AU1953492A - Immobilization of nucleic acids - Google Patents

Immobilization of nucleic acids

Info

Publication number
AU1953492A
AU1953492A AU19534/92A AU1953492A AU1953492A AU 1953492 A AU1953492 A AU 1953492A AU 19534/92 A AU19534/92 A AU 19534/92A AU 1953492 A AU1953492 A AU 1953492A AU 1953492 A AU1953492 A AU 1953492A
Authority
AU
Australia
Prior art keywords
nucleic acids
immobilization
immobilisable
nucleotides
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
AU19534/92A
Other versions
AU640089B2 (en
Inventor
Sibylle Berner
Stefanie Koehler
Cornelia Kruse-Mueller
Rudolf Seibl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of AU1953492A publication Critical patent/AU1953492A/en
Application granted granted Critical
Publication of AU640089B2 publication Critical patent/AU640089B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Abstract

A method for detecting nucleic acids in which a nucleic acid probe with two or more nucleotides which are modified by immobilisable groups and which are not directly adjacent in the nucleotide sequence is used. <IMAGE>
AU19534/92A 1991-07-16 1992-07-09 Immobilization of nucleic acids Ceased AU640089B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4123540A DE4123540A1 (en) 1991-07-16 1991-07-16 IMMOBILIZATION OF NUCLEIC ACIDS
DE4123540 1991-07-16

Publications (2)

Publication Number Publication Date
AU1953492A true AU1953492A (en) 1993-04-29
AU640089B2 AU640089B2 (en) 1993-08-12

Family

ID=6436267

Family Applications (1)

Application Number Title Priority Date Filing Date
AU19534/92A Ceased AU640089B2 (en) 1991-07-16 1992-07-09 Immobilization of nucleic acids

Country Status (15)

Country Link
EP (1) EP0523557B1 (en)
JP (1) JP2644419B2 (en)
KR (1) KR960004039B1 (en)
AT (1) ATE132538T1 (en)
AU (1) AU640089B2 (en)
CA (1) CA2073989A1 (en)
DE (2) DE4123540A1 (en)
DK (1) DK0523557T3 (en)
ES (1) ES2083629T3 (en)
FI (1) FI923239A (en)
IE (1) IE922298A1 (en)
IL (1) IL102487A0 (en)
NO (1) NO922794L (en)
NZ (1) NZ243525A (en)
ZA (1) ZA925259B (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4239531A1 (en) * 1992-11-25 1994-05-26 Gabor Dr Igloi Reagent for coupling various substances to nucleic acids and process for its production
CA2119701A1 (en) * 1993-04-16 1994-10-17 Colleen M. Nycz Nucleic acid assay procedure
US6821727B1 (en) 1993-11-15 2004-11-23 Applera Corporation Hybridization assay using self-quenching fluorescence probe
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
DE19516196A1 (en) * 1995-05-08 1996-11-14 Boehringer Mannheim Gmbh Method for the quantitative detection of nucleic acids
DE19525632C2 (en) * 1995-07-14 1997-07-17 Bag Biolog Analysensystem Gmbh Sequence-specific nucleic acid detection method and reagent system for its implementation
DE19533354C2 (en) * 1995-09-08 1999-07-01 Derya Dr Ozkan Methods and their applications for sequence-specific detection and quantification of nucleic acids
DE19537952A1 (en) 1995-10-12 1997-04-17 Boehringer Mannheim Gmbh Method for the detection of an analyte
DE19548680A1 (en) * 1995-12-23 1997-06-26 Boehringer Mannheim Gmbh Method for the quantitative detection of specific nucleic acid sequences
US5840879A (en) * 1996-12-06 1998-11-24 Wang; Edge R. Reagents and solid supports for improved synthesis and labeling of polynucleotides
ES2129001B1 (en) * 1997-07-18 2000-04-16 Pharmagen S A METHOD FOR THE DETECTION AND VISUALIZATION OF A SPECIFIC DNA SEQUENCE.
DE19835856A1 (en) * 1998-08-07 2000-02-17 W Kurt Roth Oligonucleotide primers and probes, for the detection of hepatitis B virus, are used to amplify, by polymerase chain reaction, a section of the hepatitis B virus genome
DE102022204638A1 (en) * 2022-05-12 2023-11-16 Robert Bosch Gesellschaft mit beschränkter Haftung Method and microfluidic device for denaturing a nucleic acid

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4582789A (en) * 1984-03-21 1986-04-15 Cetus Corporation Process for labeling nucleic acids using psoralen derivatives
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
JPS638396A (en) * 1986-06-30 1988-01-14 Wakunaga Pharmaceut Co Ltd Poly-labeled oligonucleotide derivative
JPS6455198A (en) * 1987-06-11 1989-03-02 Nippon D P C Corp Method for hybridization of nucleic acid
US5082830A (en) * 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences

Also Published As

Publication number Publication date
DK0523557T3 (en) 1996-05-06
ZA925259B (en) 1994-01-17
JP2644419B2 (en) 1997-08-25
KR930002371A (en) 1993-02-23
ES2083629T3 (en) 1996-04-16
EP0523557B1 (en) 1996-01-03
EP0523557A1 (en) 1993-01-20
ATE132538T1 (en) 1996-01-15
DE4123540A1 (en) 1993-01-21
NZ243525A (en) 1993-11-25
DE59204886D1 (en) 1996-02-15
IE922298A1 (en) 1993-01-27
NO922794L (en) 1993-01-18
FI923239A0 (en) 1992-07-15
NO922794D0 (en) 1992-07-15
JPH05199899A (en) 1993-08-10
KR960004039B1 (en) 1996-03-25
CA2073989A1 (en) 1993-01-17
AU640089B2 (en) 1993-08-12
FI923239A (en) 1993-01-17
IL102487A0 (en) 1993-01-14

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