AU2002216431A1 - Method for expression of proteins on spore surface - Google Patents

Method for expression of proteins on spore surface

Info

Publication number
AU2002216431A1
AU2002216431A1 AU2002216431A AU1643102A AU2002216431A1 AU 2002216431 A1 AU2002216431 A1 AU 2002216431A1 AU 2002216431 A AU2002216431 A AU 2002216431A AU 1643102 A AU1643102 A AU 1643102A AU 2002216431 A1 AU2002216431 A1 AU 2002216431A1
Authority
AU
Australia
Prior art keywords
proteins
expression
spore surface
spore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002216431A
Inventor
Soo-Keun Choi
Heung-Chae Jung
June-Hyung Kim
Jae-Gu Pan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GENOFOCUS CO Ltd
Original Assignee
GENOFOCUS CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GENOFOCUS CO Ltd filed Critical GENOFOCUS CO Ltd
Publication of AU2002216431A1 publication Critical patent/AU2002216431A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2002216431A 2000-12-08 2001-12-07 Method for expression of proteins on spore surface Abandoned AU2002216431A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020000074835A KR20020045400A (en) 2000-12-08 2000-12-08 Method for Expression of Proteins on Spore Surface
KR0074835 2000-12-08
PCT/KR2001/002124 WO2002046388A1 (en) 2000-12-08 2001-12-07 Method for expression of proteins on spore surface

Publications (1)

Publication Number Publication Date
AU2002216431A1 true AU2002216431A1 (en) 2002-06-18

Family

ID=19702863

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002216431A Abandoned AU2002216431A1 (en) 2000-12-08 2001-12-07 Method for expression of proteins on spore surface

Country Status (4)

Country Link
US (1) US7582426B2 (en)
KR (2) KR20020045400A (en)
AU (1) AU2002216431A1 (en)
WO (1) WO2002046388A1 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696251B1 (en) * 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6759243B2 (en) * 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
GB0205376D0 (en) * 2002-03-07 2002-04-24 Royal Holloway University Of L Spore germination
KR100518953B1 (en) * 2003-09-19 2005-10-12 주식회사 제노포커스 Method for Whole Surrounding Surface Display of Target Proteins Using Exosporium of Bacillus cereus Group
GB0504940D0 (en) * 2005-03-10 2005-04-20 Secr Defence Vaccine formulation
CA2615057A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
KR100784261B1 (en) * 2006-01-02 2007-12-11 한국과학기술원 Method for Cell Surface Displaying of Target Proteins Using Bacillus anthracis Exosporium
EP2049669A2 (en) * 2006-08-09 2009-04-22 DSMIP Assets B.V. Spore surface displays of bioactive molecules
EP2102339A2 (en) * 2007-01-12 2009-09-23 Sea Lane Biotechnologies,llc. Combinatorial libraries of conformationally constrained polypeptide sequences
US8389263B2 (en) * 2007-09-10 2013-03-05 Sylvia Daunert Spores for the stabilization and on-site application of bacterial whole-cell biosensing systems
KR101583047B1 (en) * 2009-03-30 2016-01-06 한국생명공학연구원 Removal of Heavy Metals Using Spores
KR101244433B1 (en) * 2009-10-19 2013-03-18 한국생명공학연구원 Spore Surface-Immobilized Phytase and Preparation Methods thereof
WO2011071957A1 (en) 2009-12-07 2011-06-16 Sea Lane Biotechnologies, Llc Conjugates comprising an antibody surrogate scaffold with improved pharmacokinetic properties
WO2012001000A1 (en) 2010-06-30 2012-01-05 Dsm Ip Assets B.V. Spore surface display of bioactive molecules
CN102181469A (en) * 2011-03-21 2011-09-14 江苏大学 Recombinant spore for displaying human serum albumin on surface of bacillus subtilis and preparation method thereof
CN103014054A (en) * 2012-11-16 2013-04-03 江南大学 Application of bacillus subtilis spore capsid protein CotZ as molecular carrier in displaying foreign protein on spore surface
CN103409549A (en) * 2013-08-27 2013-11-27 中国农业科学院农业资源与农业区划研究所 Specific primer pair for detecting and identifying PCR of bacillus subtilis
BR122023020886A2 (en) 2014-09-17 2024-01-30 Spogen Biotech Inc METHOD FOR STIMULATING PLANT GROWTH
WO2016140702A1 (en) * 2015-03-03 2016-09-09 The United States Of America, As Represented By The Secretary, Department Of Health & Human Serivces Display platform from bacterial spore coat proteins
CN107338241B (en) * 2016-05-03 2022-02-11 中国科学院深圳先进技术研究院 Method for performing directed evolution on gene promoter
EP3684168A4 (en) 2017-09-20 2021-10-27 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and exosporium fragments for plant health
MX2020005127A (en) 2017-11-16 2020-07-27 Bayer Cropscience Lp Paenibacillus-based endospore display platform, products and methods.
JP2022507370A (en) * 2018-11-15 2022-01-18 バイエル クロップサイエンス エルピー Endogenous spore display platform, products and methods
CN109593695B (en) * 2018-12-06 2022-05-24 齐鲁工业大学 Method for displaying glucose oxidase on surface of bacillus subtilis spore and application
GB201901166D0 (en) * 2019-01-28 2019-03-20 Univ Nottingham Genetic construct and uses thereof
CA3140154A1 (en) * 2019-05-15 2020-11-19 Bayer Cropscience Lp Targeting sequences for paenibacillus-based endospore display platform
KR102259933B1 (en) * 2019-05-16 2021-06-02 국방과학연구소 Method for manufacturing anthrax spore vaccines using spore surface display and anthrax spore vaccines
KR102173586B1 (en) * 2019-05-17 2020-11-03 국방과학연구소 Expression host strains for proteins display on spore surface and method for proteins display on spore surface
WO2022049442A1 (en) * 2020-09-02 2022-03-10 3M Innovative Properties Company Biological indicator comprising genetically-modified test microorganism
US20240254167A1 (en) 2021-02-15 2024-08-01 Livingmed Biotech S.R.L. Genetically modified clostridium strains expressing recombinant antigens and uses thereof
EP4305151A1 (en) 2021-03-11 2024-01-17 Les Biotechnologies Ulysse Inc. Microorganism displaying antigenic protein of the sars-cov2 coronavirus

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5766914A (en) * 1995-01-26 1998-06-16 Michigan State University Method of producing and purifying enzymes
US5800821A (en) * 1995-03-10 1998-09-01 New England Medical Center Hospitals, Inc. Bacterial spores as a heat stable vaccine delivery system
KR100810527B1 (en) * 2001-01-15 2008-03-10 한국생명공학연구원 Method for Surface Display of Proteins on Genetic Carriers

Also Published As

Publication number Publication date
KR20020045400A (en) 2002-06-19
KR20030065534A (en) 2003-08-06
WO2002046388A1 (en) 2002-06-13
US20040171065A1 (en) 2004-09-02
US7582426B2 (en) 2009-09-01
KR100522398B1 (en) 2005-10-18

Similar Documents

Publication Publication Date Title
AU2002216431A1 (en) Method for expression of proteins on spore surface
AU2001234608A1 (en) Methods for analysis of gene expression
AU2001240115A1 (en) Method for the preparation of tetrahydrobenzothiepines
AUPR208000A0 (en) Method of document searching
AU2001241075A1 (en) Method of anticipating interaction between proteins
AU2001256255A1 (en) Method for epoxidizing olefins
AU2002302243A1 (en) Method for determination of co-occurences of attributes
AU2001251414A1 (en) Method of detecting carcinomas
AU2002312859A1 (en) Method for determining gene expression
AU2000279641A1 (en) Method for producing protein hydrolysate
AU2002214286A1 (en) Method of profiling protein
AU2002221740A1 (en) Method for analyzing proteins
AU2001295450A1 (en) Method of polypeptide renaturation
EP1170715A3 (en) Method for surface surveillance
AU2002364749A1 (en) Method for protein expression analysis
AU2001287668A1 (en) Method for the sterilisation of objects
AU2002223548A1 (en) Improved method for the biosynthesis of vitamin e
AU2469300A (en) Method of determining an illuminated surface
AU2002214487A1 (en) Method for inducing apoptiosis
AU2354800A (en) Method of protein removal
AUPQ904100A0 (en) Method of encryption
AU2001230192A1 (en) Method for the enzymatic resolution of the racemates of aminomethyl-aryl-cyclohexanol derivatives
AU2169201A (en) Method for the isolation of hydrophobic proteins
AU2002220757A1 (en) Method for obtaining azaerythromycin
AU2001253092A1 (en) Method for detection of truncated proteins