AU2001278683A1 - Novel carbonyl reductase, gene thereof and method of using the same - Google Patents

Novel carbonyl reductase, gene thereof and method of using the same

Info

Publication number
AU2001278683A1
AU2001278683A1 AU2001278683A AU7868301A AU2001278683A1 AU 2001278683 A1 AU2001278683 A1 AU 2001278683A1 AU 2001278683 A AU2001278683 A AU 2001278683A AU 7868301 A AU7868301 A AU 7868301A AU 2001278683 A1 AU2001278683 A1 AU 2001278683A1
Authority
AU
Australia
Prior art keywords
benzyl
polypeptide
pyrrolidinol
amino acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001278683A
Inventor
Junzo Hasegawa
Noriyuki Kizaki
Yoshihiko Yasohara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kaneka Corp
Original Assignee
Kaneka Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kaneka Corp filed Critical Kaneka Corp
Publication of AU2001278683A1 publication Critical patent/AU2001278683A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions

Abstract

The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them. A polypeptide having the following physicochemical properties (1) to (5): (1) Action: It asymmetrically reduces N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol with NADPH as a coenzyme; (2) Optimum action pH: 4.5 to 5.5; (3) Optimum action temperature: 40 DEG C to 45 DEG C; (4) Molecular weight: About 29,000 as determined by gel filtration analysis, about 35,000 as determined by SDS-polyacrylamide gel electrophoresis analysis; (5) Inhibitor: It is inhibited by the divalent copper ion. Further, a polypeptide having the amino acid sequence shown under SEQ ID NO:1 in the sequence listing; or a polypeptide having an amino acid sequence obtainable from the amino acid sequence shown under SEQ ID NO:1 in the sequence listing by substitution, insertion, deletion and/or addition of one or more amino acids and having enzyme activity in asymmetrically reducing N-benzyl-3-pyrrolidinone to produce (S)-N-benzyl-3-pyrrolidinol.
AU2001278683A 2000-08-01 2001-08-01 Novel carbonyl reductase, gene thereof and method of using the same Abandoned AU2001278683A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2000-232756 2000-08-01
JP2000232756 2000-08-01
PCT/JP2001/006619 WO2002010399A1 (en) 2000-08-01 2001-08-01 Novel carbonyl reductase, gene thereof and method of using the same

Publications (1)

Publication Number Publication Date
AU2001278683A1 true AU2001278683A1 (en) 2002-02-13

Family

ID=18725384

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001278683A Abandoned AU2001278683A1 (en) 2000-08-01 2001-08-01 Novel carbonyl reductase, gene thereof and method of using the same

Country Status (10)

Country Link
US (1) US7033808B2 (en)
EP (1) EP1306438B1 (en)
JP (1) JP4880859B2 (en)
KR (1) KR20020056894A (en)
AT (1) ATE399208T1 (en)
AU (1) AU2001278683A1 (en)
CA (1) CA2384625A1 (en)
DE (1) DE60134557D1 (en)
ES (1) ES2309082T3 (en)
WO (1) WO2002010399A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003289229A1 (en) * 2002-12-06 2004-06-30 Kaneka Corporation Process for producing optically active 3-hydroxypropionic ester derivative
JP4884996B2 (en) * 2006-10-03 2012-02-29 国立大学法人 奈良先端科学技術大学院大学 Novel (S) -N-benzyl-3-pyrrolidinol dehydrogenase, method for producing the same, and method for producing alcohol using the same
JP5603076B2 (en) * 2007-09-26 2014-10-08 株式会社カネカ Novel glucose dehydrogenase
CN104830813A (en) 2007-12-27 2015-08-12 大塚制药株式会社 Enzymes associated with equol synthesis
US10000557B2 (en) * 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4096331A (en) * 1976-12-28 1978-06-20 A. H. Robins Company, Incorporated 1-Substituted-3-aminoethoxypyrrolidines
FI83517C (en) 1985-01-24 1991-07-25 Yamanouchi Pharma Co Ltd Process for Preparation of Right-Twisting Optical Isomer of (+ -) - 2,6-Dimethyl-4- (m-nitrophenyl) -1,4-dihydropyridin-3,5-dicarboxylic acid 3- (1-benzylpyrrolidin-3-yl) ester-5-methyl ester (YM-09730) of A-diastereomer
JPH06141876A (en) 1992-11-10 1994-05-24 Kyowa Hakko Kogyo Co Ltd Production of optically active n-benzyl-3-pyrrolidinol
JP3703928B2 (en) 1996-11-26 2005-10-05 株式会社カネカ Process for producing optically active N-benzyl-3-pyrrolidinol
JPH10150998A (en) 1996-11-26 1998-06-09 Kanegafuchi Chem Ind Co Ltd Production of n-benzyl-3-pyrrolidinol

Also Published As

Publication number Publication date
WO2002010399A1 (en) 2002-02-07
EP1306438A1 (en) 2003-05-02
US20030186412A1 (en) 2003-10-02
DE60134557D1 (en) 2008-08-07
EP1306438B1 (en) 2008-06-25
ATE399208T1 (en) 2008-07-15
KR20020056894A (en) 2002-07-10
ES2309082T3 (en) 2008-12-16
EP1306438A4 (en) 2005-03-02
JP4880859B2 (en) 2012-02-22
CA2384625A1 (en) 2002-02-07
US7033808B2 (en) 2006-04-25

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