AU2002222193A1 - Methods and kits for detecting protein kinases - Google Patents

Methods and kits for detecting protein kinases

Info

Publication number
AU2002222193A1
AU2002222193A1 AU2002222193A AU2219302A AU2002222193A1 AU 2002222193 A1 AU2002222193 A1 AU 2002222193A1 AU 2002222193 A AU2002222193 A AU 2002222193A AU 2219302 A AU2219302 A AU 2219302A AU 2002222193 A1 AU2002222193 A1 AU 2002222193A1
Authority
AU
Australia
Prior art keywords
atp
tested
kits
methods
protein kinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002222193A
Inventor
Sharon Patricia Mary Crouch
Kevin John Slater
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cambrex Bio Science Nottingham Ltd
Original Assignee
Lumitech UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=9905234&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2002222193(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lumitech UK Ltd filed Critical Lumitech UK Ltd
Publication of AU2002222193A1 publication Critical patent/AU2002222193A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A method for measuring protein kinase activity comprising: (a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested; (b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a); (c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and (d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested.
AU2002222193A 2000-12-15 2001-12-13 Methods and kits for detecting protein kinases Abandoned AU2002222193A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0030727.2A GB0030727D0 (en) 2000-12-15 2000-12-15 Methods and kits for detecting kinase activity
GB0030727.2 2000-12-15
PCT/GB2001/005506 WO2002048390A2 (en) 2000-12-15 2001-12-13 Methods and kits for detecting protein kinases

Publications (1)

Publication Number Publication Date
AU2002222193A1 true AU2002222193A1 (en) 2002-06-24

Family

ID=9905234

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002222193A Abandoned AU2002222193A1 (en) 2000-12-15 2001-12-13 Methods and kits for detecting protein kinases

Country Status (12)

Country Link
US (3) US6599711B2 (en)
EP (1) EP1341928B1 (en)
JP (1) JP3854927B2 (en)
AT (1) ATE310101T1 (en)
AU (1) AU2002222193A1 (en)
CA (1) CA2431274C (en)
CH (1) CH694096A5 (en)
DE (2) DE60115090T2 (en)
DK (1) DK1341928T3 (en)
ES (1) ES2256165T3 (en)
GB (2) GB0030727D0 (en)
WO (1) WO2002048390A2 (en)

