AR124835A1 - Métodos y composiciones para la crioconservación de células inmunitarias - Google Patents
Métodos y composiciones para la crioconservación de células inmunitariasInfo
- Publication number
- AR124835A1 AR124835A1 ARP220100250A ARP220100250A AR124835A1 AR 124835 A1 AR124835 A1 AR 124835A1 AR P220100250 A ARP220100250 A AR P220100250A AR P220100250 A ARP220100250 A AR P220100250A AR 124835 A1 AR124835 A1 AR 124835A1
- Authority
- AR
- Argentina
- Prior art keywords
- cells
- cryopreservation medium
- cryopreservation
- cryoprotectant
- hsa
- Prior art date
Links
- 210000002865 immune cell Anatomy 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 238000005138 cryopreservation Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title 1
- 239000012595 freezing medium Substances 0.000 abstract 9
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 8
- 102000008100 Human Serum Albumin Human genes 0.000 abstract 7
- 108091006905 Human Serum Albumin Proteins 0.000 abstract 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 6
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 abstract 5
- 210000004027 cell Anatomy 0.000 abstract 5
- 210000000822 natural killer cell Anatomy 0.000 abstract 5
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 abstract 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 abstract 4
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 abstract 4
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 abstract 4
- 239000002577 cryoprotective agent Substances 0.000 abstract 4
- 102000009027 Albumins Human genes 0.000 abstract 3
- 108010088751 Albumins Proteins 0.000 abstract 3
- 150000002016 disaccharides Chemical class 0.000 abstract 3
- 230000001698 pyrogenic effect Effects 0.000 abstract 3
- 239000011780 sodium chloride Substances 0.000 abstract 3
- AEQDJSLRWYMAQI-UHFFFAOYSA-N 2,3,9,10-tetramethoxy-6,8,13,13a-tetrahydro-5H-isoquinolino[2,1-b]isoquinoline Chemical compound C1CN2CC(C(=C(OC)C=C3)OC)=C3CC2C2=C1C=C(OC)C(OC)=C2 AEQDJSLRWYMAQI-UHFFFAOYSA-N 0.000 abstract 2
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 abstract 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 2
- 238000002659 cell therapy Methods 0.000 abstract 2
- 210000004700 fetal blood Anatomy 0.000 abstract 2
- 229910001629 magnesium chloride Inorganic materials 0.000 abstract 2
- FVVLHONNBARESJ-NTOWJWGLSA-H magnesium;potassium;trisodium;(2r,3s,4r,5r)-2,3,4,5,6-pentahydroxyhexanoate;acetate;tetrachloride;nonahydrate Chemical compound O.O.O.O.O.O.O.O.O.[Na+].[Na+].[Na+].[Mg+2].[Cl-].[Cl-].[Cl-].[Cl-].[K+].CC([O-])=O.OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O FVVLHONNBARESJ-NTOWJWGLSA-H 0.000 abstract 2
- 239000002609 medium Substances 0.000 abstract 2
- 235000017281 sodium acetate Nutrition 0.000 abstract 2
- 229940087562 sodium acetate trihydrate Drugs 0.000 abstract 2
- 239000000176 sodium gluconate Substances 0.000 abstract 2
- 235000012207 sodium gluconate Nutrition 0.000 abstract 2
- 229940005574 sodium gluconate Drugs 0.000 abstract 2
- 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 abstract 1
- 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 abstract 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 235000011147 magnesium chloride Nutrition 0.000 abstract 1
- LQBKAYJFACGUCC-UHFFFAOYSA-N n-(5-cyclopropyl-1h-pyrazol-3-yl)-2-(4-thiophen-2-ylphenyl)acetamide Chemical compound C1=C(C2CC2)NN=C1NC(=O)CC(C=C1)=CC=C1C1=CC=CS1 LQBKAYJFACGUCC-UHFFFAOYSA-N 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 239000011591 potassium Substances 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- 230000001177 retroviral effect Effects 0.000 abstract 1
- 235000002639 sodium chloride Nutrition 0.000 abstract 1
- 239000012808 vapor phase Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Biophysics (AREA)
- Physiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
Abstract
La presente descripción proporciona, entre otras cosas, un medio de crioconservación que comprende un criprotector, una albúmina, un disacárido y una solución cristaloide isotónica y no pirógena. La descripción también proporciona, entre otras cosas, un medio de crioconservación para crioconservar células inmunitarias, tal medio comprende: albúmina de suero humano (HSA), cloruro de sodio, gluconato de sodio, trihidrato de acetato de sodio, cloruro de sodio, cloruro de potasio, cloruro de magnesio, sulfóxido de dimetilo (DMSO) y una trehalosa. La presente descripción también proporciona un método para crioconservar células inmunitarias, transportar y posteriormente administrar dichas células inmunitarias a un paciente que las necesite. Reivindicación 1: Un medio de crioconservación que comprende un crioprotector, una albúmina, un disacárido y una solución cristaloide isotónica y no pirógena. Reivindicación 13: Un medio de crioconservación que comprende: albúmina de suero humano (HSA), cloruro de sodio, gluconato de sodio, trihidrato de acetato de sodio, cloruro de potasio, cloruro de magnesio, sulfóxido de dimetilo (DMSO) y una trehalosa. Reivindicación 17: Un medio de crioconservación adecuado para células inmunitarias, tal medio comprende: PLASMA-LYTE A, albúmina de suero humano (HSA), trehalosa y un crioprotector. Reivindicación 31: Un método de crioconservación de linfocitos