AU2001256186A1 - Protein preparation - Google Patents
Protein preparationInfo
- Publication number
- AU2001256186A1 AU2001256186A1 AU2001256186A AU5618601A AU2001256186A1 AU 2001256186 A1 AU2001256186 A1 AU 2001256186A1 AU 2001256186 A AU2001256186 A AU 2001256186A AU 5618601 A AU5618601 A AU 5618601A AU 2001256186 A1 AU2001256186 A1 AU 2001256186A1
- Authority
- AU
- Australia
- Prior art keywords
- protein
- transglutaminase
- solution
- preparation
- wheat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 62
- 108090000623 proteins and genes Proteins 0.000 title claims description 62
- 238000002360 preparation method Methods 0.000 title claims description 37
- 235000018102 proteins Nutrition 0.000 claims description 61
- 238000000034 method Methods 0.000 claims description 35
- 241000209140 Triticum Species 0.000 claims description 29
- 235000021307 Triticum Nutrition 0.000 claims description 29
- 108060008539 Transglutaminase Proteins 0.000 claims description 24
- 239000004615 ingredient Substances 0.000 claims description 24
- 102000003601 transglutaminase Human genes 0.000 claims description 24
- 239000000839 emulsion Substances 0.000 claims description 20
- 235000013305 food Nutrition 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 16
- 108010068370 Glutens Proteins 0.000 claims description 14
- 108010046377 Whey Proteins Proteins 0.000 claims description 12
- 235000021119 whey protein Nutrition 0.000 claims description 12
- 102000034238 globular proteins Human genes 0.000 claims description 11
- 108091005896 globular proteins Proteins 0.000 claims description 11
- 102000007544 Whey Proteins Human genes 0.000 claims description 8
- 230000002708 enhancing effect Effects 0.000 claims description 7
- 238000006911 enzymatic reaction Methods 0.000 claims description 7
- 108010064851 Plant Proteins Proteins 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 230000001804 emulsifying effect Effects 0.000 claims description 6
- 235000021118 plant-derived protein Nutrition 0.000 claims description 6
- 239000003995 emulsifying agent Substances 0.000 claims description 5
- 235000013372 meat Nutrition 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 102000004506 Blood Proteins Human genes 0.000 claims description 3
- 108010017384 Blood Proteins Proteins 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 235000013339 cereals Nutrition 0.000 claims description 3
- 235000013622 meat product Nutrition 0.000 claims description 3
- 235000013336 milk Nutrition 0.000 claims description 3
- 239000008267 milk Substances 0.000 claims description 3
- 210000004080 milk Anatomy 0.000 claims description 3
- 235000015067 sauces Nutrition 0.000 claims description 3
- 235000014347 soups Nutrition 0.000 claims description 3
- 235000015173 baked goods and baking mixes Nutrition 0.000 claims description 2
- 239000011230 binding agent Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- 239000003349 gelling agent Substances 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 230000009849 deactivation Effects 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 239000000047 product Substances 0.000 description 19
- 239000003925 fat Substances 0.000 description 11
- 235000019197 fats Nutrition 0.000 description 11
- 235000013580 sausages Nutrition 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 238000009928 pasteurization Methods 0.000 description 5
- 235000015277 pork Nutrition 0.000 description 5
- 235000019640 taste Nutrition 0.000 description 5
- 239000000499 gel Substances 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 235000019890 Amylum Nutrition 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000004945 emulsification Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 125000002252 acyl group Chemical group 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 235000019211 fat replacer Nutrition 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000012041 food component Nutrition 0.000 description 2
- 239000005417 food ingredient Substances 0.000 description 2
- 235000015244 frankfurter Nutrition 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 2
- 229920001592 potato starch Polymers 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 235000013599 spices Nutrition 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- NEAQRZUHTPSBBM-UHFFFAOYSA-N 2-hydroxy-3,3-dimethyl-7-nitro-4h-isoquinolin-1-one Chemical compound C1=C([N+]([O-])=O)C=C2C(=O)N(O)C(C)(C)CC2=C1 NEAQRZUHTPSBBM-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 235000019737 Animal fat Nutrition 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 102100030944 Protein-glutamine gamma-glutamyltransferase K Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- UHZZMRAGKVHANO-UHFFFAOYSA-M chlormequat chloride Chemical compound [Cl-].C[N+](C)(C)CCCl UHZZMRAGKVHANO-UHFFFAOYSA-M 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000015071 dressings Nutrition 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000000416 hydrocolloid Substances 0.000 description 1
- 235000015243 ice cream Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 235000010746 mayonnaise Nutrition 0.000 description 1
- 239000008268 mayonnaise Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000012434 nucleophilic reagent Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000015927 pasta Nutrition 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 229960000292 pectin Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 235000019615 sensations Nutrition 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000006276 transfer reaction Methods 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/02—Aminoacyltransferases (2.3.2)
- C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
- A23J3/08—Dairy proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/18—Vegetable proteins from wheat
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
- A23L13/42—Additives other than enzymes or microorganisms in meat products or meat meals
- A23L13/424—Addition of non-meat animal protein material, e.g. blood, egg, dairy products, fish; Proteins from microorganisms, yeasts or fungi
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/40—Meat products; Meat meal; Preparation or treatment thereof containing additives
- A23L13/42—Additives other than enzymes or microorganisms in meat products or meat meals
- A23L13/426—Addition of proteins, carbohydrates or fibrous material from vegetable origin other than sugars or sugar alcohols
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
- A23L13/60—Comminuted or emulsified meat products, e.g. sausages; Reformed meat from comminuted meat product
- A23L13/65—Sausages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Description
PROTEIN PREPARATION
The invention relates to a method for preparing a protein preparation having one or more of emulsifying, gelling, water-binding and viscosity enhancing properties. The invention further relates to the protein preparation obtainable by means of such method, to the use of such preparation and to products comprising such preparation. Fats and oils add flavors and enhance and release the flavors of other ingredients in food. Fats interact with other ingredients to develop and mold texture, mouthfeel, and the overall sensation of lubricity of food. Melting points of fat determine the food's softness, chewiness, and creaminess. Fat is a complex, highly functional ingredient that contributes to the way food tastes.
Consumers become more and more aware of the necessity to lower the amount of fat in their diet. But although the consumer is actively searching for reduced calorie, i.e. low fat, foods and beverages, he requires that they have similar taste and mouthfeel to their full calorie counterparts.
Fat replacers should thus mimic the properties of fat. Increased viscosity in a product is associated with taste as it mimics the mouthfeel of fat. Fat replacers should therefore preferably contribute to the viscosity of the food product they are part of.
In addition, many food products, like soups, sauces and meat products, such as sausages, are emulsions, in particular oil-in-water emulsions, which require emulsification, even when the amount of oil therein is lower.
Various attempts have been made to provide alternative additives that lead to a stable emulsification and increased viscosity of the product they are part of. Examples of such products are casein, hydrocolloids, carrageenan, pectin, alginate, modified
starches, various proteins and gelatin. The latter is a product derived from the bones, hides or skin of animals and may therefore be subject to the risk of BSE. None of these products lead to acceptable end products so their usefulness is limited.
In view of the above it is the object of the present invention to provide a new ingredient that has emulsifying and/or gelling and/or water-binding and/or viscosity enhancing properties. This is achieved by the invention by means of a method, comprising treating a solution of a mixture comprising a plant protein and a globular protein with the enzyme transgluta inase and optionally drying the treated mixture. The protein preparation thus obtained can be used as a food ingredient that has one or more of the desired properties. The plant protein is preferably a cereal protein, more preferably a wheat protein, and most preferably wheat gluten protein that is preferably soluble. In a preferred embodiment the ingredient has all the above properties.
It was found according to the invention that the mixture of the plant protein and globular protein should preferably be made prior to performing the enzymatic reaction. When the plant protein is wheat protein it can be any wheat protein, but is preferably a soluble wheat protein. Soluble wheat gluten protein is found to be particularly suitable. Such soluble wheat gluten protein is a product known to the person skilled in the art and can for example be obtained from Amylum (as Soluble Wheat Protein or "SWP") or ADM (Wheatpro) . The wheat gluten is preferably solubilized at neutral pH for optimal performance of the transglutaminase in the process that follows. The globular protein can be selected from various proteins, but is preferably rich in lysine. Preferred are milk whey proteins, blood proteins and egg white proteins. The advantage of whey proteins is its
outstanding nutritional protein quality, its excellent solubility and bland taste. Moreover, it is easily available as a by-product from the cheese industry.
The ratio of the wheat protein and the globular protein in the solution is between 95:5 and 5:95, preferably between 75:25 and 25:75, more preferably between 60:40 and 40:60.
The enzyme to be used in the method of the invention is transglutaminase (EC 2.3.2.13). Transglutaminase catalyzes acyl-transfer reactions in which the γ-carboxamide group of glutamine is the acyl donor and usually a nucleophilic reagent such as the e- amino group of lysine or water represents the acyl acceptor with the formation of a new isopeptide bond. Ammonia is released in the process. Transglutaminase is wide-spread in animal tissue and also occurs in microorganisms. Transglutaminase may either be calcium- dependent or calcium-independent. The latter are usually found in microorganisms, whereas the former are described to be present in various locations in the animal body, such as guinea pig liver, animal blood or fish.
Transglutaminase is currently available from Ajinomoto or Sigma.
The calcium-independent transgluta inases are preferred in view of process economics and performance.
The amount of transglutaminase lies between 0.2 and 200 units per gram protein in the solution, preferably between 1 and 100 units per gram protein in the solution, more preferably between 10 and 50 units per gram protein in the solution. The specific activity of transglutaminase is determined using the well known hydroxamate method (e.g. US-5, 156, 956) . 1 Unit of transglutaminase activity is the amount of enzyme that catalyses the formation of 1 μmol of hydroxamic acid per minute from carbobenzoxy-L-glutaminylglycine (pH 6.0, 37°C) .
The enzymatic reaction with transglutaminase is carried out for 5 minutes to 48 hours at 0 to 65 °C, preferably for 2 to 24 hours at 40 to 50 °C.
It is preferred that after the enzymatic reaction the solution is heated, firstly to pasteurize the mixture in order to achieve a hygienic condition, and secondly to inactivate the enzyme. Inactivation of the enzyme is done to avoid undesirable enzyme activity in the end product in which the ingredient is applied. Enzyme inactivation and pasteurization can be achieved by heating the solution to 80 to 140 °C for 1 second to 30 minutes.
Various methods exist for drying the protein preparation, for example spray drying, freeze drying or roller drying.
According to a further aspect thereof, the invention relates to a protein preparation having one or more of emulsifying, gelling, water-binding and viscosity enhancing properties, which is obtainable by the method of the invention. This protein preparation can be used as an emulsifier, gelling agent, water-binding agent or viscosity enhancing agent. It is self-evident that two or more of these functions can be combined in one additive or ingredient. The properties that are displayed also depend on the food product in which the ingredient is used.
The protein preparation preferably takes the form of a powder, may be a granulate, or may be part of a blend with other food ingredients, such as fats, maltodextrines, emulsifiers, carbohydrates. The ingredient of the invention is added in a suitable amount to the rest of the food product ingredients depending on the type of application used. In case the other ingredients comprise both water and an oil or fat, the ingredient of the invention will act as an emulsifier and will increase the viscosity of the mixture of ingredients. After heating of the product the ingredient will form a gel. It was found that the protein
preparation of the invention has furthermore excellent water-binding properties.
The products that may comprise the ingredient of the invention are novel by virtue of the presence therein of the ingredient. The invention thus also relates to emulsions in general, and various food products in particular, which comprise as an ingredient a protein preparation as claimed.
Food products in which the protein preparation of the invention may be used as an ingredient comprise, but are not limited to meat paste products, such as sausages, fish meat paste products, soups, sauces, such as mayonnaise and dressings, milk products, such as ice cream and yoghurt, bakery products, such as sponge cake, and pasta products.
In this application the terms "ingredient" and "protein preparation" are used interchangeably, although "protein preparation" is the more general term, whereas "ingredient" refers to the use of the preparation in a food product.
The present invention will be further elucidated in the examples that follow and that are in no way intended to limit the invention. In the examples reference is made to the figure which shows the difference between a protein preparation of the invention (left) and a mixture of its ingredients without enzymatic treatment (right) .
EXAMPLES EXAMPLE 1
Preparation of a cross-linked protein product using soluble wheat gluten protein and whey proteins
A 10% solids solution is prepared by dissolving 2 kg of soluble wheat gluten protein (SWP100, Amylum, Belgium) and 1 kg whey protein concentrate WPC 80
(Esprion 580, DMV International, The Netherlands) in 27 1. process water of 50 °C. The pH of the solution is then adjusted to 7.0 using 1M NaOH. To start the reaction 50
activity units of microbial transglutaminase (Ajinomoto, Japan) was added. The temperature during the reaction was maintained at 50 °C.
After 4 hours the reaction was stopped by pasteurisation (heated to 80 °C and held at this elevated temperature for 5 minutes) . Hereafter the solution was spray dried on a Buchi spray drier to produce sample SI.
Comparative products were made according Comparative Examples 2 and 3 to test if the benefits of the invention are specific for the mixture of these proteins.
COMPARATIVE EXAMPLE 2
Preparation of a cross-linked protein product using soluble wheat gluten protein
A 10% solids solution is prepared by dissolving
2 kg of soluble wheat gluten protein SWP100 (Amylum, Belgium) in 18 1. process water of 50 °C. The pH of the solution is then adjusted to 7.0 using 1M NaOH. The reaction was started by adding 50 activity units of microbial transglutaminase. The temperature during the reaction was maintained at 50 °C. After 4 hours the reaction was stopped by pasteurisation after which the solution was dried to produce sample S2.
COMPARATIVE EXAMPLE 3
Preparation of a cross-linked protein product using whey protein concentrate
The same process described as in comparative example 2 was used to treat WPC 80 (Esprion 580, DMV International. This produced sample S3.
EXAMPLE 4
Preparation of a cross-linked protein product using wheat gluten protein and egg white protein
A 15% solids solution is prepared by dissolving
3 kg of Wheatpro 80 (ADM, USA) and 1.5 kg dried egg whites (NIVE, The Netherlands) in 25.5 1. process water
of 50 °C. The pH of the solution is then adjusted to 7.0 using IM NaOH. To start the reaction 20 activity units of microbial transglutaminase (Ajinomoto) was added. The temperature during the reaction was maintained at 50 °C.
After 10 hours the reaction was stopped by pasteurisation. The reaction mixture was heated to 80°C and held at this elevated temperature for 5 minutes. Hereafter the solution was freeze dried. This sample showed comparable properties as sample SI.
EXAMPLE 5
Testing emulsification and gelling properties
The protein samples (20 g) to be tested were dissolved in 240 ml water using a variable speed mixer. To this solution, 300 ml soy oil is slowly added. The mixture is further emulsified for 3 minutes. Part of the emulsions were poured into small metal containers and sealed tight. The containers were statically pasteurised by heating to 85 °C for 45 minutes. After cooling to room temperature the contents were visually inspected to determine if a gel had formed. The viscosity of the emulsions was measured before pasteurisation using the Physica UDS 200 (Paar Physica) rheometer. Viscosity was measured at 25 °C using the Z3 DIN set-up. Various samples were tested to determine whether the combination of the proteins prior to the enzymatic reaction was essential. To this effect the following samples were used: the two protein preparations without addition of enzyme (SWP 100 + Esprion 580) the enzymatically treated soluble wheat gluten protein in combination with untreated whey protein (S2 + Esprion 580) - the enzymatically treated whey protein in combination with untreated soluble wheat gluten protein (SWP100 + S3) the two protein preparations after separate enzymatic treatment (S2 + S3)
- a mixture of the two proteins treated with transglutaminase (SI, according to the invention) Table 1 shows the results.
Table 1
Viscosity and gel formation
a ratio of 2:1 was used for SWPIOO and Esprion 580 in all samples
It is clear that the two proteins need to be combined prior to the enzymatic treatment with transglutaminase.
The figure further illustrates the effects seen. The emulsion made with the mixture of SWPIOO and Esprion 580 is liquid whereas the emulsion made with the
TG treated mixture (SI) forms a gel.
EXAMPLE 6
Testing emulsifying properties The emulsion forming properties were evaluated by measuring the particle size distribution of the fresh emulsions. This was done using a Malvern Mastersizer 2000. The measurement parameters were as follows: Refractive index: 1.47
Absorption: 0 Size range: 0.020 to 2000.0 μ Analysis model: general purpose (spherical)
The results are listed in the table 2,
Table 2
a ratio of 2 : 1 was used for SWPIOO and Esprion 580 in all samples D[3,2]: average particle size d[0.5]: 50% of the particles is smaller than the indicated value
The results show that emulsions made with the transglutaminase treated mixture of wheat and whey proteins (SI) have a significant smaller D[3,2], span and d(0.5) than the others. This indicates that the emulsion is mono-disperse and will have good stability.
EXAMPLE 7
Performance of the protein preparation of the invention in Frankfurter type sausage
In order to test the protein preparation of the invention in a food product, sausages were prepared. The recipe of the product is listed in table 3
Table 3 Frankfurter recipe
Ingredient % Beef brisket 18.00
Pork shoulder meat 14.00
Phosphates 0.30
Salt containing 0.6% sodium nitrite 2.00
Ice flakes 17.50 1:7:7 Pre-emulsion (*) 15.00
Pork jowl 10.10
Pork back fat 19.50
Spices 0.60
Potato starch 3.00 Total 100.00
(*) This Pre-emulsion is a pre-mix of 1 part of the wheat protein/whey protein complex (SI) , 7 parts of animal fatty tissue and 7 parts of hot water.
The procedure for preparation is as follows. A pre-emulsion preparation is prepared in a bowl chopper. The fatty tissue is shortly chopped together with the wheat protein/whey protein complex (SI) . Then, hot water of 80-90 °C is added. The mass is emulsified at maximum speed of the bowl chopper during approx. 3 minutes. The thus obtained pre-emulsion is chilled and stored in the refrigerator until it is used in the sausage batter preparation (usually within 2 days) . Sausage batter is also prepared in the bowl chopper. The beef brisket and pork shoulder meat are chopped shortly at medium speed together with the phosphates and the nitrite containing salt. Then the ice flakes are added portion wise while the bowl chopper is running at maximum speed. When the temperature reaches 4- 5°C the pre-emulsion, the pork jowl and the back fat are added. Chopping at high speed is continued and when the mass reaches a temperature of 10 °C the spices and potato
starch are added. Chopping is continued until the temperature of the mass is 12 °C. The obtained sausage batter is vacumised before it is stuffed in the selected casings. Finishing of the sausages is performed in the following manner. After stuffing of the batter in casings the sausages are subsequently:
Cured at 54 °C during 15 minutes
Dried at 40°C during 10 minutes Smoked at 54 °C during 15 minutes
Dry heated at 74 °C during 15 minutes and
Steam cooked at 80 °C during 15 minutes After chilling (and if appropriate peeling) of the sausages they are vacuum packed. The sausages thus obtained had excellent eating qualities with respect to taste and mouthfeel.
From the examples it follows that the wheat/whey protein complex of the invention is a more viscous and stable emulsion as compared to the separate ingredients. A higher viscosity of the emulsion improves handling and usability. Furthermore, less protein is required to stabilise emulsions of animal fat and water, which gives a price advantage.
Claims (28)
1. Method for preparing a protein preparation having one or more of emulsifying, gelling, water-binding and viscosity enhancing properties, comprising treating a solution of a mixture comprising a plant protein and a globular protein with the enzyme transglutaminase and optionally drying the treated mixture.
2. Method as claimed in claim 1, wherein the plant protein is a cereal protein.
3. Method as claimed in claim 2 , wherein the cereal protein is a wheat protein.
4. Method as claimed in claim 3 , wherein the wheat protein is wheat gluten protein.
5. Method as claimed in claim 4, wherein the wheat gluten protein is soluble or solubilized wheat gluten protein.
6. Method as claimed in claims 1-5, wherein the globular protein is a whey protein.
7. Method as claimed in claims 1-5, wherein the globular protein is an egg white protein.
8. Method as claimed in claims 1-5, wherein the globular protein is a blood protein or blood serum protein.
9. Method as claimed in claims 1-8, wherein the ratio of the wheat protein and the globular protein in the solution is between 95:5 and 5:95.
10. Method as claimed in claim 9, wherein the ratio of the wheat protein and the globular protein in the solution is between 75:25 and 25:75.
11. Method as claimed in claim 10, wherein the ratio of the wheat protein and the globular protein in the solution is between 60:40 and 40:60.
12. Method as claimed in claims 1-11, wherein the amount of transglutaminase is between 0.2 and 200 units per gram protein in the solution.
13. Method as claimed in claim 12 , wherein the amount of transglutaminase is between 1 and 100 units per gram protein in the solution.
14. Method as claimed in claim 13 , wherein the amount of transglutaminase is between 10 and 50 units per gram protein in the solution.
15. Method as claimed in claims 1-14, wherein the enzymatic reaction with transglutaminase is carried out for 5 minutes to 48 hours at 0 to 65 °C.
16. Method as claimed in claim 15, wherein the enzymatic reaction with transglutaminase is carried out for 2 to 24 hours at 40 to 50 °C.
17. Method as claimed in claims 1-16, wherein after the enzymatic reaction transglutaminase is deacti- vated.
18. Method as claimed in claim 17, wherein the deactivation of transglutaminase is performed by heating the solution to 80 to 140 °C for 1 second to 30 minutes.
19. Method as claimed in claims 1-18, wherein drying of the treated mixture is performed as spray drying, freeze drying or roller drying.
20. Protein preparation having one or more of emulsifying, gelling, water-binding and viscosity enhancing properties, obtainable by a method as claimed in claims 1-19.
21. Protein preparation as claimed in claim 20 for use as an emulsifier.
22. Protein preparation as claimed in claim 20 for use as an gelling agent.
23. Protein preparation as claimed in claim 20 for use as a water-binding agent.
24. Protein preparation as claimed in claim 20 for use as a viscosity enhancing agent.
25. Emulsion, comprising as an emulsifying agent a protein preparation as claimed in claims 20 and 21.
26. Food product, comprising as an ingredient a protein preparation as claimed in claims 20-24.
27. Food product as claimed in claim 26, which food product is selected from the group consisting of meat products, fish meat products, soups, sauces, milk products , bakery products .
28. Food product as claimed in claim 26, which food product is a vegetarian food product, such as a meat replacer.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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EP00200794 | 2000-03-06 | ||
EP00200794 | 2000-03-06 | ||
PCT/EP2001/002453 WO2001065948A2 (en) | 2000-03-06 | 2001-03-02 | Protein preparation |
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AU2001256186A1 true AU2001256186A1 (en) | 2001-09-17 |
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Family Applications (1)
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AU2001256186A Abandoned AU2001256186A1 (en) | 2000-03-06 | 2001-03-02 | Protein preparation |
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EP (1) | EP1263296A2 (en) |
AU (1) | AU2001256186A1 (en) |
WO (1) | WO2001065948A2 (en) |
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WO2006034856A1 (en) * | 2004-09-29 | 2006-04-06 | Nestec S.A. | Activated globular protein and its use in edible compositions |
EP1657300A1 (en) | 2004-11-10 | 2006-05-17 | N-Zyme BioTec GmbH | Beverages having reduced prolamine content and their preparation method |
CN1923050B (en) * | 2006-09-13 | 2010-09-08 | 浙江工商大学 | Method for enhancing Peruvian calamary minced fish gel characteristic and improving taste of minced fish |
ITMI20062080A1 (en) * | 2006-10-30 | 2008-04-30 | Consiglio Nazionale Ricerche | TREATMENT OF CEREAL FLOURS FOR FOOD CONSUMPTION BY CELIAC PATIENTS |
US20080181990A1 (en) * | 2007-01-26 | 2008-07-31 | Ledbetter Kati R | Compositions comprising wheat protein isolate and related methods |
US8637075B2 (en) | 2008-09-12 | 2014-01-28 | Pharma Gp Aps | Colostrum composition |
BE1022901B1 (en) * | 2015-10-20 | 2016-10-11 | Paroles Bvba | Method for manufacturing a meat product and the meat product obtained |
CN106942459B (en) * | 2017-03-08 | 2018-06-19 | 苏州欧福蛋业股份有限公司 | The preparation and application of a kind of high glue protein powder of superelevation degree of gelation for noodles |
BE1024924B1 (en) * | 2017-03-21 | 2018-08-10 | Paroles Bvba | METHOD FOR MANUFACTURING A DOMESTIC MEAT PRODUCT AND OBTAINING MEAT PRODUCT |
CN113383946B (en) * | 2021-06-24 | 2022-05-06 | 中国农业科学院农产品加工研究所 | Pickering particle dry powder and preparation method thereof |
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EP0824870A3 (en) * | 1994-06-03 | 1999-03-31 | Asama Chemical Co., Ltd. | Wheat gluten fractions |
JP3635801B2 (en) * | 1996-08-01 | 2005-04-06 | 味の素株式会社 | Milk whey protein-containing powder and processed food using the same |
AU761467B2 (en) * | 1998-06-09 | 2003-06-05 | Ajinomoto Co., Inc. | Novel enzyme-treated protein-containing food, and methods for producing the same |
-
2001
- 2001-03-02 EP EP01929403A patent/EP1263296A2/en not_active Withdrawn
- 2001-03-02 WO PCT/EP2001/002453 patent/WO2001065948A2/en not_active Application Discontinuation
- 2001-03-02 AU AU2001256186A patent/AU2001256186A1/en not_active Abandoned
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WO2001065948A2 (en) | 2001-09-13 |
WO2001065948A3 (en) | 2002-02-21 |
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