WO1992022797A3 - Assay for the detection of specific ligands - Google Patents

Assay for the detection of specific ligands Download PDF

Info

Publication number
WO1992022797A3
WO1992022797A3 PCT/US1992/003680 US9203680W WO9222797A3 WO 1992022797 A3 WO1992022797 A3 WO 1992022797A3 US 9203680 W US9203680 W US 9203680W WO 9222797 A3 WO9222797 A3 WO 9222797A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
zone
antigen
conjugate
present
Prior art date
Application number
PCT/US1992/003680
Other languages
French (fr)
Other versions
WO1992022797A2 (en
Inventor
Sinfu Tzeng
Dou-Mei Wang
Original Assignee
Pacific Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biotech Inc filed Critical Pacific Biotech Inc
Priority to KR1019930703834A priority Critical patent/KR940701542A/en
Priority to JP5500852A priority patent/JPH06508689A/en
Publication of WO1992022797A2 publication Critical patent/WO1992022797A2/en
Publication of WO1992022797A3 publication Critical patent/WO1992022797A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pyrane Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

The invention disclosed herein relates to the field of one-step assays and particle agglutination tests. In one embodiment of the present invention, there is disclosed an analytical device for the detection or determination of an analyte antibody in a bodily fluid comprising a layer of a plurality of substantially planar zones adjacent one another and in absorbent contact with one another, the layer including a sample application zone, a conjugate zone containing antigen bound to mobile particles, and a detection zone containing immobilized antigen, wherein the antigen is the same in both the conjugate and detection zones and is an antigen that binds with the analyte antibody, the liquid sample is capable of moving from the sample application zone through the conjugate zone and on to the detection zone, and if the analyte antibody is present in the sample it is detected in the detection zone. The present invention also discloses methods of detecting various analytes, particularly analyte antibodies, including specific immunoglobulins. The invention further provides improved methods of preparing and using one-step assays, as well as improved methods of preparing coated particles for use in diagnostic assays.
PCT/US1992/003680 1991-06-13 1992-05-05 Assay for the detection of specific ligands WO1992022797A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1019930703834A KR940701542A (en) 1991-06-13 1992-05-05 Assays for Detecting Specific Ligands
JP5500852A JPH06508689A (en) 1991-06-13 1992-05-05 Assays for detecting specific ligands

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US71540791A 1991-06-13 1991-06-13
US715,407 1991-06-13
US71511991A 1991-06-14 1991-06-14
US715,119 1991-06-14

Publications (2)

Publication Number Publication Date
WO1992022797A2 WO1992022797A2 (en) 1992-12-23
WO1992022797A3 true WO1992022797A3 (en) 1993-04-01

Family

ID=27109275

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1992/003680 WO1992022797A2 (en) 1991-06-13 1992-05-05 Assay for the detection of specific ligands

Country Status (6)

Country Link
EP (1) EP0588958A1 (en)
JP (1) JPH06508689A (en)
KR (1) KR940701542A (en)
AU (1) AU2247192A (en)
CA (1) CA2110920A1 (en)
WO (1) WO1992022797A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6653066B1 (en) * 1994-06-17 2003-11-25 Trinity Biotech Device and method for detecting polyvalent substances
GB2300914B (en) * 1995-04-28 1998-04-29 Tepnel Medical Ltd Analytical device
US5753517A (en) * 1996-03-29 1998-05-19 University Of British Columbia Quantitative immunochromatographic assays
AU757629B2 (en) * 1998-04-14 2003-02-27 Otsuka Pharmaceutical Co., Ltd. Methods for assaying antibody and device for assaying antibody
EP0973034A1 (en) * 1998-07-16 2000-01-19 Microbe Scope AG Immunoassays and devices therefor
GB9823397D0 (en) * 1998-10-27 1998-12-23 Rsr Ltd Assays for thyroid autoantibodies
US7410808B1 (en) 2003-12-08 2008-08-12 Charm Sciences, Inc. Method and assay for detection of residues
JP5414424B2 (en) * 2009-08-31 2014-02-12 シスメックス株式会社 Method for detecting target substance and chromatographic test kit for detecting target substance
DK2566949T3 (en) 2010-05-06 2023-05-22 Charm Sciences Inc Reader incubator device
KR101353930B1 (en) 2012-02-20 2014-01-27 주식회사 나노엔텍 A Novel Method for Detecting an Antigen and Apparatus Using It
JP2013185858A (en) * 2012-03-06 2013-09-19 Gc Corp Examination method and measurement instrument for periodontal disease pathogenic bacteria using outer membrane vesicles

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0189925A2 (en) * 1985-01-30 1986-08-06 Genetic Diagnostics Corporation Self-timed immunoassay device
WO1986004683A1 (en) * 1985-02-08 1986-08-14 Boehringer Biochemia Robin S.P.A. Determination of clinical parameters by enzyme immunoprocess
EP0252641A1 (en) * 1986-06-26 1988-01-13 BioWhittaker, Inc. Diagnosis of lyme disease
EP0299428A2 (en) * 1987-07-13 1989-01-18 Abbott Laboratories Process for immunochromatography with colloidal particles
EP0313986A2 (en) * 1987-10-30 1989-05-03 Abbott Laboratories Immunoassays using antigens produced in heterologous organisms
EP0348159A2 (en) * 1988-06-20 1989-12-27 University Of Guelph Rapid stick test for detecion of antibodies in a specimen
WO1990004411A1 (en) * 1988-10-24 1990-05-03 Symbicom Aktiebolag Immunogenically active fractions of borrelia burgdorferi
EP0421294A2 (en) * 1989-10-05 1991-04-10 Abbott Laboratories Improved self-performing immunochromatographic device

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0189925A2 (en) * 1985-01-30 1986-08-06 Genetic Diagnostics Corporation Self-timed immunoassay device
WO1986004683A1 (en) * 1985-02-08 1986-08-14 Boehringer Biochemia Robin S.P.A. Determination of clinical parameters by enzyme immunoprocess
EP0252641A1 (en) * 1986-06-26 1988-01-13 BioWhittaker, Inc. Diagnosis of lyme disease
EP0299428A2 (en) * 1987-07-13 1989-01-18 Abbott Laboratories Process for immunochromatography with colloidal particles
EP0313986A2 (en) * 1987-10-30 1989-05-03 Abbott Laboratories Immunoassays using antigens produced in heterologous organisms
EP0348159A2 (en) * 1988-06-20 1989-12-27 University Of Guelph Rapid stick test for detecion of antibodies in a specimen
WO1990004411A1 (en) * 1988-10-24 1990-05-03 Symbicom Aktiebolag Immunogenically active fractions of borrelia burgdorferi
EP0421294A2 (en) * 1989-10-05 1991-04-10 Abbott Laboratories Improved self-performing immunochromatographic device

Also Published As

Publication number Publication date
AU2247192A (en) 1993-01-12
JPH06508689A (en) 1994-09-29
CA2110920A1 (en) 1992-12-23
EP0588958A1 (en) 1994-03-30
WO1992022797A2 (en) 1992-12-23
KR940701542A (en) 1994-05-28

Similar Documents

Publication Publication Date Title
US9250236B2 (en) Method to increase specificity and/or accuracy of lateral flow immunoassays
JP2619236B2 (en) Method for deeply knowing an analyte in a liquid sample and an analytical tool used in the method
EP0630475B1 (en) Red blood cell separation means for specific binding assays
EP0465266B1 (en) Complementary visual signal immunoassay
AU720394B2 (en) Opposable-element assay device employing conductive barrier
CA2330100C (en) Ligand binding assay and kit with a separation zone for disturbing analytes
EP0303110B1 (en) Immunodiagnostic device and method
EP0447154B1 (en) Device for ligand-receptor methods
US20060240569A1 (en) Semi-quantitative immunochromatographic device
AU2020201874B2 (en) Immunoassay utilizing trapping conjugate
AU2002334550B2 (en) Multi-analyte assay device with multi-spot detection zone
JPH08501627A (en) Self-verification efficacy test device
KR830009486A (en) Analysis method and apparatus of substance
US6689317B1 (en) Immunoassay apparatus for diagnosis
CA2128320A1 (en) Calibration reagents for semi-quantitative binding assays and devices
WO1998039657A1 (en) Quantitative lateral flow assays and devices
AU2002334550A1 (en) Multi-analyte assay device with multi-spot detection zone
US20030073121A1 (en) Multi-analyte assay device with multi-spot detection zone
WO1992022797A3 (en) Assay for the detection of specific ligands
CA2349274A1 (en) Assay for anti transglutaminase antibodies
US20030027222A1 (en) Test device for detecting human blood and method of use
WO1998023958A1 (en) Competitive lateral flow, specific binding chromatographic assay devices, kits, and methods
AU2003200222B2 (en) Ligand binding assay and kit with a separation zone for disturbing analytes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA FI JP KR NO

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA FI JP KR NO

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IT LU MC NL SE

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WR Later publication of a revised version of an international search report
WWE Wipo information: entry into national phase

Ref document number: 2110920

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 1992914168

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 1992914168

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 1992914168

Country of ref document: EP