AR093980A1 - Genes de precision que tienen como objetivo un locus particular en el maiz - Google Patents
Genes de precision que tienen como objetivo un locus particular en el maizInfo
- Publication number
- AR093980A1 AR093980A1 ARP130104697A ARP130104697A AR093980A1 AR 093980 A1 AR093980 A1 AR 093980A1 AR P130104697 A ARP130104697 A AR P130104697A AR P130104697 A ARP130104697 A AR P130104697A AR 093980 A1 AR093980 A1 AR 093980A1
- Authority
- AR
- Argentina
- Prior art keywords
- corn
- tolerance
- resistance
- genes
- objective
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07047—Streptomycin 3''-adenylyltransferase (2.7.7.47)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Nutrition Science (AREA)
- Insects & Arthropods (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pest Control & Pesticides (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
La presente reivindica los métodos para la integración estable del ADN exógeno en un locus específico E32 en el genoma del maíz mediante el uso de nucleasas de dedos de cinc. Se reivindican las plantas de maíz y las partes de las plantas que fueron transformadas por los métodos. La presente es útil para la creación de rasgos deseables tales como la resistencia a los herbicidas, la tolerancia a los herbicidas, la resistencia a los insectos, la tolerancia a los insectos, resistencia a las enfermedades, tolerancia a las enfermedades, la tolerancia al estrés y resistencia al estrés en el maíz. El locus E32 representa un sitio superior para la inserción de los genes extraños porque los fenotipos nativos agronómicos no están interrumpidos.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261736856P | 2012-12-13 | 2012-12-13 | |
US201361820231P | 2013-05-07 | 2013-05-07 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR093980A1 true AR093980A1 (es) | 2015-07-01 |
Family
ID=49883309
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP130104697A AR093980A1 (es) | 2012-12-13 | 2013-12-13 | Genes de precision que tienen como objetivo un locus particular en el maiz |
Country Status (14)
Country | Link |
---|---|
US (2) | US20140173783A1 (es) |
EP (1) | EP2931901A1 (es) |
JP (1) | JP2016500268A (es) |
KR (1) | KR20150096696A (es) |
CN (1) | CN105378088A (es) |
AR (1) | AR093980A1 (es) |
AU (1) | AU2013359091A1 (es) |
BR (1) | BR102013032200A2 (es) |
CA (1) | CA2895117A1 (es) |
IL (1) | IL239263A0 (es) |
MX (1) | MX2015007574A (es) |
RU (1) | RU2015128052A (es) |
WO (1) | WO2014093768A1 (es) |
ZA (1) | ZA201504199B (es) |
Families Citing this family (33)
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US10030245B2 (en) | 2011-03-23 | 2018-07-24 | E I Du Pont De Nemours And Company | Methods for producing a complex transgenic trait locus |
AU2012333134B2 (en) | 2011-07-22 | 2017-05-25 | John Paul Guilinger | Evaluation and improvement of nuclease cleavage specificity |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
BR112016003591A8 (pt) | 2013-08-22 | 2018-01-30 | Du Pont | promotor u6 de polimerase iii de soja e métodos de uso |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
WO2015070083A1 (en) | 2013-11-07 | 2015-05-14 | Editas Medicine,Inc. | CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
WO2016007347A1 (en) | 2014-07-11 | 2016-01-14 | E. I. Du Pont De Nemours And Company | Compositions and methods for producing plants resistant to glyphosate herbicide |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3191595B1 (en) * | 2014-09-12 | 2019-12-25 | E. I. du Pont de Nemours and Company | Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use |
EP3274460A1 (en) | 2015-03-27 | 2018-01-31 | E. I. du Pont de Nemours and Company | Soybean u6 small nuclear rna gene promoters and their use in constitutive expression of small rna genes in plants |
WO2017070632A2 (en) | 2015-10-23 | 2017-04-27 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
WO2017123609A1 (en) * | 2016-01-12 | 2017-07-20 | The Regents Of The University Of California | Compositions and methods for enhanced genome editing |
CA3032699A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
KR102687373B1 (ko) | 2017-03-23 | 2024-07-23 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
KR20200124702A (ko) | 2018-02-23 | 2020-11-03 | 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 | 신규한 cas9 오르소로그 |
WO2020123887A2 (en) | 2018-12-14 | 2020-06-18 | Pioneer Hi-Bred International, Inc. | Novel crispr-cas systems for genome editing |
WO2020157164A1 (en) | 2019-01-30 | 2020-08-06 | Enobraq | Modified plant with improved rubisco activity |
WO2020191249A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
MX2022014008A (es) | 2020-05-08 | 2023-02-09 | Broad Inst Inc | Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo. |
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-
2013
- 2013-12-13 RU RU2015128052A patent/RU2015128052A/ru not_active Application Discontinuation
- 2013-12-13 AR ARP130104697A patent/AR093980A1/es unknown
- 2013-12-13 CA CA2895117A patent/CA2895117A1/en not_active Abandoned
- 2013-12-13 EP EP13814382.1A patent/EP2931901A1/en not_active Withdrawn
- 2013-12-13 MX MX2015007574A patent/MX2015007574A/es unknown
- 2013-12-13 AU AU2013359091A patent/AU2013359091A1/en not_active Abandoned
- 2013-12-13 WO PCT/US2013/074916 patent/WO2014093768A1/en active Application Filing
- 2013-12-13 BR BR102013032200A patent/BR102013032200A2/pt not_active IP Right Cessation
- 2013-12-13 JP JP2015547974A patent/JP2016500268A/ja active Pending
- 2013-12-13 US US14/105,563 patent/US20140173783A1/en not_active Abandoned
- 2013-12-13 CN CN201380065591.4A patent/CN105378088A/zh active Pending
- 2013-12-13 KR KR1020157018279A patent/KR20150096696A/ko not_active Application Discontinuation
-
2015
- 2015-06-07 IL IL239263A patent/IL239263A0/en unknown
- 2015-06-10 ZA ZA2015/04199A patent/ZA201504199B/en unknown
-
2016
- 2016-10-31 US US15/338,888 patent/US10415046B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
US20140173783A1 (en) | 2014-06-19 |
US20170044558A1 (en) | 2017-02-16 |
CA2895117A1 (en) | 2014-06-19 |
RU2015128052A (ru) | 2017-01-19 |
MX2015007574A (es) | 2015-10-22 |
ZA201504199B (en) | 2016-11-30 |
IL239263A0 (en) | 2015-07-30 |
EP2931901A1 (en) | 2015-10-21 |
KR20150096696A (ko) | 2015-08-25 |
AU2013359091A1 (en) | 2015-07-02 |
BR102013032200A2 (pt) | 2015-11-24 |
CN105378088A (zh) | 2016-03-02 |
US10415046B2 (en) | 2019-09-17 |
WO2014093768A1 (en) | 2014-06-19 |
JP2016500268A (ja) | 2016-01-12 |
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