AU2001235237A1 - Starch branching enzyme - Google Patents
Starch branching enzymeInfo
- Publication number
- AU2001235237A1 AU2001235237A1 AU2001235237A AU2001235237A AU2001235237A1 AU 2001235237 A1 AU2001235237 A1 AU 2001235237A1 AU 2001235237 A AU2001235237 A AU 2001235237A AU 2001235237 A AU2001235237 A AU 2001235237A AU 2001235237 A1 AU2001235237 A1 AU 2001235237A1
- Authority
- AU
- Australia
- Prior art keywords
- nucleic acid
- plant
- acid molecule
- genetic construct
- beiib
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Claims (52)
1. An isolated nucleic acid molecule encoding wheat starch branching enzyme lib (BEIIb) .
2. An isolated nucleic acid molecule according to claim 1, in which the nucleic acid is a DNA.
3. An isolated nucleic acid molecule according to claim 1 or claim 2, in which the nucleic acid is a genomic DNA.
4. An isolated nucleic acid molecule according to claim 3, in which the nucleic acid is present in any one of clones Gl , G2 , G7 to G9 , or Hi to H10.
5. An isolated nucleic acid molecule according to claim 1 or claim 2, in which the nucleic acid is a cDNA.
6. An isolated nucleic acid molecule according to claim 5, which has (a) the sequence depicted in any one of Figure 8 (SEQ ID NO 5) , Figure 9 (SEQ ID NO 6) , or SEQ ID NO 10;
(b) a nucleic acid molecule capable of hybridising to at least one of the sequences in (a) under at least low stringency hybridization conditions; or
(c) a nucleic acid molecule with at least 70% sequence identity to at least one of the sequences in (a) .
7. An isolated nucleic acid molecule according to claim 6, which has
(a) the sequence depicted in SEQ ID NO 10; (b) a nucleic acid molecule capable of hybridising to SEQ ID NO: 10 under at least low stringency hybridization conditions; or
(c) a nucleic acid molecule with at least 70% sequence identity to SEQ ID NO: 10. - 45 -
8. An isolated nucleic acid molecule according to claim 6 or claim 7, in which the nucleic acid molecule is capable of hybridizing to at least one of the sequences in (a) under high stringency conditions, or has at least 80% sequence identity thereto.
9. An isolated nucleic acid molecule according to any one of claims 6 to 8 , in which the nucleic acid molecule has at least 90% sequence identity to at least one of the sequences in (a) .
10. A promoter sequence of a genomic DNA according to any one of claims 1 to 3.
11. A genetic construct comprising a nucleic acid sequence according to any one of claims 1 to 9 , a biologically- active fragment thereof, or a fragment thereof encoding a biologically-active fragment of BEIIb operably linked to one or more nucleic acid sequences which are capable of facilitating expression of BEIIb in a plant.
12. A genetic construct according to claim 11, in which the plant is a cereal plant.
13. A genetic construct according to claim 11 or claim 12, in which the construct is a plasmid or a vector.
14. A genetic construct according to any one of claims 11 to 13, in which the construct is one suitable for use in transformation of a plant.
15. A genetic construct according to claim 13 or claim 14, in which the vector is a bacterium of the genus Agroba cterium.
16. A genetic construct according to claim 15, in which the bacterium is Agrobacterium tumefaciens .
17. A genetic construct for targeting of a desired gene to endosperm of a cereal plant, and/or for modulating the time of expression of a desired gene in endosperm of a cereal - 46 -
plant, comprising a BEIIb promoter, operatively linked to a nucleic acid sequence encoding a desired protein, and optionally also operatively linked to one or more additional targeting sequences and/or one or more 3 ' untranslated sequences.
18. A genetic construct according to claim 17, in which the desired protein is encoded by a gene which is capable of being expressed in the endosperm of a cereal plant.
19. A genetic construct according to any one of claims 16 to 18, in which the desired protein is an enzyme of the starch biosynthetic pathway.
20. A genetic construct according to any one of claims 16 to 19, in which the nucleic acid encoding the desired protein is in the sense orientation.
21. A genetic construct according to claim 20, in which the sense sequence is selected from the group consisting of bacterial isoamylase, bacterial glycogen synthase, and wheat high molecular weight glutenin Bxl7.
22. A genetic construct according to any one of claims 16 to 19, in which the nucleic acid encoding the desired protein is in the anti-sense orientation.
23. A genetic construct according to claim 22, in which the antisense sequence is selected from the group consisting of GBSS, starch debranching enzyme, SBE II, low molecular weight glutenin, and grain softness protein I.
24. A wheat BEIIb polypeptide, comprising an amino acid sequence encoded by a nucleic acid molecule according to any one of claims 1 to 9 , or a polypeptide having at least 70% amino acid sequence identity thereto, and having the biological activity of BEIIb. - 47 -
25. A wheat BEIIb polypeptide according to claim 24, having at least 80% amino acid sequence identity to an amino acid sequence encoded by a nucleic acid molecule according to any one of claims 1 to 9.
26. A wheat BEIIb polypeptide according to claim 26, having at least 90% amino acid sequence identity to an amino acid sequence encoded by a nucleic acid molecule according to any one of claims 1 to 9.
27. An antibody directed against a BEII polypeptide according to any one of claims 24 to 26.
28. An antibody according to claim 27, which is polyclonal .
29. An antibody according to claim 27, which is monoclonal .
30. An antibody according to any one of claims 27 to 29, which is raised against a sequence as set out in SEQ ID NO 7, SEQ ID NO 8, or SEQ ID NO 9.
31. A plant cell transformed by a genetic construct according to any one of claims 11 to 23.
32. A plant cell according to claim 31, which also comprises a null allele for a gene selected from the group consisting of GBSS, BEIIa, and SSII.
33. A plant derived from a cell according to claim 31 or claim 32.
34. A plant comprising one or more BEIIb null alleles, in combination with one or more other null alleles selected from the group consisting of BEIIa, GBSS, SSII and BEI, and optionally also comprising a BEIIa or BEIIb gene expressed in either the sense or the anti-sense orientation.
35. A plant according to claim 33 or claim 34, which is a cereal plant . - 48 -
36. A plant according to claim 35, which is wheat or barley.
37. A product produced from a plant according to any one of claims 33 to 36.
38. A product according to claim 37, selected from the group consisting of whole grain, part grain, flour and starch.
39. A product according to claim 37 or claim 38, which is a food.
40. A food product according to claim 39, selected from the group consisting of unleavened breads, pasta, noodles, breakfast cereals, snack foods, cakes, pastries, and foods containing flour- or starch-based sauces .
41. A product according to claim 37 or claim 38, which is not a food.
42. A non-food product according to claim 41, selected from the group consisting of films, coating, adhesives, building materials, disposable goods, and packaging materials .
43. A method of modifying the characteristics of starch produced by a plant, comprising the steps of: a) increasing the level of expression of BEIIb in the plant, or b) decreasing the level of expression of BEIIb in the plant.
44. A method according to claim 43, in which the plant is a cereal plant.
45. A method according to claim 44, in which the plant is wheat or barley. - 49 -
46. A method according to any one of claims 43 to 45,' in which the branching of the amylopectin component of starch is modified.
47. A method according to any one of claims 43 to 46, in which a plant with high amylose content is produced.
48. A method according to any one of claims 43 to 46, in which a plant with high amylose content is produced.
49. A method according to any one of claims 43 to 46, in which a plant with low amylopectin content is produced.
50. A method of targeting expression of a desired gene to the endosperm of a cereal plant, comprising the step of transforming the plant with a genetic construct according to any one of claims 11 to 23.
51. A method of identifying a null or altered allele encoding an enzyme of the starch biosynthetic pathway, comprising the step of subjecting DNA from a plant suspected to possess such an allele to a DNA fingerprinting or amplification assay which utilises at least one DNA probe comprising a nucleic acid molecule according to any one of claims 1 to 10.
