leu 380 Glu Lys 395 Trp Gly Asn Ala Glu Leu Gly Ala 460 WO 01/85772 Phe Ser Ala Glu 465 Leu Asp Lys Met Asp Asn Ala Lys 500 Arg Gly Ile Thr 515 Gly Asn Met Met 530 Lys Ile Asp Val 545 PCT/GB01/02003 Gly 480 Ser Val cys Glu <210> 113 <211> 475 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 113 atg gaa cac cta ttt gag gaa tgg ttg Met Glu His Leu Phe Glu Glu Trp Leu 1 tca gac ggc ate ggc ctg ccg atg age Ser Asp Gly Ile Gly Leu Pro Met Ser ctg act gcc gca ttg cgc gcc ttg ccg Leu Thr Ala Ala Leu Arg Ala Leu Pro ctg aaa ctg ggc gaa ttg gaa acg gaa Leu Lys Leu Gly Glu Leu Glu Thr Glu gaa gtt ttg ttg aag ctg gac ggt atc Glu Val Leu Leu Lys Leu Asp Gly Ile 228 WO 01/85772 gaa tgc cgt. atc Gin Cys Arg Ile acg cgt act ttg Thr Arg Pro Len 100 cgt tag gag ttt Arg Sex Ala Phe 115 ttt gac gct cgg Phe Ala Ala Mrg 130 ctg aca gag tat Len Thr Gin Tyr 145 <210> 114 <211> 158 <212> PRT <213> Neisseria <400> 114 Met Gin His Len 1. 3cr Asp Gly Ile Len Thr Ala Ala Len Lys Leu Gly Gin Val Len Len Glu Cys Arg Ile 'Vbx Ag Pro Len ggt tag gag ttt Gly Sex Ala Phe ggc gaa cgt ctg Gly Gin Mrg Len gag ttt gcc gtt Gin Phe Ala Val 120 agt tag agg ttt Mrg 3cr Mxg Phe 135 ttt ctg ccc gaa Phe Len Pro Gin 150 meningitidis Phe Gin Gin Trp 5 Gly Len Pro Met Len Arzg Ala Len Gin Len Gin Thr 1 Lys Len Asp Gly 70 Gly Ser Ala Phe Gly Gin Mrg Len 1 229 75 aa aaa Gin Asn 90 aaa gc Gin Ala ggc gaa Gly Gin cat cac His His aaa cgc Lys Mrg 155 Pro Asp Mrg Len His Len Cys Gly Pro Val Gin Asn Gin Ala att ttg gac Ile Len Asp PCT/GBO1/02003 tga ggc 288 Cys Gly ggg gag 336 (fly Ala cgg tac 384 Arg Tyr atg ctg 432 Met Len t 475 WO 01/85772 WO 0185772PCTIGBOIIO2003 14D0 105 110 3cr Ala Phe Gin Phe Ala Val Phe Gly Gin Gly Cys ely Arg Tyr 115 120 125 AlIa Ala Arg Arg Ser Arg Phe Ser His His ely Gin Gin Met Len 130 135 140 Thr Gin Tyr Phe Leu Pro Gin Len Lys Arg Phe Ile ely 150 155 <210> 115 <211> 1476 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1476) <400> 115 atg aaa tao ana gao otg ogo Met Lys Tyr Lys Asp Len Arg 1 5 ggo aaa ctc aaa ogo ate gcg Sly Lys Len Lys Arg Ile Ala 1 acc gaa ato goc gao ogc gtg Thr Gi Ile Ala Asp Arg Val ttt gan cac ca gtt aag 0004 Phe Gin His Pro Val Lys Pro ttg goa aao otg ttc ggc aog Len Ala Aan Len Phe Sly Thr 70 gog gac ago gtg too aag otg Ala Asp Ser Val Ser Lys Leu J gag cag cag Gin Gin Gin ttg gaa atg Leu Gin Met gog ttg ttg Ala Len Len tat 000 gtg Tyr Pro Val ggo atg ggo ely Met ely otg gog tat Len Ala Tyr ttg aaa gaa 000 gas cog 000 aa ggc att aaa gao gog ttt too aa WO 01/85772 Len Lye Gin Pro .100 ctg ccg oto ttg Leu Pro Leu Len 115 aat gcg cog tgt Asn Ala Pro Cys 130 tat caa ott cog Tyr Gin Leu Pro 145 gta acg tgg ggc Vai Thr Tip Gly aat ctc ggc att Asn Len Gy Ile 180 atg cgc tgg otg Met Arg Tip Len 195 ogo aaa ctc aat Arg Lye Leu Asn 210 tgc gao cct gc Cys Asp Pro Mla 225 ttg ago gaa tac Len Ser Gin Tyr ctg gtg aaa tgt Len Val Lye Cys 260 atc gtg ttg gaa Ile Val Len Gin 275 cca tac ggc gac Gin Pro aaa gac Lye Asp cag gaa Gin Gin att cag Ile Gin 150 ttg ac Len Thr 165 tao ogo Tyr Aig tcg cat Her His Pro att Ile atc Ile 135 cat His gjtc Val caa Gin cgc Arg acg Thr 215 ttg Len gcc Ala aac Aen atc Ile ggc Lye tgg Tip 120 gta Val *tgc Cys acg Thr caa Gin Gly 200 cog Pro ggc Gly gga Gly gat Asp cat His 280 tat Gly 105 ago Her tgg Tip tgg Trp c Arg tta Leu 185 ggo Giy tat Tyr gcg Ala ctg Len ttg Len 265 Pro tac PCTIGBOIIO2003 Her Lye gtg aaa 384 Val Lys gat ttg 432 Asp Len cog otg 480 Pro Len 160 ogo on 528 Arg Gin 175 otg att 576 Le Ile gaa ttc 624 Gin Phe ctc ggc 672 Len Gly gat acc 720 Asp Thi 240 aog gaa 768 Thr Gin 255 goa gaa 816 Mla Gin gaa ggt 864 Gin Gly ttc 000 912 WO 01/85772 WO 0185772PCTIGBO1/02003 Pro Tyr Gly Asp 290 His Thr Gly Tyr Tyr Asn Gia Gin Asp His Phe Pro ttt aeg gtc gaa ego Phe Thr Val Giu, Arg atc acc atg ogo gaa aac cog Ile Thr Met Arg Giu Asn Pro att tao cac Ile Tyr His 320 tot ace tao aca 3cr Thr Tyr Thr gog ttg aac gaa Ala Leu Asn Giu 340 aa c og coo gat gee coo gcc gtt ttg Lys Pro Pro Asp Gin Pro Ala Val Len 330 ggo gtg Gly Vai .335 1008 gtg ttc gta Val Phe Val cog ctt Pro Lou 345 000 gee Pro Gin 360 ttg ca aag cag Leu Gin Lys Gin tto c00 gee Phe Pro Gin 350 1056 atc aoc get ttt tac otg cog Ile Thr Asp Phe Tyr Leu Pro 355 gtg gtg ago atg aan aaa cag Yai Val 5cr Met Lys Lys Gin 370 375 ggc tgo tcc Gly Cys 5cr tao ego atg gog Tyr Arg Met Ala 365 eag 090 gtg atg Lys Arg Val Met 1104 1152 tao gcc gga cc Tyr Ala Gly His 990 tgo tgg tog Giy Cys Trp, 3cr atg ogo cag ttt atg tao aco aaa tto Len Arg Gin Phe Met Tyr Thr Lys Phe 395 atc Ile 400 i2 00 1248 att gtg gtg gat Ile Val Val Asp gat gtg gat gtg 090 gao tgg ace ga Asp Val Asp Val Arg Asp Trp Lys Gin 410 gtc etc Val Ile 415 tgg 909 gte Trp Ala Val acg cgc atg gac ccc gtg cgo get Thr Arg Met Asp Pro Val Arg Asp 425 aco gtt ttg atg Thr Val Leu Met 430 1296 gaa aao aeg 000 Gin Asn Thr Pro ato gac tao oto Ile Asp Tyr Len gao tto goc ago coo gto ago gge Asp Phe Ala Ser Pro Val 5cr Gly 445 1344 ott ggC Lou Giy 450 990 aan atg ggt Gly LYS Met Gly get gcg aoo aec eag Asp Ala Thr Asn Lys 460 tgg oog ggo ga Trp Pro (fly Gll 1392 gao ogo gee tgg Asp Arg Giu Trp 099 gtg ctt aaa ana gao cot gog gtt Arg Val Ile Lys Lys Asp Pro Ala Val 475 1440 got aeg att get gag Ott tgg gag gee ttg ggg ttg 17 1476 WO 01/85772 Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490 PCT/GB01/02003 <210> 116 <211> 492 <212> PRT <213> Neisseria <400> 116 Met Lys Tyr Lys 1 Gly Lys Leu Lys Thr Glu Ile Ala Phe Glu His Pro Leu Ala Asn Leu Ala Asp Ser Val Leu Lys Glu Pro 100 Leu Pro Leu Leu 115 Asn Ala Pro Cys 130 Tyr Gin Leu Pro 145 Val Thr Trp Gly Asn Leu Gly lie 180 Met Arg Trp Leu 195 meningitidis Asp Leu Arg Asp Phe Ile Ala Met Leu Glu Gin Gin 10 WO 01/85772 Arg Lys Leu Asn 210 Cys Asp Pro Ala 225 Leu Ser Glu Tyr Leu Val Lys Cys 260 Ile Val Leu Glu 275 Pro Tyr Gly Asp 290 Val Phe Thr Val 305 Ser Thr Tyr Thr Ala Leu Asn Glu 340 Ile Thr Asp Phe 355 Val Val Ser Met 370 Met Gly Cys Trp 385 Ile Val Val Asp Trp Ala Val Thr 420 Glu Asn Thr Pro 435 Leu Gly Gly Lys 450 Pro Asp Thr Pro Tyr Pro Val Ala Val Val Leu 215 220 PCT/GB01/02003 Gly Gly Ala Gly Leu Asp Leu 265 His Pro 280 Tyr Tyr Thr Met Pro Asp Pro Leu 345 Pro Glu 360 Tyr Ala Arg Gin Asp Val Asp Pro 425 Leu Asp 440 Asp Ala 234 Pro Val Gly Ser Pro Ala Thr Ala 285 Gin Asp 300 Asn Pro Ala Val Lys Gin Ser Tyr 365 Ala Lys 380 Tyr Thr Trp Lys Asp Thr Ser Pro 445 Lys Trp 460 WO 01/85772 PCTIGB01/02003 Thr Asp Arg Glu Trp Gly Arg Val Ile Lys Lys Asp Pro Ala Val Thr 465 470 475 480 Ala Lys Ile Asp Glu Ile Trp Glu Glu Leu Gly Leu 485 490 <210> 117 <211> 1515 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1515) <400> 117 atg ttg tat ttc aga tac ggt ttt ttg gtt gtt tgg tgt gcg gca ggt 48 Met Leu Tyr Phe Arg Tyr Gly Phe Leu Val Val Trp Cys Ala Ala Gly 1 5 10 gtt tot gcc gcc tat ggg gcg gat gcg ccc gcg att ttg gat gac aag 96 Val Ser Ala Ala Tyr Gly Ala Asp Ala Pro Ala Ile Leu Asp Asp Lys 25 gca ttg ttg cag gtg cag cgg tcg gtg tog gat aag tgg gcg gaa tcg 144 Ala Leu Leu Gin Val Gin Arg Ser Val Ser Asp Lys Trp Ala Glu Set 40 gat tgg aaa gtt gac aat gat gcc cog cgc gtg gtt gac ggg gat ttt 192 Asp Trp Lys Val Asp Asn Asp Ala Pro Arg Val Val Asp Gly Asp Phe 55 ttg ttg gcg cat ccg aaa atg ttg gaa cat agt ttg cgc gac gtg ctc 240 Leu Leu Ala His Pro Lys Met Leu Glu His Ser Leu Arg Asp Val Leu 70 75 aac ggc aat cag gcg gat ttg atc got tcg ttg gcg gat ttg tat gcc 288 Asn Gly Asn Gin Ala Asp Leu Ile Ala Ser Leu Ala Asp Leu Tyr Ala 90 aag ctg ccg gat tat gac gcg gtt ttg tac ggc agg gcg cgg get ttg 336 Lye Leu Pro Asp Tyr Asp Ala Val Leu Tyr Gly Arg Ala Arg Ala Leu 100 105 110 ctg gcg aaa ttg gcg gga agg ccg gcg gag gcg gtg gcg cgg tat cgg 384 235 WOOI1/85772 .PCTIGBO1IO2003 Leu Ala Lys Leu Ala Sly Arg Pro Ala Glu Ala Val Ala Arg Tyr Arg 115 120 125 gaa ctg sac ggg gaa aat gog gca gac gag cgg att ttg. otg get ttg 432 Glu Leu His Gly Glu Asn Ala Ala Asp Gin Arg Ile Leu Leu Asp Leu 130 135 140 gcg gog gog gag ttt gac gat ttc ogg otg aag tog gsa gaa agg cat 480 Ala Ala Ala Gin Phe Asp Asp Phe Arg Len Lys 5cr Ala Glu Arg His 145 150 155 160 ttt gco gag gog gea eaa ttg gat ttg cog gog cog gtt ttg gaa aat 528 Phe Ala Gin Ala Giu Lys Len Asp Leu Pro Ala Pro Val Len Slu Asn 165 170 175 gtg ggg ogt ttt cgg aaa aaa gog gag ggg ctg acg ggc tgg cgt ttt 576 Val Sly Arg Phe Arg Lys Lys Ala Gin Sly Len Thr Gly Trp Arg Phe 180 185 190 tog ggo ggc ae agt cog gog gtc eat age eat gcc aat aat goo gog 624 Sly Sly Ile 5cr Pro Ala Val Asn Arg Asn Ala Asn Asn Ala Ala 195 200 205 cog oag tat tgc cgg caa aao gga ggc cgg sag ate tgc agt gtc agc 672 Pro Gln Tyr Cys Arg Gin Asn Gly Gly Arg Gin Ile Cys Set Val 8cr 210 215 220 ogg gog gag cgg gcg gca ggo ttg eat tat gaa ate gag gog gaa aaa 720 Arg Ala Gin Arg Ala Ala Sly Len Asn Tyr Gin Ile Gin Ala G1w Lys 225 230 235 240 otg aog gog ttg gca gat aat oat tat ttg ttg tto ogt too aat ato 768 Lou Thr Ala Len Ala Asp Asn His Tyr Len Len Phe Arg 5cr Asn Ile 245 250 255 ggo ggc acg agc tat tat ttc agt aaa aaa toa got tat gac gao ggg 816 Sly Gly Thr 3cr Tyr Tyr Phe Ser Lys Lys Ser Ala Tyr Asp Asp Sly 260 265 270 ttc ggo aga gog tat ttg ggt tgg sag tat aaa aat goa ogg ceg aog 864 Phe Sly Arg Ala Tyr Len Sly Trp Gin Tyr Lys Asn Ala Arg Gin Thr 275 280 285 gcg ggg att ttg cog ttt tat cag gtg oag ttg tcg ggo ago gao ggo 912 Ala Gly Ile Len Pro Phe Tyr S-in Val Sin Len 5cr Sly 3cr Asp sly 290 295 300 ttt gat gog aea aca aaaeogg gta eec aac ogo ogc ctg cog cog tat 960 WO 61/85772 WO 0185772PCT/GBO1/02003 Asp Ala Lys Thr Lys 310 gga gtc Gly Val 325 Arg Val Asn Asn Arg 315 Arg Lcu Pro Pro atg ctg gcg cac Met Lieu Ala His ggc gtg cag ttg tcc cat act tac Gly Val Gin Lcu 5cr His Thr Tyr 330 cgc cca Arg Pro 335 1008 aagp ccg gga tgg caa ttt tcg gtc gcg ctg gaa. Ann Pro Gly Trp Gin Phe Sc: VaJ. Ala Lieu Giu cat tac cgc caa cgc His Tyr Arg Gin Arg 350 ggt cgg cag gac ggg Gly Arg Gin Asp Gly 365 1056 tac cgc gaa Tyr Arg Giu 355 cag gat agg gcg gaa tac eat aac Gin Asp Arg Ala Glu Tyr Asn An 360 1104 ttt tat Phe Tyr 370 gtt tcg tcg gca Val 3cr Sc: Ala cgt ttg ggc gee tcg gca act gtg ttc Arg Lieu Gly Glu 5cr Ala Thr Val' Phe 380 1152 ggc tgg cag ttt Gly Trp Gin Phe cgg ttt gtg ccg Arg Phe Val Pro cgc gaa acg gtg Arg Glu Tb: Val 1200 ggo gcg gtc eat aat gcc gcc tac cgg Gly Ala Val Ann Ann Ala Ala Tyr Arg eac ggt gtt tat gqc ggc Ann (fly Val Tyr Ala (fly 1248 tgg gcg cag gag T-rp Ale Gin Glu 420 gcg tct tat gcc Ala Sc: Tyr Ala 435 tgg cgg cag ttg ggc ggt ttg aac agt cgg gtt tcc Trp Arg Gin Laeu Gly Sly Lieu Ann 5cr Arg Val Set 425 430 1296 cgc cgc eec tat aag ggc gtt gog Arg Arg Asn Tyr Lys Sly Val Ala 440 gct ttc teg ace Ala Phc Sc: Thr 445 gct ttg agc cc Ala Lieu Ser His 1344 gag gcg Glu Ala 450 cad cgc aec cgc Gin Arg Asn Arg tgg aet gtc tcg Trp Ann Val Sc: 1392 aeg ttg tcg tac Lys Lcu 5cr Tyr ggt etc gtg ccc gcg ttg eat tat cgt Sly Ile Val Pro Ala Lieu Asn Tyr Arg ggc agg acg gaa Sly Arg Thr Glu aet gtg ccg tat gcg aaa cgc cgc aec Asn Val Pro Tyr Ala Lys Arg Arg An 490 agc gag Sc: Glu 495 1440 1488 1515 gtg ttt gtg tcg gcg gat tgg cgg ttt WO 01/85772 Val Phe Val Ser Ala Asp Trp Arg Phe 500 505 PCT/GB01/02003 <210> 118 <211> 505 <212> PRT <213> Neisseria meningitidis <400> 118 Met 1 Val Ala Asp Leu Asn Lys Leu Glu Ala 145 Phe Val Ser Leu Tyr Ser Ala Leu Leu Trp Lys Leu Ala Gly Asn Leu Pro Ala Lys 115 Leu His 130 Ala Ala Ala Glu Gly Arg Gly Gly Phe Ala Gin Val His Gin Asp 100 Leu Gly Glu Ala Phe 180 Ile Tyr Gly Gin Asn Lys 70 Asp Asp Gly Asn Asp 150 Lys Lys Pro Gly Phe Leu Val Val Trp Cys Ala Ala Gly Ala Asp Ala 25 Arg Ser Val Asp Ala Pro 55 Met Leu Glu Leu Ile Ala Ala Val Leu 105 Arg Pro Ala 120 Ala Ala Asp 135 Asp Phe Arg Leu Asp Leu Lys Ala Glu 185 Ala Val Asn Ala Ile Asp Lys Val Val Ser Leu Leu Ala Gly Arg Ala Val Arg Ile 140 Lys Ser 155 Ala Pro Leu Thr Asn Ala Asp Asp Ala Glu Gly Asp Asp Val Leu Tyr Arg Ala 110 Arg Tyr Leu Asp Glu Arg Leu Glu 175 Trp Arg 190 Ann Ala 205 WO 01/85772 Pro Gin Tyr cys Arg 210 Arg Ala Glu Arg Ala 225 Leu Thr Ala Leu Ala 245 Gly Gly Thr Ser Tyr 260 Phe Gly Arg Ala Tyr 275 Ala Gly Ile Leu Pro 290 Phe Asp Ala Lys Thr 305 Met Leu Ala His Gly 325 Asn Pro Gly Trp Gin 340 Tyr Arg Glu Gin Asp 355 Phe Tyr Val Ser Ser 370 Gly Gly Trp Gin Phe 385 Gly Ala Val Asn Asn 405 Trp Ala Gin Glu Trp 420 Ala Ser Tyr Ala Arg 435 Glu Ala Gin Arg Asn Asn Gly 215 Gly Leu Asn His Phe Ser Gly Trp 280 Tyr Gin 295 Arg Val Gly Val Ser Val Ala Glu 360 Lys Arg 375 Arg Phe Ala Tyr Gin Leu Asn Tyr 440 Glu Trp PCT/GB01/02003 Val Ser Glu Lys 240 Asn le 255 Asp Gly Gin Thr Asp Gly Pro Tyr 320 Arg Pro 335 Gin Arg Asp Gly Val Phe Val Gly 400 Ala Gly 415 Val Ser Ser Thr Ser His WOO01/85772 PCTGBOIO2003 Asp Lys Leu Ser Tyr Lys Gly Ile Val. Pro Ala Lea Asn Tyr Arg Phe 465 470 475 480 Gly Arg Thr Giu Sex Asn Val Pro Tyr Ala Lys Arg Arg Asn Set Giu 485 490 495 Val Plie Val Set Ala Asp Tip Arg Pbs 500 505 <210> 119 <211> 756 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <2.22> <400> 119 atg gea aeg cat Met Giu Thr His 1 get gtt ttg aaa Asp Veal Lou Lys tee gcg ttc ogc Tyr Ale Phe Arg age cat cat gcg Sex His His Ala att ttg eat tat Ile Leu Asn Tyr ttg ace gag ggc Leu Thr Giu Gly ggc tgt ttt Gly Cys Phe cag gce cac Gin Ala His att tog ott le Sex Leu cac aec gag His Asn Giu gtg gog cgg Val Ale Arg 70 ttg tcg cgg Leu Sex Krg ttg egg aag Lou Arg Lys geg gcg ate Ale Ale Ile ttg egg ceg Leu Arg Gin egg ttc gag Arg Ph. Giu atg ctg ttt Met Leu Phe ttg egg gee Leu Arg Ala ggc gtg ctg geg gee atg geg gcg gaa atg aat gtc ggc gee gge ctg Giy Val Leu Ale G).u Met Ale Ale (flu Met Asn Vel Gly Asp Gly Leu WO 01/85772 PCT/GB01/02003 tac ttg ggg gcg ggc gag ttg Tyr Leu Gly Ala Gly Glu Leu agc ggc ggc tte Set Gly Gly Phe egg cct tcg Arg Pro Set ata ctg gcg Ile Leu Ala 130 gac gcg atg Asp Ala Met gcg atg ttt gcg gcg gtc agc ttc gat Ala Met Phe Ala Ala Val Ser Phe Asp gat ttc aac acg gcg gaa aag gtg gtg Asp Phe Asn Thr Ala Glu Lys Val Val cat ttg ttt gca gaa His Leu Phe Ala Glu cgc gtc cgg cga gtc gat ttc caa aat cag gca aaa gac ggc Arg Val Arg Arg Val Asp Phe Gln ASn Gln Ala Lys Asp Gly aaa act Lys Thr 175 get ttg cag gag gcg ttg Ala Leu Gin cgt ate gaa Arg Ile Glu 195 Glu Ala Leu 180 cag gcg cgc Gln Ala Arg 185 cgt ttc gac ttg Arg Phe Ala Leu gag caa ate ggc cat gee aac gac Glu Gln Ile Gly His Ala Asn Asp 200 agt atg Set Met 205 cgt gee Arg Ala 220 cog aaa tac Pro Lys Tyr 190 ttt gtc att Phe Val Ile aaa ggg acg Lys Gly Thr te tgc Ser Cys 210 age cgc Ser Arg 225 gat ttg ggc gaa Asp Leu Gly Glu ggt ttc gtg tgc Gly Phe Val Cys aag gcg gcg Lys Ala Ala cag gaa gcg gcg Gln Glu Ala Ala gag get ttg aaa Glu Ala Leu Lys ctg gaa gag aag Leu Glu Glu Lys ccg ctg aag aag Pro Leu Lys Lys aaa aag aaa Lys Lys Lys 250 <210> 120 <211> 252 <212> PRT <213> Neisseria meningitidis <400> 120 Met Glu Thr His Set Gly Cys Phe Leu Arg Lys Thr Val Met Lys Asp WO 01/85772 Asp Val Leu Lys PCT/GB01/02003 Gin Gln Ala His Ala Ala Ile Gin Lys Lys Leu Gly 25 Tyr Ala Ser His Ile Leu Leu Thr Gly Val Tyr Leu Ile Leu 130 Ala Asp 145 Arg Val Ala Leu Arg Ile Set Cys 210 ser Arg 225 Leu Glu Phe Arg Asp His Ala Lys Asn Tyr Thr Glu Gly Glu Leu Ala Glu 100 Gly Ala Gly 115 Ala Asp Ala Phe Asn Thr Arg Arg Val 165 Gin Glu Ala 180 Glu Glu Gin 195 Asp Leu Gly Lys Ala Ala Glu Lys Leu 245 Arg Set Lys Glu Leu Ser Asp Glu 160 Thr Tyr Ile Thr Trp 240 <210> 121 WO 01/85772 <211> 570 <212> DNA <213> Noisseria maeningitidis PCT/GBOI/02003 <220> <221> ODS <222> <400> 121 atg gac aat cac gcc Met Asp Asn His Al1a 1 5 ata cgc cat aco cog Ile Arg His Thr Pro acg gtt too otg etc Tin Val ser Lou Ile tac ggc acg gat got Tyr Gly Tbr Asp Ala aac gga cat ccg ttc Ann (fly His Pro Phe cat ttc tta atc aaa His Phe Lau Ile Lys ggc gut atg gcg tao Gly Asp Met Ala Tyr 100 aca tgo aac gca ttt Lys Cys Asn Ala Pile 115 gaa ccc ttt acc gaa Giu Pro Phe Tin Giu 130 gca ctc tgc cgc cgc gaa gca cac tgg oaa aac ggc tgg ott caa ago Glu Ala His Trp Gin Asn Gly Trp Lau Gin 10 aat Asn cac His aag Lys Otg Lou ggc Gly ggc Giy ggC Gly 120 tao Tyr ttc Pile dac An ctg Lau ata. Ile aaa Lys gta Val 105 etc Ile cgt Arg agc 5cr ett Ile ttt Pile cac His acg Thr tcc 8cr gaa Giu tcg 5cr agg Arg otg Lou aac An ttg Lou cag Gin ttt Pile gaa Gin gaa Giu 140 ccc gtt acc goa gta ace gga cat cag gac Ala Lou Cys Arg Arg Tyr Pro Val Thr Ala Val Thr Gly His Gin Asp 145 IS1I50;1 WO 01/85772 atc gcg ccc ggc cgc aaa acc gac Ile Ala Pro Gly Arg Lys Thr Asp 165 cgg ata ogg gag ana ggg ttt ccc Arg Ile Arg Giu Lys Gly Phe Pro 180 PCT/GB01/0200S cc ggo cac ttt tto gao tgg cgg 528 Pro Gly His Phe Phe Asp Trp Arg 170 175 gta gao aga sat gcc gtc 570 Val Asp Arg Asn Ala Val 185 190 <210> 122 <211> 190 <212> PRT <213> Neisseria meningitidis <400> 122 Met Asp Asn 1 Ile Arg His Thr Val Ser Tyr Gly Tht An Gly His His Phe Leu Sly Asp Met Lys Cys Asn 115 Glu Pro Phe 130 Ala Len Cys 145 Ile Ala Pro Glu Ala Ser Pro Val Leu Val Glu 55 Phe Ser 70 Arg Asp His Ala Ser Ile Ala Gin 135 Tyr Pro 150 Lys Thr Gin Ser Ile Phe His Thr Ser Glu Ser Ala Gly 170 Ann Sly Pro Arg Ser Leu Ala Asn Thr Leu Val Gin ser Phe Leu Glu Leu Gin 140 Val Thr 155 His Phe Leu Thr Pro Leu Val Val Gly 110 Cys Leu His Asp WO 01/85772 WO 0185772PCT/GBO1/02003 Arg Ile Arg Glu Lye Gly rho Pro Val Asp Arg Asn Ala Val 180 185 190 <210> 123 <211> 2274 <212> DNA <213> Noisseria meningitidis <220> <221> CDS <222> (2274) <400> 123 atg aca aco tta cat ttc ta ggc Etc ccg cgt gtc ggt gao ttc ago Met Thr Thr Lou His Phe 5cr Gly Phe Pro Arg Vol Gly Ala Pho Arg gaa ttg aaa Giu Lou Lys gca caa gaa aaa tac tgg cgc aao Ala Gin Glu Lys Tyr Trp Arg Lys gaa atc ago gag Giu Ile Ser Giu caa gaa ttg ctg got gtt gct. aoo gao ttg cgc gag ao aac tgg aaa Gin Giu Leu Lou Ala Vol Ala Lye Asp Lou Arg Gin Lys Asm Trp Lys cao cog His Gin goc got gcc aoc gcc gat tao gtt gcc gta ggc got ttc act Ala Ala Ala Asn Ala Asp Tyr Val Ala Val Giy Asp Pho Thr too goc coo otc Tyr Asp HIS Ile gac ctg caa gtc Asp Lou Gin Vai acc ggc gag ott Thr Gly Ala Ile gao cgc Etc gga Etc goc agc ca Ala Arg rho ely Pho Asp Sor Gin aec oto Asn Leu 90 tct ttg gao coo ttc tta 5cr Lou Giu Gin rho Pho coo ctg gcg cgc ggt ac sea gao coo Etc got etc gaa Gin Lou Ala Arg ely Asn Lys Asp Gin Phe Ala Ile Glu 100 105 tgg ttc gac ac aaec toc coc ta ttg gtg cct gee Etc Trp rho Asp Thr Aen Tyr His Tyr Lou Vol Pro Giu rho oatg aco Mot Thr 110 cac gao get His Mla Asp WO 01/85772 PCT/GBOI/02003 acc gas Tbr Glu 130 ttt aaa goO Phe Lys Ala sat gc aaa cac tao gtt cas cag ctg caa gas Asn Ala Lys His Tyr Val Gin Gin Leu Gin Giu 135 140 cas got ttg ggc Gin Ala Leu Gly aas gee aaa ccg ace gtt gtg ggt Lys Ala Lys Pro Thr Val Val Gly 155 cog ttg Pro Leu 160 gao cgt Asp Arg 175 act tto ctg tgg gtc ggt aaa gas aaa ggc goc gtt gas ttc Thr Phe Len Trp Val Gly Lys Glu Lys Gly Ala Val Glu Phe otg ago ctg Leu Ser Leu got ttg gtt Ala Len Val 195 cct aaa ctg ttg cot gtt tao gtt gaa Pro Lys Len Len Pro Val Tyr Val Glu 185 atc otg act Ile Len Thr 190 gag cot get Giu Pro Ala gas gcc ggt gao Glu Ala Gly Ala tgg att can ate Trp le Git -le ttg act Len Thr 210 gtt gao ctg cot aaa gas tgg gta gaa Val Asp Le Pro Lys Glu Trp Val Glu 215 tao aaa gao gtt Tyr Lys Asp Val gao act ttg ago Ala Thr Len Ser gta sgt gco aaa Val Ser Ala Lys ate ctg ctg Ile Len Len 235 age act tao Ser Thr Tyr 240 720 tto ggt tot Phe Gly Sex gao ggc ctg Asp Gly Len gtt Val gao gas cac goo Ala Glu His Ala 245 ato gao ttg gts Ile Asp Len Val gca ttg ttg aaa Ala Len Len Lys 250 tee ctg cot gtt Sex Le Pro Val 255 cgc get Arg Ala 265 cct gas caa Pro Gin Gin otg gat gcg Leu Asp Ala 270 tto gc gao tao gac aaa gtt ctg Phe Ala Asp Tyr Asp Lys Val Len 275 280 tot gco ggo gtc Ser Ala Gly Val aaa gtt ttg gaa Lys Val Len Glu 300 att gao ggo cgo Ile Asp fly Arg 285 act gtt gag ott Thr Val Glu Len sat stt tgg ago Asn Ile Trp Arg 290 gc aac ctg sac Ala Asn Len Asn 295 Oaa goc aaa ctg ggc gao Gin Als Lys Lou Gly Asp 310 ogt ttg tgg att too Arg Len Trp Ile Ser 315 ago tot tgo Ser Ser Cys WO 01/85772 at; at; cac Leu Lou His aaa aa ccc Aen Lye Pro cao gaa ttg Gin Giu Lou 355 PCT/GBOI/02003 act ccg Thr Pro 325 ttt gac ttg Phe Asp Leu too gtt 5cr Val 330 gao gaa Aaa at; Giu Giu Lys Lou aaa gcc Lye Ala 335 1008 1056 gac ctg tac tct tgg ttg ga ttc acc atg Asp Lou Tyr Set Trp Leu Mla Phe flit Lou cgc gtc at; aaa Ar; Vol Leu Lye ga ttg aoc gao Ala Lou Asn Giu can aoa oa Gin Lye Tha 350 cgt got tat Ar; Asp tee cgt gcc Set Arg Ala 1104 gtt gac Vol Ala 370 goa gaa at; gat gee Giu Giu Lou Ala Ala 375 oga caa gct gct 5cr Gin Mla Ala gec gac Ala Asp 380 1152 aga aga gaa Set Set Giu ote coc cgt Ile His Ar; 390 gaa gac gtt gac aaa aga at; gao Ala Asp Vol Ala Lys Arg Lou Ala 395 1200 ttg cat gcc aac gca gac con cgca tee tcaca ttt gac gay Lou Pro Mla Asn Ala Asp Gi-n Ar; Lye 5cr Pro Phe Ala Asp agE ata Arg le 415 1248 ooo gag vaa Lye Ala Gin ate ggt tot Ilo Gly 8cr 435 gaa tgg ttg a at; Ala Trp Lou Aen Lou cat at; at; ccg Pro Lou Lou Pro aag acc aaa Thr Tin An 430 Cga ga gca Ar; Ala Ala 1296 tta ccg aaa act aco gaa ata aga Phe Pro Gin Thr Tin Giu Ile Arg 440 aaa ga Gin Ala 445 1344 ta aoo Pho Lys 450 ooa ggc gaa at; Eat gac gac gat toc Lys Gly Giu Leu 5cr Ala Ala Asp Tyr 455 gac gag at; ann Ala Ala Met Lye 1392 ;oa ote gac ttg Giu Ile Ala Lou gtt goa gag aa Val Giu Glu Gin aaa ctg gac ttg Lys Lou Asp Lou 1440 1488 gta at; gta aa Val Lou Val His gao gac gag agt 0Th Ala Glu Ar; gay at; gtt gna toe Etc Asp Met Val Glu Tyr Phe 495 gga gaa ttg ttg age ggt ttt ga ta ace aaa tat gga Gly Giu Leu Lou Set Gly Phe Ala Phe Thr Gin Tyr Gly tgg gto aa Trp Vol Gin 510 1536 WO 01/85772 age tac ggt Ser Tyr Gly 515 PCT/GB01/02003 tct egc tge gtt Ser Arg Cys Val cca ccg att Pro Pro Ile atc ttc-ggt gac gta Ile Phe Gly Asp Val -525 1584 age cgt Ser Arg 530 cct gaa gee atg ace gta get tgg tot act tac gca caa age Pro Glu Ala Met Thr Val Ala Trp Set Thr Tyr Ala Gln Ser 1632 ace ana cge cog Thr Lys Arg Pro aaa ggt atg ttg ace ggc cct gta acc Lys Gly Met Leu Thr Gly Pro Val Thr 555 1680 ctg caa tgg tct Leu Gin Trp Ser gtc cgc aac gat Val Arg Asn Asp cct cgc tct Pro Arg Ser acc gtg tgt Thr Val Cys 575 1728 aaa caa ate gca ctg get ctg aac Lys Gln Ile Ala Leu Ala Leu Asn 580 gaa gta ttg gat ctg gaa aaa Glu Val Leu Asp Leu Glu Lys 590 1776 gca ggc ate Ala Gly Ile 595 aaa gtc ate caa atc gac gaa cct gcc Lys Val Ile Gln Ile Asp Glu Pro Ala 600 cgc gaa ggc Arg Glu Gly 1824 ctg cct Leu Pro 610 ttg aaa cgt gcc Leu Lys Arg Ala gat tgg Asp Trp 615 gat gcc tac ctg Asp Ala Tyr Leu aac tgg gcc gge Asn Trp Ala Gly tct ttc cgc ctg ser Phe Arg Leu tct ace ggt tgc Ser Thr Gly Cys gac age act caa Asp Ser Thr Gin 1872 1920 1968 cac ace cat atg His Thr His Met tac tct gag tte Tyr Sex Glu Phe gac ate ctg cct Asp Ile Leu Pro get gca atg gat gcg gac gtg ate ace ate gag act tea Ala Ala Met Asp Ala Asp Val Ile Thr Ile Glu Thr Ser cgt tcc gac Arg Ser Asp 670 aac gac ate Asn Asp Ile 2016 atg gaa etc Met Glu Leu 675 ttg ace gcg ttc Leu Thr Ala Phe gaa ttc aaa tac Glu Phe Lys Tyr 2064 ggc cog Gly Pro 690 ggg gtt tac gac Gly Val Tyr Asp cac age ccg cgc His Ser Pro Arg cog aca gaa gcc Pro Thr Glu Ala 2112 WO 01/85772 gaa gtg gag Glu Val Glu 705 cgt ctg tgg Arg Leu Trp PCT/GB01/02003 ttg cgc aaa gcc ate gag gtt gta ccg gtt gaa 2160 Leu Arg Lys Ala Ile Glu Val Val Pro Val Glu 710 715 720 ccg gac tgc ggc ctg aaa aca cgc ggc tgg eaa 2208 Pro Asp Cys Gly Leu Lys Thr Arg Gly Trp Lys 730 735 ctc caa gtg atg atg aac gta acc cac aaa ttg 2256 Leu Gln Val Met Met Asn Val Thr His Lys Leu 745 750 aaa 2274 Lys <210> 124 <211> 758 <212> PRT <213> Neisseria meningitidis <400> 124 Met Thr Thr 1 Glu Leu Lys Gln Glu Leu His Giln Ala Phe Tyr Asp Ala Arg Phe Gln Leu Ala Trp Phe Asp 115 Phe Ser Gln Glu Val Ala Asn Ala Leu Asp Asp Ser Asn Lys Tyr His Thr Glu Phe Lys Ala Asn Ala Lys His Tyr Val Gln Gln Leu Gln Glu WO 01/85772 130 Ala Gin Ala 145 Thr Phe Leu Leu Ser Leu Ala Leu Val 195 Leu Thr Val 210 Tyr Ala Thr 225 Phe Gly Ser Asp Gly Leu Phe Ala Asp 275 Asn Ile Trp 290 Leu Gin Ala 305 Leu Leu His Asn Lys Pro Gin Glu Leu 355 Val Ala Glu 370 PCT/GB01/02003 Lys Pro Lys Gly 170 Pro Val 185 Trp Ile Trp Val Ala Lys Ala Leu 250 Arg Ala 265 Ser Ala Lys Val Leu Trp Ser Val 330 Leu Ala 345 Ala Leu Gin Ala Asn Ser Ser Glu Ile His Arg Ala Asp Val Ala Lys Arg Leu Ala Asp WO 01/85772 385 Leu Pro Ala Asn Lys Ala Gin Gin 420 Ile Gly Ser Phe 435 Phe Lys Lys Gly 450 Lys Glu Ile Ala 465 Val Leu Val His Gly Glu Leu Leu 500 Set Tyr Gly Ser 515 Ser Arg Pro Glu 530 Leu Thr Lys Arg 545 Leu Gin Trp Ser Lys Gin Ile Ala 580 Ala Gly Ile Lys 595 Leu Pro Leu Lys 610 Glu Ser Phe Arg 625 His Thr His Met Gln Arg Lys Asn Leu 425 Thr Glu 440 Ala Ala Glu Glu Glu Arg Ala Phe 505 Lys Pro 520 Val Ala Gly Met Asn Asp Asn Asp 585 Ile Asp 600 Trp Asp Thr Gly 395 Ser Pro 410 Pro Leu Ile Arg Asp Tyr Gin Glu 475 Asn Asp 490 Thr Gin Pro Ile Trp Ser Leu Thr 555 Ile Pro 570 Glu Val Glu Pro Ala Tyr Cys Glu 635 Phe Leu Gin Glu 460 Lys Met Tyr Ile Thr 540 Gly Arg Leu Ala Leu 620 Asp PCT/GB01/02003 400 Asp Arg Ile 415 Thr Thr Asn 430 Arg Ala Ala Ala Met Lys Asp Leu Asp 480 Glu Tyr Phe 495 Trp Val Gin 510 Gly Asp Val Ala Gin Ser Val Thr Ile 560 Thr Val Cys 575 Leu Glu Lys 590 Arg Glu Gly Trp Ala Gly Thr Gin Ile 640 Glu Phe Asn Asp Ile Leu Pro Ala Ile WO 01/85772 PCT/GBO1/02003 Ala Ala Met Gin Gly Pro 690 Giu Val 705 Arg Len Glu Thr Arg Ala Asp Val Ala Phe Asp Ile 695 Leu Arg 710 Pro Asp Len Gin Lys <210> 125 <211> 1137 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1137) <400> 125 atg agc cgt tta tgg ttt ttt gcc Met Ser Arg Len Trp Phe Phe Ala 1 5 att tac cta ttg ccc ang gag aca Ile Tyr Lou Len Pro Lys Glu ThrI can ctg ctt gat cat gct gcc gaa Gin Len Leu Asp His Ala Ala Glu 40 aac gtc aac aac ctc gan can atg 252 att ata Ile Ile Ctc gta Leu Val ggc ctg Gly Leu atg gaa atc cgc ctt Ile Arg Leu tcc atg cgc Ser Met Arg ccc gcg ttc Pro Ala Phe gcc gcc gac WO 01/85772 Asn Val Asn An caa gtc sac gcg Gin Val Ann Ala tac gcg ggc gcg Tyr Ala Gly Ala gaa ttt ccg cac Glu Phe Pro His 100 ccc gac gtg tgC Pro Asp Val Cys 115 atg gac ggc tcg Met Asp Gly Ser 130 tac sac gtc sac Tyr Ann Val- An 145 ggc gta tcc gtt Gly Val- 5cr Val ggc gaa gcc ggc Gly Giu Ala Gly 180 cac gac caa atg His Asp Gin Met 195 gat acc ggc gtt Asp Thr Gly Val- 210 gcg tac ass ttc Ala Tyr Lys Pbe 225 Leu G2lu Ccc gtc Pro Val 70 ccg ttt Pro Phe atc ccc Ile Pro cas cgc Gin Arg ctg atg Leu Met gcc acc Ala Thr 150 gaa ggc Glu Gly 165 gas ga Giu Glu ctc acc Leu Thr gac gca Asp Ala ace cgt Thr Arg 230 PCT/GBO1/02003 Asp ass 240 Lys s0 gas 288 Giu tog 336 8cr atg 384 Met gsa 432 Glu tgc 480 Cys 160 act 528 Thr tcc 576 Ser aa 624 Lys ggc 672 Gl y gac 720 Asp 240 atg 768 cgc atc ass gas atc cac cas gczc ctg ccc sat acs cac atc gtg WO 01/85772 Arg Ile Lys Glu Ile His Gln Ala Leu Pro Asn 245 250 cac ggc tcc age tcc gtt ccg caa gaa tgg ctg His Gly Ser Ser Ser Val Pro Gln Glu Trp Leu 260 265 tac ggc ggc aat atc ggc gaa acc tac ggc gtg Tyr Gly Gly Asn Ile Gly Glu Thr Tyr Gly Val 275 280 gtc gaa ggc ate aaa cac ggc gtg cgt aaa gtc Val Glu Gly Ile Lys His Gly Val Arg Lys Val 290 295 ttg cgc ctt got too ace ggc gcg gta cgc cgo Leu Arg Leu Ala Ser Thr Gly Ala Val Arg Arg 305 310 315 ccg toc gac ttc gat ccg cgc aaa tat ttg agc Pro Ser Asp Phe Asp Pro Arg Lys Tyr Leu Ser 325 330 atg aag caa atc tgc ctc gac ogo tac etc gcg Met Lys Gln Ile Cys Leu Asp Arg Tyr Leu Ala 340 345 cag gca ggc aaa at aaas cog gtt tcc ttg gaa Gin Ala Gly Lys Ile Lys Pro Val Ser Leu Glu 355 360 tat gcc aag ggc gaa ttg aac caa atc gtc aaa Thr. His aaa gtc Lys Val ccg gtt Pro Val 285 aac ate Asn lie 300 tac ctt Tyr Leu aaa acc Lys Thr ttc ggt Phe Gly aaa atg Lys Met 365 PCT/GB01/02003 Met gaa 816 Glu ate 864 Ile gac 912 Asp aac 960 Asn 320 gcg 1008 Ala ggt 1056 Gly cgt 1104 Arg 1137 Tyr Ala LysGly Glu Leu Asn Gln Ile Val Lys 370 375 <210> 126 <211> 379 <212> PRT <213> Neisseria meningitidis <400> 126 Met Ser Arg Leu Trp Phe Phe Ala Ala Lys Asn Ile Ile Ile Arg Leu 1 5 10 Ile Tyr Leu Leu Pro Lys Glu Thr Gln Met Ala Leu Val Ser Met Arg 25 WO 01/85772 PCT/GB01/02003 Gln Leu Leu Asp His Ala Ala Glu Asn Ser Tyr Gly Leu Pro Ala Phe 40 Asn Val Asn Asn Leu Glu Gln Met Arg Ala Ile Met Glu Ala Ala Asp 55 Gin Val Asn Ala Pro Val Ile Val Gin Ala Ser Ala Gly Ala Arg Lys 70 75 Tyr Ala Gly Ala Pro Phe Leu Arg His Leu Ile Leu Ala Ala Val Glu 90 Glu Phe Pro His Ile Pro Val Val Met His Gin Asp His Gly Ala Ser 100 105 110 Pro Asp Val Cys Gin Arg Ser Ile Gin Leu Gly Phe Ser Set Val Met 115 120 .125 Met Asp Gly Ser Leu Met Glu Asp Gly Lys Thr Pro Set Ser Tyr Glu 130 135 140 Tyr Asn Val Asn Ala Thr Arg Thr Val Val Asn Phe Ser His Ala Cys 145 150 155 160 Gly Val Ser Val Glu Gly Glu Ile Gly Val Leu Gly Asn Leu Glu Thr 165 170 175 Gly Glu Ala Gly Glu Glu Asp Gly Val Gly Ala Val Gly Lys Leu Ser 180 185 190 His Asp Gin Met Leu Thr Ser Val Glu Asp Ala Val Arg Phe Val Lys 195 200 205 Asp Thr Gly Val Asp Ala Leu Ala Ile Ala Val Gly Thr Ser His Gly 210 215 220 Ala Tyr Lys Phe Thr Arg Pro Pro Thr Gly Asp Val Leu Arg Ile Asp 225 230 235 240 Arg Ile Lys Glu Ile His Gin Ala Leu Pro Asn Thr His Ile Val Met 245 250 255 His Gly Ser Ser Ser Val Pro Gin Glu Trp Leu Lys Val Ile Asn Glu 260 265 270 Tyr Gly Gly Asn Ile Gly Glu Thr Tyr Gly Val Pro Val Glu Glu Ile 275 280 285 WO 01/85772 Val Glu Gly 290 Leu Arg Leu 305 Pro Ser Asp Met Lys Gin Gin Ala Gly 355 Tyr Ala Lys 370 His Gly Val 295 Thr Gly Ala 310 Pro Arg Lys Leu Asp Arg Lys Pro Val 360 Leu Asn Gin 375 PCT/GB01/02003 Thr Asp Glu Asn 320 Glu Ala 335 Glu Gly Asn Arg <210> 127 <211> 444 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 127 gtg gat get gca gca acg ctc ctg aca tac Val Asp Ala Ala Ala Thr Leu Leu Thr Tyr 1 5 cg ca aaca aa aaa aaa att ggt aat ttt Arg Pro Asn Thr Lys Lys Ile Gly Asn Phe ttc gac acg cac agg gca tct gcc cga ttt Phe Asp Thr His Arg Ala Ser Ala Arg Phe gtt aaa aat atg aca got aaa gga caa atg Val Lys Asn Met Thr Ala Lys Gly Gin Met aac gcc ctg cgt aaa gag cat gtt ccg gtt Asn Ala Leu Arg Lys Glu His Val Pro Val 256 WO 01/85772 ggt ate aaa Gly Ile Lys etc ctg aga Leu Leu Arg tec ace atc Ser Thr Ile ttg Leu aac Asn 100 gta Val caa ggt cag gtt Gin Gly Gin Val act tec gtc ace Thr Ser Val Thr ccg gca cgc tcc Pro Ala Arg Ser 120 ccg act tcg acc Pro Thr Ser Thr 135 ttc gat Phe Asp gtt tac Val Tyr cta caa Leu Gln caa gtg Gin Val 140 caa tac Gin Tyr aaa cac Lys His 110 cat gaa His Glu 125 gaa acc Glu Thr PCT/GB01/02003 gtt 288 Val att 336 Ile aga 384 Arg cag 432 Gin ccc caa gcc gca Pro Gin Ala Ala 130 cag oct gcc gaa Gin Pro Ala Glu 145 <210> 128 <211> 148 <212> PRT <213> Neisseria <400> 128 Val Asp Ala Ala 1 Arg Pro Asn Thr Phe Asp Thr His Val Lys Asn Met Asn Ala Leu Arg Gly Ile Lys Leu Leu Leu Arg Asn meningitidis Ala Thr Leu Leu Thr Lys Lys Ile Gly Asn Arg Ala Set Ala Arg Thr Ala Lys Gly Gin 55 Lys Glu His Val Pro 70 Gin Gly Gin Val Glu Thr Ser Val Thr Gin 257 Lys Cys Sex Tyr His Asn Leu Gin Ser Ile Phe Asp Val Tyr WO 01/85772 PCT/GB0l/02003 100 105 110 er Thr Ile Val Pro Ala Arg Ser Val Asn Leu Gin His Giu Ken Arg 115 120 125 ro Gin Ala Ala Pro Thr Ser Thr Leu Val Gin Val Giu Thr Val Gin 130 135 140 in Pro Ala Giu <210> 129 <211> 1308 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1308) <400> 129 atg tac gct aaa as ggc ggt ttg gga ctg gtt aaa agc cgc cgt ttc Met Tyr Ala Lys Lays Gly Gly Leu Giy Leu Val Lays 3cr Arg Arg Phe 1 5 10 gca cct ctt ttc gct acg cag ttt ctc ggc gcg ttc aac gac aat gtg Ala Pro Leu Phe Ala Thr Gin Phe Leu Gly Ala Phe Asn Asp Asn Val 25 ttc aaa acc gcg ctg ttt gtg atg att ggg ttt tac ggt ttg ggg caa Phe Lays Thr Ala Leu Phe Val Met Ile Gly Phe Tyr Gly Leu Gly Gin 40 aac ggc ttc ctg cct gcc gga cag atg ttg aac ttg ggc gcg ttg ctg Ken Gly Phe Len Pro Ala Giy Gin Met Leu Asn Leu Gly Ala Leu Len 55 ttt att ttg ccg tat ttc ctg ttt tcc tcg ctg tcg ggg cag ttg ggt Phe Ie Leu Pro Tyr Phe Len Phe Ser Ser Leu Ser Gly Gin Leu Gly 70 75 aac aaa ttc gac aag gcc gtt ttg gcg cgt tgg gcc aag gtg ctg gan Asn Lays Phe Asp Lays Ala Vai Len Ala Arg Trp Ala Lays Val Leu Gin 90 atg atc att atg gcg gtg gcg gca tac ggg ttt tat atc cgg tct gc WO 01/85772 Met Ile Ie Cog ctg ctt Pro Leu Leu 115 ttc ggg ccg Phe Gly Pro 130 gag ttg atg Glu Leu Met 145 ate ctg tto Ile Leu Phe tat att gtc Tyr Ile Val ggc ago ctg Gly Ser Leu 195 att gag tgg Ile Giu Trp 210 gtg cgg cao Val Arg His 225 tgg ttt gtc Trp Phe Val atc cat ca TIle His Leu ttt tcc ae Phe 5cr Ile 275 Met Ala 100 ttg gog Leu Ala aaa Lys ggc Gly cag Gi1n 165 ate Ile atg Met att Ile 000 Pro gog Ala 245 ggC Gly att Ile Tyr Gly 105 tgc atg Cys Met ctg ccc Leu Pro att gaa Ile Glu act gog Thr Ala 170 ctg gta Leu Val 185 ccc gc Pro Ala aca aaa Thr Lys gao att Ala Ile acg caa Thr Gin 250 gtt ttc Val Phe 265 tog gte 8cr Val PCT/GBO1/02003 Ala otg 384 Leu aaa 432 Lys gao 480 Ala 160 cct 528 Pro gtc 576 Val caa 624 Gin aeg 672 Thr ttt 720 Phe 240 caa 768 Gin ctg 816 Lew ago 864 S8cr egg gee cgg ctg egg ttg got tgg gta ag gtt ggt gag ttg ggt ttg WO 01/85772 PCT/GB01/02003 Arg Glu Arg Leu Arg Leu Ala Trp Val Thr Val Gly Ala Leu Gly Leu 290 295 300 acg gtt tgc ggc ttg gtt ttg gtg tgg ctg acg cac gga cac cgt-ttt 960 Thr Val Cys Gly Leu Val Leu Val Trp Leu Thr His Gly His Arg Phe 305 310 315 320 gaa ggg ctg aac ggc att ttt tgg ttt tta tcg caa gga tgg gca tat 1008 Glu Gly Leu Asn Gly Ile Phe Trp Phe Leu Ser Gin Gly Trp Ala Tyr 325 330 335 ccc gtg atg gcg gtg atg acg ctg at cggc ttt ttc ggc gga ttt ttc 1056 Pro Val Met Ala Val Met Thr Leu Ile Gly Phe Phe Gly Gly Phe Phe 340 345 350 tcc gtt ccg ctc tat ace tgg ctg caa ace gcc agt age gag act ttc 1104 Ser Val Pro Leu Tyr Thr Trp Leu Gin Thr Ala Ser Ser Glu Thr Phe 355 360 365 cgc gcc cgc gcc gtt gcc gcc aac aat atc gtt aac ggt att ttt atg 1152 Arg Ala Arg Ala Val Ala Ala Asn Asn Ile Val Asn Gly Ile Phe Met 370 375 380 gtt tcc get gcc gtt ttg agc gag gtg ttg ctg ttt ttg ttt gac age 1200 Val Ser Ala Ala Val Leu Ser Ala Val Leu Leu Phe Leu Phe Asp Ser 385 390 395 400 att tcc ttg ttg tat ctg att gtc get ttg ggc aat att ccg ttg tcg 1248 Ile Ser Leu Leu Tyr Leu Ile Val Ala Leu Gly Asn Ile Pro Leu Ser 405 410 415 gta ttt ttg att aag cgc gaa agg egg ttt tta gge gog gcg gca atc 1296 Val Phe Leu Ile Lys Arg Glu Arg Arg Phe Leu Gly Ala Ala Ala Ile 420 425 430 agg aaa aaa cct 1308 Arg Lys Lys Pro 435 <210> 130 <211> 436 <212> PRT <213> Neisseria meningitidis <400> 130 Met Tyr Ala Lys Lys Gly Gly Leu Gly Leu Val Lys Ser Arg Arg Phe 1 5 10 260 WO 01/85772 PCT/GB01/02003 Ala Pro Leu Phe Ala Thr Gln Phe Leu Gly Ala Phe Asn Asp Asn Val 25 Phe Lys Thr Ala Leu Phe Val Met Ile Gly Phe Tyr Gly Leu Gly Gin 40 Asn Gly Phe Leu Pro Ala Gly Gin Met Leu Asn Leu Gly Ala Leu Leu 55 Phe Ile Leu Pro Tyr Phe Leu Phe Ser Ser Leu Ser Gly Gin Leu Gly 70 75 Asn Lys Phe Asp Lys Ala Val Leu Ala Arg Trp Ala Lys Val Leu Glu 90 Met Ile Ile Met Ala Val Ala Ala Tyr Gly Phe Tyr Ile Arg Ser Ala 100 105 110 Pro Leu Leu Leu Ala Cys Leu Phe Cys Met Gly Ala Gin Ser Thr Leu 115 120 125 Phe Gly Pro Leu Lys Tyr Ala Ile Leu Pro Asp Tyr Leu Asp Asp Lys 130 135 140 Glu Leu Met Met Gly Asn Ser Leu Ile Glu Ser Gly Thr Phe Val Ala 145 150 155 160 Ile Leu Phe Gly Gin Ile Leu Gly Thr Ala Val Ala Gly Val Pro Pro 165 170 175 Tyr Ile Val Gly Ile Leu Val Leu Leu Val Ala Val Gly Gly Thr Val 180 185 190 Gly Ser Leu Phe Met Pro Ser Val Pro Ala Lys Ala Ala Asp Thr Gin 195 200 205 Ile Glu Trp Asn Ile Val Arg Gly Thr Lys Ser Leu Leu Arg Glu Thr 210 215 220 Val Arg His Lys Pro Val Phe Thr Ala Ile Ile Gly Ile Ser Trp Phe 225 230 235 240 Trp Phe Val Gly Ala Val Tyr Thr Thr Gin Leu Pro Thr Phe Thr Gin 245 250 255 Ile His Leu Gly Gly Asn Asp Asn Val Phe Ann Leu Met Leu Ala Leu 260 265 270 WO 01/85772 Phe Ser Ile 275 Arg Glu Arg 290 Thr Val Cys 305 Glu Gly Leu Pro Val Met Ser Val Pro 355 Arg Ala Arg 370 Val Ser Ala 385 Ile Ser Leu Val Phe Leu Arg Lys Lys 435 Ala Ala Leu Ala 295 Val Leu 310 Ile Phe Met Thr Thr Trp Ala Ala 375 Leu Ser 390 Leu Ile Gly Ser 280 Trp Val Val Trp Trp Phe Leu Ile 345 Leu Gin 360 Asn Asn Ala Val Val Ala PCT/GB01/02003 Phe Ser Gly Leu Arg Phe 320 Ala Tyr 335 Phe Phe Thr Phe Phe Met Asp Ser 400 Leu Ser 415 Arg Glu Arg Arg 425 Phe Leu Gly Ala Ala Ala Ile 430 <210> 131 <211> 876 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 131 atg aat tac gca aaa gaa atc aat gcg tta aat aac agc ctt tcc gat 48 Met Asn Tyr Ala Lys Glu Ile Asn Ala Leu Asn Asn Ser Leu Ser Asp 262 WO 01/85772 WO 0185772PCT/GBO1/02003 ttg aaa gge gao ate aao gtt tog ttt gaa ttt ttt eca Leu Lys G2ly Asp Ile Asn Val Sot Phe Giu Phe Pho Pro ceg aaa aao Pro Lys Asn gag esa atg Glu Gin Met gaa aeg atg ctg tgg gat tee ate eac cgt etg caa ace Giu Thr Met Leu Trp Asp 5cr Ile His Arg Leu Ginx Thr ctg cat Lou His eec aag tte gta tee gta ace tao gge gca aac tcc gge gaa Pro Lys Phe Val 5cr Val Thr Tyr Gly Mla Asn Set Gly Giu egc gac ego acg eac ggc ate gte aaa ego ate aaa cag gaa ace ggo Arg Asp Arg Thr His Gly Ile Val Lys Arg Ile Lys Gin Glu Thr Gly ttg gaa gea gea cog eac ctg ace gge ate gao gca tcc ccc Lou Glu Ala Ala Pro His Lou Thr Gly Ile Asp Ala Set Pro gac gaa Asp Glu ttg ege caa Lou Arg Gin gte gee otg Val Ala Lou 115 gee aan gac tat Ala Lys Asp Tyr gac agc gge ate Asp 5cr Gly Ile oge ego att Arg Arg Ile 11 0 cgt ggc gac gag ccg eec ggt tat gag aaa aaa cog ttt Mrg Gly Asp Giu Pro Pro Gly Tyr Giu Lys Lys Pro Phe tac gc Tyr Ala gaa gac ttg Glu Asp Leu gtt aag Va2l Lys eta tta cge Lou Lou Arg tcc gte gee gao tto gao 5cr Val Mla Asp Phe Asp tot gtg geg gca tat 000 gaa gtg eat eec gaa gee aaa tee Ser Val Ala Ala Tyr Pro Giu Val His Pro Giu Ala Lys eaa gee gat ctg Gin Ala Asp Leu aat otg aag ego sa Asn Lou Lys Arg Lys 170 ate gat geg ggt Ile Asp Ala Gly gca aso Ala Asn 175 cac gte ate His Val Ile ona ttt tte Gin Phe Phe ttt gao gta Pho Asp Val 185 gas ego tao GIU A-rgTyr etg ego ttc Lou Arg Phe 190 ego gao ego tge gtg atg ttg ggt ate gat gtg gaa ate gte cot ggt Arg Asp AMg Cys Val Met Lou Gly Ile Asp Val Giu Ile Val Pro Sly WO 01/85772 195 tt ttg cct le Leu Pro 210 cc aac gtc hr Asn Val PCT/GB01/02003 ace aac Thr Asn ate cca Ile Pro 230 ggc acg Gly Thr 245 gtc ctg Val Leu cgc age Arg Set cgc aac Arg Asn tec cgc Ser Arg gag ctg Glu Leu 280 gcg caa gta Ala Gln Val gaa ggt ttg Glu Gly Leu 240 ate gcc ate Ile Ala Ile 255 tte cac ttc Phe His Phe 270 cat att tta His Ile Leu <210> 132 <211> 292 <212> PRT <213> Neisseria <400> 132 Met Aan Tyr Ala 1 Leu Lys Gly Asp Glu Gin Met Glu Leu His Pro Lys Arg Asp Arg Thr meningitidis Lys Glu Ile Asn Ala Ile Asn Val Ser Phe Thr Met Leu Trp Asp 40 Phe Val Ser Val Thr 55 His Gly Ile Val Lys Asn Ser Phe Pro His Arg Ala Asn Lys Gin WO 01/85772 Leu Glu Ala PCT/GB01/02003 Glu Ala Pro His Leu Thr Gly Ile Asp Ala Ser Pro Asp 90 Leu Arg Val Ala Tyr Ala 130 Ile Ser 145 Gin Ala His Val Arg Asp Ile Leu 210 Thr Asn 225 Asp Asp Asp Met Tyr Thr Gly Val 290 Ile Ala Lys Asp Tyr 100 Arg Gly Asp Glu Pro 120 Asp Leu Val Lys Leu 135 Ala Ala Tyr Pro Glu 150 Leu Ile Asn Leu Lys 165 Thr Gin Phe Phe Phe 180 Cys Val Met Leu Gly 200 Val Thr Asn Phe Lys 215 Lys Ile Pro Ser Trp 230 Gln Gly Thr Arg Asn 245 Lys Val Leu Ser Arg 260 Asn Arg Ser Glu Leu 280 Pro Gly Ile Glu Lys 125 Val Ala 140 Glu Ala Asp Ala Arg Tyr Glu Ile 205 Lys Met 220 Met Tyr Ala Ser Lys Asp Thr Tyr Ala Ile His Ile Leu <210> 133 <211> 1371 <212> DNA <213> Neisseria meningitidis WO 01/85772 <220> <221> CDS <222> (1)..(1371) PCT/GO1/02003 <400> 133 atg agt eat Met Ser Asn 1 gca ctg acc Ala Leu Thr gcc att ccc Ala Ile Pro acc ggc ace Thr Gly Thr etc aaa cgt Leu Lys Arg cgt atg ctc Arg Met Len caa aac gtg Gin Asn Val ttg ttc ggc Len Phe Gly 115 ggc tgc gaa Gly Cys Glu 130 aaa cag aa& Lys Gin Lye 145 toc tct tta ggt ttg ggt aeg gaa ctc gtt tcc Ser ser Leu Gly Leu Gly Thr Glu Leu Val Set tao gaa Tyr Glu gc ggt Ala Gly gc gcc Ale Ala gcc ago Ala Set ccc acg Pro Thr ato aaa Ile Lye etg gao Met Asp 120 gcc ac Ala Thr 135 ttg aac Len Asn gaa gcc gac cgt etg ctg gat atg ggt ttt etc gac gac atc cgo aaa Gin Ala Asp Arg Met Len Asp Met Gly Phe Ile Asp Asp Ile Arg Lys WO 01/85772 PCT/GB01/02003 atc atg eag atg ctg coc cgc caa cgc caa acc ctg etc ttt tee gee Ile Met Gin Met Leu Pro Arg Gin Arg Gin Thr Leu Leu Phe Ser Ala ace tte tcc Thr Phe Ser 195 gee ceg ata cge Ala Pro Ile Arg etg geg caa gao Leu Ala Gin Asp ecc gaa ace gtc gat gte gec gcg eaa gac ace Pro Glu Thr Vai Giu Val Ala Ala Gin Asp Thr ttc atg aac gcg Phe Met Aen Ala 205 aac gee aac gte Asn Ala Ann Val cgc aae ctg etc Ag Ann Leu Leu 672 eag cac ate ate Gin His Ile Ile gte get ace att Val Asp Thr Ile cag eag Gin Lye 235 gaa egg etg att Gin Arg Lou Ile get ttg cat atg Asp Lou His Met eag gtc ate gtg Gin Val Ile Val tte tge Phe Cys 255 aaa ace aaa eaa age gte gac cgc Lye Thr Lys Gin Her Val Asp Arg 260 acg ego gaa etg Thr Arg Gin Leu gtg cgo cge Val Arg Arg 270 eaa age egg Gin Ser Arg tac etg tee Ann Lou 5er 275 gca cag geg ata Ala Gin Ala Iie ggc gac cgt tee Gly Asp Mg Ser otc gaa Leu sin 290 aca ete aae gee tte aaa gae gge ate Thr Len Asn Ala Phe Lys Asp Sly An 295 ege gte etc gte Arg Val Leu Val ace gat ate gee Thr Asp Ile Ala cgg ggg ctg gat Arg Gly Lou Asp gee gat ctg ccc Ala Giu Len Pro gte ate aat tao Val Ile Asn Tyr atg ccc gee eag Met Pro Mla Gin gaa gac tac gte Glu Asp Tyr Val cac cgo His Arg 335 960 1008 1056 1104 ate ggg cgo Ile Gly Arg ggg cgc geg gge geg gao gge gtg gcg Gly Mrg Ala Sly Ala Asp Giy Val Ala att te ctg Ile Ser Leu 350 atg gao gaa tee Met Asp Giu Ser gaa cag aaa atg ttt Glu Gin Lye Met Phe gaa tee att aaa gag otg ace Glu Ser Ile Lye Giu Len Thr WO 01/85772 355 ;c aac aag tsr Aen Lys PCT/GBOI/02003 ctg ctc atc Leu Leu Ile 365 gag cog Glu Pro atg cgc Met Arg cgc gaa Arg Glu got tgc Ala Cys 430 ogg aeg Axg Thr 445 oaa tgg Gin' Trp gaa con Glu Pro 400 aaa aac Lys Asn 415 gga aaa Gly Lys tge goc Cys Ala 1152 1200 1248 1296 1344 1371 <210> 134 <211> 457 <212> PRT <213> Neisseria <400> 134 Met Sex Asn Pro 1 Ala LOU Thr Ala Ala Ile Pro Lys Thr Gly Thr Gly Leu Lys Arg Tyr meningitidis PMe Ser Set Leu Gin Gly Tyr Glu Al1a Leu Ala Gly Lys Tin Ala Ala Ala Tin Ala 3cr WO 01/85772 Arg Met Leu Gin Asn Val Leu Phe Gly 115 Gly Cys Glu 130 Lys Gln Lys 145 Glu Ala Asp Ile Met Gin Thr Phe Ser 195 Pro Glu Thr 210 Glu Gln His 225 Glu Arg Leu Lys Thr Lys Asn Leu Ser 275 Leu Glu Thr I 290 Ala Thr Asp I 305 Val Ile Asn I Val Gin 100 Gly Ile Asn Arg Met 180 Ala Val Ile Iie Gln 260 Ala Leu Ile Tyr SLeu Gly Met Val Ile Met 165 Leu Pro Glu Ile Val 245 Ser Gln Aan Ala Glu 325 Thr Pro Thr Tyr Ile Lys Asn Met Asp 120 Val Ala Thr 135 His Leu Asn 150 Leu Asp Met Pro Arg Gin Ile Arg Lys 200 Val Ala Ala 215 Ala Val Asp 230 Asp Leu His Val Asp Arg Ala Ile His 280 Ala Phe Lys 295 Ala Arg Gly 310 Met Pro Ala Arg Asn 105 Lys Val Lys Gly Arg 185 Leu Gin Thr Met Val 265 Gly Asp Leu Gin Glu 90 Leu Gin Gly Val Phe 170 Gin Ala Asp Ile Asn 250 Thr Asp Gly Asp Pro 330 Leu Pro Thr Arg Glu 155 Ile Thr Gin Thr Gin 235 Gin Arg Arg Asn lie 315 Glu Ala Asp Leu Arg Ala Asp 125 Leu Leu 140 Ile Val Asp Asp Leu Leu Asp Phe 205 Thr Asn 220 Lys Arg Val Ile Glu Leu Ser Gin 285 Leu Arg 300 Ala Glu Asp Tyr Gin His 110 Leu Asp Val Ile Phe 190 Met Ala Asn Val Val 270 Gin Val Leu Val PCT/GB01/02003 Ile Asp Thr Val Arg Ala His Val Leu Asp 160 Arg Lys 175 Ser Ala Asn Ala Asn Val Leu Leu 240 Phe Cys 255 Arg Arg Ser Arg Leu Val Pro Phe 320 His Arg 335 WO 01/85772 Ile Gly Arg Met Asp Glu 355 Gly Asn Lys 370 Trp Glu Gin 385 Arg Gin Arg Thr Arg Pro Ile Ala Gly 435 Leu Leu Gin 450 Gly Arg Glu Gin Leu Ile Gly Ala 390 Arg Tyr 405 Asn Ala Sex Arg Arg Tyr Gly Ala Asp 345 Met Phe Glu 360 Arg Ile Glu Pro Glu Lys Ser Ala Lys 410 Asn Asp Ala 425 Ser Arg Arg 440 Val Lys Gly Val Ala Set Ile Lys 365 Gly Phe Glu 380 Pro Glu Met 395 Ala Gin Arg Gly Ala Ala Glu His Arg 445 PCT/GB01/02003 Leu Thr Trp Pro 400 Asn Lys Ala <210> 135 <211> 1374 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1374) <400> 135 atg aaa cac ate cac att ate ggt Met Lys His Ile His Ile Ile Gly 1 5 att gcc gcc att gcc aaa gaa gca Ile Ala Ala Ile Ala Lys Glu Ala gcg aag atg tat ccg ccg atg age Ala Lys Met Tyr Pro Pro Met Ser 40 270 ate ggc ggc acg ttt atg ggt ggg Ile Gly Gly Thr Phe Met Gly Gly 10 ggg ttt gaa gtc age ggt tgc gat Gly Phe Glu Val Ser Gly Cys Asp 25 ace cag etc gaa gcc ttg ggc ata Thr Gin Leu Glu Ala Leu Gly Ile WO 01/85772 ggc gtg tat gaa ggc tto gao acc gcg cag ttg gao gaa ttt aaa PCT/GB01102003 gcc 192 Sly Val Tyr Glu Sly Phe Asp Thr Ala Gin Lou Asp (flu rho Lys Ala gao gtt Asp Val gaa gcg Giu Ala ctg got Lou Ala acg cac Tin His tat gc Tyr Ala 130 tto agc rho 5cr 145 agc oaa 8cr Gin ttt ttc rho Phe gtg ttg Val Lou Sly Ala 210 gaa ggo Giu Gly 225 atc ggo aat gto Ilo Gly Asn Val 70 aac cgt ggg otg Asn Arg Gly Lou gtg ctg cac cat Val Lou His His acg aco aco gcg Thr Thr Thr Ala 120 gca ccg ggo tto Ala Pro sly Pho 135 goc ogC ctg ocg Ala Arg Leu Pro 150 ttt tto gto att Pho rho Val Ile 165 ogo tco aaa ttc Arg Sot Lys Pho ctg gaa tto gao Lou Giu Pho Asp 200 acc cag ttc cac Thr Gin Pho His 215 gtc tgo aac gga Val Cys Asn Sly 230 gcc sag ogo Ala Lys Arg 75 ct tat att Pro Tyr Ile 90 cat tgg gta His Trp Val 105 tot atg cto 5cr Mot Lou Ott ato ggc Leu Ile Sly caa aog ccg Gin Thr Pro 155 gaa goo gao Giu Ala Asp 170 gtg cat tac Val His Tyr 185 oao goc gac His Ala Asp cac oto gtg His Lou Val ogg cag oaa Arg Gin Gin 235 ggg Giy tcc 5cr Cto Lou gog Ala ggc Sly 140 ago Arg gaa Siu ogt Arg ato Ile Cgt Arg 220 ago 5cr atg gat gtg Met Asp Vai ggc ccg oa Sly Pro Gin ggo gtg gcg Sly Val Ala 110 tgg gtt ttg Trp Vai Lou 125 gta ccg ga Val Pro GlU osa gac cog Gin Asp Pro tao gao aco Tyr Asp Thr 175 ccg cgt aco Pro Mrg Thr 190 tto goc gat Pho Ala Asp 205 acc gtg cog Thr Vai Pro Otg caa gao Lou Gin Asp gtt Val tgg Trp ggg Sly gaa Giu aac Ass aac Asn 160 gcg Ala gac Ala ttg Lou tot S or act Thr 240 240 288 336 384 432 480 528 576 624 672 720 WO 01/85772 PCT/GBO1/02003 ttg gao aaa ggo tgo tgg acg cog gtg Leu Asp Lys Gly Cys Trp Thr Pro Va1 245 aaa ttc ggc acg gaa cac Lys Phe Gly Thr Giu His 255 ggc tgg cag Gly Trp Gin ctt gac ggo Leu Asp Gly 275 gc Ala 260 ggc gas gc aat Gly Glu Ala Asn get ggo tag ttc Asp Gly 3cr Phe gao gtg ttg Asp Val Leu 270 atg ggo gga Met Gly Gly aaa aaa gec gga Lye Lye Ala Gly gto got tgg sgt Val Ala Trp Ser cac aac His Asn 290 cgo atg sac gcg Arg Met Aen Ala otc gcc Leu Ala 295 gtc atc gco Val Ile Ala gcg ogt oat gco Ala Arg His Ala gto gao att cag Val Asp Ile Gin gao tgc gaa gc ttg ago aog ttt aaa Ala Cys Glu Ala Leu Ser Thx Phe Lys 315 960 gto aaa cgo cgo Val Lys Arg Arg gas ato aaa Glu Ile Lye ggo acg Gly Thr 330 gca aac ggt ato Ala Asn Gly Ile ace gtt Thr Val 335 1008 tao gac gao Tyr Asp Asp ggt ttg cgo Gly Lou Arg 355 ttc gco cac cat ccg Phe Ala His His Pro got ato gaa aco Ala Ile Gin Thr acg att oaa Thr Ile Gin 350 gtc cto gas Val Leu Glu 1056 1104 cag cgo gto ggc Gin Arg Val Gly gcg cgc atc ctc Ala Arg Ile Len Cog cgt Pro Arg 370 too sat acg atg Sex Asn Thr Met otg ggt aog atg Len Gly Thr Met goo ge ctg cc Ala Ala Leu Pro 1152 1200 ago etc asa gas Sex Len Lys Glu gac caa gtg tto Asp Gin Val Phe tao gc ggc ggc Tyr Ala Sly Gly gao tgg gao gtt gc gsa gc otc gcg Asp Trp Asp Val Ala Giu Ala Leu Ala 405 ttg ggc ggc agg leu Sly Gly Axg otg cac Len His 415 1248 gte ggo aaa Val Gly Lye tto gat goo tto gtt gc gaa ato gtg Phe Asp Ala Phe Val Ala Glu Ile Val 425 aaa aac gc Lys Aen Ala 430 1296 WO 01/85772 PCT/GB01/02003 gaa gca ggc gac cat att ttg gtg atg age aac ggc ggt ttc ggc gga 1344 Glu Ala Gly Asp His Ile Leu Val Met Ser Asn Gly Gly Phe Gly Gly 435 440 445 ata cac acc aaa ctg ctg gac got ttg aga 1374 Ile His Thr Lys Leu Leu Asp Ala Leu Arg 450 455 <210> 136 <211> 458 <212> PRT <213> Neisseria meningitidis <400> 136 Met Lys His Ile His Ile Ile Gly Ile Gly Gly Thr Phe Met Gly Gly 1 5 10 Ile Ala Ala Ile Ala Lys Glu Ala Gly Phe Glu Val Ser Gly Cys Asp 25 Ala Lys Met Tyr Pro Pro Met Ser Thr Gln Leu Glu Ala Leu Gly Ile 40 Gly Val Tyr Glu Gly Phe Asp Thr Ala Gin Leu Asp Glu Phe Lys Ala 55 Asp Val Tyr Val Ile Gly Asn Val Ala Lys Arg Gly Met Asp Val Val 70 75 Glu Ala Ile Leu Asn Arg Gly Leu Pro Tyr Ile Ser Gly Pro Gin Trp 90 Leu Ala Glu Asn Val Leu His His His Trp Val Leu Gly Val Ala Gly 100 105 110 Thr His Gly Lys Thr Thr Thr Ala Ser Met Leu Ala Trp Val Leu Glu 115 120 125 Tyr Ala Gly Leu Ala Pro Gly Phe Leu Ile Gly Gly Val Pro Glu Asn 130 135 140 Phe Ser Val Ser