AU2002212529B2 - Method of measuring platelet activation - Google Patents
Method of measuring platelet activation Download PDFInfo
- Publication number
- AU2002212529B2 AU2002212529B2 AU2002212529A AU2002212529A AU2002212529B2 AU 2002212529 B2 AU2002212529 B2 AU 2002212529B2 AU 2002212529 A AU2002212529 A AU 2002212529A AU 2002212529 A AU2002212529 A AU 2002212529A AU 2002212529 B2 AU2002212529 B2 AU 2002212529B2
- Authority
- AU
- Australia
- Prior art keywords
- platelet
- blood
- edta
- anticoagulated
- ctad
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 230000010118 platelet activation Effects 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims description 31
- 210000001772 blood platelet Anatomy 0.000 claims abstract description 192
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 114
- 239000003146 anticoagulant agent Substances 0.000 claims abstract description 64
- 229940127219 anticoagulant drug Drugs 0.000 claims abstract description 63
- 239000000203 mixture Substances 0.000 claims abstract description 36
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims abstract description 34
- ZFXYFBGIUFBOJW-UHFFFAOYSA-N theophylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1NC=N2 ZFXYFBGIUFBOJW-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims abstract description 17
- 229960005305 adenosine Drugs 0.000 claims abstract description 17
- IZEKFCXSFNUWAM-UHFFFAOYSA-N dipyridamole Chemical compound C=12N=C(N(CCO)CCO)N=C(N3CCCCC3)C2=NC(N(CCO)CCO)=NC=1N1CCCCC1 IZEKFCXSFNUWAM-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229960002768 dipyridamole Drugs 0.000 claims abstract description 17
- 229960000278 theophylline Drugs 0.000 claims abstract description 16
- 239000005557 antagonist Substances 0.000 claims abstract description 9
- 210000004369 blood Anatomy 0.000 claims description 183
- 239000008280 blood Substances 0.000 claims description 174
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 18
- 230000004913 activation Effects 0.000 claims description 18
- 238000005259 measurement Methods 0.000 claims description 18
- 239000000106 platelet aggregation inhibitor Substances 0.000 claims description 9
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims description 8
- BIXYYZIIJIXVFW-UUOKFMHZSA-N (2R,3R,4S,5R)-2-(6-amino-2-chloro-9-purinyl)-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound C1=NC=2C(N)=NC(Cl)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O BIXYYZIIJIXVFW-UUOKFMHZSA-N 0.000 claims description 7
- 229960001484 edetic acid Drugs 0.000 description 108
- 101000622137 Homo sapiens P-selectin Proteins 0.000 description 46
- 102100023472 P-selectin Human genes 0.000 description 46
- 238000004458 analytical method Methods 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 108090000190 Thrombin Proteins 0.000 description 10
- 229960004072 thrombin Drugs 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 9
- 239000011575 calcium Substances 0.000 description 9
- 230000008859 change Effects 0.000 description 9
- 230000003287 optical effect Effects 0.000 description 8
- XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 description 7
- XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 description 7
- 238000000684 flow cytometry Methods 0.000 description 7
- 210000000440 neutrophil Anatomy 0.000 description 7
- 239000001509 sodium citrate Substances 0.000 description 7
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 6
- 229910052791 calcium Inorganic materials 0.000 description 6
- 230000003247 decreasing effect Effects 0.000 description 6
- 230000001419 dependent effect Effects 0.000 description 6
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 6
- 101001071312 Homo sapiens Platelet glycoprotein IX Proteins 0.000 description 5
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 5
- 102100036851 Platelet glycoprotein IX Human genes 0.000 description 5
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 5
- 238000007792 addition Methods 0.000 description 5
- 238000010790 dilution Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 208000007536 Thrombosis Diseases 0.000 description 4
- 238000004820 blood count Methods 0.000 description 4
- 239000002738 chelating agent Substances 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000011835 investigation Methods 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000012552 review Methods 0.000 description 4
- IJRKANNOPXMZSG-SSPAHAAFSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanal Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O.OC(=O)CC(O)(C(O)=O)CC(O)=O IJRKANNOPXMZSG-SSPAHAAFSA-N 0.000 description 3
- 241000220317 Rosa Species 0.000 description 3
- 238000000149 argon plasma sintering Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229940001468 citrate Drugs 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000007427 paired t-test Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000001681 protective effect Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 2
- 241001460678 Napo <wasp> Species 0.000 description 2
- 108091006629 SLC13A2 Proteins 0.000 description 2
- 229960001138 acetylsalicylic acid Drugs 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 230000010100 anticoagulation Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000002489 hematologic effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000013214 routine measurement Methods 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 229960003766 thrombin (human) Drugs 0.000 description 2
- 230000036962 time dependent Effects 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 206010003178 Arterial thrombosis Diseases 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 description 1
- 108090001050 Phosphoric Diester Hydrolases Proteins 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 108010035030 Platelet Membrane Glycoprotein IIb Proteins 0.000 description 1
- 108010010336 Platelet Membrane Glycoproteins Proteins 0.000 description 1
- 102000015795 Platelet Membrane Glycoproteins Human genes 0.000 description 1
- 102000004211 Platelet factor 4 Human genes 0.000 description 1
- 108090000778 Platelet factor 4 Proteins 0.000 description 1
- 238000010165 Scheffé test Methods 0.000 description 1
- IVOMOUWHDPKRLL-UHFFFAOYSA-N UNPD107823 Natural products O1C2COP(O)(=O)OC2C(O)C1N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-UHFFFAOYSA-N 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical class N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000702 anti-platelet effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 229940095074 cyclic amp Drugs 0.000 description 1
- 238000004163 cytometry Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 108010047482 ectoATPase Proteins 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- 230000020764 fibrinolysis Effects 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 238000005534 hematocrit Methods 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011545 laboratory measurement Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000006740 morphological transformation Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000018127 platelet degranulation Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 210000001243 pseudopodia Anatomy 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007430 reference method Methods 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 206010043554 thrombocytopenia Diseases 0.000 description 1
- 230000001732 thrombotic effect Effects 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2525—Stabilizing or preserving
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Ecology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0027309.4A GB0027309D0 (en) | 2000-11-08 | 2000-11-08 | Method of measuring measuring platlet activation |
| GB0027309.4 | 2000-11-08 | ||
| PCT/GB2001/004946 WO2002039124A1 (en) | 2000-11-08 | 2001-11-08 | Method of measuring platelet activation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2002212529A1 AU2002212529A1 (en) | 2002-07-25 |
| AU2002212529B2 true AU2002212529B2 (en) | 2006-08-10 |
Family
ID=9902807
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2002212529A Ceased AU2002212529B2 (en) | 2000-11-08 | 2001-11-08 | Method of measuring platelet activation |
| AU1252902A Pending AU1252902A (en) | 2000-11-08 | 2001-11-08 | Method of measuring platelet activation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU1252902A Pending AU1252902A (en) | 2000-11-08 | 2001-11-08 | Method of measuring platelet activation |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7011938B2 (enExample) |
| EP (1) | EP1332373B1 (enExample) |
| JP (1) | JP2004513369A (enExample) |
| AT (1) | ATE522808T1 (enExample) |
| AU (2) | AU2002212529B2 (enExample) |
| CA (1) | CA2428224C (enExample) |
| ES (1) | ES2372810T3 (enExample) |
| GB (1) | GB0027309D0 (enExample) |
| WO (1) | WO2002039124A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2392724A (en) * | 2002-09-06 | 2004-03-10 | Ian Stoakes | Methods of and apparatus for blood analysis |
| EP1814563A4 (en) * | 2004-07-26 | 2008-04-16 | Bayer Healthcare Llc | PLATTOCK ACTIVATING MARKERS AS PREDICATORS OF ILLNESSES AND THE RESPONSE TO TREATMENTS AND MONITORING THERAPEUTIC PROGRESS |
| US7811549B2 (en) | 2006-07-05 | 2010-10-12 | Adenobio N.V. | Methods, compositions, unit dosage forms, and kits for pharmacologic stress testing with reduced side effects |
| US8021854B2 (en) * | 2007-10-05 | 2011-09-20 | Attila Mady | Method of treating vascular disease |
| US8835104B2 (en) * | 2007-12-20 | 2014-09-16 | Fenwal, Inc. | Medium and methods for the storage of platelets |
| WO2009092516A2 (en) * | 2008-01-22 | 2009-07-30 | Adenobio N.V. | Methods, compositions, unit dosage forms, and kits for pharmacologic stress testing with reduced side effects |
| WO2011140241A2 (en) | 2010-05-04 | 2011-11-10 | The General Hospital Corporation | Methods and compositions for preserving tissues and organs |
| WO2012139017A1 (en) | 2011-04-07 | 2012-10-11 | Fenwal, Inc. | Automated methods and systems for providing platelet concentrates with reduced residual plasma volumes and storage media for such platelet concentrates |
| EP3054936B1 (en) | 2013-10-10 | 2023-10-18 | Eastern Virginia Medical School | 4-((2-hydroxy-3-methoxybenzyl)amino) benzenesulfonamide derivatives as 12-lipoxygenase inhibitors |
| EP3698628A1 (en) | 2014-08-07 | 2020-08-26 | The General Hospital Corporation | Stabilization of whole blood samples |
| US20180017584A1 (en) | 2016-07-12 | 2018-01-18 | Emulate Inc. | Additive channels |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08220094A (ja) * | 1995-02-08 | 1996-08-30 | Dai Ichi Pure Chem Co Ltd | 血漿中ｇｍｐ−１４０の測定法 |
| FR2731277A1 (fr) * | 1995-03-02 | 1996-09-06 | Benveniste Jacques | Procedes et appareil pour faciliter la purification, le comptage et la mesure de l'activation des leucocytes sanguins |
| US5817519A (en) * | 1995-12-28 | 1998-10-06 | Bayer Corporation | Automated method and device for identifying and quantifying platelets and for determining platelet activation state using whole blood samples |
| US6309888B1 (en) * | 1998-09-04 | 2001-10-30 | Leuven Research & Development Vzw | Detection and determination of the stages of coronary artery disease |
-
2000
- 2000-11-08 GB GBGB0027309.4A patent/GB0027309D0/en not_active Ceased
-
2001
- 2001-11-08 AT AT01980741T patent/ATE522808T1/de not_active IP Right Cessation
- 2001-11-08 AU AU2002212529A patent/AU2002212529B2/en not_active Ceased
- 2001-11-08 AU AU1252902A patent/AU1252902A/xx active Pending
- 2001-11-08 WO PCT/GB2001/004946 patent/WO2002039124A1/en not_active Ceased
- 2001-11-08 EP EP01980741A patent/EP1332373B1/en not_active Expired - Lifetime
- 2001-11-08 ES ES01980741T patent/ES2372810T3/es not_active Expired - Lifetime
- 2001-11-08 CA CA2428224A patent/CA2428224C/en not_active Expired - Fee Related
- 2001-11-08 JP JP2002541398A patent/JP2004513369A/ja active Pending
- 2001-11-08 US US10/415,336 patent/US7011938B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2372810T3 (es) | 2012-01-26 |
| WO2002039124A1 (en) | 2002-05-16 |
| EP1332373A1 (en) | 2003-08-06 |
| US20040038997A1 (en) | 2004-02-26 |
| AU1252902A (en) | 2002-05-21 |
| CA2428224A1 (en) | 2002-05-16 |
| GB0027309D0 (en) | 2000-12-27 |
| CA2428224C (en) | 2010-04-27 |
| US7011938B2 (en) | 2006-03-14 |
| EP1332373B1 (en) | 2011-08-31 |
| ATE522808T1 (de) | 2011-09-15 |
| JP2004513369A (ja) | 2004-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Macey et al. | Evaluation of the anticoagulants EDTA and citrate, theophylline, adenosine, and dipyridamole (CTAD) for assessing platelet activation on the ADVIA 120 hematology system | |
| Macey et al. | Use of mean platelet component to measure platelet activation on the ADVIA 120 haematology system | |
| Harris et al. | The ADVIA 2120 hematology system: flow cytometry-based analysis of blood and body fluids in the routine hematology laboratory | |
| JP3704654B2 (ja) | 全血サンプルを使用して血小板を同定および定量し、血小板の活性化状態を測定する高感度で正確かつ精密な自動化方法 | |
| Ahnadi et al. | Assessment of platelet activation in several different anticoagulants by the Advia 120 Hematology System, fluorescence flow cytometry, and electron microscopy | |
| Zelmanovic et al. | Automated analysis of feline platelets in whole blood, including platelet count, mean platelet volume, and activation state | |
| JP2565844B2 (ja) | 細胞溶解処理条件下で異種細胞集団を正確に計数し，感受性に関する格付けを行う方法 | |
| EP1004880B1 (en) | Erythroblast diagnostic flow-cytometry method and reagents | |
| AU669692B2 (en) | Suspension media for hematological composition and method for its use | |
| Kratz et al. | Effects of marathon running on platelet activation markers: direct evidence for in vivo platelet activation | |
| US5858790A (en) | Hematology reference control and method of preparation | |
| Ballas et al. | Red cell membrane and cation deficiency in Rh null syndrome | |
| Minter et al. | Platelet volume: density relationships in normal and acutely bled dogs | |
| AU2002212529B2 (en) | Method of measuring platelet activation | |
| Krueger et al. | Immunophenotypic analysis of platelets | |
| US7449337B2 (en) | Lytic reagent and method for leukocytes differential in whole blood | |
| AU2002212529A1 (en) | Method of measuring platelet activation | |
| Brittin et al. | Stability of blood in commonly used anticoagulants: use of refrigerated blood for quality control of the Coulter Counter Model S | |
| JP2004513369A5 (enExample) | ||
| Patton et al. | A study of changes in red cell volume and haemoglobin concentration during phlebotomy induced iron deficiency and iron repletion using the Technicon H1 | |
| EP2291652B1 (en) | Stabilisation of blood cell conjugates | |
| Nemec et al. | The effect of high anticoagulant k3-edta concentration on complete blood count and white blood cell differential counts in healthy beagle dogs | |
| Solanki et al. | Spurious red blood cell parameters due to serum cold agglutinins: Observations on Ortho ELT-8® Cell Counter | |
| Reininger | Primary haemostasis and its assessment by laboratory tests | |
| Zandecki et al. | Spurious counts and spurious results on hematology analyzers: white blood cells, red blood cells, hemoglobin, red cell indices, and reticulocytes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |