AR126900A1 - Polipéptidos de coronavirus modificados genéticamente, polinucleótidos, vectores y células huésped, y composiciones relacionadas - Google Patents

Polipéptidos de coronavirus modificados genéticamente, polinucleótidos, vectores y células huésped, y composiciones relacionadas

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Publication number
AR126900A1
AR126900A1 ARP220102318A ARP220102318A AR126900A1 AR 126900 A1 AR126900 A1 AR 126900A1 AR P220102318 A ARP220102318 A AR P220102318A AR P220102318 A ARP220102318 A AR P220102318A AR 126900 A1 AR126900 A1 AR 126900A1
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Argentina
Prior art keywords
seq
polypeptide
rbd
amino acid
acid sequence
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ARP220102318A
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English (en)
Inventor
Ann M Arvin
Normand Blais
Davide Corti
Colin Havenar-Daughton
Matteo Samuele Pizzuto
Lionel Sacconnay
Gyorgy Snell
Original Assignee
Humabs Biomed Sa
Vir Biotechnology Inc
Glaxosmithkline Biologicals Sa
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Application filed by Humabs Biomed Sa, Vir Biotechnology Inc, Glaxosmithkline Biologicals Sa filed Critical Humabs Biomed Sa
Publication of AR126900A1 publication Critical patent/AR126900A1/es

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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/12Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/461Cellular immunotherapy characterised by the cell type used
    • A61K39/4611T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/463Cellular immunotherapy characterised by recombinant expression
    • A61K39/4634Antigenic peptides; polypeptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/46Cellular immunotherapy
    • A61K39/464Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/464838Viral antigens
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/14Antivirals for RNA viruses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1002Coronaviridae
    • C07K16/1003Severe acute respiratory syndrome coronavirus 2 [SARS‐CoV‐2 or Covid-19]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/62Medicinal preparations containing antigens or antibodies characterised by the link between antigen and carrier
    • A61K2039/627Medicinal preparations containing antigens or antibodies characterised by the link between antigen and carrier characterised by the linker
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/90Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/92Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/20011Coronaviridae
    • C12N2770/20022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/20011Coronaviridae
    • C12N2770/20034Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/36011Togaviridae
    • C12N2770/36111Alphavirus, e.g. Sindbis virus, VEE, EEE, WEE, Semliki
    • C12N2770/36141Use of virus, viral particle or viral elements as a vector
    • C12N2770/36143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Communicable Diseases (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Se proporcionan aquí polipéptidos de coronavirus de ingeniería, polinucleótidos que codifican los polipéptidos, vectores y células huésped que comprenden los polinucleótidos y/o expresan los polipéptidos, y composiciones relacionadas. Las formas de realización divulgadas incluyen, por ejemplo, ectodominios de espiga diseñados, dominios de unión a receptores monoméricos (RBD), RBD diseñados y proteínas de fusión que comprenden uno o más de los anteriores. Reivindicación 1: Una proteína de fusión que comprende un primer polipéptido de dominio de unión al receptor (RBD) de coronavirus unido a un segundo polipéptido RBD de coronavirus, en el que, opcionalmente, el primer y/o el segundo polipéptido RBD son de un betacoronavirus, y en el que, además, opcionalmente, el primer y el segundo polipéptido RBD son de diferentes betacoronavirus. Reivindicación 40: Un polipéptido aislado que comprende: (i) SEQ ID Nº 218; (ii) SEQ ID Nº 218 sin la SEQ ID Nº 706; (iii) SEQ ID Nº 712; (iv) SEQ ID Nº 712 sin SEQ ID Nº 706; (v) SEQ ID Nº 713; (vi) SEQ ID Nº 713 sin la SEQ ID Nº 706; (vii) SEQ ID Nº 714; (viii) SEQ ID Nº 714 sin la SEQ ID Nº 706; (ix) SEQ ID Nº 715; (x) SEQ ID Nº 715 sin la SEQ ID Nº 706; (xi) SEQ ID Nº 716; (xii) SEQ ID Nº 716 sin la SEQ ID Nº 706; (xiii) SEQ ID Nº 717; (xiv) SEQ ID Nº 717 sin la SEQ ID Nº 706; (xv) SEQ ID Nº 738; (xvi) SEQ ID Nº 739; (xvii) SEQ ID Nº 740; (xviii) SEQ ID Nº 741; (xix) SEQ ID Nº 742; o (xx) SEQ ID Nº 743. Reivindicación 42: Una proteína de fusión que comprende un polipéptido del dominio de unión al receptor (RBD) del coronavirus y uno o ambos de (i) y (ii), (i) una o más etiquetas peptídicas; (ii) uno o más polipéptidos de tallo-hélice, en los que, opcionalmente, el uno o más polipéptidos de tallo-hélice comprenden o consisten en la secuencia de aminoácidos PGSFKEELDKYFKNHTSDAASKAGP (SEQ ID Nº 701). Reivindicación 57: Un polipéptido aislado que comprende una estructura RBD1 - L1 - RBD2 - L2 - RBD3 - L3 - RBD4 - L4 - RBD5 en donde RBD1, RBD2, RBD3, RBD4 y RBD5 son cada uno un polipéptido RBD diferente seleccionado del grupo que consiste en: variante Beta del SARS-CoV-2; variante Omicron del SARS-CoV-2; variante Delta del SARS-CoV-2; PANG/GX; MP789; RaTG13; y RsSHC014, en donde RBD1 es el polipéptido RBD N-terminal de la proteína de fusión y/o RBD5 es el polipéptido RBD C-terminal de la proteína de fusión, y en donde L1, L2, L3 y L4 son cada uno un enlazador. Reivindicación 63: Un polipéptido aislado que comprende o consiste en la secuencia de aminoácidos de cualquiera de las SEQ ID Nº 701, 4 - 19, 46 y 57 - 59. Reivindicación 64: Un polipéptido aislado que comprende o consiste en la secuencia de aminoácidos de SEQ ID Nº 701, la secuencia de aminoácidos de SEQ ID Nº 14, la secuencia de aminoácidos de SEQ ID Nº 15, la secuencia de aminoácidos de SEQ ID Nº 16, o la secuencia de aminoácidos de SEQ ID Nº 17. Reivindicación 65: Un polinucleótido aislado que codifica la proteína de fusión de acuerdo con cualquiera de las reivindicaciones 1 - 39 y 42 - 56, o el polipéptido de acuerdo con cualquiera de las reivindicaciones 40, 41 y 57 - 64. Reivindicación 72: Un método para tratar, o para inducir una respuesta inmunitaria contra una infección por coronavirus en un sujeto, el método comprende administrar al sujeto una cantidad eficaz de: la proteína de fusión de acuerdo con cualquiera de las reivindicaciones 1 - 39 y 42 - 56; el polipéptido de acuerdo con cualquiera de las reivindicaciones 40, 41 y 57 - 64; el polinucleótido de acuerdo con cualquiera de las reivindicaciones 65 - 67; el vector de acuerdo con la reivindicación 68; la célula huésped de acuerdo con la reivindicación 69; o la composición de acuerdo con la reivindicación 70 o 71, en donde, opcionalmente, la infección por coronavirus comprende una infección por sarbecovirus.
ARP220102318A 2021-08-27 2022-08-26 Polipéptidos de coronavirus modificados genéticamente, polinucleótidos, vectores y células huésped, y composiciones relacionadas AR126900A1 (es)

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US202163238078P 2021-08-27 2021-08-27

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AR (1) AR126900A1 (es)
TW (1) TW202315895A (es)
WO (1) WO2023028603A2 (es)

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Publication number Priority date Publication date Assignee Title
IL299630A (en) 2020-07-06 2023-03-01 Flagship Pioneering Innovations Vi Llc Antigen binding molecules targeting SARS Cov2
KR20230058434A (ko) 2020-08-26 2023-05-03 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 SARS-CoV-2를 표적으로 하는 항원 결합 분자

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