Ala Arg Leu Pro Gin Thr Pro Arg Gin Asp Pro Asn 145 150 155 160 Ser Gln Ser Pro Phe Phe Val Ile Glu Ala Asp Glu Tyr Asp Thr Ala 165 170 175 WO 01/85772 Phe Phe Asp PCT/GB01/02003 Lya Arg Ser Lys Phe Val His Tyr Arg Pro Arg Thr Ala 180 185 190 Val Gly Glu 225 Leu Gly Leu His Gly 305 Val Tyr Gly Pro Ala 385 Asp Leu Asn 195 Ala Ile 210 Gly Leu Asp Lys Trp Gin Asp Gly 275 Asn Arg 290 Val Asp Lys Arg Asp Asp Leu Arg 355 Arg Ser 370 Ser Leu Trp Asp Asn Gin Ile Gly Ala 260 Lys Met Ile Arg Phe 340 Gin Asn Lys Val Asp His 200 His His Gly Arg Pro Val Asn Ala 265 His Val 280 Ala Val Cys Glu Lys Gly Pro Thr 345 Gly Ala 360 Leu Gly Gin Val Leu Ala Phe Ala 205 Thr Val Leu Gln Gly Thr Phe Asp 270 Leu Met 285 Ala Arg Thr Phe Gly Ile Thr Thr 350 Ala Val 365 Ala Ala Ala Gly Gly Arg Val Gly Lys Asp Phe Asp Ala Phe Val Ala Glu Ile Val Lys Asn Ala WO 01/85772 WO 0185772PCTIGBOI/02003 Giu Mla Sl1y. Asp His Ile Leu Val Met Ser Asn 435 440 Ile His Thr Lys Leu Leu Asp Mla Leu Arg 450 455 <210> 137 <211> 876 <212> DNA <213> Neisseria meniagitidis Sly Sly.Phe Gly Gly 445 <220> <221> <222> <400> 137 ttg aco gtc cga acg ata ftag acg gog cat tat acc cta ttc cat tcc Leu Thr Val Arg Thr Lys Lys Thr Mla His Tyr Thr Leu Phe His gac oga at ccg Asp Arg Lys Pro tao atg act act ctc aaa ccc gcc ctg ccc got tat Ann Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr ctg gac aac Lcu Asp An atc cgo ato atc ctc tog cgc too agc cat ccc gcc aac Ile Arg Ie Ie Leu Thr Arg Tin 5cr His Pro Ala An atc ggc Ile Gly tot gc gcg cgc 5cr Ala Ala Arg atg ass act atg ggt ctg cac aaa ctg Met Lys Tin Met Sly Leu His Lys Leu aoo ato gtc gcc cot aat ctg atg got tog cog atg acg gat tao Thr Ile Val Ala Pro Ann Leu Met Ala Thr Pro Met Thr Slu An cog Pro coo gtg ttt gac cog gag cat cot cat tog ttt aaa tta cog Pro Val Phe Asp Pro Git HIS Pro Gin 5cr Phe Lys Let Pro gat gaa Sit Sit agc tto atc ctc got tc Phe Ile Let Ala Ser 100 ggc gcg gca gac Sly Mla Mla Asp 105 gtt ttg gaa tat goc tOO Val Lou Sit Asn Ala Tin 110 att gcc gct tot Ile Ala Mla Ser ttg gao gat gcc ott goc gao too Leu Asp Sit Mla Let Ala Asp Thr acc atc goc tgc Ti Ile Ala Cys WO 01/85772 PCT/GB01/02003 gcc ctg Ala Leu 130 ace age agc cgc cgc gaa att act gcg Thr Ser Arg Arg Arg Glu Ile Thr Ala 135 ctg caa ate ccg Lou Gin Thr Pro cgc gat ttg gta tee Arg Asp Leu Val Ser 145 gaa tta Glu Leu 150 ctg cag ace gea aae ega gge gag Leu Gin Thr Ala Asn Arg Gly Glu 155 gtg gca ctg gtt Val Ala Leu Val ggc aae gag act ely Asn Giu Thr gge ttg age atc Gly Lou Sox I@ gaa gaa Glu Glu 175 528 gtc eaa gec Val Gin Ala aac cga ctg atg Asn Arg Lou Met etc gcc caa gee Lau Ala Gin Ala 200 ate aac ggc aat lie Asn Gly Asn ccc gac tat Pro Asp Tyr 190 tao gaa ate Tyr Glu Ile ttc teg etc aae Phe Ser Leu Asn 195 gtg tag gte gtg tgt Val Gin Val Val Cys 205 ttc age Phe Ser 210 eaa ace ggt tcg Gin Thr Gly Ser atg ace cat Met Thr His ott eaa Lou Gin 220 caa gao gao cae Gin Glu Asp His gcg acc cac gag Ala Thr His Glu atc aaa gge atg Ile Lys Gly Met gee cat atg gaa Ala His Met Glu gtg atg aae gao Val Met Asn Asp ggo ttt ttc aac Gly Phe Phe Asn ege aae gge gag Arg Asn Gly Glu egt ctg Arg Lau 255 atg ege egt Met Arg Arg gao ate got Asp Ile Asp 275 cag age ctg tte Gin Ser Lau Pho cgc gct aac acg Arg Ala Asn Thr eaa ae gaa Gin Thr Glu 270 cat cgt ate His Arg Ile ate etg cgo ggt Ile Lou Arg Gly ttc aat ace gte Phe Aan Thr Vol cat aaa aaa gao His Lys Lys Asp 290 <210> 138 WO 01/85772 <211> 292 <212> PRT <213> Neisseria PCT/GB01/02003 meningitidis <400> 138 Leu Thr Vi 1 Asp Arg L] Leu Asp AP Ile Gly SE Thr Ile V Pro Val P1 Ser Phe 11 Ile Ala A3 13 Ala Leu Tt 130 Arg Asp Le 145 Val Ala Le Val Gln Al Phe Ser Le 19 Phe Ser GI 210 Ala Ala Th Arg Thr 5 Pro Asn Ile Arg Ala Ala Ala Pro Asp Pro Leu Ala 100 Ser Leu Ser Arg Val Ser Val Phe 165 Cys Asn 180 Asn Leu Thr Gly His Glu Ala His Tyr 10 Leu Lys Pro 25 Thr Arg Thr Lys Thr Met Ala Thr Pro Gin Ser Phe 90 Ala Asp.Val 105 Leu Ala Asp Ile Thr Ala Gin Thr Ala 155 Thr Phe Gly 170 Thr Ile Asn 185 Val Gin Val Thr His Leu Gly Met Val Phe His Pro Ala Pro Ala His Lys Glu Asn Pro Glu Asn Ala 110 Ile Ala Gin Thr Gly Glu Ile Glu 175 Pro Asp 190 Tyr Glu Glu Asp Met Glu WO 01/85772 PCT/GB01102003 225 230 235 240 Val Met Asn Asp Ile Gly Phe Pec An Ag AMg Asn Sly 0Th Arg Lcu 245 250 255 Met Arg Arg Met Gin 5cr Lieu Phe Gly Arg Ala Asn Thr Gin Thr Glh 260 265 270 Asp Ile Asp Ile lieu Arg Gly Phe Pet Asn Thr Val Ser His Arg Ile 275 280 285 His Lys Lys Asp 290 <210> 139 <211> 708 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (708) <400> 139 ttg cgg cag ctt gga tgt ttg att ggt Lieu Arg Gin lieu Gly Cys lieu Ile Gly 1 5 atg aat ttg aaa tta gtg ttt gaa tcg Met Asn Leu Lys lieu Val Phe Giu Ser gtg ttt gtg ttg atg ctg ttg atg agt. Val Phe Val lieu Met Leu Lcu Met Ber 40 gtc ttg cgc tgc atc aag ctg tat cgg Val lieu Arg Cys Ile Lys Leu Tyr Arg 55 gtc aaa cgg cat atg cgc gat act ttg Val Lys Arg His met Mrg Asp Thr Leu 70 aaa gtg cgc gcc gtc gat gcg cct ttg Lys Val Arg Ala Vai Asp Ala Pro Lieu 278 agt gtt 5cr Val ccc gtc Pro Vai acg tgg Thr Trp aaa. ggg Lys Gly aec gac Asn Asp ctg gcg Lieu Ala atc att Ile Ile etc ggt Ile Sly ttg gtt Lieu Val gcc gcc Ala Ala gtc gee Vel Glu gag gce Giu Ala WO 01/85772 PCT/GB01/02003 ttg cag tct tac egC aac tac cgc Leu Gln Ser Tyr Arg Ann Tyr Arg 100 aac gaa gcg toe Asn Glu Ala Set gaa ctg gcg Glu Leu Ala 110 cgo aac agt Arg Asn Ser cag get ttg Gln Ala Leu 115 ccg ttg aac gag Pro Leu Asn Glu ttg gtc att caa Leu Val Ile Gln atg gcg Met Ala 130 cag att atg cgc egg ttt gat tac ggg Gln Ile Met Arg Arg Phe Asp Tyr Gly 135 ace gcg ctt gec Thr Ala Leu Ala tec ate ggc gcg ace gcg ccg ttt ate ggg Ser Ile Gly Ala Thr Ala Pro Phe Ile Gly 145 150 ggg att tac cac gec ctg ate aat ate ggg Gly Ile Tyr His Ala Leu Ile Asn Ile Gly 165 170 ttc ggc acg gtt Phe Gly Thr Val caa age ggg cag Gln ser Gly Gin atg agt Met Ser 175 att gcg gcg Ile Ala Ala gcg ggt ttg Ala Gly Leu 195 gcc ggc ccg att Ala Gly Pro Ile gag gca ctg gtg Glu Ala Leu Val gcg acg gcg Ala Thr Ala 190 aac tte etc Asn Phe Leu ttc gtg gcg att Phe Val Ala Ile gcg gtg ttg gca Ala Val Leu Ala aat ego Asn Arg 210 gat ttg Asp Leu 225 ggc aca aaa ata ctg ace cag gat ttg Gly Thr Lys Ile Leu Thr Gln Asp Leu 215 gcg atg gcg cac Ala Met Ala His cac gtc cgo His Val Arg ctt aat caa aag Leu Asn Gln Lys gat age Asp Ser 235 <210> 140 <211> 236 <212> PRT <213> Neisseria meningitidis <400> 140 Leu Arg Gln Leu Gly Cys Leu Ile Gly Phe Phe Ser Val Gly Ile Ile WO 01/85772 Met Asn Leu Val Phe Val Lys Leu Leu Met Val Leu Val Lys Lys Val Leu Gin Gin Ala Met Ala 130 Ser Ile 145 Gly Ile Ile Ala Ala Gly Asn Arg 210 Asp Leu 225 lle Lys Met Arg 70 Val Asp Arg Asn Leu Asn Met Arg Thr Ala 150 Ala Leu 165 Ala Gly Val Ala Lys Ile Arg Leu 230 PCT/GB01/02003 Ile Gly Leu Val Ala Ala Val Glu Glu Ala Leu Ala Asn Ser Leu Ala Val Trp 160 Met Ser 175 Thr Ala Phe Leu Ala His <210> 141 <211> 812 <212> DNA <213> Neisseria meningitidis WO 01/85772 <220> <221> CDS <222> (810) <400> 141 PCT/GBO1/02003 atg act atg cac Met Thr Met His 1 gcc ctc ccc ogc tac goc gtt ttt ggc aac ccc gtc Ala Lou Pro Mrg Tyr Ala Val Phe Gly Asn Pro Val 5 10 C Ala ggc Gly gcg Ala gtt Val tot Ser aaa Lys aac Asn ttg Lou aaa Lys 145 aaa cac His gtt Val cag Gin s0 acc Thr gaa Giu aac Asn gac Asp ctt Lou 130 gaa Giu gcc agc 8cr gac Asp gcg Ala gta Val cgc Arg gga Gly atc Ile 115 ttg Lou cao His gag aaa Lys att Ile gtt Val ccg Pro gca Ala aaa Lys 100 acg Thr qgc Gly cgt Arg gaa caa Gin gaa Giu ttt Phe cag Gin gc Ala ggo Sly. aaa Lys ggc Gly 135 cgt Arg cog cat His 25 att le gaa Giu gcg Ala gog Ala aat Asn 105 att Ile gtg Vol gtc Val tto- gc Ala atc Ile tgc Cys gog Ala ctg Lou atc Ile ggc Gly 125 att Ile cgt Axg gc ctt Lou ggc Gly ggg Sly gac Asp att Ilo ggt Giy 110 000 Lys cct Pro acc Thr gto cag gaa Gin Glu ggt ttc Giy Phe gca aac Ala Asn gag cat Glu His ttt ctg Phe Lou ttg gcc Lou Ala acc atc Thr Ile gtt ttg Vol Lou cgc gcc AMg Ala 160 cog atg 96 144 192 240 288 336 384 432 480 528 Lys Ala Giu Glu Lou Ala Gin Lou Pho Sly Ile 5Th Ala Vol Pro Met WO 01/85772 PCT/GB01/02003 gcg gat gtg aac Ala Asp Val Asn 180 ggt cta aac ggt Gly Leu Asn Gly 195 tgc gcg ctt gcc Cys Ala Leu Ala 210 tta gat ttt gca Leu Asp Phe Ala 225 ggt atg cta gtc Gly Met Leu Val ttt acg ccc gat Phe Thr Pro Asp 260 <210> 142 <211> 270 <212> PRT <213> Neisseria <400> 142 Met Thr Met His 1 Ala His Ser Lys Gly Val Asp Ile Ala Gin Ala Val Val Thr Val Pro ggt ttt gat Gly Phe Asp cag att ccc Gin Ile Pro tac gat atg Tyr Asp Met 215 cga caa tcg Arg Gin Sex 230 ggt caa gcg Gly Gin Ala 245 atc cgc ccc Ile Arg Pro meningitidis Ala Leu Pro 2 Ser Pro Gin Glu Tyr Glu 2 Ser Thr Phe 55 Phe Lys Gin i 170 atc ate aac Ile Ile Asn ccg ccc gat Pro Pro Asp ggc tgc gcg Gly Cys Ala 220 aaa aaa act Lys Lys Thr 235 tec tac gcc Ser.Tyr Ala 250 gaa tac atg Glu Tyr Met Phe Gly Asn Phe Ala Leu Asp Ile Gly Gly Cys Gly Leu Ala Asp WO 01/85772 Ser Glu Arg Ala Lys Asn Gly Lys 100 Asn Asp Ile Thr 115 Leu Leu Leu Gly 130 Lys Glu His Arg 145 Lys Ala Glu Glu Ala Asp Val Asn 180 Gly Leu Asn Gly 195 Cys Ala Leu Ala 210 Leu Asp Phe Ala 225 Gly Met Leu Val Phe Thr Pro Asp 260 PCT/GB01/02003 Leu Leu Ala Ala Gly Ala Val Asn Thr Leu Ile Phe 90 Leu Arg Gin Val Ala Gly Pro Ala 150 Leu Ala 165 Gly Gly Gln Ile Tyr Asp Arg Gin 230 Gly Gin 245 Ile Arg Asn 105 le Val Val Phe Ile 185 Ile Tyr Ala Ala Ile 265 Gly Glu Val 140 Asn Glu Asn Asp Ala 220 Thr Ala Met Gly Leu 110 Lys Thr Pro Val Thr Arg Val Pro 175 Thr Ser 190 Phe Gin Lys Pro Asp Gly Trp Arg 255 Ala 270 <210> 143 <211> 1515 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1515) <400> 143 WO 01/85772 WO 0185772PCT/GBO1/02003 atg ttg tat ttc aga tao ggt ttt ttg gtt gtt tgg tgt gag goa ggt Met Leu Tyr Phe Arg Tyr Gly Phe Leu Val Val Trp Cys Ala Ala Gly 1 gtt tot Val Set gca ttg Ala Leu gat tgg Asp Trp ttg ttg Leu Leu sac ggc Asn Gly sag otg Lys Leu atg gag Leu Ala gas atg Glu Leu 130 gog 9g Ala Ala 145 ttt gc Phe Ala gtg ggg Val Gly geo tat Ala Tyr cag gtg Gin Val gtt gac Val Asp cat cog His Pro cag gcg Gln Al a gat tat Asp Tyr 100 ttg gag Lieu Ala ggg ga Gly Glu gag ttt GlU Phe gcg gas Ala Glu 165 ttt cgg Phe Arg 180 ggg gag Gly Ala cag ogg Gln Arg sat gat Asri Asp aas atg Lys Met 70 gat ttg Asp Leu gao gag Asp Ala gga sgg Gly Arg sat gag Asn Ala 135 gao gat Asp Asp 150 ass ttg Lys Lieu asa ass Lys Lys tag gat 5cr Asp aga gtg Arg Val oat agt His Sex tag ttg Set Lieu 90 tao ggo Tyr ely gag 9g Glu Ala gag ogg Glu Arg atg aag Lieu Lys 155 cog gag Pro Ala 170 ggg otg Gly Lieu ttg gat gao Lieu Asp Asp tgg gag ga Trp Ala Glu gao ggg gat Asp Gly Asp ago gaO gtg Arg Asp Val gat ttg tat Asp Lieu Tyr gag ogg got Ala Arg Ala 110 gag agg tat Ala Arg Tyr 125 ttg atg gat Lieu leu Asp ga gas sgg Ala Glu Arg gtt ttg gsa Val. Leu Glu 175 ggc tgg ogt Gly Trp Arg 190 WO 01/85772 WO 0185772PCT/GBO1/02003 tcg ggc gge ate agt cog geg gtc Sex Sly Sly Ie Ser Pro Ala Va]. 195 200 cg eag tat tge egg eaa aae gga Pro Gin Tyr Cys Arg Gin an Gly 210 215 egg gcg gag egg gcg gea gge ttg aat aga aat gee Ann Arg an Mla ant ant gee geg PAn Asn Ala Ala 205 tge agt gtc age Cys Ser Val. ggc egg eag Gly Arg Gin Ala Glu Arg Ala Ala Gly Leu aat tat Astn Tyr ate gag gcg gaa Ile Glu Ala Gin ctg acg geg ttg gea gat ant cat tat Leu Thr Ala Leu Ala Asp Asn His Tyr 245 ttg tte egt tee Leu Phe Arg Ser ant atc Ann Ile 255 gge gge aeg ely Gly Thr tte ggc aga Pbs Gly Arg 275 tat tat tte. agt Tyr Tyr Phe 5cr an tea get tat Lys Ser Ala Tyr gac gac ggg Ap Asp ely 270 egg tag aeg Arg Gin Tbr geg tat ttg ggt tgg tag tat aaa aat Ala Tyr Len Sly Trp Gin Tyr Ly's An 280O geg ggg Ala Gly 290 tt t gat Phe Ap 305 att ttg teg ttt Ile Len Pro Phe eag gtg eag ttg Gin Val Gin Len teg ggc agt Sex Gly Sex gac gge Asp Giy geg aa aen Ala Lys Thr aaa egg gta aae aat ege ege ctg ceg etg Lys Arg Val Ann Asn Arg Arg Len Pro Pro atg etg gtg ene Met Len Ala His gtc ggc gtg tag Val. Sly Vai Gin tee cat act tae Ser His Thr Tyr ege tea Arg Pro 335 1008 aae eeg gga tgg ena ttt tcg gte geg etg gaa eat tae Ann Pro Gly Trp Gin Phe 5cr Val Mla Leu Gin His Tyr ege eaa ege Axg Gin Arg 350 eag gac ggg Gin Asp Gly 1056 tat ege gaa tag gat agg geg Tyr Arg Gin Gin Asp Arg Ala 355 tac aat aac ggt Tyr Asn an Gly 1104 ttt tat Phe Tyt 370 gtt tog tog gea aaa ezgt ttg gge gaa Val. Sex Sex Ala Lys Arg Len ely Gin 375 yea act gtg tte Ala Thx Val Phe 1152 WO 01/95772 WO 0185772PCT/GBO1/02003 ggc tgg cag ttt Gly Trp Gin Phe egg ttt gtg cag ana agc gasacg gtg Arg Phe Val Pro Lys Arg Giu Thr Val 1200 ggc gog gtc Gly Ala Val aat aat gee goo tao Asn Asn Ala Ala Tyr ogg ego ac ggt gtt tat Arg Mrg Asn Gly Val Tyr goc ggo Ala Gly 415 1248 tgg gag cag gag tgg ogg cag ttg Trp Ala Gin Gin Trp, Arg Gin Len ggt ttg aaa agt Gly Len Asn Set ogg gtt tee Mrg Val 430 1296 gcg tat tat Ala Ser Tyr 435 gee ago ogo aao tat aag gge gtt gag got tto tog aca Ala Mrg Mrg Asn Tyr Lys Gly Val Ala Ala Phe Set Thr 1344 gag gog Gin Ala 450 caa ogo ac eo Gin Arg Asn Arg tgg aat gtc tog Trp Msn Val Ser etg got Len Ala 460 ttg ago cac Len 5er His 1392 aag ttg teg tac Lys Len Ser Tyr ggt ato gtg coo Gly Ile Val Pro ttg aat tat agt Len Asn Tyr Arg 1440 g agg acg gaa agt aat gtg cog tat Gly Mrg Thr Gin Sex Asn Val Pro Tyr aaa ogo ogo sac Lys Mrg rg Asn ago gag Set Gin 495 1488 gtg ttt gtg Val Phe Val gog gat tgg egg ttt Ala Asp Trp, Mg Phe 1515 <210> 144 <211> 505 <212> PRT <213> Neisseria <400> 144 Met Len Tyr Phe 1 Val Set Ala Ala Ala Len Len Gin meningitidis Mrg Tyr Gly Phe Len Val Val Trp Cys Ala Ala Giy Tyr Gly Ala Asp Ala Pro Ala Ile Len Asp Asp Lys Ala Gin Val Gin Arg 5cr Val Sex Asp Lys WO 01/85772 PCT/GB01/02003 Asp Trp Lys Val Asp Asn Asp Ala Pro Arg Val Val Asp Gly Asp Phe 55 Leu Leu Ala His Pro Lys Met Leu Glu His Ser Leu Arg Asp Val Leu 70 75 Asn Gly Asn Gln Ala Asp Leu Ile Ala Ser Leu Ala Asp Leu Tyr Ala 90 Lys Leu Pro Asp Tyr Asp Ala Val Leu Tyr Gly Arg Ala Arg Ala Leu 100 105 110 Leu Ala Lys Leu Ala Gly Arg Pro Ala Glu Ala Val Ala Arg Tyr Arg 115 120 125 Glu Leu His Gly Glu Asn Ala Ala Asp Glu Arg Ile Leu Leu Asp Leu 130 135 140 Ala Ala Ala Glu Phe Asp Asp Phe Arg Leu Lys Ser Ala Glu Arg His 145 150 155 160 Phe Ala Glu Ala Glu Lys Leu Asp Leu Pro Ala Pro Val Leu Glu Asn 165 170 175 Val Gly Arg Phe Arg Lys Lys Ala Glu Gly Leu Thr Gly Trp Arg Phe 180 185 190 Ser Gly Gly Ile Ser Pro Ala Val Asn Arg Asn Ala Asn Asn Ala Ala 195 200 205 Pro Gln Tyr Cys Arg Gln Asn Gly Gly Arg Gin Ile Cys Ser Val Ser 210 215 220 Arg Ala Glu Arg Ala Ala Gly Leu Asn Tyr Glu Ile Glu Ala Glu Lys 225 230 235 240 Leu Thr Ala Leu Ala Asp Asn His Tyr Leu Leu Phe Arg Ser Asn Ile 245 250 255 Gly Gly Thr Ser Tyr Tyr Phe Ser Lys Lys Ser Ala Tyr Asp Asp Gly 260 265 270 Phe Gly Arg Ala Tyr Leu Gly Trp Gin Tyr Lys Asn Ala Arg Gin Thr 275 280 285 Ala Gly Ile Leu Pro Phe Tyr Gin Val Gin Leu Ser Gly Ser Asp Gly 290 295 300 WO 01/85772 PCT/GB01/02003 Arg Val Asn Asn Arg Arg Leu Pro Pro Tyr 315 320 Phe 305 Met Asn Tyr Phe Gly 385 Gly Trp Ala Glu Asp 465 Gly Asp Ala Leu Ala Pro Gly Arg Glu 355 Tyr Val 370 Gly Trp Ala Val Ala Gin Ser Tyr 435 Ala Gin 450 Lys Leu Arg Thr Lys Thr Lys 310 His Gly Val 325 Trp Gin Phe 340 Gin Asp Arg Ser Ser Ala Gln Phe Val 390 Asn Asn Ala 405 Glu Trp Arg 420 Ala Arg Arg Arg. Asn Arg Set Tyr Lys 470 Glu Ser Asn 485 Tyr Arg 335 Arg Gin 350 Gin Asp Thr Val Thr Val Tyr Ala 415 Arg Val 430 Phe Ser Leu Ser Tyr Arg Asn Ser 495 Val Phe Val Ala Asp Trp Arg <210> 145 <211> 840 <212> DNA <213> Neisseria meningitidis <220> <221> CDS WO 01/85772 <222> (840). <400> 145 atg gat gan Met Asp Lys Glu 2 1 ctg ctg cat ttg Leu Lou His Leu ccc gtg ata gag I Pro Val Ile Glu ttt ggc ggn ggg Phe Sly Sly Sly I coo gan cog can Pro Glu Pro Gin 1 nag cag gtt ttg e Lys Pro Val Lou I cag cag cct nag g Gin Gin Pro Lys C 100 gan aaa ccg aaa c Glu Lys Pro Lys P 115 ccg gag gaa aaa c Pro Ala Glu Lys P 130 ccc ggc sat gat t Pro Sly A sn Ala S 145 gat sag gga acc g Asp Lys Sly Thr S 1 agc ggt aaa ggt a PCT/GB01/02003 aga Arg 5 ga %la tcn Ser gac ksp -cc Pro tag rye raa ;lu ca 1 ro ;cg ro ct er gt ly 65 go att Ile ntg et ggC Sly gga Sly gtg Val 70 cag Pro gag Glu gag Glu gtc Val goc Ala 150 atc Ile ggo tta Leu gtg Val aat Asn gca Ala 55 Ccc Pro gtg Val ocg Pro cct Pro gag GLu 135 aaa Lys aaa Lys ggt aca Thr gca Ala gtt Val 40 ccc Pro gag Glu gtt Val aaa Lys aag Lys 120 nag Lys gca Ala ggn Gly: gtc 289 ccc Prc ttg Leu 25 att Ile gaa Slu Cog Pro acg Thr Cct Pro 105 cog Pro cag Pro gac Asp gac Asp aaa Ala 10 atc Leu gaa ggt *ly cc Pro aaa Lys 90 gan Glu gaa Glu tot Ser ago Ser ggn Sly 170 ggc gtc gtg ttt Val Val Pho tgg Trp ttt Phe gca Ala aaa Lys 75 aag Lys gan Glu gcg Ala gaa Glu gag Glu 155 acg Thr gaa cag gcg Sln Ala gto gat Val Asp gga gcg Sly Ala act gtc Pro Val gag gat Ala Asp nag cog Lys Pro too gto Sex Val cac aag His Lys ttg ggo Leu Sly cat gc Pro Ala gag cog Glu Pro gag gat Ala Asp aaa ccc Lys Pro 110 gtc ccg Val Pro gc gan Ala GLu aat ggg Asn (fly gga gan Sly Gla C 175 gan gga gca Ala otg Leu gat Asp gcg Ala ccc Pro ntt Ile gaa Slu aaa Lys cat His gaa Glu 160 9gc fly gcc 48 96 144 192 240 288 336 384 432 480 528 576 WO 01/85772 Ser Gly Lys Gly 180 Ser Sly Sly Val Lys Sly 5Th His Gly Slu Sly PCT/GBOI/02003 Ala ggc Gly cog Pro gtt Val 225 ato Ile gag Ala gtc Val ago Ser 195 tat Tyr agc Set aaa Lys caa Gin gto Val 275 ggc ast Sly Asn acg ett Thz Leu tta Lau 200 atg Met cog Pro too Set caa Gin aat Asn 280 185 190 ago gcc ato ggc ago att cog cgc Arg Ala Asn Sly Se Ile Pro Arg 205 gag aat get gag cag ggt acg gtt Glu Ann Asp Glu Sin Sly Tb: Val 220 ggc ggt cat gtt gag too gtt aaa Sly Sly His Val Glu Ser Val Lays 235 240 ogg ttg gao eat gag gca ego aag Arg Leu Asp Ann Ala Ala Arg Lays 250 255 goc eat gcc tgg acg gag ttt aan Ala Ann Ala Tap Thr GlU Phe Lays 265 270 <210> 146 <211> 280 <212> PRT <213> Neisseria <400> 146 Met Asp Lays Glu 1 Leu Leu His Leu Pro Val Ile Glu Phe Sly Gly Sly Pro Giu Pro Sin meningititis Arg Ile Leu Tb: 5 Al1a Met Val Ala Se: Sly Asn Val Asp Siy Ala Pro Pro Val Pro Slu I 70 290 Ala Val Leu Trp G1w Phe Sly Ala Pro Lays WO 01/85772 Lys Pro Val PCT/GB01/02003 Leu Lys Pro Val Val Thr Lys Lys Ala Asp Ala Asp Ile 90 Gin Glu Pro Pro 145 Asp Ser Gly Pro Val 225 Ile Ala I Gln Lys Ala 130 Gly Lys Gly Ser Ala 210 Leu Val Ala Pro Lys 100 Pro Lys 115 Glu Lys Asn Ala Gly Thr Lys Gly 180 Ser Lys 195 Tyr Pro Ser Val Lys Ser Gin An Glu Glu Pro Lys Pro Pro Ser Gly 165 Ser Gly Thr Leu Ser 245 Gly Glu Val Ala 150 Ile Gly Asn Leu Val 230 Gly His Pro Glu 135 Lys Lys Gly Pro Ser 215 Ser Phe Phe Lys 120 Lys Ala Gly Val Leu 200 Met Pro Ser Pro 105 Pro Pro Asp Asp Lys 185 Arg Glu Gly Arg SGlu Glu Lys Glu Ala Lys Ser Glu Lys 140 Ser Glu Gin 155 Gly Thr Gly 170 Gly Glu His Ala Asn Gly Asn Asp Glu 220 Gly His Val 235 Leu Asp Asn 250 Pro Pro 125 Pro Gly Arg Gly Ser 205 Gln Slu A1a Lys 110 Val Ala Asn Gly Glu 190 Ile Gly Ser Ala Pro Pro Glu Gly Glu 175 Gly Pro Thr Val Arg 255 Glu Lys His Glu 160 Gly Ala Arg Val Lys 240 Lys Gin Ala Asn Ala Trp Thr Glu Phe Lys 265 270 260 Val Pro Val Lys 275 Phe Glu Leu <210> 147 <211> 1572 <212> DNA <213> Neisseria meningitidis <220> WO 01/85772 <221> CDS <222> (1)..(1572) PCT/GBOI/02003 <400> 147 atg Met I otg Leu ott Leu tgg Trp ogt Mrg tao Tyr ggo Gly tac Tyr aco Thr aca Thr 145 ggc Gly tto Phe gao Asp ttt Phe otg Leu cog Pro aog Thr otg Leu o-tt Leu Otg Leu 130 otg Leu gos Ala tat Tyr 5 tgg Trp goo Ala ttg Leu got Ala tto Phe otc Leu tat Tyr ggo Gly ttt Phe tao Tyr 165 ott cog aao aga Leu Pro Asn Arg cac His coo Pro ttc Ito tot Ser tgg Trp gc Ala goa Ala aaa Lys 135 Ogo Mrg caa Gin 000 gc Pro Ala ttt aco Pho Thr ggt goo (fly Ala too gc 5cr Ala ata oao Ile His gta cog Val Pro 105 otg tgt Lou Cys 120 ato ggt Ile Gly gaa ogt Gin Mrg atc cc Ile Thr oag Gin cto Len tto Phe tto Phe tac Tyr cc Thr ctg Lou tto Pho ttg Len tto Phe atg met tao Tyr goa Ala gto Val otg Leu goo Aila aco Thr tgg Trp gta Val otg Lou 155 cog gao ago cog oto gocr- ggo Pro Asp 5cr Pro Lou Ala Gly 170 WO 01185772 ttt goc cca Phe Ala Pro ctc ggt gtc Leu Gly Val 195 PCT/GB01/02003 ggc ggc atc cac atg gtt aca ctg gca Gly Gly le His Met Val Thr Len Ala 185 ace gee ttt Thr Ala Phe 190 tgg etg gtt ttg geg agt gat aac acc gca cgt tcg ggc Trp Leu Val Leu Ala Ser Asp Ass Thr Ala Arg Ser Gly aaa cgc ctg Lys Arg Leu 210 ett cca att ate Leu Pro Ile Ile ctg att gcc gcc Leu lie Ala Ala ett gcc gca gge Len Ala Ala Gly ace gec ega caa Thr Ala Arg Gin gac tte ace cgc Asp Phe Thr Arg gac ggc age cgc Asp Gly Ser Arg ace gtc gcc ctg ctt caa ggc sac atc Thr Val Ala Leu Len Gin Gly Asn Ile 245 caa ace ctc aaa Gin Thr Len Lys tgg cgt Trp Arg 255 gas gac eaa Glu Asp Gln ass acc act Lys Thr Thr 275 ate ceg acc ata Ile Pro Tir le aaa tat tac gas Lys Tyr Tyr Glu cag gte ggc Gin Val Gly 270 gee gac atc gtc Ala Asp lie Val ctg ccc gaa Leu Pro Glu ace gec atc ccc gte Thr Ala 285 Ile Pro Val atg ege Met Arg 290 caa aac ctg ceg Gin Asn Leu Pro aac ata ctg gca Asn Ile Leu Ala ttt gcc gas cag Phe Ala Giu Gin caa sac aac ggc Gin Asn Asn Gly geg etc gcc gte Ala Len Ala Val ate age caa tac Ile 8r Gin Tyr 912 960 1008 tcg gac ggc aae 8cr Asp Gly Asn tac gaa aae gcc Tyr Giu Asn Ala ate aac ctg acc Ile Asn Len Thr ggt tat Sly Tyr 335 cag gas aae sat eag gac ggc ate Gin flu Ass Asn Gin Asp Sly Ile 340 tac tac gcc Tyr Tyr Ala aaa aac cac Lys Asn His 350 1056 gte ccc tte gge Val Pro Phe Gly 355 gaa tae aaa Glu Tyr Lys ceg ctg ccc Pro Leu Pro 360 ttc ctg ace aeg cog ott Ph* Len Thr Thr Pro Leu 365 1104 WO 01/95772 WO 0185772PCT/GBOI/02003 tac aaa Tyr Lye 370 atg atg aat atg met met Ann met ctt tcc gac ttc Len 5cr Asp Phe aaa ggc ggc ggt Lye Gly Gly Gly aag caa tee gec etg Lye Gin Ser Ala Leu 385 tgt tac gas gac gga eye Tyr Gin Asp Gly 405 atg ass sac cas Met Lys Asn Gin atc gee ttc aac Ile Ala Phe Asn 1152 1200 1248 ttc ggc gac gas Phe Gly Asp Glu stt gee gee gee Ile Ala Ala Ala ass gat Lys Asp 415 gee sea ctg ctt gcc sat gee age sat atg gog tgg tac Ala Thr Len LCU Ala Asn Ala 5cr Ass Met Ala Trp Tyr ggs ass tee Gly Lys 430 1296 aac gee atg Asn Ala Met 435 tac cag cue etc Tyr Gin His Leu eta eas teg eag geg egg get atg gas Gin Gin 5cr Gin Ala Arg Ala met (flu 440 445 gee ace ase see gge ges ace gee ate Ala Thr Ann Thr Gly Ala Thr Ala Ile 460 1344 etc. gga Leu Gly 450 ege tat atg gte Arg Tyr Met Val 1392 atc tcc eec a ggc ate ate atc gee aa Ile Ser Pro Lys Gly Ass Ile Ile Ala Gin 465 470 ace gta ttg gsa ggs ese ate ass ggc tat Thr Val LeU Gin Gly His Ile Lye Gly Tyr 485 490 gee eaa eec gst Ala Gin Pro Asp 475 gte ggc gaa acg Val Gly Gin Thr acg Thr 1440 1488 ccc tat Pro- Tyr 495 stg saa acc Met Lyn Thr gca ctg att Ala Len Ile 515 agt tca tgg tgg 5cr Ser Trp T-rp ttg atg Len Met 505 ggc ata ttg Gly Ile Len ace eta gee Thr Leu Ala 510 1536 ett tte ate tte Len Pie Ile Phe sac ass gaa eac Aesn'Lye Gin His 15*72 <210> 148 <211> 524 <212> PRT <213> Meisseria meningitidis <400> 148 Met Phe Arg Arg Tyr Leu Pro Ann Arg Gin Met Asn Met Val Ser Lys WO 01/85772 PCT/GBO1/02003 Leu Leu Trp Arg Tyr Gly Tyr Thr Thr 145 Gly. Phe Leu 4 Lys Tyr 225 Thr Asp Phe Leu Pro Thr Leu Leu Leu 130 Leu Ala Ala krg 210 rhr lal Lys Ala Met Arg Thr Pro Ala 115 Pro Thr Ile Pro Val 195 Leu Ala Ala Tyr Ala Pro Phe Gin Asp 100 teu Arg Glu Gly Phe 180 Trp Leu Arg Leu Trp Ala Leu Ala Phe Leu Tyr Gly Phe Tyr 165 Gly Leu Pro Gin Leu 245 Gin Thr lie Val 70 Tyr Tyr Pro Ile Ala 150 Ser Gly Val Ile Thr I 230 Gln His Pro Phe 55 5er Trp Ala Ala Lys 135 Prg fln tIe ELeu Ile 215 ksp fly Pro Ala 25 Phe Thr 40 Gly Ala 5cr Ala Ile His Val Pro 105 Leu Cys 120 Ile Gly Giu Arg 1le Thr His Met 185 Ala Sex 200 Leu le I Phe Thr I Asn Ile I Leu Tyr Phe Ala Phe Val Tyr Len 75 Thr Ala 90 Leu Thr Phe Trp Len Vai Phe Leu 155 Pro Asp 170 Vai Thr Asp Asn fla Ala krg Pro 235 ksp Gin 250 Trp Pro Arg Phe Len Phe Len Len 140 Thr Ser Len Thr Leu 220 !