ATE225856T1 - Verfahren zur koamplifikation zweier unterschiedlicher nukleinsäure-sequenzen unter verwendung der polymerase-kettenreaktion - Google Patents

Verfahren zur koamplifikation zweier unterschiedlicher nukleinsäure-sequenzen unter verwendung der polymerase-kettenreaktion

Info

Publication number
ATE225856T1
ATE225856T1 AT94201913T AT94201913T ATE225856T1 AT E225856 T1 ATE225856 T1 AT E225856T1 AT 94201913 T AT94201913 T AT 94201913T AT 94201913 T AT94201913 T AT 94201913T AT E225856 T1 ATE225856 T1 AT E225856T1
Authority
AT
Austria
Prior art keywords
acid sequences
nucleic acid
chain reaction
polymerase chain
coamplification
Prior art date
Application number
AT94201913T
Other languages
English (en)
Inventor
John W Backus
William Harold Donish
John Bruce Findlay
John W H Sutherland
Marlene M King
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Application granted granted Critical
Publication of ATE225856T1 publication Critical patent/ATE225856T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT94201913T 1993-07-08 1994-07-02 Verfahren zur koamplifikation zweier unterschiedlicher nukleinsäure-sequenzen unter verwendung der polymerase-kettenreaktion ATE225856T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US8998793A 1993-07-08 1993-07-08

Publications (1)

Publication Number Publication Date
ATE225856T1 true ATE225856T1 (de) 2002-10-15

Family

ID=22220547

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94201913T ATE225856T1 (de) 1993-07-08 1994-07-02 Verfahren zur koamplifikation zweier unterschiedlicher nukleinsäure-sequenzen unter verwendung der polymerase-kettenreaktion

Country Status (7)

Country Link
US (1) US5674717A (de)
EP (1) EP0648845B1 (de)
JP (1) JP4047395B2 (de)
AT (1) ATE225856T1 (de)
DE (1) DE69431510T2 (de)
DK (1) DK0648845T3 (de)
SG (1) SG68568A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5559013A (en) * 1994-06-22 1996-09-24 Johnson & Johnson Clinical Diagnostics, Inc. Method of amplification using intermediate renaturation step
US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
CA2221454A1 (en) * 1995-05-19 1996-11-21 Abbott Laboratories Wide dynamic range nucleic acid detection using an aggregate primer series
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
WO1998011259A2 (en) * 1996-09-09 1998-03-19 Visible Genetics Inc. Composition, method and kit for detection and identification of sexually transmitted disease microorganisms
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
US6268136B1 (en) 1997-06-16 2001-07-31 Exact Science Corporation Methods for stool sample preparation
US6333179B1 (en) 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
CN1167794C (zh) 1998-09-08 2004-09-22 宝酒造株式会社 Dna的合成方法
WO2000032820A1 (en) * 1998-11-23 2000-06-08 Exact Laboratories, Inc. Method for detecting target sequences in small proportions in heterogeneous samples
JP2002537777A (ja) 1999-02-25 2002-11-12 エグザクト サイエンシーズ コーポレイション Dnaの完全性を保存するための方法
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
AU4714201A (en) 1999-12-07 2001-06-18 Exact Sciences Corporation Supracolonic aerodigestive neoplasm detection
GB0114639D0 (en) * 2001-06-15 2001-08-08 Sec Dep Of The Home Department Improvements in and relating to analysis of DNA
WO2003071252A2 (en) 2002-02-15 2003-08-28 Exact Sciences Corporation Methods for analysis of molecular events
AU2007233252C1 (en) 2006-03-29 2013-05-23 Boehringer Ingelheim Animal Health USA Inc. Vaccine against Streptococci
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
EP3353325B1 (de) * 2015-09-24 2024-03-20 AbVitro LLC Single-amplikon-aktivierte ausschluss-pcr

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5008182A (en) * 1986-01-10 1991-04-16 Cetus Corporation Detection of AIDS associated virus by polymerase chain reaction
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5176995A (en) * 1985-03-28 1993-01-05 Hoffmann-La Roche Inc. Detection of viruses by amplification and hybridization
US5079351A (en) * 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5389512A (en) * 1988-10-07 1995-02-14 Hoffman-La Roche Inc. Method for determining the relative amount of a viral nucleic acid segment in a sample by the polymerase chain reaction
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
CA2065719A1 (en) * 1991-04-30 1992-10-31 John B. Findlay Nucleic acid amplification and detection methods using rapid polymerase chain reaction cycle
SG63589A1 (en) * 1993-05-14 1999-03-30 Johnson & Johnson Clin Diag Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures

Also Published As

Publication number Publication date
EP0648845B1 (de) 2002-10-09
SG68568A1 (en) 1999-11-16
DE69431510D1 (de) 2002-11-14
DK0648845T3 (da) 2003-02-17
US5674717A (en) 1997-10-07
EP0648845A3 (de) 1995-09-06
JPH07163370A (ja) 1995-06-27
DE69431510T2 (de) 2003-02-27
JP4047395B2 (ja) 2008-02-13
EP0648845A2 (de) 1995-04-19

Similar Documents

Publication Publication Date Title
ATE225856T1 (de) Verfahren zur koamplifikation zweier unterschiedlicher nukleinsäure-sequenzen unter verwendung der polymerase-kettenreaktion
ATE220726T1 (de) Oligonukleotid-primer für amplifizierung von hcv- nukleinsäure
DE69224548D1 (de) Verfahren zur Amplifizierung und zum Nachweis von Nukleinsäuren,die einen schnellen PCR-Zyklus verwenden
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
ATE83505T1 (de) Verfahren zur amplifikation von nukleinsaeuresequenzen.
SG96532A1 (en) Diagnostics and amplification methods overcoming a primer-target mismatch at the primer 3'end
ATE266102T1 (de) Verfahren zur koamplifikation einer zielnukleinsäure unter versendung eines volumenabscheidungsmittel in der reaktionszusammensetzung, testsatz und testvorrichtung
DE69428167D1 (de) Reagenz und Verfahren für reverse Transkription bei hoher Temperatur, verbunden mit einer Polymerase-Kettenreaktion
ATE398186T1 (de) Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren
AU650055B2 (en) Nucleotide sequences useful as type-specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods
ATE360098T1 (de) Nachweis und differenzierung von mikroorganismen der spezies yersinia pestis/yersinia pseudotuberculosis
DE602004028993D1 (de) Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion
ATE197481T1 (de) Verfahren und reagenzien zur typisierung von hla- genen der klasse i
ATE456669T1 (de) Verfahren zur quantifizierung von nukleinsäuren
DE60015210D1 (de) Oligonukleotid Primer zur effizienten reversen Transkription von Hepatitis C Virus (HCV) RNS und Verfahren zu deren Verwendung
ATE250672T1 (de) Verfahren zum nachweis der aktivität von dns polymerasen

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification