AR091033A1 - Metodo para producir 11-des-o-metiltomaimicina recombinante - Google Patents

Metodo para producir 11-des-o-metiltomaimicina recombinante

Info

Publication number
AR091033A1
AR091033A1 ARP130101648A AR091033A1 AR 091033 A1 AR091033 A1 AR 091033A1 AR P130101648 A ARP130101648 A AR P130101648A AR 091033 A1 AR091033 A1 AR 091033A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
seq
variant
fragment
functionally active
Prior art date
Application number
Other languages
English (en)
Original Assignee
Sanofi Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Sa filed Critical Sanofi Sa
Publication of AR091033A1 publication Critical patent/AR091033A1/es

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D519/00—Heterocyclic compounds containing more than one system of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring system not provided for in groups C07D453/00 or C07D455/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Reivindicación 1: Un ácido nucleico que comprende al menos un ácido nucleico seleccionado de: (a) un ácido nucleico que comprende al menos una de las 1 a 19 Fases de Lectura Abierta (ORF) como se comprende en la SEC ID Nº 2 que codifica las proteínas de las SEC ID Nº 4 a 22 o una variante o un fragmento del mismo en el que la variante o el fragmento codifica una variante o un fragmento funcionalmente activo de una proteína de las SEC ID Nº 4 a 22, (b) un ácido nucleico que codifica al menos una de las proteínas de las SEC ID Nº 4 a 22 o una variante o un fragmento funcionalmente activo del mismo, (c) un ácido nucleico que codifica una proteína que tiene una identidad de secuencia de aminoácidos de al menos 70%, 80%, 90%, 95% ó 97% con una proteína o un fragmento de la misma codificada por el ácido nucleico de (a) ó (b), (d) un ácido nucleico que se hibrida en condiciones rigurosas con un ácido nucleico de (a) a (c), (e) un ácido nucleico que es complementario a un ácido nucleico de (a) a (d). Reivindicación 3: Un vector de expresión que comprende un ácido nucleico de la reivindicación 1 ó 2. Reivindicación 4: Una célula que comprende el ácido nucleico de acuerdo con la reivindicación 1 ó 2 o que se transforma con el vector de expresión de acuerdo con la reivindicación 3, preferentemente la célula es la especie de Streptomyces FH6421. Reivindicación 5: Un método en el que una célula contiene un grupo génico biosintético de tomaimicina o una variante o un fragmento funcionalmente activo del mismo. Reivindicación 9: El método de acuerdo con la reivindicación 5 en el que la célula contiene al menos una ORF comprendida en la SEC ID Nº 1, SEC ID Nº 2 ó SEC ID Nº 3 o variantes o fragmentos funcionalmente activos de la misma y que puede producir 11-des-O-metiltomaimicina.
ARP130101648 2012-05-14 2013-05-13 Metodo para producir 11-des-o-metiltomaimicina recombinante AR091033A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP12305529 2012-05-14

Publications (1)

Publication Number Publication Date
AR091033A1 true AR091033A1 (es) 2014-12-30

Family

ID=48430770

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130101648 AR091033A1 (es) 2012-05-14 2013-05-13 Metodo para producir 11-des-o-metiltomaimicina recombinante

Country Status (5)

Country Link
US (2) US10550397B2 (es)
EP (1) EP2849753B1 (es)
AR (1) AR091033A1 (es)
TW (1) TW201406951A (es)
WO (1) WO2013171157A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10201809668TA (en) 2014-09-12 2018-11-29 Genentech Inc Anti-her2 antibodies and immunoconjugates
MA43345A (fr) 2015-10-02 2018-08-08 Hoffmann La Roche Conjugués anticorps-médicaments de pyrrolobenzodiazépine et méthodes d'utilisation
US10975403B2 (en) 2017-08-09 2021-04-13 Conagen Inc. Biosynthesis of eriodictyol from engineered microbes
WO2020077367A1 (en) * 2018-10-12 2020-04-16 Conagen Inc. Biosynthesis of homoeriodictyol

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1516743A (fr) 1966-04-01 1968-02-05 Rhone Poulenc Sa Nouvel antibiotique et son procédé de préparation par culture de streptomyces croceus
ZA698792B (en) * 1968-12-30 1971-03-31 Fujisawa Pharmaceutical Co Novel antibiotic and derivatives and production thereof
US7291490B2 (en) * 2001-06-11 2007-11-06 Ecopia Biosciences Inc. Nucleic acid fragment encoding an NRPS for the biosynthesis of anthramycin

Also Published As

Publication number Publication date
US20150111298A1 (en) 2015-04-23
US10550397B2 (en) 2020-02-04
EP2849753A1 (en) 2015-03-25
WO2013171157A1 (en) 2013-11-21
US20200216852A1 (en) 2020-07-09
EP2849753B1 (en) 2022-08-10
TW201406951A (zh) 2014-02-16

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