ATE156909T1 - Färbeverfahren mittels säurefarbstoffe - Google Patents
Färbeverfahren mittels säurefarbstoffeInfo
- Publication number
- ATE156909T1 ATE156909T1 AT92305709T AT92305709T ATE156909T1 AT E156909 T1 ATE156909 T1 AT E156909T1 AT 92305709 T AT92305709 T AT 92305709T AT 92305709 T AT92305709 T AT 92305709T AT E156909 T1 ATE156909 T1 AT E156909T1
- Authority
- AT
- Austria
- Prior art keywords
- acid
- staining
- dyeing process
- acid dyes
- supporting matrix
- Prior art date
Links
- 239000000980 acid dye Substances 0.000 title abstract 3
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical compound [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 title 1
- 238000004043 dyeing Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000011159 matrix material Substances 0.000 abstract 2
- 238000010186 staining Methods 0.000 abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 150000007524 organic acids Chemical class 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000007447 staining method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Compositions Of Macromolecular Compounds (AREA)
- Coloring (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17878891 | 1991-06-24 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE156909T1 true ATE156909T1 (de) | 1997-08-15 |
Family
ID=16054654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT92305709T ATE156909T1 (de) | 1991-06-24 | 1992-06-22 | Färbeverfahren mittels säurefarbstoffe |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5284560A (de) |
| EP (1) | EP0520725B1 (de) |
| JP (1) | JP2803475B2 (de) |
| AT (1) | ATE156909T1 (de) |
| CA (1) | CA2071736A1 (de) |
| DE (1) | DE69221550T2 (de) |
| DK (1) | DK0520725T3 (de) |
| ES (1) | ES2104835T3 (de) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5567626A (en) * | 1995-04-11 | 1996-10-22 | Pall Corporation | Method of detecting biological materials using a polyvinyllidene fluoride membrane |
| US6319720B1 (en) * | 1998-10-13 | 2001-11-20 | Ewald M. Wondrak | Process for fast visualization of protein |
| DE10021390C2 (de) * | 2000-05-03 | 2002-06-27 | Juergen Olert | Protektionslösung und Fixierverfahren für die Paraffinschnitt-Technik |
| JP4751605B2 (ja) * | 2004-12-14 | 2011-08-17 | 株式会社アドバンス | 電気泳動用染色液 |
| CN101936837B (zh) * | 2009-06-26 | 2012-09-05 | 南京金斯瑞生物科技有限公司 | 生物聚合物快速染色法 |
| JP5750768B2 (ja) * | 2012-01-25 | 2015-07-22 | 国立研究開発法人産業技術総合研究所 | 糖タンパク質又は多糖類の検出方法 |
| CN104004382B (zh) * | 2014-05-20 | 2016-01-20 | 北京五康新兴科技有限公司 | 一种考马斯亮蓝染色液和染色方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU561898A1 (ru) * | 1974-12-09 | 1977-06-15 | Всесоюзный Научно-Исследовательский Институт Мясной Промышленности | Способ электрофоретического анализа белков |
| US4023933A (en) * | 1976-06-10 | 1977-05-17 | The University Of Georgia | Protein-assay reagent and method |
| JPS6049256B2 (ja) * | 1979-11-09 | 1985-10-31 | 大塚製薬株式会社 | 蛋白質分析用詩薬 |
| JPS56103366A (en) * | 1980-01-21 | 1981-08-18 | Otsuka Pharmaceut Co Ltd | Reagent for protein analysis and quantitative method of protein |
| SU951121A1 (ru) * | 1980-10-29 | 1982-08-15 | Институт химии им.В.И.Никитина | Способ вы влени белков |
| US4405720A (en) * | 1981-03-04 | 1983-09-20 | The United States Of America As Represented By The Department Of Health And Human Services | Silver stains for protein in gels |
| US5064768A (en) * | 1988-09-16 | 1991-11-12 | Daiichi Pure Chemicals Co., Ltd. | Silver staining technique and kit |
-
1992
- 1992-06-19 CA CA002071736A patent/CA2071736A1/en not_active Abandoned
- 1992-06-22 ES ES92305709T patent/ES2104835T3/es not_active Expired - Lifetime
- 1992-06-22 DE DE69221550T patent/DE69221550T2/de not_active Expired - Fee Related
- 1992-06-22 AT AT92305709T patent/ATE156909T1/de not_active IP Right Cessation
- 1992-06-22 DK DK92305709.5T patent/DK0520725T3/da active
- 1992-06-22 EP EP92305709A patent/EP0520725B1/de not_active Expired - Lifetime
- 1992-06-23 JP JP4188707A patent/JP2803475B2/ja not_active Expired - Lifetime
- 1992-06-23 US US07/902,626 patent/US5284560A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2104835T3 (es) | 1997-10-16 |
| EP0520725A3 (de) | 1994-03-09 |
| DK0520725T3 (da) | 1997-09-22 |
| CA2071736A1 (en) | 1992-12-25 |
| US5284560A (en) | 1994-02-08 |
| JPH05281190A (ja) | 1993-10-29 |
| EP0520725B1 (de) | 1997-08-13 |
| JP2803475B2 (ja) | 1998-09-24 |
| DE69221550T2 (de) | 1998-02-19 |
| EP0520725A2 (de) | 1992-12-30 |
| DE69221550D1 (de) | 1997-09-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |