AR110954A1 - METHODS OF GLUCOSILATION OF GLYCOSILTRANSPHERASES AND ESTEVIOL GLYCOSIDE MANIPULATED BY ENGINEERING - Google Patents

METHODS OF GLUCOSILATION OF GLYCOSILTRANSPHERASES AND ESTEVIOL GLYCOSIDE MANIPULATED BY ENGINEERING

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Publication number
AR110954A1
AR110954A1 ARP180100242A ARP180100242A AR110954A1 AR 110954 A1 AR110954 A1 AR 110954A1 AR P180100242 A ARP180100242 A AR P180100242A AR P180100242 A ARP180100242 A AR P180100242A AR 110954 A1 AR110954 A1 AR 110954A1
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AR
Argentina
Prior art keywords
enzymes
methods
manipulated
engineering
rebaudioside
Prior art date
Application number
ARP180100242A
Other languages
Spanish (es)
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of AR110954A1 publication Critical patent/AR110954A1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente proporciona enzimas de glicosiltransferasa manipulada por ingeniería (GT), polipéptidos que tienen actividad de GT y polinucleótidos que codifican estas enzimas, así como vectores y células huésped que comprenden estos polinucleótidos y polipéptidos. La presente proporciona enzimas de sacarosa sintasa manipulada por ingeniería (SuS), polipéptidos que tienen actividad de SuS y polinucleótidos que codifican estas enzimas, así como vectores y células huésped que comprenden estos polinucleótidos y polipéptidos. La presente también proporciona composiciones que comprenden las enzimas GT y métodos de uso de las enzimas GT manipuladas por ingeniería para preparar productos con ligaciones de b-glucosa. La presente también proporciona composiciones y métodos para la producción de rebaudiósidos (por ejemplo, rebaudiósido M, rebaudiósido A, rebaudiósido I y rebaudiósido D). La presente también proporciona composiciones que comprenden las enzimas SuS y métodos de uso de ellas. También se proporcionan métodos para producir enzimas GT y SuS.This provides engineering manipulated glycosyltransferase (GT) enzymes, polypeptides having GT activity and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. This provides engineering manipulated sucrose synthase enzymes (SuS), polypeptides having SuS activity and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. This also provides compositions comprising GT enzymes and methods of using engineered GT enzymes to prepare products with b-glucose linkages. The present also provides compositions and methods for the production of rebaudiosides (for example, rebaudioside M, rebaudioside A, rebaudioside I and rebaudioside D). The present also provides compositions comprising the SuS enzymes and methods of using them. Methods for producing GT and SuS enzymes are also provided.

ARP180100242A 2017-02-03 2018-02-01 METHODS OF GLUCOSILATION OF GLYCOSILTRANSPHERASES AND ESTEVIOL GLYCOSIDE MANIPULATED BY ENGINEERING AR110954A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762454417P 2017-02-03 2017-02-03

Publications (1)

Publication Number Publication Date
AR110954A1 true AR110954A1 (en) 2019-05-22

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ARP180100242A AR110954A1 (en) 2017-02-03 2018-02-01 METHODS OF GLUCOSILATION OF GLYCOSILTRANSPHERASES AND ESTEVIOL GLYCOSIDE MANIPULATED BY ENGINEERING

Country Status (1)

Country Link
AR (1) AR110954A1 (en)

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