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US7355010B2 (en) * 2003-01-30 2008-04-08 Bellbrook Labs, Llc Assay method for group transfer reactions
US7332278B2 (en) * 2003-01-30 2008-02-19 Bellbrook Labs, Llc Assay methods for group transfer reactions
US8088897B2 (en) * 2003-01-30 2012-01-03 BellBrook Labs, Inc. Assay method for group transfer reactions
US7378505B2 (en) * 2003-01-30 2008-05-27 Bellbrook Labs Llc Assay method for group transfer reactions
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
CA2445420A1 (en) * 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US20050137147A1 (en) * 2003-12-22 2005-06-23 Alcon, Inc. Agents for treatment of diabetic retinopathy and drusen formation in macular degeneration
TW200526224A (en) * 2003-12-22 2005-08-16 Alcon Inc Short form c-Maf transcription factor antagonists for treatment of glaucoma
DK1696958T3 (en) * 2003-12-22 2007-07-09 Alcon Inc Agents for the treatment of glaucomatous retinopathy and optic neuropathy
CA2553218A1 (en) 2004-01-16 2005-08-04 Mds Pharma Services Fluorescence-based adp detection system
JP4974528B2 (en) * 2004-02-09 2012-07-11 扶桑薬品工業株式会社 Nucleic acid detection method and use thereof
WO2005102345A1 (en) * 2004-03-30 2005-11-03 Alcon, Inc. Use of rho kinase inhibitors in the treatment of hearing loss, tinnitus and improving body balance
US20060115870A1 (en) * 2004-03-30 2006-06-01 Alcon, Inc. High throughput assay for human Rho kinase activity
US20080096238A1 (en) * 2004-03-30 2008-04-24 Alcon, Inc. High throughput assay for human rho kinase activity with enhanced signal-to-noise ratio
US7422868B2 (en) * 2004-07-02 2008-09-09 Promega Corporation Microbial ATP extraction and detection system
ES2325739T3 (en) * 2004-11-18 2009-09-15 Saladax Biomedical Inc. ENZYMATIC MEASUREMENT OF IMATINIB MESILATE.
US7338775B1 (en) 2005-02-09 2008-03-04 Myriad Genetics, Inc. Enzyme assay and use thereof
US20060234323A1 (en) * 2005-04-18 2006-10-19 Cali James J Luminescent ATP detection with extended linearity
US7348159B2 (en) * 2005-04-20 2008-03-25 Boehringer Ingelheim International Gmbh Methods for determining phosphoryltransferase activity using adenosine 5'-triphosphate (ATP)-gamma-S
AU2006306522B9 (en) * 2005-10-21 2011-12-08 Deere & Company Networked multi-role robotic vehicle
CA2642201C (en) 2006-02-13 2014-09-02 Olga Ornatsky Kinase and phosphatase assays conducted by elemental analysis
EP2021793B1 (en) 2006-05-27 2017-04-26 Fluidigm Canada Inc. Polymer backbone element tags
KR100746961B1 (en) 2006-07-31 2007-08-07 한국과학기술연구원 Biosensor for the detection of protein kinase a and kit comprising the same
US20080057531A1 (en) * 2006-08-07 2008-03-06 Oregon Health And Science University Systems, kits, and methods for detecting cariogenic bacteria and assessing risk of dental caries
US7977043B2 (en) * 2006-11-10 2011-07-12 Boehringer Ingelheim International Gmbh Assays useful in determining CD38 inhibition
DE102006059881A1 (en) * 2006-12-19 2008-07-03 Sanofi-Aventis A high-throughput assay for the identification of kinase inhibitors and kinase activators based on the measurement of ATP hydrolysis
CN103983634A (en) * 2008-07-22 2014-08-13 普罗美加公司 ADP detection based luminescent phosphotransferase or ATP hydrolase assay
US20140080161A1 (en) * 2010-09-14 2014-03-20 Research And Diagnostic Systems, Inc. Phosphatase coupled kinase assay
GB201017721D0 (en) * 2010-10-20 2010-12-01 Queen Mary & Westfield College Method
NL2013366B1 (en) 2014-08-26 2016-09-23 Stichting Het Nederlands Kanker Instituut-Antoni van Leeuwenhoek Ziekenhuis Adenylation enzyme inhibitors.
US9677117B2 (en) 2014-10-08 2017-06-13 Promega Corporation Bioluminescent succinate detection assay
JP6522556B2 (en) * 2015-07-06 2019-05-29 富士フイルム株式会社 Blood test kit and analysis method using the same
KR101788096B1 (en) * 2016-04-12 2017-10-19 재단법인 한국파스퇴르연구소 Kit and method for screening antibacterial compound using thiamine monophosphate kinase
JP7414029B2 (en) * 2021-02-26 2024-01-16 横河電機株式会社 Measurement method and measurement system

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Also Published As

Publication number Publication date
US6911319B2 (en) 2005-06-28
JP2004518422A (en) 2004-06-24
EP1341928A2 (en) 2003-09-10
GB0129889D0 (en) 2002-02-06
ES2256165T3 (en) 2006-07-16
WO2002048390A3 (en) 2003-06-26
DE10196608T1 (en) 2003-07-31
DE60115090T2 (en) 2006-07-13
JP3854927B2 (en) 2006-12-06
WO2002048390A2 (en) 2002-06-20
EP1341928B1 (en) 2005-11-16
GB0030727D0 (en) 2001-01-31
DK1341928T3 (en) 2006-03-27
DE60115090D1 (en) 2005-12-22
US20040253658A1 (en) 2004-12-16
US20020172991A1 (en) 2002-11-21
ATE310101T1 (en) 2005-12-15
CA2431274A1 (en) 2002-06-20
CA2431274C (en) 2011-05-17
GB2375171B (en) 2003-03-12
US6599711B2 (en) 2003-07-29
GB2375171A (en) 2002-11-06
US20040077030A1 (en) 2004-04-22
CH694096A5 (en) 2004-07-15

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