\sp rhr Pro Tyr Leu Gly His Len Trp 125 Pro Gly Pro Ala Ala 205 Len Gly Len i Leu Tyr le Leu Asp Leu 110 Lys Ile Phe Leu Thr 190 Rrg Ala Ser Lys Len His Glu Thr Val Pro Lys Leu Gly Ala 175 Al a Sc: Ala Arg Trp Ile Phe Len Ala Ser Ala Phe Trp Trp 160 Gly Phe Gly Sly Ser 240 Dirg 255 Clu Asp Gin Val Ile Pro Thr Ile Gin Lys Tyr Tyr Glu Gin Val Gly 295 WO 01/85772 PCT/GB01/02003 Lys Met Ala 305 Ser Gln Val Tyr Lys 385 Cys Ala Asn Leu lle 465 Thr Met Thr Arg 290 Gin Asp Glu Pro Lys 370 Gin Tyr Thr Ala Gly 450 Ser Val Lys 260 Thr Ala 275 Gin Asn Asn Asn Gly Asn Asn Asn 340 Phe Gly 355 Met Met Ser Ala Glu Asp Leu Leu 420 Met Tyr 435 Arg Tyr Pro Lys Leu Glu Thr Gly 500 Asp Leu Gly Gly 325 Gin Glu Asn Leu Gly 405 Ala Gln Met Gly Gly 485 Ser Ile Pro Ser 310 Tyr Asp Tyr Met Leu 390 Phe Asn His Val Asn 470 His Ser Val Glu 295 Ala Glu Gly Lys Pro 375 Met Gly Ala Leu Arg 455 Ile Ile Trp Ile 280 Asn Leu Asn Ile Pro 360 Leu Lys Asp Ser Gin 440 Ala Ile Lys Trp 265 Leu Pro Ile Leu Ala Val Ala Val 330 Pro Tyr 345 Leu Pro Ser Asp Asn Gin Glu Leu 410 Asn Met 425 Gin Ser Thr Asn Ala Gin Gly Tyr 490 Leu Met 505 270 Glu Thr Ala Ile Pro Val 285 Ala Glu Gin Gin Tyr Thr 320 Thr Gly Tyr 335 Asn His Leu 350 Thr Pro Leu Gly Gly Gly Phe Asn Ile 400 Ala Lys Asp 415 Gly Lys Ser 430 Ala Met Glu Thr Ala Ile Asp Thr Glu 480 Thr Pro Tyr 495 Thr LeuAla 510 Ala Leu Ile Leu Phe Ile Phe Arg Asn 296 Lys Glu His WO 01/85772 PCT/GB01/02003 515 <210> 149 <211> 558 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 149 ttg gat ctc tac aaa ctc ggt Leu Asp Leu Tyr Lys Lou Gly tac aaa caa cat Tyr Lys Gin His acc cgt acc Thr Arg Thr gat gcg Asp Ala gaa ggc tat atc gag aaactg cac att acc ccc goc aat Giu Gly Tyr Ile Giu Lys Leu His Ile Thr Pro Ala Asn gcc cat gag Ala His Giu aaa cac ctg ccg ccg ttg ttg gaa gga ctg ccc aaa ggt acg acc Lys His Lou Pro Pro Lou Lou Giu Gly Lou Pro LyS Gly Thr Thr gtc tat Val Tyr gcc gac aaa Ala Asp Lys ggc tac gac agt gcg gas Gly Tyr Asp 5cr Ala Glu cgg cas cat ctg Arg Gin His Leu gas cat cag ttg ttg gac ggc att atg Glu His Gin Lou Lou Asp Gly Ile Het aaa gcc tgc cgc Lys Ala Cys Arg cgt ccg ctg tcg gaa acg caa acc aaa cge aac cgg tat ttg tcg sag Arg Pro Lou Ser Giu Thr Gin Thr Lys AMg Asn Arg Tyr Lou Ser Lys acc cgt tat Thr Arg Tyr gga tta sat tts aat cag gac aag gcg Gly leu Asn Len Asn Gin Asp Lys Ala 105 ata gta cgg caa ggc gag gca acg ccg lie Val Arg.Gin Gly Giu Ala Thr Pro 120 125 acg aag ccg Thr Lys Pro 110 tao tgg ttt Tyr Trp Phe cag aca gta cas Gin Tin Val Gin 115 aaa ttt aat cca cta tat gtg gtc gaa Lys Phe Asn Pro Lou Tyr Vai Val Glu cag ago ttc ggt acg ctg cac Gin 5cr Phe Gly Thr Len His WO 01/85772 130 cgt aaa ttc Arg Lys Phe 145 agt gcg caa Ser Ala Gin gcc aac aag Ala Asn Lys PCT/GB01/02003 cgc tat gcg Arg Tyr Ala 150 agc cat ctg Ser His Leu 165 cta agt gcg Leu Ser Ala 180 135 egg gca Arg Ala aag gcg Lys Ala ccc get Pro Ala 140 gcc tat tte gga ctg att aaa gtg Ala Tyr Phe Gly Leu Ile Lys Val 155 160 atg tgt ttg aac ctg ttg aaa gcc Met Cys Leu Asn Leu Leu Lys Ala 170 175 gec gcc Ala Ala 185 <210> 150 <211> 186 <212> PRT <213> Neisseria meningitidis <400> 150 Leu Asp Leu Tyr Lys Leu Gly 1 1 Glu Gly Tyr Ile Glu Lys Leu I Cys Lys His Leu Pro Pro Leu I Val Tyr Ala Asp Lys Gly Tyr Glu Glu His Gin Leu Leu Asp C Arg Pro Leu Ser Glu Thr Gln 1 Thr Arg Tyr Ser Gly Leu Asn I 100 Gin Thr Val Gin Ile Val Arg C 115 1 Lys Phe Asn Pro Leu Tyr Val V 130 135 Arg Thr Asn Ala Lys Gly Arg Gin Ala Cys Tyr Leu Ala Thr 110 Pro Tyr 125 Gly Thr WO 01/85772 PCT/GB01/02003 Arg Lys Phe Arg Tyr Ala Arg Ala Ala Tyr Phe Gly Leu Ile Lys Val 145 150 155 160 Ser Ala Gin Ser His Leu Lys Ala Met Cys Leu Asn Leu Leu Lys Ala 165 170 175 Ala Asn Lys Leu Ser Ala Pro Ala Ala Ala 180 185 <210> 151 <211> 1332 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1332) <400> 151 atg act gac ctg a, Met Thr Asp Leu A 1 aat cag gag ccg t' Asn Gin Glu Pro P] gat ccg ttt ttg gc Asp Pro Phe Leu kA gaa cgc atc gtc g; Glu Arg Ile Val G: cag gac gat aaa g< Gin Asp Asp Lys G. atg agt too gcc al Met Ser Ser Ala 1J ace gtc gat ttg g( Thr Val Asp Leu G] 100 acc ctg ttt gcc aac ctc aaa caa cgc aat ccc Thr Leu Phe Ala Asn Leu Lys Gin Arg Asn Pro 10 gcg gtt Ala Val 25 ccg aaa Pro Lys cgc gtc Arg Val caa gtc Gin Val tac aaa Tyr Lys aaa ttc Lys Phe 105 299 atg Met ago Ser gta Val cgc Arg ttc Phe caa Gin 110 WO 01/85772 asa sac goc ttg LYS An Mla Leu 115 gao tto gao ccc .Asp Phe Asp Pro 130 cas goc ttt atg Gin Ala Phe Met 145 gtt cog got ggo V&al Pro Mla Gly ttc gga cas tac Phe Gly Gin Tyr 180 ggc aaa ggt ttg Gly Lys Gly Leu 195 ggo tac ggo tgo Gly Tyr Gly Cys 210 gat agt ttt gas Asp 8cr Phe Glu 225 gog osa tac gcc Mla Gin Tyr Mla aco gtt too gac Thr Val 5cr Asp 260 ago gas gog oaa Set Glu Mla Gin 275 gaa cgg gtt got Glu Arg Val Ala PCT/GB01/02003 acc acc otg cot stg ggo ggo ggo ass ggo ggt too 384 Thr Thr Leu Pro Met Gly Gly Gly Lys Gly Gly as Lys cc Thr gao Asp 165 ass Lys ga Glu gto Val ggo Gly gc Ala 245 too 5cr oto Leu ac0 Thr ass Lys 135 oto Leu ggo Gly ato Ile 99o Gly tto Phe 215 ego Arg aaa Lys ggo Gly goo Ala goo Mla 295 gta Val 140 ggo Gly gas Glu tot Set ogt Arg otg Leu 220 ggt Gly ggo Gly 000 Pro ass Lys otg Leu atg met gog Ala ato Ile too Set coo Pro 205 oaa Gin too Ser gog' Ala gao Asp gas Giu 285 oas Gin ogo tto tgo Arg Phe qys gao soc gat Asp Thr Asp 160 ggo tao otg Gly Tyr Leu 175 gto otg soo Val Leu Thr 190 gsa gog cc Glu Ala Thr 500 ogo at Thr Arg Asn ggo aao gtg Gly Asn Val 240 asa gtg otg Lys Val Leu 255 ago ggt atg Sex Gly met 270 gto ogo cgo Val Arg Arg tso ttt gas Tvr Phe Gin 432 480 528 -576 624 672 720 768 816 864 912 WO 01/95772 WO 0185772PCT/GBO1/02003 sac oaa Asn Gin 305 at cog tgg Lys Pro Trp, gte gco gcc gas Val Ala Ala Glu goc otg Ccc tgo Ala Leu Pro Cys 960 1008 aoc cag sac gas Tin Gin Asn Glu ggo tgo tao gtc Gly Cyn Tyr Val gac gas gaa gc Asp Gin Glu Ala goc aaa soc ctg ttg gos sac Ala Lys Thr Leu Leu Ala Asn 330 335 aat atg cog tog act ttg ggo Asn Met Pro Sex Tin Lau Gly gtt goc gas Val Ala Giu ggt gog Gly Ala 345 1056 geg gte gag Ala Val Gin 355 eta ttt ato aaa Gin Phe Ile Lys ggo ato oto tao Gly Ile Leu Tyr cog ggo aaa Pro Gly Lys 1104 goc too Ala Sax 370 aae goc gge ggo Asn Ala Gly Gly gta gca Val Ala 375 act tea ggt Thr Ser Gly gaa atg age eat Gin Met Sex Gin gce ato cgc otg Ala Ile Arg Leu tgg act egt gaa Trp Thr Arg Gin gto gao caa ogo Val Asp Gin Arg 1152 1200 1248 ttc ggo sto atg Phe Gly Ile met ago ato cac ga Sex Ie His Gin tgt ctg ass tao Cyn Len Lys Tyr ggo aaa Giy Lys 415 gto ggc gao Val Gly Asp gta aao tao gto Val. Ann Tyr Val. ggt geg aac stt Gly Ala Ann Ile 9cC ggt tto Ala Gly Phe 430 1296 gto aaa gtt goc gat gog atg ctg gog caa ggc ttc Val Lys Val Ala Asp Ala Met Len Ala Gin Gly [The 1332 <210> 152 <211> 444 <212> PRT <213> Neisserit meningitidis <400> 152 Met Thr Asp Leu Asn Thx Len Phe Ala Ann Len Lys Gin Axg Ann Pro 1 5 10 Ann Gin Gin Pro Phe His Gin Ala Val. Gin Gin Val Phe Met 5cr Len WO 01/85772 PCT/GB01/02003 Asp Pro Phe Leu Ala Lys Asn P Glu Arg Gin Asp Met Ser Thr Val Lys Asn Asp Phe 130 Gin Ala 145 Val Pro Phe Gly Gly Lys Gly Tyr 210 Asp Ser 225 Ala Gin Thr Val Val Lys Ala Leu 100 Leu Pro Met Gly Tyr 180 Leu Cys Glu Ala Asp 260 Glu Pro Gly Gin Ile Gly Gly Val Thr Thr Lys Gly Thr Glu 150 Asp Ile 165 Lys Lys Glu Trp Val Tyr Gly Lys 230 Ala Glu 245 Ser Asn Glu A Val G Pro T Leu L Leu P 1 Lys S 135 Leu T Gly V Ile A Gly G 2 Phe A 215 Arg V Lys A Gly P ro Lys Tyr Lrg Val Val in Val Asn 'yr Lys Gly 90 ys Phe Leu 105 ro Met Gly er Asp Ala 'yr Arg His al Gly Gly 170 rg Asn Glu 185 ly Ser Leu 00 la Gin Ala al Leu Ile la Ile Gin 250 he Val Leu 265 Ser Leu Val Thr Arg Val Phe His Gin Val 110 Gly Gly Arg Phe Asp Thr Gly Tyr 175 Val Leu 190 Glu Ala Thr Arg Gly Asn Lys Val 255 Ser Gly 270 Ser Glu Ala Gin Leu Ala Ala Leu Ile Glu Leu Lys Glu Val Arg Arg WO 01/85772 275 Glu Arg Val 290 Asn Gin Lys 305 Thr Gln Asn Gly Cys Tyr Ala Val Glu 355 Ala Ser Asn 370 Asn Ala Ile 385 Phe Gly Ile Val Gly Asp PCT/GB01/02003 Ala Pro Glu Val 340 Gin Ala Arg Met Thr 420 Thr Trp Leu 325 Val Phe Gly Leu Gin 405 Val Tyr Gly 310 Asp Ala Ile Gly Ser 390 Ser Asn Ala 295 Val Glu Glu Lys Val 375 Trp Ile Tyr 280 Lys Ala Glu Gly Ala 360 Ala Thr His Val Glu Ala Ala Ala 345 Gly Thr Arg Glu Asn 425 Gin Glu Ala 330 Asn Ile Ser Glu Ser 410 Gly Gly Ile 315 Lys Met Leu Gly Glu 395 Cys Ala Leu 300 Ala Thr Pro Tyr Leu 380 Val Leu Asn 285 Gln Tyr Leu Pro Leu Leu Ser Thr 350 Ala Pro 365 Glu Met Asp Gin Lys Tyr Ile Ala 430 Val Lys Val Ala 435 Asp Ala Met Ala Gin Gly Phe <210> 153 <211> 867 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 153 atg aaa ccc ata cgg aaa gcc gtc ttc ccc gtc gca ggg atg gga aca 48 Met Lys Pro Ile Arg Lys Ala Val Phe Pro Val Ala Gly Met Gly Thr 1 5 10 WO 01/85772 ego ttc otg Arg Phe Leu gto gao aag Val Asp Lys ggc tgc aog Gly Cys Thr gaa gao oat. Glu ASP His ego oat aaa Arg His Lys aac att aco Asn Ile Thr cac gee gto His Ala Val 115 gtg att ttg Val Ile Lou 130 eaa atg gtc Gin Met Val 145 gaa act gto Giu Thr Val gaa cag etc Giu Gin Leu eag coo gaa Lys Pro Glu, 195 PCT/GBO1/02003 goc aco aag gca agc cog aaa gaa* atg ctg ccc ate 96 Ala Thr Lys Ala 5cr Pro Lys Glu Met Lou Pro Ile tao goc gta Tyr Ala Val 40 gtt aec gga Val Thr Gly tao gaa otc Tyr Giu Lou gaa cac gto Giu His Val cgt oag gog Arg Gin Ala 105 gc goo ate Ala Ala Ile 120 ate gat gee Ile Asp Ala ego ago ggo Arg 3cr Gly ace ggc toa Thr Gly 3cr 170 ogc att aoo Arg Ile Thr 185 aac ott goo Asn Leu Ala 200 gaa gas Giu Giu ego sac Arg Asn gaa aoo Giu Tin ogo aae Arg Asn gaa gca Giu Ala ggo gao Gly Asp as aa Gin Lys 140 sac agc Aen 5cr 155 tao gge Tyr Giy gge att Giy'lie gtc gtC Vai Val. gc Ala ass Lys gag Giu ate Ile ctg Lau gaa Gin 125 ggC Gly att Ile ate Ile gto Vai gga Giy 205 304 WO 01/85772 ctt ace ccg cgc Leu Thr Pro Arg 210 ggc aac gaa ate Gly Ann Glu Ile 225 gaa ttt gtc cta Glu Phe Val Leu ago aaa ctg ggc Ser Lys Leu Gly 260 ccc gaa acc ggc Pro Glu Thr Gly 275 gaa Glu <210> 154 <211> 289 <212> PRT <213> Neisseria <400> 154 Met Lys Pro Ile 1 Arg Phe Leu Pro Val Asp Lys Pro Gly Cys Thr Glu Glu Asp His Phe Arg His Lys Asp tta ctg acc Leu Leu Thr gga ctg ccg cge Gly Leu Pro Arg 220 gcc aag ctg ctc Ala Lys Leu Leu 235 acg cgc tac gac Thr Arg Tyr Asp gcc tac ggc ctg Ala Tyr Gly Leu 270 ttg gaa aaa tac Leu Glu Lys Tyr 285 PCT/GB01/02003 gcg 672 Ala cac 720 His 240 ggc 768 Gly cac 816 His acc 864 Thr meningitidis Arg Lys Ala Val Ala Thr Lys Ala Leu Ile Gin Tyr 40 Met Val Phe Val Asp Lys Ala Tyr Lys Leu Leu Glu Gly Met Met Leu Ala Val Lys Arg Glu Leu Ile Leu WO 01/85772 Asn Ile Thr PC] '/GBO1OZOOJ Cys Lou Tyr Ile Arg Gin Ala Glu Ala Leu Gly Leu Gpl3 100 105 110 His Val Gin Glu Gin Lys Leu Gly 225 Giu Set Arg Ala 120 Leu Ile 135 Glu Arg Gin Thr Gin Arg 5cr Asn 200 Asp Leu 215 Thr Asp Pro Phe Giu Ala Pro Giu Thr 275 Gin Gly Glu Pro Phe Arg Leu Lou Gin Tyr Arg Thr <210> 155 <211> 876 <212> DNA <213> Neisseria meningitidis <220> <221> CDS WO 01/85772 <222> (876) PCT/GBO1/02003 <400> 155 ttg Lou 1 gac Asp ctg Len ate Ile ae-o Tb: Ceo Pro agc Ser att Ile gee Ala ego Arg 145 gtg Val ace gte ega Tb: Val Arg seq aaa aag aeg gcg cat tat ace ota ttc cat tee Tb: Lys Lys Tb: Ala His Ty: Tb: Leu Phe His So: oga Arg gac Asp ggc Sly ate Ile gtg Val ttc Pbe gc Ala ctg Lou 130 gat Aep gca aa Lye aac Asn Lt Ser gto Val ttt Phe ate Ile get Ala 115 ace Tb: ttg Leu ctg Lou cg Pro ate Ile gee Ala gcc Ala gao Asp etc Leu 100 tot Se: age Se: gta VJal gtt Val aac Asn ego Axg gcg Ala czea Pro cog Pro get Ala ttg Lou ego Arg tee Se: ttc Phe atg met ate Ile oge Arg aat Asn 70 gag Gin too Ser gao Asp ego Arg gaa Gin 150 gge Giy act Tb: ate Ile gog Ala 55 ctg Lou cat His ggc Sly gaa Gin oge Arg 135 tts Leu sac Asn sot Tb: etc Len 40 atg met atg met oct Pro geg Ala gee Ala 120 gaa Gin etg Len gag Gin etc Lou 25 aog Thr aaa Lye gea Ala caa Gin gos Ala 105 ott Leu att Ile eag Gin act Tb: ass Lye ego Arg sa Tb: acg Tb: tog So: 90 gac Asp gee Ala act Tb: ace Tb: tte Pbe co0 gee Pro Ala aco age Tb: Se: atg ggt Met sly cog atg Pro Met 75 ttt aaa Pile Lye gtt ttg Val Lou gao ace Asp Tb: geg cog Ala Pro 140 gea. aac Ala Asn 155 gge ttg Sly Lou ctg Lou cat His ctg Leu aeg Tb: tta Lou gaa Giu ace Tb: 125 otg Leu oga Arg age Set ccc Pro coo Pro eac His gas Giu oog Pro sat Asn 110 ate Ile 055 Gin gge Sly ate Ile get tat Ala Tyr gee sac Ala Asn aaa otg Lye Leu sac cog Asn Pro gaa gas Glu Gin gee cc Ala Thr gee tgc Ala Cys ace cog Thr Pro gag aaa Giu Lys 160 gaa gas Gin Gin 96 144 192 240 288 336 384 432 480 528 165 gte osa gee tgc sac oga ct4 atg ace ato sac ggo sat cec gac tat WO 01/85772 Val Gln Ala Cys 180 ttc teg etc aac Phe Ser Leu Asn 195 ttc age caa ace Phe Ser Gln Thr 210 get gcg acc cac Ala Ala Thr His 225 gtg atg aac gac Val Met Asn Asp atg cge cgt atg Met Arg Arg Met 260 gac ate gat atc Asp Ile Asp Ile 275 cat aaa aaa gac His Lys Lys Asp 290 <210> 156 <211> 292 <212> PRT <213> Neisseria <400> 156 Leu Thr Val Arg 1 Asp Arg Lys Pro Leu Asp Asn Ile Ile Gly Ser Ala Asn Arg etc gee Leu Ala ggt tcg Gly Ser gag caa Glu Gin 230 ate ggc Ile Gly 245 cag age Gin Ser Leu Met caa gcc Gin Ala 200 ccc atg Pro Met 215 atc aaa Ile Lys ttt ttc Phe Phe ctg ttc Leu Phe Thr Ile Asn 185 gtg cag gtc Val Gin Val ace cat ctt Thr His Leu gge atg gtc Gly Met Val 235 aac cgc cgc Asn Arg Arg 250 gga cgc gcc Gly Arg Ala 265 PCT/GB01/02003 Tyr ate 624 Ile cac 672 His age 720 Set 240 ctg 768 Leu gaa 816 Glu ctg cge ggt ttt ttc aat ace gtc age cat cgt ate Leu Arg Gly Phe Phe Asn Thr Val Ser His Arg Ile meningitidis Thr Lys Lys Thr Ala His Tyr Thr Leu Phe His Ser 5 10 Asn Met Thr Thr Leu Lys Pro Ala Leu Pro Ala Tyr 25 Arg Ile Ile Leu Thr Arg Thr Ser His Pro Ala Asn 40 Ala Arg Ala Met Lys Thr Met Gly Leu His Lys Leu 308 WO 01/85772 Thr Ile Val Pro Val Phe Ser Phe lie Ile Ala Ala 115 Ala Leu Thr 130 Arg Asp Leu 145 Val Ala Leu Val Gln Ala Phe Ser Leu 195 Phe Ser Gin 210 Ala Ala Thr 225 Val Met Asn Met Arg Arg Asp Ile Asp 275 His Lys Lys I 290 PCT/GB01/02003 Ala Asp Leu 100 Ser Ser Val Val Cys 180 Asn Thr His Asp Met 260 Ile Asp Pro Pro Ala Leu Arg Ser Phe 165 Asn Leu Gly Glu Ile 245 Gin Leu Asn 70 Glu Ser Asp Arg Glu 150 Gly Arg Ala Ser Gln 230 Gly Set Arg Leu His Gly Glu Arg 135 Leu Asn Leu Gin Pro 215 Ile Phe Leu Gly Met Pro Ala Ala 120 Glu Leu Glu Met Ala 200 Met Lys Phe Phe Phe 280 Ala Thr Pro Met Thr Gin Ser Phe Lys Leu 90 Ala Asp Val Leu Glu 105 Leu Ala Asp Thr Thr 125 Ile Thr Ala Pro Leu 140 Gin Thr Ala Asn Arg 155 Thr Phe Gly Leu Ser 170 Thr Ile Asn Gly Asn 185 Val Gin Val Val Cys 205 Thr His Leu Gin Gin 220 Gly Met Val Ala His 235 Asn Arg Arg Asn Gly 250 Gly Arg Ala Asn Thr 265 Phe Asn Thr Val Ser 285 Asn Glu Ala Ala Thr Glu Glu 175 Asp Glu Asp Glu Arg 255 Thr Arg WO 01/85772 PCT/GB01/02003 <210> 157 <211> 1008 <212> DMA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1008) <400> 157 atg tcc ate aaa a, Met Ser Ile Lys I: 1 gtg atg ace cag c, Val Met Thr Gin P. tgc acg att gat gi Cys Thr Ile Asp VI cgt atg ccg gaa gi Arg Met Pro Glu Vi gcg ttg gag ttg ai Ala Leu Glu Leu L] cgc gga tac gat ca Arg Gly Tyr Asp GJ ate ate gcg ctg gc Ile Ile Ala Leu A] 100 gaa age cgt tat tt Glu Ser Arg Tyr Pt 115 cgt ctg cct ttg at Arg Leu Pro Leu Me 130 ctg att att tcc ccc agt tgg ata ggc gac tgc Leu Ile Ile Ser Pro Ser Trp Ile Gly Asp Cys 10 ttc cgc Phe Arg gca ccg Ala Pro 40 gag att Glu Ile 55 cgt tgg Arg Trp ate gtg Ile Val ggt ate Gly Ile ttg aac Leu Asn 120 gac aga Asp Arg 135 aag Lys tcg Ser aat Asn ggt Gly ggt Gly agg Arg cgc Arg get Ala gaa ctt Glu Leu atg gcg Met Ala cog ttc Pro Phe agg gat Arg Asp tct ttg Ser Leu acg ggt Thr Gly agg ctg Arg Leu 125 ctc gcg Leu Ala 140 cac His gtg Val gga Gly ttg Leu aaa Lys tat Tyr 110 gat Asp cat His cag gag gat ttt gac ggg cat tcg gga ttc ccc gag ttt tec att gat WO 01/85772 PCT/GB01/02003 Glu Asp Phe Asp Gly His Ser Gly Phe Pro Glu Phe Ser lie Asp gaa cgg cgg egg Glu Arg Arg Arg att tot gtc gaa Ile Ser Val Glu ttt ggc ttg gat Phe Gly Leu Asp att gga Ile Gly 175 aag cct gtt Lys Pro Val cgt tgg ccg Arg Trp Pro 195 get ttt tgt cog Ala Phe Cys Pro gcg gaa ttc ggg Ala Glu Phe Gly ccg gca aag Pro Ala Lys 190 tat tog gcg Tyr Ser Ala aca agg tat ttt gcc gag ttg ggc aaa Thr Arg Tyr Phe Ala Glu Leu Gly Lys 200 gcg ggt Ala Gly 210 tgg cag gtt tgg ctg ttc ggt tog caa Trp Gin Val Trp Leu Phe Gly Ser Gin 215 gat gat gaa att Asp Asp Glu Ile gag gaa atc aac Glu Glu Ile Asn ctt tea gac Leu Ser Asp ggt atg Gly Met 235 tgt gtc aat ttg Cys Val Asn Leu ggc aaa ace gat ttg tcg cag gca atg Gly Lys Thr Asp Leu Ser Gin Ala Met 245 acg gtc gtg tgc aac gac agc ggg ctg Thr Val Val Cys Asn Asp Ser Gly Leu 260 265 ttg ctg tcg ttg Leu Leu Ser Leu gcg gac Ala Asp 255 atg cac ttg gcg Met His Leu Ala gcg gca ttg Ala Ala Leu 270 acg cat acg Thr His Thr ggc agg aag Gly Arg Lys 275 gtg gtg gcg gtt Val Val Ala Val ggt tct tcc age Gly Ser Ser Ser ccg cct Pro Pro 290 ttg age gac agg gcg Leu Ser Asp Arg Ala 295 aaa ate gtc age ctg cac ttg gaa tgt Lys Ile Val Ser Leu His Leu Glu Cys 300 tcg ccc tgt ttt aag cgg gaa tgt Ser Pro Cys Phe Lys Arg Glu Cys ccg ctg ggg cat Pro Leu Gly His 315 acc gac tgc Thr Asp Cys aac agg ctg tat Asn Arg Leu Tyr ccc gag aag Pro Glu Lys 325 att gtg cag gcg gtt Ile Val Gin Ala Val 330 gaa gag gcg gta Glu Glu Ala Val 335 1008 WO 01/85772 <210> 158 <211> 336 <212> PRT <213> Neisseria <400> 158 Met Ser Ile Lys 1 Val Met Thr Gin Cys Thr Ile Asp Arg Met Pro Glu Ala Leu Glu Leu Arg Gly Tyr Asp Ile lle Ala Leu 100 Glu Ser Arg Tyr 115 Arg Leu Pro Leu 130 Gin Glu Asp Phe 145 Glu Arg Arg Arg Lys Pro Val Leu 180 Arg Trp Pro Thr 195 Ala Gly Trp Gln 210 PCT/GB01/02003 meningitidis Ile Ile Ser Phe Ala Glu 55 Arg Ile Gly Leu Asp 135 His Ser Cys Phe Leu 215 Arg Arg 25 Pro Lys 40 Ile Leu Trp Arg Val Leu Ile Gly 105 Asn Asp 120 Arg Tyr Ser Gly Val Glu Pro Gly 185 Ala Glu 200 Phe Gly Ser Trp Lys Glu Ser Met Asn Pro Gly Arg Gly Ser Arg Thr Arg Arg Ala Leu 140 Pro Glu 155 Phe Gly Glu Phe Gly Lys Ile Leu Ala Phe Asp Leu Gly Leu 125 Ala Phe Leu Gly His 205 Gly His Val Gly Leu Lys Tyr 110 Asp His Ser Asp Pro 190 Tyr Ser Gin Asp Asp Glu Ile WO 01/85772 Ala Glu Glu 225 Gly Lys Thr Thr Val Val Gly Arg Lys 275 Pro Pro Leu 290 Ser Pro Cys 305 Asn Arg Leu Asn Leu 245 Asn Val Asp Lys Pro 325 Leu Ser Asp Gly Gin Ala Met Asp 250 Ser Gly Leu Met 265 Val Tyr Gly Ser 280 Ala Lys Ile Val 295 Glu Cys Pro Leu Lys Ile Val Gin 330 Leu Ala Ser Pro 285 Leu His 300 His Thr Val Glu PCT/GB01/02003 Cys 240 Asp Leu Thr Cys Leu 320 Val <210> 159 <211> 381 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 159 atg gaa cct tcc tcc tac gcg gca Met Glu Pro Ser Ser Tyr Ala Ala 1 5 agg cgc gtc atc aac gca ttc ggc Arg Arg Val Ile Asn Ala Phe Gly gcc tac cgt tac gaa gcg gca ttc I Ala Tyr Arg Tyr Glu Ala Ala Phe i 40 ctg ctg gtg tgc gcg gca ttt ttt 1 Leu Leu Val Cys Ala Ala Phe Phe 55 313 gga aaa Gly Lys gac ggc Asp Gly ttg tgg Leu Trp gaa aag Glu Lys ggc ggc atc Gly Gly Ile atc gcc gcc Ile Ala Ala ctg aac gcg Leu Asn Ala tec ctc cgc Ser Leu Arg WO 01/85772 ctg ccg ttg at Leu Pro Leu Il aac act gcc gtc Asn Thr Ala Val gag ctg get aaa Glu Leu Ala Lys 100 gcg atg ctg atg Ala Met Leu Met 115 <210> 160 <211> 127 <212> PRT <213> Neisseria <400> 160 Met Glu Pro Ser 1 Arg Arg Val lie Ala Tyr Arg Tyr Leu Leu Val Cys Leu Pro Leu Ile Asn Thr Ala Val Glu Leu Ala Lys 100 Ala Met Leu Met 115 t ate gcg tot ttt gtg e Ile Ala Ser Phe Val 70 Sgaa gcc gcc gtc gat SGlu Ala Ala Val Asp Scgc gcc aaa gac gca SArg Ala Lys Asp Ala 105 tta gcg gcg gtt tgg Leu Ala Ala Val Trp 120 meningitidis Ser Tyr Ala Ala 5 Asn Ala Phe Gly Glu Ala Ala Phe 40 Ala Ala Phe Phe 55 Ile Ala Ser Phe 70 Glu Ala Ala Val Arg Ala Lys Asp I Leu Ala Ala Val 120 314 I tcg Ser cat His 90 ggt Gly ctg Leu Lys 10 Ser Gn Val Ser I is 1 90 ;ly E eu gtc att gtc Val Ile Val 75 act tcg act Thr Ser Thr tct get gca Ser Ala Ala tcC gcc ctg ser Ala Leu 125 PCT/GB01/02003 gaa ctg ttc 240 Glu Leu Phe gaa aaa cac 288 Glu Lys His caa ttg ttc 336 Gln Leu Phe 110 ttc ggg 381 Phe Gly Gly Lys Asp Gly Leu Trp Glu Lys Ile Val Ser Thr Ala Ala Ala Leu 125 Gly Gly Ile Ile Ala Ala Leu Asn Ala Ser Leu Arg Glu Leu Phe Glu Lys His Gln Leu Phe 110 Phe Gly WO 01/85772 PCTIGBO1/02003 <210> 161 <211> 990 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 161 atg aaa aaa gaa ago ego ccc gog ttt gao gcg gca gcc gta ttt gac Met Lys Lys Glu 8cr Arg Pro Ala Phe Asp Ala Ala Ala Val Phe Asp gog Ala gte Val gog Ala gtc Val ego Arg tat Tyr gtg Val gca Ala ego Arg gaa Glu aag Lys aog Thr gog Ala cot Pro gee Ala Ogt Arg tgg Trp att Ile gga Gly gaa Glu. gc Ala 115 gta Val ttt Phe cag Gln atg Met aat Asn aac Asn 100 gaa Slu 009 Pro gtg Val gat Asp 050 His tog Set ego Arg ctt Leu *gta Val gao Asp ttt Phe ego Arg 70 ggc Gly gog Ala2 atc Ile toc Sex gat Asp ttt Phe 55 ccc Pro aag Lys ctt Leu gtg V~al gac Asp gaa Glu gac Asp too Ser 9gg Al a atc Ile gog Ala 120 ago Set gtc Val aaa Lys aca Thr aaa Lys gat A~sp 105 ;tt Val ggg Gly ggg Gly gcg Ala tog Ser ttt Phe 90 gat Asp ate Ile ttt Phe aaa Lys got Ala egt Arg 75 ogo Arg gtg Vfal ggg Gly gcc gac aat gee Ala Ala Asn Ala ggg gat Gly Asj tao aac Tyr Lys Cog tgg Pro Trp gge gog Gly Ala gcg oaa Ala Gln att ga Ile. Glu 125 ttt Phe 9gg Al a tat Tyr ego Arg aaa Lys 110 acg Thr too 5cr gac Asp gtg Val Cgg Arg tao Tyr aat Asn ogg Arg ato Ile tto Phe ttt Phe ggC Gly tao Tyr 144 192 240 288 336 384 ggc ana aat acg ggo agt tto cgt gtg gog gao gca ttg gog aoc tta Gly Lys 130 Asn Tin Gly Set Arg Val Ala Asp Ala Leu 140 Ala Thr Leu WO 01/85772 WO 0185772PCTIGBO1IO2003 ggc 'ttt Sly Phe 145 gat tae ccc Asp Tyr Pro Oge ege Arg Arg 150 gee ggg ttt Ala Gly Phe caa aaa gaa Gin Lys Giu ttg gte Leu Val 160 ctt tta nag Leu Leu Lys gca aaa gaa gaa ggc ggc get gtt tte gec ttt Ala Lys Giu Giu Gly Gly Asp Val Phe Ala Phe eee ggo ago Lys Gly 5cr age tac egg Tyr Arg 195 tat geg ggc gen atg ggg atg ccg eaa ttt Tyr Ala Gly Ala met Gly Met Pro Gin Phe 180 185 aae tgg geg gtg get tat gee ggg gac gga Lys Trp Ala Val Asp Tyr Asp Gly Asp Gly 200 205 atg cet teg Met Pro 190 cat egg gee Hi-s Arg Asp ate tgg gge eat gtt ggc gat gte gog gea tog Ile Trp, Gly Asn Val Gly Asp Vel Ala Ale 5cr 210 215 aeg eag eec ggt tgg ege aeg gge ggg aaa ata Lys Gin His Gly Tip Arg Thr Gly Gly Lys Ile 225 230 235 goc eat tat atg Ala Asn Tyr Met etg gtg tet gee ace Leu Vel 5cr Ala Thr 240 ttg geg ceg ggt Lou Ala Pro Gly gat gtt eag gee Asp Val Gin Ala ett ggo gee aaa le Giy Giu Lys aec gee Thr Ala 255 768 etg aeg egg Leu Thr Arg gtg gcg gat ttg Val Ala Asp Lou geg tee ggo ate Ale Tyr Gly Ile ate ccc gge Ile Pro Giy 270 gee gag ett gee get gat gaa Giu Giu Leu Ale Asp Asp Ginu 275 aeg geg gtt ttg ttc aae etg gee ace Lys Ala Val Leu Phe Lys Lou Giu Tbr 280 285 tat ttg ggc ttg eec eat ttt tat aeg Tyr Leu Gly Lou Asn Asn Phe Tyr Thr 300 gea ccc Ala Pro 290 ggc gtg ttt gee Gly Val Phe Gin tgg eag tee eat Tip Gin Tyr Asn agt egg atg tat 5cr Arg Met Tyr acg geg gte egg Thr Ala Val Arg ett gee eat teg ctt gge gge ceg gge Ile Ale Asn 5cr Lou Sly Gly Pro Sly 325 SWO 01/85772 <210> 162 <211> 330 <212> PRT <213> Neisseria PCT/GB01/02003 meningitidis <400> 162 Met 1 Ala Val Ala Val Arg Tyr Val Gly Gly 145 Glu Lys Ser Lys Ala Arg Glu Lys Thr Ala Pro Lys 130 Phe Leu Gly Tyr SLys Glu SAla Val Arg Phe STrp Gin Ile Met Gly Asn Glu Asn 100 Ala Glu 115 Asn Thr Asp Tyr Leu Lys Ser Tyr 180 Arg Lys 195 Ser 5 Pro Val Asp His Ser Arg Leu Gly Pro Leu 165 Ala Trp Arg Val Asp Phe Arg 70 Gly Ala lie Ser Arg 150 Ala Gly Ala Pro Ser Asp Phe 55 Pro Lys Leu Val Phe 135 Arg Lys Ala Val Ala Asp Glu 40 Asp Ser Ala Ile Ala 120 Arg Ala Glu Met Asp Phe Ser 25 Val Lys Thr Lys Asp 105 Val Val Gly Glu Gly 185 ryr Asp 10 Gly Gly Ala Ser Phe 90 Asp Ile Ala Phe Gly 170 Met Asp Ala Phe Lys Ala Arg 75 Arg Val Gly Asp Phe 155 Gly Pro Gly SAla Ala Gly Tyr Pro Gly Ala Ile Ala 140 Gin Asp Gin Asp Ala Ala Asp Lys Trp Ala Gln Glu 125 Leu Lys Val Phe Gly 205 Val Asn Phe Ala Tyr Arg Lys 110 Thr Ala Glu Phe Met 190 His Phe Asp Ala Asn Ser Arg Asp Ile Val Phe Arg.Phe Tyr Gly Asn Tyr Thr Leu Leu Val 160 Ala Phe 175 Pro Ser Arg Asp Ile Trp 210 Gly Asn Val Gly Asp Val Ala Ala Ser Ile 215 220 317 Ala Asn Tyr Met WO 01/85772 PCT/GB01/02003 Lys Gin 225 Leu Ala Leu Thr Glu Glu Ala Pro 290 Val Trp 305 Ile Ala Trp Ala 245 Val Asp Phe Asn Leu 325 Gly Gly Lys Ile Leu Val 235 Gin Ala Ile Ile Gly Glu 250 Leu Lys Ala Tyr Gly Ile 265 Lys Ala Val Leu Phe Lys 280 285 Tyr Leu Gly Leu Asn Asn 300 Arg Met Tyr Val Thr Ala 315 Pro Gly Leu 330 Ser Ala Lys Thr 255 Ile Pro 