ATE462007T1 - Produktion von minicircle-vektoren - Google Patents

Produktion von minicircle-vektoren

Info

Publication number
ATE462007T1
ATE462007T1 AT04730974T AT04730974T ATE462007T1 AT E462007 T1 ATE462007 T1 AT E462007T1 AT 04730974 T AT04730974 T AT 04730974T AT 04730974 T AT04730974 T AT 04730974T AT E462007 T1 ATE462007 T1 AT E462007T1
Authority
AT
Austria
Prior art keywords
production
minicircle vectors
minicircle
vectors
Prior art date
Application number
AT04730974T
Other languages
English (en)
Inventor
Peter Mayrhofer
Wolfgang Jechlinger
Werner Lubitz
Original Assignee
Peter Mayrhofer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32686612&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE462007(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Peter Mayrhofer filed Critical Peter Mayrhofer
Application granted granted Critical
Publication of ATE462007T1 publication Critical patent/ATE462007T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
AT04730974T 2003-05-08 2004-05-04 Produktion von minicircle-vektoren ATE462007T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0070003A AT412400B (de) 2003-05-08 2003-05-08 Minicircle-herstellung
PCT/EP2004/004721 WO2004099420A1 (en) 2003-05-08 2004-05-04 Minicircle vector production

Publications (1)

Publication Number Publication Date
ATE462007T1 true ATE462007T1 (de) 2010-04-15

Family

ID=32686612

Family Applications (2)

Application Number Title Priority Date Filing Date
AT0070003A AT412400B (de) 2003-05-08 2003-05-08 Minicircle-herstellung
AT04730974T ATE462007T1 (de) 2003-05-08 2004-05-04 Produktion von minicircle-vektoren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT0070003A AT412400B (de) 2003-05-08 2003-05-08 Minicircle-herstellung

Country Status (7)

Country Link
US (2) US8647863B2 (de)
EP (2) EP1620559B2 (de)
AT (2) AT412400B (de)
AU (1) AU2004236352B2 (de)
CA (1) CA2524057A1 (de)
DE (1) DE602004026174D1 (de)
WO (1) WO2004099420A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT412400B (de) * 2003-05-08 2005-02-25 Mayrhofer Peter Mag Dr Minicircle-herstellung
CA2641026A1 (en) * 2006-02-01 2007-08-09 The Johns Hopkins University Polypeptide-nucleic acid conjugate for immunoprophylaxis or immunotherapy for neoplastic or infectious disorders
CN102317303A (zh) * 2007-07-31 2012-01-11 约翰·霍普金斯大学 用于肿瘤或感染性疾病的免疫预防或免疫治疗的多肽-核酸偶联物
EP2439276A1 (de) 2010-10-05 2012-04-11 Rentschler Biotechnologie GmbH Verfahren zur semi-synthetischen Herstellung hochreiner "MiniCircle" DNA-Vektoren aus Plasmiden
DE102011118018B4 (de) 2011-10-25 2017-10-26 Plasmidfactory Gmbh & Co. Kg Minicircles mit Transpositionskassetten und ihre Verwendung zur Transformation von Zellen
EP2735614A1 (de) * 2012-11-22 2014-05-28 Centre National De La Recherche Scientifique In-vitro-Herstellung von DNA-Miniringen mit weniger als 250 Basispaaren
GB201307075D0 (en) 2013-04-19 2013-05-29 Mayrhofer Peter Plasmid for minicircle production
DE102013220859B4 (de) 2013-10-15 2016-09-08 Plasmidfactory Gmbh & Co. Kg Minicircles mit viralen Expressionskassetten und ihre Verwendung zur Transformation von Zellen zur Erzeugung rekombinanter Viren oder viraler Genvektoren
PT107663B (pt) * 2014-05-28 2019-02-26 Inst Superior Tecnico Processo para a purificação de minicírculos
CN109536521A (zh) * 2018-11-26 2019-03-29 吉林农业大学 一种微环dna及其制备方法与应用
JP2022519308A (ja) 2019-02-06 2022-03-22 フレッド ハッチンソン キャンサー リサーチ センター その中の核酸分子を差次的にメチル化するように操作されたミニサークル産生細菌
WO2021055760A1 (en) 2019-09-18 2021-03-25 Intergalactic Therapeutics, Inc. Synthetic dna vectors and methods of use
WO2023122625A2 (en) * 2021-12-20 2023-06-29 Intergalactic Therapeutics, Inc. Production of gene therapy vector in engineered bacteria
US20250354159A1 (en) * 2022-06-13 2025-11-20 Nanjing GenScript Biotech Co., Ltd. Wild-type-mutant pi protein switching expression system capable of increasing efficiency of preparing screening-tag-free plasmid

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622866A (en) * 1994-06-23 1997-04-22 Merck & Co., Inc. Expression cassettes useful in construction of integrative and replicative expression vectors for Streptomyces
FR2731014B1 (fr) 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
WO2002020823A2 (en) * 2000-09-07 2002-03-14 The Salk Institute For Biological Studies Methods and compositions for visualizing and interfering with chromosomal tethering of extrachromosomal molecules
GB0108968D0 (en) 2001-04-10 2001-05-30 Imp College Innovations Ltd Methods
WO2002083889A2 (en) * 2001-04-10 2002-10-24 Imperial College Innovations Limited Methods for the production of minicircles
AT412400B (de) * 2003-05-08 2005-02-25 Mayrhofer Peter Mag Dr Minicircle-herstellung

Also Published As

Publication number Publication date
WO2004099420A8 (en) 2006-01-05
EP2213744A1 (de) 2010-08-04
ATA7002003A (de) 2004-07-15
EP2213744B1 (de) 2015-07-01
EP1620559B1 (de) 2010-03-24
AU2004236352A1 (en) 2004-11-18
DE602004026174D1 (de) 2010-05-06
AU2004236352B2 (en) 2009-01-08
US8911974B2 (en) 2014-12-16
US20140065705A1 (en) 2014-03-06
US8647863B2 (en) 2014-02-11
US20070031378A1 (en) 2007-02-08
EP1620559A1 (de) 2006-02-01
WO2004099420A1 (en) 2004-11-18
CA2524057A1 (en) 2004-11-18
AT412400B (de) 2005-02-25
EP1620559B2 (de) 2021-04-14
EP2213744B2 (de) 2019-01-02

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