52. An oligonucleotide probe selected from the group consisting of SEQ ID NOS: 11 to 17.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2001235237A AU2001235237B2 (en) | 2000-02-21 | 2001-02-21 | Starch branching enzyme |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPQ5742A AUPQ574200A0 (en) | 2000-02-21 | 2000-02-21 | Starch branching enzyme |
| AUPQ5742 | 2000-02-21 | ||
| PCT/AU2001/000175 WO2001062934A1 (en) | 2000-02-21 | 2001-02-21 | Starch branching enzyme |
| AU2001235237A AU2001235237B2 (en) | 2000-02-21 | 2001-02-21 | Starch branching enzyme |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2001235237A1 true AU2001235237A1 (en) | 2001-11-15 |
| AU2001235237B2 AU2001235237B2 (en) | 2007-02-08 |
Family
ID=3819853
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AUPQ5742A Abandoned AUPQ574200A0 (en) | 2000-02-21 | 2000-02-21 | Starch branching enzyme |
| AU2001235237A Expired AU2001235237B2 (en) | 2000-02-21 | 2001-02-21 | Starch branching enzyme |
| AU3523701A Pending AU3523701A (en) | 2000-02-21 | 2001-02-21 | Starch branching enzyme |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AUPQ5742A Abandoned AUPQ574200A0 (en) | 2000-02-21 | 2000-02-21 | Starch branching enzyme |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU3523701A Pending AU3523701A (en) | 2000-02-21 | 2001-02-21 | Starch branching enzyme |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US7667114B2 (en) |
| EP (1) | EP1263961B1 (en) |
| JP (1) | JP2003523773A (en) |
| AU (3) | AUPQ574200A0 (en) |
| CA (1) | CA2400710C (en) |
| ES (1) | ES2433471T3 (en) |
| NZ (1) | NZ520904A (en) |
| WO (1) | WO2001062934A1 (en) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPQ005299A0 (en) | 1999-04-29 | 1999-05-27 | Commonwealth Scientific And Industrial Research Organisation | Novel genes encoding wheat starch synthases and uses therefor |
| AU1480402A (en) | 2000-11-09 | 2002-05-21 | Commw Scient Ind Res Org | Barley with reduced SSII activity and starch containing products with a reduced amylopectin content |
| JP4308002B2 (en) * | 2001-09-10 | 2009-08-05 | 独立行政法人科学技術振興機構 | Starch synthases |
| AUPS219802A0 (en) * | 2002-05-09 | 2002-06-06 | Commonwealth Scientific And Industrial Research Organisation | Barley with altered branching enzyme activity and starch and starch containing products with a reduced amylopectin content |
| WO2005001098A1 (en) * | 2003-06-30 | 2005-01-06 | Commonwealth Scientific And Industrial Research Organisation | Wheat with altered branching enzyme activity and starch and starch containing products derived thereform |
| EP1692289B1 (en) * | 2003-10-27 | 2012-01-25 | Commonwealth Scientific And Industrial Research Organisation | Rice and products thereof having starch with an increased proportion of amylose |
| US7700139B2 (en) | 2004-12-30 | 2010-04-20 | Commonwealth Scientific And Industrial Research Organization | Method and means for improving bowel health |
| JP5638736B2 (en) * | 2004-12-30 | 2014-12-10 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション | Methods and means for improving intestinal health |
| US7993686B2 (en) * | 2004-12-30 | 2011-08-09 | Commonwealth Scientific And Industrial Organisation | Method and means for improving bowel health |
| JP2009543561A (en) | 2006-07-14 | 2009-12-10 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション | Modification of fatty acid composition of rice |
| MX2010005937A (en) | 2007-11-27 | 2010-06-15 | Commw Scient Ind Res Org | Plants with modified starch metabolism. |
| CA2653883C (en) | 2008-07-17 | 2022-04-05 | Colin Leslie Dow Jenkins | High fructan cereal plants |
| ES2603530T3 (en) | 2008-07-21 | 2017-02-28 | Commonwealth Scientific And Industrial Research Organisation | Improved cottonseed oil and uses |
| CA2768737A1 (en) * | 2008-07-21 | 2010-01-28 | Commonwealth Scientific And Industrial Research Organisation | Improved vegetable oils and uses therefor |
| KR101806299B1 (en) | 2009-07-30 | 2017-12-07 | 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 | Barley and uses thereof |
| RU2619636C2 (en) | 2010-11-04 | 2017-05-17 | Ариста Сириэл Текнолоджиз Пти Лтд | High amylose wheat |
| AU2012212404B2 (en) | 2011-02-03 | 2016-05-12 | The Healthy Grain Pty Limited | Barley with modified SSIII |
| WO2013052499A1 (en) | 2011-10-04 | 2013-04-11 | Arcadia Biosciences, Inc. | Wheat with increased resistant starch levels |
| JP6346093B2 (en) | 2011-11-04 | 2018-06-20 | アリスタ シリアル テクノロジーズ プロプライエタリー リミテッドＡｒｉｓｔａ Ｃｅｒｅａｌ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｐｔｙ Ｌｔｄ | High amylose wheat |
| SG11201406843WA (en) | 2012-04-25 | 2014-11-27 | Commw Scient Ind Res Org | High oleic acid oils |
| CA3216668A1 (en) | 2014-07-07 | 2016-01-14 | Nuseed Global Innovation Ltd | Processes for producing industrial products from plant lipids |
| CA2928448C (en) * | 2015-08-06 | 2023-08-01 | 0959690 B.C. Ltd. Doing Business As Mat Processing Systems | Mat cleaning and drying apparatus and method |
| AU2017320470B2 (en) | 2016-09-02 | 2023-11-23 | Nuseed Global Innovation Ltd | Plants with modified traits |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RO118718B1 (en) * | 1995-12-20 | 2003-09-30 | E.I. Du Pont De Nemours And Company | Process for obtaining a genetically modified corn plant with capacity of synthetizing starch with modified structure |
| US5866793A (en) * | 1996-06-03 | 1999-02-02 | National Research Council Of Canada | Promoter for expressing foreign genes in monocotyledonous plants |
| SE509968C2 (en) * | 1997-02-14 | 1999-03-29 | Ericsson Telefon Ab L M | Variable amplifier optical amplifier |
| EP1012250B1 (en) * | 1997-09-12 | 2008-12-17 | Commonwealth Scientific And Industrial Research Organisation | Regulation of gene expression in plants |
| DE69942032D1 (en) | 1998-09-10 | 2010-04-01 | Monsanto Uk Ltd | STARCH BRANCH II (ISO) SBE-IIA AND SBE-IIB ISOFORMS FROM WHEAT |
-
2000
- 2000-02-21 AU AUPQ5742A patent/AUPQ574200A0/en not_active Abandoned
-
2001
- 2001-02-21 EP EP01907236.2A patent/EP1263961B1/en not_active Expired - Lifetime
- 2001-02-21 AU AU2001235237A patent/AU2001235237B2/en not_active Expired
- 2001-02-21 NZ NZ520904A patent/NZ520904A/en not_active IP Right Cessation
- 2001-02-21 AU AU3523701A patent/AU3523701A/en active Pending
- 2001-02-21 CA CA2400710A patent/CA2400710C/en not_active Expired - Lifetime
- 2001-02-21 US US10/204,347 patent/US7667114B2/en not_active Expired - Lifetime
- 2001-02-21 ES ES01907236T patent/ES2433471T3/en not_active Expired - Lifetime
- 2001-02-21 WO PCT/AU2001/000175 patent/WO2001062934A1/en active IP Right Grant
- 2001-02-21 JP JP2001562708A patent/JP2003523773A/en active Pending
-
2010
- 2010-02-17 US US12/707,437 patent/US20110010807A1/en not_active Abandoned
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