AR105760A1 - METHODS AND CONSTRUCTS FOR THE SPECIFIC MODIFICATION OF NUCLEIC ACIDS IN PLANTS - Google Patents

METHODS AND CONSTRUCTS FOR THE SPECIFIC MODIFICATION OF NUCLEIC ACIDS IN PLANTS

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Publication number
AR105760A1
AR105760A1 ARP160101461A ARP160101461A AR105760A1 AR 105760 A1 AR105760 A1 AR 105760A1 AR P160101461 A ARP160101461 A AR P160101461A AR P160101461 A ARP160101461 A AR P160101461A AR 105760 A1 AR105760 A1 AR 105760A1
Authority
AR
Argentina
Prior art keywords
constructs
plants
nucleic acids
methods
plant
Prior art date
Application number
ARP160101461A
Other languages
Spanish (es)
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of AR105760A1 publication Critical patent/AR105760A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente se refiere a procedimientos y constructos para la modificación selectiva de una región de ácidos nucleicos blanco en una estructura de planta blanco. En especial, la presente se refiere a procedimientos y constructos para la obtención directa de plantas o de material de planta, que comprenden una edición de un ácido nucleico, introducida anteriormente de manera selectiva en una célula meristemática. Los constructos pueden ser introducidos de manera transitoria y/o estable. A tal efecto se divulgan estrategias para la introducción, optimizadas en función de las plantas. Además, la presente comprende un procedimiento para llevar a cabo un ensayo de selección sistemática in vitro, a efectos de verificar de antemano in vitro gARNs candidato adecuados en cuanto su eficiencia. Planta.This refers to procedures and constructs for the selective modification of a region of white nucleic acids in a white plant structure. In particular, this refers to procedures and constructs for the direct obtaining of plants or plant material, which comprise an edition of a nucleic acid, previously introduced selectively into a meristematic cell. The constructs can be introduced transiently and / or stably. To this end, strategies for the introduction are disclosed, optimized according to the plants. In addition, the present invention comprises a procedure for carrying out a systematic in vitro screening test, in order to verify in advance in vitro suitable candidate gRNAs for their efficiency. Plant.

ARP160101461A 2015-05-19 2016-05-19 METHODS AND CONSTRUCTS FOR THE SPECIFIC MODIFICATION OF NUCLEIC ACIDS IN PLANTS AR105760A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102015006335.9A DE102015006335A1 (en) 2015-05-19 2015-05-19 Methods and constructs for targeted nucleic acid editing in plants

Publications (1)

Publication Number Publication Date
AR105760A1 true AR105760A1 (en) 2017-11-08

Family

ID=57231505

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP160101461A AR105760A1 (en) 2015-05-19 2016-05-19 METHODS AND CONSTRUCTS FOR THE SPECIFIC MODIFICATION OF NUCLEIC ACIDS IN PLANTS
ARP160101462A AR104702A1 (en) 2015-05-19 2016-05-19 METHODS FOR THE TRANSFORMATION OF IN PLANT PLANTS, AND MANUFACTURING PROCESSES OF A TRANSGENIC PLANT

Family Applications After (1)

Application Number Title Priority Date Filing Date
ARP160101462A AR104702A1 (en) 2015-05-19 2016-05-19 METHODS FOR THE TRANSFORMATION OF IN PLANT PLANTS, AND MANUFACTURING PROCESSES OF A TRANSGENIC PLANT

Country Status (2)

Country Link
AR (2) AR105760A1 (en)
DE (1) DE102015006335A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2016002306A (en) 2013-08-22 2016-07-08 Du Pont A soybean u6 polymerase iii promoter and methods of use.
BR112017017260A2 (en) 2015-03-27 2018-04-17 Du Pont dna constructs, vector, cell, plants, seed, rna expression method, and target site modification method
WO2017195906A1 (en) * 2016-05-13 2017-11-16 株式会社カネカ Plant genome editing method
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
EP3456181A4 (en) 2016-05-13 2019-12-25 Kaneka Corporation Method for creating transformed plant
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
CN108588243A (en) * 2018-08-06 2018-09-28 中国计量大学 Quickly differentiate specific primer, multiple PCR detection kit and its detection method of adventive Pomacea canaliculata
CN113166744A (en) 2018-12-14 2021-07-23 先锋国际良种公司 Novel CRISPR-CAS system for genome editing

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
MX2016002306A (en) 2013-08-22 2016-07-08 Du Pont A soybean u6 polymerase iii promoter and methods of use.

Also Published As

Publication number Publication date
DE102015006335A1 (en) 2016-11-24
AR104702A1 (en) 2017-08-09

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