ATE469981T1 - Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten - Google Patents

Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten

Info

Publication number
ATE469981T1
ATE469981T1 AT97934976T AT97934976T ATE469981T1 AT E469981 T1 ATE469981 T1 AT E469981T1 AT 97934976 T AT97934976 T AT 97934976T AT 97934976 T AT97934976 T AT 97934976T AT E469981 T1 ATE469981 T1 AT E469981T1
Authority
AT
Austria
Prior art keywords
protein
tests
substrates
phosphorylated
fluorescent
Prior art date
Application number
AT97934976T
Other languages
English (en)
Inventor
Roger Tsien
Andrew Cubitt
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/679,865 external-priority patent/US5912137A/en
Priority claimed from US08/680,876 external-priority patent/US5925558A/en
Application filed by Univ California filed Critical Univ California
Application granted granted Critical
Publication of ATE469981T1 publication Critical patent/ATE469981T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT97934976T 1996-07-16 1997-07-16 Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten ATE469981T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US68087796A 1996-07-16 1996-07-16
US08/679,865 US5912137A (en) 1996-07-16 1996-07-16 Assays for protein kinases using fluorescent
US08/680,876 US5925558A (en) 1996-07-16 1996-07-16 Assays for protein kinases using fluorescent protein substrates
PCT/US1997/012410 WO1998002571A1 (en) 1996-07-16 1997-07-16 Assays for protein kinases using fluorescent protein substrates

Publications (1)

Publication Number Publication Date
ATE469981T1 true ATE469981T1 (de) 2010-06-15

Family

ID=27418360

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97934976T ATE469981T1 (de) 1996-07-16 1997-07-16 Tests auf proteinkinasen mit fluoreszierenden proteinsubstraten

Country Status (5)

Country Link
EP (1) EP0915989B1 (de)
AT (1) ATE469981T1 (de)
AU (1) AU3801997A (de)
DE (1) DE69739898D1 (de)
WO (1) WO1998002571A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6900304B2 (en) * 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US8669074B2 (en) 1996-01-31 2014-03-11 The Regents Of The University Of California Chimeric phosphorylation indicator
DE69840146D1 (de) * 1997-03-14 2008-12-04 Univ California Fluoreszenzprotein-sensoren zur erkennung von analyten
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
WO1998045704A2 (en) 1997-04-07 1998-10-15 Bioimage A/S A method for extracting quantitative information relating to an influence on a cellular response
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
FR2768817B1 (fr) * 1997-09-19 1999-12-10 Cis Bio Int Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
US6150176A (en) * 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
EP1115734A4 (de) * 1998-09-24 2004-07-21 Univ Duke Methoden zur messung von protein-protein wechselwirkungen in lebenden zellen
EP1121593A2 (de) * 1998-10-15 2001-08-08 BioImage A/S Verfahren zur ableitung quantitativer informationen über einen einfluss auf eine zelluläre respons
AU2813500A (en) * 1999-02-25 2000-09-14 Cyclacel Limited Compositions and methods for monitoring the modification of natural binding partners
GB9904407D0 (en) * 1999-02-25 1999-04-21 Fluorescience Ltd Compositions and methods for monitoring the modification of engineered binding partners
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
NL1012782C2 (nl) * 1999-08-05 2001-02-06 Keygene Nv Verbeterde gfp-variant, bruikbaar als markering, en gfp-construct.
US7338774B2 (en) 2001-02-14 2008-03-04 Sysmex Corporation Method for determining activity of cell cycle regulatory factor and method for diagnosing cancer using the same
WO2002077011A2 (en) * 2001-03-12 2002-10-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation-reduction sensitive green fluorescent protein variants
JP3829252B2 (ja) 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
WO2003012068A2 (en) 2001-08-01 2003-02-13 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
FR2828692B1 (fr) * 2001-08-16 2004-07-09 France Etat Armement Procede d'ingenierie genetique pour le suivi en temps reel de l'activation de proteines specifiques de transduction de signaux cellulaires
GB0208987D0 (en) * 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring protein kinase and phosphatase activity
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
GB0421285D0 (en) 2004-09-24 2004-10-27 Univ Nottingham Improvements in high content screening
US8680235B2 (en) 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8916806D0 (en) * 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
US5723286A (en) * 1990-06-20 1998-03-03 Affymax Technologies N.V. Peptide library and screening systems
US5532167A (en) * 1994-01-07 1996-07-02 Beth Israel Hospital Substrate specificity of protein kinases
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances

Also Published As

Publication number Publication date
WO1998002571A1 (en) 1998-01-22
AU3801997A (en) 1998-02-09
EP0915989A4 (de) 2005-10-12
EP0915989B1 (de) 2010-06-02
EP0915989A1 (de) 1999-05-19
DE69739898D1 (de) 2010-07-15

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