AU2002218417A1 - Nucleic acid extraction method and kit - Google Patents

Nucleic acid extraction method and kit

Info

Publication number
AU2002218417A1
AU2002218417A1 AU2002218417A AU1841702A AU2002218417A1 AU 2002218417 A1 AU2002218417 A1 AU 2002218417A1 AU 2002218417 A AU2002218417 A AU 2002218417A AU 1841702 A AU1841702 A AU 1841702A AU 2002218417 A1 AU2002218417 A1 AU 2002218417A1
Authority
AU
Australia
Prior art keywords
kit
nucleic acid
extraction method
acid extraction
nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002218417A
Inventor
Nathan Coombs
Christopher Albert Dale Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GENOVAR DIAGNOSTICS Ltd
Original Assignee
GENOVAR DIAGNOSTICS Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GENOVAR DIAGNOSTICS Ltd filed Critical GENOVAR DIAGNOSTICS Ltd
Publication of AU2002218417A1 publication Critical patent/AU2002218417A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2002218417A 2000-12-05 2001-12-04 Nucleic acid extraction method and kit Abandoned AU2002218417A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0029631.9 2000-12-05
GB0029631A GB2369822A (en) 2000-12-05 2000-12-05 Nucleic acid extraction method and kit
PCT/GB2001/005370 WO2002046463A2 (en) 2000-12-05 2001-12-04 Nucleic acid extraction method and kit

Publications (1)

Publication Number Publication Date
AU2002218417A1 true AU2002218417A1 (en) 2002-06-18

Family

ID=9904473

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002218417A Abandoned AU2002218417A1 (en) 2000-12-05 2001-12-04 Nucleic acid extraction method and kit

Country Status (4)

Country Link
EP (1) EP1417332A2 (en)
AU (1) AU2002218417A1 (en)
GB (1) GB2369822A (en)
WO (1) WO2002046463A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2428941T3 (en) * 2003-03-10 2013-11-12 Expression Pathology, Inc. Liquid tissue preparation from biological samples, tissues and cells histopathologically processed
CN1882688B (en) † 2003-07-25 2014-07-16 安比恩股份有限公司 Methods and compositions for isolating small RNA molecules
EP3061833B1 (en) 2004-09-30 2018-03-14 Epigenomics AG Method for providing dna fragments derived from an archived sample
DE102005023011A1 (en) * 2005-05-19 2006-11-23 Qiagen Gmbh Method of extraction and quantified determination of intact proteins from formalin-fixed tissues, employs heat treatment for their release in absence of proteases
US9243241B2 (en) 2005-05-31 2016-01-26 Life Technologies Corporation Separation and purification of nucleic acid from paraffin-containing samples
JP2008043332A (en) * 2006-08-17 2008-02-28 Panomics Inc Quantitative determination of nucleic acid from tissue slide
EP2395082A1 (en) 2010-06-14 2011-12-14 QIAGEN GmbH Extraction of nucleic acids from wax-embedded samples
WO2012075133A1 (en) * 2010-11-30 2012-06-07 Life Technologies Corporation Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples
JP6278568B2 (en) 2011-12-06 2018-02-14 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング Recovery of aqueous phase containing biomolecules from multiphase mixtures
CN102488710A (en) * 2011-12-14 2012-06-13 吉林大学 Method for extracting thrombin antagonist from pig plasma
CN102680295B (en) * 2012-05-21 2016-01-20 江苏省中医院 A kind of method extracting residual micronucleus acid from HE staining section
JP6201587B2 (en) * 2013-09-30 2017-09-27 凸版印刷株式会社 Nucleic acid extraction method from embedded tissue, embedding agent removal tool, and nucleic acid extraction kit
CN107250376A (en) * 2014-09-26 2017-10-13 加利福尼亚大学董事会 method and system for detecting gene mutation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2153215A1 (en) * 1994-07-06 1996-01-07 Lu Wang Treatment of paraffin embedded tissue for gene analysis
SE9603599D0 (en) * 1996-10-02 1996-10-02 Pharmacia Biotech Ab A method of separating a two phase system
EP0953635B1 (en) * 1998-04-28 2012-08-29 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues

Also Published As

Publication number Publication date
WO2002046463A2 (en) 2002-06-13
GB2369822A (en) 2002-06-12
WO2002046463A3 (en) 2004-02-19
WO2002046463A9 (en) 2003-05-01
GB0029631D0 (en) 2001-01-17
EP1417332A2 (en) 2004-05-12

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