AU2001271560A1 - Method for transformation of agaricus bisporus - Google Patents
Method for transformation of agaricus bisporusInfo
- Publication number
- AU2001271560A1 AU2001271560A1 AU2001271560A AU7156001A AU2001271560A1 AU 2001271560 A1 AU2001271560 A1 AU 2001271560A1 AU 2001271560 A AU2001271560 A AU 2001271560A AU 7156001 A AU7156001 A AU 7156001A AU 2001271560 A1 AU2001271560 A1 AU 2001271560A1
- Authority
- AU
- Australia
- Prior art keywords
- transformation
- agaricus bisporus
- protocol
- preferred
- expeditious
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000009466 transformation Effects 0.000 title abstract 4
- 241000222519 Agaricus bisporus Species 0.000 title abstract 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 abstract 2
- 241000589158 Agrobacterium Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as Agaricus bisporus. The preferred method uses an Agrobacterium-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21463000P | 2000-06-28 | 2000-06-28 | |
US60/214,630 | 2000-06-28 | ||
PCT/US2001/020566 WO2002000896A2 (en) | 2000-06-28 | 2001-06-28 | Method for transformation of agaricus bisporus |
US09/894,630 | 2001-06-28 | ||
US09/894,630 US6964866B2 (en) | 2000-06-28 | 2001-06-28 | Methods and compositions for highly efficient transformation of filamentous fungi |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001271560A1 true AU2001271560A1 (en) | 2002-01-08 |
Family
ID=26909200
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001271560A Abandoned AU2001271560A1 (en) | 2000-06-28 | 2001-06-28 | Method for transformation of agaricus bisporus |
Country Status (8)
Country | Link |
---|---|
US (2) | US6964866B2 (en) |
EP (1) | EP1409693B1 (en) |
AT (1) | ATE390488T1 (en) |
AU (1) | AU2001271560A1 (en) |
CA (1) | CA2452183C (en) |
DE (1) | DE60133422T2 (en) |
ES (1) | ES2302739T3 (en) |
WO (1) | WO2002000896A2 (en) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9597379B1 (en) | 2010-02-09 | 2017-03-21 | David Gordon Bermudes | Protease inhibitor combination with therapeutic proteins including antibodies |
US8771669B1 (en) | 2010-02-09 | 2014-07-08 | David Gordon Bermudes | Immunization and/or treatment of parasites and infectious agents by live bacteria |
US8524220B1 (en) | 2010-02-09 | 2013-09-03 | David Gordon Bermudes | Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria |
EP2558566A4 (en) * | 2010-04-14 | 2013-10-02 | Penn State Res Found | Strategies for the transgenic manipulation of filamentous fungi |
US9249418B2 (en) | 2011-10-31 | 2016-02-02 | Bp Corporation North America Inc. | Use of plant promoters in filamentous fungi |
US9737592B1 (en) | 2014-02-14 | 2017-08-22 | David Gordon Bermudes | Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment |
CN104726344B (en) * | 2014-08-25 | 2019-10-22 | 上海市农业科学院 | One kind being suitable for mushroom plant cultivation strain Shanghai F2 and its finger-print and cultural method |
CN104651389B (en) * | 2014-12-29 | 2017-06-06 | 河南农业大学 | A kind of method of liposome-mediated transfection agaricus bisporus lamella |
CN106244620A (en) * | 2016-09-09 | 2016-12-21 | 福州大学 | It is independent of medium and directly converts the method that foreign DNA enters aspergillus niger resting spore |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
CN107916273A (en) * | 2017-10-23 | 2018-04-17 | 上海市农业科学院 | A kind of method of agaricus bisporus genetic transformation |
CN109628626B (en) * | 2018-12-07 | 2022-07-26 | 东莞东阳光保健品研发有限公司 | Specific primer, kit and method for identifying morchella ladder and application of specific primer, kit and method |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2167152A1 (en) | 1993-07-13 | 1995-01-26 | Andreas Mooibroek | Production and application of transgenic mushroom mycelium and fruitbodies |
BR9807941A (en) * | 1997-04-07 | 2000-02-22 | Unilever Nv | Processes for production and for growing a processed mold, and, processed mold |
-
2001
- 2001-06-28 EP EP01950586A patent/EP1409693B1/en not_active Expired - Lifetime
- 2001-06-28 ES ES01950586T patent/ES2302739T3/en not_active Expired - Lifetime
- 2001-06-28 CA CA2452183A patent/CA2452183C/en not_active Expired - Fee Related
- 2001-06-28 AT AT01950586T patent/ATE390488T1/en active
- 2001-06-28 DE DE60133422T patent/DE60133422T2/en not_active Expired - Lifetime
- 2001-06-28 US US09/894,630 patent/US6964866B2/en not_active Expired - Lifetime
- 2001-06-28 WO PCT/US2001/020566 patent/WO2002000896A2/en active Application Filing
- 2001-06-28 AU AU2001271560A patent/AU2001271560A1/en not_active Abandoned
-
2004
- 2004-09-30 US US10/955,146 patent/US7700349B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US7700349B2 (en) | 2010-04-20 |
CA2452183A1 (en) | 2002-01-03 |
WO2002000896A2 (en) | 2002-01-03 |
DE60133422T2 (en) | 2008-08-21 |
US6964866B2 (en) | 2005-11-15 |
EP1409693A2 (en) | 2004-04-21 |
EP1409693B1 (en) | 2008-03-26 |
US20050070007A1 (en) | 2005-03-31 |
WO2002000896A3 (en) | 2003-08-28 |
ATE390488T1 (en) | 2008-04-15 |
CA2452183C (en) | 2013-12-24 |
US20020016982A1 (en) | 2002-02-07 |
ES2302739T3 (en) | 2008-08-01 |
DE60133422D1 (en) | 2008-05-08 |
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