AU2001262389A1 - Novel laccase enzyme and the gene encoding the enzyme - Google Patents

Novel laccase enzyme and the gene encoding the enzyme

Info

Publication number
AU2001262389A1
AU2001262389A1 AU2001262389A AU6238901A AU2001262389A1 AU 2001262389 A1 AU2001262389 A1 AU 2001262389A1 AU 2001262389 A AU2001262389 A AU 2001262389A AU 6238901 A AU6238901 A AU 6238901A AU 2001262389 A1 AU2001262389 A1 AU 2001262389A1
Authority
AU
Australia
Prior art keywords
enzyme
gene encoding
laccase
novel laccase
relates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001262389A
Inventor
Laura-Leena Kiiskinen
Kristiina Kruus
Marjaana Ratto
Markku Saloheimo
Liisa Viikari
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valtion Teknillinen Tutkimuskeskus
Original Assignee
Valtion Teknillinen Tutkimuskeskus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen Tutkimuskeskus filed Critical Valtion Teknillinen Tutkimuskeskus
Publication of AU2001262389A1 publication Critical patent/AU2001262389A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
    • C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
    • C12N9/0061—Laccase (1.10.3.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C5/00—Other processes for obtaining cellulose, e.g. cooking cotton linters ; Processes characterised by the choice of cellulose-containing starting materials
    • D21C5/005—Treatment of cellulose-containing material with microorganisms or enzymes
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Paper (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a new laccase enzyme, which can be isolated from the strains of the Melanocarpus genus, the M. albomyces strain in particular. The pH optimum of the enzyme is within 5-8 and the enzyme works at a temperature of 30-80° C. The isoelectric point of the enzyme is about 4.0 as determined by isoelectric focusing and the molecular weight about 80 kDa, defined by SDS-PAGE. The enzyme is especially well suited to applications, wherein the pH and temperature conditions are high. The invention also relates to a gene that encodes laccase enzyme, and a laccase enzyme produced by recombinant technology.
AU2001262389A 2000-05-23 2001-05-23 Novel laccase enzyme and the gene encoding the enzyme Abandoned AU2001262389A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FI20001240A FI113879B (en) 2000-05-23 2000-05-23 A new coating enzyme
FI20001240 2000-05-23
PCT/FI2001/000503 WO2001092498A1 (en) 2000-05-23 2001-05-23 Novel laccase enzyme and the gene encoding the enzyme

Publications (1)

Publication Number Publication Date
AU2001262389A1 true AU2001262389A1 (en) 2001-12-11

Family

ID=8558438

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001262389A Abandoned AU2001262389A1 (en) 2000-05-23 2001-05-23 Novel laccase enzyme and the gene encoding the enzyme

Country Status (16)

Country Link
US (1) US7183090B2 (en)
EP (1) EP1294859B1 (en)
JP (1) JP2003534795A (en)
CN (1) CN100510064C (en)
AT (1) ATE360684T1 (en)
AU (1) AU2001262389A1 (en)
BR (1) BR0111040A (en)
CA (1) CA2409007C (en)
DE (1) DE60128091T2 (en)
DK (1) DK1294859T3 (en)
ES (1) ES2284650T3 (en)
FI (1) FI113879B (en)
HK (1) HK1054573A1 (en)
MX (1) MXPA02011572A (en)
PT (1) PT1294859E (en)
WO (1) WO2001092498A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005021714A2 (en) 2003-08-11 2005-03-10 Diversa Corporation Laccases, nucleic acids encoding them and methods for making and using them
BRPI0516946A (en) 2004-09-21 2008-09-23 Ab Enzymes Oy laccase enzymes and their uses
WO2006032723A1 (en) * 2004-09-21 2006-03-30 Ab Enzymes Oy Novel laccase enzyme and use thereof
FI118339B (en) * 2004-09-21 2007-10-15 Ab Enzymes Oy Novel laccase capable by single treatment, in suitable conditions, of increasing lightness of desized denim at least or above as many units as sodium hypochlorite, useful for e.g. treating denim, removing stains and bleaching pulp
KR20050107352A (en) * 2005-10-25 2005-11-11 (주)에이지아이 Natural anti-cancer drug using the extracts of rhus verniciflua
PT2059587E (en) 2006-09-01 2012-01-19 Verenium Corp Laccases for pulp bio-bleaching
BRPI0721285A2 (en) * 2006-12-18 2014-03-25 Danisco Us Inc Genencor Div LACASE MEDIATORS AND METHODS OF USE
CN101568640B (en) * 2006-12-18 2012-08-15 丹尼斯科美国公司 Novel laccase, composition and use method
US8414660B2 (en) 2009-05-08 2013-04-09 University Of Florida Research Foundation, Inc. Haloferax volcanii laccase and variants and fragments thereof
CN101955952B (en) * 2010-08-02 2012-05-09 安徽大学 Bacterial laccase gene and expression and application thereof
US20140302567A1 (en) * 2011-10-14 2014-10-09 The University Of Georgia Research Foundation, Inc Methods and compositions using lignolytic enzymes and mediators to reduce and reform lignin contents in lignocellulosic biomass
CN102676550B (en) * 2012-05-17 2013-08-07 浙江商达环保有限公司 Laccase for biological treatment of papermaking wastewater and encoding gene as well as expression and application of encoding gene
US9493763B2 (en) 2012-07-09 2016-11-15 University Of Calcutta Stabilized laccase enzyme and methods of making and using the same
RU2539780C2 (en) * 2012-09-19 2015-01-27 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) RECOMBINANT TWO-DOMAIN LACCASE OF BACTERIUM Streptomyces griseoflavus Ac-993, POSSESSING HIGH THERMOSTABILITY AND ALKALINE pH OPTIMUM OF PHENOL COMPOUND OXYDATION, DNA FRAGMENT, CODING TWO-DOMAIN LACCASE OF BACTERIUM Streptomyces griseoflavus Ac-993; METHOD OF OBTAINING TWO-DOMAIN LACCASE OF BACTERIUM Streptomyces griseoflavus Ac-993
US9790529B2 (en) 2013-08-09 2017-10-17 University Of Calcutta Psychrophilic enzymes compositions and methods for making and using same
CN103501021B (en) * 2013-10-28 2015-06-10 南京工程学院 Inverter droop control method capable of achieving secondary frequency modulation and pressure regulation
CN109234174B (en) * 2018-11-12 2021-04-27 陕西科技大学 Thermophilic fungus for producing cellulase and application thereof
CN111979131B (en) * 2020-08-26 2022-04-15 中南林业科技大学 Thrombus hirsutus for efficiently decoloring lignin

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI88316C (en) 1989-07-10 1993-04-26 Enso Gutzeit Oy Process for bleaching cellulose pulp
FI903443A (en) 1990-07-06 1992-01-07 Valtion Teknillinen FRAMSTAELLNING AV LACKAS GENOM REKOMBINANTORGANISMER.
DE4137761A1 (en) 1991-05-17 1992-11-19 Call Hans Peter METHOD FOR DELIGNIFYING LIGNOCELLULOSE-CONTAINING MATERIAL, BLEACHING AND TREATING WASTEWATER BY LACCASE WITH EXTENDED EFFECTIVENESS
CA2115881C (en) * 1993-02-25 2000-05-23 Michael G. Paice Non-chlorine bleaching of kraft pulp
US5480801A (en) 1993-09-17 1996-01-02 Novo Nordisk A/S Purified PH neutral Rhizoctonia laccases and nucleic acids encoding same
AU1755495A (en) 1994-02-25 1995-09-11 Novo Nordisk A/S Laccase/peroxidase treatment of lignosulphonates
CN1192108C (en) * 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 Purified myceliophthora laccase and nucleic acid encoding same
DE69518751T2 (en) * 1994-06-03 2001-02-15 Novo Nordisk Biotech, Inc. PURIFIED SCYTALIDIUM LACASSES AND NUCLEIC ACIDS CODING THEM
US5770418A (en) * 1994-06-24 1998-06-23 Novo Nordisk A/S Purified polyporus laccases and nucleic acids encoding same
WO1996006930A1 (en) 1994-08-26 1996-03-07 Novo Nordisk A/S Coprinaceae laccases
JP2984552B2 (en) * 1994-09-02 1999-11-29 大和化成株式会社 Laccase and its production method
AU1192797A (en) * 1995-06-23 1997-01-22 Novo Nordisk A/S Oxidase, microorganisms producing the same and use of the same
JP2984584B2 (en) * 1995-08-18 1999-11-29 大和化成株式会社 Laccase II and its production method
US6008029A (en) 1995-08-25 1999-12-28 Novo Nordisk Biotech Inc. Purified coprinus laccases and nucleic acids encoding the same
DE19724039A1 (en) 1997-06-06 1998-12-10 Consortium Elektrochem Ind DNA sequences, expression of these DNA sequences, thermophilic laccases encoded by the DNA sequences and their use
DE69903025D1 (en) 1998-04-16 2002-10-24 Pulp Paper Res Inst METHOD FOR CELLULAR AND DYE OXIDATION BY OXIDASE

Also Published As

Publication number Publication date
JP2003534795A (en) 2003-11-25
WO2001092498A1 (en) 2001-12-06
FI20001240A (en) 2001-11-24
DK1294859T3 (en) 2007-08-20
DE60128091D1 (en) 2007-06-06
US20050089980A1 (en) 2005-04-28
US7183090B2 (en) 2007-02-27
BR0111040A (en) 2003-04-08
EP1294859B1 (en) 2007-04-25
CN1430668A (en) 2003-07-16
EP1294859A1 (en) 2003-03-26
ES2284650T3 (en) 2007-11-16
CA2409007A1 (en) 2001-12-06
DE60128091T2 (en) 2008-06-12
HK1054573A1 (en) 2003-12-05
CA2409007C (en) 2011-10-25
CN100510064C (en) 2009-07-08
FI113879B (en) 2004-06-30
PT1294859E (en) 2007-07-17
MXPA02011572A (en) 2004-09-06
ATE360684T1 (en) 2007-05-15

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