ATE374258T1 - GERMIN EXTRACT FOR CELL-FREE PROTEIN SYNTHESIS AND METHOD FOR THE PRODUCTION THEREOF - Google Patents
GERMIN EXTRACT FOR CELL-FREE PROTEIN SYNTHESIS AND METHOD FOR THE PRODUCTION THEREOFInfo
- Publication number
- ATE374258T1 ATE374258T1 AT03734900T AT03734900T ATE374258T1 AT E374258 T1 ATE374258 T1 AT E374258T1 AT 03734900 T AT03734900 T AT 03734900T AT 03734900 T AT03734900 T AT 03734900T AT E374258 T1 ATE374258 T1 AT E374258T1
- Authority
- AT
- Austria
- Prior art keywords
- cell
- protein synthesis
- free protein
- extract
- germin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention is capable of providing: a method for preparing plant embryo extract wherein a step of mincing a plant embryo is performed by impact or chopping and/or in the presence of extracting solvent; and embryo extract produced by this method, which is low in impurities that are not necessary for, or have a negative impact on, cell-free protein synthesis. The present invention allows for stable, high-efficiency cell-free protein synthesis.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002023138 | 2002-01-31 | ||
JP2002023140 | 2002-01-31 | ||
JP2002023139 | 2002-01-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE374258T1 true ATE374258T1 (en) | 2007-10-15 |
Family
ID=27670280
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT03734900T ATE374258T1 (en) | 2002-01-31 | 2003-01-31 | GERMIN EXTRACT FOR CELL-FREE PROTEIN SYNTHESIS AND METHOD FOR THE PRODUCTION THEREOF |
Country Status (7)
Country | Link |
---|---|
US (1) | US20050042305A1 (en) |
EP (1) | EP1477566B1 (en) |
JP (1) | JP3746780B2 (en) |
AT (1) | ATE374258T1 (en) |
CA (1) | CA2474466A1 (en) |
DE (1) | DE60316541D1 (en) |
WO (1) | WO2003064671A1 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2483332A1 (en) | 2002-01-31 | 2003-08-07 | Yaeta Endo | Cell extract for cell-free protein synthesis and process for producing the same |
ATE493203T1 (en) * | 2003-06-16 | 2011-01-15 | Monsanto Technology Llc | METHOD AND DEVICE FOR PRODUCING GENETICALLY TRANSFORMABLE PLANT TISSUE |
US7150993B2 (en) | 2003-08-05 | 2006-12-19 | Monsanto Technology Llc | Method for excision of plant embryos for transformation |
US7560611B2 (en) | 2003-08-05 | 2009-07-14 | Monsanto Technology Llc | Method and apparatus for substantially isolating plant tissues |
US7435538B2 (en) * | 2003-09-08 | 2008-10-14 | Cellfree Sciences Co., Ltd. | High throughput screening method of drug for physiologically active protein |
WO2006019876A2 (en) * | 2004-07-14 | 2006-02-23 | Invitrogen Corporation | Production of fusion proteins by cell-free protein synthesis |
CN105219694A (en) | 2007-03-09 | 2016-01-06 | 孟山都技术公司 | For the preparation of plant embryo explants that transforms and purposes |
CN110168086B (en) | 2016-11-11 | 2024-01-16 | 住友制药株式会社 | malaria vaccine |
KR20230148370A (en) | 2021-02-25 | 2023-10-24 | 아덴트 밀즈, 엘엘씨 | System and method for extraction and isolation of purified wheat germ products |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69216385T2 (en) * | 1991-10-11 | 1997-06-12 | Promega Corp | COUPLED TRANSCRIPTION AND TRANSLATION IN CELL-FREE EUKARYOT EXTRACTS |
JPH06225783A (en) * | 1992-12-07 | 1994-08-16 | Nippon Flour Mills Co Ltd | Production of protein using cell-free protein synthesis system |
US5693506A (en) * | 1993-11-16 | 1997-12-02 | The Regents Of The University Of California | Process for protein production in plants |
KR0131166B1 (en) * | 1994-05-04 | 1998-04-11 | 최차용 | Preparation process of proteins in a non-cell system |
JP3753358B2 (en) * | 1999-02-24 | 2006-03-08 | 株式会社セルフリーサイエンス | Germ extract for cell-free protein synthesis, method for producing the same, and method for protein synthesis using the same |
WO2000068412A1 (en) * | 1999-05-11 | 2000-11-16 | Wakenyaku Co., Ltd. | Preparation containing cell extract for synthesizing cell-free protein and means for synthesizing cell-free protein |
CN1102022C (en) * | 2000-11-14 | 2003-02-26 | 南京农业大学 | Storage burable rice variety developing method |
CN1511254A (en) * | 2001-05-18 | 2004-07-07 | Զ������̫ | Method of screening germ for cell-free protein synthesis and method of producing germ extract for cell-free protein synthesis |
CA2483332A1 (en) * | 2002-01-31 | 2003-08-07 | Yaeta Endo | Cell extract for cell-free protein synthesis and process for producing the same |
WO2003072796A1 (en) * | 2002-02-28 | 2003-09-04 | Yaeta Endo | Reaction solution for cell-free protein synthesis, method of preparing the same and protein synthesis method using the same |
-
2003
- 2003-01-31 DE DE60316541T patent/DE60316541D1/en not_active Expired - Lifetime
- 2003-01-31 US US10/503,271 patent/US20050042305A1/en not_active Abandoned
- 2003-01-31 CA CA002474466A patent/CA2474466A1/en not_active Abandoned
- 2003-01-31 JP JP2003564262A patent/JP3746780B2/en not_active Expired - Fee Related
- 2003-01-31 WO PCT/JP2003/000995 patent/WO2003064671A1/en active IP Right Grant
- 2003-01-31 AT AT03734900T patent/ATE374258T1/en not_active IP Right Cessation
- 2003-01-31 EP EP03734900A patent/EP1477566B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
WO2003064671A1 (en) | 2003-08-07 |
EP1477566A4 (en) | 2005-06-22 |
JPWO2003064671A1 (en) | 2005-05-26 |
EP1477566A1 (en) | 2004-11-17 |
US20050042305A1 (en) | 2005-02-24 |
EP1477566B1 (en) | 2007-09-26 |
JP3746780B2 (en) | 2006-02-15 |
DE60316541D1 (en) | 2007-11-08 |
CA2474466A1 (en) | 2003-08-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |