AR088696A1 - Endonucleasa para edicion de genoma - Google Patents

Endonucleasa para edicion de genoma

Info

Publication number
AR088696A1
AR088696A1 ARP120104197A ARP120104197A AR088696A1 AR 088696 A1 AR088696 A1 AR 088696A1 AR P120104197 A ARP120104197 A AR P120104197A AR P120104197 A ARP120104197 A AR P120104197A AR 088696 A1 AR088696 A1 AR 088696A1
Authority
AR
Argentina
Prior art keywords
endonuclease
genoma
edition
domain
yig
Prior art date
Application number
ARP120104197A
Other languages
English (en)
Inventor
Rohan Edgell David
Peter Kleinstiver Benjamin
Original Assignee
Univ Western Ontario
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Western Ontario filed Critical Univ Western Ontario
Publication of AR088696A1 publication Critical patent/AR088696A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporciona una endonucleasa quimérica que comprende el dominio de nucleasa GIY-YIG que está ligado a un dominio dirigido a ADN por un dominio de ligación. La endonucleasa es de utilidad en la edición de genes. Célula. Kit. Sustrato de ácido nucléico.
ARP120104197A 2011-11-07 2012-11-08 Endonucleasa para edicion de genoma AR088696A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161628810P 2011-11-07 2011-11-07
US201261701545P 2012-09-14 2012-09-14

Publications (1)

Publication Number Publication Date
AR088696A1 true AR088696A1 (es) 2014-06-25

Family

ID=48290707

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP120104197A AR088696A1 (es) 2011-11-07 2012-11-08 Endonucleasa para edicion de genoma

Country Status (4)

Country Link
US (1) US20130210151A1 (es)
EP (1) EP2776560A2 (es)
AR (1) AR088696A1 (es)
WO (1) WO2013068845A2 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2758537A4 (en) * 2011-09-23 2015-08-12 Univ Iowa State Res Found ARCHITECTURE OF TAL NUCLEASE MONOMER OR ZINC FINGER NUCLEASE FOR DNA MODIFICATION
WO2014121222A1 (en) * 2013-02-01 2014-08-07 The University Of Western Ontario Endonuclease for genome editing
EP2951295B1 (en) * 2013-02-01 2018-08-01 Cellectis Tevi chimeric endonucleases and their preferential cleavage sites
CA2913872C (en) * 2013-05-31 2022-01-18 Cellectis A laglidadg homing endonuclease cleaving the t-cell receptor alpha gene and uses thereof
JP6488283B2 (ja) * 2013-05-31 2019-03-20 セレクティスＣｅｌｌｅｃｔｉｓ Ｃ−ｃケモカイン受容体５型（ｃｃｒ５）遺伝子を開裂するｌａｇｌｉｄａｄｇホーミングエンドヌクレアーゼおよびその用途
MX2016002118A (es) 2013-08-22 2016-06-28 Du Pont Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso.
KR102251168B1 (ko) * 2013-10-25 2021-05-13 셀렉티스 고 반복 모티프를 포함하는 dna 서열에 대한 효율적이고 특이적인 표적화를 위한 희소-절단 엔도뉴클레아제의 설계
AU2015288157A1 (en) 2014-07-11 2017-01-19 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
CN108064129A (zh) 2014-09-12 2018-05-22 纳幕尔杜邦公司 玉米和大豆中复合性状基因座的位点特异性整合位点的产生和使用方法
EP3553178A1 (en) 2015-03-27 2019-10-16 E. I. du Pont de Nemours and Company Soybean u6 small nuclear rna gene promoters and their use in constitutive expression of small rna genes in plants
US20200332306A1 (en) * 2017-10-09 2020-10-22 Pioneer Hi-Bred International, Inc. Type i-e crispr-cas systems for eukaryotic genome editing
US20210371847A1 (en) * 2018-06-27 2021-12-02 Altius Institute For Biomedical Sciences Gapped And Tunable Repeat Units For Use In Genome Editing And Gene Regulation Compositions
US20220010322A1 (en) * 2018-12-04 2022-01-13 Syngenta Crop Protection Ag Gene silencing via genome editing
WO2020123887A2 (en) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
MX2021013400A (es) * 2019-05-03 2022-04-20 Specific Biologics Inc Endonucleasa de escision dual encapsulada en lípido para edición de gen y adn.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8956828B2 (en) * 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
CN102762726A (zh) * 2009-11-27 2012-10-31 巴斯夫植物科学有限公司 嵌合内切核酸酶及其用途
US20140115726A1 (en) 2011-04-05 2014-04-24 Cellectis New tale-protein scaffolds and uses thereof
EP2758537A4 (en) * 2011-09-23 2015-08-12 Univ Iowa State Res Found ARCHITECTURE OF TAL NUCLEASE MONOMER OR ZINC FINGER NUCLEASE FOR DNA MODIFICATION

Also Published As

Publication number Publication date
WO2013068845A2 (en) 2013-05-16
EP2776560A2 (en) 2014-09-17
WO2013068845A3 (en) 2013-09-12
US20130210151A1 (en) 2013-08-15

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