AT506827A1 - Determining water quality, comprises measuring or evaluating migration speed of positive phototrophic water microbes such as daphnia, euglena, Triops, ciliate and Artemia salina-nauplii and -crayfish, which are attracted using light source - Google Patents
Determining water quality, comprises measuring or evaluating migration speed of positive phototrophic water microbes such as daphnia, euglena, Triops, ciliate and Artemia salina-nauplii and -crayfish, which are attracted using light source Download PDFInfo
- Publication number
- AT506827A1 AT506827A1 AT7872008A AT7872008A AT506827A1 AT 506827 A1 AT506827 A1 AT 506827A1 AT 7872008 A AT7872008 A AT 7872008A AT 7872008 A AT7872008 A AT 7872008A AT 506827 A1 AT506827 A1 AT 506827A1
- Authority
- AT
- Austria
- Prior art keywords
- microbes
- daphnia
- phototrophic
- triops
- light source
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
16/05 2008 16:19 FAX 07722 83890 225 HIBLA BRAUNAU @007 • ···· ·16/05 2008 16:19 FAX 07722 83890 225 HIBLA BRAUNA @ 007 • ···· ·
Beschreibung^Description ^
Die Erfindung bezieht sich auf ein Verfahren, mit dem der Einfluss von physikalischen und chemischen Faktoren auf das physische Verhalten von Kleinlebewesen dadurch ermittelt werden kann, dass man die Bewegungsgeschwindigkeit (Wanderzeit) über eine bestimmte Wegstrecke ermittelt. Die Untersuchungen von Verhaltensmustem verschiedener Testorganismen als Beispiel die Daphnia Magna Straus nach der ISO-Norm 10706 (Water quaiity - Determination of long tcnn toxicity of substaces to Daphnia Magna Straus) wurde erst in jüngerer Zeit als EU-konforme Testmethode anerkannt und etabliert. Eigene Erfahrungen haben aber gezeigt, dass die Durchführung sehr zeitaufwendig ist und keine sehr eindeutigen monokausale Aussagen zu lässt. Im Zusammenhang mit der REACH-Verordnung der EU, bei der es um die toxikologische Neubewertung von chemischen Stoffen geht, ist es von größter Wichtigkeit, schnelle und aussagekräftige Testverfahren zur Verfügung zu haben. Unsere Erfindung besteht aus einer mit wässerigen Medien gefüllten röhrenförmigen Tunnelwanderstrecke, an deren Anfang eine mechanische Vorrichtung zur Freigabe der Wanderstrecke an deren Ende eine optoelektronische Vorrichtung zur Beobachtung mittels ständiger BUddatenaufzeichnung mit einer variablen Lichtquelle vorhanden sind.The invention relates to a method by which the influence of physical and chemical factors on the physical behavior of microorganisms can be determined by determining the speed of movement (time of migration) over a certain distance. The study of behavior patterns of various test organisms as an example of the Daphnia Magna Straus according to the ISO standard 10706 (Water quaiity - Determination of long toxicity of the substance to Daphnia Magna Straus) was only recently recognized and established as an EU-compliant test method. Our own experience has shown, however, that the implementation is very time-consuming and does not allow very clear monocausal statements. In the context of the EU's REACH regulation, which deals with the toxicological reassessment of chemical substances, it is of paramount importance to have quick and meaningful test procedures available. Our invention consists of a tunneled tubular tunnel filled with aqueous media, at the beginning of which there is provided a mechanical device for releasing the traveling path at the end of which an opto-electronic device for observation by means of continuous data acquisition with a variable light source.
Die unter definierten Bedingungen gezüchteten, Licht suchenden Kleinlebewesen (Phototaxis) werden mit physikalischen Methoden wie Temperatur, Schall, Licht und chemischen Stoffen, die über Kläranlagen in öffentliche Gewässer gelangen, nach einem bestimmten Programm unter Stress gesetzt und in die erfindungsgemäße Vorrichtung so eingebracht, dass gleichzeitig mehrere Versuchsorganismen zur gleichen Zeit von einer Art Startvorrichtung zum beleuchteten Ziel am Ende des Tunnels, wo sich auch die Kamera mit Bildauswertung zur Ankunftskonirolle Zeitnehmung befinden, schwimmen können.The light-seeking microorganisms (phototaxis) bred under defined conditions are put under stress according to a specific program using physical methods such as temperature, sound, light and chemical substances that reach public waters via sewage treatment plants and introduced into the device according to the invention such that at the same time several experimental organisms at the same time from a kind of starting device to the illuminated target at the end of the tunnel, where the camera with image analysis to the arrival Konche Zeitzeit are swimming.
Das eigentliche Messverfahren besteht aus den Anlagenteilen Startblock, röhrenförmige Tunnelwanderstrecke, einem starbförmigen, Loch tragenden Zielrundblock mit Beleuchtung und Oberwachungskamera.The actual measurement procedure consists of the plant sections start block, tubular tunnel migration route, a starburst-shaped, hole-bearing target round block with illumination and surveillance camera.
Im Folgenden wird die Erfindung an Hand der Figuren dargestellt.In the following the invention will be illustrated with reference to the figures.
Es zeigen Fig. 1 die Gesammtübersicht, Fig. 2 den Startblock, Fig, 3 die Wanderstrecke, Fig. 4 den Zielblock und Fig. 5 den Trägerring.1 shows the overall overview, FIG. 2 shows the starting block, FIG. 3 shows the traveling distance, FIG. 4 shows the target block and FIG. 5 shows the carrier ring.
Der Startblock (Fig. 2) besteht aus Kunststoff wie Polyethen, Polypropen, PVC oder Polyamid bzw. Keramik oder Metall und enthält eine horizontale Bohrung zum Anstecken des Wanderrohres und zwei vertikale Bohröfihungen, von denen eine die Offiiungs-bzw. Verschlussmechanismen trägt, die die Wanderrohre für die Kleinlebewesen öffnet oder schließt und die andere den Voiraum darstellt, wo die Testorganismen eingebracht werden. Der Öffnungsmechanismus für jedes Wandeirohr enthält einen Stempel aus ferromagnetischem Stahl, der über Magnetspulen gehoben oder gesenkt werden kann, wobei dieser Schritt - hündisch oder elektronisch gesteuert- für alle Startblöcke gleichzeitig ausgelöst werden kann.The starting block (Fig. 2) is made of plastic such as polyethene, polypropene, PVC or polyamide or ceramic or metal and includes a horizontal bore for attaching the walking tube and two vertical Bohröfihungen, one of the opening or. Includes closure mechanisms that open or close the walking tubes for the micro-organisms and the other represents the cavity where the test organisms are introduced. The opening mechanism for each wall pipe contains a ferromagnetic steel stamp which can be raised or lowered by means of magnetic coils, this step being triggered by all or part of the starting blocks, whether manually or electronically controlled.
Die rohrförmige Wanderstrecke (Fig. 3) aus handelsüblichem, lichtundurchlässigem Thermoplast kann bezüglich Innendurchmesser und Länge auf die Größe und die Art der Testlebewesen abgestimmt werden und verbindet flüssigkeitsdicht den Starblock (Fig. 2) mit dem Zielblock (Fig.4 ), der aus transparentem Kunststoff wie Plexiglas, PVC oder aus Glas bestehen kann.The tubular traveling distance (FIG. 3) made of commercially available, opaque thermoplastic material can be matched to the size and type of the test organisms with respect to their inner diameter and length and connects the starblock block (FIG. 2) with the target block (FIG. 4), which is made of transparent material Plastic such as plexiglass, PVC or glass may exist.
Die zum Anlocken der positiv phototrophen (photophilen, Phototaxie) Kleinlebewesen verwendeten, verschiedenfarbigen Lichtquellen sind als LEDs in einem Trägerring aus Kunststoff oder Aluminium so um den kreisförmigen Zielblock justiert, dass in alle Wanderröhren gleichermaßen Licht einfallen kann (Fig, 5), 16/05 2008 FR 17:07 [SE/EM NR 7171] @007 16/05 2008 16:20 FAX 07722 83690 225 HTBLA BRAUNAU @008 ·· • · ···· • ·· • · • · • • · • · • · ··· • · ·· • · • · • ···· · • · • · • • · ·· ·· • · · • ··The different colored light sources used for attracting the positively phototrophic (photophilic, phototaxy) microorganisms are adjusted as LEDs in a carrier ring made of plastic or aluminum around the circular target block so that light can be incident in all traveling tubes equally (Fig. 5), 16/05 2008 FR 17:07 [SE / EM NR 7171] @ 007 16/05 2008 16:20 FAX 07722 83690 225 HTBLA BRAUNA @ 008 ··· · ···· • ··························· ······································································
Die elektronische hochauflösende Kamera beobachtet nach dem Start der Testtiere den Zielraum derartig, dass gleichzeitig alle möglichen sechs Zieleinläufe überwacht werden und ein speziell entwickeltes Bildvergleichsprogranun sofort erkennt, wann ein Testorganismus die Ziellinie überschritten hat Automatisch kann nun die Laufzeit eingelesen und für Auswertungszwecke abgespeichert werden.After starting the test animals, the electronic high-resolution camera observes the target area in such a way that all possible six target in-runs are monitored and a specially developed image comparison program immediately recognizes when a test organism has crossed the finish line. The runtime can now be read in automatically and saved for evaluation purposes.
Als Klein- und Kleinstlebewesen kann man z.B. Daphnien, Artemias, Hüpferlinge, Augentierchen, Plattwünner und Wimpertierchen verwenden.As a small and microorganism one can e.g. Use Daphnia, Artemias, Hupferlinge, eye-animals, flatworms and ciliates.
Dieses Wasserqualitäts-Test-Verfahren kann sehr flexibel an die jeweilige Situation angepasst werden, wobei man verschiedenste Testorganismen bis hin in den mikroskopischen Bereich einsetzen kann, wenn die Bewegungsreaktionen (Taxien) nicht nur durch Lichtreize, sondern auch durch Duftstoffe (Fleischköder), Stromimpulse oder Schallwellen ausgelöst werden kann.This water quality test method can be very flexibly adapted to the particular situation, where you can use a variety of test organisms down to the microscopic range, if the movement reactions (taxies) not only by light stimuli, but also by fragrances (meat baits), current pulses or Sound waves can be triggered.
In einer einfachen Variante kann der Tunnel direkt durch ein aquarienartiges Becken ersetzt werden, wobei ein Lichtbalken im Dunkeln die Testorganismen zeitkontrolliert anlockt. Dieser Lichtbalken wird nun zum Startbalken, nachdem das Licht gelöscht ist und der beleuchtete Gegenbalken die Organismen neuerlich anlockt, deren Wanderzeiten mittels Kamera und Zeitprogramm in Abhängigkeit der chemischen oder physikalischen Belastungen gut ermittelt werden kann.In a simple variant, the tunnel can be replaced directly with an aquarium-like basin, with a light bar in the dark attracting the test organisms in a time-controlled manner. This light bar now becomes the start bar after the light is extinguished and the illuminated counter bar lures the organisms again, whose traveling times can be determined well by means of camera and time program depending on the chemical or physical loads.
Im mikroskopischen Bereich kann dies - wie oben beschrieben- auch für lichtempfindliche Wimpertierchen und andere mikroskopisch erfassbare Ein- und Mehr-Zeller auf einer Objektträgermessvorrichtung durchgefuhrt werden. 16/05 2008 FR 17:07 [SE/EM NR 7171] @008In the microscopic range, this can also be carried out on a slide-type measuring device, as described above, for photosensitive ciliates and other microscopically detectable single and multiple cells. 16/05 2008 FR 17:07 [SE / EM NO 7171] @ 008
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT7872008A AT506827A1 (en) | 2008-05-16 | 2008-05-16 | Determining water quality, comprises measuring or evaluating migration speed of positive phototrophic water microbes such as daphnia, euglena, Triops, ciliate and Artemia salina-nauplii and -crayfish, which are attracted using light source |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT7872008A AT506827A1 (en) | 2008-05-16 | 2008-05-16 | Determining water quality, comprises measuring or evaluating migration speed of positive phototrophic water microbes such as daphnia, euglena, Triops, ciliate and Artemia salina-nauplii and -crayfish, which are attracted using light source |
Publications (1)
Publication Number | Publication Date |
---|---|
AT506827A1 true AT506827A1 (en) | 2009-12-15 |
Family
ID=41401984
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT7872008A AT506827A1 (en) | 2008-05-16 | 2008-05-16 | Determining water quality, comprises measuring or evaluating migration speed of positive phototrophic water microbes such as daphnia, euglena, Triops, ciliate and Artemia salina-nauplii and -crayfish, which are attracted using light source |
Country Status (1)
Country | Link |
---|---|
AT (1) | AT506827A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106526112A (en) * | 2016-10-25 | 2017-03-22 | 浙江工业大学 | Water toxicity detection method based on fish activity analysis |
CN113408334A (en) * | 2021-04-30 | 2021-09-17 | 华中农业大学 | Crayfish full-chain data acquisition and intelligent detection method and device |
CN114137160A (en) * | 2021-11-23 | 2022-03-04 | 重庆大学 | Migration experiment device and method for harmful gas in surrounding rock under tunnel excavation disturbance |
-
2008
- 2008-05-16 AT AT7872008A patent/AT506827A1/en not_active Application Discontinuation
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106526112A (en) * | 2016-10-25 | 2017-03-22 | 浙江工业大学 | Water toxicity detection method based on fish activity analysis |
CN113408334A (en) * | 2021-04-30 | 2021-09-17 | 华中农业大学 | Crayfish full-chain data acquisition and intelligent detection method and device |
CN114137160A (en) * | 2021-11-23 | 2022-03-04 | 重庆大学 | Migration experiment device and method for harmful gas in surrounding rock under tunnel excavation disturbance |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Michaels et al. | Cryptic zooplankton “swimmers” in upper ocean sediment traps | |
CN101512321B (en) | Method and apparatus for the detection of living phytoplankton cells in water | |
EP1970680A3 (en) | Optical sensor system for a device for treating fluids | |
CN106932553A (en) | A kind of online biosafety pre-alarming monitoring method of water quality | |
Cairns Jr | Ecological toxicity testing: scale, complexity, and relevance | |
Roskosch et al. | Bioirrigation by Chironomus plumosus: advective flow investigated by particle image velocimetry | |
US20210060558A1 (en) | Organism evaluation system and method of use | |
Díaz et al. | Tidal and wind-event variability and the distribution of two groups of Pseudo-nitzschia species in an upwelling-influenced Ría | |
AT506827A1 (en) | Determining water quality, comprises measuring or evaluating migration speed of positive phototrophic water microbes such as daphnia, euglena, Triops, ciliate and Artemia salina-nauplii and -crayfish, which are attracted using light source | |
DE69604575T2 (en) | METHOD AND DEVICE FOR GENERATING VERTICAL MEASURING PROFILES OF SEAWATER CHARACTERISTIC SIZES | |
Antipa | Use of commensal protozoa as biological indicators of water quality and pollution | |
Goncharuk et al. | Theoretical aspects of natural and drinking water biotesting | |
DE102014106891A1 (en) | Apparatus for testing water pipes for contamination | |
DE102009009165A1 (en) | Method and device for determining a path traveled by an endoscopy capsule in a patient | |
Palmer | Estimating the impact of laminar flow on the pattern and rate of decomposition in aquatic environments—Is there a better way of modeling decomposition? | |
Guterres et al. | Perna perna mussels network as pollution biosensors of oil spills and derivatives | |
AT516154A4 (en) | Method for determining the risk of corrosion of installations | |
DE102014012130B3 (en) | Detection method using recombinant living cells for the detection of xenobiotic substances and arrangement and test kit for performing the detection method | |
Vaishnavi et al. | Literature survey on smart water quality monitoring system | |
DE2728894C2 (en) | Device for registering the movement activity of animals on an opto-optoelectronic basis | |
Clark | Field studies in estuarine ecosystems: A review of approaches for assessing contaminant effects | |
Eggert et al. | Two complementary methods for studying amphibian terrestrial movements | |
Pinto et al. | Urban river health assessment and management | |
DE4234371C2 (en) | Device for monitoring drinking water or treated wastewater | |
Skrzypczak et al. | How to effectively collect zooplankton in illuminated cages for fish rearing? |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
REJ | Rejection |
Effective date: 20160515 |