270 Leu Glu Phe Tyr Val Arg <210> 163 <211> 1773 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1773) <400> 163 atg agt atc gtg ctg cac ggc gtg gcg Met Ser Ile Val Leu His Gly Val Ala 1 5 ggt tgc gcc cac ctg att gcg cgc ggt Gly Cys Ala His Leu Ile Ala Arg Gly gat gtt gcg gag gcg gac acc gat gcc Asp Val Ala Glu Ala Asp Thr Asp Ala 40 gcc gtc aaa gcc acg cgc aaa gag ttg Ala Val Lys Ala Thr Arg Lys Glu Leu 318 gcg ggc Ala Gly acg gag Thr Glu gaa gcc Glu Ala gaa cag Glu Gln aaa ggc Lys Gly gaa gtg Glu Val gaa cgt Glu Arg ctc cgc Leu Arg WO 01/85772 PCT/GB1/02Oo3 gaa aao gc cog acc gag ttg ggo geg tto ato tog etg cac Giu Asn Ala Pro Thr Giu Leu Gly Ala Phe Ile 3cr Leu His 240 atg ctc ttg ace Met Leu Leu Thr gat gtt Asp Val ace ttg tog cgo gaa ccc gte gat Thr Leu Ser Arg Giu Pro Val Asp att tta Ile Leu agg gaa caa Arg Giu Gin atc aac gc gag tgg gca ttg aag Cag cag ago gac Ile Ann Ala Giu Trp Ala Leu Lys Gin Gin Ser Asp aaa oto gcc gee cas tto gac LyS Leu Ala Ala Gin Phe Asp 115 atg gac gat goc tat ttg cgo gaa Met Asp Asp Ala Tyr Leu Arg Giu 125 cgc aag Arg Lys 130 cag gat atg Gin Asp Met ctg eaa Leu Gin 135 gtc gtc ogc cgc Val Vai Arg Arg cac aao aac otg His Ann Ann Leu ggg cag ggc aac Gly Gin Gly Asn ttg gaa gtt gec Leu Giu Val Ala aao ctg ttt gao Ann Leu Phe Asp ace gtt otg att Thr Val Leu lie aac gac ctt tcg Asn Asp Leu Set gc gao acg gtt Ala Asp Thr Val ttg ttt Leu Phe 175 aaa gag cag Lys Giu Gln ggg oat acg Gly His Thr 195 att gec gc ttc gtt ace gat goo ggc Ile Ala Ala Phe Vai Thr Asp Ala Gly 185 ggo ccc aco Gly Pro Thr 190 tee gtc gtc Ser Val Vai gcg att ttg Ala Ile Len ggo agg Gly Arg 200 aaa ctg Lys Leu '215 ago ttg gac ate Ser Len Asp Ile ggg ctg Sly Leu 210 gtg gac Val Asp 225 cac aac gog cgc His Ann Ala Arg att acc gag lie Thr Glu gaa aog gto att Glu Thr Val Ile ggt ato aac ggo gtg ttg att Gly Ile Asn ely Val Leu Ile 230 ate gog Ile Ala 235 ccg gat gag Pro Asp Glu tcg gtg Ser Val 240 ttg nac gaa tac cgc cgc cgt gc cgc gaa tac 090 ago cac aaa cgo Leu Asn Glu Tyr Arg Arg Arg Ala Arg Glu Tyr Arg Ser His Lys Arg WO 01/85772 PCTIGB01/02003 gat ttg aac Asp Leu Asn ctc aaa aaa acc gcc gee gee ace got Leu Lys Lys Thr Ala Ala Ala Thr Ala 265 tgc ate gag ctt Cys Ile Glu Leu 275 gtg ggc aat ata gaa tcc gee Val Gly Asn Ile Glu Ser Ala gaa gac Glu Asp 285 tte egc Phe Arg 300 gac ggg gtc Asp Gly Val 270 gtg aaa ccg Val Lys Pro age gag ttt Ser Glu Phe ctg cac Leu His 290 aac ctc ggc gea Asn Leu Gly Ala ggc ate ggg ctg Gly Ile Gly Leu tac ctg aac cge Tyr Leu Asn Arg acg atg ccg tot Thr Met Pro Ser gac gag cag tac Asp Glu Gln Tyr 912 960 1008 gtg tac age gcg Val Tyr Ser Ala gtc aaa aaa atg Val Lys Lys Met ggc aaa age gta Gly Lys Ser Val acg ata Thr Ile 335 egg aca gtc Arg Thr Val aac age acg Asn Sex Thr 355 gat Asp 340 ttg ggt gtg gac Leu Gly Val Asp aac ccg cge tgg Ann Pro Arg Trp ttc ggg aaa Phe Gly Lys 350 atg ace ggc Met Thr Gly 1056 1104 ccc aac ggc age Pro Asn Gly Ser aac ccc gcg ctg Aan Pro Ala Leu ate cge Ile Arg 370 ctg tgc ctt gee Leu Cys Leu Ala ccg gtc atg tte Pro Val Met Phe acc cag atg cgc Thr Gln Met Arg ate etc cge gcc Ile Leu Arg Ala gee cac ggc ccc Ala His Gly Pro cgg atg atg tgg Arg Met Met Trp 1152 1200 1248 atg att ace tcc Met Ile Thr Ser tcc gaa gtg cgc Ser Glu Val Arg tgc ctc atc cac Cys Leu Ile His ctc gac Leu Asp 415 ace gcg caa cge cag ctt gee gaa Thr Ala Gln Arg Gln Leu Ala Glu 420 gge atc ggc tgt atg att gaa att Gly Ile Gly Cys Met Ile Glu Ile cge ggc gat gee Arg Gly Asp Ala 425 ttc ggt aaa gtc Phe Gly Lys Val 430 1296 ccg tct gcc gcg ctg ace gtc ggc Pro Ser Ala Ala Leu Thr Val Gly 1344 WO 01185772 WO 0185772PCTIGBO1IO2003 agt att ttg aaa otg Se Ile Leu Lys Leu gto gat tto Val Asp Phe ato too gto Ile Ser Val ggt aco Gly Thr 460 aao gao otg Asn Asp Leu 1392 oaa tao ato ttg Gin Tyr Ile Leu gto gat cgc ggc Val Asp Arg Gly gao gao ago gto Asp Asp 5cr Val 475 aaa atg otg ona Lys Met Lou Gin ago cac Set His 480 oao gtc His Val 495 1440 oto tao oag 000 Leu Tyr Gin Pro cat ooo goo gtg His Pro Mla Vai 1488 atc cgt aco gcc aao ago atcj gao aaa Ile Arg Thr Mla Asn Arg Met Asp Lys 500 505 atg gcg ggc gat aoo gog ttt aoc ogo met Mla Gly ASP fit Mla Phe Thr Arg 515 520 gao gta too gta Asp Val 8cr Val tgo ggc gag Cys Gdy Giu 510 atg ggg otg Met Gly Lou 1536 1584 gtt tta ttg Val Leu Leu ogo ogt Axrg Mxg 530 ttt too atg aac Phe 8cr met Asn aao aao ato otg Asn Asn Ile Leu gto aaa aac ato Val Lys Aan Ile otg oao ago ant Lou His 8cr Asn gga cag cto gaa Gly Gin Leu Giu gat att gtg aaa Asp Ile Val Lys 1632 1680 1728, ato ogo tgo gaa Ile Axg Cys Glu gaa gag nag tog Glu Glu Lys Set nag otg ato aaa, Lys Leu Ile Lys cag atg Gin Met 575 aao ago gtg tot gtc gag gan gaa Asn 5cr Val Set Vai Glu Giu Giu 580 <210> 164 <211> 591 <212> PRT <213> Neisseria ineningitidis <400> 164 Met Set Ile Val Lax His Sly Val gao tto aag ggg Asp Phe Lys Sly ogg aaa Arg Lys 590 1773 Mla Mla Giy Lys Gly Ile Ala Val WO 01/85772 Gly Cys Ala PCT/GBOI/02003 His Leu Ile Ala Arg Gly Thr Gl Giu Val Pro Sin Tyr Asp Val Ala Val Pro GLu Met Leu Arg Glu Lys Lea Arg Lys 130 Ile Gly 145 Thr Val Lys Giu Gly His Gly Lea 210 Val Asp 225 Leu Asn Ala Lys Asn Lea Gin Ala 115 Gin Gin Lea Gin Thr 195 His Gly Alu Gli Ala Ala Thx Lys 100 Ala Asp Gly le Arg 180 Ala Asn Ile Tyr 1 Ala Thr Pro Asp lie Gin Met Asn Ala 165 lie Ile Ala Asn Arg I 245 Asp Arg Thz 70 Val Asn Phe Leu Glu 150 Asn Ala Lea Erg 3iy 230 krg Thr Lys 55 Glu Thr Ala Asp Gin 135 Leu Asp Ala ely. Lys 215 Val Arg I Asp 40 Glu Lea Lea Glu Asn 120 Val Glu Lea Phe Arg 200 Lea Lea kla Ala Leu Sly Ser Trp 105 Met Val Val Ser Val 185 Ser Ile Ile Arg 4 Glu Ala Glu Arg Phe Asp Ala Glu Ala Arg 90 Ala Asp Arg Ala Pro 170 Thr Lea Thr lie Glr Phe 75 Glu Let Asp Arg Asp 155 Ala Asp Asp Glu Ala 235 Tyr 1 Lea Ile Pro Lys Ala Ile 140 Asn Asp Ala lie Gly 220 Pro Arg Arg Ser Val Gin Tyr 125 His 1,eu Thr Gly Pro 205 Glu Asp Ser Sea Let Asp Gin 110 Lea Asn Phe Val Gly 190 Ser Thr Glu His Ala His I Lie Ser Arg Asn Asp Lea 175 Pro Val Val Ser Lys I 255 Ile Leu Leu Asp Glu Lea Glu 160 Phe Thr Val ile fal klrg Asp Lea Asn Leu Lys Lys Thr Ala Ala Thr Ala Asp Gly Val 270 WO 01/85772 Cys lie Glu 275 PCT/GB01/02003 Leu Val Gly Asn lie Glu Ser Ala Gli Asp Val Lys Pro 280 285 Leu Leu 305 Val Arg Asn lie Ala 385 Met Thr Gly Ser lie 465 Leu Ile Met i His Asn 290 Tyr Leu Tyr Ser Thr Val Ser Thr 355 Arg Leu 370 lie Leu lie Thr Ala Gin Ile Gly 435 lie Leu 450 Gin Tyr Tyr Gln Arg Thr Ala Gly I 515 Leu Asn Ala Asp 340 Pro Cys Arg Ser Arg 420 Cys Lys Tle Pro Ala 500 Asp SGly Ala SArg Asp 310 Ile Val 325 Leu Gly Asn Gly Leu Ala Ala Ala 390 Val Ser 405 Gln Leu Met Ile Leu Val Leu Ser 470 Gly His 485 Asn Arg Thr Ala Asi 295 Thr Lys Val Ser Glu 375 Ala Glu Ala Glu Asp 455 Val Pro Met Phe SGly SMet SLys Asp Leu 360 SPro His Val Glu Iie 440 Phe Asp Ala Asp Thr 1 520 Ile Pro Met Lys 345 Asn Val Gly Arg Arg 425 Pro Ile Rrg Val Lys 505 Arg Gly Ser Lys 330 Asn Pro Met Pro Gin 410 Gly Ser Ser Gly Leu 490 Asp Val Leu Phe 300 Glu Asp 315 Gly Lys Pro Arg Ala Leu Phe Arg 380 Val Arg 395 Cys Leu Asp Ala Ala Ala Val Gly 460 Asp Asp 475 Lys Met Val Ser Leu Leu Arg Glu Ser Trp Gly 365 Thr Met Ile Phe Leu 445 Thr Ser Leu Val Gly 525 SSer Gln Val Phe 350 Met Gln Met His Gly 430 Thr Asn Val Gin Cys 510 Met Glu Tyr Thr 335 Gly Thr Met Trp Leu 415 Lys Val Asp Ser His 495 Gly Gly Phe Glu 320 Ile Lys Gly Arg Pro 400 Asp Val Gly Leu His 480 Val Glu Leu WO 01/85772 PCT/GB01/02003 Arg rg Phe Set Met Asn Pro Asn Asn Ile Leu Pro Val Lys Asn Ile 530 535 540 Ile Leu His Ser Asn Val Gly Gin Leu Glu Ser Asp Ile Val Lys Val 545 550 555 560 Ile Arg Cys Glu Asp Glu Glu Lys Ser Glu Lys Leu Ile Lys Gin Met 565 570 575 Asn Set Val Ser Val Glu Glu Glu Ala Asp Phe Lys Gly Arg Lys 580 585 590 <210> 165 <211> 381 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 165 atg gaa cct tec tcc tac gcg gca gaa aaa aaa gga aaa gge gge ate 48 Met Glu Pro Ser Ser Tyr Ala Ala Glu Lys Lys Gly Lys Gly Gly Ile 1 5 10 agg cgc gtc atc aac gca ttc ggc tat tcg ata gac ggc ate gcc gcc 96 Arg Arg Val Ile Asn Ala Phe Gly Tyr Ser Ile Asp Gly Ile Ala Ala 25 gcc tac cgt tac gaa gcg gca ttc cgt cag gtt ttg tgg ctg aac gcg 144 Ala Tyr Arg Tyr Glu Ala Ala Phe Arg Gln Val Leu Trp Leu Asn Ala 40 ctg ctg gtg tgc gcg gca ttt ttt tgg gtt tcc gaa aag tcc atc cgc 192 Leu Leu Val Cys Ala Ala Phe Phe Trp Val Set Glu Lys Set Leu Arg 55 ctg ccg ttg att ate gcg tct ttt gtg tcg gtc att gtc gaa ctg ttc 240 Leu Pro Leu Ile lie Ala Ser Phe Val Set Val Ile Val Glu Leu Phe 70 75 aac act gcc gtc gaa gcc gcc gtc gat cat act tcg act gaa aaa cac 288 Asn Thr Ala Val Glu Ala Ala Val Asp His Thr Ser Thr Glu Lys His 90 WO 01/85772 PCT/GB01/02003 gag ctg get aaa cgo gcc aaa gac gca ggt tot get gca caa ttg ttc 336 Glu Leu Ala Lys Arg Ala Lys Asp Ala Gly Ser Ala Ala Gln Leu Phe 100 105 110 gcg atg ctg atg tta gcg gcg gtt tgg ctg tcc gcc ctg ttc ggg 381 Ala Met Leu Met Leu Ala Ala Val Trp Leu Ser Ala Leu Phe Gly 115 120 125 <210> 166 <211> 127 <212> PRT <213> Neisseria <400> 166 Met Glu Pro ser 1 Arg Arg Val Ile Ala Tyr Arg Tyr Leu Leu Val Cys Leu Pro Leu Ile Asn Thr Ala Val Glu Leu Ala Lys 100 Ala Met Leu Met 115 meningitidis Ser Tyr Ala Ala Asn Ala Phe Gly Glu Ala Ala Phe 40 Ala Ala Phe Phe Ile Ala Ser Phe Glu Ala Ala Val Arg Ala Lys Asp Leu Ala Ala Val 120 Lys Gly Trp Lys Val Thr Ala Leu 125 <210> 167 <211> 1257 <212> DNA <213> Neisseria meningitidis <220> <221> CDS WO 01/95772 <222> (1257) <400> 167 PCT/GBO1/02003 atg Met 1 gtt Val 9gg Ala gec Ala cag Gin ttg Leu gtg Val tac Tyr gac Asp otg Leu 145 tgg Trp, agt atg 5cr Met 900 gaa Ala Giu cat ccc His Pro tao ggo Tyr Gly atg otg Met Leu gog gog Ala Ala gto aac Val An ogt tog Arg 3cr 115 aag ctt Lys Leu 130 cog otg Pro Leu cac aao His An goa Ala gga Gly gac Asp tgc Cys aaa Lys ttg Leu gaa Giu 100 ttt Phe 909 Ala tgg rrp ate Ie ott goc oaa aaa ott gcc gcc gao ago att gog gog Leu Ala Gin LYS Leu Ala Ala Asp 5cr Ile Ala Ala 5 090 Arg ott Leu cag Gin aag Lys tao Tyr got Ala g00 Ala got Al a tgg Trp gc Ala 165 aao Asn aog Thr ogt Arg cocg Pro 70 ona Gin gtt Vai aao An too Set gtg Val 150 geo Ala ott Leu gog Ala tat Tyr 55 att le otg Leu gaa Glu gog Ala tgo Cys 135 got Ala 909 Ala Cag Gin cag Gin 40 ttg Leu 990 Gly oat His ago Ser att Ile 120 aaa Lys tao Tyr ctg Leu gao Asp 25 gaa GIU Gly aat Asn tao Tyr ae Ile 105 ttg Leu aaa Lys ttg Leu oaa Gin 10 gtg Val sac Asn agt Ser cog Pro aog flit 90 goc Ala ogo Arg gao Asp aaa Lys too Set 170 ttg gog Leu Al a ggo gg Gly Ala ttg aaa Leu Lys cag oto Gin Leu 75 c90 eac Arg An aaa ato Lys Ile ogo ttt Mrg Phe gat gtg Asp Val 140 aao oat Asn His 155 cat cog His Pro oaa Gin ttg Leu oat His gaa Giu gog Ala gga Gly ttg Leu 125 gog Ala tat Tyr cog Pro ato le cag Gin atg met agt Set cog Pro ogo Mrg 110 090 Mrg aaa Lys cog Pro atg M1et ogo Mrg gao Asp oto Leu otg Leu oat His 999 Gly gaa Giu cac His aaa Lys aog Thr 175 aco Tbr ato Ile g9 Ala ott Leu got Ala cag Gin ogo Arg ac An cao His 160 ttg Lou 96 144 192 240 288 336 384 432 480 528 ogo gto eec ogo oga oao ggc eac goc gee ago tat ttg gae sea ctg WO 01/85772 AMg Val Asn Arg 180 Arg His Gly Asn Ala Glu 5cr Tyr Leu Glu Lys PCT/GBO1/02003 Leu get Ala ttg Leu att Ile 225 ac Asn gge Gly ttg Leu egt Arg ttg Leu 305 gtg Val tgg Trp gec c Ala I geo Als gas Git 210 gtt Val aeg Pro ag Lys gac Asp ttg Leu 290 ge-g Ala Pro :tg 1 eu :tg .eu Igui tGi 195 Lgas G Si *tag *Ser *aaa Lys aeg Thr att le 275 gge Gly geg Ala tga Cys ego Arg ctg Leu gge aGly gee IAla gta Val gac Asp ggg Gly 260 gat Asp ttt Phe tggI Trp acec Thr egt c Arg F 340 gac g Asp ati Ile gtc VaJ eac Glr ggc Giy 245 eat His gee Ala cog Gln tat Cyr let 325 zag 'ro rca tgag e Ala I j LPro Sgoc IAsp 230 gaa Glu ate Ile Gly. ag Thr gat Asp 310 tea Bar acec Thr ctt t Lou T ga Ala gtg Val 215 tte Phe egg Arg ttg Lou cgt Arg gee Ala 295 gga fly: fly I jac c Isp rp C aol Ly: 203 taa Ama G13 att Ile gaa Giu ctg Lou 280 tag 5cr geg S Al. 0 egc An goc Ala *ttg Leu etg Leu 265 aag Lys gag Ala cat Pro gag Ala eta Leu 345 aag Thr gttg a Leu -ctg ;Leu cog Gin gac IAsp 250 gag *Ala egt Arg geg Ala ttc Phe Arg 330 aaa a Lys 3I etg a Lou L Asj Prc cac Gir 235 gag Ala gat Asp gtg Val tgt Cys gac 315 :ge Xtg Yhr 100 'Ys c gaa p Glu 2ggt 3 Gly 220 gag Ala Itge Cys tgc Cys gag Giu gee Ala 300 aca Thr aat Asn gao AlaI aag E Gin tat Tyr 205 ttt Phe gag Ala gee Ala age Arg gac Asp 285 got Asp ate Ile :zec Pro :ge krg jgo 1y 190 gag gtt acg Ala Vol Thr tea gac gge Set Asp Gly tat ata att Tyr Lou Lou 240 gag eog gge Ala Pro Gly 255 gtt ace gc Val Thr Ala 270 aat ate gee Asn le Ala gag egg got Ala Mrg Asp 624 672 720 768 816 864 912 960 1008 1056 1104 gee Ala gtg Val 335 cog Gin aga Arg 355 365 ctg ett gee ace tgt tee gtg tta gte qua gaa aoe gac ggg coo ttg 327 1152 WO 01/85772 Leu Leu Ala 370 caa aaa ttc Gin Lys Phe 385 gtg ctt tta Val Leu Leu caa aag cat Gin Lys His <210> 168 <211> 419 <212> PRT <213> Neisse PCT/GB01/02003 Thr Cys Ser Val Phe Val Glu Glu Asn Asp Gly Gln Leu 375 380 etc aac cgc cat gcc gat gcc gaa ccg atc gaa tcg egg 1200 Leu Asn Arg His Ala Asp Ala Glu Pro Ile Glu Ser Arg 390 395 400 ccg aac aaa cac caa gat ggc ttt tat tac gcg ctt att 1248 Pro Asn Lys His Gln Asp Gly Phe Tyr Tyr Ala Leu lle 405 410 415 1257 ria meningitidis <400> 168 Met Ser 1 Val Ala Ala His Ala Tyr Gln Met Leu Ala Val Val Tyr Arg Asp Lys 130 Met Ala Glu Gly Pro Asp Gly Cys Leu Lys Ala Leu Asn Glu 100 Ser Phe 115 Leu Ala Gin Leu Ala Tyr 55 Ile Leu Glu Ala Cys 135 Leu Ala Ala Asp Ser Ile Ala Ala 10 Asp Val 25 Glu Asn Gly Ser Asn Pro Tyr Thr Ile Ala 105 Leu Arg Ala Gin Ile Arg Thr Ala Leu'Gln Asp Ile Lys His Met Leu Ala Leu Glu Ser Leu Leu Asn Ala Pro His Ala Ile Gly Arg Gly Gln 110 Phe Leu Arg Glu Arg 125 Val Ala Lys His Asn 140 Lys Asp Asp WO 01/85772 Leu Pro Leu 145 Trp His Asn Arg Val Asn Ala Ala Glu 195 Leu Glu Glu 210 Ile Val Ser 225 Asn Pro Lys Gly Lys Thr Leu Asp Ile 275 Arg Leu Gly 290 Leu Ala Ala 305 Val Pro Cys Trp Leu Arg Ala Leu Leu 355 Leu Leu Ala 370 Gin Lys Phe 385 Trp Trp Ile Ala 165 Arg Arg 180 Gly lie Ala Val Val Gin Asp Gly 245 Gly His 260 Asp Ala Phe Gin Trp Tyr Thr Ala 325 Arg Pro 340 Asp Ala rhr Cys Leu Asn Val 150 Ala His Ala Pro Asp 230 Glu lie Gly Thr Asp 310 Ser Thr Leu Ser Arg 390 Ala Ala Gly Ala Val 215 Phe Arg Leu Arg Ala 295 Gly Gly Asp Trp Val 375 His Tyr Leu Leu Gin Asn Ala 185 Lys Ala 200 Asn Arg Gly Ala Ile Leu Glu Leu 265 Leu Lys 280 Ser Ala Lys Pro Val Ala Ala Leu 345 Gin Thr 360 Phe Val Ala Asp Lys Ser 170 Glu Leu Leu Gin Asp 250 Ala Arg Ala Phe Arg 330 Lys Leu Glu Asn 155 His Ser Asp Pro Gln 235 Ala Asp Val Cys Asp 315 Arg Thr Lys Glu His Pro Tyr Glu Gly 220 Ala Cys Cys Glu Ala 300 Thr Asn Ala Gin Asn 380 Tyr Pro Leu Tyr 205 Phe Ala Ala Arg Asp 285 Asp Ile Pro Arg Gly 365 Asp Pro Met Glu 190 Ala Ser Tyr Ala Val 270 Asn Ala Leu Asp Gin 350 Gly Gly Lys Thr 175 Lys Val Asp Leu Pro 255 Thr Ile Arg Ala Val 335 Gin Arg Gin PCT/GB01/02003 His 160 Leu Leu Thr Gly Leu 240 Gly Ala Ala Asp Asp 320 Lys Glu Met Leu Ala Glu Pro Ile Glu Set Arg WO 01/85772 PCT/GB01/02003 Val Leu Leu Pro Asn Lys His Gln Asp Gly Phe Tyr Tyr Ala Leu Ile 405 410 415 Gln Lys His <210> 169 <211> 804 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 169 atg Met 1 gcg Ala gca Ala ccc Pro gat Asp aaa Lys gcg Ala tot aaa ys gcc Ala gac Asp tot Sex aac Asn ccg Pro gtg Val 100 ttc tgg Trp ggc Gly gtg Val gac Asp atg Met 70 gac Asp tcg Ser gac gcc gcc gcc Ala Ala Ala 10 cag ggt aaa Gin Gly Lys cgc gtg got Arg Val Ala aaa ggc aat Lys Gly Asn aag gcg ggc Lys Ala Gly ctt ttc ccC Leu Phe Pro gta acc att Val Thr Ile 105 tat ttt gaa gcg ctc Ala Leu ccc gcc Pro Ala ttt gcc Phe Ala gat gtg Asp Val gaa ttc Glu Phe ggc gat Gly Asp aaa cag Lys Gln gtc ctc Ser Met Asp Phe Ser Asp Pro Tyr Phe Glu Ile Thr Gln Val Val Leu WO 01/85772 PCT/GB01/02003 gtt ccg aaa Val Pro Lys 130 ggc aaa aaa Gly Lys Lys tot tct tcc gaa gat ttg aaa aac atg Sex Set Ser Glu Asp Leu Lys Asn Met aac aaa gtc ggc gtg Asn Lys Val Gly Val 145 aaa ctc ttg ggc aac Lys Leu Leu Gly ASn 165 acc ggc tac acg Thr Gly Tyr Thr gat ttc tooc gta Asp Phe Ser Val gac aac ccg aaa Asp Asn Pro Lys gcg cgc ttt gas Ala Arg Phe Glu aac gtt Asn Val 175 ccc ctg att Pro Leu Ile agc gac agc Ser Asp Set 195 aaa gaa ctg gaa Lys Giu Leu Glu ggc ggc ttg gat Gly Gly Leu Asp tcc gtg gtc Ser Val Val 190 ccg acc aaa Pro Thr Lys gca gtc atc gcc Ala Val Ile Ala tat gtg aaa aac Tyr Val Lys Asn ggg atg Gly Met 210 atc gcg Ile Ala 225 gcg ttg Ala Leu gac ttc gtt acc ctg ccc gac ttc acc Asp Phe Val Thr Leu Pro Asp Phe Thr 215 gaa cac tac ggc Glu His Tyr Gly gta cgc aaa Val Arg Lys aaa aaa gta Lys Lys Val 245 gac gaa gca acc Asp Glu Ala Thr aaa atg ctg aac Lys Met Leu Asn cge gaa agc ggc Arg Glu Set Gly tac gac aaa ate Tyr Asp Lys Ile tac gcc Tyr Ala 255 aaa tat ttt gca aaa gaa gac gga cag gcc gca aaa Lys Tyr Phe Ala Lys Glu Asp Gly Gln Ala Ala Lys 260 265 <210> 170 <211> 268 <212> PRT <213> Neisseria meningitidis <400> 170 Met Asn Met Lys Lys Trp Ile Ala Ala Ala Leu Ala Cys Ser Ala Leu 1 5 in 1I WO 01/85772 Ala Leu Ser PCT/GB01/02003 Pro Ala Ala Cys Gly Gly Gin Gly Lys Asp Ala Ala Ala Ala Pro Asp Lys Ala Ser Val Asn 145 Lys Pro Ser Gly lle 225 Ala Asn Phe Leu His Asp Met Pro 130 Lys Leu Leu Asp Met 210 Ala Leu Pro Glu Met Gin Val Asp 115 Lys Val Leu Ile Ser 195 Asp Val Lys SAsp Ser Asn SPro Val 100 Phe Gly Gly Gly Ile 180 Ala Phe Arg Lys Ala 260 Lys Leu Ala Trp Met Ser Lys Val Asn 165 Lys Val Val Lys Val 245 SVal Asp Met 70 Asp Ser Asp Lys Val 150 Asp Glu Ile Thr Gly 230 Arg Tyr Ser 55 Ala Set Gly Pro lle 135 Thr Asn Leu Ala Leu 215 Asp Glu Arg 40 Lys Lys Leu Val Tyr 120 Ser Gly Pro Glu Asn 200 Pro Glu Ser Val Ala Gly Asn Ala Gly Phe Pro 90 Thr Ile 105 Phe Glu Ser Ser Tyr Thr Lys Ile 170 Asn Gly 185 Tyr Val Asp Phe Ala Thr Gly Glu 250 Gin Ala 265 Set Val Asn 75 Ala Thr Ile Glu Gly 155 Ala Gly Lys Thr Val 235 Tyr Ala Asn Ala Glu Gly Phe Lys Leu Asn Asp Asp Thr Gln 125 Asp Leu 140 Asp Phe Arg Phe Leu Asp Asn Asn 205 Thr Glu 220 Lys Met Asp Lys Lys Gl. Phe Ile Asn Arg 110 Val Lys Ser Glu Ser 190 Pro His Leu Ile Phe Ala Asp Val SGlu Phe Gly Asp Lys Gin Val Leu Asn Met Val Ser 160 Asn Val 175 Val Val Thr Lys Tyr Gly Asn Asp 240 Tyr Ala 255 Lys Tyr Phe Lys Glu Asp Gly WO 01/85772 <210> 171 <211> .864 <212> DNA <2 13> Neisseria meningitidis <220> <221> CDS <222> (864) PCT/GBO1/02003 <400> 171 atg aaa gca aaa Met Lys Ala Lys 1 agc ctg ttt gcc LCU Phe Ala agt too gtc att 5cr Val Ile cgt goc gaa gao Arg Ala Glu Asp gtg gtc aac acc Val Val Asn Thr cgg tcg gca gag A-rg 8cr Ala Glu ass, aeg tog ggo Lys Lys 8cr Gly 100 gog gta a tat Ala Val Lys Tyr 115 atc ctg act too gtt gca ctg ott gcc tgt too ggc Ile Leu Thr 8cr Val Ala Leu Leu Ala Cys 5cr Gly acg ctg gca Thr Lcu Ala gcg cag gtt Ala Gin Val ccg caa ctg Pro Gin Lcu gtc gca cag Val Ala Gin ae sat gcg Lys Asn Ala gao eag aaa Asp Lys Lys ttg sac ggc Leu Asn Gly 120 toc gag cag aac ggt cag ac etc gac Asn Gly Gin Lys Ile Asp 144 192 240 288 336 384 432 aaa. ecc caa Cog gtt gee gta aes goc gc tat gac act Lys Thr 130 Gin Pro Val 8cr Gin Glu Vel Lys Ala Tyr Asp Asn WO 01/85772 PCT/GB01/02003 agc ggt ttt tac Sex Gly Phe Tyr ggt acg cag gaa gtc cag ttg ggc gaa.atc Gly Thr Gin Glu Val Gin Leu Gly Glu Ile ctg acc gac aag Leu Thr Asp Lys gaa aat gca Glu Asn Ala aaa aaa Lys Lys .170 ttg aaa Leu Lys 185 gcg gtt gcc Ala Val Ala caa tat tcc Gln Tyr Ser gcg aaa aaa Ala Lys Lys ttc gat gcc gtc Phe Asp Ala Val gac ttg aag Asp Leu Lys 175 ctc aac gac Leu Asn Asp 190 ctg aaa gat Leu Lys Asp cgt acc aaa cag acc ggt gcg Arg Thr Lys Gin Thr Gly Ala gtc gga tat gtg Val Gly Tyr Val ttg gaa Leu Glu 210 cag ggt gtt Gln Gly Val ccg cog Pro Pro 215 ctt tat cag gca Leu Tyr Gin Ala aag gac ttg aaa Lys Asp Leu-Lys ggc gaa ttt acg Gly Glu Phe Thr acg ccg ctg aaa Thr Pro Leu Lys ggc gat ttc tac Gly Asp Phe Tyr gtt tat tat gtc Val Tyr Tyr Val gac agc cgc gag Asp Ser Arg Glu aaa gtg cct tot Lys Val Pro Ser ttt gat Phe Asp 255 gaa atg aaa Glu Met Ly cgt goc gtc Arg Ala Val 275 cag att gcg ggc Gin Ile Ala Gly ctt cag gcg gaa Leu Gln Ala Glu cgg att gac Arg Ile Asp 270 cct gca aaa Pro Ala Lys ggt gca ctg ttg Gly Ala Leu Leu aag gca aac atc Lys Ala Asn Ile <210> 172 <211> 288 <212> PRT <213> Neisseria <400> 172 Met Lys Ala Lys meningitidis Ile Leu Thr Ser Val Ala Leu Leu Ala Cys Ser Gly Thr Val Asn Gly Gin Lys Ile Asp 25 Set Leu Phe Ala Gin Thr Leu Ala WO 01/85772 Set Ser Val PCT/GB01/02003 Ile Asp Ala Gln Val Ala Ala Phe Arg Ala Glu Asn Ser 40 Arg Val Arg Lys Ala Lys Ile 145 Leu Ala Arg Leu Lys 225 Ala Val Ser Lys Val Thr 130 Ser Thr Lys rhr Glu 210 Gly Glu Asn Ala Ser Lys 115 Gin Gly Asp Lys Lys 195 Gin Gu Asp Thr Glu Gly 100 Tyr Pro Phe Lys Gly 180 Gln Gly Phe Thr Val Phe Asp Gly Val Tyr Glu 165 Phe Thr Val Thr SPro Glr 5! Val Ale 70 Lys Asr SAsp Lys Leu Asn Set Glu 135 Lys Gly 150 Glu Asn Asp Ala Gly Ala Pro Pro 215 Ala Thr 230 Asp Ser 1 Leu Arg Gin Glu i Ala Leu Lys Pro 105 Gly Glu 120 Gin Glu Thr Gin Ala Lys Val Leu 185 Pro Val 200 Leu Tyr Pro Leu Arg Glu I Gin Val Ala 90 Ser Ala Val Glu Lys 170 Lys Gly IGn I Lys I ral I 250 Leu Sei Lys 75 Lys Phe Tyr Lys Val 155 Ala Gln Tyr Ala sn 235 Lys ;ln r Lei 6( SAr SLet SLys Ale Ala 140 Gin Val Tyr Val Ile 220 Gly Val Ala J Leu 0 g Leu a Arg SThr Leu 125 SAla Leu Ala Ser Pro 205 Lys Asp ,Pro Glu Glu Lys Ala Val 110 His Tyr Gly Asp Leu 190 Leu Asp Phe Ser 0xg 270 SAsn Leu Glu Trp Ile Asp Glu Leu 175 Asn Lys Leu Tyr Phe I 255 Ile I Glu Asp Ala Gin Ala Asn Ile 160 Lys Asp Asp Lys Gly 240 Asp Asp Val Tyr Tyr Val Asn 245 2 Glu Met Lys Gly Gin Ile Ala Gly Asn 260 265 Arg Ala Val 275 Gly Ala Leu Leu Gly Lys Ala Asn Ile Lys 280 285 335 Pro Ala Lys WO 01/85772 WO 0185772PCT/GBOI/02003 <210> 173 <211> 1794 <212> DNA <213> Neisacria meningitidis <220> <221> CDS <222> (1)..(1794) <400> 173 ttg ccc cga att gco atg ccc tat ttc goc ctg ttt gao' gat goc gta Leu Pro Arg Ile Ala Met Pro Tyr Phe Ala Leu Phe Asp Asp Ala Val agc cgt Mrg caa G-in cog Pro tgg Trp 050 His too ggt oge Gly Mrg cCC ga Pro Glu aaa ggg Lys Sly ctg aog Leu Tin ttt gc Phe Ala tca gac 5cr Asp gas gc Giu Ala 115 gca Ala gas G1w ctg Lcu ggg Gly gac Asp ggc Sly 100 gat Ap ass ogo tat osa sat cat gtg Lys Arg Tyr Gin Asn His 'Val 25 otc cat His gtc Val tgc Cys ctc Leu tao Tyr gat Asp goc Ala gag Glu 70 goc Ala coo Pro oto Lcu got Ala gtg Val. 55 tcc 5cr gac Asp gcc Ala gac Asp ttg gao ggc gog Leu 40 ttg Leu gas G1w sac Thr ggo Sly oat His 120 Gly gca Al a ggc Gly Ala 90 too Scr ego Mrg Ala gac Asp ggc Gly 75 gee Ala Thr caa Gin gee ctg Leu tac Tyr ac Asn ago Set oog Pro ago Ser caa Gin gga Sl1y ott Lcu tgg Trp cas Gin ogt Arg tog Set ttc Pile gc Ala ott Lcu too Ser ttt Pile Gly ggt Giy ctg Lcu goo Ala too 5cr ttc Pile tgg Ttp ttg Lcu ceo His cga Arzg ;ta Val 96 144 192 240 288 336 110 oga oge ggo gao aoc tat osa ate sac tao act Mrg Mg Gly Asp Thr Tyr Gin 130 135 Ile Asn Tyr Tilt Thr 140 336 Mrg Lew His Lcu WO 01/85772 PCT/GBO1/02003 caa gee tac gge aat ccc gtc aan ett tac ena ege ctg ege cag ec Gin Ala Tyr Gly Asn Pro Val Lys Leu Tyr Gin Arg Leu Arg Gin Pro 155 160 gtc ccc tat gcc gtt ttg tec eac etg ccc gat gca eaa ggg caa tcc Val Pro Tyr Ala Val Leu Ser His Leu Pro Asp Ala Gin Gly Gin geg tgg aeg Ala Trp Thr gac gge ac ASP Gly Thr 195 tgt ttc teg ccc gaa etc tte etc aaa ate ggt tcg Cys Pbs Sex Pro Giu Leu Phe Leu Lys Ile Gly ate agc ace gaa Ile 5cr Thr Giu atg aaa gge ac Met Lys d-Y Tin ecg att ttg Pro Ile Lcu gge gee Gly Asp 210 gga caa gee gee Gly Gin Asp Giu ege gee gee gag Arg Ala Ala Giu caa gea gac ceg Gin Ala Asp Pro *aae ege gee gaa Asn Arg Ala Giu gtg atg att gte gat ttg etg egt aac Val Met Ile Val Asp Leu Leu Arg Asn 720 768 etc ggc aan ate Leu Gly Lys Ile eaa ace ggc aca gta tgc gta ccc gaa Gin Thr Gly Thr Val Cys Val Pro Giu 250 Ceg ttt Pro Pbs 255 aaa gta teg Lys Val Sex gee caa gee Mla Gin Ala 275 tte gge age gtt Pbs Giy 5cr Val tgg. eag Trp Gin 265 atg acc age Met Tin 3cr ace atc ea Thr Ile Gin 270 ege gcc gcc Arg Mla Ala BIG. ttg ceg eec ace teg tte gee gee ate Leu Pro His Tin Sex Phe Mla Asp Ile tte cee Phe Pro 290 att ate Ile Ie 305 tge gge age ate Cys Gly ser Ile ggc gg coo aa Giy Ala Pro Lys atg agt atg eag Met Set Met Gin gee teg etc gaa gee gee geg ege gga ett tat acg ggc Giu 3cr Leu Glu Ala Giu Ala Arg Gly Leu Tyr Thr Gly ate gge tat ttg Ile Gly Tyr Leu aae ceg tgt tee ggc ggc ttg ggg ttt gaa Asn Pro Cys Set Gly Gly Leu Gly Pbs Giu 325 330 gge aug Gly Tin 335 1008 WO 01/85772 ttc aae gte Phe Asa Val att tat ca Ile Tyr Gin PCT/GB01/02003 gtt ate cgo ace ttg tog otc aeg cog ott tea gao gge Val Ile Arg Thr Leu Ser Leu Thr Pro Leu 8cr Asp Gly 340 345 350 ggc gtg tao ggt gte ggt too gge ate gto ate gao age Gly Val Tyr Gly Val Gly Ser Gly Ile Val Ile Asp Ser 1056 1104 gao cot gec gee gaa tat ogg Asp Pro Ala Ala flu Tyr Arg 370 375 gaa tgc ggc tgg Giu Cys Gly Trp aaa gee cgt ttc ctc Lys Ala Arg Phe Leu 380 1152 gaa ttg 090 Glu Leu Arg ccc gac Pro Asp 390 ttc gge att ttt Phe Gly Ile Phe ace otg gco gtg Thr Leu Arg Val 1200 aao gga cgo tge gee ctg eto gao ego Asn Gly Arg Cys Ala Leu Leu Asp Arg 405 eta tgo cgt otg Leu Cys Arg Leu aaa ace Lys Thr 415 1248 toe gee caa ser Ala Gin ate aaa eaa Ile Lys Gin 435 etc aac otg cc leu Asn Leu Pro cc gao ggt tgo Pro Asp Gly Cys gaa aat oaa Glu Asn Gin 430 cgo ate aaa Arg Ile Lys 1296 tao atc gca ego ttg ccc gao gge gcg Tyr lie Ala Arg leu Pro Asp Gly Ala 440 1344 gee etg Ala Leu 450 etc get tea leu Ala Ser gao ggo Asp Gly 455 oaa ego Gin Arg 470 ato age ctg toe Ile Ser Leu Ser gee gtt tta aao Ala Val Leu Asn 1392 1440 otg ace gao aaa Leu Thr Asp Lys gte ate att Val Ile Ile cog acc ate etg Pro Thr Ile Leu gca eaa aae tao etg ego ego tto aaa Ala Gin Asa Tyr leu Arg Arg Pie Lys 485 acc tgo ego ace Thr Cys Arg Til gte tto Val Phe 495 1488 gac caa gcg Asp Gin Ala ttt ttc aat Phe Pie Asn 515 eaa ace gee gaa Gin Tir Ala Glu eaa ggo gcg ttc Gin Gly Ala Phe gac ago etg Asp Ser Leu 510 age aae gtg Ser Asn Vai 1536 1584 toa gao ggo ate Ser Asp Gly le etc gaa ggo gge leu Giu ely Gly WO 01/85772 ttc gtc aaa cat Phe Val Lys His 530 att tta aac ggc Ile Leu Asn Gly 545 tat ttg ceaa aca Tyr Leu Gin Thr ctg caa gaa gcc Leu Gin Glu Ala 580 ttt gcc gCC gcc Phe Aa Ala Ala 595 caa tgg Gin Trp 535 cgc caa Arg Gin gta atc Val Ile atc cgc Ile Arg ctc acg coo tot Leu Thr Pro Ser 540 9CC gtg ttg gac Ala Val Leu Asp 555 gaa ace cay atc Glu Thr His Ile 570 ctc tcc aac gcc Leu Ser Asn Ala 585 PCT/GBO1/02003 gac 1632 Asp aaa 1680 Lys 560 ace 1728 Thr gte 1776 Val 1794 <210> 174 <211> 598 <212> PRT <213> Neisseria <400> 174 Leu Pro Arg Ile 1 Ser Gly Arg Ala Arg Pro Giu GlU Gin Lye Gly Len Pro Len Thr Gly Trp Phe Ala Asp His Set Asp Gly 100 meningitidis Ala Met Pro Tyr Phe 5 Lys Arg Tyr Gin Asn 25 Leu Asp Ale Leu Asp 40 His Ala Val Leu Phe Val Glu Ser Giu Arg 70 Cys Ala Asp Thr Asp Len Pro Ala Gly ile 105 339 Ala Len His Val Gly Ala Ala Asp Gy Gly Ala Ala Set Thr Asp Asp Ser Arg Gin Ser Gly Phe Len Ala Trp Leu Gin Se: 110 WO 01/85772 Ser Glu Ala 115 PCT/GB01/02003 Asp Tyr Leu Asp His Ile Arg Gin Ile His Glu Ala Ile 120 125 Arg Gin 145 Val Ala Asp Gly Lys 225 Leu Lys Ala Phe Ile 305 Ile Phe I Arg 130 Ala Pro Trp Gly Asp 210 Asn Gly Val Gin Pro 290 Ile Gly \sr Gly Tyr Tyr Thr Thr 195 Gly Arg Lys Ser Ala 275 Cys Glu Tyr Val Asp Gly Ala Leu 180 Ile Gin Ala Ile Arg 260 Leu Gly Ser Leu Val 340 Thr Asn Val 165 Cys Ser Asp Glu Ala 245 Phe Pro Ser Leu Asn 325 Ile Tyr Pro 150 Leu Phe Thr Glu Aan 230 Gin Gly His Ile Glu 310 Pro Arg Gir 135 Val Ser Ser Glu Arg 215 Val Thr Ser Thr Thr 295 Ala Cys Thr Ile SLys SHis Pro Pro 200 Arg Met Gly Val Ser 280 Gly Glu Ser Leu Asn Tyr Leu Tyr Leu Pro 170 Glu Leu 185 Met Lys Ala Ala Ile Val Thr Val 250 Trp Gn 265 Phe Ala Ala Pro Ala Arg Gly Gly 330 Ser Leu 345 Thr Gin 155 Asp Phe Gly Glu Asp 235 Cys Met ASp Lys Gly 315 Leu Thr Thr Arg 140 Arg Leu Ala Gin Leu Lys Thr Ala 205 Leu Gin 220 Leu Leu Val Pro Thr Ser Ile Leu 285 Lys Met 300 Leu Tyr Gly Phe Pro Leu Leu Arg Gly Ile 190 Pro Ala Arg Glu Thr 270 Arg Ser Thr Glu 3er 350 His Gin Gin 175 Gly Ile Asp. Asn Pro 255 Ile Ala Met Gly Gly 335 Asp Leu Pro 160 Ser Ser Leu Pro Asp 240 Phe Gin Ala Gin Ser 320 Thr Gly Ile Tyr Gin Gly Val Tyr Gly Val Gly Ser Gly Ile Val Ile Asp Ser 355 Ifn e WO 01/85772 Asp Pro Ala Ala 370 Asn Glu Leu Arg 385 Asn Gly Arg Cys Ser Ala Gln Ala 420 Ile Lys Gin Tyr 435 Ala Leu Leu Ala 450 Arg Leu Thr Asp 465 Ala Gln Asn Tyr Asp Gin Ala Trp 500 Phe Phe Asn Ser 515 Phe Val Lys His 530 Ile Leu Asn Gly 545 Tyr Leu Gln Thr Leu Gin Glu Ala 580 Phe Ala Ala Ala 595 Glu Tyr Arg Glu Cys 375 Pro Asp Phe Gly Ile 390 Ala Leu Leu Asp Arg 405 Leu Asn Leu Pro Leu 425 Ile Ala Arg Leu Pro 440 Ser Asp Gly Ile Ser 455 Lys Gln Arg Val Ile 470 Leu Arg Arg Phe Lys 485 Gln Thr Ala Glu Thr 505 Asp Gly lie Leu Leu 520 Arg Gly Gln Trp Leu 535 Ile Met Arg Gln Ala 550 Asn Gln Val Ile Glu 565 Glu Glu Ile Arg Leu 585 Leu Ala Gly Phe His 410 Pro Asp Leu Ile Thr 490 Gin Glu Thr Val Thr 570 Trp Glu 395 Leu Asp Gly Ser Ser 475 Thr Gly Gly Pro Leu 555 His Lys 380 Thr Cys Gly Ala Arg 460 Pro Cys Ala Gly Ser 540 Asp Ile Ala Leu Arg Cys Phe 445 Ala Thr Arg Phe Arg 525 Leu Glu Thr PCT/GB01/02003 Leu Glu 400 Thr Gin Lys Ann Pro 480 Phe Leu Val Asp Lys 560 Thr Ser Asn Ala Leu Arg Gly Val 590 WO 01/85772 <210> 175 <211> 279 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 175 ttg caa aac ttt cgg aaa ccc a Leu Gin Asn Phe Arg Lys Pro I 1 ggt ccg ttt tgc ccc tac tge g Gly Pro Phe Cys Pro Tyr Cys 1 gca ggt gtc gga cat ate gac g Ala Gly Val Gly His lie Asp G gcc ttt gcc gaa atg cag cag c Ala Phe Ala Glu Met Gin Gin I att ttc ate ggc gaa acg cac g Ile Phe Ile Gly Glu Thr His V ctc cag cag gaa ggc ggg ctg g Leu Gin Gin Glu Gly Gly Leu A PCT/GB01/02003 <210> 176 <211> 93 <212> PRT <213> Neisseria <400> 176 Leu Gln Asn Phe 1 Gly Pro Phe Cys Ala Gly Val Gly meningitidis Arg Lys Pro Asn Met Gin Thr Val Thr Met Tyr Thr 5 10 Pro Tyr Cys Thr Met Ala Lys Arg Leu Leu His Ala 25 His Ile Asp Glu Ile Arg Val Asp Ala Ser Pro Glu 342 WO 01/85772 PCT/GBOI/02003 40 Ala Phe Ala Glu Met Gin Gin Leu Ser Gly Gin Arg 5cr Val Pro Gin 55 Ile Phe Ile Gly Giu Tin is Val Gly Gly Phe Thr Asp Leu Tyr Arg 70 75 Leu Gin Gin Glu Gly Gly Leu Asp (fly LeU Leu Ann Pro <210> 177 <211> 2274 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(2274) <400> 177 atg aca aca tta cat ttc tca ggc ttc ccg cgt gtc ggt goc ttc cgc 48 Met Thr Tin Lou His Phe 5cr Gly Phe Pro Arg Val Gly Ala Phe Arg 1 gaa Glu caa Gin cac His ttc Phe gcc Ala tgg cgc Trp A-rg ttg cgc Lou Arg gtt 9c Val Al a gte gcc Val Ala cta tct Leu 8cr caa ctg gcg cgc ggt nac aaa gac caa ttc gct atc gaa atg acc aaa WO 01/85772 WO 0185772PCTIGBOIIO2003 Gin Leu Ala Arg Gly Asn Lys Asp Gin Pita Ala le G1u Met Thr Lys 1.05 110 ctg Lea gct Ala ttg Leu tac Tyr 225 ttc Phe gac Asp ttc Phe aac tac Asn Tyr goc aat Ala An ggc ctg Gly Leu 150 gtc ggt Vai Gly 165 cct aaa Pro Lys gcc ggt Ala Gly ctg cct Lea Pro agc aaa Ser Lys 230 gcc gaa Ala Giu 245 atc gac Ile Asp gac a Asp Lys cac tac His Tyr 120 gcc sa Ala Lye 135 as gcc Lys Ala eec gaa Lys Glu ctg ttg Lea Lea gc gag Ala G2la 200 aaa ga Lys Giu 215 gte agt Val Set cac gcc His Ala ttg gta Lea Val gtt ctg Val Leu 280 Oct gaa Pro Glu gtt cas Val Gin 140 ace gtt Thr Val 155 gcc gtt Ala Val tac gtt Tyr Val caa atc Gin Ile gsa gce (flu Ala 220 ate ctg Ile teu 235 ttg aaa Lea Lye cot gaa Pro Gia ggc gtc Gly Val cac His ctg Lea ggt Gly ttc Phe ate Ile 190 gag Giu eec Lys agc ctg Lea ctg Leu 270 gac Asp 480 672 720 sat att tgg cgc gcc aec ctg aac ass gtt ttg gas act gtt gag ctt 91 912 WO 01/85772 Asn Ile Trp Arg 290 atg oaa goc ass Leu Gin Ala Lays 305 otg otg cac act Leu Leu His Thr sac ac ccc gao Asn Lays Pro Asp 340 caa gas ttg ago. Gin Giu Leu Arg 355 gtt gao gas gas Val Ala Giu Giu 370 sac ago agc gaa Asn 3cr Ser Glu 385 ttg cot gao aac Laeu Pro Ala Asn aaa gog oaa oa Lays Ala Gin Gin 420 ato ggt tct ttc Ile Gly 5cr Phe 435 tto aaa ass ggc Phe Lays Lays Gly 450 as gas atc gc Lays Giu Ile Ala 465 Ala ctg Laeu cog Pro 325 ctg Leu gtC Val ctg Leu atc Ie ga Ala 405 gos Ala Cog Pro gas Giu ttg Leu Asn ggo Gly 310 ttt Phe tac Tyr otg Leu got Ala oa His 390 gao Asp tgg Trp caa Gin otg Laeu gtg Val 410 Lee Asn 295 gao ogt Asp Arg gao ttg Asp Laeu tot tgg 5cr Trp, asa gc Lays Ala 360 goo ago Ala 5cr 375 czgt goa Arg Ala osa ago Gin Arg ttg aso Leu An act aoo Thr Thr 440 tot gao 3cr Ala 455 gtt gaa Val Gle Lays ttg Leu tca 5cr ttg Lee 345 ga Ala oa Gin gao Asp ass Lays ctg Lee 425 gas Gie gao Ala gag Gie Val tgg Trp gtt Val 330 goa Ala ttg Leu got Ala gtt Val too 5cr 410 cot Pro ato Ile gat Asp oaa Gin Lee att Ile 315 gas Giu tte Phe aao An got Ala gao Ala 395 oca Pro atg Lcu ago Arg tac Tyr gaa ale 4.75 Gie 300 too Sex gsaa Giu a Thr gaa Gle gao Ala 380 aaa Lys ttt Phe otg Leu oa Gin gee ale 460 aaa Lays Thr ago Sex aaa Lys Ctg Leu ggo Gly 365 gao Asp Oga Arg gao Ala ocg Pro goa Ala 445 gc Ala otg Leu Val tot 5cr otg Leu oaa Gin 350 ogt Arg too 5cr otg Leu gao Asp aog Thr 430 ago Arg gcg Ala gao Asp Gie tgc Cys aaa. Lays 335 aa Lays gat Asp cgt Arg gao Al a ogt Arg 415 aco Thr ga Ala atg Met ttg Leu PCT/GBO1/02003. Lee tog 960 320 gao 1008 Ala aao 1056 Thr tot 1104 Ser gao 1152 Al a gat 1200 Asp 400 ata 1248 Ile aao 1296 An gao 1344 Ala ass 1392 Lays gao 1440 Asp 480 ttC 1488 gte otg gta aa ggo gas gao gag agt aao gac atg gtt gsa tao WO 01/85772 Val Leu Val PCT/GBOI/02003 His Gly Giu Ala Glu Arg Ann Asp Met Val Glu Tyr Phe gga Sly ago 3cr ago Set Leu 545 ctg Leu aaa Lys gca Ala ctg Leu gac 625 cac His get Ala atg 485 ttg ttg age Leu Leu 5cr 500 ggt tot ego Sly Set Arg 515 cot gaa gco Pro Glu Ala aaa ago cog Lys Ang Pro tgg tot tte Trp Set Phe 565 ate goa otg Ile Ala Leu 580 ato aaa gte Ile Lys Val 595 ttg aa agt Leu Lys Arg tto ogo etg Phe Arg Leu cat atg tgc His met Cys 645 atg gat geg Met Asp Ala 660 etc ttg ac 490 495 goa tte Ala Phe 505 aaa eca Lys Pro 520 gta get Val Ala ggt atg Gly Met aac gat Asn Asp aac gao Asn Asp 585 ate gao Ile Asp 600 tgg gat Trp Asp ace ggt Thr Gly gag tto Gin Phe ate ace Ile Thr 665 gge gaa 346 aco caa Thr Gin ceg att Pro Ile tgg tot Trp 5cr ttg ace Len Thr 555 att ect Ile Pro 570 gaa gta Gi1 u Val gaa ot Gin Pro goc tac Ala Tyr tga gaa Cys Giu 635 aaa gac Asn Asp 650 ae gag Ile Glu ttc aaa tat Tyr ato Ile act Thr 540 ggo Gly ego Atg ttg Leu gee Ala ctg Len 620 gao Asp ate Ile act Thr tac gge tgg Gly Trp 510 tto ggt Phe Sly 525 tac gca Tyr Ala cot gta Pro Val tot ace Set Tb: gat ctg Asp Lets 590 ate ege Ile Arg 605 aac tgg Asn Trp age act 5cr Thr otg ct Lets Pro tea egt Set Arg 670 ccg aac gta oaa Val Gin gaG gta Asp Val ena age Gin so: ace att Tb: Ile 560 gtg tgt Vai Cys 575 gaa aaa Gin Lye gaa gge Gin Sly Ala Sly oaa ate Gir Ile 640 gag atE Ala Ile 655 tee gao Ser Asp gac ate 1536 1584 1632 1680 1728 1776 1824 1872 1920 1568 2016 2064 WO 01/85772 Met Glu Leu 675 ggc ccg ggg Gly Pro Gly 690 gaa gtg gag Glu Val Glu 705 cgt ctg tgg Arg Leu Trp gaa act ctg Glu Thr Leu cgt gcc gaa Arg Ala Glu 755 Thr Ala tac gac Tyr Asp ctg ttg Leu Leu 710 aac ccg Asn Pro 725 caa etc Gin Leu gcg aaa Ala Lys Phe Gly Glu Phe Lys Tyr Pro 680 685 ate cac age ccg cgc gta cog Ile His Ser Pro Arg Val Pro 695 700 cgc aaa gcc ate gag gtt gta Arg Lys Ala Ile Glu Val Val 715 gac tgc ggc ctg aaa aca cgc Asp Cys Gly Leu Lys Thr Arg 730 caa gtg atg atg aac gta ace Gin Val Met Met Asn Val Thr 745 PCT/GB01/02003 Ile gcc 2112 Ala 2160 2208 2256 2274 <210> 178 <211> 758 <212> PRT <213> Neisseria <400> 178 Met Thr Thr Leu 1 Glu Leu Lys Phe Gin Glu Leu Leu His Gin Ala Ala Phe Tyr Asp His Ala Arg Phe Gly meningitidis His Phe Ser Gly Phe Ala Gin Glu Lys Tyr Ala Val Ala Lys Asp Ala Asn Ala Asp Tyr Ile Leu Asp Leu Gin Phe Asp Ser Gin Asn WO 01/85772 Gin Leu Ala PCT/GB01/02003 Arg Gly Asn Lys Asp Gin Phe Ala Ile Glu Met Thr Lys 100 110 Trp Thr Ala 145 Thr Leu Ala Leu Tyr 225 Phe Asp Phe Asn Leu 305 Leu Phe Glu 130 Gin Phe Ser Leu Thr 210 Ala Gly Gly Ala Ile 290 Gin Leu Asp Thr 115 Phe Lys Ala Leu Leu Trp Leu Leu 180 Val Glu 195 Val Asp Thr Leu Ser Val Leu His 260 Asp Tyr 275 Trp Arg Ala Lys His Thr Asn Ala Gly Val 165 Pro Ala Leu Ser Ala 245 Ile Asp Ala Leu Pro Tyr Asn Leu 150 Gly Lys Gly Pro Lys 230 GIu Asp Lys Asn Gly 310 Phe i Tyr 120 Lys Ala Glu Leu Glu 200 Glu Ser Ala Val Leu 280 Asn Arg Leu SLeu His Lys Lys Pro 185 Trp Trp Ala Ala Arg 265 Ser Lys Leu Ser Leu 345 Val Tyr Pro Gly 170 Val Ile Val Lys Leu 250 Ala Ala Val Trp Val 330 Ala Pro Val Thr 155 Ala Tyr Gin Glu lle 235 Leu Pro Gly Leu Ile 315 Glu Phe Glu Gin 140 Val Val Val Ile Ala 220 Leu Lys Glu Val Glu 300 Ser Glu Thr Phe 125 Gin Val Glu Glu Asp 205 Tyr Leu Ser Gin Ile 285 Thr Ser Lys Leu His Leu Gly Phe Ile 190 Glu Lys Ser Leu Leu 270 Asp Val Ser Leu Gin 350 Ala Gin Pro Asp 175 Leu Pro Asp Thr Pro 255 Asp Gly Glu Cys Lys 335 Lys Asp Glu Leu 160 Arg Thr Ala Val Tyr 240 Val Ala Arg Leu Ser 320 Ala Thr 325 Asn Lys Pro Asp 340 Leu Tyr Ser Trp WO 01/85772 Gin Glu Leu 355 PCT/GB01/02003 Arg Val Leu Lys Ala Ala Leu Asn Glu Gly Arg Asp Ser 360 365 Val Asn 385 Leu Lys Ile Phe Lys 465 Val Gly Ser Ser Leu 545 Leu Lys Ala Ala 370 Ser Pro Ala Gly Lys 450 Glu Leu Glu Tyr Arg 530 Thr Gin Gin Gly Glu Ser Ala Gin Sex 435 Lys Ile Val Leu Gly 515 Pro Lys Trp Ile Ile 595 Glu Glu Asn Gin 420 Phe Gly Ala His Leu 500 Ser Glu Arg Ser Ala 580 Lys Ser Gin Ala Ala Asp Val Arg Lys Ser 410 Asn Leu Pro 425 Thr Glu Ile 440 Ala Ala Asp Glu Glu Gin Glu Arg Asn 490 Ala Phe Thr 505 Lys Pro Pro 520 Val Ala Trp Gly Met Leu Asn Asp Ile 570 Asn Asp Glu 585 Ile Asp Glu 600 349 Ala Asp Ser 380 Lys Arg Leu Phe Ala Asp Leu Pro Thr 430 Gin Ala Arg 445 Glu Ala Ala 460 Lys Leu Asp Met Val Glu Tyr Gly Trp 510 Ile Phe Gly 525 Thr Tyr Ala 540 Gly Pro Val Arg Ser Thr Leu Asp Leu 590 Ala ile Arg 605 WO 01/85772 Leu Pro Leu Lys 610 Glu Ser Phe Arg 625 His Thr His Met Ala Ala Met Asp 660 Met Glu Leu Leu 675 Gly Pro Gly Val 690 Glu Val Glu His 705 Arg Leu Trp Val Glu Thr Leu Glu 740 Arg Ala Glu Leu 755 Asp Ala Tyr Leu Asn 620 Gly Cys Glu Asp Ser 635 Phe Asn Asp Ile Leu 650 Thr Ile Glu Thr Ser 665 Glu Phe Lys Tyr Pro 685 Ser Pro Arg Val Pro 700 Ala Ile Glu Val Val 715 Gly Leu Lys Thr Arg 730 Met Met Asn Val Thr 745 PCT/GB01/02003 i Gly SIle 640 Ile Asp Ile Ala Glu 720 Lys Leu Gin Val Lys His Lys Leu 750 <210> 179 <211> 867 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 179 atg aaa ccc ata cgg aaa gcc gtc ttc ccc gtc gca ggg atg gga aca Met Lys Pro Ile Arg Lys Ala Val Phe Pro Val Ala Gly Met Gly Thr 1 5 10 cgc ttc ctg ccc gcc ace aag gca age ccg aaa gaa atg ctg ccc ate Arg Phe Leu Pro Ala Thr Lys Ala Ser Pro Lys Glu Met Leu Pro Ile 350 WO 01/85772 WO 0185772PCT/GBO1/02003 gte gac Val Asp gge tgc Gly Cys gas gac Giu Asp ego cat Arg His ase att As Ile cac gee His Ala gtg att Val Ile 130 eas atg Gin Met 145 gaa act Glu Tin gsa eag Giu Gin sag coo Lys Pro sag Lys seq Thr oat His ass Lys ace Thn gtc Val 115 ttg Lou gtc Val gte Val etc Lou gas Glu 195 cog Pro gas Glu tte Pho gac Asp tge Cys 100 ttg Lou get Ala gas Giu ga Glu aa Lys IS0 gao Asp ctg Lou atg mot gac Asp ass Lys etc Lou tge Cys gac Asp gtg Val. eeg Pro 165 eag Gin geg Ala tao gee Tyr Ala 40 gtt See Val Tin tac ga Tyr Glu gsa eac Glu His qgt eag Arg Gin 105 gee gee Ala Ala 120 ate gst le Asp ego age Arg Ser acne gge Thr Gly ego att Axg Ilo 185 ase ott Asn Lou 200 gas gas gee gtg gsa gee Giu 'flu Ala Val GlU Ala ego Arg ttg Lou etg Lou gge Gly 110 ceo Pro gog Ala ttg Lou gto Val ga Giu 190 ego Arg age Sor gas GIu cog Pro ttg Lou ttt Pho etc Lou ggo Gly gaa cflu 175 a Lys tao Tyr ate Ile atg met cog Pro gga Gly gee Ala aas Lys gta Val 160 see Thr 000 Pro ate le 192 240 288 336 384 432 480 528 576 624 672 ott ace ecg ego att ttc gao tta otg ace gga ctg ceg ego ggo gog Lou Thr Pro Arg Ile Phe Asp Lou Lou 351 Thr Gly Lou Pro Arg Gly Ala WO 01/85772 210 ggc aac gaa atc Gly Asn Glu Ile 225 gaa ttt gtc cta Glu Phe Val Leu age aaa ctg ggc Ser Lys Leu Gly 260 ccc gaa ace ggc Pro GIu Thr Gly 275 gaa Glu <210> 180 <211> 289 <212> PRT <213> Neisseria <400> 180 Met Lys Pro Ile 1 Arg Phe Leu Pro Val Asp Lys Pro Gly Cys Thr Glu Glu Asp His Phe Arg His Lys Asp Asn Ile Thr Cys 100 PCT/GB01/02003 cag Gin gcg Ala 245 tat Tyr gaa Glu atc gcc Ile Ala 235 ggc acg Gly Thr 250 gtc gcc Val Ala ctt ttg Leu Leu 220 aag ctg ctc Lys Leu Leu cge tac gac Arg Tyr Asp tac gge ctg Tyr Gly Leu 270 gaa aaa tac Glu Lys Tyr 285 cac 720 His 240 ggc 768 Gly cac 816 His ace 864 Thr meningitidis Arg Lys Ala Val Phe Ala Thr Lys Ala Ser Leu Ile Gln Tyr Ala Met Val Phe Val Thr 55 Asp Lys Ala Tyr Glu 70 Lys Leu Leu Glu His Leu Tyr Ile Arg Gln 105 352 Val Ala Lys Glu Glu Glu Arg Asn Glu Thr Arg Asn Glu Ala WO 01/85772 His Ala Val Leu 115 Val Ile Leu Ala 130 Gin Met Val Glu 145 Glu Thr Val Glu Glu Gin Leu Lys 180 Lys Pro Glu Asp 195 Leu Thr Pro Arg 210 Gly Asn Glu le 225 Glu Phe Val Leu Ser Lys Leu Gly 260 Ala Ile Asp Ala Ser Gly Gly Ser 170 Ile Thr 185 Leu Ala Leu Thr Gly Ile Glu Gly 250 Thr Val 265 Gly Asp Gin Lys 140 Asn Ser 155 Tyr Gly Gly Ile Val Val Gly Leu 220 Ala Lys 235 Thr Arg Ala Tyr PCT/GB01/02003 Ala I Lys Val 160 SThr Pro Ile Ala His 240 Gly His Pro Glu Thr Gly Glu Pro Phe Arg Arg Leu Leu Glu Lys Tyr Arg Thr 275 280 285 Glu <210> 181 <211> 603 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> WO 01/85772 <400> 181 atg aca ata Met Thr Ile 1 gaa cac ace Giu His Thr tgt coo ttt Cys Pro Phe ato agc gat Ile Ser Giu gat ctg Cag Giu Leu Gin otq otg gtt Leu Leu Val atg oto gac Met Leu Asp atc ato caa Ile Ile Gin gat boa gao Asp 5cr Asp 130 ctg Cac otc Leu His Leu 145 coo gao got Pro Asp Al-a atg gga cat ato aat act too gaa aat aaa gat got gtt Ile Asn Thr 5cr (flu Asn Lys Asp Al-a Vai PCT/GBO1/02003 goc 48 Al-a atg ast Met An 5 gga caa Gly Gin goc aaa Al-a Lys goc aaa Al-a Lys oga otg Arg Leu c cg His Pro abt goc Ile Al-a 100 atc gc Ile Al-a gao atc Asp Ile ate ogo Ile Arg tog goa Ser Al-a 165 gag ggc gat gec Asp Al-a cog ttt Pro Phe ggc aac Gly An 135 gaa gac Giu Asp 150 ata ttc Ile Phe tgg goa gto Val aao Asn ttg Leu aco Thr ttC Phe gaa Gl-u tto Phe ogt Arg aaa Lys 155 ato Ile ato Ile 144 aca too tgc Thr 5cr Cys Met Gl-y His GiU Gl-y Trp Ala Lys WO 01/85772 ccg cac aat aag aaa aat att tea aaa Pro His Asn Lys Lys Asn Ile Ser Lys 195 200 PCT/GB01/02003 603 <210> 182 <211> 201 <212> PRT <213> Neisseria meningitidis <400> 182 Met 1 Glu Cys Ile Glu Leu Met lie Asp Leu 145 Pro Met Thr Ile His Thr Pro Phe Ser Glu Leu Gin Leu Val Leu Asp Ile Gin 115 Ser Asp 130 His Leu Asp Ala Gly His Met Asn 5 Gly Gln Ala Lys Ala Lys Arg Leu His Pro Ile Ala 100 Ile Ala Asp Ile Ile Arg Ser Ala 165 Glu Gly 180 Ser Glu Lys Ala Val Lys Ser Phe Pro Ala Pro Asp Val Val 105 Pro Asp Thr Asn Ile Ala Arg Asn 170 Ala Leu Ala Glu Thr Asp Gly Thr Thr Phe 160 Lys Leu Gly Ile Thr Ser Cys Pro Tyr WOI/85772 Pro His Asn Lys Lys Asn Ile 3cr Lys .195 200 <210> 183 <211> 1338 <212> DNA <213> Neisseria meningitidis <220> PCT/GBO1/02003 <221> CDS <222> (1338) <400> 183 ttg eac aec ott c Lou His Thr Lou L etg eac gaa ttc g Lou His Gin Phe G gtt gtg cgt ttt t Val Val Arg Pho S ego ggo gac ace g Arg Gly Asp Thr G gte aaa atg gte g Val Lys Met Val A ccc tao get ttt g Pro Tyr Ala Pho A gcc gcc gge ccg 0 Ala Ala Gly Pro LI 100 ctg age ttt tcc t Leu 3cr Phe 3cr P: 115 got ttt ate ttc gee atecetg att ttg gte age Ala Phe Ile Phe Ala Ile Lou Ile Lou Val cac tac ate gte gee aga ttg tge ggc gte aag His Tyr Ile Val Ala Arg Lou Cys Gly Val Lys 25 gte gge ttc ggc a ceg ttt ttc ace ega aag Val Gly Pho Gly Lys Pro Phe Pho Thr Arg Lys 40 tgg tgc etc gee cog att cog ttg ggc ggt tac Trp Cya Lou Ala Pro Ile Pro Lou Gly Gly Tyr 55 aeg ege gas gge gas gta tea gas gee gat tta Thr Arg Giu Gly Giu Val 5cr Giu Ala Asp Leu 70 75 aa esa eae ccc gee aag ege ate gee ate gte Lys Gin His Pro Ala Lys Arg Ile Ala Ile Val ace aae etc gca ctg geg gtt ttg etg tac gga Thr Ann Leu Ala Leu Ala Val Lou Lou Tyr Gly 105 110 gge gtt ace gan etg ego eec tat gte gge a Gly Val Thr Giu Lou Arg Pro Tyr Val Gip Thr 120 125 gte gaa ccc gac ace att gee gee ege gee gge tte ena age gge gac WO 01/85772 Val Giu Pro 130 aaa ata caa Lys Ile Gin 145 oaa aco gaa Gin Thr Giu gtt cag aog Val Gin Thr ggo acg cog Gly Tin Pro 195 ctg atg coo Loeu Met Pro 210 ago coo gcc Ser Pro Ala 225 geo gac ggc Ala Asp Gly ego caa ago Mrg Gin 5cr can &cc cat Gin Thr His 275 cac ace ctg His Thr Lieu 290 tgg gac gog Trp Asp Ala 305 Ile Ala 135 ano ggc Ann Gly 150 ctc aae Leu An Gly Ala ggt aa Gly Lys ate non Ile Thr 215 gca ggo Ala Gly 230 atc gee Ile Ala an aaa Lys Ly's gac ato Asp Ile ego gte AMg Val 295 cgc cgc Arg Mrg 310 Ala Mrg ace cc Thr Pro etc gan Loeu Giu ona ae Gin Thn 185 ato gen Ile Ala 200 nec gtt Thr Val otg aaa Loeu Lys ten tgg 5cr Trp ato aco Ile Thr 265 oge ccc Mrg Pro 280 ggc ctc Giy Leu agc tao 5cr Tyr Ala Gly gtt gen Vai Aln 155 gcc ggo Ala Giy 170 gtc ego Val Mrg an eac Lys An gcc ggc Ala ely cog ggc Pro Gly 235 can gaa Gin Giu 250 otg acc Loeu Thr gat act Asp Thr cgt cog Arg Pro cgt ccg Mrg Pro 315 Pbs Gin 140 gat tgg Asp Trp aaa gtc Lys Vai ace ate Thr Ile can ggc Gin Gly 205 ggo gtg Gly Val 220 gac agg Asp Arg tgg gca Trp Ala tao gaa Tyr Giu gte gan Val Glu 285 cag cog Gin Pro 300 tot gtt 5cr Val PCT/GBO1/02003 Ciy Asp ago gog 480 Ser Ala 160 gto ggc 528 Val Giy 175 gee gen .576 Ala Ala ate ggn 624 Ile Gly ana ggo 672 Lys Gly act goc 720 Thr Ala 240 ctg ace 768 Lieu Thr 255 gcc gga 816 Ala Gly coo gac 864 Pro Asp agg gcg 912 Mrg Ala ogo gee 960 Arg Ala 320 ttc ggo atg ggc tgg gaa ana acc gtt too one tog tgg aca ace oto 00 1008 WO 01/85772 Pbs Gly Met ttt ttc Lys Phe Phe toc ggt ccg Ser Gly Pro 355 ggc ttg caa Gly Lou Gin 370 ggc gtg ctg Sly Val Leu 385 gtg ttt tat Vol Phe Tyr gtc cat aac Val Gin Asn att go Ie Mla ttg gaa Lou (flu 375 ctg ccc Leu Pro 390 gaa tgg Glu Trp ttg cgc Leu Arg PCT/GB01/02003 *LeU tt 1056 Ile ctc 1104 *Leu ctc 1152 *Lou ctc 1200 Leu 400 cgc 1248 Arg otg 1296 met 1338 otg gcg gtc gcc ttc ttc aec gac gtt acc cgg ctg ctc ggt Met Ala Val Mla Phe Phe Asn Asp Val Thr Arg Leu Lou Giy 435 440 445 <210> 184 <211> 446 <212> PRT <213> Neisseria <400> i84 Leu His Tin Leu I. Leu His Giu Phe Vol Val Arg Phe Arg Giy Asp Thr meningitictis Lou Ala PeoIle Pho Ala Ile Leu Ile Leu Val 3cr 5 10 Gly His Tyr Ile Vol Mla Ary Lsu Cys Gly Vol Lys 25 Sor Val Gly Phe Gly Lys Pro Phe Phe Tin Mrg Lys 40 Giu Tzp Cys Lou Mla Pro Ile Pro Leu Gly Sly Tyr 358 WO 01/85772 Val Lys Met Pro Tyr Ala Ala Ala Gly Leu Ser Phe 115 Val Glu Pro 130 Lys Ile Gin 145 Gin Thr Glu Val Gin Thr Gly Thr Pro 195 Leu Met Pro 210 Ser Pro Ala 225 Ala Asp Gly Arg Gin Ser Gln Thr His 275 His Thr Leu 290 Trp Asp Ala PCT/GB01/02003 Val Asp Thr Arg Glu Gly Glu Val Ser Glu Ala Asp Leu iys Arg aa Val Lrg Pro ly Phe 140 Jla Asp ly Lys Ig Thr an Gin ly Gly 220 rly Asp lu Trp hr Tyr hr Val ro Gin 300 ro Ser Ile Ala Leu Leu 110 Tyr Val 125 Gin Ser Trp Gly val Ala Ile Asp 190 Gly Tyr 205 Val Glu Arg Leu Ala Asn Glu Arg 270 Glu Gin 285 Pro Asp Val Val WO 01/85772 305 Phe Gly Met Gly Trp 325 Lys Phe Phe Gly Lys 340 See Gly Pro Leu Thr 355 Gly Leu Gln Ser Tyr 370 Gly Val Leu Asn Leu 385 Val Phe Tyr Thr Ala 405 Val Gin Asn Ile Gly 420 Met Ala Val Ala Phe 435 Thr Val Ser Gly 345 Asp Ile 360 Phe Leu Val Pro Ile Arg Phe Gly 425 Asp Val 440 Ser His Set 330 Asn Ala Ser Ala Gly Gin Ala Leu Val 380 Val Leu Asp 395 Gly Lys Pro 410 Leu Ala Leu Thr Arg Leu PCT/GB01/02003 320 Leu Ile Leu Leu Leu 400 Arg Met <210> 185 <211> 1476 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1476) <400> 185 atg aaa tac aaa gac ctg cgc gac ttc ate gcc atg ctc gag cag cag Met Lys Tyr Lys Asp Leu Arg Asp Phe Ile Ala Met Leu Glu Gin Gin 1 5 10 ggc aaa ctc aaa cgc atc gcg cac ccc gtt tec ccg cat ttg gaa atg Gly Lys Leu Lys Arg Ile Ala His Pro Val Ser Pro His Leu Glu Met 25 ace gaa atc gcc gac cgc gtg ctg cgc gcc gaa ggg ccg gcg ttg ttg Thr Glu Ile Ala Asp Arg Val Leu Arg Ala Glu Gly Pro Ala Leu Leu 360 WO 01/85772 ttt gsa cac cos Phe Giu His Pro ttg gos sac otg Lou Ala Asn Leu gcg gac ago gtg Ala Asp 5cr Val ttg aa gaa coo Lau Lys Giu Pro 100 ctg cog oto ttg Lou Pro Leu Lou 115 sat gcg cog tgt Asn Ala Pro Cys 130 tat cas ott cog Tyr Gin Lou Pro 145 gta ag tgg ggo Val Thr Trp Gly sat oto ggc att Asn Lou Gly Ile 180 atg 090 tgg ctg Met Arg Trp Lou 195 ogo ass co aat Arg Lys Leu Asn 210 tgc gao oct gcc PCTIGBOIIO2003 ago tat Arg Tyr gtg gog Val Ala 75 999 cag Gly Gin ass gac Lys Asp gog ccg Ala Pro ggc ga Gly.Giu 140 gas gao Giu Asp 155 cog cac Pro His ggc ats Gly Ile otg gat Lou Asp gtc gcc Val Ala 220 sog coo gat Asp stg Met acg Thr gog Ala sac Aen 125 gao Asp gtt Val ass Lys sac Asn tat Tyr 205 gto Val gtt tat coo gtg Tyr Pro Val ggc atg ggc Gly Met Gly otg gog tat Leu Ala Tyr ttt too as Phe Bar Lys 110 gtg gtg ass Val Val Lye gtt gat ttg Val Asp Lou gog cog otg Ala Pro Lou 160 555 090 0Sa Lys Arg Gin 175 sag otg att Lye Lu Ile 190 cag gas ttc Gin Giu Phe gtg oto ggc Vai LaU Giy 000 oat cc 192 240 288 336 384 432 480 528 576 624 672 720 Asp Pro Ala Thr Ile Lou Gly Ala Val Thr Pro Val Pro Asp Thr WO 01/85772 PCT/GBOS/02003 ttg ago gaa tac Leu Ser Giu Tyr ttt gcc gga ctg Phe Ala Gly Leu cgc ggt tag cgg Arg Gly Ser Arg acg gaa Thr Glu 255 ctg gtg aaa Leu Val Lys atc gtg ttg Ile Val Leu 275 ato ggc aac gat Ile Gly Asn Asp caa gtg cct Gin Val Pro gao age gca gas Ala Arg Ala Glu 270 gas ggc gto atc cat cca aac gaa acc gog ttg gaa ggt Glu Gly Val Ile His Pro Asn Giu Thr Ala Leu Giu Gly cca tac Pro Tyr 290 ggc gao cac acg Gly Asp His Thr tat tao sac gag cag gao cat ttc ccc Tyr Tyr Ass Glu Gin Asp His Phe Pro 300 ttt acg gtc gas Phe Thr Val Glu ate aco atg cgc Ile Thr Met Arg aao cog att tac Asn Pro Ile Tyr 960 1008 tct ace tac ac Ser Thr Tyr Thr aaa ceg coo gat Lys Pro Pro Asp ccc gco gtt ttg Pro Ala Val Len ggo gtg ely Val 335 gcg ttg sac gas gtg tto gta cog Ala Lou an Giu Val Pie Val Pro 340 ttg cas sag cag Len Gin Lys Gin tto c0 gaa Phe Pro Glu 350 cgc atg gcg Arg Met Ala 1056 ato acc gat Ile Thr Ap 355 ttt tac ctg cog Phe Tyr Lou Pro gas ggc tgc too Glu Gly Cys Ser 1104 gtg gtg Val Val 370 ago atg aaa aaa Ser Met Lys Lys tao gcc gga cac Tyr Ala Gly His sag cgc gtg atg Lys Arg Val Met 1152 1200 ggc tgo tgg tog Gly Cys Trp Ser ctg cgo cag ttt Len Arg Gin Phe tao acc aaa ttc Tyr Thr Lys Phe att gtg gtg gat gao gat gtg get gtg Ile Val Val.Asp Asp Asp Val Asp Val 405 gao tgg asa gaa Asp Trp Lys flu gtc ato Val Ile 415 1248 1296 tgg gog gta acc acg cgo atg gac ccc gtg cgo gat ace gtt ttg atg Trp Ala Val Thr Thr Arg Met Asp Pro Val Arg Asp Thr Val Leu Met WO 01/85772 PCT/GB01/02003 gaa aac Glu Asn ctt ggc Leu Gly 450 acc gac Thr Asp 465 get aag Ala Lys 420 cc ate Pro Ile aaa atg Lys Met gaa tgg Glu Trp gat gag Asp Glu 485 ttc gcc age ccc Phe Ala Set Pro 445 acc aac aag tgg Thr Asn Lys Trp 460 aaa aaa gac cct Lys Lys Asp Pro 475 ttg ggg ttg Leu Gly Leu 490 gga Gly gaa Glu acg Thr 480 1344 1392 1440 1476 <210> 186 <211> 492 <212> PRT <213> Neiaseria <400> 186 Met Lys Tyr Lys 1 Gly Lys Leu Lys Thr Glu Ile Ala Phe Glu His Pro Leu Ala Asn Leu Ala Asp Ser Val Leu Lys Glu Pro 100 Leu Pro Leu Leu 115 meningitidis Asp Leu Arg Asp 5 Arg Ile Ala His Asp Arg Val Leu 40 Val Lys Pro Asp I 55 Phe Gly Thr Pro i Ser Lys Leu Arg I Glu Pro Pro Lys i Lys Asp Ile Trp 120 363 Met Leu Pro His Gly Pro Tyr Asp Ala Met Gln Thr Asp Ala Pro Asn 125 Glu Gin Leu Glu Ala Leu Tyr Pro Gly Met Leu Ala Phe Ser 110 Val Val WO 01/85772 Asn Ala Pro 130 Tyr Gin Lou 145 Val Tin Trp Ann *Leu (fly Met Arg Trp 195 Arg Lys Lou 210 Cys Asp Pro 225 Lou 3cr Giu Lou Val Lys Ile Val Lou 275 Pro Tyr Gly 290 Val Phe Thr 305 Thr Tyr Ala Leu An Ile Thr Asp 355 Val Val Sex Gly Giu 140 Giu Asp 155 Pro His Giy Ile Leu Asp Val Ala 220 Thr Pro 235 Arg Giy Val Pro Gin Thr Giu Gin 300 Giu An 315 Pro Ala Gin Lys Cys Set His Ala PCT/GBO1/02003 Leu Lou 160 rGin L le LPhe L Gly Thr 240 Giu LGiu t Giy Pro His 320 Val Glu Ala Met WO 01185772 Met Gly Cys 385 Ile Val Val Trp Ala Val Glu Asn Thr 435 Leu Gly Gly 450 Thr Asp Arg 465 Ala Lys Ile PCT/GB01/02003 Ser Phe Leu Arg Gln 390 Asp Asp Val Asp Val 405 Thr Arg Met Asp Pro 425 Ile Asp Tyr Leu Asp 440 Met Gly Leu Asp Ala 455 Trp Gly Arg Val Ile 470 Glu lle Trp Glu Glu 485 <210> 187 <211> 1191 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1191) <400> 187 atg ttc tte aag cac ate gaa gcc gcc Met Phe Phe Lys His Ile Glu Ala Ala 1 ttg ggc gaa gca ttc aaa gcc gaa ace Leu Gly Glu Ala Phe Lys Ala Glu Thr gge ate ggc gtg tac aaa gac gca tec Gly Ile Gly Val Tyr Lys Asp Ala Ser gcc gtc aaa gaa gcc gaa aaa cge ctg Ala Val Lys Glu Ala Glu Lys Arg Leu 365 WO 01/85772 aao tao ctc Asn Tyr Leu att otg otg Ile Leu Len aaa aca gcg Lye Thr Ala gag ttt gc Gin Phe Ala 115 cog act tgg Pro Thr Trp 130 gac oaa cot Asp Gin Pro 145 gac ggt atg Asp Gly Met otg otg 080 Len Len His gat caa tgg Gin Gin Trp 195 ccg otg ttt Pro Len Phe 210 gat goc tao Asp Ala Tyr 225 att goc age PCT/GBO1/02003 gao ggc Asp Gly aaa gac Lys Asp ott ggc Len Gly cag ttg Gin Leu 080 aao His Asn 135 tao tat Tyr Tyr 150 gao ttg Asp Leu tgo cac dye His otg goa Len Ala gco tao Ala Tyr 215 ogo gtg Arg Val 230 too aaa goc gao Ala Asp gaa ato Gin Ile ada ggo Thr Gly 105 gog oaa Ala Gin ato gc Ile Ala goo goo Ala Ala 088 gog Gin Ala 170 cot acc Pro Thr 185 ott tot Leu 8cr ggo ttc Sly Phe ttg 888 Leu Lys tto ggt atg tao aac gag ogo gto Ile Ala Set 5cr Tyr 5cr Lys Asn Phe Gly Met Tyr Asn Giu Arg Val WO 01/85772 PCT/GB01/02003 ggc gcg ttc Gly Ala Phe cac ago caa His Set Gln 275 act ttg gtg Thr Leu Val 260 gcc gaa gat gaa Ala Glu Asp Glu 265 gca ace gac Ala Thr Ala gco cgc geo Ala Arg Ala 270 aac cct get Asn Pro Ala gtc aaa acc att atc cgt acc ttg tat Val Lys Thr Ile Ile Arg Thr Leu Tyr tca cac Ser His 290 ggc gcg aac act att gcg ctg gtg Gly Ala An Thr Ile Ala Leu Val ttg aaa Leu Lys 300 aat gat gat ttg Asn Asp Asp Leu gca caa tgg att Ala Gln Trp Ile gaa etc gat gaa atg cgc ggc cgt ate Glu Leu Asp Glu Met Arg Gly Arg Ile gcc atg cgc caa Ala Met Arg Gin ttt gtc gag ttg Phe Val Glu Leu aaa gao aaa ggt Lys Ala Lys Gly gca act Ala Thr 335 1008 caa gac ttt Gin Asp Phe ttc att atc gaa caa aac ggc atg ttc Phe Ile Ile Glu Gln Asn Gly Met Phe tec ttc age ser Phe Ser 350 ttt gce att Phe Ala Ile 1056 ggc ttg act cc gaa Gly Leu Thr Pro Glu 355 caa gtc gac cgt Gln Val Asp Arg tta aaa aac Leu Lys Asn 1104 tat gao Tyr Ala 370 gtc cgc tcc ggc Val Arg Set Gly atc aac gtc gcc Ile Asn Val Ala att acc gac gac Ile Thr Asp Asp 1152 ate gat tat ctg Ile Asp Tyr Leu gaa agt atc gta Glu Ser Ile Val aaa gta ctg Lys Val Leu 395 1191 <210> 188 <211> 397 <212> PRT <213> Neisseria meningitidis <400> 188 Met Phe Phe Lys His Ile Glu Ala Ala Pro Ala Asp Pro Ile Leu Gly WO 01/85772 Leu Gly Glu Ala Gly Ile Gly Val Ala Val Lys Glu Asn Tyr Leu Thr Ile Leu Leu Phe Lys Thr Ala Gin 100 Glu Phe Ala Lys 115 Pro Thr Trp Pro 130 Asp Gin Pro Tyr 145 Asp Gly Met lle Leu Leu His Gly 180 Glu Gln Trp Glu 195 Pro Leu Phe Asp 210 Asp Ala Tyr Gly 225 Ile Ala Ser Ser Gly Ala Phe Thr 260 Lys Ala Glu Thr Lys Asp Ala Ser 40 Glu Lys Arg Leu 55 Asp Gly Val Ala 70 Lys Asp His Glu Leu Gly Gly Thr 105 Gin Leu Asn Ala 120 His Asn Ala Ile 135 Tyr Tyr Asp Ala 150 Asp Leu Ser Gin Cys His Asn Pro 185 Leu Ala Lys Leu 200 Ala Tyr Gin Gly 215 Arg Val Phe Leu 230 Ser Lys Asn Phe Val Ala GlI Asp 265 Glu Lys Thr Pro Ser Glu Asn Glu Ala Ser Leu Arg Ile Trp 125 Ala Val 140 His Gly Lys Gly Ile Asp Glu Lys 205 Asn Gly 220 Asn Thr Tyr Asn Thr Ala PCT/GB01/02003 Asn Leu Val Lys Thr Lys Thr Gin Arg Ala Ala Ala Set Asn Ile Gin Asp Trp 160 Ile Val 175 Thr Pro Trp Leu Glu Glu Leu Leu 240 Arg Val 255 Arg Ala WO 01/85772 His Ser Gin Val 275 Ser His Gly Ala 290 Lys Ala Gin Trp 305 Ala Met Arg Gin Gin Asp Phe Asp 340 Gly Leu Thr Pro 355 Tyr Ala Val Arg 370 Asn Ile Asp Tyr 385 Thr Ile Thr Ile 295 Ala Glu 310 Phe Val Ile Ile Gin Val Gly Arg 375 Cys Glu 390 Ile Arg 280 Ala Leu Leu Asp Glu Leu Glu Gln 345 Asp Arg 360 Ile Asn Ser Ile PCT/GB01/02003 SAla SLeu SLys 320 L Thr SSer I Ile SAsp <210> 189 <211> 462 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 189 ttg ctt tgc ccg gaa aaa atg Leu Leu Cys Pro Glu Lys Met 1 5 ggc gtc egg tcg ggt ttg egg Gly Val Arg Ser Gly Leu Arg 2 cat ttt aga atg acc cgt ttt His Phe Arg Met Thr Arg Phe tcg ggg atg gcg gga cag tat ccg tac 3er Gly Met Ala Gly Gin Tyr Pro Tyr 10 agg aac ggc ttg aaa ctt tgg gat att \rg Asn Gly Leu Lys Leu Trp Asp Ile 25 ate gtc gca aga tgc ggt tta ttg ttt Ile Val Ala Arg Cys Gly Leu Leu Phe 40 369 WO 01/85772 gca ace ctt Ala Thr Leu atg ctg ttc Met Leu Phe get tcg cag Ala Ser Gin gcg aag gcc Ala Lys Ala gta gac get Val Asp Asp 115 aag ctg aag Lys Leu Lys 130 gga aaa Gly Lys tgc gcc Cys Ala 70 gag ctg Glu Leu gcg gaa Ala Glu acc aag Thr Lys cag gct Gin Ala PCT/GB01/02003 tgt 192 Cys gcg 240 Ala aag 288 Lys tot 336 Ser gcg 384 Ala ccg 432 Pro gca aaa ccg gct ctg ccc gca got aaa aaa Ala Lys Pro Ala Leu Pro 145 150 <210> 190 <211> 154 <212> PRT <213> Neisseria meningit <400> 190 Leu Leu Cys Pro Glu Lys 1 5 Gly Val Arg Ser Gly Leu His Phe Arg Met Thr Arg Ala Thr Leu Lys Gly Lys Met Leu Phe Ser Cys Ala Ala Ala Lys Lys tidis Met Ser Gly Met Arg Arg Asn Gly Phe lie Val Ala 40 Thr Met Lys Lys 55 Phe Set Leu Ala 370 Gly Gln Lys Leu Cys Gly Phe Val Val Asn WO 01/85772 Ala Ser Gin Gln Ala Lys Ala Ile 100 Val Asp Asp Leu 115 Lys Leu Lys Asp 130 Ala Lys Pro Ala 145 PCT/GB01/02003 75 Gly Ile Gly Pro Ala Lys Asn Gly Ala Phe Lys Ser 110 Gly Pro Ala Val Leu Ala 125 Pro Ala Pro Lys Gly Pro 140 <210> 191 <211> 684 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 191 atg ttt got ttt tta gaa gcc ttt 1 Met Phe Ala Phe Leu Glu Ala Phe 1 5 gtt ttt ttt gta ttg gtc atc tgc Val Phe Phe Val Leu Val Ile Cys gat ttg ace ttg gta aca ggc ggc Asp Leu Thr Leu Val Thr Gly Gly 1 40 aat ccg cat att atg ttt gca gtc Asn Pro His Ile Met Phe Ala Val 55 gac ggc ate atg ttc gcc gcc gga Asp Gly Ile Met Phe Ala Ala Gly 2 70 371 gaa tac ggt Glu Tyr Gly ggc gtg ccg Gly Val Pro tog ggt atg Ser Gly Met etc ggc gta Leu Gly Val tgg ggg cag Trp Gly Gin tat gcg Tyr Ala att ccc Ile Pro ggt tat Gly Tyr ttg gtc Leu Val aaa atc Lys Ile WO 01/85772 WO 0185772PCT/GB01102003 agg Etc aaa cot att gag ago atc atg ag ccg ass cgt tat Arg Phe Lys Pro Ile Mla Arg Ile Met Tin Pro Lys Arg Tyr gag cag Giu Gin gtt cag gas as Etc ga aaa tac ggt sac tgg gto tta Val Gin Giu Lys Phe Asp Lys Tyr Gly PAn Trp Val Leu 100 105 cgt Etocatg coo ggt ttg aga ag gco gta ttt gtt aos Arg Phe Leu Pro Gly Leu Arg Thr Ala Val Phe Val Thr ttt gta gc Phe Val Mla 110 gao ggt ato Ala Gly le ago ogo Arg 130 sag gtt tos tac Lys Val Ser Tyr cgt ttt sa att Arg Phe Ile Ile gat gga ctg gc Asp Gly Len Mla gos otg att too gto Ala Le Ile 8cr Val 145 oso aac atc gat tgg His Asn Ile Asp Trp 165 atE tgg att tat Ile Trp Ile Tyr ggo gas tao ggt Gly Qiu Tyr Gly otg atg gcg as Leu Met Als Lys cac ago atg as His Ser Lou Gin tag ggt 8cr Gly 175 stt tEE gtt ato ttg ggt ats ggt Ile Phe Val Ile Len Gly Ile Gly 180 aco gtt gta got Thz Val Val Mla Egg att tgg Trp Ile Trp 190 ttg ass gas Len Lys Glu tgg ass ass Trp Lys Lye 195 ago as cgt ato Arg Gin Arg Ile ttt tao ogo Phe Tyr Arg aga ass Ser Lys 205 sag agg Lys Arg 210 gag oaa oga ass Als Gin Arg Lys goc aag goa goa Mla Lye Mla Mla aaa esa gao gag as Lys Lys 220 Msa Ala Gin ago ass oaa taa 8cr Lys Gin 225 <210> <211> <212> <213> 192 227 PRT Neisseris meningitidis <400> 192 WO 01/85772 Met Phe Ala 1 val Phe Phe Asp Leu Thr Asn Pro His Asp Gly Ile Arg Phe Lys Val Gin Glu Arg Phe Leu 115 Ser Arg Lys 130 Ala Leu Ile 145 His Asn Ile Ile Phe Val Trp Lys Lys 195 Lys Arg Ala 210 Ser Lys Gin 225 Leu Glu Leu Val Val Thr Met Phe Phe Ala 70 Ile Ala Phe Asp Gly Leu Ser Tyr Val Pro 150 Trp Leu 165 Leu Gly Gin Arg PCT/GB01/02003 Ala Glu Thr Gly Leu Gin Ala Ile Ala Ala 160 Gly Trp Glu Arg Lys Ala Ala Lys Ala Ala Lys Lys Ala Ala Gin <210> 193 WO 01/85772 <211> 1089 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1089) PCT/GBOI/02003 <400> 193 atg aat gac Met Asn Asp I acc ctt ttt Thr Len Phe cog gao aaa Pro Asp Lys ggc ggg gaa Gly Gly Gin ggc gca ago Gly Ala 5cr gtc aao ogo Val Asn Arg gaa oto aan Gin Len Lys ggg gaa atg Giy Gin Met 115 tgg atg cag Trp Met Gin 130 att ggg cgg Ile Gly Arg acg cag cag ctt caa ggt aan aan gao tao ott aan Thr Gin Gin Leu Gin Gly Lys Lys Asp Tyr Lou Lys ttg qat Len Asp ogt atq Arg Met tat gco Tyr Ala 55 ogc tgc Arg Cys coo gag Pro Gin tgg tat Trp Tyr acc atg Thr Met caa gcg Gin Ala 135 gga cag Gly Gin gaa gtg Gin Val ogt att Axrg Ile ggq cag Gly Gin goa got Ala Ala goc gog Ala Ala otg tc Len Ser agg ott Arg Len 125 ttg gat Len Asp 140 ata gto tta aaa cag gac tat gta Ile Val Len Lys Gin Asp Tyr Val 155 160 WO 01/85772 WO 0185772PCTIGBO1/02003 acg gaa act ttg Thr Giu Thr Lieu gte ggc ase cgg Val Gly Asn Arg ttc egt tat Phe Arg Tyr cgg caa att Arg Gin Ile 175 aaa atg ott Lys Met Lieu 190 gaa gge agt Glu Gly Ser gaa tgg gog (flu Trp Ala 195 ace eaa ccg aat Tin Gin Pro Asm gec gtg tgt cag Ala Val Cys Gin tgrz cgc aeg geg Cys Arg Tin Ala ggo ttg gge agc gac etg etc gas Gly Lieu Giy Sex Asp Lieu Lieu Giu 205 etg tat Lieu Tyr 210 tgc gga sac ggc Cys Gly Asn Giy aat. tte ace etg Asn Phe Thr Lieu ceg etg Pro Lieu 220 tee ega tat ttc 3cr Arg Tyr Phe eag gtt ttg gca ace gaa ate tee aaa Gin Val Lieu Ala Tr Giu Ile Ser Lys 230 teaC gte age gog- Sex Val Ser Ala eaa tgg sat ate gas Gin Trp Asn Ile Glu gee s ac ege Ala Asn Arg ate gge Ile Gly 250 sac ate ass ate An Ile Ly8 Ile gee egt Ala Arg 255 etg tet get Lieu Her Ala aaa ego ott Lys Arg Lieu 275 gas ttt ace gas Giu Phe Thr Giu tat ace gga ass Tyr Thr Giy Lys cgt gag ttt Arg Giu Phe 270 gea ttt tee Ala Phe Ser aag gat gge ggt Lys Asp Gly Gly get ttg aeg gat Ala Leu Thr Asp act att Ti Ile 290 tte gte gat ceg Phe Vai Asp Pro egt gee ggt ate Arg Ala Gly Ile gag gas act ttg Glu Giu Tin Lieu etg gtg teg cag Lieu Val Her Gin gac aae ate ate Asp As Ile Ile att tee tge ast Ile Sex Cys Asn 912 960 1008 gaa aeg ttg ego Giu Tin Leu Arg sat ttg gac ace Asn lieu Asp Thr geg gaa ace eat Ala Glu Thr His geg gte Ala Val 335 gaa egt gee Giu Arg Ala gee Ctg ttc Ala Leu Phe 340 gac esa ttc ceg ttt Asp Gin Phe Pro Phe 345 aeg cac cat ate gaa Thr His His Ile Glu 350 1056 WO 01/85772 agt ggt gta ttg ttg aaa aag aaa ate ctt tga Ser Gly Val Leu Leu Lys Lys Lye Ile Leu 355 360 PCT/GB01/02003 1089 <210> 194 <211> 362 <212> PRT <213> Neisseria meningitidis <400> 194 Met Asn A 1 Thr Leu P1 Pro Asp L Gly Gly G: Gly Ala Si Val Asn A Glu Leu L Gly Glu ME 1: Trp Met GJ 130 Ile Gly AJ 145 Thr Glu TI Glu Gly SE Leu Gin Gly Lys Lys 10 Val Pro Glu Trp Glu Arg Ala Glu Phe Arg Met Phe Glu Lys Gly Asp Arg Phe Glu Ala Leu Ile Ala Ala Ala Ala Val Glu Phe Leu 105 Ile Tyr His Lys Arg 120 Leu Gin Gln Gin Leu 140 Lys Ile Val Leu Lys 155 Asn Arg Asp Phe Arg 170 Asn Ala Ala Val Cys Asp Tyr Leu Val Tyr Glu lie Trp His Gin Lys Ala Ala Ser Glu Ala Gin Ser Ser Thr Leu 110 Leu Asp Ala 125 Asp Ile Ser Gin Asp Tyr Tyr Arg Gin 175 Gln Lys Met Leu 190 WO 01/85772 PCT/GB01/02003 Glu Trp Ala Cys Arg Thr Ala Glu Gly Leu Gly Ser Asp Leu Leu Glu 195 200 205 Leu Tyr Cys Gly Asn Gly Asn Phe Thr Leu Pro Leu Ser Arg Tyr Phe 210 215 220 Arg Gin Val Leu Ala Thr Glu Ile Ser Lys Thr Set Val Ser Ala Ala 225 230 235 240 Gin Trp Asn Ile Glu Ala Asn Arg Ile Gly Asn Ile Lys Ile Ala Arg 245 250 255 Leu Ser Ala Glu Glu Phe Thr Glu Ala Tyr Thr Gly Lys Arg Glu Phe 260 265 270 Lys Arg Leu Lys Asp Gly Gly Ile Ala Leu Thr Asp Tyr Ala Phe Ser 275 280 285 Thr Ile Phe Val Asp Pro Pro Arg Ala Gly Ile Asp Glu Glu Thr Leu 290 295 300 Lys Leu Val Ser Gin Phe Asp Asn Ile Ile Tyr Ile Ser Cys Asn Pro 305 310 315 320 Glu Thr Leu Arg Ala Asn Leu Asp Thr Leu Ala Glu Thr His Ala Val 325 330 335 Glu Arg Ala Ala Leu Phe Asp Gin Phe Pro Phe Thr His His Ile Glu 340 345 350 Ser Gly Val Leu Leu Lys Lys Lys Ile Leu 355 360 <210> 195 <211> 2145 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(2145) <400> 195 atg aat ace cca ttg ttc cgt ctc age ctg ctc tcg ctt ace ctg gcg 48 Met Asn Thr Pro Leu Phe Arg Leu Ser Leu Leu Ser Leu Thr Leu Ala 1 5 10 377 WO 0 1/85772 PCT/GBO1/02003 gat 96 gca ggt Ale Gly aec gtt Tbr Val ae gtt Ile Val gee cto Giu Leu acg tcc Thr set ae aag Ile Lys ggc age Gly Mrg eea ggo Lye Gly 130 ate ate Ile Ile 145 aaa aac Lye Asn gta agc Val Set ttg ttc Leu Phe ttt gc Phe Ala eco gta Thr Vel cg ctg Thr Leu. tta aaa Len Lys cae ttc Gin Phe gtg gac Val Asp 100 ttt att Phe Ile 115 gog ggt Ala Gly ace aaa Thr Lys tgg ggc Trp Gly tao ggo Tyr Gly 180 tot tao 5cr Tyr cat gog gea gee His Ala Ala Gin aa ggc gao ego Lye Gly Asp Arg 40 caa ca aea gao Gin Gin Lye Asp gee gag ccc tcc Giu Gin Pro 3cr ctg cg otg o Leu Thr Len Arg eec goc tat too Asn Ala Tyr 5cr gtc get coo got Val Asp Pro Ala 120 too gcc tct gee 3cr Ala 5cr Ala 135 eec gte gat gcc Thr Val Asp Ala 150 gtg ogc oto aec Val Arg Leu Asn 165 goa ago gte tte Ala 5cr Val Phe aac ogo aec aet Asn Mrg Asn Aen 200 gte Val ate Ile aco Thr gge Gly aac Asn Ott Lou gtt Val 125 cc Thr aaa Lye ago aac Asn gee gaa ae get tao Gin Lys Asp Tyr Gin Ala Gly Lys 205 WO 01/85772 ggc ttc cgt aat PCT/GB01102003 ast ttc aac ggc ggc aaa acc gta ccg tac agc gcg 672 Gly Phe Arg Awn Asn Phe Awn Gly Gly Lys Thr Val Pro Tyr 5cr Ala aaa cgc Lys Arg cac cgc His Arg acc gtc Thr Val 260 atg gaa Met Glu 275 ant ttg Asn Leu gcc aac Ala Awn ggc aco Sly Thr acc act Tin Thr 340 acc ctg Thr Leu 355 caa gcg Sin Ala act gat Thr Asp 220 aca agc Thr gac cag Asp Gin ggc gat Sly Asp gaa acc Glu Thx 285 ggc ttt Sly Phe 300 cgc tat Arg Tyr ggc ccc Gly Pro gac agc Asp Sex cgc cat Arg His 365 att. gaa Ile Glu 380 gaa aat Glu Asn 768 816 960 1008 1056 1104 1152 1200 390 WO 01/85772 WO 0185772PCT/GBOI/02003 goc ass gcc tao Ala Lye Mla Tyr tat ate gas go Tyr Ile Giu Ala 420 otg ac sac cog Leu Tin Asn Pro ac ass ac gat ace Thr Lye Tin Asp Tin 410 ggc gog Gly Ala 415 1248 att cac gag att Ile His Giu Ile ggc: ttt aco ctg Gly Phe Thr Lou ac ggc ggg Thr Gly Gly 430 ass ac gtt Lys Tin Val 1296 1344 otg ogh tao Lou Arg Tyr 435 ta ago aac Set Asn 450 gac ogo tto Asp Arg Pho sag gtg Lye Val 440 aaa ac cat gao Lye Thr His Asp sac ott sac cog agt ttc ggo gtg Asn Lou Asn Pro sex Phe Gly Val 455 tgg, cag ccg cac Trp Gin Pro His 1392 cac tgg ago ttc His Trp 5cr PMe gog ago cac at Mla 8cr His Asn goo ago ogo ago Mla 5cr Arg Sex 1440 1488 ego ctg tat gao Arg Lou Tyr Asp otg cas aoo cao Leu Gin Thr His ass cgo ggo ate Lye Arg sly Ile ate tcg Ile Sex 495 att goc ga Ile Ala Asp ttc sac tao Ftc Asn Tyr 515 ggc Gly 500 sog ass goo gas Tin Lys Ala Giu gog ogo sat sc Mla Arg Asn Tin sac gac ggo aog Asn Asp G1y Thr ttt goo Phe Ala 520 gos at ggc Als Asn Gly gsa sto ggc Giu Ile Gly 510 tac the tgg Tyr Ptc Tip cat gao tot His Asp Sex 1536 1584 cag ac Gin Tin 530 ate ass gao gcg Ile Lys Asp Ala goo sat cog Ala Asn Pro cas at ego Gin Ass Arg 540 1632 goc gtc cgt gas Ala Val Arg Giu gto sat goc ggt tao ate ass at oat Val Aen Ala Gly Tyr. lie Lye Asn His 555 1680 tao gas ttg Tyr Giu Lou ggo gta ago Gly Val Sex ggc gog Gly Ala 565 oso ago His Ser 580 too tao ogo ac Sex Tyr Arg Tin a cog ogo ttt Lye Pro Arg Phe 585 ggo otg act goo Gly Lou Thi Ala ass gtc Lys Val 575 1728 1776 tao gat sog cac ass gao sag Tyr Asp Thr His Lye Asp Lys 590 WO 01/85772 PCT/GBOI/02003 ctg ttg age geg act ect gaa. ttt ggc gca caa gtc gge ege act tgg 1824 Lou Lou 3cr Ala Asn Pro Giu Phe Gly Ala Gin Val Gly Arg Tbr Txp 595 1600 605 acg get toc ett gec tac age ttc can ac ceg aat ctg gac ate ggc 1872 Thr Ala 5cr Lou Ala Tyr Arg Pita Gin Asn Pro Asn Lou Giu Ile Gly 610 615 620 tgg age gg cgt tat gtt caan ccc gee gtg ggt teg ata ttg gtg gee 1920 Tr Mrg Gly Mrg Tyr Val Gin Lys Ala Val Gly 8cr Ile Lou Val Ala 625 630 635 640 ggt eaa aca gac ege ac ggc ccc ttg gac aac gtt gta ega aaa ggt 1968 Gly Gin Lys Asp Mrg Asn Gly Lys Lou Glu Ann Val Val Mrg Lys Gly 645 650 655 tte ggt gtg aac get *gta-ttc--gee--aac tgg ccc Gag ctg ggc ace gac 2016 Phe Gly Val Ann Asp ValiPhe Ala Ann Trp Lys Pro Lou Gly Lys Asp 660 665 670 cg ate cat gtt cat ctt tcg gtt aec ac gtg ttc aac cg ttc tac 2064 Tin Lou Asn Val Ann Lou 5cr Val Ann Ann Val Phe Asn Thr Phe Tyr 675 680 685 tat ccg cac agc ccc ega tgg ace cat ace ctg eag ggc gtg gga. egt 2112 Tyr Pro His 3cr Gin Mrg Trp Thr Ann Thr Lou Pro Giy Val Gly Mrg 690 695 700 gat gta ege ttg ggc gtg ac tac acg tte tac 2145 Asp Val Mrg Lou Gly Val Ann Tyr Lys Phe 705 710 715 <210> 196 <211> 714 <212> PRT <213> Neisseria. meningitidis <400> 196 Met Ann Thr Pro Leu Phe Mrg Lou 5cr Lou Lou 3cr Leu Thr Lou Ala 1 5 10 Ala Gly Pec Ala His Ala Ala Glu Ann Ann Ala Lys Vel Val Lou Asp 25 Thr Val Thr Val Lys Gly Asp Mrg Gin Gly 5cr Lys Ile Mrg Thr An 40 381 WO 01/85772 Ile Val Thr Glu Leu Leu Thr Ser Gin Ile Lys Val Gly Arg Phe 115 Lys Gly Ala 130 Ile Ile Thr 145 Lys Asn Trp Val Ser Tyr Leu Phe Ser 195 Gly Phe Arg 210 Leu Asp Lys 225 Gly Asp His Ile Arg Thr Ile Ser Met 275 Asn Thr Asn Gln Gin Glu Glu 70 Leu Thr Asn Ala Val Asp Ser Ala Thr Val 150 Val Arg 165 Ala Ser Asn Arg Asn Phe Ser Tyr 230 Ile Val 245 Arg Glu Arg Gin Ala Tyr Glu Ser Ile Asp Gly Met 90 Asp Ser 105 Leu Val Gly lie Gin Asp Ser Gly 170 Gly Lys 185 Glu Lys Gly Lys Lys Ile His Met 250 Thr Val 265 Ala Tyr Lys Asn PCT/GB01/02003 Met Arg Asn Gly Val Asp His Gin Val Gin Gly Ala Leu Asp 160 Glu Gly 175 Asp Gly Gly Lys Ser Ala Gly Asp 240 Arg Gly 255 Glu Arg Gin Ser Glu Lys WO 01/85772 Leu Asp Ala Asn PCT/GB01/02003 Ala Tyr Val Leu Glu Lys Glu Arg Tyr Set Ala Asp 305 310 315 Asp Gin Glu Lys Lys 385 Ala Tyr Leu Ser Glu 465 Arg Ile Phe Gln Ser Gly Thr Ile Thr Thr 340 Gln Thr Leu 355 Pro Gin Ala 370 Ala Thr Asp Lys Ala Tyr Ile Glu Ala 420 Arg Tyr Asp 435 Ser Asn Asn 450 His Trp Ser Leu Tyr Asp Ala Asp Gly 500 Asn Tyr Asn 515 Thr Ile Lys Gly 325 Arg Leu Phe Glu Arg 405 Ile Arg Leu Phe Ala 485 Thr Asp Asp Tyr Gly Lys Leu Glu 390 Leu His Phe Asn Ser 470 Leu Lys Gly Ala Asn Val .330 Phe Asn 345 Ile Asn Gin Phe Lys Asn Pro Thr 410 Asp Gly 425 Lys Thr Phe Gly His Asn His Gly 490 Arg Ala 505 Ala Ala Asn Pro Gly Asp Arg Ile 380 Glu Thr Thr Asp Ile 460 Ala Arg Asn Gly Asn 540 Pro Ser His 365 Glu Asn Asp Leu Gly 445 Trp Ser Gly Thr Ser 525 Arg Asn Arg 350 Gin Asp Glu Thr Thr 430 Lys Gin Arg lle Glu 510 Tyr His His 335 Leu Glu Lys Lys Gly 415 Gly Thr Pro Ser Ile 495 Ile Phe Asp 530 Val Ala 545 Val Arg Glu Ala Val Asn Ala Gly Tyr 550 555 383 Ile Lys Asn His WO 01/85772 Tyr Glu Leu Gly Val Ser Leu Leu Set 595 Thr Ala Ser 610 Trp Arg Gly 625 Gly Gln Lys Phe Gly Val Thr Leu Asn 675 Tyr Pro His 690 Asp Val Arg 705 PCT/GB1/02003 Gly Ala 565 His Ser 580 Ala Asn Leu Ala Arg Tyr Asp Arg 645 Asn Asp 660 Val Asn Ser Gin Leu Gly Ser Tyr Arg Thr Gly Gly Leu Thr Ala Lys Val 570 575 Pro Arg Phe 585 Glu Phe Gly 600 Arg Phe Gin 615 Gln Lys Ala Gly Lys Leu Phe Ala Asn 665 Ser Val Asn 680 Trp Thr Asn 695 Asn Tyr Lys Asp Lys Thr Trp Ile Gly Val Ala 640 Lys Gly 655 Lys Asp Phe Tyr Gly Arg <210> 197 <211> 2625 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(2625) <400> 197 atg aaa acc tcc gaa ctg cgc caa aaa ttc cta aaa ttt ttt gaa acc Met Lys Thr Ser Glu Leu Arg Gln Lys Phe Leu Lys Phe Phe Glu Thr 1 5 10 aaa ggc cac acc gtc gtc cgc tct tcc age etc gtg ccg cac gac gac 384 WO 01/85772 Lys Gly His cog aco ctg Pro Thr Leu tto tta ggt Phe Leu Gly aas tgo gta .Lys Cys Val tao ace 900 Tyr Thr Ala tto ggc gac Phe Gly Asp otg act too Leu Thr Sex 115 soc gtt tao Thr Val Tyr 130 ato ggt atg Ile Gly Met 145 gcg aan tao Ala Lys Tyr tgc ggc co Cys Gly Pro ggo ggo att Gly Gly Ile 195 Val ttt Phe gao Asp goa Ala cac His tte Phe gaa Giu gas Giu too Bar tcc Sex 165 toe Ser Gly Val aco Thr aaa Lys g Gly 70 050 His aas Lys tgg Trp, gac Asp gag Giu 150 gac Asp. ga 5Th agt Set Arg Bar sac gcg Asn Ala 40 cgc cog Arg Pro 55 ggc ass Gly Lys sace tto Thr Phe ogo gao Arg Asp etc aao Leu Asn 120 gao gaa Asp Giu 135 ego sto Arg Ile sac ttc Asn Phe att tto Ile Phe Cc gas Pro Giu 200 Ser Ser Leu Val Pro gge atg sac cag ttt Gly Met Asn Gin Phe tao ago ogo 900 aco Tyr Ser Arg Ala Thr 050 500 gao ttg ga His Asn Asp Leu Giu ttt gas atg atg ggc Phe Giu Met Met Gly gee ste oao tte get Ala Ile His Pta Ala 105 atecOct aa gac aa Ile Pro Lys Asp Lys 125 goc tao aao sto tgg Ala Tyr As Ile Trp 140 gto cgo ate ggc gao Val Arg Ile Sly Asp 155 tgg caas atg ggo gao Trp Gin Met Giy Asp 170 tao gao cac ggo ga Tyr Asp His Sly Giu 185 gas gao ggc gao ego Giu Asp Sly Asp Arg 205 PCT/GBO1/02003 Asp gta 144 Val aco gog oaa Ttr Ala Gin sac Asn aao Asn tgg Trp 110 otg Leu ttg Leu sac Asn aco Thr gaa Glu 190 tgg Trp stt tgg aso tgo gte ttt atg cag tto asc ogo gao gaa cas ggo sat WO 01/85772 Ile Trp Asn PCT/GBOl/02003 Cpa Val Plie Met Gin Phe Asn Arg Asp Glu Gin Gly Asn 210 215 220 atg Met 225 ogo Arg ttg Lou tto Phe too Ser ogo Arg 305 tao Tyr ttg Lou gc Mla aog Thr ggc Gip 385 ggt aac PAn ata Ile tto Phe aga Arg tgo Cys 290 ggc Gip aaa Lys gtc Val Oaa Gin ctg Lou 370 aaa Lys ttc cot tcc gto gat Pro 3cr Vai Asp oat His gc Ala ctg Lou 280 gao Asp att Ile cog Pro gc Ala aaa Lys 1 360 ttg I Lou I ato Ile gcc 386 ggt atg Gly mot aac tao PAn Tyr gaa aco Giu Thr goc gac Ala Asp 285 cct too Pro 3cr 300 gcc gtg Ala Val aaa cto Lys Lou ttg aaa Lou Lys ago ogt 3cr Arg 365 gog ctg Ala Lou 380 oto tao Lou Tyr cgo gaa 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 WO 01/85772 PCT/GB01/02003 Gly Phe Pro Tyr Asp Leu Thr Ala Asp Ile Cys Arg Glu Arg Asn Ile 405 410 415 gaa cog gac gaa gca ggc ttc gag egc gaa atg gaa gcc caa cgc gca 1296 Glu Pro Asp Glu Ala Gly Phe Glu Arg Glu Met Glu Ala Gln Arg Ala 420 425 430 cgc gca cgc gcc gcc ca ago ttc aaa gcc aac gee caa ctg cct tat 1344 Arg Ala Arg Ala Ala Gln Ser Phe Lys Ala Asn Ala Gin Leu Pro Tyr 435 440 445 gac ggt'caa gac ace gag ttt aaa ggt tat agc gaa cgc caa ace gaa 1392 Asp Gly Gln Asp Thr Glu Phe Lys Gly Tyr Ser Glu Arg Gln Thr Glu 450 455 460 tcc aaa gtc ctc gcc ctc tac aaa gac gge gag caa gtc aac gaa ttg 1440 Ser Lys Val Leu Ala Leu Tyr Lys Asp Gly Glu Gln Val Aan Glu Leu 465 470 475 480 aac gaa ggc gac agc ggc gca gtc gtc ate gac ttt acc ccg ttc tat 1488 Asn Glu Gly Asp Ser Gly Ala Val Val Ile Asp Phe Thr Pro Phe Tyr 485 490 495 gca gaa tcc ggc gge caa gtc ggc gat gtc gge tat atc ttc tca gge 1536 Ala Glu Ser Gly Gly Gln Val Gly Asp Val Gly Tyr Ile Phe Set Gly 500 505 510 gaa aac cgc ttt gaa gta cgc gat acc caa aaa atc aaa gcg gcc gta 1584 Glu Asn Arg Phe Glu Val Arg Asp Thr Gin Lys Ile Lys Ala Ala Val 515 520 525 ttc ggt caa ttc ggc gta caa act tca ggc cgt ctg aaa gtc ggc gac 1632 Phe Gly Gln Phe Gly Val Gln Thr Ser Gly Arg Leu Lys Val Gly Asp 530 535 540 agc gtt acc gee aaa gtg gac gac gaa atc cgc aat gcc aat atg cgc 1680 Ser Val Thr Ala Lys Val Asp Asp Glu Ile Arg Asn Ala Ann Met Arg 545 550 555 560 aac cac agc gca ace cac ttg atg cac aaa gec ctg cgc gat gta ttg 1728 Asn His Ser Ala Thr His Leu Met His Lys Ala Leu Arg Asp Val Leu 565 570 575 ggc aga cac gtc gaa caa aaa ggc tct ttg gtt acc gcc gaa teec acc 1776 Gly Arg His Val Glu Gln Lys Gly Ser Leu Val Thr Ala Glu Ser Thr 580 585 590 cgt ttc gac att tec cat ccc caa gcg gta act gcc gaa gaa att gcc 1824 WO 01/85772 Arg Phe Asp 595 PCT/GBOI/02003 Giu le-Mla Ile 5cr His Pro Gin Mla Val Thr Mla Giu 600 605 gaa cgo Glu A~rg goc att Ala Ile tto ggc Phe Gly ttc tct Phe 5cr 660 atc ggc Ile Gl? 675 cgc cgt Arg Arg gag caa Giu Gin aco gaa Thr Giu aaa gca Lys Mla 740 cac gca His Mla 755 aaa ctc Lys Leu gtc aac Vai Asn 615 ago atg 8cr Met 630 aaa tao Lys Tyr gaa ttg Glu Leu ttc aaa Phe Lys gaa gc Giu Ala 695 cgt ttg Arg Leu 710 gao gta Asqp Val gaa aaa GiU Lys gcc aaa Mla Lys gcc gcc Mla Ala 775 gtt gc Val Ala ggc gcg Gly Ala ctg caa Leu Gin 655 tca ogc 5cr Arg 670 att g00 Ile Ala ctc aaa Leu Lys gaa acc Giu Thr ggc gog Gly Mla 735 goc ga& Ala Glu 750 gac ttg Asp Leu goc gc Ala Ala 1872 1920 1968 2016 2064 2112 2160 2208 2256 2304 2352 2400 ttg gda cgc gc Leu Ala Arg Ala 745 ttg gac gat goa Lou Asp Asp Mla atc gaa gcc gao gca Ile Glu Mla Asp Ala 780 cgc gaa etc gtt acc gat tta acc ggt aaa too gao aao goc gtg att WO 01/85772 Arg Glu Ile 785 ett tta gcg Leu Leu Ala tcc aaa ccg ttg Set Lys Pro Leu 820 gca gcc gaa caa Ala Ala Glu Gin 835 caa gcc ggc ggec Gln Ala Gly Gly 850 gtg aaa gac tgg Val Lys Asp Trp 865 <210> 198 <211> 874 <212> PRT <213> Neisseria <400> 198 Met Lys Thr Ser 1 Lys Gly His Thr Pro Thr Leu Leu Phe Leu Gly Phe Lys Cys Val Arg Tyr Thr Ala Arg Ser Asp Asn 795 tcc ctg tge Ser Leu Cys ggc gat ctg Gly Asp Leu ggc aga cca Gly Arg Pro 845 ccc gcc gtg Pro Ala Val 860 tga 875 PCT/GB01/02003 Ala Val Ile 800 gcc ggc gta 2448 Ala Gly Val 815 gtt aaa ttt 2496 Val Lys Phe 830 gat ttg gcg 2544 Asp Leu Ala ttg gat agc 2592 Leu Asp Ser 2625 meningitidi Glu Leu Arg i 5 Val Val Arg Phe Thr Asn Asp Lys Arg 1 55 Ala Gly Gly 70 His His Thr Lys Phe Ser Set 25 Gly Met Tyr Ser His Asn Phe Glu Leu Lys Leu Val Asn Gln Arg Ala Asp Leu Met Met Phe Phe Pro His Phe Lys Thr Thr GlU Asn Gly Asn WO 01/85772 Phe Gly Asp Leu Thr Ser 115 Thr Val Tyr 130 Ile Gly Met 145 Ala Lys Tyr Cys Gly Pro Gly Gly Ile 195 Ile Trp Asn 210 Met Asn Pro 225 Arg Ile Ala Leu Phe Gin Phe Arg Met 275 Ser Cys Ser 290 Arg Gly Tyr 305 Tyr Lys Leu Leu Val Lys Ala Ile 105 Ile Pro Ala Tyr Val Arg Trp Gin 170 Tyr Asp 185 Glu Asp Phe Asn Val Asp Val His 250 Val Ala 265 Lys Val Gly Val Ile Arg Phe Phe 330 Tyr Pro 345 PCT/GB01/02003 Glu Phe Leu Ala Asn Glu Lys Gly 160 Gly Pro 175 Ile Trp Ile Glu Gly Asn Leu Glu 240 Ile Asp 255 Ala Pro Ile Arg Glu Gly His Gly 320 Ala Asp 335 Lys Gin WO 01/85772 Ala Gln ile PCT/GB01/02003 Glu Glu Ala Leu Lys Asn Glu Glu Ser Arg Phe Ala Gin 355 360 365 Thr Gly 385 Gly Glu Arg Asp Ser 465 Asn Ala Glu Phe Ser 545 Asn Gly Gly Met Ala 375 Gly Gly Glu 390 Asp Leu Thr 405 Ala Gly Phe Ala Gln Ser Thr Glu Phe 455 Ala Leu Tyr 470 Ser Gly Ala 485 Gly Gin Val Glu Val Arg Gly Val Gin 535 Lys Val Asp 550 Thr His Leu 565 Glu Gln Lys Leu Val Tyr Asp Glu Arg Ala Gin 430 Gin Leu 445 Arg Gin Val Asn Thr Pro Ile Phe 510 Lys Ala 525 Lys Val Ala Asn Arg Asp Ala Glu 590 Arg Phe Asp 595 Ile Ser His Pro Gin Ala Val Thr Ala Glu 600 605 391 Glu Ile Ala WO 01/85772 Glu Val Glu 610 Asn Ala Ala 625 Met Leu Phe Gly Gly Phe Gly Asp Ile 675 Gly Val Arg 690 Ala Gin Glu 705 Ala Gin Thr His Ala Lys Ala Val His 755 Ala Ala Lys 770 Arg Glu lie 785 Leu Leu Ala Ser Lys Pro Ala Ala Glu 835 Gin Ala Gly 850 Val Asn 615 Ser Met 630 Lys Tyr Glu Leu Phe Lys Glu Ala 695 Arg Leu 710 Asp Val Glu Lys Ala Lys Ala Ala 775 Asp Leu 790 Asn Asp Gly Lys Gly Gly Asp Ala 855 Leu Ala 620 Gin Lys 635 Val Arg Thr His Glu Gly Leu Asn 700 Ile Ile 715 Ile Gin Arg Ala Asp Ala Ala Asp 780 Ser Asp 795 Ser Leu Gly Asp Gly Arg PCT/GB01/02003 Asn Val Ala Val Thr Gly Ala Met 640 Val Leu Gln Met 655 Val Ser Arg Thr 670 Gly Ile Ala Ala 685 Ala Leu Lys Trp Ala Glu Thr Lys 720 Ala Gly Ala Ala 735 Lys Ala Glu Leu 750 Lys Asp Leu Gly 765 Ala Ala Ala Leu Asn Ala Val Ile 800 Cys Ala Gly Val 815 Leu Val Lys Phe 830 Pro Asp Leu Ala 845 Lys Leu Pro Ala Val Leu Asp Ser 860 WO 01/85772 WO 0185772PCT/GBO1/02003 Val Lys Asp Tly Val Gly Ala Lys Leu Val 865 870 <210> 199 <211> 207 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (207) <400> 199 gtg caa aga ggo ogt ctg aaa ggt ctc gtt tgo cgt egg ttg ggt cgc Val Gin Arg Gly Arg Leu Lys Gly Leu Val. Cys Arg Arg Leu Gly Arg gac con eon Asp Pro Thr ttt gtg aag tat aaa aa 't gtt ggt cat gao oca ecc Phe Val. Lys Tyr Lys Asn Vel Gly His Asp Pro Thr tao otg oct ttt tgt ace eag agg cta tct gaa agg cot tgt ttg cog Tyr Leu Pro Phe Cys Thr Lys Arg Leu 9cr Glu Arg Pro Cys Leu Pro tat ggt Tyr Gly s0 ggg tog a cOO Gly 9cr Arg Pro ega ttt tta tta Arg Phe Leu Leu ggg tat Gly Tyr gao con agc Asp Pro 9cr tac tig ote oga taa Tyr Leu Leu Arg <210> 200 <211> 68 <212> PRT <213> Neisseria meniLngitidis <400> 200 Val. Gln Arg Gly Arg Leu Lys Gly 1 5 Asp Pro Thr Asn Phe Val. Lys Tyr Len Val Cys Arg Arg Leu Gly Arg 10 Lys Asn Val. Gly His Asp Pro Thr WO 01/85772 PCT/GB01/02003 Tyr Leu Pro Phe Cys Thr Lys Arg Leu Ser Glu Arg Pro Cys Leu Pro 40 Tyr Gly Gly Ser Arg Pro Ser Arg Phe Leu Leu Gly Tyr Asp Pro Ser 55 Tyr Leu Leu Arg <210> 201 <211> 2277 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(2277) <400> 201 atg gca caa act aca etc aaa ccc att gtt tta tea att ctt tta atc 48 Met Ala Gin Thr Thr Leu Lys Pro Ile Val Leu Ser Ile Leu Leu Ile 1 5 10 aac aca ccc etc etc gcc caa gcg cat gaa act gag caa tcg gtg gat 96 Asn Thr Pro Leu Leu Ala Gin Ala His Glu Thr Glu Gin Ser Val Asp 25 ttg gas acg gte ago gtc gtc ggc aaa age cgt ccg cgc gcc acg tog 144 Leu Glu Thr Val Ser Val Val Gly Lys Ser Arg Pro Arg Ala Thr Ser 40 ggg ctg ttg cac act tcg ace gcc tec gac aaa ate ate tcc ggc gat 192 Gly Leu Leu His Thr Set Thr Ala Ser Asp Lys Ile Ile Ser Gly Asp 55 ace ttg cgc caa aaa gcc gtc aac ttg gge gac get tta gac ggc gta 240 Thr Leu Arg Gin Lys Ala Val Asn Leu Gly Asp Ala Leu Asp Gly Val 70 75 ccg ggc ate cac get tcg caa tac ggc ggc ggc gcg tct get ccc gtc 288 Pro Gly Ile His Ala Ser Gin Tyr Gly Gly Gly Ala Ser Ala Pro Val 90 att cge ggt caa aca ggc agg egg att aaa gtg ttg aac cat cac gge 336 TIe Arg Gly Gin Thr Gly Arg Arg Ile Lys Val Leu Asn His His Gly 100 105 110 WO 01/85772 gaa sos ggc Glu Thr Gly 115 PCT/GB01/02003 gat atg gcg gat Asp Met Ala Asp tcg ccc gat cac Ser Pro Asp His att atg gta Ile Met Val gat ace Asp Thr 130 gc ttg tog caa tag Ala Leu 3cr Gin Gin gtc gaa ato ctg Val Glu le Leu cgo ggg cog gtt acg Arg Sly Pro Val Thr 140 gtc gat gtt goc gat Val Asp Val Ala Asp otc ttg tsc agc tcg Len Len Tyr Ser Ser 145 ggo aaa ato ccc gaa Gly Lys Ile Pro Glu 165 ggo aat gtg gcg ggg Sly Asn Val Ala Sly 150 ass atg cct gaa aac Lys Met Pro Glu Asn ggo gta tag ggo Gly Val Ser Gly gaa cto Gin Len 175 gga ttg cgt Gly Len Arg ago ago ggc sat ctg gas aaa ctc acg Ser Ser Gly Amn Len Gin Lys Lcu Tbr 185 ate sat atc ggt ttg ggc Ile Asn Ile Sly Len Gly 195 tat cgo asa tog ggg gat Tyr Arg Lys Ser Sly Asp 210 aaa aac Lys Asn 200 ttt gta ttg cac Phe Val Len His te ggo ggo Ser Gly Gly 190 gaa ggg ctg Glu Gly Leu sat ctg aaa Asn Len Lys gcc gta ccg cgt Ala Val Pro Arg tao ego Tyr Arg 220 ctg coo gao ago Leu Pro Asp Ser gcc gat tog can Ala Asp Ser Gln ggc ago ato ggg Sly ser lie Gly tot tgg gtt ggc Ser Trp Val Gly aaa ggt ttt atec ggc gts gog tac ago Lys Gly Phe Ile Gly Val Ala Tyr Set 250 gac cgt Asp Arg 255 ego gac caa Arg Asp Gin cac goc gao His Ala Asp 275 ggt otg cot gco Gly Lou Pro Ala ago cac gaa tao Ser His Glu Tyr gat gat tgo Asp Asp Cys 270 ogo tat tta Arg Tyr Len ato ato tgg can Ile Ile Trp Gin ago ttg att sac Se Leu Ile Asn cag ott tat cog cac ctg Gin Leu Tyr Pro His Leu 290 ace gsa gas ga atec gat tao gao sat Thr Glu Giu Asp Ile Asp Tyr.Asp Asn 300 WO 01/85772 PCT/GB01/02003 ggc ttg ago tgc Gly Leu Ser Cys ggc ttc cac gac Gly Phe His Asp gac gat Asp Asp 315 aat gea cac Ann Ala His gca cac Ala His 320 ace cac agc ggc Thr His Ser Gly cog tgg ata gac ctg cgc aac aaa cgc Pro Trp Ile Asp Leu Arg Asn Lys Arg 330 tac gaa 1008 Tyr Glu 335 ctc cgt gec Leu Arg Ala gta cac ctg Val His Leu 355 tgg aag caa cog Trp Lys Gn Pro ttc ccc ggt ttt Phe Pro Gly Phe 345 cgc cac gac gaa Arg His Asp Glu gaa gcc ctg egc Glu Ala Leu Arg 350 1056 1104 aac cgc aac gac Asn Arg Asn Asp gca ggc gat Ala Gly Asp gca gtc Ala Val 370 gaa aac ttt ttt Glu Asn Phe Phe cac caa ccc ata His Gin Pro Ile 390 aac caa acg caa Asn Gln Thr Gin aac gcc cgc ate gag Asn Ala Arg Ile Glu 380 age tgg ggc gtg caa Set Trp Gly Val Gin 400 1152 1200 ego Arg ggt cgt ctg aaa Gly Arg Leu Lys tat tta caa caa Tyr Leu Gin Gln teoo agt gct tta tet gcc ata tcc gaa Ser Ser Ala Leu Set Ala Ile Ser Glu 410 gcg gtt Ala Val 415 1248 aaa caa ccg Lys Gin Pro ggt gta gaa Gly Val Glu 435 ctg ctt gac aac Leu Leu Asp Asn aaa gtg Lys Val 425 caa cat tac Gln His Tyr age ttt ttc Ser Phe Phe 430 gga ggc gta Gly Gly Val 1296 cag gca aac tgg gac aac ttc acg ctt Gin Ala Asn Trp Asp Asnn Phe Thr Leu 440 1344 cgo gtg Axg Val 450 gat cgg Asp Arg 465 gaa aaa caa aaa Glu Lys Gin Lys toc att cag Set Ile Gin tac gac Tyr Asp 460 ccc gac Pro Asp 475 aaa gca ttg att Lys Ala Leu Ile 1392 1440 gaa aac tac Glu Asn Tyr aac cac cccctg Asn His Pro Leu ctc ggc gcg Leu Gly Ala cgc caa acc gcc Arg Gin Thr Ala cgc-tca ttc gca ctt tcg ggc aac tgg tat Arg Ser Phe Ala Leu Set Gly Asn Trp Tyr ttc acg Phe Thr 495 1488 WO 01/85772 oca cas cao Pro Gin His PCT/GBO1/02003 sa otc ago ctg aco goc too cat cag gaa cge etg cog 1536 Lys Lou 3cr Lou Thr Ala 5cr His Gin Glu Arg Lou Pro toa ttt Phe gas Giu 545 etc Leu gac Asp gtg Val atc Ile tat Tyr 625 gat Asp coo Pro ace Thr sog Tb: gaa Glu 530 etc Leu tac Tyr .gga Gly ogo Arg tac Tyr 610 gts Val gc Ala ogt Arg gao Asp cas. Gin 515 gtc Val gag Ala Arg ogo Arg tac Tyr 595 ttc Pho egs Arg tao Tyr gtt Val Cgt Arg 675 S00 gag Giu gge Gly otg Lou sac Asn ggc Giy 580 sac Asn sa Lys ggo Gly ggc Gly cog Pro 660 eta Ile otg tao goa Lou Tyr Ala aac aaa cao Asn Lys His 535 ggo tao ga Gly Tyr Glu 550 ogo ttc ggt Arg Pho Gly 565 ccc saa too Pro Lys 3cr eaa too ggc Gin 8cr Gly cog aca cog Pro Thr Pro 615 cgt otg aaa Arg Lou Lys 630 aso ogt cot Asn Arg Pro 645 got geg ego Ala Ala Arg gat goc aat Asp Ala Asn 505 cac ggc His Gly 520 ote sac Lou Asn ggo gao Gly Asp aso tao Asn Tyr atc gas Ile Giu 585 gco gao Ala Asp 600 ogo: tao Arg Tyr sac otg Asn Lou tta stc Pho Ile otc ggo Lou Gly 665 ttg gao Lou Asp 680 397 gte gc Vai Ala 525 cgt tee Arg 3cr 540 caa tao Gin Tyr gee cas Ala Gin ago gas 5cr Giu ggc gag Gly Ala 605 ggo gtt Giy Val 620 eta ccc Lou Pro gae gao Asp Asp otg as Leu Ly7s ago gtg Arg Val 685 sac cc Asn Tb: sat ate Asn Ile otg gos Lou Ala 560 tta sac Lou Asn 575 sag ate Lys Lou ggo gas G2Ly Giu ggo gao .Gly Asp aga gas Arg Giu 640 aat gc Asn Ala 655 tog otg 5cr Lou goc cas Ala Gin 1584 1632 1680 1728 1776 1824 1872 1920 1968 2016 2064 WO 01/85772 &ac Asn aac Asn 705 tgg Trp age Gly cgc tac gac aeg cgc acg Arg Tyr Giu flit Arg Tin 695 aac tac cgc cgc aat acg Asn Tyr Arg Arg Asn Thr 710 gcc gao aae ctg etc aae Ala Asp Asn Lou Leu Asn 725 730 tct gat acg cog caa atg 8cr Asp Thr Pro Gin Met 745 aag ttt taa Lys Phe ccc gga Pro Gly 700 cgc tat Arg Tyr 715 caa tec Gin Ser ggc cgc Gly Arg PCT/GBOI/02003 I etc 2112 Leu aat 2160 >Asn 720 ;cac 2208 tHis ggc 2256 Gly 2277 <210> 202 <211> 758 <212> PRT <213> Neisseria <400> 202 Met Ala Gin Thr 1 Asn Thr Pro Leu Leu Giu Thr Val Gly Leu Leu His flit Leu Arg Gin Pro Gly Ile His Ile Arg Gly Gin 100 meningitidis Thr Leu Lys Pro 5 Leu Ala Gin Ala 5er Val Val Gly Thr Ser Thr Ala Lys Ala Val Asn 70 Ala Ser Gin Tyr Thr Gly Arg Arg 398 Leu Ile Val Asp Tin Ser Gly A-sp Gly Val Pro Val His Gly WO 01/85772 Glu Thr Gly Asp Met 115 Asp Thr Ala Leu Ser 130 Leu Leu Tyr Ser Ser 145 Gly Lys Ile Pro Glu 165 Gly Leu Arg Leu Ser 180 Ile Asn Ile Gly Leu 195 Tyr Arg Lys Ser Gly 210 Arg Leu Pro Asp Ser 225 Ser Trp Val Gly Glu 245 Arg Asp Gin Tyr Gly 260 His Ala Asp lie Ile 275 Gin Leu Tyr Pro His 290 Pro Gly Leu Ser Cys 305 Thr His Ser Gly Arg 325 Leu Arg Ala Glu Trp I 340 Val His Leu Asn Arg I 355 Ala Gin Gly 150 Lys Ser Gly Asp His 230 Lys Leu Trp Leu Gly 310 Pro Lys Asn I Asp Phe Ser 120 SGin Val Glu 135 Asn Val Ala Met Pro Glu Gly Asn Leu 185 Lys ASn Phe 200 Tyr Ala Val 215 Ala Asp Set Gly Phe Ile Pro Ala His 265 Gln Lys Ser S280 Leu Thr Glu 295 Phe His Asp Trp Ile Asp Gin Pro Phe I 345 Asp Tyr Arg I 360 Pro Ile Gly Asn 170 Glu Val Pro Gin Gly 250 Set Leu Glu Asp Leu 330 Pro Asp Leu Leu 155 Gly Lys Leu Arg Thr 235 Val His Ile Asp Asp 315 Arg J Gly His Arg 140 Val Val Leu His Tyr 220 Gly Ala Glu Asn Ile 300 Asn Asn Phe SAla 125 Gly Asp Ser Thr Thr 205 Arg Ser Tyr Tyr Lys 285 Asp Ala Lys Glu Ile Pro Val Gly Ser 190 Glu Asn Ile Ser Asp 270 Arg Tyr His Arg Ala PCT/GB01/02003 Met Val Val Thr SAla Asp 160 SGlu Leu 175 Gly Gly Gly Leu Leu Lys Gly Leu 240 Asp Arg 255 Asp Cys Tyr Leu Asp Asn Ala His 320 Tyr Glu 335 Leu Arg 350 is Asp Glu Lys Ala Gly Asp 365 WO 01/85772 Ala Val Glu Ann 370 Leu Arg His Gin 385 Tyr Leu Gln Gln Lys Gln Pro Met 420 Gly Val Glu Gin 435 Arg Val Glu Lys 450 Asp Arg Glu Asn 465 Arg Gin Thr Ala Pro Gln His Lys 500 Ser Thr Gin Glu 515 Phe Glu Val Gly 530 Glu Leu Ala Leu 545 Leu Tyr Arg Asn Asp Gly Arg Gly 580 Val Arg Tyr Asn 595 Ile Tyr Phe Lys Phe Asn Asn 375 Ile Gly Arg 390 Ser Ser Ala Leu Asp Asn Asn Trp Asp 440 Lys Ala Ser 455 Tyr Asn His 470 Ser Phe Ala Ser Leu Thr Tyr Ala His 520 Lys His Leu 535 Tyr Glu Gly 550 Phe Gly Asn Lys Ser Ile Ser Gly Ala 600 Thr Pro Arg 615 Ala Arg Ile Trp Gly Val Ser Glu Ala 415 Tyr Ser Phe 430 Glu Gly Gly 445 Lys Ala Leu Leu Gly Ala Trp Tyr Phe 495 Glu Arg Leu 510 Ala Thr Asn 525 Ser Asn Asn Tyr Asn Leu Gin Thr Leu 575 Glu Met Lys 590 Ala Glu Gly 605 Val Ser Gly PCT/GB01/02003 Glu Gin 400 Val Phe Val Ile His 480 Thr Pro Thr Ile Ala 560 Asn Leu Glu Asp WO 01/85772 Tyr Val Arg Gly 625 Asp Ala Tyr Gly Pro Arg Val Pro 660 Thr Asp Arg Ile 675 Asn Lys Leu Ala 690 Ann Leu Gly Ala 705 Trp Tyr Val Lys Ser Ser Phe Leu 740 Gly Val Asn Val 755 Amn Leu Pro Phe Ile Ala 650 Leu Gly Phe 665 Leu Asp Tyr 680 Thr Arg Thr Arg Ann Thr Leu Leu Asn 730 Pro Gln Met 745 Set Leu Pro 635 Gln Asp Asp His Leu Lys Tyr Arg Val 685 Pro Gly His 700 Arg Tyr Gly 715 Gln Ser Val Gly Arg Ser Gly Arg Gln Asn 655 Ala Ser 670 Phe Ala His Met Glu Trp Tyr Ala 735 Phe Thr 750 PCT/GB01/02003 Glu 640 Ala Leu Gln Leu Asn 720 His Gly <210> 203 <211> 225 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 203 atg agg ctg gca acc aag gat ttg gcg gaa gcc att agg aaa gga cag Met Arg Leu Ala Thr Lys Asp Leu Ala Glu Ala Ile Arg Lys Gly Gin 1 5 10 gtt cgc aaa tca agc ttt aac aca gaa caa tta agg gca att gaa aaa Val Arg Lys Ser Ser Phe Asn Thr Glu Gln Leu Arg Ala Ile Glu Lys 25 WO 01/85772 PCT/GB01/02003 gga gaa tct aaa ata ccg gat tac act tgg cat cat cat caa gat aca 144 Gly Glu Ser Lys Ile Pro Asp Tyr Thr Trp His His His Gin Asp Thr 40 gga agg atg caa ttg att cgt gaa ggc ttg cat cat gat ace ggc cat 192 Gly Arg Met Gin Leu Ile Arg Glu Gly Leu His His Asp Thr Gly His 55 att ggt tgg gaa gca atg aac aaa gga agg taa 225 Ile Gly Trp Glu Ala Met Asn Lys Gly Arg 70 <210> 204 <211> 74 <212> PRT <213> Neisseria meningitidis <400> 204 Met Arg Leu Ala Thr Lys Asp Leu Ala Glu Ala Ile Arg Lys Gly Gin 1 5 10 Val Arg Lys Ser Ser Phe Asn Thr Glu Gin Leu Arg Ala Ile Glu Lys 25 Gly Glu Ser Lys Ile Pro Asp Tyr Thr Trp His His His Gin Asp Thr 40 Gly Arg Met Gin Leu Ile Arg Glu Gly Leu His His Asp Thr Gly His 55 Ile Gly Trp Glu Ala Met Asn Lys Gly Arg <210> 205 <211> 1176 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> (1)..(1176) <400> 205 atg gca aaa atg atg aaa tgg gcg get gtt gcg gcg gtc gcg gcg gca 48 Met Ala Lys Met Met Lys Trp Ala Ala Val Ala Ala Val Ala Ala Ala 402 WO 01/85772 1 gog gtt tgg Ala Val Trp tat att acg Tyr Ile Tb: gca aca ggg Ala Thr Gly gon tog ggg Ala 8cr Gly aaa aag ggc Lys Lye Gly aog oto aat Thr Leu Asn gtg tog gon VTai Ser Ala 115 cag gog gog Gin Ala Ala 130 ago goa cag Ser Ala Gin 145 aag got cta Lye Ala Leu gaa ttg ggc Giu Leu Gly gog att oto coo gag cog cag Pro Giu Pro Gin ato ago cgg acg Ile 5cr Arg Thr gta tcg gto ggo Val Set Val Gly aaa oto ggg can Lye Leu Gly Gin 75 tog aoo tog oag Set Tb: 5cr Gin acg tat cag gog Tb: Tyr Gin Ala 110 gag aag aaa tat Glu Lys Lys Tyr 125 got aaa gaa gat Ala Lys Giu Asp 140 goc aat gtt gcc Ala Asn Val Ala 155 ato aut aoo goo Ile Asn Tb: Ala atg gao ggo aog Met Asp Giy Thr 190 aac gog gog cag PCT/GBOI/02003 got got 96 Ala Ala gtt tot 144 Val Set gog ong 192 Ala Gin ong gtt 240 Gin Val s0 aco aat 288 Th: Asn aag otg 336 Lye Leu aag ogt 384 Lys Arg ttg gaa 432 Leu Giu gag otg 480 Giu Leu 160 gag tog 528 Glu Ser 175 gtg gtg 576 Val Val tot aog 624 Ala Ile Leu Val. Giu (flu (fly Gin Tb: Val. Asn Ala Ala Gin 5cr Tb: WO 01/85772 WO 0185772PCT/GBO1/02003 cog aeg Pro Thr 210 att gto aa ttg gag eat ctg gat atg atg ttg aaa aaa atg Ile Val Gin Leu Ala Asn Leu Asp Met Met Lieu Ann Ly's Met att gao gag ggc gat att acc aag gtg aag geg Ile Ala Glu Guy Asp Ile Tin Ly's Val Ly's Mla 230 235 ggg cag gat Guy Gin Asp tog ttt ag att ttg tce gaa cog gat acg cog att aag geg Sar Phe Thr lie Leu Ser Giu Pro Asp Thr Pro Ile Lys Mla aag oto LY'S Leu 255 gac ago gto Asp 3cr Val age agt aog Ser Bar Thr 275 coo ggg ctg ac Pro Guy Lou Thr atg tcg tcg gga Met 3cr 3cr Guy ggc tac sac Guy Tyr An 270 gat aog got too Asp Thr Ala Bar gag gtc tao tat tat gao cgt tog Mla Val Tyr Tyr Tyr Ala Arg'3cr 285 ttt gtg ccg aat cog gao ggc aaa dc gao aag Phe Val Pro Ann Pro Asp Gli' Ly's Lou Mla Thr 290 295 aat ag gtt gaa ate gao ggt gtg aaa eat gtg Asn Thr Val Gi Ile Asp Cii' Vai Ly's Ann Val 305 310 315 ggg atg aog acg cag Gli' Met Thr Thr Gin 300 otg att att cog tog Lou Ile Ile Pro 3cr atg aco gtg Lou Thr Val gos gae ggo Ala ASP Guy agt atg sat 3cr Met An 355 sea sat Ly's An 325 aag gog Ly's Mla 340 ago ggc gga agg Arg Gli' Gli Arg ttt gtg ago gtg Phe Val Arg Val ttg ggt Leu Gli' 335 1008 gag gaa ago Ala Gin Arg eta agg ace ggt Ile Arg Thr Cii' etg age gao Met Arg Asp 350 gao as gtg Asp Lye Val 1056 1104 &co gas gta sa Thr Giu Val Ly's ggg ttg sea gag Cii' Lou Ly's Gin gto ate Val Ile 370 too gaa eta ac gao gao gag cag cag 5cr Gi Ile Thr Ala Mla Gin Gin Gin 3175 ago gga gas ago 5cr Cii' Giu Arg 1152 gee eta ggo gga oog Ala Leu Guy Cli' Pro cog ago oga Pro Arg Arg 1176 WO 01/85772 385 <210> 206 <211> 392 <212> PRT <213> Neisseria PCT/GB01/02003 390 meningitidis <400> 206 Met 1 Ala Tyr Ala Ala Lys Thr Val Gin Set 145 Lys Glu Ala Lys Met Met 5 Trp Gly Gly Thr Glu Thr Gly Glu Ile Gly Gin Ile Gly Asp Leu Asn Thr Glu 100 Ala Gin Ile 115 Ala Leu Trp Gin Asp Ala Leu lie Arg 165 Gly Tyr Thr 180 Leu Val Glu 195 Ala Tyr Arg 40 Ser Leu Glu Lys Gly 120 Asp Ala Lys Thr Gin 200 Ala Val Ala Glu Pro Gin Ser Arg Thr Ser Val Gly Leu Gly Gin Thr Ser Gin Tyr Gin Ala 110 Lys Lys Tyr 125 Lys Glu Asp 140 Asn Val Ala Asn Thr Ala Asp Gly Thr 190 Ala Ala Gin 205 Ala Ala Ala Ala Val Set Ala Gln Gin Val Thr Asn Lys Leu Lys Arg Leu Glu Glu Leu 160 Glu Ser 175 Val Val Ser Thr WO 01/85772 PCT/GB01/02003 Pro Thr Ile Val Gin Leu Ala Asn Leu Asp Met Met Leu Asn Lys Met 210 215 220 Gin Ile Ala Glu Gly Asp Ile Thr Lys Val Lys Ala Gly Gln Asp Ile 225 230 235 240 Ser Phe Thr Ile Leu Ser Glu Pro Asp Thr Pro Ile Lys Ala Lys Leu 245 250 255 Asp Ser Val Asp Pro Gly Leu Thr Thr Met Ser Ser Gly Gly Tyr Asn 260 265 270 Ser Ser Thr Asp Thr Ala Ser Asn Ala Val Tyr Tyr Tyr Ala Arg Ser 275 280 285 Phe Val Pro Asn Pro Asp Gly Lys Leu Ala Thr Gly Met Thr Thr Gin 290 295 300 Asn Thr Val Glu Ile Asp Gly Val Lys Asn Val Leu Ile Ile Pro Ser 305 310 315 320 Leu Thr Val Lys Asn Arg Gly Gly Arg Ala Phe Val Arg Val Leu Gly 325 330 335 Ala Asp Gly Lys Ala Ala Glu Arg Glu Ile Arg Thr Gly Met Arg Asp 340 345 350 Ser Met Asn Thr Glu Val Lys Ser Gly Leu Lys Glu Gly Asp Lys Val 355 360 365 Val Ile Ser Glu Ile Thr Ala Ala Glu Gin Gln Glu Sar Gly Glu Arg 370 375 380 Ala Leu Gly Gly Pro Pro Arg Arg 385 390 <210> 207 <211> 342 <212> DNA <213> Neisseria meningitidis <220> <221> CDS <222> <400> 207 WO 01/85772 ttg ttt gta tgc Leu Phe Val Cys 1 atc egg ttt tgc Ile Arg Phe Cys tcg gat gtg gtt Sex Asp Val Val can tat att ttt Gin Tyr Ile Phe cag gta agg gag Gin Val Arg Glu acg gaa tat cgg Thr Glu Tyr Arg sag gca ant tcg Lys Ala Asn Ser 100 gtc tgt Val Cys <210> 208 <211> 114 <212> PRT <213> Neisserin <400> 208 Leu Phe Val Cys 1 Ile Arg Phe Cys Sex Asp Vai Val Gin Tyr Ile Phe PCT/GBO1/02003 ttt gas aaa tgt ttg ttt ccg gat ttt gee att ccc 48 Phe Giu Lys Cys Leu Pie Pro Asp Phe Ala Ile Pro 5 10 tta gcg egg act Leu Ala Arg Thr meningitidis Phe Glu Lys Cys Leu Phe Pro Asp Phe Ala Ile Pro 5 10 Ala Val Arg Cys Val Len Ala Arg Thr Cys Ser Lys 25 Pro Val Phe Gly Ala Leu Ile Arg Asp Ala Asp Phe 40 Len Sex Tyr Asa Asn Glu Gly Len Met Ser Val Gly 407 WO 01/8 Gin Val Thr Glu Lys Ala Val Cys 5772 PCT/GB01/02003 55 Arg Glu Ile Phe Glu Arg Phe Gly Lys Tyr Asn Leu Val Gin 70 75 Tyr Arg Arg Phe Lys Ala Asp Lys Thr Glu Asn Arg Asn His 90 Asn Ser lie Phe Glu Tyr Leu His Glu Thr Phe Ala Lys lie 100 105 110 <210> 209 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence:Primer Sequence <400> 209 ttggttaatt ggttgtaaca ctgg <210> 210 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence:Primer Sequence <400> 210 attctcatgt ttgacagcg <210> 211 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence:Primer Sequence 408 WO 01/85772 <400> 211 atcctacaac ctcaagct <210> 212 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence:Primer Sequence <400> 212 atcccattct aaccaagc <210> 213 <211> 19 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence:Primer Sequence <400> 213 aagagattac gcgcagacc <210> 214 <211> 18 <212> DNA <213> Artificial Sequence <220> <223> Description of Artificial Sequence:Primer Sequence <400> 214 aatacgcaac cgcctctc PCT/GB01/02003 18 18 19 18