ATE452974T1 - Verfahren zur herstellung einer vollängen-cdna durch ligation eines adapters mit einem kohäsiven ende - Google Patents
Verfahren zur herstellung einer vollängen-cdna durch ligation eines adapters mit einem kohäsiven endeInfo
- Publication number
- ATE452974T1 ATE452974T1 AT04805284T AT04805284T ATE452974T1 AT E452974 T1 ATE452974 T1 AT E452974T1 AT 04805284 T AT04805284 T AT 04805284T AT 04805284 T AT04805284 T AT 04805284T AT E452974 T1 ATE452974 T1 AT E452974T1
- Authority
- AT
- Austria
- Prior art keywords
- length cdna
- full
- producing
- rna
- adapter
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 2
- 102000003960 Ligases Human genes 0.000 abstract 1
- 108090000364 Ligases Proteins 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000030609 dephosphorylation Effects 0.000 abstract 1
- 238000006209 dephosphorylation reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 238000010839 reverse transcription Methods 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Materials For Photolithography (AREA)
- Photoreceptors In Electrophotography (AREA)
- Diaphragms For Electromechanical Transducers (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0312458A FR2861405A1 (fr) | 2003-10-24 | 2003-10-24 | METHODE DE PRODUCTION D'ADNc PLEINE LONGUEUR PAR LIGATURATION D'ADAPTATEUR A EXTREMITE COHESIVE |
| PCT/FR2004/002722 WO2005045031A1 (fr) | 2003-10-24 | 2004-10-22 | METHODE DE PRODUCTION D’ADNc PLEINE LONGUEUR PAR LIGATURATION D’ADAPTATEUR A EXTREMITE COHESIVE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE452974T1 true ATE452974T1 (de) | 2010-01-15 |
Family
ID=34400763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04805284T ATE452974T1 (de) | 2003-10-24 | 2004-10-22 | Verfahren zur herstellung einer vollängen-cdna durch ligation eines adapters mit einem kohäsiven ende |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1678300B1 (de) |
| AT (1) | ATE452974T1 (de) |
| DE (1) | DE602004024813D1 (de) |
| ES (1) | ES2338564T3 (de) |
| FR (1) | FR2861405A1 (de) |
| WO (1) | WO2005045031A1 (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160083724A1 (en) * | 2014-09-24 | 2016-03-24 | University Of Southern California | Methods for sample preparation |
| GB201808554D0 (en) * | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3337748B2 (ja) * | 1992-09-25 | 2002-10-21 | 財団法人神奈川科学技術アカデミー | 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法 |
-
2003
- 2003-10-24 FR FR0312458A patent/FR2861405A1/fr not_active Withdrawn
-
2004
- 2004-10-22 WO PCT/FR2004/002722 patent/WO2005045031A1/fr not_active Ceased
- 2004-10-22 EP EP04805284A patent/EP1678300B1/de not_active Expired - Lifetime
- 2004-10-22 ES ES04805284T patent/ES2338564T3/es not_active Expired - Lifetime
- 2004-10-22 DE DE602004024813T patent/DE602004024813D1/de not_active Expired - Lifetime
- 2004-10-22 AT AT04805284T patent/ATE452974T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| ES2338564T3 (es) | 2010-05-10 |
| DE602004024813D1 (de) | 2010-02-04 |
| EP1678300A1 (de) | 2006-07-12 |
| EP1678300B1 (de) | 2009-12-23 |
| WO2005045031A1 (fr) | 2005-05-19 |
| FR2861405A1 (fr) | 2005-04-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |