ATE371730T1 - Detektionsverfahren umfassend die verwendung von polypeptiden mit einem coiled-coil und einer additionsstelle - Google Patents

Detektionsverfahren umfassend die verwendung von polypeptiden mit einem coiled-coil und einer additionsstelle

Info

Publication number
ATE371730T1
ATE371730T1 AT98940391T AT98940391T ATE371730T1 AT E371730 T1 ATE371730 T1 AT E371730T1 AT 98940391 T AT98940391 T AT 98940391T AT 98940391 T AT98940391 T AT 98940391T AT E371730 T1 ATE371730 T1 AT E371730T1
Authority
AT
Austria
Prior art keywords
polypeptide
polypeptides
methods include
detection methods
coiled coil
Prior art date
Application number
AT98940391T
Other languages
English (en)
Inventor
John Colyer
Joanne Lightowler
Derek Woolfson
Original Assignee
Cyclacel Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cyclacel Ltd filed Critical Cyclacel Ltd
Application granted granted Critical
Publication of ATE371730T1 publication Critical patent/ATE371730T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/91—Fusion polypeptide containing a motif for post-translational modification containing a motif for glycosylation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Inspection Of Paper Currency And Valuable Securities (AREA)
  • Polarising Elements (AREA)
AT98940391T 1997-08-30 1998-09-01 Detektionsverfahren umfassend die verwendung von polypeptiden mit einem coiled-coil und einer additionsstelle ATE371730T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9718358.6A GB9718358D0 (en) 1997-08-30 1997-08-30 Chemical modification

Publications (1)

Publication Number Publication Date
ATE371730T1 true ATE371730T1 (de) 2007-09-15

Family

ID=10818247

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98940391T ATE371730T1 (de) 1997-08-30 1998-09-01 Detektionsverfahren umfassend die verwendung von polypeptiden mit einem coiled-coil und einer additionsstelle

Country Status (11)

Country Link
US (3) US20030027220A1 (de)
EP (1) EP1007653B1 (de)
JP (1) JP2001514849A (de)
AT (1) ATE371730T1 (de)
AU (1) AU8872798A (de)
CA (1) CA2302394A1 (de)
DE (1) DE69838333T2 (de)
GB (2) GB9718358D0 (de)
IL (1) IL134179A0 (de)
NO (1) NO20000901L (de)
WO (1) WO1999011774A1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9904398D0 (en) * 1999-02-25 1999-04-21 Fluorescience Ltd High throughput assay
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
US7166475B2 (en) 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
US7060506B2 (en) * 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
SE0001530D0 (sv) * 2000-04-27 2000-04-27 Accuro Immunology Ab A reporter protein
EP1290215A2 (de) * 2000-06-07 2003-03-12 Cyclacel Limited Verfahren zur beobachtung von enzymaktivität
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
GB0214553D0 (en) * 2002-06-25 2002-08-07 Univ Leeds Reporter module molecules having active ion channel activity
US7858386B2 (en) * 2006-03-07 2010-12-28 The United States Of America As Represented By The Secretary Of The Navy Method of controlling quantum dot photoluminescence and other intrinsic properties through biological specificity
KR20120026838A (ko) * 2010-09-10 2012-03-20 삼성전자주식회사 표적 서열 결합 단백질을 포함하는 키트 및 그를 이용한 표적 핵산의 검출 방법
GB201306634D0 (en) 2013-04-11 2013-05-29 Univ Bristol Cage-like particles

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0537270T3 (da) * 1990-07-02 1999-06-07 Univ California Påvisning af analytter ved overføring af fluorescensenergi
ATE151113T1 (de) * 1992-01-23 1997-04-15 Merck Patent Gmbh Fusionsproteine von monomeren und dimeren von antikörperfragmenten
US5824483A (en) * 1994-05-18 1998-10-20 Pence Inc. Conformationally-restricted combinatiorial library composition and method
JPH11512620A (ja) * 1995-10-06 1999-11-02 ペンス 発現されたタンパク質の検出および精製のためのコイルドコイルヘテロダイマー法および組成物
US6165335A (en) * 1996-04-25 2000-12-26 Pence And Mcgill University Biosensor device and method
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes

Also Published As

Publication number Publication date
DE69838333D1 (de) 2007-10-11
IL134179A0 (en) 2001-04-30
JP2001514849A (ja) 2001-09-18
DE69838333T2 (de) 2007-12-20
EP1007653A1 (de) 2000-06-14
GB2342652A (en) 2000-04-19
US20030027220A1 (en) 2003-02-06
GB2342652B (en) 2002-03-27
CA2302394A1 (en) 1999-03-11
NO20000901L (no) 2000-04-28
GB0001228D0 (en) 2000-03-08
EP1007653B1 (de) 2007-08-29
AU8872798A (en) 1999-03-22
WO1999011774A1 (en) 1999-03-11
NO20000901D0 (no) 2000-02-24
GB9718358D0 (en) 1997-11-05
US20060148017A1 (en) 2006-07-06
US20030104504A1 (en) 2003-06-05

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