AU2001283934A1 - Method for the examination of cells in a culture medium - Google Patents

Method for the examination of cells in a culture medium

Info

Publication number
AU2001283934A1
AU2001283934A1 AU2001283934A AU8393401A AU2001283934A1 AU 2001283934 A1 AU2001283934 A1 AU 2001283934A1 AU 2001283934 A AU2001283934 A AU 2001283934A AU 8393401 A AU8393401 A AU 8393401A AU 2001283934 A1 AU2001283934 A1 AU 2001283934A1
Authority
AU
Australia
Prior art keywords
cells
windows
examination
separation
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001283934A
Inventor
Christoph. Bittner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
innovatis AG
Original Assignee
INNOVATIS GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INNOVATIS GmbH filed Critical INNOVATIS GmbH
Publication of AU2001283934A1 publication Critical patent/AU2001283934A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1493—Particle size

Abstract

The invention relates to a method for the examination of cells ( 20, 38 ) in a culture medium, in particular for in-situ microscopy in a bio-reactor, whereby cells in a sample volume, the depth (d) of which is defined by windows ( 14, 16 ) in the direction of the optical axis of the microscope ( 18 ), are microscopically imaged and are automatically recorded and processed by means of an image processing system ( 30 ). Said method is characterized in that the depth (d) of the sample volume ( 12 ) is adjusted to the size of the cells ( 20, 38 ) by successively reducing the separation (d) of the windows while the image size (G) of the cells is simultaneously verified by the image processing system ( 30 ) such that a separation value (D) is determined at which the image size (G) of the cells begins to grow, thus corresponding to flattening caused by the contact pressure of the windows ( 14, 16 ), and that the separation (d) of the windows ( 14, 16 ) is set to said separation value (D) for the examination.
AU2001283934A 2000-07-10 2001-07-07 Method for the examination of cells in a culture medium Abandoned AU2001283934A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10033268 2000-07-10
DE10033268A DE10033268C2 (en) 2000-07-10 2000-07-10 Method for examining cells in a culture fluid
PCT/EP2001/007814 WO2002004924A1 (en) 2000-07-10 2001-07-07 Method for the examination of cells in a culture medium

Publications (1)

Publication Number Publication Date
AU2001283934A1 true AU2001283934A1 (en) 2002-01-21

Family

ID=7648266

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001283934A Abandoned AU2001283934A1 (en) 2000-07-10 2001-07-07 Method for the examination of cells in a culture medium

Country Status (8)

Country Link
US (1) US7094562B2 (en)
EP (1) EP1299707B1 (en)
JP (1) JP2004503223A (en)
CN (1) CN1187437C (en)
AT (1) ATE286247T1 (en)
AU (1) AU2001283934A1 (en)
DE (2) DE10033268C2 (en)
WO (1) WO2002004924A1 (en)

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CN102016578B (en) 2008-03-21 2014-10-01 艾博特健康公司 Method and apparatus for determining the hematocrit of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
CA2719004C (en) 2008-03-21 2013-06-18 Abbott Point Of Care, Inc. Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample
US8081303B2 (en) 2008-03-21 2011-12-20 Abbott Point Of Care, Inc. Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
CA2718995C (en) * 2008-03-21 2014-08-19 Abbott Point Of Care, Inc. Method and apparatus for determining red blood cell indices of a blood sample utilizing the intrinsic pigmentation of hemoglobin contained within the red blood cells
US8221985B2 (en) 2008-04-02 2012-07-17 Abbott Point Of Care, Inc. Self-calibrating gradient dilution in a constituent assay and gradient dilution apparatus performed in a thin film sample
CA2720072C (en) * 2008-04-09 2013-11-19 Abbott Point Of Care, Inc. Method of detecting very low levels of analyte within a thin film fluid sample contained in a thin thickness chamber
EP2269033B1 (en) * 2008-04-09 2016-09-21 Abbott Point Of Care, Inc. Method for measuring the area of a sample disposed within an analysis chamber
DE102009012227B4 (en) * 2009-03-07 2011-09-01 Juri Charlot Containers for holding nutrient solutions or culture media
US20100255605A1 (en) * 2009-04-02 2010-10-07 Abbott Point Of Care, Inc. Method and device for transferring biologic fluid samples
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WO2011082342A1 (en) * 2009-12-31 2011-07-07 Abbott Point Of Care, Inc. Method and apparatus for determining mean cell volume of red blood cells
WO2011116305A1 (en) 2010-03-18 2011-09-22 Abbott Point Of Care, Inc. Method and apparatus for optically determining at least one hemoglobin related parameter of a whole blood sample
US9523682B2 (en) 2011-11-16 2016-12-20 Becton, Dickinson And Company Methods and systems for detecting an analyte in a sample
CN104540378B (en) 2012-06-18 2017-05-17 绿玛瑙有限公司 A compact apparatus for continuous production of a product substance from a starter material grown in aquaculture conditions
CN104755925B (en) 2013-01-11 2017-06-23 贝克顿·迪金森公司 The point-of-care of low cost determines device
US10502666B2 (en) 2013-02-06 2019-12-10 Alentic Microscience Inc. Sample processing improvements for quantitative microscopy
CN105765440B (en) 2013-06-26 2020-08-18 阿兰蒂克微科学股份有限公司 Sample processing improved device and method for microscopy
US9063080B2 (en) 2013-07-26 2015-06-23 Ecolab Usa Inc. Method of deposition monitoring
CN106029863A (en) 2013-11-06 2016-10-12 贝克顿·迪金森公司 Microfluidic devices, and methods of making and using the same
JP6518245B2 (en) 2013-11-13 2019-05-22 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Optical imaging system and method using the same
US10039244B2 (en) 2014-03-04 2018-08-07 Greenonyx Ltd Systems and methods for cultivating and distributing aquatic organisms
US9347082B2 (en) * 2014-08-28 2016-05-24 Omnivision Technologies, Inc. Automated cell growth/migration detection system and associated methods
EP3206581B1 (en) 2014-10-14 2018-09-19 Becton, Dickinson and Company Blood sample management using open cell foam
PL3094252T3 (en) 2014-10-14 2022-02-21 Becton, Dickinson And Company Blood sample management using open cell foam
EP3381559B1 (en) 2015-03-10 2024-02-28 Becton, Dickinson and Company Biological fluid micro-sample management device
CA2996863C (en) 2015-09-01 2021-04-13 Becton, Dickinson And Company Depth filtration device for separating specimen phases
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EP3639081A4 (en) 2017-06-13 2021-03-03 The Trustees Of Princeton University Fully automatic, template -free particle picking for electron microscopy
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Also Published As

Publication number Publication date
US20040048330A1 (en) 2004-03-11
DE10033268A1 (en) 2002-02-07
EP1299707A1 (en) 2003-04-09
ATE286247T1 (en) 2005-01-15
CN1187437C (en) 2005-02-02
EP1299707B1 (en) 2004-12-29
JP2004503223A (en) 2004-02-05
DE10033268C2 (en) 2002-08-08
DE50104979D1 (en) 2005-02-03
CN1441899A (en) 2003-09-10
WO2002004924A1 (en) 2002-01-17
US7094562B2 (en) 2006-08-22

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