ATE121778T1 - EXPRESSION AND EXCRETION VECTORS FOR HIRUDIN USING TRANSFORMED YEAST. - Google Patents

EXPRESSION AND EXCRETION VECTORS FOR HIRUDIN USING TRANSFORMED YEAST.

Info

Publication number
ATE121778T1
ATE121778T1 AT86400961T AT86400961T ATE121778T1 AT E121778 T1 ATE121778 T1 AT E121778T1 AT 86400961 T AT86400961 T AT 86400961T AT 86400961 T AT86400961 T AT 86400961T AT E121778 T1 ATE121778 T1 AT E121778T1
Authority
AT
Austria
Prior art keywords
hirudin
expression
transformed yeast
excretion
vectors
Prior art date
Application number
AT86400961T
Other languages
German (de)
Inventor
Gerard Loison
Paul Tolstoshev
Street
Yves Lemoine
Jean-Pierre Lecocq
Original Assignee
Transgene Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgene Sa filed Critical Transgene Sa
Application granted granted Critical
Publication of ATE121778T1 publication Critical patent/ATE121778T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Instructional Devices (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Glass Compositions (AREA)

Abstract

Functional block of DNA enabling to prepare hirudine from yeast, characterized in that it comprises at least: - the gene of hirudine or one of its variants (gene H); - A DNA sequence (Str) comprising the signals providing for the transcription of the gene H by the yeast.
AT86400961T 1985-05-02 1986-05-02 EXPRESSION AND EXCRETION VECTORS FOR HIRUDIN USING TRANSFORMED YEAST. ATE121778T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR8506672A FR2593518B1 (en) 1985-05-02 1985-05-02 VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS

Publications (1)

Publication Number Publication Date
ATE121778T1 true ATE121778T1 (en) 1995-05-15

Family

ID=9318888

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86400961T ATE121778T1 (en) 1985-05-02 1986-05-02 EXPRESSION AND EXCRETION VECTORS FOR HIRUDIN USING TRANSFORMED YEAST.

Country Status (23)

Country Link
EP (1) EP0200655B1 (en)
JP (2) JP2580141B2 (en)
KR (1) KR870700234A (en)
AT (1) ATE121778T1 (en)
AU (1) AU600667B2 (en)
BG (1) BG49717A3 (en)
CA (1) CA1341501C (en)
CZ (1) CZ282928B6 (en)
DE (1) DE3650306T2 (en)
DK (1) DK174837B1 (en)
ES (1) ES8703930A1 (en)
FI (1) FI104635B (en)
FR (2) FR2593518B1 (en)
HK (1) HK1006183A1 (en)
HU (1) HU202919B (en)
IE (1) IE67136B1 (en)
MC (1) MC1785A1 (en)
NO (1) NO302375B1 (en)
PL (1) PL155074B1 (en)
PT (1) PT82490B (en)
RU (1) RU1774950C (en)
WO (1) WO1986006406A1 (en)
ZA (1) ZA863261B (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (en) * 1987-11-13 1989-05-24 Hoechst Ag METHOD FOR INSULATING AND CLEANING HIRUDINE
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
FR2607516B2 (en) * 1986-12-01 1989-12-22 Transgene Sa IMPROVED VECTORS FOR EXPRESSING A HIRUDIN VARIANT IN YEAST, PROCESS AND PRODUCT OBTAINED
MC1834A1 (en) * 1986-07-10 1988-06-03 Transgene Sa DNA FUNCTIONAL BLOCK AND PLASMID ENCODING HIRUDINE, TRANSFORMED YEAST, PROCESS FOR PREPARING HIRUDINE, HIRUDINE OBTAINED AND ITS USE
FR2607517B2 (en) * 1986-12-01 1989-12-22 Transgene Sa VECTORS FOR EXPRESSING HIRUDIN VARIANTS IN YEAST, PROCESS AND PRODUCT OBTAINED
FR2611723B1 (en) * 1987-02-27 1989-09-08 Transgene Sa RECOMBINANT DNA EXPRESSION VECTOR ENCODING HIRUDINE, INFECTED OR TRANSFORMED MAMMALIAN CELL CULTURES, PROCESS FOR PREPARING HIRUDINE AND PROTEIN OBTAINED
JPH03503596A (en) * 1987-06-24 1991-08-15 ノボ・ノルディスク　エー／エス A method for preparing a protein or polypeptide, a DNA sequence encoding the polypeptide, a microorganism having the DNA sequence and the polypeptide, and use of the polypeptide as a pharmaceutical preparation
US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
DE3819079A1 (en) * 1988-06-04 1989-12-07 Hoechst Ag HIRUDINE DERIVATIVES WITH DELAYED EFFECT
DE68925893T2 (en) * 1988-07-23 1996-08-08 Delta Biotechnology Ltd SECRETORIC LEADER SEQUENCES
DE3835815A1 (en) * 1988-10-21 1990-04-26 Hoechst Ag NEW ISOHIRUDINE
CA2000887A1 (en) * 1988-11-01 1990-05-01 Cecilia S.L. Ku Thromboresistant materials and methods for making same
US5053453A (en) * 1988-11-01 1991-10-01 Baxter International Inc. Thromboresistant materials and methods for making same
FR2646437B1 (en) * 1989-04-28 1991-08-30 Transgene Sa NOVEL DNA SEQUENCES, THEIR APPLICATION AS A SEQUENCE ENCODING A SIGNAL PEPTIDE FOR THE SECRETION OF MATURE PROTEINS BY RECOMBINANT YEASTS, EXPRESSION CASSETTES, PROCESSED YEASTS AND PROCESS FOR PREPARING THE SAME
DE4009268A1 (en) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind SECRETION OF HIRUDIN DERIVATIVES
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
US5837808A (en) * 1991-08-20 1998-11-17 Baxter International Inc. Analogs of hirudin
DE4140381A1 (en) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De NEW SYNTHETIC ISOHIRUDINE WITH IMPROVED STABILITY
ATE196927T1 (en) * 1992-09-04 2000-10-15 Novartis Ag METHOD FOR PRODUCING PROTEASE INHIBITORS
IL114160A (en) * 1994-06-17 2006-12-31 Novo Nordisk As Dna constructs encoding heterologous proteins and processes for the heterologous protein production in yeast
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
DE19529997C1 (en) 1995-08-16 1997-04-10 Hoechst Ag A method for inactivating carboxypeptidase Y in hirudin-containing culture broths
DE19543737A1 (en) 1995-11-24 1997-05-28 Hoechst Ag Process for the ultrafiltration of biological matrices containing peptides or proteins
DE19544233A1 (en) * 1995-11-28 1997-06-05 Hoechst Ag Process for using the yeast ADH II promoter system for the biotechnological production of heterologous proteins in high yields
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
EP0787569B1 (en) 1996-01-31 2002-10-02 Sumitomo Bakelite Company Limited Method of producing epoxy resin-encapsulated semiconductor device
US6265204B1 (en) 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10149678A1 (en) 2001-10-09 2009-03-12 Novel Science International Gmbh New peptide compounds containing upto 6 aminoacid residues, are potent and specific thrombin inhibitors used for treating and diagnosing diseases associated with thrombin
DE10301255A1 (en) 2003-01-15 2004-07-29 Cpi Creative Pharma International Gmbh Organic compounds with biological action as thrombin inhibitors and their use
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3382547D1 (en) * 1983-01-12 1992-05-27 Chiron Corp SECRETORIC EXPRESSION IN EUKARYOTS.
AU572125B2 (en) * 1983-03-17 1988-05-05 Mabco Limited Monoclonal antibodies with specificity for crosslinked fibrin and their diagnotic uses
WO1984004330A1 (en) * 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
JPS6041487A (en) * 1983-04-25 1985-03-05 ジエネンテツク・インコ−ポレイテツド Use of alpha factor arrangement in yeast development system
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
JPS59227899A (en) * 1983-05-25 1984-12-21 チロン・コ−ポレイシヨン Manufacture of growth hormone release factor
EP0158564B1 (en) * 1984-03-27 1992-07-15 Transgene S.A. Expression vectors for hirudin, transformed cells and process for the preparation of hirudin
DE3583361D1 (en) * 1984-06-14 1991-08-08 Ucp Gen Pharma Ag METHOD FOR PRODUCING THROMBIN INHIBITORS.
DE3429430A1 (en) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt GENE TECHNOLOGICAL METHOD FOR PRODUCING HIRUDINE AND MEANS FOR IMPLEMENTING THIS METHOD
DE3445517C2 (en) * 1984-12-13 1993-11-18 Ciba Geigy DNA sequence coding for a hirudin-like protein and method for producing a hirudin-like protein
FR2593518B1 (en) * 1985-05-02 1989-09-08 Transgene Sa VECTORS FOR THE EXPRESSION AND SECRETION OF HIRUDIN BY TRANSFORMED YEASTS

Also Published As

Publication number Publication date
HU202919B (en) 1991-04-29
PT82490B (en) 1988-03-03
DE3650306T2 (en) 1995-10-12
CZ282928B6 (en) 1997-11-12
EP0200655B1 (en) 1995-04-26
ES8703930A1 (en) 1987-03-16
FI865303A0 (en) 1986-12-23
IE67136B1 (en) 1996-03-06
PL155074B1 (en) 1991-10-31
CZ321786A3 (en) 1997-07-16
DK634386A (en) 1986-12-30
DK634386D0 (en) 1986-12-30
NO302375B1 (en) 1998-02-23
FR2601383A2 (en) 1988-01-15
FI865303A (en) 1986-12-23
FR2593518B1 (en) 1989-09-08
FR2593518A1 (en) 1987-07-31
DK174837B1 (en) 2003-12-15
RU1774950C (en) 1992-11-07
FI104635B (en) 2000-03-15
NO865336L (en) 1987-03-02
BG49717A3 (en) 1992-01-15
FR2601383B2 (en) 1989-12-22
JPS62502661A (en) 1987-10-15
AU5778786A (en) 1986-11-18
EP0200655A1 (en) 1986-11-05
WO1986006406A1 (en) 1986-11-06
CA1341501C (en) 2006-03-14
AU600667B2 (en) 1990-08-23
JPH09103295A (en) 1997-04-22
MC1785A1 (en) 1987-09-02
HUT42131A (en) 1987-06-29
JP2779930B2 (en) 1998-07-23
KR870700234A (en) 1987-05-30
HK1006183A1 (en) 1999-02-12
DE3650306D1 (en) 1995-06-01
JP2580141B2 (en) 1997-02-12
ES555121A0 (en) 1987-03-16
PT82490A (en) 1986-05-01
ZA863261B (en) 1986-12-30
IE861183L (en) 1986-11-02

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EEIH Change in the person of patent owner
EEIH Change in the person of patent owner
EELA Cancelled due to lapse of time