citolíticos naturales (NK), tal método comprende: (a) poner en contacto las células NK con un medio de crioconservación que comprende un crioprotector, una albúmina, un disacárido y una solución cristaloide isotónica y no pirógena; (b) enfriar las células a una temperatura de -80ºC; y (c) almacenar las células en fase de vapor de nitrógeno líquido, crioconservando así las células NK. Reivindicación 39: Un producto de terapia celular para administrar a un sujeto que lo necesite que comprende: (a) una población de células NK modificadas que comprende células de sangre del cordón umbilical transducidas con un vector retroviral que expresa receptor de antígeno quimérico (CAR) anti-CD19, IL-15 e iCaspasa9; y (b) un medio de crioconservación de acuerdo con cualquiera de las reivindicaciones 1 - 30. Reivindicación 40: Una población de células NK del receptor de antígeno quimérico CD19 (CAR) en un medio de crioconservación de cualquiera de las reivindicaciones 1 - 29. Reivindicación 43: Un producto de terapia celular que comprende una población de células CAR-NK que comprende células NK de sangre del cordón umbilical modificadas genéticamente para expresar un CD-19 CAR, una iCaspasa y una IL-15 formuladas en un medio de crioconservación que comprende PLASMA-LYTE A, trehalosa, CS10 y HSA.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163147739P | 2021-02-09 | 2021-02-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR124835A1 true AR124835A1 (es) | 2023-05-10 |
Family
ID=80446155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220100250A AR124835A1 (es) | 2021-02-09 | 2022-02-08 | Métodos y composiciones para la crioconservación de células inmunitarias |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20240306632A1 (es) |
| AR (1) | AR124835A1 (es) |
| TW (1) | TW202245597A (es) |
| WO (1) | WO2022173736A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777686A (zh) * | 2022-09-06 | 2023-03-14 | 北京唐颐惠康生物医学技术有限公司 | 一种细胞冻存液及其制备方法和应用 |
| CN118633593B (zh) * | 2024-08-09 | 2024-11-05 | 北京百奥益康医药科技有限公司 | 一种用于单细胞测序的组织冻存液及其制备方法和应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200077613A (ko) * | 2010-07-13 | 2020-06-30 | 안트로제네시스 코포레이션 | 천연 킬러 세포의 생성 방법 |
| WO2016063208A1 (en) * | 2014-10-20 | 2016-04-28 | Stempeutics Research Pvt. Ltd. | A cryopreservation composition and methods thereof |
-
2022
- 2022-02-08 AR ARP220100250A patent/AR124835A1/es unknown
- 2022-02-08 WO PCT/US2022/015626 patent/WO2022173736A1/en not_active Application Discontinuation
- 2022-02-08 TW TW111104550A patent/TW202245597A/zh unknown
- 2022-02-09 US US18/274,988 patent/US20240306632A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| TW202245597A (zh) | 2022-12-01 |
| WO2022173736A1 (en) | 2022-08-18 |
| US20240306632A1 (en) | 2024-09-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Chaytor et al. | Inhibiting ice recrystallization and optimization of cell viability after cryopreservation | |
| AR124835A1 (es) | Métodos y composiciones para la crioconservación de células inmunitarias | |
| Huang et al. | Predehydration and ice seeding in the presence of trehalose enable cell cryopreservation | |
| Oldenhof et al. | Osmotic stress and membrane phase changes during freezing of stallion sperm: mode of action of cryoprotective agents | |
| US6596531B2 (en) | Two stage method for thawing cryopreserved cells | |
| JP6438887B2 (ja) | 凍結防止剤、凍結防止および凍結保存組成物、その使用ならびに凍結保存方法 | |
| Motta et al. | Evaluation of intracellular and extracellular trehalose as a cryoprotectant of stem cells obtained from umbilical cord blood | |
| Courbiere et al. | Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols | |
| Natan et al. | Freeze-drying of mononuclear cells derived from umbilical cord blood followed by colony formation | |
| Yao et al. | Cryopreservation of NK and T cells without DMSO for adoptive cell-based immunotherapy | |
| JP7038369B2 (ja) | ウシ生殖細胞の凍結保存用組成物及び凍結保存方法 | |
| Yang et al. | A hemocompatible cryoprotectant inspired by freezing-tolerant plants | |
| Freitas-Ribeiro et al. | Long-term and short-term preservation strategies for tissue engineering and regenerative medicine products: state of the art and emerging trends | |
| William et al. | Engineered compounds to control ice nucleation and recrystallization | |
| Hawkins et al. | Biopreservation Best Practices for regenerative medicine GMP manufacturing & focus on optimized biopreservation media | |
| EP3064211B1 (en) | Sperm-motility enhancer | |
| Wang et al. | FTIR analysis of molecular changes associated with warming injury in cryopreserved leukocytes | |
| WO2019100175A1 (es) | Composición y método para el cultivo, expansión, preservación y/o pretratamiento de células | |
| Berz et al. | Cryopreservation of hematopoietic and non-hematopoietic stem cells–a review for the clinician | |
| Murray et al. | Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities | |
| KR20230145095A (ko) | 포유동물 세포를 동결 및 해동하기 위한 방법 및 조성물 | |
| Fakhrildin et al. | Effect of two types and two concentrations of cryoprotectants on ovine oocytes morphology and viability post-vitrification | |
| JP2005270006A (ja) | 精子凍結保存剤 | |
| Keros et al. | Cryopreservation of mammalian oocytes | |
| FR3038325A1 (fr) | Procede de cryoconservation de cellules a visee therapeutique |