AU2001293727A1 - Moving droplet diagnostic assay - Google Patents

Moving droplet diagnostic assay

Info

Publication number
AU2001293727A1
AU2001293727A1 AU2001293727A AU9372701A AU2001293727A1 AU 2001293727 A1 AU2001293727 A1 AU 2001293727A1 AU 2001293727 A AU2001293727 A AU 2001293727A AU 9372701 A AU9372701 A AU 9372701A AU 2001293727 A1 AU2001293727 A1 AU 2001293727A1
Authority
AU
Australia
Prior art keywords
droplet
analyte
solid phase
binding agent
applying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001293727A
Inventor
Thomas Augustinus Maria Beumer
Hendrik Sibolt Van Damme
Petrus Franciscus Hendrikus M. Verheijden
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux BV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Publication of AU2001293727A1 publication Critical patent/AU2001293727A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2525—Stabilizing or preserving

Abstract

The present invention concerns a method for the detection of an analyte in a droplet of fluid comprising the steps of providing a solid phase with a hydrophobic surface comprising at least one capture zone on which at least one binding agent with affinity for the analyte is immobilized, applying said droplet to the surface of said solid phase, applying a force that makes the droplet travel along the surface of said solid phase along a predetermined path thereby allowing the droplet to repeatedly contact said binding agent on the capture zone, applying conditions wherein said analyte is allowed to bind to said binding agent and detecting a complex of analyte and binding reagent at the position of the capture zone. The effect of a moving droplet is that the reactants in the droplet are well mixed which eliminates the risk of diffusion limitation. Such advantageous mixing is not obtained with conventional assays in which the surface is continuously exposed to the liquid. The use of a droplet eliminates the need for external mixing and also circumvents the need to dilute the sample in order to obtain sufficient volume to wet a large surface area.
AU2001293727A 2000-08-10 2001-08-06 Moving droplet diagnostic assay Abandoned AU2001293727A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00202811 2000-08-10
EP00202811 2000-08-10
PCT/EP2001/009073 WO2002012895A1 (en) 2000-08-10 2001-08-06 Moving droplet diagnostic assay

Publications (1)

Publication Number Publication Date
AU2001293727A1 true AU2001293727A1 (en) 2002-02-18

Family

ID=8171900

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001293727A Abandoned AU2001293727A1 (en) 2000-08-10 2001-08-06 Moving droplet diagnostic assay

Country Status (6)

Country Link
US (1) US8586349B2 (en)
EP (1) EP1309865B1 (en)
AT (1) ATE433574T1 (en)
AU (1) AU2001293727A1 (en)
DE (1) DE60138953D1 (en)
WO (1) WO2002012895A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8361782B2 (en) * 2007-05-02 2013-01-29 Siemens Healthcare Diagnostics, Inc. Piezo dispensing of a diagnostic liquid into microfluidic devices
CN101688876A (en) * 2007-05-02 2010-03-31 西门子医疗保健诊断公司 Piezo dispensing of a diagnostic liquid onto a reagent surface

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL7807532A (en) 1978-07-13 1980-01-15 Akzo Nv METAL IMMUNO TEST.
NL8000173A (en) 1980-01-11 1981-08-03 Akzo Nv USE OF WATER-DISPERSIBLE HYDROPHOBIC DYES AS LABELS IN IMMUNOCHEMICAL TESTS.
US4703017C1 (en) 1984-02-14 2001-12-04 Becton Dickinson Co Solid phase assay with visual readout
US4632901A (en) 1984-05-11 1986-12-30 Hybritech Incorporated Method and apparatus for immunoassays
US4920046A (en) 1987-02-20 1990-04-24 Becton, Dickinson And Company Process, test device, and test kit for a rapid assay having a visible readout
CA1303983C (en) 1987-03-27 1992-06-23 Robert W. Rosenstein Solid phase assay
DE3887771C5 (en) 1987-04-27 2009-06-04 Inverness Medical Switzerland Gmbh Immunoassays and devices therefor.
US5726026A (en) * 1992-05-01 1998-03-10 Trustees Of The University Of Pennsylvania Mesoscale sample preparation device and systems for determination and processing of analytes
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
US5637469A (en) * 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
WO1996014434A1 (en) * 1994-11-07 1996-05-17 The Institute Of Physical And Chemical Research Method of dna sequencing
US5773234A (en) * 1995-08-07 1998-06-30 Quidel Corporation Method and device for chlamydia detection
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
DE19621488C2 (en) * 1996-05-29 1998-05-14 Gerlach Gerald F Prof Dr Diagnostic procedure for the detection of subclinical mammals suffering from paratuberculosis
US6074827A (en) * 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US5842787A (en) * 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
WO1999053093A1 (en) * 1998-04-08 1999-10-21 Universität Heidelberg Method for carrying out reactions between at least two reaction partners in aqueous reaction mixtures
US6180417B1 (en) * 1999-04-22 2001-01-30 Bayer Corporation Immunochromatographic assay

Also Published As

Publication number Publication date
ATE433574T1 (en) 2009-06-15
EP1309865B1 (en) 2009-06-10
US20040043421A1 (en) 2004-03-04
WO2002012895A1 (en) 2002-02-14
US8586349B2 (en) 2013-11-19
EP1309865A1 (en) 2003-05-14
DE60138953D1 (en) 2009-07-23

Similar Documents

Publication Publication Date Title
JP4869602B2 (en) Method and apparatus for dividing a specimen into multiple channels of a microfluidic device
Choi et al. An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities
Hervás et al. Electrochemical immunosensing on board microfluidic chip platforms
JP4351539B2 (en) Method and apparatus for accurately moving and manipulating fluid by centrifugal force and / or capillary force
Hervas et al. Integrated electrokinetic magnetic bead-based electrochemical immunoassay on microfluidic chips for reliable control of permitted levels of zearalenone in infant foods
EP1033575A3 (en) Device for carrying out lateral-flow assays involving more than one analyte
JP2000516343A (en) Capillary electrophoresis enzyme immunoassay
CA2250242A1 (en) Quantitative immunochromatographic assays
CA2252912A1 (en) Assay method, kit and assay device
JP2007502979A (en) Mixing in microfluidic devices
JP2007502979A5 (en)
WO2005029073A3 (en) Chromatographic assay device and methods
WO1990008319A1 (en) Threshold ligand-receptor assay
WO2000058730A8 (en) Method and device for detecting analytes in fluids
CA2081559A1 (en) Determination of analytes in biological fluids in the presence of substances interfering with assays therefor
KR940005955A (en) Solid phase analysis
EP1046913A3 (en) Immunochromatographic assay
WO2003087822A3 (en) Sensitive immunochromatographic assay
US9778271B2 (en) Ratiometric immunoassay method and blood testing device
WO2000072020A8 (en) Microscale diffusion immunoassay
Tu et al. Paper microfluidic device with a horizontal motion valve and a localized delay for automatic control of a multistep assay
JP2011530703A (en) Analytical strip
AU2001293727A1 (en) Moving droplet diagnostic assay
CA2110049A1 (en) Devices for performing ion-capture binding assays
JP5162031B2 (en) Liquid inspection method