ATE28666T1 - Agarose-gel elektrophoretisches verfahren zur bestimmung von amylase-isoenzymen. - Google Patents

Agarose-gel elektrophoretisches verfahren zur bestimmung von amylase-isoenzymen.

Info

Publication number
ATE28666T1
ATE28666T1 AT84301960T AT84301960T ATE28666T1 AT E28666 T1 ATE28666 T1 AT E28666T1 AT 84301960 T AT84301960 T AT 84301960T AT 84301960 T AT84301960 T AT 84301960T AT E28666 T1 ATE28666 T1 AT E28666T1
Authority
AT
Austria
Prior art keywords
sub
agarose gel
gel electrophoretic
electrophoretic method
barbital
Prior art date
Application number
AT84301960T
Other languages
English (en)
Inventor
Vicki Lynn Royse
Donald Milton Jensen
Original Assignee
Rush Presbyterian St Luke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rush Presbyterian St Luke filed Critical Rush Presbyterian St Luke
Application granted granted Critical
Publication of ATE28666T1 publication Critical patent/ATE28666T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/806—Electrical property or magnetic property

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Electrochemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT84301960T 1983-03-23 1984-03-22 Agarose-gel elektrophoretisches verfahren zur bestimmung von amylase-isoenzymen. ATE28666T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/478,506 US4559120A (en) 1983-03-23 1983-03-23 Agarose gel electrophoresis technique for the determination of amylase isoenzymes
EP84301960A EP0122742B1 (de) 1983-03-23 1984-03-22 Agarose-Gel elektrophoretisches Verfahren zur Bestimmung von Amylase-Isoenzymen

Publications (1)

Publication Number Publication Date
ATE28666T1 true ATE28666T1 (de) 1987-08-15

Family

ID=23900234

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84301960T ATE28666T1 (de) 1983-03-23 1984-03-22 Agarose-gel elektrophoretisches verfahren zur bestimmung von amylase-isoenzymen.

Country Status (5)

Country Link
US (1) US4559120A (de)
EP (1) EP0122742B1 (de)
AT (1) ATE28666T1 (de)
CA (1) CA1210676A (de)
DE (1) DE3465112D1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4668363A (en) * 1984-03-16 1987-05-26 Beckman Instruments, Inc. Immunofixation electrophoresis process
GB8428876D0 (en) * 1984-11-15 1984-12-27 Ciba Geigy Ag Diagnostic strips
JPS6266153A (ja) * 1985-09-18 1987-03-25 Fuji Photo Film Co Ltd 電気泳動用媒体
DK0454763T3 (da) * 1989-01-13 1996-11-25 Fmc Corp Adskillelsesgeler og gelsystemer på polysaccharidbasis til stabelelektroforese og fremgangsmåde til støbning af en elektroforesegel
US5355304A (en) * 1990-01-30 1994-10-11 Demoranville Victoria E Clinical laboratory work-flow system which semi-automates validated immunoassay and electrophoresis protocols
US5202006A (en) * 1992-04-17 1993-04-13 Beckman Instruments, Inc. Analysis of hemoglobin variants by capillary zone electrophoresis
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
KR100884430B1 (ko) * 2005-09-14 2009-02-19 파나소닉 전공 주식회사 메모리 카드 유지 구조체

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA870180A (en) * 1971-05-04 L. Louderback Allan Method of electrophoresis
US3527712A (en) * 1967-03-07 1970-09-08 Marine Colloids Inc Dried agarose gel,method of preparation thereof,and production of aqueous agarose gel
US3497437A (en) * 1967-06-21 1970-02-24 Baxter Laboratories Inc Method of electrophoresis
US3687833A (en) * 1969-03-10 1972-08-29 Upjohn Co Purification of lactogen
JPS5244378B2 (de) * 1973-05-22 1977-11-08
US3912610A (en) * 1974-07-23 1975-10-14 Icl Scient Method for electroquantitative determination of proteins
US4030995A (en) * 1976-07-13 1977-06-21 Sigma Chemical Company Alkaline phosphatase isoenzyme determination
SU681362A1 (ru) * 1978-01-31 1979-08-25 Институт Ботаники Ан Казахской Сср Способ электрофоретического разделени изоферментов -амилазы
US4246084A (en) * 1979-05-29 1981-01-20 Beckman Instruments, Inc. Electrophoretic technique for assaying the relative distribution of lactate dehydrogenase isoenztimes and buffers for use therein
US4209372A (en) * 1979-06-01 1980-06-24 Corning Glass Works Alkaline agarosse gel electrophoresis of hemoglobins
US4454161A (en) * 1981-02-07 1984-06-12 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Process for the production of branching enzyme, and a method for improving the qualities of food products therewith

Also Published As

Publication number Publication date
EP0122742A2 (de) 1984-10-24
CA1210676A (en) 1986-09-02
DE3465112D1 (en) 1987-09-03
EP0122742A3 (en) 1986-03-12
US4559120A (en) 1985-12-17
EP0122742B1 (de) 1987-07-29

Similar Documents

Publication Publication Date Title
Seidel et al. Improved techniques for assessment of plasma lipoprotein patterns. I. Precipitation in gels after electrophoresis with polyanionic compounds
Robertson et al. Rapid isoelectric focusing in a vertical polyacrylamide minigel system
Westermeier et al. High-resolution two-dimensional electrophoresis with isoelectric focusing in immobilized pH gradients
EP0174990B2 (de) Verbessertes verfahren zur immunofixierungselektrophorese
Kitchin Demonstration of the inherited serum group specific protein by acrylamide electrophoresis.
Yao et al. Determination of isoelectric points of acidic and basic proteins by capillary electrophoresis
ATE28666T1 (de) Agarose-gel elektrophoretisches verfahren zur bestimmung von amylase-isoenzymen.
ATE69108T1 (de) Elektrophoretisches gel hoher aufloesung sowie methode zur abscheidung von serumproteinen.
Wallace et al. Visualization of protein—SDS complexes in polyacrylamide gels by chilling
Arnaud et al. The Pi system: its study by means of thin-layer-gel electrofocusing in polyacrylamide gel
Corradini et al. N, N, N′, N′‐Tetramethyl‐1, 3‐butanediamine as effective running electrolyte additive for efficient electrophoretic separation of basic proteins in bare fused‐silica capillaries
ES2101808T3 (es) Cuantificacion de muestras utilizando electroforesis capilar.
Boettcher et al. Electrophoresis of human salivary amylase in gel slabs
SE8205388L (sv) Forbettrad elektroforetisk metod for separering av serumproteiner och forbettrad elektroforetisk gel for anvendning dertill
Toda et al. Two-dimensional electrophoresis on the layers of cellulose acetate membrane
Kamboh et al. A sensitive immunoblotting technique to identify thyroxin-binding globulin protein heterogeneity after isoelectric focusing
Stoklosa et al. Molecular weight determinations of proteins by polyacrylamide gel electrophoresis with sodium dodecyl sulfate in just the sample solution
Manabe et al. Identification of bovine fetal and adult serum/plasma proteins by two‐dimensional electrophoresis and immunochemical staining
US5171410A (en) Method and formulation for creating kinase isoform separation
Bhavsar et al. A method to increase efficiency and minimize anomalous electrophoretic transfer in protein blotting
Karinkanta et al. An improved technique for immunofixation of electrophoretograms.
Felberg et al. Myeloperoxidase: VII. Separation of oppositely charged proteins or subunits by disc electrophoresis
Gonzalez et al. 2‐D native‐PAGE/SDS‐PAGE visualization of an oligomer's subunits: Application to the analysis of IgG
Annadurai et al. High resolution two‐dimensional mapping of tissue‐specific polypeptides in the desert locust, Schistocerca gregaria
Archibald Further studies on bovine serum amylase. 2. Affinity electrophoresis

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee